CN104115709A - Method for utilizing ants to propagate verticillium lecanii so as to prevent and treat tree trunk cypress long-proboscis aphids - Google Patents
Method for utilizing ants to propagate verticillium lecanii so as to prevent and treat tree trunk cypress long-proboscis aphids Download PDFInfo
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- CN104115709A CN104115709A CN201410300242.2A CN201410300242A CN104115709A CN 104115709 A CN104115709 A CN 104115709A CN 201410300242 A CN201410300242 A CN 201410300242A CN 104115709 A CN104115709 A CN 104115709A
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Abstract
The invention discloses a method for utilizing ants to propagate verticillium lecanii so as to prevent and treat tree trunk cypress long-proboscis aphids. A lard oil and brown sugar mixture is firstly prepared, the brown sugar content accounts for about 5%-30%, the lard oil and brown sugar mixture is cut into 0.5-2cm<3> squares of small blocks after coagulating into blocks, and then the small blocks roll in verticillium lecanii spore powder so that the surfaces of the small blocks can be full of the verticillium lecanii spore powder, and spore release blocks are formed. The blocks are arranged at ant outlets and trunk ant way inlets of ant nests and used for the ants to freely take and eat, and the verticillium lecanii spore powder adheres to the skin, the mouthparts and limbs of the ants. When the verticillium lecanii spore powder is in contact with the cypress long-proboscis aphids hidden in ant ways, spores are propagated to the cypress long-proboscis aphids, the tree trunk cypress long-proboscis aphids get sick and die after the body surface of the cypress long-proboscis aphids are infected with the verticillium lecanii spores. The method for utilizing the ants to propagate the verticillium lecanii so as to prevent and treat the tree trunk cypress long-proboscis aphids is good in preventing and treating effect, low in cost and environment-friendly.
Description
Technical field
The invention belongs to biological technical field, relate to one and utilize ant to propagate Verticillium lecanii
verticillium lecaniithe large aphid of the control long beak of trunk cypress
cinara tuiafulinamethod.
Background technology
The large aphid of the long beak of cypress is a kind of important forestry pest, and main harm arbor-vitae, arbor-vitae, pencil cedar etc., assemble young stem surface with adult, nymph to suck, and often causes sooty mould, affects cypress growth.Part polypide can parasitize in the tree seam of cypress or scar place; be drawn onto the nutrition of the phloem absorption cypress of trunk with lancet thorn; produce close dew; ant sucks Mi Lu and the large aphid of the long beak of cypress forms symbiotic relation; ant is generally outside with earth build Yi road at the tree seam that has the large aphid parasitism of the long beak of cypress; protection aphid avoids predation or the pathogenic infection of the natural enemy insects such as ladybug, Chrysopa, syrphus fly, and therefore, the difficulty of prevention and cure of the large aphid of the long beak of cypress is very large.Meanwhile, the large aphid of the long beak of the cypress of conceal type is its important channel of surviving the winter, and can transfer in ant nest winter and survive the winter, and is the key factor of aphid breeding in the coming year, meanwhile, is also that the large aphid of the long beak of cypress is difficult to one of major reason of preventing and treating.
Summary of the invention
The present invention is directed to above weak point, a kind of method of utilizing ant to propagate the large aphid of the Verticillium lecanii control long beak of trunk cypress is provided, by Verticillium lecanii being propagated to the large aphid of the long beak of cypress with the ant of the large aphid symbiosis of the long beak of cypress, the large aphid body surface of the long beak of cypress is infected with after Verticillium lecanii spore, susceptible death, reaches the object that the large aphid of the long beak of cypress is carried out to biological control.
The technical solution adopted for the present invention to solve the technical problems is: a kind of method of utilizing ant to propagate the large aphid of the Verticillium lecanii control long beak of trunk cypress, includes following steps:
(1) prepare Verticillium lecanii conidial powder, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 5-30%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 0.5-2cm
3square fritter, in refrigerator, 4 DEG C of refrigerations are for subsequent use;
(3) the brown sugar lard piece being cut into small pieces is rolled in Verticillium lecanii conidial powder, make surface be stained with conidial powder, make Verticillium lecanii spores release piece;
(4) Verticillium lecanii spores release piece is placed on around the cypress in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of the long beak of cypress, ant can be attracted to take food by brown sugar, take food in process, Verticillium lecanii spore can stick on its body surface, mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of the long beak of cypress contacts or takes food honeydew, Verticillium lecanii spore is passed to the large aphid of the long beak of cypress, after the large aphid contact of the long beak of cypress Verticillium lecanii spore, susceptible death.
