CN104096154A - Traditional Chinese medicine composition for treating oral ulcer - Google Patents
Traditional Chinese medicine composition for treating oral ulcer Download PDFInfo
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- CN104096154A CN104096154A CN201410394180.6A CN201410394180A CN104096154A CN 104096154 A CN104096154 A CN 104096154A CN 201410394180 A CN201410394180 A CN 201410394180A CN 104096154 A CN104096154 A CN 104096154A
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Abstract
The invention discloses a traditional Chinese medicine composition for treating oral ulcer, which belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition for treating oral ulcer is prepared from the following raw materials in parts by weight: 10-12 parts of dandelion, 8-10 parts of alpinia katsumadai, 6-9 parts of radix paeoniae alba, 6-8 parts of poria cocos, 5-7 parts of mint, 5-9 parts of andrographis paniculata, 10-12 parts of fructus aurantii, 10-15 parts of hawthorn, 8-10 parts of radix bupleuri, 10-15 parts of honeysuckle, 7-9 parts of scutellaria baicalensis, 3-6 parts of salvia miltiorrhiza, 5-7 parts of lotus leaves, 5-9 parts of acanthopanax, 3-5 parts of bunge cherry seed and 10-12 parts of liquorice. The traditional Chinese medicine composition is great in treatment effect in the aspect of treating and preventing oral ulcer, and remarkable in clinical promotion value.
Description
Technical field
The invention belongs to medical technical field, be specifically related to the application of a kind of Chinese medicine composition and conduct preparation treatment or preventing canker sore medicine.
Background technology
Oral ulcer, is called again " aphtha ", is a kind of oral mucosa limitation ulcerative lesions that outbreak is feature periodically repeatedly of take, can spontaneous recovery, can occur in any position of oral mucosa., there is single or a plurality of circular or oval ulcer differing in size in the mucosa of locating with lip, cheek, soft palate or the gums etc. in oral cavity, the greyish white or blue pseudomembrane ulcer of surface coverage, and clear border, around mucosa is red and micro-swollen, and local causalgia is principal character.It easily recurs, the cycle is short, and very easily infects, as treatment not in time, and Relapse rate outbreak, it is lasting to delay, and also can transfer " behcet's syndrome " to, even involves internal organs, has caused great misery to patient's work and life.
Oral ulcer belongs to the traditional Chinese medical science " aphtha ", " erosion of the oral mucosa " category.Though aphtha is born in mouth, there is substantial connection with internal organs.The traditional Chinese medical science thinks, The mouth is the body opening or window of the spleen, the tongue is the orifice to the heart, the kidney pulse connect pharynx be tongue this, two cheeks and gums belong to stomach and large intestine, conception vessel, governor vessel are all gone up network oral cavity words, show that the generation of aphtha and the five internal organs are in close relations.Li Dongyuan is said in < < Treatise on the spleen and stomach > >: " YIN-fire also; arise from the part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels; it lies in the heart for both taste weakness of QI; insufficiency of primordial QI; and heart-fire is solely contained, heart-fire person, the not main order of the heart, fire is for it mutually ", " gastropathy is breathed hard; spirit less and raw large heat, sometimes up its face that solely burns of stomach-fire " treatment can select treatment by Chinese herbs method.
The recurrent oral ulceration cause of disease is more complicated, and clinical treatment will be according to different inducements, treatment pointedly, the curative effect that could obtain.As: remove psychentonia pressure, calmness, sleep peacefully, smoking cessation, wine etc.; Local for ulcer, can adopt part to embrocate pain relieving, antiinflammatory, promotes the medicine healing.As: cod-liver oil paste, prednisone paste, nourishing yin shengjisan, ulcer ointment etc., or local anaesthetics is embrocated in front part on the feed, to ease the pain; Or adopt hormone and antibiotic therapy with respite patient's misery, but the infringement that the toxic and side effects such as dysbacteriosis that easily form cause health.In dental care medicine on market, majority is that Chinese medicine and western medicine is in conjunction with preventing and treat oral disease now, and take Western medicine as main composition, though there is good bacteriostasis and obtained good clinical short-term curative effects, but Western medicine side effect greatly, easily produces drug resistance, there is the high and adverse effect of relapse rate, severe patient even may cause that dysbacteriosis, oral mucosa strip off and superposition sense, and action target spot is single, and long-term taking can affect patient's health status.Therefore researching and developing the effective medicine of a kind of oral ulcer is still of great practical significance.
CN101897726A discloses a kind of Chinese medicine powder for the treatment of oral ulcer, consists of: Vermilion 80-100 divides, Endoconcha Sepiae 100-200 part the component of following weight portion.This medicine cures mainly: oral ulcer, accumulation of heat in the heart and spleen type oral mucosa disease.
