CN106342900A - Streptomyces jingyangensis wettable powder and preparation method thereof - Google Patents
Streptomyces jingyangensis wettable powder and preparation method thereof Download PDFInfo
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- CN106342900A CN106342900A CN201610733594.6A CN201610733594A CN106342900A CN 106342900 A CN106342900 A CN 106342900A CN 201610733594 A CN201610733594 A CN 201610733594A CN 106342900 A CN106342900 A CN 106342900A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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Abstract
The invention provides a preparation method of Streptomyces jingyangensis wettable powder. The method comprises the following steps: fermenting a carrier kaolin, Rhodopseudomonas palustris, and nutrients corn starch and bean pulp powder to obtain high-concentration Streptomyces jingyangensis powder; and mixing 90-95 parts of Streptomyces jingyangensis fermented powder, 1-4 parts of wetting agent sodium dodecyl benzene sulfonate, 0.2-1 part of dispersing agent tea saponin, 0.5-2 parts of ultraviolet protective agent ascorbic acid, 0.2-1 part of beta-dextrin, 1-4 parts of drying protective agent maltose and 0.2-1 part of mannitol to obtain the stable Streptomyces jingyangensis wettable powder with the bacterium content of more than 10 billion CFU/g.
Description
Technical field
The present invention relates to a kind of preparation method of Streptomyces jingyangensis wettable powder, belong to agriculture microorganism and biological pesticide
Technical field.
Background technology
Streptomyces jingyangensis (streptomycesjingyangensis) are a kind of actinomycetes, are commonly called as 5406.Streptomyces jingyangensis
Metabolic activity can promote Organic substance decompose, make nutrient validation, antibiotic substance can be discharged simultaneously, many so as to be effectively prevented
Plant crop disease and insect, and the hormone stimulating plant growing can be produced, it can also promote the formation of soil agreegate, there is promotion rotten
Grow the formation of matter and the ability producing polysaccharide.This antibiotic bacteria widely uses in multiple kinds of crops.Streptomycete is most to give birth to
The actinomycetes of anti-value, the many kind in streptomyces has antagonism to phytopathogen.Filter out kind more than 10 at present
The streptomycete that most biological and ecological methods to prevent plant disease, pests, and erosion is worth.Streptomyces jingyangensis to verticillium wilt pathogen, wilt, rhizoctonia solani, da mping-off fungi etc. more plants
Thing disease has resistance, and can promote plant growing.Streptomyces jingyangensis are one plant of excellent bio-feritlizer and biological pesticide bacterial strain,
With the development of industrialized agriculture, the particularly appearance of drip irrigation system, existing mycopowder is not suitable for using；Although Li Yaning etc. is special
A kind of wettability of the rose yellow streptomycete biofungicide with kieselguhr as substrate is disclosed in profit number 201110375945.8
Powder, its component and containing being: rose yellow streptomycete bacterial strain formula can 0116 brood body >=108Individual；Wetting dispersing agent 4%.Yan Zhen etc. with
The seed liquor of rose yellow streptomycete men-myco-93-63 is test material, and finding suitable wetting dispersing agent is lignin sulfonic acid
Sodium, add sample after this auxiliary agent suspensibility maximum up to 50.27%.99.97% 44 μm of sample fineness ＜, moisture decrement ＜ 1.5%,
Wetting time ＜ 18s.The wettable powder that finally have detected rose yellow streptomycete men-myco-93-63 viable bacteria is to Fructus Melo in booth
The field efficacy of powdery mildew.Result shows, its prevention effect is up to 87%.
