CN106342900B - A kind of Streptomyces jingyangensis wettable powder and preparation method thereof - Google Patents
A kind of Streptomyces jingyangensis wettable powder and preparation method thereof Download PDFInfo
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- CN106342900B CN106342900B CN201610733594.6A CN201610733594A CN106342900B CN 106342900 B CN106342900 B CN 106342900B CN 201610733594 A CN201610733594 A CN 201610733594A CN 106342900 B CN106342900 B CN 106342900B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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Abstract
The present invention provides a kind of preparation method of Streptomyces jingyangensis wettable powder, using kaolin as carrier, with Rhodopseudomonas palustris, cornstarch and bean cake powder are as high concentration Streptomyces jingyangensis bacterium powder made of nutritional agents fermentation, by 90-95 parts of Streptomyces jingyangensis zymophyte powder；Wetting agent: 1-4 parts of neopelex；Dispersing agent: 0.2-1 parts of Tea Saponin；Uv-protector: 0.5-2 parts of ascorbic acid, 0.2-1 parts of powder-beta-dextrin；Drying protectant: 1-4 parts of maltose, the ratio that 0.2-1 parts of mannitol is made a kind of stable, and bacterial content is more than the Streptomyces jingyangensis wettable powder of 10,000,000,000 CFU/g.
Description
Technical field
The present invention relates to a kind of preparation methods of Streptomyces jingyangensis wettable powder, belong to agriculture microorganism and biological pesticide
Technical field.
Background technique
Streptomyces jingyangensis (Streptomycesjingyangensis) is a kind of actinomyces, is commonly called as 5406.Streptomyces jingyangensis
Metabolic activity organic matter can be promoted to decompose, make nutrient validation, while antibacterial material can be discharged, it is more so as to be effectively prevented
Kind crop disease and insect, and the hormone of stimulation plant growth can be generated, it can also promote the formation of soil agreegate, have and promote corruption
It grows the formation of matter and generates the ability of polysaccharide.This antibiotic bacteria is widely used in multiple kinds of crops.Streptomycete is most raw
The actinomyces of anti-value, many kinds in streptomyces have antagonism to phytopathogen.More than 10 kinds have been filtered out at present
The most streptomycete of biological and ecological methods to prevent plant disease, pests, and erosion value.Streptomyces jingyangensis is to a variety of plants such as verticillium wilt pathogen, wilt, rhizoctonia solani, da mping-off fungi
Object disease is resistant, and can promote plant growth.Streptomyces jingyangensis is one plant of excellent bio-feritlizer and biological pesticide bacterial strain,
With the appearance of the development of industrialized agriculture, especially drip irrigation system, existing bacterium powder is not suitable for using；Although Li Yaning etc. is special
It is disclosed in benefit number 201110375945.8 a kind of using diatomite as the wettable of the rose yellow streptomycete biological bactericide of matrix
Pulvis, component and contains are as follows: rose yellow streptomycete bacterial strain formula can 0116 brood body >=108It is a；Wetting dispersing agent 4%.Yan Zhen etc. with
The seed liquor of Stremptomyces roseoflavus Men-myco-93-63 is test material, and finding suitable wetting dispersing agent is lignin sulfonic acid
Sodium adds the suspensibility maximum of sample after this auxiliary agent up to 50.27%.99.97% 44 μm of sample fineness <, moisture are reduced < 1.5%,
Wetting time < 18s.The wettable powder of Stremptomyces roseoflavus Men-myco-93-63 viable bacteria is finally had detected to muskmelon in greenhouse
The field efficacy of powdery mildew.The result shows that control efficiency is up to 87%.
But the preparation method of the wettable powder of Streptomyces jingyangensis does not have been reported that also, and the culture and research of Streptomyces jingyangensis exist
Age in last century 60-70 is studied more, is largely to ferment in loam, but we have found through overtesting, Chinese big
Part soil passes through nearly more than 30 years heavy dressing chemical fertilizer, and under the status of Shaoshi organic fertilizer, the acquisition of loam is not with front
Just and stablize, we carry out fermentation test in the soil sample that national more than 20 a provinces take, as a result, it has been found that utilizing existing technique
After fermentation, the spore content of Streptomyces jingyangensis was not highest all having 2,000,000,000 CFU/g, and can be remaining a large amount of in loam fermentation
Pest, can not kill, if applying the propagation that can cause pest in healthy soil, in addition loam discomfort is fit to do wettable powder
Carrier.Thallus content is too low in the wettable powder of existing streptomycete.
