CN104126613A - Method using ants to spread verticillium lecanii for control of tree trunk stomaphissinisalicis - Google Patents
Method using ants to spread verticillium lecanii for control of tree trunk stomaphissinisalicis Download PDFInfo
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- CN104126613A CN104126613A CN201410299908.7A CN201410299908A CN104126613A CN 104126613 A CN104126613 A CN 104126613A CN 201410299908 A CN201410299908 A CN 201410299908A CN 104126613 A CN104126613 A CN 104126613A
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- verticillium lecanii
- willow
- ant
- brown sugar
- long beak
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Abstract
The invention discloses a method using ants to spread verticillium lecanii for control of tree trunk stomaphissinisalicis; first, a lard and brown sugar mixture with the sugar content about 5-30% is prepared, after the lard and brown sugar mixture is condensed into a bulk, the lard and brown sugar mixture is cut into 0.5-2cm<3> small pieces, the small pieces are rolled in verticillium lecanii spore powder, so that the surface of the small pieces is fully covered with verticillium lecanii spore, and a verticillium lecanii spore release block is prepared; the verticillium lecanii spore release block is put in an inlet of an ant nest and an inlet of an ant path of a tree trunk for freely feeding to the ants, in the free feeding process, the verticillium lecanii spore may stick to epidermis, mouthparts and limbs, when the verticillium lecanii spore contacts stomaphissinisalicis hiding in the tree trunk, the verticillium lecanii spore may be transferred to the stomaphissinisalicis, and after the stomaphissinisalicis body surface is infected by the verticillium lecanii spore, the stomaphissinisalicis may die due to infection. The method has the advantages of good control effect, low cost and environmental protection.
Description
Technical field
The invention belongs to biological technical field, relate to one and utilize ant to propagate Verticillium lecanii
pandora neoaphidisthe large aphid of the control long beak of trunk willow
stomaphis sinisalicismethod.
Background technology
The large aphid of the long beak of willow is the multiple willows such as a kind of important Willow Pests, main harm white willow, almond willow, weeping willow, salix fragilis, Qinggang willow, smooth willow, korean willow.There is generation more than 10 in 1 year in this aphid, survives the winter in the bark gap of trunk bottom with adult.Start to top movable spring in next year, and 4-5 month amount reproduction is contained and sent out, and forms disaster.When a large amount of generation, the following drizzle of secreted honeydew, has a layer brown mucus on ground.Endanger more if aphid and adult are clustered on sprout branching place and spray, suck a liquid, secretion honeydew, often cause sooty mould occur, midsummer is less, form spring and autumn two occurance peaks.Part polypide can parasitize in the tree seam of willow, in scar place or root soil, be drawn onto the nutrition of the phloem absorption willow of trunk with lancet thorn, on trunk, generally have symbiosis ant to build Yi road with earth, at root, large aphid is directly hidden in soil and lives the long beak of willow, avoid natural enemy of aphid as the predation of the insects such as ladybug, Chrysopa, syrphus fly, more difficult infected insect pathogene simultaneously, is used agricultural chemicals to prevent and treat and is also difficult to prove effective, and difficulty of prevention and cure is very big.Meanwhile, the large aphid of the long beak of the willow of conceal type is its important channel of surviving the winter, and is also that the large aphid of the long beak of willow is difficult to one of major reason of preventing and treating.
Summary of the invention
The present invention is directed to above weak point, a kind of method of utilizing ant to propagate the large aphid of the Verticillium lecanii control long beak of trunk willow is provided, by Verticillium lecanii being propagated to the large aphid of the long beak of willow with the ant of the large aphid symbiosis of the long beak of willow, the large aphid body surface of the long beak of willow infects after Verticillium lecanii spore, susceptible death, reaches the control to the large aphid of the long beak of willow.