Further, for the first time around the cypress in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of the long beak of cypress, placement Verticillium lecanii spores release piece is after 10 days, then places Verticillium lecanii spores release piece one time, control efficiency is better.
The present invention is mainly the symbiotic relation of having utilized between ant and the large aphid of the long beak of cypress, utilize movable outside ant by the cypress long beak large aphid of Verticillium lecanii spore dispersal to concealment in tree seam of causing a disease, only need to make Verticillium lecanii spores release piece and can realize the present invention, cost accounting is cheap, uses Verticillium lecanii spore to cause the large aphid morbidity of the long beak of cypress, reaches the object that the large aphid of the long beak of cypress is carried out to biological control, do not use other chemical agents, do not destroy ecotope, environmental protection, and with low cost.Operator can operate without carrying out professional training, and the method has good repeatability and stability.
Experimental results show that, around the cypress in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of the long beak of cypress, two minor ticks were placed Verticillium lecanii spores release piece after 20 days, the large aphid infection rate of the long beak of cypress is 78%, and control efficiency is the same with the effect of directly cutting tree road sprinkling Verticillium lecanii spore open.This method saves time, laborsaving, with low cost, and in advance without the Aphed population in investigation ant road, more without cutting ant road open, the large aphid of the long beak of cypress is wherein killed, only need place at the ant road junction of the ant nest mouth of the large aphid symbiosis of the long beak of cypress ant or trunk Verticillium lecanii spores release piece, just can reach the object of the large aphid of the control long beak of cypress, the method does not need the professional training both can complete operation, and can reach same control efficiency, having very strong replicability, is a kind of large aphid biological control of the long beak of cypress new method that has promotion prospect.
Brief description of the drawings
Fig. 1 is depicted as difference and prevents and treats the control effect of method to the large aphid of the long beak of trunk ant Dao Neibai;
Figure 2 shows that the control effect of Imidacloprid to the large aphid of the long beak of conceal type cypress in trunk ant road.
Embodiment
embodiment 1
Utilize ant to propagate a method for the large aphid of the Verticillium lecanii control long beak of trunk cypress, include following steps:
(1) prepare Verticillium lecanii conidial powder, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 5%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 2cm
3square fritter, in refrigerator, 4 DEG C of refrigerations are for subsequent use;
(3) the brown sugar lard piece being cut into small pieces is rolled in Verticillium lecanii conidial powder, make surface be stained with conidial powder, make Verticillium lecanii spores release piece;
(4) Verticillium lecanii spores release piece is placed on around the cypress in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of cypress, for the first time around the cypress in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of cypress, place Verticillium lecanii spores release piece after 10 days, place again Verticillium lecanii spores release piece one time, ant can be attracted to take food by brown sugar, take food in process, Verticillium lecanii spore can stick in its body surface, on mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of the long beak of cypress contacts or takes food honeydew, Verticillium lecanii spore is passed to the large aphid of the long beak of cypress, after the large aphid contact of the long beak of cypress Verticillium lecanii spore, susceptible death.
Embodiment 2
Utilize ant to propagate a method for the large aphid of the Verticillium lecanii control long beak of trunk cypress, include following steps:
(1) prepare Verticillium lecanii conidial powder, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 30%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 0.5cm
3square fritter, in refrigerator, 4 DEG C of refrigerations are for subsequent use;
(3) the brown sugar lard piece being cut into small pieces is rolled in Verticillium lecanii conidial powder, make surface be stained with conidial powder, make Verticillium lecanii spores release piece;
(4) Verticillium lecanii spores release piece is placed on around the cypress in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of cypress, for the first time around the cypress in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of cypress, place Verticillium lecanii spores release piece after 10 days, place again Verticillium lecanii spores release piece one time, ant can be attracted to take food by brown sugar, take food in process, Verticillium lecanii spore can stick in its body surface, on mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of the long beak of cypress contacts or takes food honeydew, Verticillium lecanii spore is passed to the large aphid of the long beak of cypress, after the large aphid contact of the long beak of cypress Verticillium lecanii spore, susceptible death.