Summary of the invention
To solve the above-mentioned problems, the object of the present invention is to provide a kind of thallus content height, the Jingyangs of stable processing technique
Streptomycete wettable powder and preparation method thereof, the Streptomyces jingyangensis wettable powder are made of following parts by weight: Jingyang chain
90-95 parts of mold fermentation bacterium powder；Wetting agent: 1-4 parts of neopelex；Dispersing agent: 0.2-1 parts of Tea Saponin；Ultraviolet guarantor
Shield agent: 0.5-2 parts of ascorbic acid, 0.2-1 parts of powder-beta-dextrin；Drying protectant: 1-4 parts of maltose, 0.2-1 parts of mannitol；It has
Preparation is made of suddenly the following steps:
One, the preparation of Streptomyces jingyangensis zymophyte powder
Culture presevation pipe is taken out, plate is drawn with Gause I solid medium and recovers, 30 DEG C are cultivated 4-5 days.Flat
Picking single bacterium colony is seeded in eggplant bottle on Gause I solid medium under plate, fills entire eggplant bottle, in the incubator
30 DEG C are cultivated 4-6 days, wait phage surfaces to have baby pink spore, 1L sterile water is washed down the lawn in eggplant bottle, will
Streptomyces jingyangensis under washing is inoculated in the 500L seed fermentation tank equipped with 350L Streptomyces jingyangensis fluid nutrient medium, initial pH
Value is 7.2, and temperature is 30 DEG C, and divulge information 1:1, opens stirring, revolving speed 200r/min；Culture 36-48 hours, becomes sticky to thallus
It is thick, there are a large amount of mycelia to generate, can be used as Streptomyces jingyangensis liquid spawn；
Streptomyces jingyangensis is seeded in solid fermentation culture medium with 10% inoculum concentration, mixes booth into fermentation pallet, Gu
Body fermentation medium with a thickness of 3-5cm, 28-32 DEG C culture 2-3 days, grow a large amount of white bacterium points to solid fermentation culture medium,
Spore content is more than 10,500,000,000 cfu/g, can be air-dried to water content lower than 4%；
Wherein, the proportion of the solid fermentation culture medium are as follows: kaolin: cornstarch: bean cake powder: Rhodopseudomonas palustris
Liquid=100:2:2:20；
Wherein, the Gause I culture medium: starch 20g, NaCl 0.5g, KNO3 1g, KH2PO43H2O 0.5g,
FeSO47H2O0.01g, MgSO47H2O 0.5g, agar 18g, water 1000ml；
Wherein, the Streptomyces jingyangensis fluid nutrient medium: 20 g/L of glucose, 20 g/L of bean cake powder, 5 g/L of sodium chloride,
0.5 g/L of magnesium sulfate, calcium carbonate 2 g/L, pH7.2；
The condition of each medium sterilization are as follows: 0.10-0.15MPa, 121 DEG C sterilize 30 minutes；
Wherein, the preparation of Rhodopseudomonas palustris bacterium solution: taking out Rhodopseudomonas palustris kind preservation pipe, red false single with marsh
Born of the same parents' bacterium solid medium draws plate and recovers, and cultivates 72 hours for 30 DEG C in illumination box.Intensity of illumination: 1000lx-
6000lx.Picking single bacterium colony is inoculated in 50 milliliters of Rhodopseudomonas palustris fluid nutrient mediums under plate, in illumination box
In 30 DEG C Anaerobic culturel 120 hours.Seed uses 20% inoculum concentration, is seeded to the Rhodopseudomonas palustris liquid training equipped with 4L
The big triangular flask of 5L of base is supported, its bacterial concentration is detected in 30 DEG C Anaerobic culturel 72-96 hours in illumination box, and bacterial content is super
3,000,000,000 CFU/ml are crossed, can be used as Rhodopseudomonas palustris bacterium solution.The wherein Rhodopseudomonas palustris solid medium: phosphoric acid
Potassium dihydrogen 1g, calcium chloride 0.1g, sodium carbonate 3g, sodium acetate 1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, agar 20g,
PH7.5, water 1000mL.The wherein Rhodopseudomonas palustris fluid nutrient medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, carbonic acid
Hydrogen sodium 2g, sodium acetate 4g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, microelement 10mL, pH7.5, water 1000mL.Respectively
The condition of medium sterilization are as follows: 0.10-0.15MPa, 121 DEG C sterilize 30 minutes；Wherein, the preparation of microelement: sulfuric acid monohydrate
4 g of manganese, boric acid 2 g, Sodium Molybdate Dihydrate 0.05g, cupric sulfate pentahydrate 0.005g, CoCL2 6H2O 0.05g, water 1000mL.
