CN104083427B - Prevent and treat the compound Radix Astragali polysaccharide particle of bird flu - Google Patents
Prevent and treat the compound Radix Astragali polysaccharide particle of bird flu Download PDFInfo
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Abstract
Compound Radix Astragali polysaccharide particle the present invention relates to prevent and treat bird flu, can effectively solve the problems, such as bird flu medication, and its technical scheme for solving is to be by following weight meter：75 85g of the 650g of astragalus polyose 550, the 220g of Inokopolyose 180, the 130g of ram's horn grass polysaccharide 110 and semen momordicae frost make material medicine, the auxiliary material for adding material medicine weight 3% again is prepared into particle, described auxiliary material is starch, Icing Sugar, one or more mixing of dextrin, abundant raw material of the present invention, cheap, drug safety has no toxic side effect, using effect is good, is the innovation on the herbal medicine thing for prevent and treat bird flu.
Description
Technical field
The present invention relates to medicine, particularly a kind of compound Radix Astragali polysaccharide particle for preventing and treating bird flu.
Background technology
The object of avian flu virus infection is mainly birds, but in recent years, especially H5N1 highly pathogenic bird flus are sick
Poison can cause various mammalian infections with it is dead, such as betide in China zoo tiger, leopard fowl stream in tiger and Thailand zoo
The infection morbidity of sense, causes mortality, other animals such as infection morbidity of pig, cat, dog etc. with it is dead also when have been reported that.When
So, in the avian flu virus infection of many mammals, the harm with the infection of pig bird flu to the mankind is maximum.Once people
After influenza virus and avian influenza virus co-infection pig, it is likely that produce and propagated between people and popular variant.Research is recognized
For 1957 to 1968 years H2N2 subtype virus of Asia prevalence, its HA, NA and PB1 gene all comes from avian flu
Poison, other genes remain the gene of the H1N1 viruses being popular at that time in crowd；The H3N2 of Hong Kong pandemic is sub- within 1968
Type virus, its HA and PB1 also comes from avian influenza virus, and other genes come from the H2N2 diseases being popular at that time in crowd
Poison.Cause the pandemic virus of human influenza twice, be all the hybrid virus of people source strain and fowl source strain.Because pig is this
Played an important role in the forming process of hybrid virus, it is considered to be " blender ".In addition, avian flu virus infection mammal
Afterwards, in vivo after adaptation after a while, its receptor-specific may change, it is also possible to become that the mankind can be infected
Strain.
From the point of view of the infection of current mammal with incidence, the animal such as tiger, leopard, cat in cats and Mustelidae are dynamic
Ermine class animal such as ferret, mink, stone marten etc. in thing is very sensitive to avian influenza virus, and ferret is also the real ideal of influenza virus
Test animal.Critical role and China with reference to mink in China's special economic animal cultivation constantly have highly pathogenic bird flu
The reality of generation, reminds our generations on mink bird flu to have to give the attention of height with influence.
Heredity and variation are any biological fundamental characteristics；Especially under poor environment pressure, it can be more promoted to become
Different, the pressure of such as antibody can cause the change of virus antigenicity.In China, using H9N2 subtype avian influenzas vaccine at least
The history of 10 years, and at least 3 groups currently a popular of H9 subtype avian influenza virus different types of antigen group, different antigen groups it
Between cross reactivity it is poor；And the application of H5 subtype influenza vaccines also has certain hour, according to existing report data, China is wide
All there is larger difference, and animal in the highly pathogenic bird flu haemagglutinin antigen on the ground such as west, Yunnan, Jiangxi and Hunan
Experiment shows, to the virus of duck inoculation, what the later stage was discharged viral just has quantity not grade amino acid with former virus inoculation compared with
Difference, absolutely proved H5 subtype avian influenza virus antigens be susceptible to variation, and avian influenza virus antigen variation seem into
An active period is entered.Therefore, the change of avian influenza virus antigen, is either still immunized prevention and control to animal to human vaccination
All cause very big difficulty.
The protective agents for bird flu lack at present：The Western medicine such as amantadine treat the medicine of influenza as people and livestock and poultry
Decades are used.But, H3N1, H3N2 of people have generated the strain of anti-amantadine.There is media report recently,
Someone is also had begun to produce new strain with " Tamiflu ", and this is that we are concerned about very much, but this mainly appears on the body of people
On.It is antiviral for Western medicine not allow to use for preventing and treating bird flu China.According to existing veterinary drug residues regulations, as long as
It was found that the Western medicine such as amantadine antiviral agent being used for veterinary drug sale and being produced it is necessary to give administrative penalty.So, at present anti-
The bird flu aspect for controlling birds there is no antiviral veterinary drug.
National regulation is violated at part plant family to be still used to prevent and treat bird flu using the Western medicine such as amantadine antiviral agent：①
Due to destroying the immune defense system of body after birds infection bird flu first, cause the resistance of body and itself repair ability
Lose；2. Western medicine antiviral agent has partial antagonism to virion after being used for clinic, but for the body cell of birds
Similarly toxic side effect, so, Western medicine antiviral drugs is used for the avian influenza prevention effect of birds not on veterinary clinic
It is preferable；3. the serious problems such as food security can be caused after in the feeding process of birds using Western medicine antiviral drugs, so that shadow
Sound has arrived the prevention and control safety of the healthy and human diseases of the mankind.In clinical prevention research of the mankind for the bird flu of birds
With in exploration find part herbal medicine kind such as Radix Astragali, semen momordicae etc. for prevent and treat birds bird flu clinical effectiveness even not
Mistake, and research also found that the herbal medicines such as Radix Astragali not only have stronger clinical antiviral ability, while also having enhancing body
Immunity and premunition, improve the repair ability of body itself.Traditional herbal medicine is fairly simple due to processing technology, relative to add
Work is coarse, directly admixed after being boiled with simple meal or fine powder or tradition more bird feed or drinking-water in, herbal medicine it is effective
Composition cannot get it is sufficiently effective absorb, cause clinical application effect not ideal.Therefore, on the medicine of preventing and treating bird flu
Improvement be current urgent need to resolve problem.
The content of the invention
For above-mentioned situation, to solve the defect of prior art, the purpose of the present invention is just to provide a kind of preventing and treating bird flu
Compound Radix Astragali polysaccharide particle, can effectively solve the problems, such as bird flu medication.
The technical scheme that the present invention is solved is to be by following weight meter：Astragalus polyose 550-650g, Inokopolyose 180-
220g, ram's horn grass polysaccharide 110-130g and semen momordicae frost 75-85g make material medicine, then the auxiliary material for adding material medicine weight 3%
Particle is prepared into, described astragalus polyose is the dry Radix Astragali, is added water, and is extracted three times, is extracted for the first time, first with 12 times of weights of the Radix Astragali
The water immersion 30-60min of amount, is fried boiling, then is cooked by slow fire 1.5 hours, filters, and obtains the first filtrate, and the dregs of a decoction add 10 times of weight of the Radix Astragali
Water, carry out second extraction, boil, then be cooked by slow fire 1.5 hours, filter, obtain the second filtrate, the dregs of a decoction add 10 times of weight of the Radix Astragali again
Water, carry out the third leaching, boil, then be cooked by slow fire 1 hour, filter, obtain the 3rd filtrate, merge three filtrates, be concentrated into 1：1
Concentrate, precipitated with the absolute ethyl alcohol of 4 times of volumes of concentrate, drying precipitate obtains astragalus polyose；Described Inokopolyose
It is, by the dry root of bidentate achyranthes, to add water, solid-liquid ratio 1:15,100 DEG C of extraction 2h, leaching liquor adds 5 times of mass concentrations of weight 95% of the root of bidentate achyranthes
Ethanol, extract 2 times, 2 hours every time, merging filtrate, concentration, dry, obtain Inokopolyose；Described ram's horn grass polysaccharide is to incite somebody to action
Radix ranunculi ternati adds water, and extracts 2 times, and each amount of water is 12 times of radix ranunculi ternati weight, is extracted 3 hours every time, merges leaching liquor twice,
Be concentrated into equivalent to the concentrate containing crude drug 800g/L, plus the volume of concentrate 50% absolute ethyl alcohol, put only 6 hours, discard top
Liquid, obtains sediment, as drying precipitate, ram's horn grass polysaccharide；Described semen momordicae frost is, by Semen Momordicae, to load squash type
Making drugs into frostlike powder machine is suppressed, and controls temperature for 100 DEG C, when oil content reaches 17-20%, stops squeezing, and the dregs of a decoction are pulverized and mixed uniformly, is obtained
Semen momordicae frost；Described auxiliary material is starch, Icing Sugar, one or more mixing of dextrin.