But the preparation method of the wettable powder of Streptomyces jingyangensis does not also have been reported that, the culture of Streptomyces jingyangensis is existed with research
Age in last century 60-70 is studied more, and major part is to be fermented in loam, but we find through overtesting, big in China
Through the heavy dressing chemical fertilizer of nearly more than 30 years, under the present situation of Shaoshi fertilizer, the acquisition of loam did not had with front part soil
Just and stable, the soil sample that we take in more than 20, whole nation province carries out fermentation test, it is found that utilizing existing technique
After fermentation, the spore content highest of Streptomyces jingyangensis did not all have 2,000,000,000 cfu/g, and can be remaining a large amount of in loam fermentation
Insect it is impossible to kill, if applying the propagation that can cause insect in healthy soil, in addition loam discomfort is fit to do wettable powder
Carrier.In the wettable powder of existing streptomycete, thalline content is too low.
Content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of thalline content is high, the Jingyang of stable processing technique
Streptomycete wettable powder and preparation method thereof, described Streptomyces jingyangensis wettable powder is made up of following weight portion: Jingyang chain
Mold fermentation mycopowder 90-95 part；Wetting agent: dodecylbenzene sodium sulfonate 1-4 part；Dispersant: tea saponin 0.2-1 part；Ultraviolet is protected
Shield agent: ascorbic acid 0.5-2 part, powder-beta-dextrin 0.2-1 part；Drying protectant: maltose 1-4 part, Mannitol 0.2-1 part；Its tool
Preparation, is made up of suddenly the following steps:
First, the preparation of Streptomyces jingyangensis zymophyte powder
Take out culture presevation pipe, draw flat board with Gause I solid medium and recovered, cultivate 4-5 days for 30 DEG C.Under flat board
Picking single bacterium colony is seeded on Gause I solid medium in Fructus Solani melongenae bottle, fills whole Fructus Solani melongenae bottle, 30 DEG C in incubator
Culture 4-6 days, waits phage surface to have baby pink spore, the lawn in Fructus Solani melongenae bottle is washed down by 1l sterilized water, will wash down
Streptomyces jingyangensis be inoculated in 500l seed fermentation tank equipped with 350l Streptomyces jingyangensis fluid medium, starting ph is
7.2, temperature is 30 DEG C, and divulge information 1:1, opens stirring, and rotating speed is 200r/min；Culture 36-48 hour, treats that thalline becomes viscous, has
A large amount of mycelia produce, you can as Streptomyces jingyangensis liquid spawn；
Streptomyces jingyangensis are seeded in solid fermentation culture medium with 10% inoculum concentration, mix stand to fermenting in pallet, solid is sent out
The thickness of ferment culture medium is 3-5cm, cultivates 2-3 days for 28-32 DEG C, treats that solid fermentation culture medium grows white bacterium point in a large number, spore
Content is more than 10,500,000,000 cfu/g, you can air-dries and is less than 4% to water content；
Wherein, the proportioning of described solid fermentation culture medium is: Kaolin: corn starch: bean cake powder: Rhodopseudomonas palustris liquid=
100:2:2:20；
Wherein, described Gause I culture medium: starch 20g, nacl 0.5g, kno3 1g, kh2po4 3h2o 0.5g,
Feso4 7h2o0.01g, mgso4 7h2o 0.5g, agar 18g, water 1000ml；
Wherein, described Streptomyces jingyangensis fluid medium: glucose 20 g/l, bean cake powder 20 g/l, sodium chloride 5 g/l, sulphuric acid
Magnesium 0.5 g/l, Calcium Carbonate 2 g/l, ph7.2；
The condition of each medium sterilization is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes；
Wherein, the preparation of Rhodopseudomonas palustris bacterium solution: take out Rhodopseudomonas palustris kind preservation pipe, use Rhodopseudomonas palustris
Solid medium is drawn flat board and is recovered, and cultivates 72 hours for 30 DEG C in illumination box.Intensity of illumination: 1000lex-
6000lex.Under flat board, picking single bacterium colony is inoculated in 50 milliliters of Rhodopseudomonas palustris fluid mediums, in illumination box
In 30 DEG C of Anaerobic culturel 120 hours.Seed adopts 20% inoculum concentration, is seeded to the Rhodopseudomonas palustris liquid training equipped with 4l
The big triangular flask of 5l of foster base, in illumination box, 30 DEG C of Anaerobic culturel 72-96 hours, detect its bacterial concentration, and bacterial content surpasses
Cross 3,000,000,000 cfu/ml, you can as Rhodopseudomonas palustris bacterium solution.Wherein said Rhodopseudomonas palustris solid medium: phosphoric acid
Potassium dihydrogen 1g, calcium chloride 0.1g, sodium carbonate 3g, Sodium Acetate Trihydrate 1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, agar 20g,
Ph7.5, water 1000ml.Wherein said Rhodopseudomonas palustris fluid medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, carbonic acid
Hydrogen sodium 2g, Sodium Acetate Trihydrate 4g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, trace element 10ml, ph7.5, water 1000ml.Respectively
The condition of medium sterilization is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes；Wherein, the preparation of trace element: sulfuric acid monohydrate
Manganese 4 g, boric acid 2 g, Sodium Molybdate Dihydrate 0.05g, copper sulphate pentahydrate 0.005g, cobalt chloride hexahydrate 0.05g, water 1000ml.
