KR20130005322A - Bacillus amyloliquefaciens lm11 strain, composition for control plant disease and control method of plant disease with same - Google Patents

Abstract

PURPOSE: A novel Bacillus amyloliquefaciens, a composition containing the same, and a method for preventing plant diseases are provided to biologically prevent various plant diseases. CONSTITUTION: A Bacillus amyloliquefaciens LM11(KACC91643P) prevents plant pathogens. The plant pathogens are Magnaporthe oryzae, Thanatephorus cucumeris, Botrytis cinerea, Phytophthora infestans, Puccinia recondite, Blumeria graminis f. sp. Hordei, or Colletotrichum coccodes. A composition for preventing plant diseases contains the strain or a culture thereof. The plant diseases are blast, sheath blight, gray mold, leaf rust, powdery mildew, or anthracnose. A method for preventing plant diseases comprises a step of treating the composition to crops or an environment for cultivating the crops. The dose of the strain is 1×10^5 cfu/ml to 1×10^12 cfu/ml. [Reference numerals] (AA) Control value(%)

Description

Bacillus amyloliquefaciens LM11 strain, composition for controlling plant diseases and the method for controlling plant diseases

The present invention relates to a Bacillus amyloquiquiciens LM11 strain, a composition for controlling plant diseases containing the same, and a method for controlling plant diseases.

Pesticides and fertilizers, the products of modern science, have greatly contributed to the liberation of humankind from hunger by increasing agricultural productivity. Recently, however, there has been a great deal of social attention on negative aspects such as phosphorus and livestock toxicity and environmental pollution caused by misuse and residue of pesticides and fertilizers, rather than the positive aspects of synthetic pesticides and fertilizers. With the advent of the well-being era, there is an increasing demand for eco-friendly agricultural products in which synthetic pesticides are used or crops are grown without pesticides. In addition, farmers are also importing a lot of low-priced foreign agricultural products by import liberalization, and as an alternative, organic agricultural products with a high income per unit area are preferred.

However, it is very difficult to harvest crops without controlling plant diseases when crops are grown with environmentally friendly farming methods in actual packaging. Studies show that peaches and apples lost 100% and 97%, respectively, and vegetables such as cucumbers, cabbages, and tomatoes lost between 39 and 63% when grown without crop protection. Therefore, in order to produce eco-friendly agricultural products, there is a need for biopesticides having excellent effects that can replace synthetic pesticides.

Biopesticides include microbial pesticides that control plant diseases, pests and weeds using microorganisms, and biochemical pesticides that use natural products produced by plants or microorganisms. In the case of microbial pesticides, it is difficult to mass-produce and formulate products, and the preservation stability of the products is low, and thus the market is not greatly expanded because standardization, standardization, and ease of distribution are not secured like chemical pesticides. In addition, when the microbial pesticide is used for the actual packaging, the plant disease can be controlled only after the microorganisms have grown in a significant amount after treatment, and thus there is a difficulty in controlling the plant disease in a short time. In addition, because the spectrum of the applied pests are narrow, various biopesticides have to be treated in order to control other plant diseases occurring in crops, which is a real economic burden for farmers.

On the other hand, Bacillus bacteria, which are mainly present in the root zone of the soil, produce various secondary metabolites that exhibit antimicrobial activities such as suctinine and iturin, and are Gram-positive, characterized by forming endogenous spores that are resistant to adverse environments such as heat. It is a bacterium. In addition, since most of the strains of the genus Bacillus are safe for phosphorus and axial toxicity, in recent years, many studies have been conducted to develop biological control agents using them.

In Korea, Bacillus amyloliquefactions LP03 strain (Korean Patent No. 0807403), Bacillus alopeas CNU05-1 strain (Korean Patent No. 0773091), and Bacillus for controlling plant diseases as strains for control of gray mold among microorganisms of Bacillus spp. Subtilis KCCM10639 or KCCM10640 strain (Korean Patent No. 0767437) has been registered.

A number of microbial control agents exhibiting antifungal activity as strains of the Bacillus genus have been disclosed, but biopesticides that can effectively control various plant diseases have not yet been established, and thus, development of new biological control agents is required. .

In addition, in the past, microorganisms, which mainly have antimicrobial activity against pathogens, have been used for biological control, but recently, resistance reactions induced in crops by inoculating microorganisms in advance with the purpose of inducing resistance to diseases in crops ( Induced Systemic Resistance ("ISR") is used. The latter method has been evaluated as a very desirable control method in that the control effect is long lasting in addition to the advantage that it does not cause problems such as environmental pollution, weakness and residual toxicity.

