CN104093850B - 用于减少非特异性核酸扩增的方法和试剂盒 - Google Patents

用于减少非特异性核酸扩增的方法和试剂盒 Download PDF

Info

Publication number
CN104093850B
CN104093850B CN201380009386.6A CN201380009386A CN104093850B CN 104093850 B CN104093850 B CN 104093850B CN 201380009386 A CN201380009386 A CN 201380009386A CN 104093850 B CN104093850 B CN 104093850B
Authority
CN
China
Prior art keywords
nucleic acid
primer
amplification
nucleotide
atn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201380009386.6A
Other languages
English (en)
Chinese (zh)
Other versions
CN104093850A (zh
Inventor
J.R.纳尔逊
G.A.格罗斯曼
R.S.杜蒂
S.J.莎
R.C.赫勒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare UK Ltd
Global Life Sciences Solutions Operations UK Ltd
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Electric Co filed Critical General Electric Co
Publication of CN104093850A publication Critical patent/CN104093850A/zh
Application granted granted Critical
Publication of CN104093850B publication Critical patent/CN104093850B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201380009386.6A 2012-02-15 2013-02-14 用于减少非特异性核酸扩增的方法和试剂盒 Active CN104093850B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201261599119P 2012-02-15 2012-02-15
US61/599,119 2012-02-15
US61/599119 2012-02-15
US13/446,474 US9353393B2 (en) 2012-02-15 2012-04-13 Methods and kits for reducing non-specific nucleic acid amplification
US13/446474 2012-04-13
US13/446,474 2012-04-13
PCT/US2013/026136 WO2013123187A1 (en) 2012-02-15 2013-02-14 Methods and kits for reducing non-specific nucleic acid amplification

Publications (2)

Publication Number Publication Date
CN104093850A CN104093850A (zh) 2014-10-08
CN104093850B true CN104093850B (zh) 2016-12-07

Family

ID=48945882

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380009386.6A Active CN104093850B (zh) 2012-02-15 2013-02-14 用于减少非特异性核酸扩增的方法和试剂盒

Country Status (6)

Country Link
US (1) US9353393B2 (enExample)
EP (1) EP2814976B1 (enExample)
JP (1) JP6225123B2 (enExample)
CN (1) CN104093850B (enExample)
CA (1) CA2864676C (enExample)
WO (1) WO2013123187A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014018093A1 (en) 2012-07-26 2014-01-30 Illumina, Inc. Compositions and methods for the amplification of nucleic acids
US9938568B2 (en) 2013-07-26 2018-04-10 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9587263B2 (en) * 2014-03-26 2017-03-07 General Electric Company Isothermal amplification under low salt condition
US10683534B2 (en) * 2015-01-27 2020-06-16 BioSpyder Technologies, Inc. Ligation assays in liquid phase
US11987836B2 (en) 2014-09-30 2024-05-21 Global Life Sciences Solutions Usa Llc Method for nucleic acid analysis directly from an unpurified biological sample
ES2912965T3 (es) 2015-09-09 2022-05-30 Drawbridge Health Inc Dispositivos para la recopilación, estabilización y conservación de muestras
US10077459B2 (en) * 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product
WO2018209092A1 (en) * 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
US11001868B2 (en) * 2017-08-11 2021-05-11 Global Life Sciences Solutions Operations UK Ltd Cell-free protein expression using double-stranded concatameric DNA
CN111334562A (zh) * 2018-12-19 2020-06-26 合肥铼科生物科技有限公司 一种含有修饰引物的核酸扩增方法和试剂盒
CN110295241A (zh) * 2019-07-11 2019-10-01 深圳易致生物科技有限公司 用于检测尿路感染的引物组和包含该引物组的试剂盒
FR3110602B1 (fr) 2020-05-20 2025-03-07 Anova Plus Methode de detection sur le terrain de la presence et de la mutliresistance d’un bio-agresseur, notamment dezymo-septoria tritici,dans des culturescerealieres
CN112899346A (zh) * 2021-01-05 2021-06-04 南京普济生物有限公司 一种降低pcr非特异性扩增的核苷酸及其设计方法与应用
FR3129407B1 (fr) 2021-11-25 2024-12-13 Anova Plus Methode de detection de la presence d’un pathogene et determination de ses caracteristiques en sante humaine et animale
CN114277103A (zh) * 2022-01-21 2022-04-05 杭州飞时达生物科技有限公司 一种基于六聚体随机引物高效滚环扩增方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002042490A1 (en) * 2000-11-24 2002-05-30 Asper OÜ Method of optimising the sequences of synthetic nucleic acids