2nd, by Streptomyces jingyangensis zymophyte powder 90-95 part；Wetting agent: dodecylbenzene sodium sulfonate 1-4 part；Dispersant: tea
Saponin 0.2-1 part；Uv-protector: ascorbic acid 0.5-2 part, powder-beta-dextrin 0.2-1 part；Drying protectant: maltose 1-4 part,
Mannitol 0.2-1 part；Mix and cross and 400 mesh sieves were pulverized by jet mill, detect its Streptomyces jingyangensis spore content not
Less than 10,000,000,000 cfu/g, suspensibility is not less than 85%, and wetting time is not higher than 85s, and moisture is less than 5%, is vacuum-packed, as
Streptomyces jingyangensis wettable powder.
Wherein, Streptomyces jingyangensis wettable powder is divided by following weight and preferentially consists of: by Streptomyces jingyangensis zymophyte powder
90 parts；Wetting agent: 3 parts of dodecylbenzene sodium sulfonate；Dispersant: 0.7 part of tea saponin；Uv-protector: 1.5 parts of ascorbic acid,
0.6 part of powder-beta-dextrin；Drying protectant: 3 parts of maltose, 0.8 part of Mannitol.
The preparation of the Streptomyces jingyangensis yeast powder of the present invention with colony forming single-digit as index, to fermentation medium components
Carry out single factor test screening.Select kieselguhr, bentonite, zeolite powder, Kaolin, white carbon, ground phosphate rock first, precipitated calcium carbonate is
Test matrix, result shows, this bacterium all can grow in above substrate, but the upgrowth situation in Kaolin is more excellent, therefore really
Determining Kaolin is suitable solid matrix.By optimizing carbon nitrogen source (corn starch, glucose, soluble starch, flour, Maninot esculenta crantz.