Abundant raw material of the present invention, cheap, drug safety, has no toxic side effect, and using effect is good, is preventing and treating bird flu
Innovation on herbal medicine thing.
Specific embodiment
Specific embodiment of the invention is described in further detail with reference to embodiments.
Embodiment 1
The present invention in the specific implementation, can be by following weight meter：Astragalus polyose 550g, Inokopolyose 180g, radix ranunculi ternati
Polysaccharide 110g and semen momordicae frost 75g make material medicine, and the auxiliary material of addition material medicine weight 3% is prepared into particle, described auxiliary material
It is starch, Icing Sugar, dextrin, by weight 1：3：1 mixture is uniformly formed.
Embodiment 2
The present invention in the specific implementation, can be by following weight meter：Astragalus polyose 600g, Inokopolyose 200g, radix ranunculi ternati
Polysaccharide 120g and semen momordicae frost 80g make material medicine, and the auxiliary material of addition material medicine weight 3% is prepared into particle, described auxiliary material
It is starch, Icing Sugar, by weight starch：Icing Sugar=1：3 mixings are formed.
Embodiment 3
The present invention in the specific implementation, can also be by following weight meter：Astragalus polyose 650g, Inokopolyose 220g, ram's horn
Grass polysaccharide 130g and semen momordicae frost 85g make material medicine, and the auxiliary material of addition material medicine weight 3% is prepared into particle, and described is auxiliary
Expect to be starch, dextrin, by weight 1：1 mixture is uniformly formed.
In the invention described above prescription,
The Radix Astragali：It is legume astragalus mongolicusAstragalus memeranaceus (Fisch.) Bge . var
.mongholicus (Bge.) Hsiao or Astragalus membranacusA . membranaceus (Fisch.) root of Bge..Main product in
The ground such as the Inner Mongol, Shanxi, Heilungkiang.
The property of medicine：It is sweet, tepor.Returns spleen, lung channel.Effect：Air making-up and spleen enlivening, yang invigorating, benefit defends solid table, inducing diuresis to remove edema, detoxification
Myogenic.
Modern study：Mainly containing glycoside, polysaccharide, flavones, amino acid, trace element etc..The Radix Astragali can promote organism metabolism, resist
Fatigue, the renewal for promoting serum and hepatic protein；There are obvious diuresis, Jishengshenqiwan Urine proteins can be eliminated；Can change
Kind anaemia animal phenomenon；Hypoglycemia can be raised；Can excited breathing；Can strengthen and regulation body's immunity, to interferon system
Having facilitation can improve the premunition of body；Various viral institute's cytopathogenic effects such as infected by influenza have slight inhibitory action,
Influenza virus infected has protective effect；There is wide antibacterial action；In milkvetch seed cell culture, cell number can be made
Showed increased, cell growth is vigorous, life；Myocardial contractive power can be strengthened, protect cardiovascular system, anti-arrhythmia to expand
Coronary artery and peripheral vascular are opened, is reduced blood pressure, thrombocyte adhesiveness power can be reduced, reduce thrombosis；Also reducing blood lipid, anti-ageing
Always, the effect such as anti anoxia, radioresistance, liver protection.It is many containing polysaccharide, saponin, flavones, amino acid etc. in the research discovery Radix Astragali in recent years
Active ingredient is planted, these active components play the role of to promote antibody tormation and immune response, wherein with to astragalus polyose
（Astragalus Polysacharin, APS）Research be reported as it is many.Astragalus polyose is one of main active of the Radix Astragali,
By high-tech extraction, it is isolated.Astragalus polyose is the main component that the Radix Astragali plays a role.
The root of bidentate achyranthes：It is the amaranthaceous plant root of bidentate achyranthes（Radix achyranthis bidentatae）Achyranthes bidentataAnd radix cyathulae Bl.（RADIX CYATHULAE）Cyathula officinalisThe root of Kuan.
The property of medicine：It is bitter, sweet, sour, flat.Return liver and kidney channel.Effect：Invigorate blood circulation, filling liver kidney, strengthening the bones and muscles, promoting diuresis for stranguria, ignite
（Blood）It is descending.
Modern study：The root of bidentate achyranthes contains triterpenoid saponin（Turn into oleanolic acid and sugar after hydrolysis）, ecdysterone, root of bidentate achyranthes steroid
The Analysis of Steroids such as ketone, rubrosterone and polysaccharide constituents.Additionally, the root of bidentate achyranthes also containing 12 kinds of amino acid such as arginine and
The trace element such as the compounds such as alkaloids, Coumarins and iron, copper.Root of bidentate achyranthes total saposins have obvious excitement to uterine smooth muscle
Effect, radix achyranthis bidentatae benzene extractives plays the role of obvious antifertility, anti-implantation and Robust speaker feature, and the active ingredient of antifertility is to cast off a skin
Sterol.Root of bidentate achyranthes alcohol extract has inhibitory action to experiment toy heart, and decoction also has inhibitory action to Myocardium in Anaesthetized Dogs.Decoction and
Alcohol extract has of short duration step-down and mild diuretic effect, and is acted on respiratory stimulant.Radix achyranthis bidentatae can reduce rat whole blood viscosity,
Hematocrit, erythrocyte aggregation index, and have anticoagulation.Ecdysterone has effect for reducing fat, and can substantially reduce blood sugar.Ox
Knee has anti-inflammatory, analgesic activity, can improve body's immunity.
Radix ranunculi ternati：It is ranunculaceae plant Seynuns KugelannRununculus ternatusThunb. root tuber.
The property of medicine：It is sweet, pungent, tepor.Return liver, lung channel.Effect：Reducing phlegm and resolving masses, removing toxicity for detumescence.
Modern study：Radix ranunculi ternati lactone containing Seynuns Kugelann, protoanemonin, arachidic acid, myristic acid octadecyl ester, beans steroid
Alcohol, cupreol, glucose, arabinose, galactolipin, also containing oils and vegetable soda.