KR 10-0773091 B1 2007.10.29 KR 10-0767437 B1 2007.10.09 KR 10-0807403 B1 2008.02.19

Therefore, the inventors of the present invention, Bacillus amyloquiquisis ( Bacillus) isolated from insect feces amyloliquefaciens ) By spraying or irrigating LM11 strains on plants, they not only control various plant diseases biologically, but also induce resistance to activate immune functions on the plants themselves, and thus have excellent control effects against various plant diseases. It was confirmed that the present invention was completed.

It is an object of the present invention to provide a novel Bacillus amyloliquiphysis ( Bacillus) having excellent control against various plant pathogens. amyloliquefaciens ) to provide a LM11 strain and its culture production method.

Still another object of the present invention is to provide a plant disease control composition containing the Bacillus amyloliquefaciens LM11 strain and its culture.

Still another object of the present invention is to provide a method for controlling plant diseases using the composition for controlling plant diseases.

In order to achieve the above object, the present invention provides a Bacillus amyloliquefaciens LM11 strain (KACC91643P) having a control activity against plant pathogens.

Bacillus amyloliquefaciens LM11 strain (KACC91643P) according to the present invention is Magnaporthe oryzae , Thanatephorus cucumeris , Botrytis cinerea , pie Saratov shoot from inpe Afghanistan's (Phytophthora infestans), Puccini Oh rekon dita (Puccinia recondita), blue Almeria Grammy Nice form Spanish system Hor Day (Blumeria graminis f. sp. hordei ) and Colle sat tree Colchicum Coco death (Colletotrichum coccodes) It is characterized by having a control activity against at least one selected plant pathogen.

Bacillus amyloliquefaciens according to the present invention ( Bacillus amyloliquefaciens ) LM11 strain (KACC91643P) or a composition containing the culture thereof is rice blast disease (pathogen: Magnaporthe oryzae ), rice leaf blight (pathogen: Thanatephorus cucumeris ), wheat red Rust disease (pathogen: Puccinia recondita ), pepper anthrax (pathogen: Colletotrichum coccodes ), barley flour (pathogen: Blumeria graminis f. sp. hordei ), tomato gray mold (pathogen: Botrytis cinerea ), tomato late blight (pathogen: Phytophthora infestans ) is characterized by having a control activity against at least one plant disease selected from.

Hereinafter, the present invention will be described in detail.

The present invention provides a Bacillus amyloliquefaciens LM11 strain (KACC91643P) having control activity against plant pathogens.

More specifically, Bacillus amyloquiquisis according to the present invention ( Bacillus amyloliquefaciens ) The LM11 strain is isolated from insect feces. As a result of analyzing the sequencing of the 16S rDNA of the strain, Bacillus amylolyquipiciens ( Bacillus) amyloliquefaciens ) was confirmed. The novel strain is Bacillus amyloliquiquix ( Bacillus amyloliquefaciens ) was named LM11 and was deposited on April 22, 2011 at the National Institute of Agricultural Science, National Agricultural Science Institute, and was assigned accession number KACC91644P. See FIGS. 1 and 2.

The present invention Bacillus amyloliquiquix ( Bacillus amyloliquefaciens ) provides a culture of LM11 strain (KACC91643P) and a method of producing the same.

Amyloquiquisis of the present invention ( Bacillus amyloliquefaciens ) LM11 strain may be cultured in a common medium for bacterial growth, such as tryptic soy agar medium, nutrient agar, and Lubria-Bertani agar. In addition, while general bacteria grow well at a relatively high temperature of about 30 ° C to 40 ° C and do not grow well when the temperature is low, amyloquiquisis according to the present invention ( Bacillus) amyloliquefaciens ) The growth temperature of the LM11 strain is well cultured even at 20 to 30 ° C., considering that the onset temperature of major plant diseases is 20 to 25 ° C., it has a great advantage as a biological control agent for plant disease control.

The present invention Bacillus amyloliquiquix ( Bacillus amyloliquefaciens ) LM11 strain (KACC91643P) or the Bacillus amyloliquicisce Bacillus amyloliquefaciens) containing a culture of the strain LM11 (KACC91643P), it provides a composition for controlling plant diseases.