Embodiment 3
Utilize ant to propagate a method for the large aphid of the Verticillium lecanii control long beak of trunk cypress, include following steps:
(1) prepare Verticillium lecanii conidial powder, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 15%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 1cm
3square fritter, in refrigerator, 4 DEG C of refrigerations are for subsequent use;
(3) the brown sugar lard piece being cut into small pieces is rolled in Verticillium lecanii conidial powder, make surface be stained with conidial powder, make Verticillium lecanii spores release piece;
(4) Verticillium lecanii spores release piece is placed on around the cypress in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of cypress, for the first time around the cypress in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of the long beak of cypress, place Verticillium lecanii spores release piece after 10 days, place again Verticillium lecanii spores release piece one time, ant can be attracted to take food by brown sugar, take food in process, Verticillium lecanii spore can stick in its body surface, on mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of the long beak of cypress contacts or takes food honeydew, Verticillium lecanii spore is passed to the large aphid of the long beak of cypress, after the large aphid contact of the long beak of cypress Verticillium lecanii spore, susceptible death.
Application example:
With method of the present invention, on the cypress of Mount Taishan, apply, getting trunk has the cypress in earth ant road, does respectively following processing: (1) utilizes the inventive method to carry the large aphid of the Verticillium lecanii spores release piece control long beak of cypress by ant; (2) utilize method of the present invention, again place once placing for the first time 10 d after Verticillium lecanii spores release piece respectively; (3) concentration is 10
8the Verticillium lecanii spore of individual/ml; (4) cutting ant road spray concentration open is 10
8the Verticillium lecanii spore of individual/ml; (5) imidacloprid wettable powder of 1000 times 5% is diluted in the surface sprinkling of Yi road; (6) cut ant road open and spray the imidacloprid wettable powder of 1000 times 5% of dilution; (7) contrast, is left intact.Cut ant road open and investigate quantity or dead borer population and the healthy borer population of the infection Verticillium lecanii of the large aphid of the long beak of ant Dao Neibai respectively at 10 d and 20d after processing, calculating infection rate (lethality).After the each processing of every processing, the time is chosen 3 cypresses and investigates.
Result of study is shown in Fig. 1.Result shows, 10d after processing, the effect of cutting ant road sprinkling Verticillium lecanii spore open is best, the susceptible of the large aphid of the long beak of cypress reaches 66%, the control efficiency of secondly carrying Verticillium lecanii spore for ant ant, the infection rate of the large aphid of the long beak of cypress can reach 40%, control group He Yi road surface sprinkling Verticillium lecanii spore group, and the large aphid of the long beak of cypress is all without infecting.And place by secondary after the spores release piece 10d of Verticillium lecanii, the infection rate of the large aphid of the long beak of cypress in concealment Yu Yi road can reach more than 70%, and secondary is placed rear 20 d, and the infection rate of the large aphid of the long beak of cypress can reach 78%, control effect is remarkable, with cut open ant road spray Verticillium lecanii spore (
t=1.88,
p=0.13) (infection rate of the large aphid of the long beak of cypress is 75%) or Imidacloprid (
t=0.63,
p=0.55) effect of (lethality is 76.6%) is without significant difference, and control efficiency is sprayed the control efficiency (lethality is 4%) of Imidacloprid far above surface, Yi road or root simultaneously.
Cutting ant road open, to spray Imidacloprid workload large, and environment is had a certain impact, time-consuming, effort, and cost is higher, and for having inconvenient traffic or the area of water scarcity, the operability of spraying Imidacloprid is not strong.Comparatively speaking, place the spores release piece of Verticillium lecanii and be the more method of economy, environmental protection, and control efficiency is pretty good, need not any professional knowledge get final product complete operation, is that a kind of large aphid of the long beak of cypress that is easier to promotion and application prevents and treats method.
Claims (2)
1. utilize ant to propagate a method for the large aphid of the Verticillium lecanii control long beak of trunk cypress, it is characterized in that, include following steps:
(1) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 5%-30%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 0.5-2cm
3square fritter, in refrigerator, 4 DEG C of refrigerations are for subsequent use;
(2) the brown sugar lard piece being cut into small pieces is rolled in Verticillium lecanii conidial powder, make surface be stained with conidial powder, make Verticillium lecanii spores release piece;
(3) Verticillium lecanii spores release piece is placed on around the cypress in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of the long beak of cypress, ant can be attracted by brown sugar, taking food in the process of Verticillium lecanii spores release piece, Verticillium lecanii spore is sticked on body surface, mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of the long beak of cypress contacts or takes food honeydew, Verticillium lecanii spore is passed to the large aphid of the long beak of cypress, after the large aphid contact of the long beak of cypress Verticillium lecanii spore, susceptible death.