Powder, peptone, yeast extract, bean cake powder, cottonseed meal powder, Dried Corn Steep Liquor Powder, sodium nitrate, ammonium chloride, ammonium sulfate), and compound dimension life
Element, compound microelement, Rhodopseudomonas palustris liquid etc., find in carrier to be Kaolin, carbon source is corn starch, nitrogen source is bean
Dregs of rice powder, the particularly Rhodopseudomonas palustris liquid of addition in the medium of creativeness, contains in Rhodopseudomonas palustris fermentation liquid
Several physiological active substances, such as chlorophyll a, b, carotenoid, multivitamin (especially b vitamin), Duo Zhongsheng
Long hormone (as iaa, ga3, aba, ethylene, the basic element of cell division), nucleic acid, salicylic acid, several amino acids and SCP
Deng also some unknown trophic factors can be provided for Streptomyces jingyangensis growth, and make its Streptomyces jingyangensis spore content significantly
Improve, improve similar more than 2 times, 12,000,000,000 cfu/ gram can be exceeded, the content of the streptomycete spore substantially increasing,
The present inventor by auxiliary agent (wetting agent and dispersant) (polyvinyl alcohol, Polyethylene Glycol, cmc-na, Tween-60,12
Sodium alkyl benzene sulfonate, t type lignin, sodium lauryl sulphate, tea saponin, morwet efw, morwet d425, my100,
D110, Arabic power glue, 2- naphthalene sulfonic acid formaldehyde polymer sodium salt, OPEO, diisooctyl succinate sulfonic acid
Sodium, methylcellulose, sodium lignin sulfonate), ultraviolet protective agent (ascorbic acid, dextrin, dodecylbenzene sodium sulfonate, the Congo
Red, carboxymethyl cellulose, lecithin), drying protectant (defatted milk powder, sucrose, sodium glutamate, maltose, Mannitol, methyl
Cellulose etc.) screened, its every kind of auxiliary agent is survived to the spore of Streptomyces jingyangensis, suspension, and wettability is tested, finally
Determine the formula of Streptomyces jingyangensis wettable powder by many wheel single factor test and orthogonal test.Detect that its Streptomyces jingyangensis spore contains
Amount is not less than 10,000,000,000 cfu/g, and suspensibility is not less than 85%, and wetting time is not higher than 85s, and moisture is less than 5%, by checking,
The Streptomyces jingyangensis wettable powder of the present invention preserves 1 year at 20-25 DEG C, and its Conidia persistence also can reach 85%.
The present invention compared with prior art, has advantages below and an effect:
Creative Rhodopseudomonas palustris liquid is introduced into so as to spore content greatly improves in Streptomyces jingyangensis culture medium, than
Formula originally improves more than 2 times, has reached 12,000,000,000 more than cfu/ gram.
Provide one kind and prepare Streptomyces jingyangensis wettable powder, Streptomyces jingyangensis spore content is not less than 10,000,000,000 cfu/
G, finess disintegrating crosses 400 mesh sieves, and suspensibility is not less than 85%, and wetting time is not higher than 85s, and moisture is less than 5% preparation side
Method, preparation method is stable, and usage amount is few, and effect is good.
Specific embodiment
Embodiment 1
A kind of Streptomyces jingyangensis wettable powder is it is characterised in that described Streptomyces jingyangensis wettable powder is by following weight portion
Composition: 90 parts of Streptomyces jingyangensis zymophyte powder；Wetting agent: 3 parts of dodecylbenzene sodium sulfonate；Dispersant: 0.7 part of tea saponin；Purple
Outer protective agent: 1.5 parts of ascorbic acid, 0.6 part of powder-beta-dextrin；Drying protectant: 3 parts of maltose, 0.8 part of Mannitol；It is specifically made
Preparation Method, is made up of suddenly the following steps:
First, the preparation of Streptomyces jingyangensis zymophyte powder
Take out culture presevation pipe, draw flat board with Gause I solid medium and recovered, cultivate 4-5 days, under flat board for 30 DEG C
Picking single bacterium colony is seeded on Gause I solid medium in Fructus Solani melongenae bottle, fills whole Fructus Solani melongenae bottle, 30 DEG C in incubator
Culture 4-6 days, waits phage surface to have baby pink spore, the lawn in Fructus Solani melongenae bottle is washed down by 1l sterilized water, will wash down
Streptomyces jingyangensis be inoculated in 500l seed fermentation tank equipped with 350l Streptomyces jingyangensis fluid medium, starting ph is
