CN105503391A - Functional foliage fertilizer for preventing and controlling powdery mildew of tomatoes and cucumbers and application - Google Patents
Functional foliage fertilizer for preventing and controlling powdery mildew of tomatoes and cucumbers and application Download PDFInfo
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- CN105503391A CN105503391A CN201510956417.XA CN201510956417A CN105503391A CN 105503391 A CN105503391 A CN 105503391A CN 201510956417 A CN201510956417 A CN 201510956417A CN 105503391 A CN105503391 A CN 105503391A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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Abstract
The invention discloses a functional foliage fertilizer for preventing and controlling powdery mildew of tomatoes and cucumbers and application. The main raw materials for the foliar fertilizer include a solution of hydrolyzed starch Bacillus amyloliquefaciens YN2010-KC with CGMCC No.5722, other microbial bacterial solutions, a nitrogen source, a phosphorus source, a potassium source, trace element raw materials, humic acid and a stabilizer, and the functional foliage fertilizer is prepared through compounding in proportion. The foliar fertilizer is a novel ecological and safe fertilizer and can be used for foliar application and root irrigation of crops. The prevention and control effect on the powdery mildew of vegetables is high, the prevention and control effect on the powdery mildew of the tomatoes is up to 77.95-92.37%, the prevention and control effect on the powdery mildew of the cucumbers is up to 71.80-81.19%, the functional foliage fertilizer is non-toxic, residual-free, pollution-free and free of toxic and side effects, does not contain any harmful chemical substance, fertilizers can be decreased or neither fertilizer or pesticide is used, an entire ecological system is changed, and high-quality pollution-free agricultural products are produced out.
Description
Technical field
The present invention relates to a kind of agriculture production for preventing and treating the foliage fertilizer of Powdery Mildew, in particular, relating to a kind of multifunctional microbial foliage fertilizer for foliage-spray, also can be used for filling with root use, belong to technical field of fertilizers.
Background technology
Powdery mildew (Erysiphales) is the plant pathogenic fungi of specialization, belong to Ascomycotina (Ascomycotina) gang pyrenomycetes (Pyrenomycetes) Erysiphales (Erysiphales) Erysiphaceae (Erysiphaceae), mostly be ectoparasitism, whole or the large portion of mycelium is exposed to the leaf of host plant, stem, the tender tip, bud, flower and fruit real surface, and produces the macroscopic white powder thing be made up of mycelium, conidiophore and conidium in a large number.
Powdery Mildew has 18 to belong to totally 435 kinds in China, of a great variety, widely distributed, and harm is serious.After Powdery Mildew occurs, host's position growth of being injured is suppressed, moves back green flavescence gradually, occurs that withered spot is dried-up to last full leaf, if many blades are injured simultaneously, plant also can be made early withered.The disease later stage, in the mould layer of white powder shape be made up of mycelia and conidium, the cleistothecium that the much scattered black of normal appearance is granular, and survive the winter with this, the secondary spring carries out First aggression with thecaspore.The conidium of Powdery Mildew all can sprout at 10-30 DEG C, not high to humidity requirement, within 10 days, can complete an Infection cycie under normal circumstances, and farm crop Growing season is energy-conservation to be infected repeatedly, once generation will very fast its spread in china.Powdery Mildew makes the crop products such as China's wheat, cucumber, tomato, grape be subjected to extreme loss, has had a strong impact on the development of China's agricultural.Therefore, how effectively to prevent the generation of Powdery Mildew and propagate the major issue just become in agriculture field.
At present, the method for preventing and treating the Powdery Mildew such as tomato, cucumber mainly contains breeding for disease resistance, chemical prevention and biological control etc.Because breeding for disease resistance is difficult to carry out and resistance is easily lost; There is the problem easily developed immunity to drugs and to people, environmental pollution in chemical prevention, application limits by condition.In recent years, biological control method is with strong points owing to having controlling object, and preventive effect is high, and to people, animal safety, does not have the advantages such as environmental pollution and be subject to increasing attention.With the new-type fertilizer that bio-feritlizer, biological organic fertilizer and biological foliage fertilizer are representative, its development prospect is quite wide.