1. anti-tubercle bacillus and other bacteriums：The anti-tubercle bacillus mechanism of radix ranunculi ternati, someone is by carrying out trace element point to it
Think after analysis, obvious, in the radix ranunculi ternati higher Zn lower than normal person of tubercular's trace elements in scalp hair Zn, Fe, Mn contents
(40.1), Fe (1306.4), Mn (74.6) content be counteracted that because human body immunocyte suppresses caused by Zn reductions, so as to weaken
It is to the neurological susceptibility for infecting but the reasons why this sterilization mechanism and insufficient.Zhan Li etc. is using reverse transcriptional PCR half
Quantitative (QC-RT PCR) method and PCR method, active ingredient ranunculus ternatus lactone (Tern) to radix ranunculi ternati have carried out anti-tubercle bacillus
The research of molecular immunology mechanism, as a result shows that Tern may inducing peripheral blood vessel GLS high level gene expressions, killing intracellular cause
Germ MTB, and in dose dependent, to resistance with untreated lunger's induced levels without significant difference.Further
Research shows, Tern may be by promoting the expression of GLSmRNA, the sterilized energy of enhancing body cell toxic T lymphocyte (CTL)
Power, so as to reach the effect that treating tuberculosis dormancy falls, is also possible to by reducing the small hot body gram egg calabash shell serving as a dippers of tuberculosis dormancy 16KDa in addition
The expression of (16KDaSHSP) gene, promotes GLS high level expressions, enhancing body CTL sterilization energy while dormancy bacterium is activated
Power, the effect H for reaching antimicrobial agent draws and thanks and builds equal utilization bidirectional electrophoresis technique, it is found that cat's claw P.E acts on the knot of shield
In enterprise's cell egg mortar expression map of core mycobacteria clinical separation strain, the concentration for having 22 protein spots has difference, profit
Cupboard, wherein Jue glands is divided to seek methionine synthetase, hinder with ground substance assistant laser gill suction/ionization time of flight mass spectrometry technology | diindyl 1
One phosphoglycerol synthase, the CoA hydrases of alkene acyl one, succinic thiokinase expression are substantially lowered, and its research shows, cat melon
Careless extract acts on multiple targets of a plurality of approach of tulase, and this locust approach includes that amino acid is metabolized and the polyamines of correlation is closed
Walk on tiptoe and draw from matter secretion, transport and immune molecular chaperones n into, fatty acid metabolism, central metabolism approach and may participating in, this with
The multicomponent property canopy symbol of Chinese medical extract.Upper in application, it is right in examination is alert that cat melon grass leopard dips in sanction, crude drug powder and alcohol extract forever
Strong poison enters to step on tuberculosis friendly (H37RV) the decoction and the Mlc of crude drug powder of different degrees of inhibitory action radix ranunculi ternatis
It is 1：10, the Mlc of its alcohol extract is 1：1000, and bacteriostasis is slightly strong compared with isoniazid." by radix ranunculi ternati, blue or green punt-pole infusion
With 1 after filtering：200 concentration are made improvement Roche Chinese-medicinal culture medium and control medium, it is sterilized after point shave to connect and release tuberculosis lush and mix
Suspension, placement is cultivated in giving 37 DEG C of permanent waterfall incubators, tulase well-grown in network fruit control medium, and two Chinese-medicinal culture mediums are without knot
Pyrenomycetes growth radix ranunculi ternatis Aqueous extracts have suppression to staphylococcus aureus, staphylococcus albus, four streptococcus, shigella dysenteriae etc.
Make and use, and drug resistant M bacillus can be suppressed, Yue Shi mouse cancers bacillus is resistant to, is reduced protozoan infection rate.
2. antitumor research
Radix ranunculi ternati parenteral solution has significant inhibitory action to the swollen lame strain such as mouse S180, $ 37, EAC.It is relevant that its is antineoplastic
Mechanism, once someone thought that Cu/Zn values were raised in cancer patient's serum from the angle of trace elements and health, and radix ranunculi ternati Cu/Zn
Value tends to opposite with cancer patient Cu/Zn, it is thus possible to helps and gives Cu, Zn balance in regulation patient's body and play anticancer effect.
3. antiinflammatory action
The anti-inflammation effect of compound radix ranunculi ternati water extract is notable, and reason may be by suppressing the expansion of capillary
, distant permeability is reduced, reduce diffusate and drawn with reaching antiinflammatory action ∞, both grain hair shell sheet is used to treat luxuriant and rich with fragrance Tuberculous radix ranunculi ternati
Lymphnoditis.
4. protectiveness inhibitory action
Research thinks that radix ranunculi ternati has different degrees of suppression to animal central nervous systems, heart, respiratory system and intestinal wall
Make and use, and the momentary decline of blood pressure can be made, to blood vessel but without dilating effect, infer that the medicine may play protectiveness suppression to human body
Effect, is conducive to improving constitution and builds up one's resistance to disease.
Semen momordicae：For cucurbitaceous plant Momordica cochinchiensis Momordica cochinchinensis (Lour.) Spreng. into
Ripe seed.
The property of medicine：Bitter, micro-sweet, it is cool.It is poisonous.Return liver, spleen, stomach.Effect：Attack poison and treat sore, dispersing swelling and dissipating binds.
Modern study：Containing momordin, momordic acid, Cochinchinin, oleanolic acid, sterol, amino acid, and oil
35.72%th, protein 30.59%, trehalose etc..Momordin has anti-inflammatory and a hypotensive activity, and can suppress isolated frog heart and
In vitro rabbit duodenum.Infusion alcohol-waters leachate and alcohol leaching liquid, test in dog,CatAndRabbitDeng anesthetized animal, have
Antihypertensive effect.But toxicity is larger, no matter vein or intramuscular injection, animal is in dead in a few days.Rat intravenous injection semen momordicae
Saponin, blood pressure temporarily declines, and breathes of short duration excitement, and heartbeat is accelerated.Being injected in Canine femoral artery can be temporarily increased lower extremity blood flow amount, its
Action intensity is about the 1/8 of papaverine.Inhibitory action is to isolated frog heart and in vitro rabbit duodenum, and to GPI then
Effect, the effect of antagonism papaverine of acetylcholine can be strengthened, during high concentration and cause irreversibility to be shunk.Oral Administration in Rats or skin
Lower injection ECMS, can significantly inhibit the foot smell of urine edema that carrageenan causes.There is haemocylolysis to rabbit erythrocyte.Seed
Saponin：6.6mg/kg can cause of short duration respiratory stimulant and drop in blood pressure to rat intravenous injection, and can increase dog hind leg peripheral blood
The blood flow of pipe, has inhibitory action to isolated frog heart；2×10（- 4) g/ml concentration can increase excitement of the acetylcholine to GPI
Effect；The rat red swollen feet inflammation that gavage or hypodermic injection Carrageenan cause has inhibitory action；There is haemocylolysis；To small
The median lethal dose intravenous injection of mouse is 32.35mg/kg, and intraperitoneal injection is 37.34mg/kg.Momordicae semen extract to anesthesia dog,
Cat and rabbit etc. have antihypertensive effect, and rat intravenous injection ECMS can be such that blood pressure temporarily declines, and heartbeat is accelerated, and breathe of short duration
It is excited.Being injected in Canine femoral artery can be temporarily increased lower extremity blood flow amount, and its action intensity is about the 1/8 of papaverine.Have in addition anti-
Scorching and haemocylolysis.
Semen momordicae fatty oil content is determined：Separately sampled product meal 1g, it is accurately weighed, wrapped with filter paper, put surname extraction
In device, add ether 80ml that extraction element is installed, the h of heating water bath refluxing extraction 8, to defecation fat oil, collects extract solution and puts perseverance
Again in good evaporating dish, low temperature volatilizes ether in water-bath, puts in Constant Temp. Oven, 100 DEG C of h of drying 1, takes out dislocation
In drier, 30 min are cooled down, it is accurately weighed, calculate, fat oil average content is 40. 74% in drawing semen momordicae health product, wood
Soft-shelled turtle frost fat oil average content 17.13%, averagely goes oil cut rate 19.80%.Present invention control semen momordicae frost is containing 17~20% fat
Oil, there is certain reason.
Instant component uses astragalus polyose, Inokopolyose, ram's horn grass polysaccharide and semen momordicae frost science, reasonable compatibility, mutually
Mutually support, effective for treatment bird flu, good effect, drug safety, and obtained fully proving through experiment, relevant testing data
It is as follows：
First, astragalus polyose pharmacological activity test：
1.1 test materials
Astragalus polyose of the present invention.
1.2 testing programs
This experiment is intended to add varying level astragalus polyose to laying hen immunologic function and the shadow of gut flora in research diet
Ring.Health and the close blue ash commodity egg 1440 in 380 ages in days sea of production performance are chosen in experiment, are randomly divided into 6 groups, every group 4
Individual repetition, each repeats 60 chickens.The diet based on corn-soybean meal diet, each group adds 0 in Basic drawing respectively
(I, control group), 50 (II groups), 100 (III groups), 150 (IV groups), 200 (V groups) and 250 mg/kg (VI group) astragalus polyose, in advance
1 week examination phase, 8 weeks experimental periods.Free choice feeding and drinking-water, routinely immune programme for children is immune.Basic drawing is constituted and trophic level is shown in
Table 1.Each experimental group laying hen spleen and bursa of farbricius coefficient, cecal tonsil lymph nodule quantity and villus length are observed, it is right to observe
The influence of laying hen immunologic function（It is shown in Table 2）.