CN103127410 discloses a kind of Chinese medicine composition for the treatment of oral ulcer, by following raw materials according Chinese medicine composition, is formed: Radix Rehmanniae 15g, wheat 15g, Fructus Forsythiae 10g, Fructus Gardeniae 9g, Rhizoma Dioscoreae 30g, Flos Lonicerae 15g, Radix Paeoniae Alba 15g, Rhizoma Coptidis 10g, Cortex Moutan 15g, Folium Bambusae 10g, Radix Bupleuri 10g, Radix Scutellariae 12g, Cortex Phellodendri 15g, Rhizoma Belamcandae 6g, Herba Violae 10g, Herba Andrographis 9g, Radix Sophorae Tonkinensis 12g.During treatment, get above-mentioned Chinese medicine, add 1500ml water, slow fire boiling 30 minutes, filtering residue is made decoction, every day potion, seven days courses for the treatment of.
CN102430053A discloses a kind of Chinese medicine for the treatment of oral ulcer, the component of the material medicine of following weight proportion, consists of: Radix Rehmanniae 15-20g, Radix Scrophulariae 15-20g, Radix Ophiopogonis 15-20g, Natrii Sulfas 6-10g, Radix Et Rhizoma Rhei 6-10g, Radix Codonopsis 15-20g, Rhizoma Atractylodis Macrocephalae 10-15g, Rhizoma Cimicifugae 10-12g, Rhizoma Coptidis 6-10g, Cortex Moutan 15-20g, Radix Angelicae Sinensis 10-15g, Gypsum Fibrosum 20-30g, Pseudobulbus Bletillae (Rhizoma Bletillae) 15-20g.
Summary of the invention
The undesirable present situation of therapeutic effect for current oral ulcer clinical treatment medicine, first object of the present invention is to provide a kind of Chinese medicine composition for the treatment of oral ulcer, and this Chinese medicine composition has good therapeutic effect aspect treatment acute oral ulcer, chronic stomatocace and anaphylaxis oral ulcer.Chinese medicine composition of the present invention, is made by the raw material of following weight portion: Herba Taraxaci 10-12 part, Semen Alpiniae Katsumadai 8-10 part, Radix Paeoniae Alba 6-9 part, Poria 6-8 part, Herba Menthae 5-7 part, Herba Andrographis 5-9 part, Fructus Aurantii 10-12 part, Fructus Crataegi 10-15 part, Radix Bupleuri 8-10 part, Flos Lonicerae 10-15 part, Radix Scutellariae 7-9 part, Radix Salviae Miltiorrhizae 3-6 part, Folium Nelumbinis 5-7 part, Radix Et Caulis Acanthopanacis Senticosi 5-9 part, Semen Pruni 3-5 part, Radix Glycyrrhizae 10-12 part.
Dandelion Compositae herbaceos perennial.In Herba Taraxaci plant, contain the multiple health-nutrition compositions such as taraxol, taraxacin, choline, organic acid, inulin, have the effects such as diuresis, cathartic, jaundice eliminating subcutaneous ulcer, function of gallbladder promoting.According to < < Compendium of Materia Medica > >, record, the flat sweet and slightly bitter taste of its property, has heat-clearing and toxic substances removing, dispersing swelling and dissipating binds and galgctogogue action, very effective to treatment mastitis.No matter decoct juice oral, or smash to mud external application, effect all had.In addition, Herba Taraxaci also has the effects such as diuresis, cathartic, jaundice eliminating subcutaneous ulcer, function of gallbladder promoting, is widely used in clinical.
Semen Alpiniae Katsumadai this product is the dry near mature seed of zingiberaceous plant Semen Alpiniae Katsumadai Alpinia katsumadai Hayata.Summer, Qiu Erji gather, and shine to ninety percent dry, or water slightly scald, and shine to half-dried, remove peel, take out seed group, dry.Nature and flavor are pungent, temperature.Return spleen, stomach warp.The circulation of qi promoting of energy dampness, warming middle-JIAO to arrest vomiting.For internal retention of cold wetness, distension and fullness in the abdomen cold type of pain, heating installation vomiting, anorexia.
The Radix Paeoniae Alba enters liver, spleen channel, has nourishing blood to suppress the hyperactive liver, slow middle pain relieving, and yin fluid astringing is received the effect of antiperspirant.Cure mainly the breast abdomen pain over the hypochondriac region, dysentery stomachache, spontaneous sweating, fever due to yin deficiency, menoxenia, metrorrhagia, is with inferior disease.Herba Menthae is one of Chinese conventional Chinese medicine.It is pungent cool property sweating antipyretic medicine, controls influenza, headache, conjunctival congestion, fever of the body, throat, the dental bed disease such as swell and ache.External can training the mind dysmenorrhoea, skin pruritus, erythra and eczema etc.The Herba Menthae of light friend Greenhouse Fruit Picking is again the fresh vegetable on dining table in the Spring Festival.Clearly good to eat.Usually with Herba Menthae for tea, improving eyesight clears away heart-fire.Modern medicine proof Herba Menthae has very strong antiviral and antibacterial activity, and has certain antiallergic activity.
