CN104026154B - A kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide and application thereof - Google Patents
A kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide and application thereof Download PDFInfo
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- CN104026154B CN104026154B CN201410300518.7A CN201410300518A CN104026154B CN 104026154 B CN104026154 B CN 104026154B CN 201410300518 A CN201410300518 A CN 201410300518A CN 104026154 B CN104026154 B CN 104026154B
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide and preparation method thereof, it is as follows that formula presses mass percent: Parasitism broom bacterium conidial powder 5 ~ 30%, wetting dispersing agent 8 ~ 20%, uv-protector 0.1 ~ 1%, disintegrant 2 ~ 20%, binding agent 1.0 ~ 10% and carrier 19 ~ 83.9%.Parasitism broom bacterium conidium dried bean noodles suspension bactericide is as the application of disease of the control pathogenic ring rot of apple of agricultural and sclerotinia rot of colza.The present invention is Parasitism broom bacterium dry suspending agent, improves conidial germination rate and suspensibility, wetting and disintegration rate fast, dispersed and heat storage stability is excellent.Have conidium survival rate high (80.16%), long shelf-life, disintegration soon, suspensibility is high, particle diameter is thin, the advantage such as strong adhesion, resistance to erosion, is a kind of excellent environment friendly agricultural.
Description
Technical field
The present invention relates to a kind of microbial pesticide, specifically its application of a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide.
Background technology
Parasitism broom bacterium (Clonostachysrosea), is subordinate to hyphomycete, is the Hyperparasite that a class is extensively present in the plant pathogenic fungi in soil.The research such as T.Yh-Mattila finds that it is widely distributed in the soil containing fertilizer.Can the mycelia of parasitic various plants disease fungus and sclerotium, also there is the effect of Promoting plant growth simultaneously.
Parasitism broom bacterium can belong to the various plants pathogens such as (Sclerotinia), pythium spp (Pythiumdebaryanum), Rhizoctonia solani Kuhn (Rhizoctoniasolani) and Sclerotium rolfsii (Sclerotiumrolfsii) by Sclerotinia sclerotiorum.Sticky broom bacterium has phytopathogen and produces the multiple action such as antibiotic and toxin, hyperparasitism, generation cell wall degrading enzyme and synergy mechanism, can effectively suppress fungal diseases of plants generation.The report of broom bacterium granule is glued in external existing commercialization, and different strains is made biologic product, for preventing and treating the stalk breaks such as pea, soybean, sunflower, rape, lettuce and other multiple greenhouse and field crop soil-borne disease.Dry suspending agent is the pesticides new formulation and novel formulation process technology that start in the world the eighties in 20th century to research and develop.This formulation has the graininess formulation of the solid-liquid dispersing system being dispersed into higher suspension into the rapid disintegration of water, and not containing organic solvent, suspension stability is good, suspensibility is high, without dust, packaging, transport, easy to use.Dry suspending agent is considered to the advantage both with granula, has again desirable formulations of pesticide of suspending agent high dispersive performance, has become the focus of novel form exploitation.Because the biocompatibility of Parasitism broom bacteria strain to auxiliary agent is poor, so far there are no the market sale of its commercial preparation and field are widely applied.
Summary of the invention:
Lacking for gluing broom microbial inoculum type at present, the invention provides its application of a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide.The present invention utilize its conidial powder make water slurry, water slurry through moment mist projection granulating be prepared into dry suspending agent.
The technology used in the present invention is as follows: a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide, and it is as follows that formula presses mass percent: Parasitism broom bacterium conidial powder 5 ~ 30%, wetting dispersing agent 8 ~ 20%, uv-protector 0.1 ~ 1%, disintegrant 2 ~ 20%, binding agent 1.0 ~ 10% and carrier 19 ~ 83.9%.
The present invention also has following technical characteristic:
1. carrier described in is one in attapulgite, kaolin, diatomite, bentonite, white carbon or its composition.
2. wetting dispersing agent selected by is one in MorwetEFW, k12, MF-5, TERWET1010, WLNO, calcium lignosulfonate, TERSPERSE2700, TERSPERSE2020, MorwetD425, NNO, T36 or its composition.
3. disintegrant selected by is one in crospolyvinylpyrrolidone, Ac-Di-Sol, PVPP, urea, ammonium sulfate, starch or its composition.
4. binding agent selected by is one in CMC, sodium lignin sulfonate, PEG, oxalic acid, dextrin or its composition.