Summary of the invention
The object of the invention is to provide one and can strengthens mildew-resistance evil ability, alleviate white powder pathogenetic functional microorganism foliage fertilizer product.
To achieve these goals, technical scheme of the present invention is as follows:
1. a control tomato or powdery mildew of cucumber functional leaf leaven, be made up of the following raw material in massfraction: bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 bacterium liquid 10 ~ 20%, other microbial inoculum 10 ~ 20%, nitrogen source raw material 3 ~ 10%, phosphorus source raw material 1 ~ 4%, potassium source raw material 3 ~ 6%, humic acid 4 ~ 8%, trace element materials 0.5 ~ 2%, stablizer 0.1 ~ 0.5%, water is surplus, and pH value is 6.5 ~ 7.2.
2. the control tomato according to technical scheme 1 or powdery mildew of cucumber functional leaf leaven, is characterized in that:
You effect Jun Shuo≤2.0 × 10 alive of described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 bacterium liquid
7cfu/mL;
Other microbial inoculum described is photosynthetic bacteria (Photosynthetic) bacterium liquid, silicate bacteria (Bacillusmucilaginosus) bacterium liquid, any one or any two or more in vinelandii (Azotobacter) bacterium liquid, during for any two or more bacterium liquid, the weight proportion between various bacterium liquid is equivalent; You effect Jun Shuo≤2.0 × 10 alive of described photosynthetic bacteria (Photosynthetic) bacterium liquid
7cfu/mL; You effect Jun Shuo≤2.0 × 10 alive of silicate bacteria (Bacillusmucilaginosus) bacterium liquid
7cfu/mL; You effect Jun Shuo≤2.0 × 10 alive of vinelandii (Azotobacter) bacterium liquid
7cfu/mL;
Described nitrogen source raw material is: any one or any two or more in urea, ammonium nitrate, bicarbonate of ammonia, nitrogen source raw material be any two or more time, each nitrogen source raw material is equivalent;
Described phosphorus source raw material is: one or both in calcium superphosphate, potassium primary phosphate, and when phosphorus source raw material is two kinds, each phosphorus source raw material is equivalent;
Described potassium source raw material is: one or both in potassium sulfate, saltpetre, and when potassium source raw material is two kinds, each potassium source raw material is equivalent;
Described trace element materials is: the one in boric acid, ammonium molybdate, zinc sulfate, manganous sulfate, ferrous sulfate or two or more arbitrarily, trace element materials be any two or more time, each trace element materials is equivalent.
Described stablizer is: the one in xanthan gum or carboxymethyl cellulose.
3. described in technical scheme 1 or 2, prevent and treat tomato or the application of powdery mildew of cucumber functional leaf leaven in control tomato Powdery Mildew.
4. prevent and treat tomato described in technical scheme 1 or 2 or the application in powdery mildew of cucumber prevented and treated by powdery mildew of cucumber functional leaf leaven.
Compared with prior art, tool has the following advantages in the present invention:
(1) high to the prevention effect of vegetables Powdery Mildew, 77.95 ~ 92.37% are reached to the prevention effect of tomato Powdery Mildew, 71.80 ~ 81.19% are reached to the prevention effect of powdery mildew of cucumber, and nontoxic, noresidue, pollution-free, have no side effect, not containing any chemical noxious material, can to reduce or without chemical fertilizer, agricultural chemicals, change the whole ecosystem, produce high-quality non-polluted farm product expeditiously, recover the genuineness of agricultural-food, promote agricultural sustainable development.
(2) quick active plant immune system, can breed rapidly on crop, forms flora advantage, improves disease resistance ability, the special problem solving the upper Powdery Mildew of production and be difficult to prevent and treat.
(3) have accumulated a large amount of phosphoric in agricultural land soil and not absorb by farm crop, product of the present invention can stimulate the breeding of soil microorganisms, improves rhizosphere vigor, activation phosphorus and other middle and trace element, plant is better absorbed, improves the utilization ratio of fertilizer;
(4) quantity of useful microbe such as photosynthetic bacterium and vinelandii on blade can be assembled, promote crop photosynthesis.