Poria is the dry sclerotia of On Polyporaceae Poria Poria cocos (Schw.) Wolf.More than 7～JIUYUE, excavate, after digging out, remove silt, bank up after " diaphoresis ", spread out and dry in the air to dry tack free, then " diaphoresis ", after extremely now wrinkle, the large portion of internal moisture scatter and disappear for several times repeatedly, dry in the shade, be called " Poria "; Or fresh Poria is pressed to different parts cutting, and dry in the shade, be called " Poria piece " and " Poria sheet ".Nature and flavor are sweet, light, flat.GUIXIN, lung, spleen, kidney channel.Function promoting diuresis to eliminate damp pathogen, spleen invigorating, mind calming.For edema oliguria, phlegm retention vertigo and palpitation, insufficiency of the spleen lack of appetite, have loose bowels in loose stool, irritability, palpitation with fear insomnia.
Fructus Aurantii is the dry immature fruit of rutaceae Citrus aurantium Linn. Citrus aurantium L. and variety thereof.Gather when peel is still green July, from middle part crosscut, is two halves, dries or cold drying.Nature and flavor are bitter, pungent, sour, are slightly cold.Return spleen, stomach warp.Energy regulating the flow of QI to ease the stomach, the stagnant relieving distension of row.For the breast side of body stagnation of QI, distension pain, accumulation of food in the stomach and intes tine due to indigestion, stops in phlegm retention, visceroptosis.
Flos Lonicerae is Caprifoliaceae woodbine Radix Ophiopogonis and congener dry flower or the first flower of opening of band.Flos Lonicerae is described as the good medicine of heat-clearing and toxic substances removing from ancient times.The sweet cold of its property fragrance, clearing away heat with drugs sweet in flavor and cold in nature and not injuring one's stomach, fragrance thoroughly reaches and can be eliminating evil.Flos Lonicerae can dispelling wind-heat, kind removing summer-heat blood poison also, and for various febrile diseases, as fever of the body, dermexanthesis, send out the cards such as speckle, pyretic toxicity carbuncle sore, laryngopharynx swelling and pain, all effect is remarkable.Modern medicine shows, Flos Lonicerae is containing cyclohexanhexanol, flavonoid, inositol, Saponin and tannin etc., there is broad-spectrum antibacterial action, the various pathogens such as staphylococcus aureus, dysentery bacterium are all had to stronger inhibitory action, the multiple pathogenic microorganisms such as leptospira, influenza virus and pathogenic moulds are also had to inhibitory action.In addition, Flos Lonicerae also has obvious antiinflammatory and refrigeration function, and Flos Lonicerae water and steeping in wine liquid have obvious lethal effect to tentative tumor cell.
Radix Scutellariae another name Radix Scutellariae, tsuchikane tea root, be labiate, with root, is used as medicine.Have heat clearing and damp drying, removing heat from blood is antiabortive, detoxicating functions.Cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle.Rhizoma Atractylodis have drying damp and strengthening spleen, expelling wind and cold, and the effect of improving eyesight, for dampness taste, distension and fullness in the abdomen, cold-damp leucorrhea, damp-warm diseases and damp invasion of lower energizer, foot swelling and pain in knee, flaccidity are soft unable.Control dampness taste, and see the thick disease such as turbid of distension and fullness in the abdomen, inappetence, fatigue and weakness, white and greasy fur, the compatibilities such as normal and Cortex Magnoliae Officinalis, Pericarpium Citri Reticulatae are applied.
Folium Nelumbinis is the leaf of nymphaeaceae plant lotus, and all produce national most areas.Folium Nelumbinis bitter in the mouth is pungent micro-puckery, cool in nature, and GUIXIN, liver, spleen channel, delicate fragrance rise loose; There is summer heat relieving and dampness eliminating, spleen invigorating yang invigorating, the effect of dissipating blood stasis hemostasis.Cure mainly summer-heat excessive thirst, have a headache dizzy, edema, lack of appetite abdominal distention, dysentery, leucorrhea, proctoptosis, spits blood, epistaxis, spitting of blood, has blood in stool, metrorrhagia, post partum longtime lochia, traumatic blood stasis.Modern medicine confirms that Folium Nelumbinis all has certain therapeutic effect to diarrhoea, edema, hyperlipidemia and dermatosis.
Radix Et Caulis Acanthopanacis Senticosi is dry root and rhizome or the stem of Araliaceae Radix Et Caulis Acanthopanacis Senticosi Acanthopanaz sentico.sus (Rupr.et Maxim.) Harms.Spring, Qiu Erji gather, cleaning, drying.Pungent, the micro-hardship of nature and flavor, temperature.Return spleen, kidney, heart channel.Function replenishing QI to invigorate the spleen, tonifying the kidney for tranquilization.For deficiency of both the splenic and pulmonary QI, body void is weak, inappetence, deficiency of both the lung and kidney, chronic cough dyspnea due to deficiency, the soreness of waist and knee joint of suffering from a deficiency of the kidney, deficiency of both the heart and spleen, insomnia and dreamful sleep.