5. the uv-protector selected by is dextrin, xanthans, fluorescein sodium, vitamin C, the one of nano zine oxide or its composition.
6. Parasitism broom bacterium conidial powder spore content>=1 × 10 described in
10individual/g.
7. to press mass percent proportioning as follows for optimization formula: the mixture 12% of Parasitism broom bacterium conidial powder 5%, MorwetEFW and calcium lignosulfonate, crospolyvinylpyrrolidone 3%, nano zine oxide 0.2%, PEG4% and white carbon 75.8%, wherein, MorwetEFW is 1: 5 with the mixture quality ratio of calcium lignosulfonate.
8. to press mass percent proportioning as follows for optimization formula: Parasitism broom bacterium conidial powder 20%, the mixture 15% of TERWET1010 and TERSPERSE2700, PVPP 4%, vitamin C 0.3%, sodium lignin sulfonate 5% and diatomite 55.7%, wherein the mixture quality ratio of TERWET1010 and TERSPERSE2700 is 1: 4.
9, described a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide is as the application of disease of the control pathogenic ring rot of apple of agricultural and sclerotinia rot of colza.
The present invention is Parasitism broom bacterium dry suspending agent, improves conidial germination rate and suspensibility, wetting and disintegration rate fast, dispersed and heat storage stability is excellent, for control of plant disease provides new material.Have conidium survival rate high (80.16%), long shelf-life, disintegration soon, suspensibility is high, particle diameter is thin, the advantage such as strong adhesion, resistance to erosion, is a kind of excellent environment friendly agricultural.
Accompanying drawing explanation
Fig. 1 Parasitism broom bacterium conidium is schemed;
Fig. 2: the grain size distribution of Parasitism broom bacterium dry suspending agent.
Embodiment
In this test, Parasitism broom bacterium (Clonostachysrosea) is Parasitism broom bacterium by morphology and ITS-rDNA Sequence Identification, accession number: KJ941018.Derive from the State Administration of Forestry 948 project, problem title: gray mold antagonistic microbe production technology is introduced.
Embodiment 1
Parasitism broom bacterium conidial powder 5%, MorwetEFW and lignin sulfonic acid calcium compound 12%, crospolyvinylpyrrolidone 3%, PEG4%, nano zine oxide 0.2%, white carbon 75.8%, wherein, MorwetEFW and calcium lignosulfonate mixture quality ratio are 1: 5;
Embodiment 2
Parasitism broom bacterium conidial powder 20%, TERWET1010 and TERSPERSE2700 mixture 15%, PVPP 4%, sodium lignin sulfonate 5%, vitamin C 0.3%, diatomite 55.7%, wherein, TERWET1010 and TERSPERSE2700 mixture quality ratio is 1: 4;
Embodiment 3
Parasitism broom bacterium conidial powder 25%, TERWET1010, TERSPERSE2020 and T36 mixture 18%, ammonium sulfate 5%, CMC6%, nano zine oxide 0.4%, bentonite 45.6%, wherein, TERWET1010, TERSPERSE2020 and T36 mixture quality ratio is 1: 3: 2;
Embodiment 4
Parasitism broom bacterium conidial powder 7%, MorwetEFW and lignin sulfonic acid calcium compound 10%, urea 2%, oxalic acid 1%, xanthans 0.3%, kaolin and white carbon mixture 79.7%, wherein, MorwetEFW and calcium lignosulfonate mixture quality ratio are 1: 4, and kaolin and white carbon mixture quality ratio are 1: 4;
Embodiment 5
Parasitism broom bacterium conidial powder 28%, TERWET1010, TERSPERSE2020 and T36 mixture 16%, crospolyvinylpyrrolidone and PVPP mixture 20%, sodium lignin sulfonate and PEG mixture 10%, fluorescein sodium 0.6%, diatomite and white carbon mixture 25.4%, wherein, TERWET1010, TERSPERSE2020, T36 mixture quality ratio is 1: 1: 2, crospolyvinylpyrrolidone and PVPP mixture quality ratio are 1: 3, sodium lignin sulfonate and PEG mixture quality ratio are 1: 4, diatomite and white carbon mixture quality ratio are 1: 1,
Embodiment 6
Parasitism broom bacterium conidial powder 30%, TERWET1010, TERSPERSE2020 and T36 mixture 20%, crospolyvinylpyrrolidone and cross-linked methyl sodium cellulosate mixture 18%, dextrin and oxalic acid mixture 9%, nano zine oxide and xanthan gum mixtures 1%, bentonite and white carbon mixture 22%, wherein, TERWET1010, TERSPERSE2020 and T36 mixture quality ratio is 2: 3: 5, crospolyvinylpyrrolidone and cross-linked methyl sodium cellulosate mixture quality ratio are 1: 2, dextrin and oxalic acid mixture quality ratio are 2: 1, nano zine oxide and xanthan gum mixtures mass ratio are 2: 3, bentonite and white carbon mixture quality ratio are 1: 3,
Embodiment 7
By said components and proportioning after Universalpulverizer mechanical mixture evenly (except conidial powder), add appropriate water, micronization is carried out through homogenizer or sand mill, add conidial powder, shearing through high-speed shearing machine makes conidial powder be dispersed in the aqueous solution of said components, then carries out mist projection granulating.