(5) be widely used, strong adaptability.
Bacillus amyloliquefaciens BacillusamyloliquefaciensYN2010-KC of the present invention is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on January 12nd, 2012 (to be called for short: CGMCC), preserving number is CGMCCNo.5722, and is detected as survival on January 12nd, 2012.This common micro-organisms centre address: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode: 100101.
The present invention prevents and treats tomato or the various raw material of powdery mildew of cucumber functional leaf leaven.Except bacillus amyloliquefaciens BacillusamyloliquefaciensYN2010-KCCGMCCNo.5722 of the present invention is except above-mentioned China Committee for Culture Collection of Microorganisms's common micro-organisms center, all the other raw materials are commercially available.Described photosynthetic bacteria (Photosynthetic) bacterium liquid, silicate bacteria (Bacillusmucilaginosus) bacterium liquid, vinelandii (Azotobacter) bacterium liquid all can be bought from above-mentioned China Committee for Culture Collection of Microorganisms's common micro-organisms center.
Embodiment
Further illustrate below in conjunction with embodiment.Following examples are not construed as limiting the present invention, and those skilled in the art are improving technique parameter on this basis, change concrete auxiliary material type etc. and replace and change, still belong to protection scope of the present invention.Following examples are ordinary method without specified otherwise.
The acquisition of embodiment 1 bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KC, preservation
1, the acquisition of bacillus amyloliquefaciens YN2010-KC of the present invention
1.1 material
Pick up from the soil around the Rhizosphere of Crops of Yunnan Prov Agriculture University Hou Shan farm.
1.2 medium preparing
LB solid medium and PSA substratum (potato sucrose nutrient agar) are melted, pours sterilizing culture dish respectively into, cooling, make LB and PSA dull and stereotyped.
1.3 separation and Culture
Adopting dilution plate partition method to be separated with plate streaking, is YN2010-KC by a Strain Designation of separation and purification gained.
2, the qualification of bacillus amyloliquefaciens YN2010-KC
2.1, Morphological Identification
Get bacterial strain YN2010-KC to cultivate on LB substratum, cultivate 36 hours, carry out morphologic observation qualification for 37 DEG C, thalline is shaft-like, end circle, and peritrichous, tool mobility, produce oval gemma; On LB and PSA flat board, bacterium colony circle, weak tea brown, bacterium colony central color slightly dark, protuberance, surface drying has fold.(eastern elegant pearl etc. are write with " common bacteria system identification handbook ", Science Press, calendar year 2001) in describe bacillus morphological specificity basically identical, tentatively judge that bacterial strain YN2010-KC belongs to genus bacillus (Bacillusspp.).
2.2, Physiology and biochemistry qualification
Bacterial strain gramstaining, spore staining, Starch Hydrolysis, glucose utilization, Citrate trianion utilization, V-P reaction, catalase reaction, nitrate reduction, gelatine liquefication etc. are all positive.
3, the preservation of bacillus amyloliquefaciens YN2010-KC
Through above-mentioned identify show be separated obtain bacterial strain be bacillus amyloliquefaciens (Bacillusamyloliquefaciens), its code name is YN2010-KC, described bacillus amyloliquefaciens BacillusamyloliquefaciensYN2010-KC is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on January 12nd, 2012 (to be called for short: CGMCC), preserving number is CGMCCNo.5722, and is detected as survival on January 12nd, 2012.This common micro-organisms centre address: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, postcode: 100101.
Embodiment 2 the present invention prevents and treats tomato or powdery mildew of cucumber function foliage fertilizer formulation and preparation
1, the present invention prevents and treats tomato or powdery mildew of cucumber function foliage fertilizer formulation is shown in as following table 1-table 3
Table 1 prevents and treats tomato or powdery mildew of cucumber functional leaf leaven No. 1 formula for a product
Table 2 prevents and treats tomato or powdery mildew of cucumber functional leaf leaven No. 2 formula for a product
Table 3 prevents and treats tomato or powdery mildew of cucumber functional leaf leaven No. 3 formula for a product
Table 1, table 2, table 3 each control tomato or powdery mildew of cucumber functional leaf leaven are respectively referred to as No. 1 product, No. 2 products, No. 3 products.
