CN104087607A - 编码嵌合抗原受体蛋白的核酸及表达嵌合抗原受体蛋白的t淋巴细胞 - Google Patents
编码嵌合抗原受体蛋白的核酸及表达嵌合抗原受体蛋白的t淋巴细胞 Download PDFInfo
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Abstract
Description
嵌合抗原受体 | 胞外结合区-跨膜区-胞内信号区1-胞内信号区2etc | 描述 |
806-δZ | scFv(EGFR)-CD8-CD3δzeta | 阴性对照 |
806-Z | scFv(EGFR)-CD8-CD3zeta | 第一代 |
806-BBZ | scFv(EGFR)-CD8-CD137-CD3zeta | 第二代 |
806-28Z | scFv(EGFR)-CD28-CD28-CD3zeta | 第二代 |
806-28BBZ | scFv(EGFR)-CD28-CD28-CD137-CD3zeta | 第三代 |
转染有下列CAR的CD8+T淋巴细胞 | CD8+T淋巴细胞eGFP阳性率 |
806-δZ | 38.8% |
806-Z | 62% |
806-BBZ | 45% |
806-28Z | 78% |
806-28BBZ | 33.8% |
细胞名称 | 来源 | 性质 |
U87 | ATCC HTB-14 | 低表达EGFR |
U87-EGFRvIII | 本实验自行构建和保种 | 过表达EGFRvIII |
U87-EGFR | 本实验室自行构建和保种 | 过表达EGFR |
PANC-1 | ATCC CRL-1469 | 过表达EGFR |
CFPAC-1 | ATCC CRL-1918 | 过表达EGFR |
BxPC-3 | ATCC CRL-1687 | 过表达EGFR |
Claims (11)
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