The technical solution adopted for the present invention to solve the technical problems is: a kind of method of utilizing ant to propagate the large aphid of the Verticillium lecanii control trunk concealment long beak of willow, includes following steps:
(1) prepare Verticillium lecanii conidial powder, can be by buying or solid fermentation cultivation acquisition;
(2) brown sugar lard piece first melts lard, slowly adds brown sugar to melt, and stirs evenly gently, makes brown sugar content account for 5-30%, cooling, make brown sugar lard piece after condensing, and brown sugar lard piece is cut into 0.5-2cm
3square fritter, in refrigerator, 4 DEG C of refrigerations are for subsequent use;
(3) the brown sugar lard piece being cut into small pieces is rolled in Verticillium lecanii conidial powder, make surface be stained with conidial powder, make Verticillium lecanii spores release piece;
(4) Verticillium lecanii spores release piece is placed on around the willow in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of the long beak of willow, ant takes food in the process of Verticillium lecanii spores release piece, Verticillium lecanii spore is sticked on body surface or mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of the long beak of willow contacts or takes food honeydew, Verticillium lecanii spore is passed to the large aphid of the long beak of willow, after the large aphid contact of the long beak of willow Verticillium lecanii spore, susceptible death.
Further, for the first time around the willow in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of the long beak of willow, placement Verticillium lecanii spores release piece is after 10 days, then places Verticillium lecanii spores release piece one time, control efficiency is better.
The present invention is mainly the symbiotic relation of having utilized between ant and the large aphid of the long beak of willow, utilize movable outside ant by the willow long beak large aphid of Verticillium lecanii spore dispersal to concealment in tree seam of causing a disease, only need to make Verticillium lecanii spores release piece and can realize the present invention, cost accounting is low, use Verticillium lecanii spore to cause that the large aphid of the long beak of willow falls ill and carry out the control of the large aphid of the long beak of willow, do not use other chemical agents, do not destroy ecotope, environmental protection;
Experimental results show that, around the willow in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of the long beak of willow, two minor ticks were placed Verticillium lecanii spores release piece after 20 days, the large aphid infection rate of the long beak of willow is 78%, and control efficiency is equally good with the effect of directly cutting tree road sprinkling Verticillium lecanii spore open.This method saves time, laborsaving, with low cost, and in advance without the Aphed population in investigation ant road, more without cutting ant road open, the large aphid of the long beak of willow is wherein killed, only need place Verticillium lecanii spores release piece at the ant road junction of the ant nest mouth of the large aphid symbiosis of the long beak of willow ant or trunk, just can reach the object of preventing and treating the large aphid of the long beak of willow, the method does not need the professional training both can complete operation, and can reach same control efficiency, there is very strong replicability.
Brief description of the drawings
Figure 1 shows that difference prevents and treats the control effect of method to the large aphid of the long beak of conceal type willow in trunk ant road;
Fig. 2 is depicted as the control effect of Imidacloprid to the large aphid of the long beak of conceal type willow in trunk ant road.
Embodiment
Embodiment 1
Utilize ant to propagate a method for the large aphid of the Verticillium lecanii control trunk concealment long beak of willow, include following steps:
(1) prepare Verticillium lecanii conidial powder, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 5%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 2cm
3square fritter, in refrigerator, 4 DEG C of refrigerations are for subsequent use;
(3) the brown sugar lard piece being cut into small pieces is rolled in Verticillium lecanii conidial powder, make surface be stained with conidial powder, make Verticillium lecanii spores release piece;
(4) Verticillium lecanii spores release piece is placed on around the willow in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of willow, for the first time around the willow in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of willow, place Verticillium lecanii spores release piece after 10 days, place again Verticillium lecanii spores release piece one time, ant takes food in the process of Verticillium lecanii spores release piece, Verticillium lecanii spore is sticked on body surface or mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of the long beak of willow contacts or takes food honeydew, Verticillium lecanii spore is passed to the large aphid of the long beak of willow, after the large aphid contact of the long beak of willow Verticillium lecanii spore, susceptible death.