Semen Pruni is the dry mature seed of rosaceous plant Prunus humilis Prunus humilis Bge., strongly fragrant Lee Prunus jaPonica Thunb. or the flat China fir 8 Prunus pedunculataMaxim. of long handle.The first two is planted to practise and claims " semen pruni humilidis et japonicae ", and rear a kind of habit claims " semen pruni tomentosae et trilobae ".Summer, the Qiu Erji mature fruit of gathering, removes sarcocarp and nucleocapsid, takes out seed, dry.Nature and flavor are pungent, bitter, sweet, flat.Return spleen, large intestine, small intestine meridian.Function loosening bowel to relieve constipation, therapeutic method to keep the adverse QI flowing downwards diuretic.For intestine-dryness due to consumption of fluid, stagnation of QI due to dyspepsia, abdominal distention constipation, edema, beriberi, dysuria.
The all medicines of Chinese medicine composition of the present invention share, and play altogether the effect of dispersing the stagnated live-QI to relieve the stagnation of QI, spleen invigorating food stagnation removing, activating blood circulation to dissipate blood stasis, dispelling phlegm and eliminating dampness.
As a preferred embodiment of the present invention, preferred described Chinese medicine composition, is made by the raw material of following weight portion: Herba Taraxaci 10-11 part, Semen Alpiniae Katsumadai 8-9 part, Radix Paeoniae Alba 6-8 part, Poria 6-7 part, Herba Menthae 5-6 part, Herba Andrographis 5-8 part, Fructus Aurantii 10-11 part, Fructus Crataegi 10-13 part, Radix Bupleuri 8-9 part, Flos Lonicerae 10-14 part, Radix Scutellariae 7-8 part, Radix Salviae Miltiorrhizae 3-4 part, Folium Nelumbinis 5-6 part, Radix Et Caulis Acanthopanacis Senticosi 5-8 part, Semen Pruni 3-4 part, Radix Glycyrrhizae 10-11 part.
Further preferably, described Chinese medicine composition, is made by the raw material of following weight portion: 10 parts of Herba Taraxacis, 8 parts of Semen Alpiniae Katsumadai, 7 parts of the Radix Paeoniae Albas, 7 parts, Poria, 6 parts of Herba Menthaes, 7 parts of Herba Andrographis, 10 parts of Fructus Aurantiis, 10 parts of Fructus Crataegis, 9 parts of Radix Bupleuri, 12 parts of Flos Loniceraes, 7 parts of Radix Scutellariaes, 3 parts of Radix Salviae Miltiorrhizaes, 5 parts, Folium Nelumbinis, 7 parts of Radix Et Caulis Acanthopanacis Senticosis, 3 parts of Semen Prunis, 10 parts, Radix Glycyrrhizae.
The method of preparing Chinese medicine composition of the present invention is as follows: get each medical material of above-mentioned components by weight percent, add the water of 5-10 times of medical material gross weight, soak 10-60 minute, decoct 1-4 time, each 1-3 hour, filters, merging filtrate, filtrate decompression is concentrated into the extractum of relative density 1.05-1.25, and this relative density is the testing result under 60 degrees Celsius, and adding ethanol is 60-90%(v/v to containing alcohol amount), standing 24 hours, get supernatant, reclaim ethanol to concentrated, obtain.The above-mentioned Chinese medicine composition of the present invention can further be prepared into conventional clinically pharmaceutical preparation, as mixture, powder, granule, tablet, capsule, is preferably mixture, tablet, capsule.
The present invention also discloses a kind of purposes of above-mentioned Chinese medicine composition, i.e. the purposes of above-mentioned Chinese medicine composition in preparation treatment or preventing canker sore medicine.Chinese medicine composition of the present invention, when treatment oral ulcer, has embodied significant antiinflammatory, antibacterial and analgesic effect, and has obtained good clinical therapeutic efficacy.The embodiment of the present invention 4 shows, Chinese medicine composition of the present invention all has good fungistatic effect to most oral cavity pathogen, and this,, for being the most easily subject to extraneous oral cavity of infecting, has very important treatment meaning.The embodiment of the present invention 5 shows, give positive drug and Chinese medicine composition of the present invention all can be obtained significant antiphlogistic effects, wherein the antiphlogistic effects of Chinese medicine composition high dose group of the present invention and positive controls have utmost point significant difference, Chinese medicine composition low dose group and positive controls have significant difference, and this shows that the high dose group of Chinese medicine composition of the present invention and the antiphlogistic effects of low dose group will significantly be better than positive controls.Mice in the embodiment of the present invention 6 licks foot response latency time showing, with model group comparison, after administration after 1h and 2h, all be greater than model group the incubation period that the mice of each administration group of Chinese medicine composition of the present invention is added foot reaction, there is dose-dependence, and be significantly greater than the incubation period of high dose group model group (
p< 0.05).Illustrate that this Chinese medicine composition of the present invention can extend adding the sufficient response latency that mice stimulates hot plate, has certain analgesic activity.Under same experiment condition, positive control drug cydiodine group (
p< 0.05) and Huanglianshangqing Tablet group (1h,
p< 0.05; 2h,
p< 0.01) also extended the incubation period that mice is added foot reaction, there is certain analgesic activity, but its analgesic effect and model group there was no significant difference.The embodiment of the present invention 7 demonstrations, Chinese medicine composition of the present invention is 100% to the clinical effective rate of oral ulcer, and wherein cure rate is 84%, and improvement rate is 16%, has certain treatment advantage.