2, the preparation method of above each product:
By formula for a product metering, taking each component, after each component fully being mixed, is 6.5 ~ 7.2 namely obtain control tomato or powdery mildew of cucumber functional leaf sourdough product by ferrous sulfate adjust ph.Described living bacteria count is 2.0 × 10
7the preparation of bacillus amyloliquefaciens (Bacillusamyloliquefaciens) the YN2010-KCCGMCCNo.5722 bacterium liquid of cfu/mL: bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 will be stated and be inoculated in LB liquid medium, under culture temperature 35 DEG C, rotating speed 120rpm/min condition, the living bacteria count that shaking table cultivates 36 ~ 48h, bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 is 2.0 × 10
7during cfu/mL, obtaining described living bacteria count is 2.0 × 10
7bacillus amyloliquefaciens (Bacillusamyloliquefaciens) the YN2010-KCCGMCCNo.5722 bacterium liquid of cfu/mL.
Embodiment 3 the present invention prevents and treats tomato or the application of powdery mildew of cucumber functional leaf leaven in control tomato Powdery Mildew
1. test period: in September, 2014-2014 year December.
2. test site: Anning City, Yunnan Province Qinglong Zhen Vegetable Base carries out.
3. for studying thing
Supplying to study thing is bright red 168 tomatoes (Powdery Mildew susceptible variety)
4. test method
Duplicate rows is cultivated, line-spacing 60cm, spacing in the rows 40cm, and adopt the group arrangement of single-factor random district, 1 community establishes 5 to be treated to 1 repetition, if 4 repetitions, and each plot area 7.2m
2.Training is gone alone in unified employing, when there is 5 fruit branches, manually binds.
No. 1 product dilutes 5 times respectively, 50 times, and 100 times of 3 concentration, are treated to blank with clear water, and 25% Azoxystrobin wettable powder 2000 times liquid is positive control, sprays when tomato leaf begins to show scab.Spray once every 7d, spray 3 times altogether, respectively with on November 12nd, 2014, November 19, December 26 sprayed, and 100 blades of each process of 7d random selecting after each dispenser carry out disease level statistics; After statistics terminates the last time, then carried out a tracking investigation every 7 days, totally 3 times.Test is treated to:
The aqueous solution of process 1, CK:0.2% xanthan gum.
Process 2, No. 1 product × 5:1 product 5 times diluent.
Process 3, No. 1 product × 50:1 product 50 times diluent.
Process 4, No. 1 product × 100:1 product 100 times diluent.
Process 5,25% Azoxystrobin wettable powder 2000 times of liquid, effective concentration 125mg/L.
5. results and analysis
In the greenhouse experiments scheduling to last 42d, product of the present invention shows higher protection effect all the time.
Table 1 embodiment 3 respectively processes tomato controlling powdery mildew effect
In 7d investigation result after first time dispenser, No. 1 product × 5 just show the preventive effect of 45.94%, higher than process 5 (chemical agent 25% Azoxystrobin wettable powder 2000 times of liquid), but the preventive effect of No. 1 product × 50 and No. 1 product × 100 is about 15%, lower than process 5.7d after second time sprays, the disease index of each process declines obviously, and wherein the preventive effect of No. 1 product × 5, No. 1 product × 50, No. 1 product × 100 reaches 83.19%, 65.48% and 62.72% respectively, find in 7d (December 3) investigation result after spraying 3 times continuously, all products containing bacillus amyloliquefaciens of the present invention (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 bacterium liquid are all in rising trend to the prevention effect of tomato Powdery Mildew, and the spraying concentration of preventive effect and bacillus amyloliquefaciens of the present invention (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 bacterium liquid presents positive correlation, wherein the preventive effect of No. 1 product × 5 is up to 92.37%, the preventive effect of No. 1 product × 50 is 77.95%, the preventive effect of No. 1 product × 100 is 71.37%, 56.75% is improved respectively than the process 5 of chemical agent 25% Azoxystrobin wettable powder, 32.28%, 21.11%, and significant difference (table 1) each other.Now, CK morbidity is to late period, disease index reaches 79.89, by infecting of Powdery Mildew germ, lower blade has started to wither and fall, and prevention effect shows as No. 1 product × 5>1 product × 50>1 product × 100> chemical agent 25% Azoxystrobin wettable powder.
