CN104145941A - Method for preventing and controlling hidden type trunk Cinara tujafilina by using ants to spread Pandora neoaphidis - Google Patents
Method for preventing and controlling hidden type trunk Cinara tujafilina by using ants to spread Pandora neoaphidis Download PDFInfo
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- CN104145941A CN104145941A CN201410300109.7A CN201410300109A CN104145941A CN 104145941 A CN104145941 A CN 104145941A CN 201410300109 A CN201410300109 A CN 201410300109A CN 104145941 A CN104145941 A CN 104145941A
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- aphid
- pestilence
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Abstract
The invention discloses a method for preventing and controlling hidden type trunk Cinara tujafilina by using ants to spread Pandora neoaphidis. The method comprises the steps that a brown sugar and lard oil block is prepared, wherein brown sugar accounts for 5%-20% of the block; after condensation blocking, the block is cut into small pieces which are 0.5-2 cm<3> in size, and the small pieces are made to roll in Pandora neoaphidis spore powder to become spore release blocks; the spore release blocks are placed on an outlet of an ant nest and an inlet of an ant path on a trunk to be eaten freely by ants, Pandora neoaphidis spores can adhere to the cuticula, mouthparts and limbs of the ants during eating and can be transferred to Cinara tujafilina when in contact with Cinara tujafilina hidden in the ant path, and Cinara tujafilina can be infected with a disease and die after the body surface of Cinara tujafilina is infected with the Pandora neoaphidis spores. The method is good in prevention and control effect, low in cost and environmentally friendly.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of ant propagation aphid pestilence of utilizing mould
pandora neoaphidisthe large aphid of control trunk conceal type cypress
cinara tuiafulinamethod.
Background technology
The large aphid of cypress is a kind of important forestry pest, and main harm arbor-vitae, arbor-vitae, pencil cedar etc., assemble young stem surface with adult, nymph to suck, and often causes sooty mould, affects cypress growth.Part polypide can parasitize in the tree seam of cypress or scar place; with lancet thorn, be drawn onto the nutrition of the phloem absorption cypress of trunk; produce close dew; ant sucks Mi Lu and the large aphid of cypress forms symbiotic relation; ant is generally built Yi road in the outside of tree seam with earth; protection aphid avoids predation or the pathogenic infection of the natural enemy insects such as ladybug, Chrysopa, syrphus fly, so difficulty of prevention and cure is very large.Meanwhile, the large aphid of the cypress of conceal type is its important channel of surviving the winter, and can transfer in ant nest winter and survive the winter, and is the key factor of aphid breeding in the coming year, meanwhile, is also that the large aphid of cypress is difficult to one of major reason of preventing and treating.
Summary of the invention
The present invention is directed to above weak point, a kind of method of utilizing ant to propagate the large aphid of aphid pestilence mould control trunk conceal type cypress is provided, by the ant with the large aphid symbiosis of cypress by the mould propagation of aphid pestilence to the large aphid of cypress, the large aphid body surface of cypress infects after the mould spore of aphid pestilence, susceptible death, reaches the control to the large aphid of cypress.
The technical solution adopted for the present invention to solve the technical problems is: a kind of method of utilizing ant to propagate the large aphid of aphid pestilence mould control trunk conceal type cypress, includes following steps:
(1) prepare the mould conidial powder of aphid pestilence, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 5-20%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 0.5-2cm
3square fritter, in refrigerator, 4 ℃ of refrigerations are standby;
(3) the brown sugar lard piece being cut into small pieces is rolled in the mould conidial powder of aphid pestilence, make surface be stained with conidial powder, make the mould spores release piece of aphid pestilence;
(4) the mould spores release piece of aphid pestilence is placed on around the cypress in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of cypress, ant takes food in the process of the mould spores release piece of aphid pestilence, the mould spore of aphid pestilence is sticked on body surface or mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of cypress contacts or takes food honeydew, the mould spore of aphid pestilence is passed to the large aphid of cypress, after the mould spore of the large aphid contact aphid pestilence of cypress, susceptible death.
Further, for the first time around the cypress in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of cypress, place the mould spores release piece of aphid pestilence after 10 days, then place the mould spores release piece of aphid pestilence one time, control efficiency is better.