Two, by 90-95 parts of Streptomyces jingyangensis zymophyte powder；Wetting agent: 1-4 parts of neopelex；Dispersing agent: tea
0.2-1 parts of saponin；Uv-protector: 0.5-2 parts of ascorbic acid, 0.2-1 parts of powder-beta-dextrin；Drying protectant: 1-4 parts of maltose,
0.2-1 parts of mannitol；Mixing and crossing crushed 400 meshes by airslide disintegrating mill, detect its Streptomyces jingyangensis spore content not
Lower than 10,000,000,000 cfu/g, suspensibility is not less than 85%, and wetting time is not higher than 85s, and moisture content is lower than 5%, is vacuum-packed, as
Streptomyces jingyangensis wettable powder.
Wherein, Streptomyces jingyangensis wettable powder is preferentially made of following weight point are as follows: by Streptomyces jingyangensis zymophyte powder
90 parts；Wetting agent: 3 parts of neopelex；Dispersing agent: 0.7 part of Tea Saponin；Uv-protector: 1.5 parts of ascorbic acid,
0.6 part of powder-beta-dextrin；Drying protectant: 3 parts of maltose, 0.8 part of mannitol.
The preparation of Streptomyces jingyangensis yeast powder of the invention is using colony forming single-digit as index, to fermentation medium components
Carry out single factor test screening.Selection diatomite, bentonite, zeolite powder, kaolin, white carbon black, ground phosphate rock, precipitated calcium carbonate are first
Test matrix, the results showed that, which can grow in the above matrix, but the upgrowth situation in kaolin is more excellent, therefore really
Determining kaolin is suitable solid matrix.By optimizing carbon nitrogen source (cornstarch, glucose, soluble starch, flour, cassava
Powder, peptone, yeast extract, bean cake powder, cotton dregs powder, Dried Corn Steep Liquor Powder, sodium nitrate, ammonium chloride, ammonium sulfate) and the life of compound dimension
Element, composite trace element, Rhodopseudomonas palustris liquid etc., discovery are kaolin in carrier, and carbon source is cornstarch, and nitrogen source is beans
Dregs of rice powder, the especially creative Rhodopseudomonas palustris liquid of addition in the medium contain in Rhodopseudomonas palustris fermentation liquid
Several physiological active substances, such as chlorophyll A, B, carotenoid, multivitamin (especially B family vitamin), Duo Zhongsheng
Long hormone (such as IAA, GA3, ABA, ethylene, the basic element of cell division), nucleic acid, salicylic acid, a variety of amino acid and single cell protein
Deng, it can also be grown for Streptomyces jingyangensis and provide certain unknown trophic factors, and make its Streptomyces jingyangensis spore content significantly
It improves, improves similar 2 times or more, can be more than 12,000,000,000 CFU/ grams, the content of the streptomycete spore substantially increased,
The present inventor by auxiliary agent (wetting agent and dispersing agent) (polyvinyl alcohol, polyethylene glycol, CMC-Na, Tween-60,
Neopelex, T-type lignin, lauryl sodium sulfate, Tea Saponin, Morwet EFW, Morwet D425,
My100, D110, Arab's power glue, 2- naphthalene sulfonic acid formaldehyde polymer sodium salt, octyl phenol polyoxyethylene ether, diisooctyl succinic acid
Ester sodium sulfonate, methylcellulose, sodium lignin sulfonate), ultraviolet protective agent (ascorbic acid, dextrin, dodecyl benzene sulfonic acid
Sodium, Congo red, carboxymethyl cellulose, lecithin), drying protectant (skimmed milk power, sucrose, sodium glutamate, maltose, sweet dew
Alcohol, methylcellulose etc.) it is screened, every kind of auxiliary agent survives to the spore of Streptomyces jingyangensis, and suspension, wettability is tried
It tests, the formula of Streptomyces jingyangensis wettable powder is determined finally by more wheel single factor tests and orthogonal test.Detect its Jingyang strepto-
Bacterium spore content is not less than 10,000,000,000 cfu/g, and suspensibility is not less than 85%, and wetting time is not higher than 85s, and moisture content is lower than 5%,
By verifying, Streptomyces jingyangensis wettable powder of the invention saves 1 year at 20-25 DEG C, and Conidia persistence also can reach
85%。
Compared with prior art, the present invention having the following advantages that and effect:
Creative is introduced into Rhodopseudomonas palustris liquid in Streptomyces jingyangensis culture medium, mentions its spore content significantly
Height improves 2 times or more than original formula, has reached 12,000,000,000 CFU/ grams or more.