In addition, Bacillus amyloquiquisis according to the present invention ( Bacillus amyloliquefaciens ) The LM11 strain shows no pathogenicity against crops such as rice, tomato, wheat, barley, and red pepper, so it can be used as a biological control agent.

More specifically, Bacillus amyloliquicisce Bacillus amyloliquefaciens ) Rice blasting disease (pathogen: Magnaporthe) by spraying a plant with culture dilutions containing LM11 strain (KACC91643P) at a concentration of 1 × 10 ⁵ cfu / ml to 1 × 10 ¹² cfu / ml grisea ), rice leaf blight (pathogen: Thanatephorus cucumeris ), wheat red rust (pathogen: Puccinia recondita ), pepper anthrax (pathogen: Colletotrichum coccodes ), barley powdery mildew (pathogen: Blumeria graminis f. sp. hordei ), tomato gray mold (pathogen: Botrytis cinerea ), tomato late blight (pathogen: Phytophthora infestans ) and various plant diseases, such as rice blast, rice leaf blight blight, wheat red rust disease, pepper anthrax, barley powdery disease can be found to effectively control.

Bacillus amyloquiquisis according to the present invention ( Bacillus amyloliquefaciens ) Spread or irrigate the LM11 strain itself, its culture or a composition comprising an extract of the culture in an amount that can suppress plant diseases on the environment in which the plant is growing (eg soil, water) or on the surface of a growing plant. As a result, various plant diseases can be biologically controlled. At this time, if necessary, it can be used as a composition for controlling plant diseases by mixing with a carrier commonly used in the field of pesticides and formulated in powder, pellets, granules or solutions. The carrier may be water, white carbon, kaolin, and the like, and the culture may be a liquid culture or a solid culture.

The plant disease is any one or more selected from rice blast, rice leaf blight, tomato ash mold, tomato late blight, wheat red rust disease, barley flour disease, and pepper anthrax, and is applied to crops in a conventional manner.

When treating the plant disease control composition of the present invention on the packaging, the concentration of the strain in the composition may vary depending on the density and pathogenic environment of the pathogen of the packaging, but from 1 × 10 ⁵ cfu / ㎖ to 1 × 10 ¹² cfu / ㎖ The suspension contained in the concentration can be treated to the extent that it flows down, and exhibits an excellent control effect especially at a concentration of 1 × 10 ⁸ cfu / ml to 1 × 10 ¹⁰ cfu / ml.

In addition, the composition for controlling plant diseases according to the present invention by inducing resistance (ISR) that can activate the immune function to the plant itself by spraying or irrigation treatment, which can exert effects against various diseases simultaneously in one crop. There is an advantage.

The control composition according to the present invention by a specific method is to spray on the soil, crop leaves, stems, flowers or berries, can be used by diluting the composition in water, in order to add the antimicrobial activity of the microorganism strain of the present invention It may further include foliar spray or irrigation spray. In addition, during the spraying season, the preventive treatment before plant pathogens can increase and increase the control effect.

The novel Bacillus amyloliquefaciens LM11 strain (KACC91643P) according to the present invention can not only biologically control various plant diseases by spraying or irrigation on plants, but also activate immune functions on the plants themselves. Induced resistance (ISR) is induced, can be applied to multiple crops at the same time, there is an advantage that can be effective at the same time for multiple diseases in one crop.

The novel Bacillus amyloliquefaciens LM11 strain (KACC91643P) according to the present invention has a high economical replacement effect and high performance by using microorganisms, and has an effect of increasing the commercialization and productivity of crops. have.

1 shows a nucleotide sequence of a novel Bacillus amyloliquefaciens LM11 strain (KACC91643P) 16S rDNA according to the present invention.
Figure 2 is a result confirming the phylogenetic position through 16S rDNA of the novel Bacillus amyloliquefaciens LM11 strain (KACC91643P) according to the present invention,
3 is a result confirming the control effect against plant pathogens using a novel Bacillus amyloliquefaciens LM11 strain (KACC91643P) according to the present invention,
(RCB: Rice Blast, RSB: Rice Leaf Blight, TGM: Tomato Blight, TLB: Tomato Blight, WLR: Wheat Red Rust, BPM: Barley Powder, PAN: Chilli Anthrax)
Figure 4 is a result confirming the induced resistance (induced resistance) for the novel Bacillus amyloliquefaciens LM11 strain (KACC91643P) according to the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the present invention is not limited by the following examples, and various modifications or changes can be made within the spirit and scope of the present invention to those skilled in the art.