Embodiment 2
Utilize ant to propagate a method for the large aphid of the Verticillium lecanii control trunk concealment long beak of willow, include following steps:
(1) prepare Verticillium lecanii conidial powder, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 30%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 0.5cm
3square fritter, in refrigerator, 4 DEG C of refrigerations are for subsequent use;
(3) the brown sugar lard piece being cut into small pieces is rolled in Verticillium lecanii conidial powder, make surface be stained with conidial powder, make Verticillium lecanii spores release piece;
(4) Verticillium lecanii spores release piece is placed on around the willow in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of willow, for the first time around the willow in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of willow, place Verticillium lecanii spores release piece after 10 days, place again Verticillium lecanii spores release piece one time, ant takes food in the process of Verticillium lecanii spores release piece, Verticillium lecanii spore is sticked on body surface or mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of the long beak of willow contacts or takes food honeydew, Verticillium lecanii spore is passed to the large aphid of the long beak of willow, after the large aphid contact of the long beak of willow Verticillium lecanii spore, susceptible death.
Embodiment 3
Utilize ant to propagate a method for the large aphid of the Verticillium lecanii control trunk concealment long beak of willow, include following steps:
(1) prepare Verticillium lecanii conidial powder, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 15%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 1cm
3square fritter, in refrigerator, 4 DEG C of refrigerations are for subsequent use;
(3) the brown sugar lard piece being cut into small pieces is rolled in Verticillium lecanii conidial powder, make surface be stained with conidial powder, make Verticillium lecanii spores release piece;
(4) Verticillium lecanii spores release piece is placed on around the willow in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of willow, for the first time around the willow in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of willow, place Verticillium lecanii spores release piece after 10 days, place again Verticillium lecanii spores release piece one time, ant takes food in the process of Verticillium lecanii spores release piece, Verticillium lecanii spore is sticked on body surface or mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of the long beak of willow contacts or takes food honeydew, Verticillium lecanii spore is passed to the large aphid of the long beak of willow, after the large aphid contact of the long beak of willow Verticillium lecanii spore, susceptible death.
Application example:
With method of the present invention, on white willow, apply, getting trunk has the willow in earth ant road, does respectively following processing: (1) utilizes the inventive method to carry the large aphid of the ball Verticillium lecanii spores release piece control long beak of willow by ant; (2) utilize method of the present invention, again place once placing for the first time 10 d after Verticillium lecanii spores release piece respectively; (3) concentration is 10
8the Verticillium lecanii spore of individual/ml; (4) cutting ant road spray concentration open is 10
8the Verticillium lecanii spore of individual/ml; (5) imidacloprid wettable powder of 1000 times 5% is diluted in the surface sprinkling of Yi road; (6) cut ant road open and spray the imidacloprid wettable powder of 1000 times 5% of dilution; (7) contrast, is left intact.Cut ant road open and investigate quantity or dead borer population and the healthy borer population of the infection Verticillium lecanii of the large aphid of the long beak of ant Dao Neiliu respectively at 10 d and 20d after processing, calculating infection rate (lethality).After the each processing of every processing, the time is chosen 3 willows and investigates.Cut ant road open and investigate infection number and the healthy borer population of the large aphid of the long beak of ant Dao Neiliu respectively at 10 d and 20d after processing, calculating infection rate.After the each processing of every processing, the time is chosen 3 willows and investigates.
Result of study is shown in Fig. 1 and Fig. 2.Result shows, 10d after processing, the effect of cutting ant road sprinkling Verticillium lecanii spore open is best, the susceptible of the large aphid of the long beak of willow reaches 66%, the control efficiency of secondly carrying Verticillium lecanii spore for ant ant, the infection rate of the large aphid of the long beak of willow can reach 40%, control group He Yi road surface sprinkling Verticillium lecanii spore group, and the large aphid of the long beak of willow is all without infecting.And place by secondary after the spores release piece 10d of Verticillium lecanii, the infection rate of the large aphid of the long beak of willow in concealment Yu Yi road can reach more than 70%, and secondary is placed rear 20 d, and the infection rate of the large aphid of the long beak of willow can reach 78%, control effect is remarkable, with cut open ant road spray Verticillium lecanii spore (
t=0.38,
p=0.72) or spray Imidacloprid (
t=0.07,
p=0.94) effect is without significant difference.