7.2, temperature is 30 DEG C, and divulge information 1:1, opens stirring, and rotating speed is 200r/min；Culture 36-48 hour, treats that thalline becomes viscous, has
A large amount of mycelia produce, you can as Streptomyces jingyangensis liquid spawn；
Streptomyces jingyangensis liquid spawn is seeded in solid fermentation culture medium with 10% inoculum concentration, mixes stand to the pallet that ferments
In, the thickness of solid fermentation culture medium is 3-5cm, cultivates 3 days for 28-32 DEG C, treats that solid fermentation culture medium grows white bacterium in a large number
Point, detection spore content is 11,200,000,000 cfu/g, you can air-dries and is less than 4% to water content；
Wherein, the proportioning of described solid fermentation culture medium is: Kaolin: corn starch: bean cake powder: Rhodopseudomonas palustris liquid=
100:2:2:20；
Wherein, described Gause I culture medium: starch 20g, nacl 0.5g, kno3 1g, kh2po4 3h2o 0.5g,
Feso4 7h2o0.01g, mgso4 7h2o 0.5g, agar 18g, water 1000ml；
Wherein, described Streptomyces jingyangensis fluid medium: glucose 20 g/l, bean cake powder 20 g/l, sodium chloride 5 g/l, sulphuric acid
Magnesium 0.5 g/l, Calcium Carbonate 2 g/l, ph7.2；The condition of each medium sterilization is: 0.10-0.15mpa, and 121 DEG C sterilize 30 points
Clock；
Wherein, the preparation of Rhodopseudomonas palustris bacterium solution: take out Rhodopseudomonas palustris kind preservation pipe, use Rhodopseudomonas palustris
Solid medium is drawn flat board and is recovered, and cultivates 72 hours for 30 DEG C, intensity of illumination: 1000lex- in illumination box
6000lex, under flat board, picking single bacterium colony is inoculated in 50 milliliters of Rhodopseudomonas palustris fluid mediums, in illumination box
In 30 DEG C of Anaerobic culturel 120 hours, seed adopts 20% inoculum concentration, is seeded to the Rhodopseudomonas palustris liquid training equipped with 4l
The big triangular flask of 5l of foster base, in illumination box, 30 DEG C of Anaerobic culturel 72-96 hours, detect its bacterial concentration, and bacterial content surpasses
Cross 3,000,000,000 cfu/ml, you can as Rhodopseudomonas palustris bacterium solution；
Wherein, described Rhodopseudomonas palustris solid medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, sodium carbonate 3g, Sodium Acetate Trihydrate
1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, agar 20g, ph7.5, water 1000ml；
Wherein, described Rhodopseudomonas palustris fluid medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, sodium bicarbonate 2g, acetic acid
Sodium 4g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, trace element 10ml, ph7.5, water 1000ml；Each medium sterilization
Condition is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes；
Wherein, the preparation of trace element: manganese sulfate monohydrate 4 g, boric acid 2 g, Sodium Molybdate Dihydrate 0.05g, copper sulphate pentahydrate
0.005g, cobalt chloride hexahydrate 0.05g, water 1000ml；
2nd, by 90 parts of Streptomyces jingyangensis zymophyte powder；Wetting agent: 3 parts of dodecylbenzene sodium sulfonate；Dispersant: tea saponin 0.7
Part；Uv-protector: 1.5 parts of ascorbic acid, 0.6 part of powder-beta-dextrin；Drying protectant: 3 parts of maltose, 0.8 part of Mannitol；Mix
And excessively 400 mesh sieves were pulverized by jet mill, detect that its Streptomyces jingyangensis spore content is 10,400,000,000 cfu/g, suspensibility is
89%, wetting time is 60s, and moisture is 4%, vacuum packaging, as Streptomyces jingyangensis wettable powder.
Embodiment 2
The application effect of the preventing and treating in banana blight for the Streptomyces jingyangensis wettable powder of the present invention
1 test period: in June, -2016 in May, 2014
2. test site: Guangdong Province Xuwen County farm
3. testing program: test group: monthly drip irrigation once, consumption be the present invention 1 kilogram of Jingyang chain streptomycete wettable powder/
Mu, usable floor area is 22 mu；Matched group: using 10 kgs/acre of commercially available certain bacillus wettable powder (Fructus Musae is special), use
Area is 108 mu, and matched group uses bacillus wettable powder using in March, 2015, starts also to use from April, 2015
The Streptomyces jingyangensis wettable powder of the present invention；Other management are identical.