The protection effect persistence investigation that table 2 embodiment 3 respectively processes
Spray after investigation terminates the last time, the tracking investigation carrying out 21d by a definite date December 3 found that: when stopping products applied, the process of once using product all keeps higher prevention effect, and preventive effect quite stable.Through process No. 1 product × 5 of product 5 times dilution in whole process to show efficient of the controllability of Powdery Mildew germ and lastingly, preventive effect remains at more than 92%; December 10, the preventive effect of No. 1 product × 50 is suitable with No. 1 product × 100 preventive effect, both remain on 70%-71% at preventive effect, the preventive effect of No. 1 product × 100 starts higher than No. 1 product × 50 after this, both preventive effects are intersected, but No. 1 product × 50, No. 1 both product × 100 preventive effect all remain between 66%-77%.As seen from Table 2, product of the present invention has good prevention effect to tomato Powdery Mildew, spray the effect that can reach preventive effect and constantly raise continuously, and preventive effect is directly proportional to spraying concentration; After stopping sprays, the plant of once using product of the present invention still has higher preventive effect, and this shows product of the present invention, and to control tomato Powdery Mildew, not only preventive effect is high, and the preventive effect extended period is long.
Embodiment 4 the present invention prevents and treats tomato or the application in powdery mildew of cucumber prevented and treated by powdery mildew of cucumber functional leaf leaven
1. test period: in December ,-2014 in September, 2014
2. test site: Anning City, Yunnan Province Qinglong Zhen Vegetable Base carries out.
3. for studying thing
For studying thing for improvement ' Tianjin grinds No. four '
4. test method
Duplicate rows is cultivated, line-spacing 60cm, spacing in the rows 40cm, and adopt the group arrangement of single-factor random district, 1 community establishes 5 to be treated to 1 repetition, if 4 repetitions, and each plot area 7.2m2.Training is gone alone in unified employing.
No. 2 products dilute 5 times respectively, 50 times, and 100 times of 3 concentration, are treated to blank with clear water, and 25% Azoxystrobin wettable powder 2000 times liquid is positive control, sprays, until blade surface evenly adheres to water membrane when cucumber leaves begins to show scab.Spray once every 7d, spray 3 times altogether, and 100 blades of each process of 7d random selecting after each dispenser carry out disease level statistics; After statistics terminates the last time, then carried out a tracking investigation every 7 days, totally 3 times.Test is treated to:
The aqueous solution of process 1, CK:0.2% xanthan gum.
Process 2, No. 2 product × 5:2 product 5 times diluents.
Process 3, No. 2 product × 50:2 product 50 times diluents.
Process 4, No. 2 product × 100:2 product 100 times diluents.
Process 5,25% Azoxystrobin wettable powder 2000 times of liquid, effective concentration 125mg/L.
5. results and analysis
The prevention effect (table 3) of the 7th day after continuous 3 dispensers of each process of different weaker concn shows: all there were significant differences with blank after 5,50,100 times of dilution process for the present invention's No. 2 products, preventive effect reaches 65.04% ~ 81.19%, wherein product of the present invention preventive effect when dilution 5 times is up to 81.19%, and product of the present invention improves 18.98%, 5.22% through 5,50 times of dilution process respectively than the preventive effect of Chemical treatment 5.