The Basic drawing of table 1 is constituted
| Project | Content |
| Corn | 59.35 |
| Dregs of beans | 17.60 |
| Cottonseed Meal | 3.8 |
| The certain herbaceous plants with big flowers dregs of rice | 4.0 |
| Corn protein powder | 3.0 |
| Vegetable oil | 1.3 |
| Stone flour | 8.0 |
| Calcium monohydrogen phosphate | 1.6 |
| Salt | 0.3 |
| Methionine | 0.05 |
| Premix | 1.0 |
| It is total | 100.0 |
The collection of 1.3 samples and treatment
The 4th week vaccine inoculation H 5 N 1 avian influenza vaccine is tested, the 29th day morning took a blood sample on an empty stomach after vaccine inoculation, and every group random
4 chickens are extracted, the mL of venous blood collection 5 under wing is inclined at being statically placed in 4 DEG C after 30 min, 4 DEG C, 3 000 r/min centrifugations 10
Min obtains serum, is dispensed into -20 DEG C of preservations, test serum immune antiboidy in 1 mL centrifuge tubes.After butchering, take spleen and weigh, calculate
Spleen coefficient (the live body weight of Spleen coefficient=spleen weight/chicken).Aseptically cut open the belly and take cecal content 0.5g for micro- life
Thing culture.Bifidobacterium:BL agar mediums, 37 DEG C, the h of Anaerobic culturel 72;Bacillus acidi lactici:Modified MC agar, 37 DEG C of anaerobism
Cultivate 48 h;Escherichia coli:Mai Kangkai culture mediums, 37 DEG C of 24 h of culture.
1.4 data processings
Statistical procedures are carried out using the statistical softwares of SPSS 13.0, variance analysis uses One-way ANOVA, multiple ratio
LSD methods, data is relatively used to be represented with mean+SD, P<0.05 is significant difference, P<0.01 is that difference is extremely notable.
As a result
2.1 diets add influence of the astragalus polyose to laying hen immunologic function
The diet of table 2 adds influence of the astragalus polyose to laying hen Spleen coefficient and bird flu immune antiboidy level
| Group | Spleen coefficient | Bird flu immune antiboidy level |
| Ⅰ(0 mg/kg) | 0. 71±0. 06b | 9. 13±0. 75 |
| Ⅱ(50 mg/kg) | 0. 85±0. 08a | 9. 25±1. 06 |
| Ⅲ(100 mg/kg) | 0. 83±0. 03a | 9. 14±1. 17 |
| Ⅳ(150 mg/kg) | 0. 88±0. 03a | 9. 20±0. 92 |
| Ⅴ(200 mg/kg) | 0. 89±0. 09a | 9. 27±0. 79 |
| Ⅵ(250 mg/kg) | 0. 80±0. 07ab | 9. 58±1. 24 |
（With column data shoulder mark difference lowercase letter indication difference significantly (P<005), different capitalizations represent difference pole
Significantly (P<001), same letter or non-marking-up matrix show difference not significantly (P>0.05).Following table is same.）
From table 2, the astragalus polyose of varying level is added in laying hen diet, can to some extent improve spleen system
Number, II, III, IV, V group of experiment is all remarkably higher than control group (P<0.05).Nothing between bird flu sero-immunity antibody level each group
Significant difference (P>0.05).
2.2 diets add influence of the astragalus polyose to laying hen gut flora
The diet of table 3 adds influence of the astragalus polyose to laying hen gut flora
| Group | Escherichia coli | Lactic acid bacteria | Bifidobacterium |
| Ⅰ(0 mg/kg) | |||
| Ⅱ(50 mg/kg) | |||
| Ⅲ(100 mg/kg) | |||
| Ⅳ(150 mg/kg) | |||
| Ⅴ(200 mg/kg) | |||
| Ⅵ(250 mg/kg) |
From table 3, caecum Escherichia coli quantity, IV group of pole of experiment is substantially less than control group (P<0.01) III group, is tested
(P not notable with control group difference>0.05).Caecum lactic acid bacterium number, III group of pole of experiment is significantly higher than VI group of (P of experiment<0.01),
It is significantly higher than IV group of (P of experiment<0.05), without significant difference (P compared with control group>0.05).Caecal bifidobacteria quantity, experiment
III group of difference extremely significantly (P compared with testing VI group<0.01), without significant difference (P compared with control group>0.05) V group, is tested
With VI group of significant difference (P compared with testing I, II, III, IV group of experiment<0.05).
Conclusion
Immune organ is main by central immune organ marrow, thymus gland, bursa of Fabricius, peripheral immune organ spleen and lymphoid tissue
Deng composition.Zhang Chunhui etc. reports that the increase of blood marrow, lymph increase in mouse spleen after observing intraperitoneal injection astragalus polyose under light microscopic
It is many.Beam Warburg Pincus etc. finds that (250 mg/kg weights, once a day, continuous 5 d) not only can be notable through intraperitoneal injection for astragalus polyose
The index and spleen index (spleen weight/weight, SI) and thymus index (thymic factor D injection/weight, TI) of normal mouse, can also make burning
The SI and TI for hindering mouse recover normal.This result of the test shows, astragalus polyose can increase Spleen coefficient, with the studies above knot
Fruit is consistent.
The immune response of specific chicken body fluid after avian influenza antibody level reflection avian influenza vaccine immunity.Antibody level
Height directly reflects the humoral Immunological State of body.Astragalus polyose, fowl stream are added in this experiment in the diet for laying eggs stage laying hen
Cold and raising immunity antibody level has certain rising, but the not up to level of signifiance.
Normal flora in animal intestinal tract is important nonspecific defense barrier, for the pathogenic microorganism that some are invaded
With obvious antagonism.A kind of dynamic equilibrium is remain between normal flora, the normal physiological function with host has
Close relationship.Japanese plum roc etc. reports that astragalus polyose can increase chick enteron aisle lactic acid bacteria and bifidobacteria, reduces big
Enterobacteria quantity, moreover it is possible to make chick ileum and notable elongated, the thicker, arrangement of caecum fine hair tight, goblet cell between epithelial cell
And lymphocytosis.This result of the test shows that appropriate astragalus polyose can reduce Escherichia coli quantity in caecum, increases lactic acid bacteria
And bifidobacteria, the result of the test with forefathers is basically identical, it was demonstrated that：The astragalus polyose that varying level is added in diet can be
Spleen coefficient is improved to a certain extent；The appropriate astragalus polyose of addition can reduce caecum Escherichia coli quantity, increase lactic acid bacteria
And bifidobacteria；Composite score, adds 100 mg/kg astragalus polyose effects preferable in laying hen diet.
2nd, Inokopolyose pharmacological activity test：
Influence of the experiment Inokopolyose to immunologic function, blank control group, model group, Inokopolyose group and perfume (or spice) are divided into by chicken
Mushroom polysaccharide positive controls, ip endoxan 3d causes chicken immune to suppress model, while ig physiological saline and corresponding respectively
After the d of medicine 7 determine Inokopolyose to Immune Organs of Chichen, spleen weight, phagocytic percentage, phagocytic index, hemolysin, haemolysis spot and
The influence of lymphocyte transformation rate.
Material
1.1 animals
Breeder 150.
1.2 instruments
BI-2000 medical image analysis systems (Chengdu Tai Meng scientific ＆ technical corporation);The desk-top centrifugation of TDL-40B type low speed
Machine (Anting Scientific Instrument Factory, Shanghai);DY89-1 type electric driven glass refiner (the new limited public affairs of sesame biotechnology share in Ningbo
Department);UV-2201 ultraviolet-visible spectrophotometers (Japanese Shimadzu) etc..
1.3 medicines and reagent
Inokopolyose of the present invention;Endoxan (the permanent auspicious medical limited public affairs of share in cyclophosphamide, CTX Jiangsu
Department, lot number 08110921);Lentinan piece (Hubei Guangren Pharmaceutical Co., Ltd., lot number 080702);Plant blood
Solidifying element (Shanghai Yi Hua medical science and technologies Co., Ltd, lot number 20085601);(Sichuan Province's mikey science and technology is limited for Wright's stain
Company, lot number 0908033);Sodium citrate (factory of Tianjin chemical reagent three, lot number 20020228) etc..
The preparation of 1.4 positive control solutions
The g of lentinan film 0. 261 3 is taken, 6 gL are made with 0. 5% carboxymethylcellulose sodium solution- 1Suspension
Liquid.Storage refrigerator is standby.