Provide it is a kind of prepare Streptomyces jingyangensis wettable powder, Streptomyces jingyangensis spore content is not less than 10,000,000,000 cfu/
G, finess disintegrating cross 400 meshes, and suspensibility is not less than 85%, and wetting time is not higher than 85s, and moisture content is lower than 5% preparation side
Method, preparation method are stablized, and usage amount is few, and effect is good.
Specific embodiment
Embodiment 1
A kind of Streptomyces jingyangensis wettable powder, which is characterized in that the Streptomyces jingyangensis wettable powder is by following heavy
It measures part to form: 90 parts of Streptomyces jingyangensis zymophyte powder；Wetting agent: 3 parts of neopelex；Dispersing agent: Tea Saponin 0.7
Part；Uv-protector: 1.5 parts of ascorbic acid, 0.6 part of powder-beta-dextrin；Drying protectant: 3 parts of maltose, 0.8 part of mannitol；It has
Preparation is made of suddenly the following steps:
One, the preparation of Streptomyces jingyangensis zymophyte powder
Culture presevation pipe is taken out, plate is drawn with Gause I solid medium and recovers, 30 DEG C are cultivated 4-5 days, flat
Picking single bacterium colony is seeded in eggplant bottle on Gause I solid medium under plate, fills entire eggplant bottle, in the incubator
30 DEG C are cultivated 4-6 days, wait phage surfaces to have baby pink spore, 1L sterile water is washed down the lawn in eggplant bottle, will
Streptomyces jingyangensis under washing is inoculated in the 500L seed fermentation tank equipped with 350L Streptomyces jingyangensis fluid nutrient medium, initial pH
Value is 7.2, and temperature is 30 DEG C, and divulge information 1:1, opens stirring, revolving speed 200r/min；Culture 36-48 hours, becomes sticky to thallus
It is thick, there are a large amount of mycelia to generate, can be used as Streptomyces jingyangensis liquid spawn；
Streptomyces jingyangensis liquid spawn is seeded in solid fermentation culture medium with 10% inoculum concentration, mixes booth to fermentation
In pallet, solid fermentation culture medium with a thickness of 3-5cm, 28-32 DEG C is cultivated 3 days, is grown to solid fermentation culture medium a large amount of white
Color bacterium point, detection spore content are 11,200,000,000 cfu/g, can be air-dried to water content lower than 4%；
Wherein, the proportion of the solid fermentation culture medium are as follows: kaolin: cornstarch: bean cake powder: Rhodopseudomonas palustris
Liquid=100:2:2:20；
Wherein, the Gause I culture medium: starch 20g, NaCl 0.5g, KNO3 1g, KH2PO43H2O 0.5g,
FeSO47H2O0.01g, MgSO47H2O 0.5g, agar 18g, water 1000ml；
Wherein, the Streptomyces jingyangensis fluid nutrient medium: 20 g/L of glucose, 20 g/L of bean cake powder, 5 g/L of sodium chloride,
0.5 g/L of magnesium sulfate, calcium carbonate 2 g/L, pH7.2；The condition of each medium sterilization are as follows: 0.10-0.15MPa, 121 DEG C of sterilizings 30
Minute；
Wherein, the preparation of Rhodopseudomonas palustris bacterium solution: taking out Rhodopseudomonas palustris kind preservation pipe, red false single with marsh
Born of the same parents' bacterium solid medium draws plate and recovers, and cultivates 72 hours for 30 DEG C in illumination box, intensity of illumination: 1000lx-
6000lx, picking single bacterium colony is inoculated in 50 milliliters of Rhodopseudomonas palustris fluid nutrient mediums under plate, in illumination box