2. a kind of method of utilizing ant to propagate the large aphid of the Verticillium lecanii control long beak of trunk cypress according to claim 1, it is characterized in that, for the first time around the cypress in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of the long beak of cypress, place Verticillium lecanii spores release piece after 10 days, then place Verticillium lecanii spores release piece one time.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104838892A (en) * | 2015-06-02 | 2015-08-19 | 云南农业大学 | Method for reproducing verticillum lecanii and recovering vigor of verticillum lecanii by using larvas of argyrogramma agnata |
| CN113068548A (en) * | 2021-03-30 | 2021-07-06 | 重庆市农业科学院 | Method for preventing and controlling sucking pests by using ants carrying beauveria bassiana |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1216219A (en) * | 1997-11-05 | 1999-05-12 | 中国科学院武汉病毒研究所 | Pest-insect preventing and controlling method utilizing parasitic wasp to transfer virus |
| CN1432276A (en) * | 2002-01-10 | 2003-07-30 | 武增强 | Adhesive lure for killing roach and ant |
| CN1587376A (en) * | 2004-08-06 | 2005-03-02 | 中国农业大学 | Lecanium verticillin and cultivating method and use |
| CN1631091A (en) * | 2005-01-26 | 2005-06-29 | 北京市农林科学院 | Biological control method for orchard ecology |
| CN101444226A (en) * | 2008-12-26 | 2009-06-03 | 沈阳农业大学 | Recipe of Verticillium lecanii(Zimmerman)Viegas water dispersible granules used for preventing and controlling aphids and greenhouse whiteflies and preparation method |
| CN103271022A (en) * | 2013-06-05 | 2013-09-04 | 新疆林科院森林生态研究所 | Method for controlling melanophila picta pallas larvae through fusarium proliferatum carried on sclerodermus pupariae |
| CN103548798A (en) * | 2013-10-29 | 2014-02-05 | 武汉大学 | Method and device for microbial pest control based on light luring |
-
2014
- 2014-06-30 CN CN201410300242.2A patent/CN104115709A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1216219A (en) * | 1997-11-05 | 1999-05-12 | 中国科学院武汉病毒研究所 | Pest-insect preventing and controlling method utilizing parasitic wasp to transfer virus |
| CN1432276A (en) * | 2002-01-10 | 2003-07-30 | 武增强 | Adhesive lure for killing roach and ant |
| CN1587376A (en) * | 2004-08-06 | 2005-03-02 | 中国农业大学 | Lecanium verticillin and cultivating method and use |
| CN1631091A (en) * | 2005-01-26 | 2005-06-29 | 北京市农林科学院 | Biological control method for orchard ecology |
| CN101444226A (en) * | 2008-12-26 | 2009-06-03 | 沈阳农业大学 | Recipe of Verticillium lecanii(Zimmerman)Viegas water dispersible granules used for preventing and controlling aphids and greenhouse whiteflies and preparation method |
| CN103271022A (en) * | 2013-06-05 | 2013-09-04 | 新疆林科院森林生态研究所 | Method for controlling melanophila picta pallas larvae through fusarium proliferatum carried on sclerodermus pupariae |
| CN103548798A (en) * | 2013-10-29 | 2014-02-05 | 武汉大学 | Method and device for microbial pest control based on light luring |
Non-Patent Citations (1)
| Title |
|---|
| 陈晓明: "利用隐翅虫携带病原菌防治松墨天牛技术研究", 《中国优秀硕士学位论文全文数据库_农业科技辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104838892A (en) * | 2015-06-02 | 2015-08-19 | 云南农业大学 | Method for reproducing verticillum lecanii and recovering vigor of verticillum lecanii by using larvas of argyrogramma agnata |
| CN104838892B (en) * | 2015-06-02 | 2017-04-12 | 云南农业大学 | Method for reproducing verticillum lecanii and recovering vigor of verticillum lecanii by using larvas of argyrogramma agnata |
| CN113068548A (en) * | 2021-03-30 | 2021-07-06 | 重庆市农业科学院 | Method for preventing and controlling sucking pests by using ants carrying beauveria bassiana |
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