The present invention is mainly the symbiotic relation of having utilized between ant and the large aphid of cypress, utilize movable outside ant by the cypress large aphid of the mould spore dispersal of aphid pestilence to concealment in tree seam of causing a disease, only need to make the mould spores release piece of aphid pestilence and can realize the present invention, cost accounting is low, use the mould spore of aphid pestilence to cause that the large aphid morbidity of cypress carries out the control of the large aphid of cypress, do not use other chemical agents, do not destroy ecotope, environmental protection;
Experimental results show that, around the cypress in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of cypress, two minor ticks were placed the mould spores release piece of aphid pestilence after 20 days, and the large aphid infection rate of cypress is 78%, and control efficiency is equally good with the effect of directly cutting the mould spore of tree road sprinkling aphid pestilence open.This method saves time, laborsaving, with low cost, and in advance without the Aphed population in investigation ant road, more without cutting ant road open, the large aphid of cypress is wherein killed, only need be placed the mould spores release piece of aphid pestilence at the ant nest mouth of the large aphid symbiosis of cypress ant or the ant road junction of trunk, just can reach the object of the large aphid of control cypress, the method does not need the professional training both can complete operation, and can reach same control efficiency, and there is very strong replicability, be a kind of large aphid biological control of cypress new method that has promotion prospect.
Accompanying drawing explanation
Figure 1 shows that the mould difference of aphid pestilence prevents and treats the control effect of method to the large aphid of conceal type cypress in trunk ant road;
Fig. 2 is depicted as the control effect of Imidacloprid to the large aphid of conceal type cypress in trunk ant road.
Embodiment
Embodiment 1
Utilize ant to propagate a method for the large aphid of aphid pestilence mould control trunk conceal type cypress, include following steps:
(1) prepare the mould conidial powder of aphid pestilence, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 5%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 2cm
3square fritter, in refrigerator, 4 ℃ of refrigerations are standby;
(3) the brown sugar lard piece being cut into small pieces is rolled in the mould conidial powder of aphid pestilence, make surface be stained with conidial powder, make the mould spores release piece of aphid pestilence;
(4) the mould spores release piece of aphid pestilence is placed on around the cypress in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of cypress, placing for the first time the mould spores release piece of aphid pestilence after 10 days, place again the mould spores release piece of aphid pestilence one time, ant can be attracted to take food by brown sugar, ant takes food in the process of the mould spores release piece of aphid pestilence, the mould spore of aphid pestilence is sticked on body surface or mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of cypress contacts or takes food honeydew, the mould spore of aphid pestilence is passed to the large aphid of cypress, after the mould spore of the large aphid contact aphid pestilence of cypress, susceptible death.
Embodiment 2
Utilize ant to propagate a method for the large aphid of aphid pestilence mould control trunk conceal type cypress, include following steps:
(1) prepare the mould conidial powder of aphid pestilence, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 20%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 0.5cm
3square fritter, in refrigerator, 4 ℃ of refrigerations are standby;
(3) the brown sugar lard piece being cut into small pieces is rolled in the mould conidial powder of aphid pestilence, make surface be stained with conidial powder, make the mould spores release piece of aphid pestilence;
(4) the mould spores release piece of aphid pestilence is placed on around the cypress in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of cypress, placing for the first time the mould spores release piece of aphid pestilence after 10 days, place again the mould spores release piece of aphid pestilence one time, ant can be attracted to take food by brown sugar, ant takes food in the process of the mould spores release piece of aphid pestilence, the mould spore of aphid pestilence is sticked on body surface or mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of cypress contacts or takes food honeydew, the mould spore of aphid pestilence is passed to the large aphid of cypress, after the mould spore of the large aphid contact aphid pestilence of cypress, susceptible death.
Embodiment 3
Utilize ant to propagate a method for the large aphid of aphid pestilence mould control trunk conceal type cypress, include following steps:
(1) prepare the mould conidial powder of aphid pestilence, can be by buying or solid fermentation cultivation acquisition;
(2) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 12%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 1cm
3square fritter, in refrigerator, 4 ℃ of refrigerations are standby;
(3) the brown sugar lard piece being cut into small pieces is rolled in the mould conidial powder of aphid pestilence, make surface be stained with conidial powder, make the mould spores release piece of aphid pestilence;
(4) the mould spores release piece of aphid pestilence is placed on around the cypress in trunk You Yi road, ant road junction and formicary mouth with the ant of the large aphid symbiosis of cypress, placing for the first time the mould spores release piece of aphid pestilence after 10 days, place again the mould spores release piece of aphid pestilence one time, ant can be attracted to take food by brown sugar, ant takes food in the process of the mould spores release piece of aphid pestilence, the mould spore of aphid pestilence is sticked on body surface or mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of cypress contacts or takes food honeydew, the mould spore of aphid pestilence is passed to the large aphid of cypress, after the mould spore of the large aphid contact aphid pestilence of cypress, susceptible death.