Hereinafter, the technical and scientific terms used herein will be understood by those skilled in the art without departing from the scope of the present invention. Descriptions of known functions and configurations that may be unnecessarily blurred are omitted.

Example 1 Isolation and Identification of Bacillus Amyloquiquicision LM11

(1) Molecular systematic analysis of strain

Strains excellent in antimicrobial activity were selected from microorganisms isolated from insect feces.

For molecular analysis of the selected strains, first, 16S rDNA sequencing was performed. As a result, the nucleotide sequence of FIG. 1 (SEQ ID NO: 1) was confirmed, and the homology search was performed with the sequence database of NCBI GeneBank, and the systematic location was identified using the CLUSTAL X program and the PHYLIP program.

Molecular phylogenetic analysis based on the 16S rDNA sequence of the selected strains can be seen in Figure 2, Bacillus amyloliquefaciens (NBRC 15535T) as a strain belonging to the phylogenetic group including species of the genus Bacillus ) And a 60% flexibility relationship.

The isolated and selected strain was named Bacillus amyloliquefaciens LM11, and was deposited on April 22, 2011 at the National Institute of Agricultural Science, National Agricultural Science Institute, and was assigned accession number KACC91643P.

Example 2 Control Effect of Bacillus Amyloquiquiciens LM11 on Plant Disease

The plant diseases (plant pathogens) of Table 1 were assayed for assaying control effects against various plant diseases using the culture of the selected Bacillus amyloliquefaciens LM11 strain.

In order to prepare the crops for the experiment, put a soil top for rice and a garden top for tomatoes, cucumbers, peppers, wheat and barley in a disposable plastic pot with a diameter of 4.5 cm. Varieties: Seeds of Seogwang Tomato, Heung-Nong Seedling), Red Pepper (Bujiang, Heung-Nong Seedling), Wheat (Breed: Joung-Gwang), and Barley (Great: East Barley) are sown in each pot, and then in greenhouse (25 ± 5 ℃). Three to four leafy rice, two to three leafy tomatoes, three to four leafy peppers, one leafy wheat and barley were prepared for the experiment.

The selected Bacillus amyloliquefaciens LM11 strain was used as TSB (Tryptic soybean broth) liquid medium at 28 ° C., 200 rpm, 48 hours, or TSA (Tryptic soybean agar: Tryptic soybean). agar), and the strain obtained after the culture was used at a concentration of 0.8 to 1.5 at 600 nm OD 600 sterile water in sterile water using a UV-Visible Spectrophotometer (Shimadza, Japan).

A three-fold dilution of the culture solution of the obtained amyloliquequicis ( Bacillus amyloliquefaciens ) LM11 strain was treated by foliar spraying on rice, tomato, pepper, wheat and barley prepared before, and one day before inoculation of the plant pathogen of Table 1 above. After 7 days of treatment, the control effect on various plant diseases was calculated as the control value (%) according to Equation 1 below.

(1) Test for Rice Blast (RCB)

Magnaporthe grisea (Hebert) Barr strain (obtained from Korea Research Institute of Chemical Technology) was inoculated into rice bran agar (20 g of rice bran, 10 g of dextrose, 15 g of agar, and 1 L of distilled water). Incubated for 2 weeks in the incubator. Pathogen-grown media was rubbed with a Rubber Polishman to remove the media hyphae, and spores were formed for 48 hours on a fluorescent lighted shelf (25 ± 2 ° C.). The formed conidia were sprayed sufficiently to flow into the plant after making a concentration of spore suspension (5 × 10 ⁸ spores / ml) with sterilized distilled water. Inoculated rice was treated in a dark state for 24 hours (relative humidity 100%, 25 ± 5 ℃), and then placed for 5 days in the growth temperature of 25 ± 5 ℃, relative humidity of 70-80% and then examined the lesion area ratio.

(2) Test for Rice Sheath Blight (RSB)

After the appropriate amount of bran in a 1 L culture bottle and sterilized, Thanatephorus cucumeris strain (obtained from: Korea Research Institute of Chemical Technology) was inoculated and incubated for 7 days in a 25 ℃ incubator. The cultured mycelium masses were finely ground and inoculated evenly in a pot where rice was grown, and after 1 day of incubation in a humidified state (25 ° C), they were placed in a constant temperature / humidity chamber having a relative humidity of 80% or higher for 4 days to investigate disease occurrence. The incidence survey was to investigate the proportion of diseased area to seedling leaf area to the area of leaf area.