4. result of the test:
We have counted test group and matched group incidence, and result such as table 1 below, it can clearly be seen that test group makes from table 1
With the sickness rate of the Streptomyces jingyangensis wettable powder of the present invention As time goes on, sickness rate was more and more lower, to 2016 6
Month sickness rate has controlled to less than 1%；And matched group is in bacillus wettable powder (Fructus Musae is special) its sickness rate applied
Significantly do not controlled, also increasingly severe trend, lead to plantation master privately to change testing program in April, 2015
All of soil is all used the Streptomyces jingyangensis wettable powder of the present invention, just significantly controlled the state of an illness.
The prevention effect to banana blight for the table 1 Streptomyces jingyangensis wettable powder
| Sickness rate/time | In May, 2014 | In December, 2014 | In March, 2015 | In June, 2015 | In December, 2015 | In June, 2016 |
| Test group | 15.8% | 12.6% | 10.3% | 7.4% | 3.3% | 0.9% |
| Matched group | 12.4 | 14.4% | 16.6% | 15.2% | 10.1% | 5.6% |
Claims (2)
1. a kind of Streptomyces jingyangensis wettable powder is it is characterised in that described Streptomyces jingyangensis wettable powder is by following weight
Part composition: Streptomyces jingyangensis zymophyte powder 90-95 part；Wetting agent: dodecylbenzene sodium sulfonate 1-4 part；Dispersant: tea saponin
0.2-1 part；Uv-protector: ascorbic acid 0.5-2 part, powder-beta-dextrin 0.2-1 part；Drying protectant: maltose 1-4 part, manna
Alcohol 0.2-1 part；Its concrete preparation method, is made up of suddenly the following steps:
First, the preparation of Streptomyces jingyangensis zymophyte powder
Take out culture presevation pipe, draw flat board with Gause I solid medium and recovered, cultivate 4-5 days, under flat board for 30 DEG C
Picking single bacterium colony is seeded on Gause I solid medium in Fructus Solani melongenae bottle, fills whole Fructus Solani melongenae bottle, 30 DEG C in incubator
Culture 4-6 days, waits phage surface to have baby pink spore, the lawn in Fructus Solani melongenae bottle is washed down by 1l sterilized water, will wash down
Streptomyces jingyangensis be inoculated in 500l seed fermentation tank equipped with 350l Streptomyces jingyangensis fluid medium, starting ph is
7.2, temperature is 30 DEG C, and divulge information 1:1, opens stirring, and rotating speed is 200r/min；Culture 36-48 hour, treats that thalline becomes viscous, has
A large amount of mycelia produce, you can as Streptomyces jingyangensis liquid spawn；
Streptomyces jingyangensis liquid spawn is seeded in solid fermentation culture medium with 10% inoculum concentration, mixes stand to the pallet that ferments
In, the thickness of solid fermentation culture medium is 3-5cm, cultivates 2-3 days for 28-32 DEG C, treats that solid fermentation culture medium grows a large amount of whites
Bacterium point, spore content is more than 10,500,000,000 cfu/g, you can air-dries and is less than 4% to water content；
Wherein, the proportioning of described solid fermentation culture medium is: Kaolin: corn starch: bean cake powder: Rhodopseudomonas palustris liquid=
100:2:2:20；
Wherein, described Gause I culture medium: starch 20g, nacl 0.5g, kno3 1g, kh2po4 3h2o 0.5g,
Feso4 7h2o0.01g, mgso4 7h2o 0.5g, agar 18g, water 1000ml；
Wherein, described Streptomyces jingyangensis fluid medium: glucose 20 g/l, bean cake powder 20 g/l, sodium chloride 5 g/l, sulphuric acid
Magnesium 0.5 g/l, Calcium Carbonate 2 g/l, ph7.2；The condition of each medium sterilization is: 0.10-0.15mpa, and 121 DEG C sterilize 30 points