In a word, the present invention compared with prior art, first, has the activity of fine treatment oral ulcer.Secondly, when the present invention uses as Aphthasol thing, drug effect is comparatively comprehensive, has not only embodied significant antiinflammatory bactericidal effect, and can significantly alleviate oral ulcer pain; When eliminating oral ulcer symptom, can regulate the immunocompetence of body, can make rapidly oral ulcer conditions of patients take a turn for the better, play the therapeutic effect for the treatment of both the principal and secondary aspects of a disease.And Chinese medicine composition preparation of the present invention is simple, and raw material is easy to get, and treatment cost is low, is suitable for popular use, has good application prospect.
The specific embodiment
By the specific embodiment, further describe the present invention below, but the present invention is not limited only to following examples.Within the scope of the invention or within not departing from content of the present invention, spirit and scope, Chinese medicine composition of the present invention is suitably improved, replaces the component that effect is identical, within will become apparent to those skilled in the art that they are all deemed to be included in scope of the present invention.
first, the preparation of Chinese medicine composition of the present invention
embodiment 1 Chinese medicine composition mixture of the present invention
Prescription: 10 parts of Herba Taraxacis, 8 parts of Semen Alpiniae Katsumadai, 7 parts of the Radix Paeoniae Albas, 7 parts, Poria, 6 parts of Herba Menthaes, 7 parts of Herba Andrographis, 10 parts of Fructus Aurantiis, 10 parts of Fructus Crataegis, 9 parts of Radix Bupleuri, 12 parts of Flos Loniceraes, 7 parts of Radix Scutellariaes, 3 parts of Radix Salviae Miltiorrhizaes, 5 parts, Folium Nelumbinis, 7 parts of Radix Et Caulis Acanthopanacis Senticosis, 3 parts of Semen Prunis, 10 parts, Radix Glycyrrhizae.
Preparation method: get each medical material of above-mentioned components by weight percent, add the water of 5 times of medical material gross weight, soak 45 minutes, decoct 2 times, each 2 hours, filter, merging filtrate, filtrate decompression is concentrated into the extractum of relative density 1.1, and this relative density is the testing result under 60 degrees Celsius, adding ethanol is 75%(v/v to containing alcohol amount), standing 24 hours, get supernatant, recovery ethanol is concentrated, adds water to 1000ml, stirs evenly, subpackage, flowing steam sterilization 35min, obtains mixture.
embodiment 2 Chinese medicine composition tablet of the present invention
Prescription: 10 parts of Herba Taraxacis, 8 parts of Semen Alpiniae Katsumadai, 7 parts of the Radix Paeoniae Albas, 7 parts, Poria, 6 parts of Herba Menthaes, 7 parts of Herba Andrographis, 10 parts of Fructus Aurantiis, 10 parts of Fructus Crataegis, 9 parts of Radix Bupleuri, 12 parts of Flos Loniceraes, 7 parts of Radix Scutellariaes, 3 parts of Radix Salviae Miltiorrhizaes, 5 parts, Folium Nelumbinis, 7 parts of Radix Et Caulis Acanthopanacis Senticosis, 3 parts of Semen Prunis, 10 parts, Radix Glycyrrhizae.
Preparation method: get each medical material of above-mentioned components by weight percent, add the water of 7 times of medical material gross weight, soak 60 minutes, decoct 1 time, decoct 3 hours, filter, merging filtrate, filtrate decompression is concentrated into the extractum of relative density 1.25, and this relative density is the testing result under 60 degrees Celsius, adding ethanol is 80%(v/v to containing alcohol amount), standing 24 hours, get supernatant, reclaim ethanol to concentrated, vacuum drying, granulation, tabletting and get final product.
embodiment 3 Chinese medicinal composition capsules of the present invention
Prescription: 10 parts of Herba Taraxacis, 8 parts of Semen Alpiniae Katsumadai, 7 parts of the Radix Paeoniae Albas, 7 parts, Poria, 6 parts of Herba Menthaes, 7 parts of Herba Andrographis, 10 parts of Fructus Aurantiis, 10 parts of Fructus Crataegis, 9 parts of Radix Bupleuri, 12 parts of Flos Loniceraes, 7 parts of Radix Scutellariaes, 3 parts of Radix Salviae Miltiorrhizaes, 5 parts, Folium Nelumbinis, 7 parts of Radix Et Caulis Acanthopanacis Senticosis, 3 parts of Semen Prunis, 10 parts, Radix Glycyrrhizae.