In 30 DEG C Anaerobic culturel 120 hours, seed use 20% inoculum concentration, be seeded to equipped with 4L Rhodopseudomonas palustris liquid training
The big triangular flask of 5L of base is supported, its bacterial concentration is detected in 30 DEG C Anaerobic culturel 72-96 hours in illumination box, and bacterial content is super
3,000,000,000 CFU/ml are crossed, can be used as Rhodopseudomonas palustris bacterium solution；
Wherein, the Rhodopseudomonas palustris solid medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, sodium carbonate 3g, vinegar
Sour sodium 1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, agar 20g, pH7.5, water 1000mL；
Wherein, the Rhodopseudomonas palustris fluid nutrient medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, sodium bicarbonate 2g,
Sodium acetate 4g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, microelement 10mL, pH7.5, water 1000mL；Each culture medium goes out
The condition of bacterium are as follows: 0.10-0.15MPa, 121 DEG C sterilize 30 minutes；
Wherein, the preparation of microelement: 4 g of manganese sulfate monohydrate, 2 g of boric acid, Sodium Molybdate Dihydrate 0.05g, five water sulfuric acid
Copper 0.005g, CoCL2 6H2O 0.05g, water 1000mL；
Two, by 90 parts of Streptomyces jingyangensis zymophyte powder；Wetting agent: 3 parts of neopelex；Dispersing agent: Tea Saponin
0.7 part；Uv-protector: 1.5 parts of ascorbic acid, 0.6 part of powder-beta-dextrin；Drying protectant: 3 parts of maltose, 0.8 part of mannitol；
Mixing and crossing crushed 400 meshes by airslide disintegrating mill, and detecting its Streptomyces jingyangensis spore content is 10,400,000,000 cfu/g, suspend
Rate is 89%, wetting time 60s, moisture content 4%, vacuum packaging, as Streptomyces jingyangensis wettable powder.
Embodiment 2
The application effect of prevention and treatment of the Streptomyces jingyangensis wettable powder of the present invention in banana blight
1 test period: in June, -2016 in May, 2014
2. test site: Guangdong Province Xuwen County farm
3. testing program: test group: monthly trickle irrigation is primary, and dosage is Jingyang chain streptomycete wettable powder 1 of the invention
Kg/acre, usable floor area is 22 mu；Control group: certain commercially available 10 kilograms of bacillus wettable powder (banana is dedicated) of use/
Mu, usable floor area are 108 mu, and control group, in March, 2015, is opened using bacillus wettable powder use from April, 2015
Begin also to use Streptomyces jingyangensis wettable powder of the invention；Other management are identical.
4. test result:
We have counted test group and control group incidence, as a result such as the following table 1, it can clearly be seen that test from table 1
Group uses the disease incidence of Streptomyces jingyangensis wettable powder of the invention over time, and disease incidence is lower and lower, until
In June, 2016, disease incidence was controlled to 1% or less；And control group is in the bacillus wettable powder of application (banana is dedicated)
Its disease incidence is not controlled significantly, and there are also increasingly severe trend, and plantation master is caused to change privately in April, 2015
Streptomyces jingyangensis wettable powder of the invention is all used in all soils by testing program, is just able to significantly control disease
Feelings.