Clock；
Wherein, the preparation of Rhodopseudomonas palustris bacterium solution: take out Rhodopseudomonas palustris kind preservation pipe, use Rhodopseudomonas palustris
Solid medium is drawn flat board and is recovered, and cultivates 72 hours for 30 DEG C, intensity of illumination: 1000lex- in illumination box
6000lex, under flat board, picking single bacterium colony is inoculated in 50 milliliters of Rhodopseudomonas palustris fluid mediums, in illumination box
In 30 DEG C of Anaerobic culturel 120 hours, seed adopts 20% inoculum concentration, is seeded to the Rhodopseudomonas palustris liquid training equipped with 4l
The big triangular flask of 5l of foster base, in illumination box, 30 DEG C of Anaerobic culturel 72-96 hours, detect its bacterial concentration, and bacterial content surpasses
Cross 3,000,000,000 cfu/ml, you can as Rhodopseudomonas palustris bacterium solution；
Wherein, described Rhodopseudomonas palustris solid medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, sodium carbonate 3g, Sodium Acetate Trihydrate
1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, agar 20g, ph7.5, water 1000ml；
Wherein, described Rhodopseudomonas palustris fluid medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, sodium bicarbonate 2g, acetic acid
Sodium 4g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, trace element 10ml, ph7.5, water 1000ml；Each medium sterilization
Condition is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes；
Wherein, the preparation of trace element: manganese sulfate monohydrate 4 g, boric acid 2 g, Sodium Molybdate Dihydrate 0.05g, copper sulphate pentahydrate
0.005g, cobalt chloride hexahydrate 0.05g, water 1000ml；
2nd, by Streptomyces jingyangensis zymophyte powder 90-95 part；Wetting agent: dodecylbenzene sodium sulfonate 1-4 part；Dispersant: tea saponin
0.2-1 part；Uv-protector: ascorbic acid 0.5-2 part, powder-beta-dextrin 0.2-1 part；Drying protectant: maltose 1-4 part, manna
Alcohol 0.2-1 part；Mix and cross and 400 mesh sieves were pulverized by jet mill, detect that its Streptomyces jingyangensis spore content is not less than
10000000000 cfu/g, suspensibility is not less than 85%, and wetting time is not higher than 85s, and moisture is less than 5%, is vacuum-packed, as Jingyang
Streptomycete wettable powder.
2. a kind of Streptomyces jingyangensis wettable powder according to claim 1, its feature, are that described Streptomyces jingyangensis can
WP is grouped into by following weight: by 90 parts of Streptomyces jingyangensis zymophyte powder；Wetting agent: 3 parts of dodecylbenzene sodium sulfonate；
Dispersant: 0.7 part of tea saponin；Uv-protector: 1.5 parts of ascorbic acid, 0.6 part of powder-beta-dextrin；Drying protectant: maltose 3
Part, 0.8 part of Mannitol.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610733594.6A CN106342900B (en) | 2016-08-28 | 2016-08-28 | A kind of Streptomyces jingyangensis wettable powder and preparation method thereof |
Applications Claiming Priority (1)
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| CN116836892A (en) * | 2023-08-30 | 2023-10-03 | 中国热带农业科学院三亚研究院 | Culture method of 5406 streptomycete capable of simultaneously producing coumarin and salicylic acid |
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| CN116836892A (en) * | 2023-08-30 | 2023-10-03 | 中国热带农业科学院三亚研究院 | Culture method of 5406 streptomycete capable of simultaneously producing coumarin and salicylic acid |
| CN116836892B (en) * | 2023-08-30 | 2023-11-14 | 中国热带农业科学院三亚研究院 | Culture method of 5406 streptomycete capable of simultaneously producing coumarin and salicylic acid |
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