Step one: according to weight percent meter, each auxiliary agent and carrier through Universalpulverizer premix, after adding appropriate water, then through Canadian ATS high pressure homogenizer (model AH100B) or sand mill micronization, operating pressure 500 ~ 1200bar, size controlling is at 25 μm;
Step 2: step one is obtained sample proportionally mix with Parasitism broom bacterium conidial powder after in high-speed shearing machine, 8000 ~ 15000r/min, 20 ~ 60min, carries out mist projection granulating after mixing immediately, namely obtains Parasitism broom bacterium conidial powder dry suspending agent.
Embodiment 8
Invention also provides conidium powder, preparation method thereof, conidium, to producing spore, is mixed with 3 × 10 by activation culture under the bacterial classification 25 DEG C of preservation and 12L:12D condition
8the suspension of individual spore/ml concentration, after being inoculated in blake bottle shaken cultivation 48h (25 ± 1 DEG C, 180r/min), transferring is inoculation liquid into large-scale blake bottle scale-up medium 24h.Rice (g): water (ml) soaks 20min by after 1: 0.5 mixing, cleans that to drain rear moist heat sterilization ripe to the grain of rice sixty percent, admixes inoculation after cooling by 15% inoculation liquid, cultivates 5 ~ 7d under the condition of 25 ± 1 DEG C and 12L:12D.Rice culture is collected after vacuum freezedrying process Parasitism broom bacterium conidial powder, make its moisture content be down to less than 4%, utilize vibratory sieve that spore is separated with rice matrix, survey its spore content, spore rate alive, put 4 DEG C of conditions for subsequent use.
The screening of described auxiliary agent is carried out mainly for biocompatibility, each auxiliary agent (wetting dispersing agent, uv-protector, disintegrant, binding agent) and carrier is added in proportion respectively in nutrient solution, inoculation 10
6individual spore/ml conidial suspension, 25 ± 1 DEG C, 180r/min shaken cultivation 16h, sampling microscopy germination rate (spore rate of living).Judge the impact of each auxiliary agent with germination rate height, the spore that germ tube length is equal to or greater than spore diameter is considered as sprouting, with spore fluid suspension in contrast.
The quality standard of described preparation is that Parasitism broom bacterium conidium dried bean noodles hangs in agent spore rate >=80.00% (Fig. 1) of living, moisture≤1.63%, pH value is 6.5, spore suspension rate >=82.12%, wetting time is 15s, and disintegration time is 78s, the granularity < of 90% 25 μm, particle mean size is 10.84 μm (Fig. 2), heat storage stability 30 DEG C, 60d; For qualified Parasitism broom bacterium dry suspending agent.
Embodiment 9
The application of Parasitism broom bacterium dry suspending agent
1 materials and methods
1.1 for examination material
1.1.1 Botryosphaeria berengeriana f. sp (PhysalosporapiricolaNose)
1.1.2 Sclerotinia sclerotiorum (Sclerotiniasclerotiorum)
1.1.3 reagent agent
The dry outstanding agent of present embodiment is 5% Parasitism broom bacterium conidial powder dry suspending agent, 20% Parasitism broom bacterium conidial powder dry suspending agent and 25% Parasitism broom bacterium conidial powder dry suspending agent, and contrast is respectively 70% tpn wetting powder and 25% Fluoxastrobin aqueous suspension agent.