Testing program
2.1 Peritoneal Macrophage Phagocytosis are determined
Chicken 40, is randomly divided into 4 groups, every group 10, respectively blank control group, model group, Inokopolyose group,
Lentinan positive controls.In addition to blank control group, the gkg of remaining each group chicken ip CTX 0. 08- 1, daily 1
It is secondary, continuous 3 d.Ig administrations simultaneously, blank control group and model group give physiological saline, Lentinan positive controls
Give the gkg of Lentinan piece suspension 0.12- 1, Inokopolyose group gives Inokopolyose aqueous solution 0.5gkg- 1, often
Its administration 1 time, continuous 7 d.7th day morning 5% red blood cell normal saline suspensions of every ip, 0. 5 mL, in giving
4h after red blood cell, 2 h put to death chicken after last ig, take result by literature method and calculate phagocytic rate and phagocytic index.
Phagocytic rate=(number of macrophages/100 macrophage of phagocytosis chicken red blood cell) × 100%
Sum/100 of the red blood cell that phagocytic index=100 macrophage is swallowed
2.2 thymus gland and spleen weight are determined
Each group chicken in method 2.1 is taken out thymus gland and spleen and weighs, and calculates thymus gland, index and spleen index:Spleen (thymus gland) refers to
Number=spleen (thymus gland) quality (mg)/body weight (g)
2.3 chicken hemolysins are formed and determined
Chicken packet, modeling and administration are with 2.1.In administration the 1st day, per the red blood cell normal saline suspensions of mouse ip 5%
Only (being immunized), 2 h after being administered in last 1 time take blood centrifugation to 0. 2 mL/, take serum.Result is taken by literature method, is surveyed
Determine each group hemolysin formational situation.
2.4 haemolysis spots are formed and determined
Each group chicken in method 2.3 is put to death, and dissects chicken, takes out spleen, and same group of chicken spleen is placed on into one two-by-two at random
Rise, spleens cell number is 5 × 106/mL in being homogenized adjustment splenocyte suspension with homogenizer.Result is taken by literature method,
Survey each group hemolysis plaque formational situation.
2.5 lymphocyte transformations are determined
Chicken packet, modeling and administration are with 2.1.In being administered preceding 3 d, daily every equal intramuscular injection phytohemagglutin phytolectin (
Phytohaemagglutinin, PHA) 0.008 gkg- 1, in 2 h after last 1 time administration, take blood, push jack, Switzerland's dye
Liquid is dyeed, and oil mirror observes the conversion ratio of PBLC.
2.6 statistical procedures
Data are represented with x ± s, and variance analysis is carried out to data with SPSS 13.0, are compared between carrying out group using t inspections.
P ＜ 0.05 are statistically significant.
Conclusion
3.1 pairs of endoxan immunosupress chicken Peritoneal Macrophage Phagocytosis and thymus gland, the influence result of spleen weight
Show that model group phagocytic rate, phagocytic index and thymus gland, index and spleen index are substantially reduced, it was demonstrated that modeling success;Compare ox with model group
Knee polysaccharide group can significantly improve phagocytic percentage, phagocytic index and the index and spleen index (P of immunosupress chicken peritoneal macrophage
＜ 0.01 or P ＜ 0.05), the thymus index of Inokopolyose group is changed significantly (P ＜ 0.05), is shown in Table 1.
The sample of table 1 is to And Utilization of CTX-Immunosuppressed-mice Peritoneal Macrophage Phagocytosis and thymus gland, spleen weight
Influence (x ± s, n=10)
| Group | Phagocytic rate/% | Phagocytic index | |||
| Normal control | - | ||||
| Model | 0.08 | ||||
| Lentinan | 0.12 | 1.02±0.27 | 3.93±1.18 | ||
| Inokopolyose | 0.5 |
Note:Compare 1) P ＜ 0.05,2 with Normal group) P ＜ 0. 01;Compare 3) P ＜ 0. 05 4 with model group
) P ＜ 0.01.
3.2 pairs of endoxan cause the influence result of immunosupress chicken hemolysin, the formation of haemolysis spot and lymphocyte transformation
Show, model group is promoting hemolysin, haemolysis spot to be formed and lymphocyte transformation rate side compared with physiological saline group and Inokopolyose group
Face difference between the effects significantly (P ＜ 0. 05 or 0. 01), illustrate modeling success, and Inokopolyose group is improving this 3
Act on notable in index, be shown in Table 2.
The sample of table 2 to And Utilization of CTX-Immunosuppressed-mice hemolysin, haemolysis spot formed and lymphocyte transformation influence (
X ± s, n=10)
| Group | Hemolysin formation/A | Haemolysis spot formation/A | Lymphocyte transformation rate/% | |
| Normal control | - | |||
| Model | 0.08 | |||
| Lentinan | 0.12 | |||
| Inokopolyose | 0.5 |
Note:Compare 1) P ＜ 0.05,2 with Normal group) P ＜ 0. 01;Compare 3) P ＜ 0. 05 4 with model group
) P ＜ 0.01
Test result indicate that, Inokopolyose has stronger activity in raising immunology.The ox on immunologic function is improved
Knee polysaccharide can equally play a part of to improve immunosupress chicken immuological function.
3rd, ram's horn grass polysaccharide pharmacological activity test：
This project makes immunosuppression model using endoxan molding method, and gastric infusion, observes and to compare radix ranunculi ternati more
Sugar is to its Peritoneal Macrophage Phagocytosis, hemolysin formation and the influence of periphery blood T lymphocyte number.This experiment is mainly carried
Ram's horn grass polysaccharide is taken to determine whether with immunocompetence.
Equipment
1.1 materials
Ram's horn grass polysaccharide of the present invention.
1.2 reagents
Endoxan (SHANXI POWERDONE PHARMACEUTICAL.,LTD, lot number 20051201, mg × 5 bottle of specification 200);Physiological saline
(Zhengzhou chemistry pharmaceutcal corporation, Ltd, lot number 010409, ml/ bottles of specification 500);Rui Shi pigments (factory of Shanghai reagent three, lot number
99030);It is pure that remaining reagent chemicals is analysis.
1.3 instruments
BS210S electronic balances (Beijing Sai Duolisi balances Co., Ltd, the mg of max210 g, d 0. 1);KQ-500DV
Type numerical control ultrasonic cleaner (Kunshan Ultrasonic Instruments Co., Ltd.);The desk-top Large Copacity centrifuge of TDL-40 Type B low speed (on
Hai'an booth scientific instrument factory);SHZ-D (III) circulating water types vavuum pump (Ying Yu Yu Hua instrument plants of Gongyi City);Digital biology microscope
Camera chain (Shanghai Bi Aimu optical instruments Manufacturing Co., Ltd);(upper Nereid is grand for DHG-9076A electric heating constant-temperature blowing drying boxes
Experimental facilities Co., Ltd);Slide (Yancheng City Li Fan Glass Co., Ltd.).
1.4 animals
Breeder;Red blood cell and GPS (Medical School of Zhengzhou University animal experimental center).
1.5 ram's horn grass polysaccharide test solutions are prepared
1.5.1 Hank ' s applications liquid with producing
The 9 parts of mixing packing of 1 part of Hank ' s stostes plus distilled water, the min autoclavings of 69. 75kPa (10P/cm2) 20,
4 DEG C of refrigerators are standby.
1.5.2 Incubating Solution (esterase dyeing liquid) with producing
The ml of phosphate buffer 89 of 1/15mol/L pH 7. 6 is slowly added into after six azo paramagenta 6ml fully mix,
The ml of 2% α, 2 naphthylacetate solution 2.5 is slowly added dropwise again fully to mix, finally in amber muddy sample liquid, pH to 6.4 is adjusted.