Preparation method: get each medical material of above-mentioned components by weight percent, add the water of 10 times of medical material gross weight, soak 30 minutes, decoct 4 times, each 1 hour, filter, merging filtrate, filtrate decompression is concentrated into the extractum of relative density 1.2, and this relative density is the testing result under 60 degrees Celsius, adding ethanol is 90%(v/v to containing alcohol amount), standing 24 hours, get supernatant, reclaim ethanol to concentrated, vacuum drying, granulates, encapsulated and get final product.
second portion, the pharmacodynamics of Chinese medicine composition of the present invention is investigated
the In Vitro Bacteriostasis experiment of embodiment 4 Chinese medicine compositions of the present invention
The strain that this experiment is used and culture medium are respectively staphylococcus epidermidis (32209), hemolytic hammer (32210), colon bacillus (44102), proteus vulgaris (49101), Streptococcus mutans (50001), Diplococcus pneumoniae (51252), staphylococcus aureus [26111 (5)], Candida albicans (58529-4), nutrient agar (Nat'l Pharmaceutical & Biological Products Control Institute); Opacity tube (Chinese bacterial turbidity standard, the biological combinations calibrating of Chinese medicine institute).
In Vitro Bacteriostasis experiment: 1. prepare pastille agar plate, tested material is pressed to the culture medium of doubling dilution preparation different pharmaceutical concentration, heating, modulation is required pH value separately, and 121 ℃ of sterilizing 30 min, are chilled to 45 ℃, pour into into aseptic flat board (plate d=
65 mm, 10 ml/ plates), finally make the extension rate containing tested material stock solution (2g/ml) in culture medium be respectively l:2,1:4 ... 1:1024 does blank culture medium separately simultaneously; 2. inoculation, get the bacterium in young age that reaches the third generation, with 0.9% sodium chloride solution, by standard opacity tube corrected concentrations, be respectively the bacterium liquid to be checked of 108 cfu/ml, be inoculated in respectively the agar surface of pastille separately of different doubling dilutions, inoculate parallel point 3 points simultaneously, make at every containing 2 one bacterium liquid, the liquid-drop diameter of each vaccination is 5-8 mm, after vaccination is dry, upset is dull and stereotyped cultivates 20 h observed results (simultaneously doing blank) in 36 ℃ of constant incubators; 3. criterion.Take and do not occur that the minimum dilution tested material concentration on the agar plate of bacterium colony is its minimum inhibitory concentration (MIC).
In this experiment, Chinese medicine composition is as shown in table 1 to the minimal inhibitory concentration of each pathogenic strain.
The minimal inhibitory concentration of table 1 Chinese medicine composition of the present invention to common oral cavity pathogen strain
Result demonstration, Chinese medicine composition of the present invention all has good fungistatic effect to most oral cavity pathogen, and this,, for being the most easily subject to extraneous oral cavity of infecting, has very important treatment meaning.
the antiinflammatory action of embodiment 5 Chinese medicine composition xylol of the present invention induced mice inflammatory model
Hydrocortisone has stronger anti-inflammatory activity, that synthetic is also naturally occurring glucocorticoid, hydrocortisone enters after cell, activate cytoplasmic receptor, after allosteric, enter nucleus, be combined with DNA response element, cause inhibition or the induction of genetic transcription, the expression of inflammation associated protein is changed.
, experimental technique:
40 KM mices, male and female half and half, body weight 25-30 gram, by body weight, be divided at random five groups, i.e. dosage group in model control group, compositions high dose group, compositions, compositions low dose group, positive controls, every group 8, each administration group mice gives respectively following medicine:
Compositions high dose group: gavage gives 50mg/kg embodiment 1 Chinese medicine composition, and Chinese medicine composition is by crude drug amount;
Dosage group in compositions: gavage gives 20 mg/kg embodiment 1 Chinese medicine compositions, and Chinese medicine composition is by crude drug amount;
Compositions low dose group: gavage gives 5 mg/kg embodiment 1 Chinese medicine compositions, and Chinese medicine composition is by crude drug amount;
Model control group: gavage gives the normal saline of same volume;
Positive controls: gavage gives the hydrocortisone drug solution of 5mg/kg.
Each administration group is administered once every day, successive administration 7d.After last administration, get dimethylbenzene 20 μ l, be applied to each mouse right ear.After 30 minutes, get and be respectively subject to reagent 0.03 ml to be evenly applied to respectively mouse right ear, auris dextra is put to death after 3.5 hours to dimethylbenzene, measures and respectively organizes the mice left and right ear method of double differences.Ear method of double differences assay method: place cuts two ears along auricle baseline, lays auricle with 8mm card punch at the same position of left and right ear, weighs, and the ear method of double differences=auris dextra sheet weight-left auricle weight.The ear method of double differences can be used as evaluates antiinflammatory index.