1.2 method
Pot experiment: respectively various pathogen is gone to PDA flat board activation 3d, then go in PD liquid nutrient medium, every bottle turns 5 pieces, cultivate 6d, beat mycelia, survey light transmittance spray inoculation, moisturizing cultivates cucumber seedling to morbidity, and three kinds of pathogen light transmittances are respectively 1.3%, 3.6% and 9.8%; Parasitism broom bacterium dry suspending agent is taked respectively dilution 400 times, 800 times and 1600 times of liquid, spraying control pathogen, checks control efficiency after 15d, according to the sick level index criteria for classifying (table 1), according to following formulae discovery control efficiency.
The sick grade index criteria for classifying of table 1
Disease index=(∑ (sick value of series × this sick level number of sheets) × 100)/(sick level peak × investigation number of sheets)
Control efficiency (%)=(CK disease index-process disease index) × 100/CK disease index
1.3 results and analysis
Table 2 Parasitism broom bacterium conidial powder dry suspending agent is to the isolated test of ring rot of apple
Table 3 Parasitism broom bacterium conidial powder dry suspending agent is to the isolated test of rape sclerotium
The isolated test of the Parasitism broom bacterium conidial powder dry suspending agent of spray technique is adopted to the results are shown in Table 2,6.Known, this water dispersible granules to the preventive effect of ring rot of apple more than 75%, to sclerotinia rot of colza preventive effect more than 65%.
Table 4 Parasitism broom bacterium conidial powder dry suspending agent is to the pot experiment of ring rot of apple, sclerotinia rot of colza
Note: otherness (P=0.01), in table, digital back capitalization, represents significance in 99% level.
As shown in Table 4, the preventive effect of Parasitism broom bacterium Parasitism broom bacterium conidial powder dry suspending agent to two Plants diseases is different.Parasitism broom bacterium conidial powder dry suspending agent is to ring rot of apple > sclerotinia rot of colza, though the preventive effect of tested Parasitism broom bacterium conidial powder dry suspending agent is not as good as chemical pesticide Fluoxastrobin, but the preventive effect of 400 times of liquid to ring rot of apple disease also reaches 88%, and with Fluoxastrobin preventive effect difference pole not significantly (P > 0.01), though poorer than chemical pesticide to the preventive effect of sclerotinia rot of colza, but also reach more than 75%, and Parasitism broom bacterium conidial powder dry suspending agent microorganism belonging to genus agricultural chemicals category, without the 3R phenomenon of chemical pesticide, it is the agricultural chemicals of environment-friendly and green.
Claims (3)
1. a Parasitism broom bacterium conidium dried bean noodles suspension bactericide, it is characterized in that, it is as follows that formula presses mass percent proportioning: the mixture 12% of Parasitism broom bacterium conidial powder 5%, MorwetEFW and calcium lignosulfonate, crospolyvinylpyrrolidone 3%, nano zine oxide 0.2%, PEG4% and white carbon 75.8%, wherein, MorwetEFW is 1: 5 with the mixture quality ratio of calcium lignosulfonate; Described Parasitism broom bacterium conidial powder spore content>=1 × 10
10individual/g.
2. a Parasitism broom bacterium conidium dried bean noodles suspension bactericide, it is characterized in that, it is as follows that formula presses mass percent proportioning: Parasitism broom bacterium conidial powder 20%, the mixture 15% of TERWET1010 and TERSPERSE2700, PVPP 4%, vitamin C 0.3%, sodium lignin sulfonate 5% and diatomite 55.7%, wherein the mixture quality ratio of TERWET1010 and TERSPERSE2700 is 1: 4; Described Parasitism broom bacterium conidial powder spore content>=1 × 10
10individual/g.
3. a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide according to any one of claim 1-2 is as the application of disease of the control pathogenic ring rot of apple of agricultural and sclerotinia rot of colza.
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| CN105462873A (en) * | 2015-11-17 | 2016-04-06 | 北京克劳沃草业技术开发中心 | Method for making alfalfa rhizobium inoculant and rhizobium inoculant made through same and application thereof |
| CN105519584A (en) * | 2016-01-12 | 2016-04-27 | 郑州曹氏天正生物技术有限公司 | Freeze-dried powdered, dried and suspended fungicide and preparation method thereof |
| CN105707126B (en) * | 2016-03-29 | 2019-07-05 | 陕西先农生物科技有限公司 | A kind of bactericidal composition, preparation and its application |
| CN115316402A (en) * | 2022-07-29 | 2022-11-11 | 湖北省生物农药工程研究中心 | Trichoderma and dry suspension bactericide and preparation method thereof |
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