Testing program
The influence of 2.1 radix ranunculi ternati Polysaccharides in Chicken Peritoneal Macrophage Phagocytosis
Chicken is taken to be randomly divided into by body weight:1. blank group, 2. CTX model groups, 3. treatment group, the 4. mgkg of polysaccharide 100-1.Press
0. 2 ml·kg-1Gastric infusion, 1 time/d, continuous 7d, blank group is to normal saline.In addition to blank group, polysaccharide group,
ip CTX 40 mg·kg-1Prepare immunodeficiency models.The 7th day red blood cell normal saline suspension of every intraperitoneal injection 5%
(0.2 ml/10g), after red blood cell 6h and last 1 gavage 2h is injected, puts to death chicken, with cotton ball soaked in alcohol sterilization skin of abdomen, abdomen
Hank ' s liquid 2.5ml are injected in chamber, gently rub chicken belly, and an aperture is cut on peritonaeum, and drop is a little on slide after suctioning out peritoneal fluid
And push jack, slightly dry rear Wright's stain dyeing, running water rinses, dries, basis of microscopic observation.Chicken peritoneal macrophage is calculated to chicken
Red blood cell phagocytic percentage and phagocytic index, wherein phagocytic percentage=(phagocyte number/100 macrophage of phagocytosis chicken red blood cell
Cell) × 100%;Chicken red blood cell number/100 that phagocytic index=100 macrophage is swallowed.The results are shown in Table 1.
The ram's horn grass polysaccharide of table 1 aligns the influence of normal and immunosupress chicken Peritoneal Macrophage Phagocytosis
| Group | Phagocytic percentage (%) | Phagocytic index | |
| Blank | - | 64.6±11.8 | 0.69±0.11 |
| Model | 40 | 32.6±8.35* | 0.36±0.09* |
| Treatment | 40 (ring)+600 (polysaccharide) | 59.5±6.24△ | 0.68±0.05△ |
| Polysaccharide | 600 | 70.8±7.09* | 0.79±0.11* |
Compare with control group, * P<0. 05;Compare with model group, △ P<0. 05;n=10
The above results illustrate that ram's horn grass polysaccharide can significantly improve normal chicken peritoneal macrophage phagocytic percentage and phagocytosis refers to
Number, and can antagonism caused by cyclophosphamide immunosuppressive action.
2.2 ram's horn grass polysaccharides align the influence that normal and immunosupress chicken hemolysin is formed
Take chicken to be grouped as stated above, each group is in chicken red blood cell, the physiological saline suspension per mouse intraperitoneal injection 5% in the 1st day
The ml of liquid 0. 2 is immunized, 2 h after being administered in the 7th day, and corn socket of the eye takes blood, and centrifuging and taking serum is diluted with physiological saline 1: 100
Afterwards, the ml of 0.5 ml of chicken red blood cell suspension, 10% complements 0.5 for taking 1ml and 5% is mixed, and separately sets the control tube of not increase serum, will
Suspension is fully mixed, and 30min is incubated in 37 DEG C of insulating boxs, the stopped reaction in frozen water, centrifugation, takes supernatant in 540 nm
Place's colorimetric, " 0 " point is adjusted with blank tube, surveys the trap of each pipe.The results are shown in Table 2.
The ram's horn grass polysaccharide of table 2 aligns the influence that normal and immunosupress chicken hemolysin is formed
| Group | Hemolysin (A) | |
| Blank | - | 0.306±0.125 |
| Model | 40 | 0.038±0.289* |
| Treatment | 40 (ring)+600 (polysaccharide) | 0.210±0.114△ |
| Polysaccharide | 600 | 0.509±0.254* |
Compare with control group, * P<0. 05;Compare with model group, △ P<0. 05;n=10
Result shows that ram's horn grass polysaccharide can improve the content of normal chicken serum hemolysin, and energy antagonism endoxan to body
The immune inhibitory action of liquid.
2.3 ram's horn grass polysaccharides align the influence of normal immunosupress chicken periphery blood T lymphocyte number
Take chicken to be grouped as stated above, in the 1st day every mouse intraperitoneal injection of cyclophosphamide (40 mg/kg, 0.1 of gavage
Ml/10g), continuous 8d, separately sets a complete blank control group, and intraperitoneal injection simultaneously gavages the physiological saline of same volume.In last dose
1 h, plucks eyeball and takes blood push jack afterwards, after natural-wind dry, 37 DEG C of 3 h of incubation, running water in immersion Incubating Solution (30 min of preheating)
(to rinse sediment as degree) is rinsed a moment, solid carbon dioxide pearl is received with filter paper, plus 1% malachite green solution dyes 5~10s, running water is rinsed,
Filter paper is gently inhaled, and dries rear oil mirror inspection.Lymphocyte nuclear is larger and rounded not leaflet, dyes green, and endochylema is in hyacinthine.T
The dark red coloured particles (general 2~5) that size and number is not waited are presented in lymphocyte endochylema, in the endochylema of non-T lymphocytes
Without particle.100 lymphocytes are counted, the percentage of T lymphocytes is calculated, compares administration group and control group chicken peripheral blood T
The difference of cent lymphocytes.The results are shown in Table 3.
The ram's horn grass polysaccharide of table 3 aligns the influence of normal and immunosupress chicken periphery blood T lymphocyte number
| Group | Peripheral blood T cells percentage (%) | |
| Blank | - | 57.70±5.83 |
| Model | 40 | 35.56±5.13* |
| Treatment | 40 (ring)+600 (polysaccharide) | 55.75±7.99△ |
| Polysaccharide | 600 | 63.80±3.26* |
Compare with control group, * P<0. 05;Compare with model group, △ P<0. 05;n=10
As can be seen from the above experimental data, endoxan group T cell percentage is significantly lower than blank group, and treatment group
T cell compares equal no significant difference with blank group, the suppression that this explanation ram's horn grass polysaccharide can be with antagonism endoxan to cellular immunity
Make and use.And polysaccharide also has obvious humidification to the cellular immunity of normal chicken.
Conclusion
Through another circulating antibody-hemolysin (IgM) in the animal blood serum that chicken red blood cell sensitization is crossed, with chicken through together with cell
It is external to incubate, produce hemolytic reaction in complement presence.The content of hemolysin can be by haemolysis process in sensitized animal serum
The hemoglobin content of middle release is detected.Hemolysin level reflects body fluid immunocompetence, is in positive with humoral immunity of organism ability
Relation.Ram's horn grass polysaccharide can improve the content of chicken serum hemolysin, and the humoral immunity of energy antagonism caused by cyclophosphamide suppresses, and says
Bright ram's horn grass polysaccharide can improve humoral immunity of organism ability, and clinic is acted on antitumor and treating tuberculosis, with the immune work of excitement
Property, it is that clinical practice and exploitation provide technical foundation.
4th, adjuvant effect of the semen momordicae frost to avian influenza antigen：
60 two week old chick are chosen, 1) H 5 N 1 avian influenza vaccine control group is grouped according to following；2) H 5 N 1 avian influenza epidemic disease
The μ g of seedling+20 semen momordicaes frost group；3) the μ g semen momordicaes of H 5 N 1 avian influenza vaccine+40 frost group；4) the μ g Momordica cochinchiensis of H 5 N 1 avian influenza vaccine+80
Sub- frost group.Taken a blood sample respectively within the 7th, 14 and 28 days before being immunized and after being immunized, determine total antibody OD values.
Result shows that total antibody mean OD value of all semen momordicae frost groups is all higher than control group, but only 28th after immune
It semen momordicae frost group has significant difference (P compared with the control<0.05).Meanwhile, the chicken of 20 μ g semen momordicaes frost group averagely increases day by day
Control group (P is significantly higher than again<0.05).Showing the use of semen momordicae frost will not produce harmful effect to daily gain.
5th, clinical test of the compound Radix Astragali polysaccharide particle of the present invention to bird flu
1 materials and methods
1.1 test materials
Compound Radix Astragali polysaccharide particle of the present invention；Amantadine is purchased from Beijing North medicine medication chemistry company.
1.2 test chickens are selected and packet
Test arrangement is in 3 closed hen houses in different onset stage in certain chicken house, adopts pathological material of disease, blood sample through AGP, HI examinations
Test, Bacteria Culture, virus purification combination clinical symptoms, pathology cut open inspection are diagnosed as bird flu (AI) H9 hypotypes.First 3036, it is complete
It is randomly divided into test group 1520, control group 1516 entirely;3057 completely randoms of second are divided into test group 1530, control group
1527;Third 3086, completely random is divided into test group 1540, control group 1546.3 are 28 week old sieve for examination chicken
Graceful brown commercial generation laying hen.First chicken group is the 3rd age in days of morbidity, and symptom (in incubation period), third not yet occurs in second chicken group
Chicken group is the 14th age in days after morbidity.