But place the Verticillium lecanii spores release piece of our invention by the ant road junction the large aphid symbiosis of the long beak of willow ant and ant nest mouth, than cut open ant road spray spore more save time, laborsaving, need not any professional knowledge get final product complete operation, be that a kind of large aphid of the long beak of willow that is easier to promotion and application prevents and treats method.
Claims (2)
1. utilize ant to propagate a method for the large aphid of the Verticillium lecanii control long beak of trunk willow, it is characterized in that, include following steps:
(1) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 5-30%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 0.5-2cm
3square fritter, in refrigerator, 4 DEG C of refrigerations are for subsequent use;
(2) the brown sugar lard piece being cut into small pieces is rolled in Verticillium lecanii conidial powder, make surface be stained with conidial powder, make Verticillium lecanii spores release piece;
(3) Verticillium lecanii spores release piece is placed on around the willow in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of the long beak of willow, ant takes food in the process of Verticillium lecanii spores release piece, Verticillium lecanii spore is sticked on body surface or mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of the long beak of willow contacts or takes food honeydew, Verticillium lecanii spore is passed to the large aphid of the long beak of willow, after the large aphid contact of the long beak of willow Verticillium lecanii spore, susceptible death.
2. a kind of method of utilizing ant to propagate the large aphid of the Verticillium lecanii control long beak of trunk willow according to claim 1, it is characterized in that, for the first time around the willow in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of the long beak of willow, place Verticillium lecanii spores release piece after 10 days, then place Verticillium lecanii spores release piece one time.
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| CN201410299908.7A CN104126613A (en) | 2014-06-30 | 2014-06-30 | Method using ants to spread verticillium lecanii for control of tree trunk stomaphissinisalicis |
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|---|---|---|---|
| CN201410299908.7A CN104126613A (en) | 2014-06-30 | 2014-06-30 | Method using ants to spread verticillium lecanii for control of tree trunk stomaphissinisalicis |
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| CN104126613A true CN104126613A (en) | 2014-11-05 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875512A (en) * | 2016-04-08 | 2016-08-24 | 泰山医学院 | Method for mixing nasonia vitripennis and verticillium lecanii to prevent flies |
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| CN101548669A (en) * | 2009-05-12 | 2009-10-07 | 上海市农业科学院 | Method for preventing and killing vegetable noctuid by cooperation of parasitic wasp and virus |
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2014
- 2014-06-30 CN CN201410299908.7A patent/CN104126613A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101548669A (en) * | 2009-05-12 | 2009-10-07 | 上海市农业科学院 | Method for preventing and killing vegetable noctuid by cooperation of parasitic wasp and virus |
| CN103783030A (en) * | 2013-12-26 | 2014-05-14 | 中国农业科学院植物保护研究所 | Method of controlling pests by combination of insect predator Carabussmaragdinus Fischer and Metarrhiziumanisopliae |
Non-Patent Citations (1)
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| 刘春来 等，: ""蜡蚧轮枝菌素对5种蚜虫毒杀作用的初步研究"", 《中国农学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875512A (en) * | 2016-04-08 | 2016-08-24 | 泰山医学院 | Method for mixing nasonia vitripennis and verticillium lecanii to prevent flies |
| CN105875512B (en) * | 2016-04-08 | 2018-10-12 | 泰山医学院 | A method of fusion Pupal parasite and Verticillium lecanii prevent flies |
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Application publication date: 20141105 |
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