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040086880A1 (en) * 1999-07-20 2004-05-06 Sampson Jeffrey R Method of producing nucleic acid molecules with reduced secondary structure
US7993839B2 (en) * 2001-01-19 2011-08-09 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US20020197618A1 (en) * 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US20030165859A1 (en) * 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US7176002B2 (en) 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
EP1426448A1 (en) 2002-12-03 2004-06-09 bioMérieux BV Method for lowering the effects of sequence variations in a diagnostic hybridization assay, probe for use in the assay and assay
US8871469B1 (en) 2004-11-13 2014-10-28 Steven Albert Benner Self-avoiding molecular recognition systems in DNA priming
DK1885880T3 (da) 2005-04-29 2010-11-08 Synthetic Genomics Inc Amplifikation og kloning af enkelte DNA-molekyler ved anvendelse af rolling circle-amplifikation
DE602007013223D1 (de) 2006-06-01 2011-04-28 Trilink Biotechnologies San Diego Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
ES2647237T3 (es) 2007-07-03 2017-12-20 Genaphora Ltd. Cebadores quiméricos para reacciones de amplificación de ácidos nucleicos mejoradas
WO2009046149A1 (en) 2007-10-01 2009-04-09 Applied Biosystems Inc. Chase ligation sequencing
JP2013507910A (ja) 2009-10-13 2013-03-07 シンテッツァ モレキュラー ディテクション イスラエル リミテッド 核酸試料から標的配列を増幅するための方法および組成物
US20110195457A1 (en) 2010-02-09 2011-08-11 General Electric Company Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002042490A1 (en) * 2000-11-24 2002-05-30 Asper OÜ Method of optimising the sequences of synthetic nucleic acids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Design considerations and effects of LNA in PCR primers;Latorra D.等;《Molecular and Cellular Probes》;20031001;第17卷(第5期);第253-259页 *
Isothermal strand-displacement amplification applications for high-throughput genomics;Detter J.C.等;《Genomics》;20021231;第80卷(第6期);第691-698页 *

Also Published As

Publication number Publication date
WO2013123187A1 (en) 2013-08-22
US20130210078A1 (en) 2013-08-15
CA2864676C (en) 2021-06-15
EP2814976B1 (en) 2017-10-18
JP2015507930A (ja) 2015-03-16
CN104093850A (zh) 2014-10-08
JP6225123B2 (ja) 2017-11-01
EP2814976A1 (en) 2014-12-24
US9353393B2 (en) 2016-05-31
CA2864676A1 (en) 2013-08-22
EP2814976A4 (en) 2015-09-09

Similar Documents

Publication Publication Date Title
CN104093850B (zh) 用于减少非特异性核酸扩增的方法和试剂盒
JP6574178B2 (ja) リガーゼ支援核酸環状化及び増幅
AU2011253427B2 (en) Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers
CN106715706B (zh) 直接从未纯化的生物样本分析核酸的方法
CN106460052B (zh) 双链核酸的合成
AU2013203624B2 (en) Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers
EP3122888B1 (en) Isothermal amplification under low salt condition
JP6847364B2 (ja) オリゴカチオン複合プライマー配列を使用する等温増幅
JP2022002538A (ja) リガーゼ支援核酸環状化および増幅
US9512472B2 (en) Method of amplifying nucleic acid sequences
KR101922124B1 (ko) 시료 중 ｒｎａ로부터 ｄｎａ를 증폭하는 방법
WO2024073034A1 (en) Simplified sequencing library preparation for dna

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20210913

Address after: England

Patentee after: Global life science solutions UK Ltd.

Address before: Buckinghamshire

Patentee before: GE HEALTHCARE UK Ltd.

Effective date of registration: 20210913

Address after: Buckinghamshire

Patentee after: GE HEALTHCARE UK Ltd.

Address before: New York State, USA

Patentee before: General Electric Co.