1.3 feedings and managements
Feeding and management is carried out by normal feeding and management method per house, each feeding and management condition is identical, each test group
Its corresponding control group management method is also just the same.
1.4 testing programs
Each control group is drunk water with the amantadine of 160 mg/L, and test group is drunk water with compound Radix Astragali polysaccharide, tests first agent
It is 200 mg/L to measure, and experiment second, third group of dosage are 160 mg/L, continuous use 14d.The essence of each group chicken is observed and recorded daily
Situations such as god, feeding, drinking-water, excrement, egg number, morbidity number and death toll.In this experiment, second chicken group is the 4th of experiment
It two groups start morbidity, 6th day i.e. initial latter 20th day recurrence bird flu H9 hypotype of third control group of chicken group in experiment.
2 results and analysis
Result of the test is shown in Table 1.As can be seen from the test results, each test group and control group laying rate level during on-test
Quite (P>0.05), in-test, each test group is substantially better than corresponding control group in all indexs, each test group with it is corresponding
Control group compare, first performance is identical on the incidence of disease, second performance significant difference (P<0.05), third performance is extremely notable
Sex differernce (P<0.01);Not notable (the P of two performance differences of first, second in the death rate>0.05), third performance difference extremely significantly (P
<0.01);First, second, the third 3 performance difference extremely significantly (P in laying rate fall<0.01);At lopsided (soft-shelled egg etc.)
First, second in egg rate, the third 3 performance difference significantly (P<0.05).
The result of the test of table 1
3 conclusions
This disease is combined in local epidemic characteristic by the result of this controlling experiment, it can be deduced that these points conclusion:
(1) 3 stage application compound Radix Astragali polysaccharide particles of the present invention add preventing and treating before, during and after bird flu morbidity,
The amplitude that the death rate and laying rate of chicken decline can be substantially reduced, compound Radix Astragali polysaccharide has obvious antiviral, anti-inflammatory effect；
It is substantially better than amantadine；
(2) the resistance to group of chicken excessively of first attack obviously can obtain certain immunity to this disease, but because this disease destroys chicken immune
System, many chicken groups often have after initial 20~30d recurs this disease to some extent, if addition compound astragalus membranaceus after initial
Polysaccharide can effectively prevent this palindromia；
(3) it is substantially better than in terms of laying rate decline, eggshell quality recovery is reduced using the anti-disease of effecting a permanent cure of compound Radix Astragali polysaccharide
Amantadine；
(4) traditional Chinese medical science thinks that the Radix Astragali has the drug effect of " tonifying middle-Jiao and Qi, Gu table rises sun, myogenic, consolidates in holding up ".Compound astragalus membranaceus are more
Sugar is formed by exquisite refining by Chinese herbal medicine astragalus, and main component is the panimmunity reinforcers such as astragalus polyose I, astragalus polyose II
Matter, then Inokopolyose, ram's horn grass polysaccharide and semen momordicae frost are equipped with, support mutually, can a large amount of leucocyte interference of excitating organism generation
Element and phagocyte, lymphocyte；And internal humoral immunity approach can be strengthened, produce substantial amounts of immunoglobulin and benefit
Body, improves microcirculation, effectively nurses one's health and strengthen the humoral immunity and cellular immunity of body, body's immunity is improved, to disease
Poison produces interference, phagocytosis, combination, and number of ways kills virus and bacterium, and has clearing heat and detoxicating and regulation organism metabolism machine
Can be disorderly the effect of, strengthen birds resistance against diseases.
The present invention obvious curative effects are obtained in clinical practice, in order to be preferably applied for clinical applicant according to《New drug is noted
Volume management method》Middle registration declaration material requirement, Mouse Acute Toxicity experiment and rat long term toxicity test are carried out to said preparation.
The Mouse Acute Toxicity of result of the test explanation compound Radix Astragali polysaccharide granule is smaller, points out larger dose application in clinical a day to be somebody's turn to do
Preparation will not produce toxic reaction.Toxicity to the long-term oral administration of rat is relatively low, and security is preferable.
6th, effect of compound Radix Astragali polysaccharide particle of the present invention
Effect of compound Radix Astragali polysaccharide of the present invention：With stronger clearing heat and detoxicating, strengthening vital QI to eliminate pathogenic factors, tonifying spleen kidney is trained, QI invigorating is supported
Blood, broad-spectrum antiviral, antibacterial can induce body to produce interferon, promote antibody to be formed, and the immunity and body for strengthening body are each
The functions such as system repair ability, disease-resistant growth-promoting, resisting stress.Especially to the avian influenza prevention best results of birds.
1）The application of broad-spectrum antiviral：Compound Radix Astragali polysaccharide particle is a kind of antiviral and interferon inducer of wide spectrum,
It can stimulating expression of macrophage and T cell function, E rings is formed cell number increases, and Cytokines Production promotes interleukins
Induce.So that animal body produces endogenous interferon, so as to reach antiviral purpose.For treating bird flu, infecting
The viral infectious such as property bursal disease.The Radix Astragali can the preventing cold reduction incidence of disease more than 80%, astragalus polyose and Inokopolyose,
Ram's horn grass polysaccharide, semen momordicae frost use in conjunction can reduce the incidence of disease more than 90%；
2）Strengthen the application of vaccine immunity power：Compound Radix Astragali polysaccharide particle is gone back in addition to itself having enhancing body name epidemic disease power
The immunity of vaccine seedling is greatly enhanced, and there is good protecting effect to vaccine seedling, according to research reports astragalus polyose energy
The serum for strengthening Mareks disease vaccine draws body potency.Compound Radix Astragali polysaccharide particle is used in combination to improve with NDV, IV vaccine exempts from
The Serum HI antibody potency of chicken after epidemic disease, coordinates compound Radix Astragali polysaccharide particle when using coccidial vaccine, can strengthen Eimeria species and be immunized
Protection；
3）Effect to liver：Compound Radix Astragali polysaccharide particle can promote liver cell DNA, RNA to synthesize, and mitigate Hepatoxic substance
The lesion for causing, strengthens liver detoxification function, has protective effect to liver cell.To chicken fatty liver, hydropericardium hepatitis syndrome,
Big hep, hepatitis splenomegaly syndrome have good prevention effect, while livestock and poultry feed intake can be improved, so as to promote livestock and poultry
Grow；
4）To renal effect：Compound Radix Astragali polysaccharide particle can improve nephrocyte metabolism and renal function, reduce serum creatinine level,
Renal failure after the nephrectomy can be alleviated.To nephrotic syndrome, acute renal failure, nephrotic syndrome, kidney enlargement lithate
The diseases such as deposition have good prevention effect.Strong kidney purpose can be played simultaneously, is conducive to growth of animals or poultry to breed the performance of function；
5）Improve reproductive performance：Compound Radix Astragali polysaccharide particle has stablizes cell, anti-aging, Green Tea Extract and peroxidating
Effect, periodically uses for a long time, can significantly improve the service life and service efficiency of breeder.Additionally, compound Radix Astragali polysaccharide particle has
There is rush sexual gland to act on, the weight of endometrial thickness, uterine cavity expansion, cornua uteri and ovary can be made to jenny to be increased.
Compound Radix Astragali polysaccharide particle can also improve the reproductive performance of buck, can make spermatozoon activity, living spermatozoa percentage, sperm concentration
Etc. being significantly increased；
6）It is the 4th needs of chick health care：The health care of current poult is made an issue of at three aspects mostly：(1) prevent virus diseases
Vaccine；(2) the antibiotic of bacterial disease is prevented；(3) build up health, the nutriment of enhance immunity.By long-term practical studies
It was found that, while chick three of the above health care needs are met, substantial chick health care also there are " the 4th needs ", that is,
Compound Radix Astragali polysaccharide particle.It can not only give full play to three of the above health-care effect, moreover it is possible to meet chick health care residual without medicine
Stay, the need for giving full play to its growth potential；
7）With nutrient utilization is improved, promote the function of growth of animal.The acid of compound Radix Astragali polysaccharide particle embedded amine groups,
Various nutriments such as vitamin, trace element, and UGF（UGF）.It is remarkably improved as feed addictive
The speed of growth of livestock and poultry, lifts Abwehrkraft des Koepers, improves egg quality and yield.