, experimental result:
Measure each experimental mice ear method of double differences and evaluate the antiphlogistic effects of each medication group medicine.Measurement result is in Table 2.
Each experimental mice ear method of double differences comparison of table 2
With model control group comparison,
* p< 0.05; With model control group comparison,
* p< 0.01;
With positive controls comparison,
# p< 0.05; With positive controls comparison,
## p< 0.01.
Result demonstration gives positive drug and Chinese medicine composition of the present invention all can be obtained significant antiphlogistic effects, wherein the antiphlogistic effects of Chinese medicine composition high dose group of the present invention and positive controls have utmost point significant difference, Chinese medicine composition low dose group and positive controls have significant difference, and this shows that the high dose group of Chinese medicine composition of the present invention and the antiphlogistic effects of low dose group will significantly be better than positive controls.
the impact of embodiment 6 Chinese medicine composition of the present invention on mice hot-plate analgesia
1 mice hot plate pain model is made
The female mice of body weight 18g-25g is placed on hot plate dolorimeter, screen 90 of qualified female mices and (lick metapedes time < 5 s or > 30 s throw aside, for preventing foot, scald, also should establish deadline, be generally 60s), be the threshold of pain index incubation period that the mice of take is added metapedes reaction, get 48 jennies, be divided into 6 groups, 8/group, it is model group, cydiodine, Huanglianshangqing Tablet group, Chinese medicine composition of the present invention (making by Chinese medicine composition prescription and preparation technology described in embodiment 1) low dose group, middle dosage group and high dose group.Each group is gastric infusion, and 1 times/day, dosage is as shown in table 3, after successive administration 14d, and the time that after mensuration last medicine, 60 min, 120 min add foot reaction.
chinese medicine composition of the present invention is on licking the impact of foot response latency in the experiment of mice hot-plate analgesia
Mice licks foot response latency time showing, with model group comparison, after administration after 1h and 2h, be all greater than model group the incubation period that the mice of each administration group of Chinese medicine composition of the present invention is added foot reaction, there is dose-dependence, and be significantly greater than the incubation period of high dose group model group (
p< 0.05).Illustrate that this Chinese medicine composition of the present invention can extend adding the sufficient response latency that mice stimulates hot plate, has certain analgesic activity.Under same experiment condition, positive control drug cydiodine group (
p< 0.05) and Huanglianshangqing Tablet group (1h,
p< 0.05; 2h,
p< 0.01) also extended the incubation period that mice is added foot reaction, there is certain analgesic activity, but its analgesic effect and model group there was no significant difference.The results are shown in Table 3.
Table 3 mice lick foot response latency timetable (
)
Note: with model group comparison, *
p< 0.05, * *
p< 0.01
the clinical therapeutic efficacy of embodiment 7 Chinese medicine composition of the present invention to hemorrhagic oral ulcer
1, clinical data
This group is observed case 40 examples, male's 23 examples wherein, women's 17 examples, age 16-49 year, 34 years old mean age.
, diagnostic criteria
There is single or a plurality of circular or oval ulcer differing in size in the mucosa that the lip in oral cavity, cheek, soft palate or gums etc. are located, ulcer surface diameter is 1～10mm, greyish white or the yellow pseudomembrane of surface coverage, concavity, clear border, around mucosa is red and micro-swollen, the local obviously causalgia in ulcer place, severe patient also can affect appetite, and lymph enlargement causes very big inconvenience to diet.
, Therapeutic Method
Embodiment 1 gained mixture for oral administration.1 dose of every day, 2 times/d, successive administration 4 days.
, criterion of therapeutical effect
Cure: in the oral cavity in ulcer surface disappear, without redness, without causalgia, lymph is normal.
Take a turn for the better: ulcer surface number reduces, ulcer surface reduced, causalgia alleviates, and lymph swells and ache and alleviates.
Invalid: ulcer surface number, ulcer surface diameter, causalgia sense are all not improved.
, curative effect
In 40 examples, cure 33 examples, account for 82.5 %; 7 examples that take a turn for the better, account for 17.5 %.
Claims (7)
1. treat a Chinese medicine composition for oral ulcer, it is characterized in that being made by the raw material of following weight portion: Herba Taraxaci 10-12 part, Semen Alpiniae Katsumadai 8-10 part, Radix Paeoniae Alba 6-9 part, Poria 6-8 part, Herba Menthae 5-7 part, Herba Andrographis 5-9 part, Fructus Aurantii 10-12 part, Fructus Crataegi 10-15 part, Radix Bupleuri 8-10 part, Flos Lonicerae 10-15 part, Radix Scutellariae 7-9 part, Radix Salviae Miltiorrhizae 3-6 part, Folium Nelumbinis 5-7 part, Radix Et Caulis Acanthopanacis Senticosi 5-9 part, Semen Pruni 3-5 part, Radix Glycyrrhizae 10-12 part.