Control efficiency of the 1 Streptomyces jingyangensis wettable powder of table to banana blight
| Disease incidence/time | In May, 2014 | In December, 2014 | In March, 2015 | In June, 2015 | In December, 2015 | In June, 2016 |
| Test group | 15.8% | 12.6% | 10.3% | 7.4% | 3.3% | 0.9% |
| Control group | 12.4 | 14.4% | 16.6% | 15.2% | 10.1% | 5.6% |
Claims (1)
1. a kind of Streptomyces jingyangensis wettable powder, which is characterized in that the Streptomyces jingyangensis wettable powder is by following weight
Part composition: 90 parts of Streptomyces jingyangensis zymophyte powder；Wetting agent: 3 parts of neopelex；Dispersing agent: 0.7 part of Tea Saponin；
Uv-protector: 1.5 parts of ascorbic acid, 0.6 part of powder-beta-dextrin；Drying protectant: 3 parts of maltose, 0.8 part of mannitol；It is specific
Preparation method is made of suddenly the following steps:
One, the preparation of Streptomyces jingyangensis zymophyte powder
Culture presevation pipe is taken out, plate is drawn with Gause I solid medium and recovers, 30 DEG C are cultivated 4-5 days, under plate
Picking single bacterium colony is seeded in eggplant bottle on Gause I solid medium, fills entire eggplant bottle, and 30 DEG C in the incubator
Culture 4-6 days, waits phage surfaces to have baby pink spore, the lawn in eggplant bottle is washed down by 1L sterile water, will be washed down
Streptomyces jingyangensis be inoculated in the 500L seed fermentation tank equipped with 350L Streptomyces jingyangensis fluid nutrient medium, initial pH value is
7.2, temperature is 30 DEG C, and divulge information 1:1, opens stirring, revolving speed 200r/min；Culture 36-48 hours, becomes viscous to thallus, has
A large amount of mycelia generate, and can be used as Streptomyces jingyangensis liquid spawn；
Streptomyces jingyangensis liquid spawn is seeded in solid fermentation culture medium with 10% inoculum concentration, mixes booth to fermentation pallet
In, solid fermentation culture medium with a thickness of 3-5cm, 28-32 DEG C is cultivated 3 days, grows a large amount of white bacterium to solid fermentation culture medium
Point, detection spore content are 11,200,000,000 cfu/g, can be air-dried to water content lower than 4%；
Wherein, the proportion of the solid fermentation culture medium are as follows: kaolin: cornstarch: bean cake powder: Rhodopseudomonas palustris liquid=
100:2:2:20；
Wherein, the Gause I culture medium: starch 20g, NaCl 0.5g, KNO3 1g, KH2PO43H2O 0.5g,
FeSO47H2O0.01g, MgSO47H2O 0.5g, agar 18g, water 1000ml；
Wherein, the Streptomyces jingyangensis fluid nutrient medium: 20 g/L of glucose, 20 g/L of bean cake powder, 5 g/L of sodium chloride, sulfuric acid
0.5 g/L of magnesium, calcium carbonate 2 g/L, pH7.2；The condition of each medium sterilization are as follows: 0.10-0.15MPa, 121 DEG C sterilize 30 points
Clock；
Wherein, the preparation of Rhodopseudomonas palustris bacterium solution: Rhodopseudomonas palustris kind preservation pipe is taken out, Rhodopseudomonas palustris is used
Solid medium draws plate and recovers, and cultivates 72 hours for 30 DEG C in illumination box, intensity of illumination: 1000lx-6000lx,
Picking single bacterium colony is inoculated in 50 milliliters of Rhodopseudomonas palustris fluid nutrient mediums under plate, is detested for 30 DEG C in illumination box
Oxygen culture 120 hours, seed used 20% inoculum concentration, was seeded to the 5L of the Rhodopseudomonas palustris fluid nutrient medium equipped with 4L
Its bacterial concentration is detected in big triangular flask, 30 DEG C Anaerobic culturel 72-96 hours in illumination box, and bacterial content is more than 3,000,000,000
CFU/ml can be used as Rhodopseudomonas palustris bacterium solution；
Wherein, the Rhodopseudomonas palustris solid medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, sodium carbonate 3g, sodium acetate
1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, agar 20g, pH7.5, water 1000mL；
Wherein, the Rhodopseudomonas palustris fluid nutrient medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, sodium bicarbonate 2g, acetic acid
Sodium 4g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, microelement 10mL, pH7.5, water 1000mL；Each medium sterilization
Condition are as follows: 0.10-0.15MPa, 121 DEG C sterilize 30 minutes；
Wherein, the preparation of microelement: 4 g of manganese sulfate monohydrate, boric acid 2 g, Sodium Molybdate Dihydrate 0.05g, cupric sulfate pentahydrate
0.005g, CoCL2 6H2O 0.05g, water 1000mL；
Two, by 90 parts of Streptomyces jingyangensis zymophyte powder；Wetting agent: 3 parts of neopelex；Dispersing agent: Tea Saponin 0.7
Part；Uv-protector: 1.5 parts of ascorbic acid, 0.6 part of powder-beta-dextrin；Drying protectant: 3 parts of maltose, 0.8 part of mannitol；It mixes
And cross and 400 meshes crushed by airslide disintegrating mill, detecting its Streptomyces jingyangensis spore content is 10,400,000,000 cfu/g, and suspensibility is
89%, wetting time 60s, moisture content 4%, vacuum packaging, as Streptomyces jingyangensis wettable powder.
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