Table 3 embodiment 4 respectively processes powdery mildew of cucumber prevention effect
Claims (4)
1. a control tomato or powdery mildew of cucumber functional leaf leaven, it is characterized in that: be made up of the following raw material in massfraction: bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 bacterium liquid 10 ~ 20%, other microbial inoculum 10 ~ 20%, nitrogen source raw material 3 ~ 10%, phosphorus source raw material 1 ~ 4%, potassium source raw material 3 ~ 6%, humic acid 4 ~ 8%, trace element materials 0.5 ~ 2%, stablizer 0.1 ~ 0.5%, water is surplus, and pH value is 6.5 ~ 7.2.
2. control tomato according to claim 1 or powdery mildew of cucumber functional leaf leaven, is characterized in that:
You effect Jun Shuo≤2.0 × 10 alive of described bacillus amyloliquefaciens (Bacillusamyloliquefaciens) YN2010-KCCGMCCNo.5722 bacterium liquid
7cfu/mL;
Other microbial inoculum described is photosynthetic bacteria (Photosynthetic) bacterium liquid, silicate bacteria (: Bacillusmucilaginosus) bacterium liquid, any one or any two or more in vinelandii (Azotobacter) bacterium liquid, during for any two or more bacterium liquid, the weight proportion between various bacterium liquid is equivalent; You effect Jun Shuo≤2.0 × 10 alive of described photosynthetic bacterium (Photosynthetic) bacterium liquid
7cfu/mL; You effect Jun Shuo≤2.0 × 10 alive of silicate bacteria (Bacillusmucilaginosus) bacterium liquid
7cfu/mL; You effect Jun Shuo≤2.0 × 10 alive of vinelandii (Azotobacter) bacterium liquid
7cfu/mL;
Described nitrogen source raw material is: any one or any two or more in urea, ammonium nitrate, bicarbonate of ammonia, nitrogen source raw material be any two or more time, each nitrogen source raw material is equivalent;
Described phosphorus source raw material is: one or both in calcium superphosphate, potassium primary phosphate, and when phosphorus source raw material is two kinds, each phosphorus source raw material is equivalent;
Described potassium source raw material is: one or both in potassium sulfate, saltpetre, and when potassium source raw material is two kinds, each potassium source raw material is equivalent;
Described trace element materials is: the one in boric acid, ammonium molybdate, zinc sulfate, manganous sulfate, ferrous sulfate or two or more arbitrarily, trace element materials be any two or more time, each trace element materials is equivalent.
Described stablizer is: the one in xanthan gum or carboxymethyl cellulose.
3. described in claim 1 or 2, prevent and treat tomato or the application of powdery mildew of cucumber functional leaf leaven in control tomato Powdery Mildew.
4. prevent and treat tomato described in claim 1 or 2 or the application in powdery mildew of cucumber prevented and treated by powdery mildew of cucumber functional leaf leaven.
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| CN106397005A (en) * | 2016-08-25 | 2017-02-15 | 沈阳富碳农业科技有限公司 | Carbon-based biological foliar fertilizer, or carbon-based biological water-flush fertilizer |
| CN106701618A (en) * | 2016-12-09 | 2017-05-24 | 武汉科诺生物科技股份有限公司 | Bacillus amyloliquefaciens resistant to powdery mildew of melon crops and high in spore yield |
| CN106748259A (en) * | 2017-01-17 | 2017-05-31 | 四川鹤岛农业科技有限公司 | A kind of retain water and nutrients composite microbic bacterial fertilizer and application |
| CN108377819A (en) * | 2018-02-05 | 2018-08-10 | 云南省烟草公司普洱市公司 | A method of prevention tobacco black shank |
| CN109294947A (en) * | 2018-10-08 | 2019-02-01 | 山东蔚蓝绿源生物科技有限公司 | A kind of separation screening and zymotechnique of the bacillus amyloliquefaciens for powdery mildew |
| CN109761706A (en) * | 2019-03-21 | 2019-05-17 | 南京市蔬菜科学研究所 | A kind of foliar fertilization method for promoting vegetable increase production to increase matter |
| CN111713206A (en) * | 2020-06-19 | 2020-09-29 | 四川省农业科学院植物保护研究所 | Method for increasing yield of bitter gourds by using biocontrol strain bacillus amyloliquefaciens Bam22 |
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