2. the Chinese medicine composition for the treatment of oral ulcer as claimed in claim 1, is characterized in that being made by the raw material of following weight portion: Herba Taraxaci 10-11 part, Semen Alpiniae Katsumadai 8-9 part, Radix Paeoniae Alba 6-8 part, Poria 6-7 part, Herba Menthae 5-6 part, Herba Andrographis 5-8 part, Fructus Aurantii 10-11 part, Fructus Crataegi 10-13 part, Radix Bupleuri 8-9 part, Flos Lonicerae 10-14 part, Radix Scutellariae 7-8 part, Radix Salviae Miltiorrhizae 3-4 part, Folium Nelumbinis 5-6 part, Radix Et Caulis Acanthopanacis Senticosi 5-8 part, Semen Pruni 3-4 part, Radix Glycyrrhizae 10-11 part.
3. the Chinese medicine composition for the treatment of oral ulcer as claimed in claim 2, is characterized in that being made by the raw material of following weight portion: 10 parts of Herba Taraxacis, 8 parts of Semen Alpiniae Katsumadai, 7 parts of the Radix Paeoniae Albas, 7 parts, Poria, 6 parts of Herba Menthaes, 7 parts of Herba Andrographis, 10 parts of Fructus Aurantiis, 10 parts of Fructus Crataegis, 9 parts of Radix Bupleuri, 12 parts of Flos Loniceraes, 7 parts of Radix Scutellariaes, 3 parts of Radix Salviae Miltiorrhizaes, 5 parts, Folium Nelumbinis, 7 parts of Radix Et Caulis Acanthopanacis Senticosis, 3 parts of Semen Prunis, 10 parts, Radix Glycyrrhizae.
4. the Chinese medicine composition of the treatment oral ulcer as described in claim 1,2 or 3, is characterized in that: described Chinese medicine composition is mixture, tablet or capsule.
5. a method of preparing the Chinese medicine composition of the treatment oral ulcer as described in claim 1,2 or 3, it is characterized in that comprising the following steps: get each medical material, the water of 5-10 times that adds medical material gross weight, soak 10-60 minute, decoct 1-4 time, each 1-3 hour, filter, merging filtrate, filtrate decompression is concentrated into the extractum of relative density 1.05-1.25, this relative density is the testing result under 60 degrees Celsius, add ethanol to being 60-90%v/v containing alcohol amount, standing 24 hours, get supernatant, reclaim ethanol to concentrated, obtain.
6. the purposes of the Chinese medicine composition of the treatment oral ulcer as described in claim 1,2 or 3 in preparation treatment or preventing canker sore medicine.
7. purposes as claimed in claim 6, is characterized in that: described Chinese medicine composition has antiinflammatory, antibacterial and analgesic activity.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104547689A (en) * | 2014-12-31 | 2015-04-29 | 曾子维 | Application of medicine composition in preparing medicine curing oral ulcer |
| CN105125994A (en) * | 2015-09-16 | 2015-12-09 | 程俊 | Traditional Chinese medicine for treating oral ulcer |
| CN105147976A (en) * | 2015-10-13 | 2015-12-16 | 青岛云天生物技术有限公司 | Traditional Chinese medicine composition for treating combination of acute laryngopharyngitis and oral ulcer |
| CN105194491A (en) * | 2015-09-21 | 2015-12-30 | 宋晓梅 | Traditional Chinese medicine composition for treating oral ulcers |
| CN106309906A (en) * | 2016-11-11 | 2017-01-11 | 东莞市百替生物科技有限公司 | Traditional Chinese medicine composition for treating dental ulcer and preparation method |
| CN107173763A (en) * | 2017-06-13 | 2017-09-19 | 辽宁中医药大学 | It is a kind of to treat Chinese herbal medicine jelly of recurrent oral ulceration and preparation method thereof |
-
2014
- 2014-08-12 CN CN201410394180.6A patent/CN104096154A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104547689A (en) * | 2014-12-31 | 2015-04-29 | 曾子维 | Application of medicine composition in preparing medicine curing oral ulcer |
| CN105125994A (en) * | 2015-09-16 | 2015-12-09 | 程俊 | Traditional Chinese medicine for treating oral ulcer |
| CN105194491A (en) * | 2015-09-21 | 2015-12-30 | 宋晓梅 | Traditional Chinese medicine composition for treating oral ulcers |
| CN105147976A (en) * | 2015-10-13 | 2015-12-16 | 青岛云天生物技术有限公司 | Traditional Chinese medicine composition for treating combination of acute laryngopharyngitis and oral ulcer |
| CN106309906A (en) * | 2016-11-11 | 2017-01-11 | 东莞市百替生物科技有限公司 | Traditional Chinese medicine composition for treating dental ulcer and preparation method |
| CN107173763A (en) * | 2017-06-13 | 2017-09-19 | 辽宁中医药大学 | It is a kind of to treat Chinese herbal medicine jelly of recurrent oral ulceration and preparation method thereof |
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