Application example:
With method of the present invention, on the cypress of Mount Taishan, apply, get the cypress that trunk has earth ant road, do respectively following processing: (1) utilizes the inventive method to carry the large aphid of the mould spores release piece control cypress of aphid pestilence by ant; (2) utilize method of the present invention, after placing for the first time the mould spores release piece of aphid pestilence, 10 d place once again respectively; (3) surface sprinkling concentration in Yi road is 10
8the mould spore of aphid pestilence of individual/ml; (4) cutting ant road spray concentration open is 10
8the mould spore of aphid pestilence of individual/ml; (5) imidacloprid wettable powder of 1000 times 5% is diluted in the surface sprinkling of Yi road; (6) cut the imidacloprid wettable powder that ant road sprays 1000 times 5% of dilution open; (7) contrast, is left intact.Respectively at 10 d after processing and 20d, cut infection number and the healthy borer population that the large aphid of ant Dao Neibai is investigated in ant road open, calculate infection rate.Every processing, rear time of each processing are chosen 3 cypresses and investigate.
Result of study is shown in Fig. 1 and Fig. 2.Result shows, 10d after processing, the effect of cutting the mould spore of ant road sprinkling aphid pestilence open is best, the susceptible of the large aphid of cypress reaches 66%, the control efficiency of secondly carrying the mould spore of aphid pestilence for ant ant, the infection rate of the large aphid of cypress can reach 40%, the mould spore group of control group He Yi road surface sprinkling aphid pestilence, and the large aphid of cypress is all without infecting.And place after the mould spores release piece 10d of aphid pestilence by secondary, the infection rate of the large aphid of cypress in concealment Yu Yi road can reach more than 70%, and secondary is placed rear 20 d, and the infection rate of the large aphid of cypress can reach 78%, control effect is remarkable, with cut open ant road spray the mould spore of aphid pestilence (infection rate is 75%) (
t=0.63,
p=0.55) or Imidacloprid (lethality is 76%) (
t=0.11,
p=0.90) effect is without significant difference.
But by the ant road junction the large aphid symbiosis of cypress ant and ant nest mouth, place the mould spores release piece of aphid pestilence of our invention, than cut open ant road spray spore more save time, laborsaving, need not any professional knowledge get final product complete operation, be that a kind of large aphid of cypress that is easier to promotion and application prevents and treats method.
Claims (2)
1. utilize ant to propagate a method for the large aphid of aphid pestilence mould control trunk conceal type cypress, it is characterized in that, include following steps:
(1) prepare brown sugar lard piece and first lard is melted, slowly add brown sugar to melt, stir evenly gently, make brown sugar content account for 5-20%, cooling, make brown sugar lard piece after condensing, brown sugar lard piece is cut into 0.5-2cm
3square fritter, in refrigerator, 4 ℃ of refrigerations are standby;
(2) the brown sugar lard piece being cut into small pieces is rolled in the mould conidial powder of aphid pestilence, make surface be stained with conidial powder, make the mould spores release piece of aphid pestilence;
(3) the mould spores release piece of aphid pestilence is placed on around the cypress in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of cypress, ant takes food in the process of the mould spores release piece of aphid pestilence, the mould spore of aphid pestilence is sticked on body surface or mouthpart or four limbs, when in ant Yu Yi road, the hiding large aphid of cypress contacts or takes food honeydew, the mould spore of aphid pestilence is passed to the large aphid of cypress, after the mould spore of the large aphid contact aphid pestilence of cypress, susceptible death.
2. a kind of method of utilizing ant to propagate the large aphid of aphid pestilence mould control trunk conceal type cypress according to claim 1, it is characterized in that, for the first time around the cypress in trunk You Yi road, with ant road junction and the formicary mouth of the ant of the large aphid symbiosis of cypress, place the mould spores release piece of aphid pestilence after 10 days, then place the mould spores release piece of aphid pestilence one time.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410300109.7A CN104145941A (en) | 2014-06-30 | 2014-06-30 | Method for preventing and controlling hidden type trunk Cinara tujafilina by using ants to spread Pandora neoaphidis |
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|---|---|---|---|
| CN201410300109.7A CN104145941A (en) | 2014-06-30 | 2014-06-30 | Method for preventing and controlling hidden type trunk Cinara tujafilina by using ants to spread Pandora neoaphidis |
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Application publication date: 20141119 |
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