In a word, compound Radix Astragali polysaccharide particle of the present invention has：
1）For prevent and treat birds low pathogenicity bird flu clinical prevention and treatment and highly pathogenic bird flu it is effective
Immune prevention and control；
2）Effectively treat and prevent poultry body immunologic hypofunction（The organs such as thymus gland, liver, kidney, the bursa of farbricius wither
Contracting）；
3）, feed intake slow to the poultry speed of growth is low, regularity is poor, and egg fowl is without laying eggs peak or unknown cause subtracts egg
And intractable watery diarrhea, prolapse of the anus etc. have special efficacy；
4）This product has protection to vaccine and acts synergistically, hence it is evident that lifting immunizing potency, can simultaneously be used with vaccine；
5）This product is used in conjunction with coccidiostat and can significantly improve anticoccidial index；
6）The preventing and treating various viral diseases of livestock and poultry, such as PCV-II, blue otopathy, influenza, pseudo- mad dog, viral abdomen
Rush down, repeated relapsing asthma and nameless high-fever etc.；
7）Clinical application effect is good, and granule market is rare, more competitive than popular pulvis, high in technological content,
Added value is big；
8）Ultra micro spiral particle preparation process, advantage is distinct, is easy to preserve and clinical practice, is more easy to cut market；
9）Proterties stabilization, water-soluble fabulous, nontoxic, harmless, no drug residue；
10）Spice is convenient uniform, and product development has a extensive future.
Compound Radix Astragali polysaccharide particle of the present invention by studying for many years, clinical test and to practice summary, it is determined that our prescription
It is feasible, effectively, it is worthy to be popularized, effective for the preventing and treating of the virosis such as bird flu, research success of the invention will be with exploitation
Social creativity goes out great economic benefit and far-reaching social benefit.
Claims (4)
1. it is a kind of prevent and treat bird flu compound Radix Astragali polysaccharide particle, it is characterised in that be by following weight meter：Astragalus polyose
550-650g, Inokopolyose 180-220g, ram's horn grass polysaccharide 110-130g and semen momordicae frost 75-85g make material medicine, then add
The auxiliary material of material medicine weight 3% is prepared into particle, and described astragalus polyose is the dry Radix Astragali, is added water, and extracts three times, first
Secondary extraction, first soaks 30-60min with the water of 12 times of weight of the Radix Astragali, is fried boiling, then is cooked by slow fire 1.5 hours, filters, and obtains the first filtrate,
The dregs of a decoction add 10 times of water of weight of the Radix Astragali, carry out second extraction, boil, then are cooked by slow fire 1.5 hours, filter, and obtain the second filtrate,
The dregs of a decoction add 10 times of water of weight of the Radix Astragali again, carry out the third leaching, boil, then are cooked by slow fire 1 hour, filtering, obtain the 3rd filtrate, close
And three filtrates, it is concentrated into 1：1 concentrate, is precipitated with the absolute ethyl alcohol of 4 times of volumes of concentrate, and drying precipitate obtains yellow
Astragalus polysaccharides；Described Inokopolyose is, by the dry root of bidentate achyranthes, to add water, solid-liquid ratio 1:15,100 DEG C of extraction 2h, leaching liquor adds the root of bidentate achyranthes
5 times of ethanol of the mass concentration of weight 95%, are extracted 2 times, and 2 hours every time, merging filtrate, concentration was dried, and obtains Inokopolyose；Institute
The ram's horn grass polysaccharide stated is that radix ranunculi ternati adds water, and is extracted 2 times, and each amount of water is 12 times of radix ranunculi ternati weight, and 3 are extracted every time
Hour, merge leaching liquor twice, be concentrated into equivalent to the concentrate containing crude drug 800g/L, plus the volume of concentrate 50% anhydrous second
Alcohol, puts 6 hours only, discards upper liquid, obtains sediment, as drying precipitate, ram's horn grass polysaccharide；Described semen momordicae frost is
By Semen Momordicae, load the compacting of squash type making drugs into frostlike powder machine, control temperature for 100 DEG C, when oil pump capacity reaches 17-20%, stop squeezing,
The dregs of a decoction are pulverized and mixed uniformly, semen momordicae frost is obtained；Described auxiliary material is one or more mixed of starch, Icing Sugar, dextrin
Close.
2. it is according to claim 1 preventing and treating bird flu compound Radix Astragali polysaccharide particle, it is characterised in that be by following weight
Meter：Astragalus polyose 550g, Inokopolyose 180g, ram's horn grass polysaccharide 110g and semen momordicae frost 75g make material medicine, add raw material
The auxiliary material of drug weight 3% is prepared into particle, and described auxiliary material is starch, Icing Sugar, dextrin, by weight 1：3：1 mixture it is uniform and
Into.
3. it is according to claim 1 preventing and treating bird flu compound Radix Astragali polysaccharide particle, it is characterised in that be by following weight
Meter：Astragalus polyose 600g, Inokopolyose 200g, ram's horn grass polysaccharide 120g and semen momordicae frost 80g make material medicine, add raw material
The auxiliary material of drug weight 3% is prepared into particle, and described auxiliary material is starch, Icing Sugar, by weight starch：Icing Sugar=1：3 mix and
Into.
4. it is according to claim 1 preventing and treating bird flu compound Radix Astragali polysaccharide particle, it is characterised in that be by following weight
Meter：Astragalus polyose 650g, Inokopolyose 220g, ram's horn grass polysaccharide 130g and semen momordicae frost 85g make material medicine, add raw material
The auxiliary material of drug weight 3% is prepared into particle, and described auxiliary material is starch, dextrin, by weight 1：1 mixture is uniformly formed.
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| CN104998034A (en) * | 2015-08-07 | 2015-10-28 | 河南省睿兰特生物制药有限公司 | Chinese herbal medicine combination drug treating livestock respiratory diseases and preparation method thereof |
| CN105535012B (en) * | 2016-01-27 | 2018-03-13 | 中国人民解放军疾病预防控制所 | A kind of broad spectrum activity inhibitors of viral infection |
| CN105560351B (en) * | 2016-01-27 | 2019-10-11 | 中国人民解放军疾病预防控制所 | It is a kind of for inhibiting the preparation of adenovirus infection |
| CN105560268B (en) * | 2016-01-27 | 2018-03-23 | 中国人民解放军疾病预防控制所 | A kind of preparation for being used to suppress influenza infection |
| CN105663218B (en) * | 2016-01-27 | 2019-10-11 | 中国人民解放军疾病预防控制所 | It is a kind of for inhibiting the preparation of poxvirus infection |
| CN107661489A (en) * | 2017-11-14 | 2018-02-06 | 商丘美兰生物工程有限公司 | Vaccine Chinese traditional immunopoteniators and preparation method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101385772A (en) * | 2008-01-09 | 2009-03-18 | 姜宝库 | Effervescence tablets for increasing natural immunity |
-
2014
- 2014-07-30 CN CN201410368695.9A patent/CN104083427B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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Non-Patent Citations (4)
| Title |
|---|
| 复方黄芪多糖防治禽流感临床试验;张靖飞，等。;《动物医学进展》;20030520;第24卷(第3期);第125页最后一段。 * |
| 木鳖子提取物对于口蹄疫、禽流感及法氏囊疫苗的免疫佐剂作用研究;Zahid Iq bal Rajput;《中国博士学位论文全文数据库》;20110515;摘要 * |
| 牛膝多糖对雏鸡禽流感免疫效果的影响;刘永华，等。;《饲料研究》;20140507;第57页最后一段 * |
| 猫爪草多糖免疫调节及抗氧化活性研究;吕小华，等。;《中国中药杂志》;20100715;第35卷(第14期);第1864页右栏第一段。 * |
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