(3) Test for tomato gray mold (TGM)

Botrytis cinerea strain (obtained from Korea Research Institute of Chemical Technology) was inoculated into potato agar medium and incubated for 5 days in a 20 ℃ thermostat (cancer state). Spores were formed by incubating for another 7 days while crossing. Spores formed on the medium were harvested with potato dextrose broth medium (composition: 4 g of potato starch per 1 L, dextrose 20 g; purchased from Becton & Dickinson) and the spore concentration was 5 × 10 using a hemocytometer. Adjusted to ⁵ spores / ml and sprayed tomato seedlings. Inoculated seedlings were induced for 3 days in a 20 ° C wetland (95% relative humidity), and the lesion area ratio was examined.

(4) Test for Tomato Late Blight (TLB)

Pathogen Phytophthora infestans strain (obtained from Korea Research Institute of Chemical Technology) was put into oatmeal agar medium (composition: 60 g of oatmeal per 1 L, 12.5 g of agar; purchased by Beckton & Dickinson) Inoculated and incubated at 20 ° C. incubator. The spermatozoa formed in the culture medium was harvested by adding sterile distilled water, and the spores concentration was examined by using a hemocytometer under an optical microscope to produce 1 × 10 ⁵ spores / ml spore suspension, which was then subjected to low temperature treatment, and then sprayed with tomato seedlings. . The tomato seedlings inoculated with the pathogen were incubated for 1 day in a 20 ° C. chamber, and placed in a constant temperature and humidity chamber (70% relative humidity) for 2 days.

(5) Test for Wheat Leaf Rust (WLR)

The pathogen, Puccinia recondita Rob ex Desm (obtained from Korea Research Institute of Chemical Technology), is a live parasite and used as a seeding inoculation of wheat seedlings while passing directly to plants in the laboratory. Spore suspension (spore 0.67 g / L) was prepared with harvested spores and then sprayed with wheat seedlings. Seedlings were incubated for 1 day in a 20 ° C wet room and then transferred to a 20 ° C constant temperature and humidity room with a relative humidity of 70% to induce the onset, and the lesion area ratio was examined 7 days after inoculation.

(6) Test for Barley Powdery Mildew (BPM)

The pathogen Blumeria Graminis form spesis holday graminis DC f. sp. hordei Marchal) (Acquired from Korea Research Institute of Chemical Technology) is a live parasite, and was used as an inoculator for powdery mildew spores formed by artificially inoculating barley seedlings in a laboratory. Inoculated barley seedlings were placed in a constant temperature and humidity room at 20-23 ° C and a relative humidity of 70% for 7 days, and the lesion area ratio was investigated.

(7) pepper anthrax ( Red Pepper Anthracnose , RPA For

Colletotrichum , the pathogen spores formed by inoculating coccodes strain (obtained from Korea Research Institute of Chemical Technology) into oatmeal agar medium and harvested with sterile distilled water, and spore suspension was measured by blood cell under an optical microscope to make 3 × 10 ⁵ spores / ml spore suspension. Seedlings were sprayed. Pepper seedlings inoculated with pathogens were treated for 2 days in a 25 ° C wet room, and placed in a constant temperature and humidity room (70% relative humidity) for 1 to 2 days.

The control effect of Bacillus amyloliquefaciens LM11 strain for each plant disease is shown in Table 2 below.

As can be seen in Table 2 and Figure 3, Bacillus amyloliquefaciens LM11 strain showed an excellent control effect more than 80% against rice blast, rice leaf blight, wheat rust, barley powdery mildew and It was confirmed that the pepper anthrax disease exhibited a control effect of 70% or more. On the other hand, tomato gray mold and tomato late blight was found to be less than 40% of the control effect.

Therefore, the Bacillus amyloliquefaciens LM11 strain was able to effectively control rice blast, rice leaf blight, wheat red rust, wheat flour and pepper anthrax.

Example 3 Induction Resistance Assay of Bacillus Amyloquiquicision LM11

To induce resistance in plants, the induction of disease resistance was tested by artificially inoculating peppers with Phytophthora capsici , which causes pepper blight .

Pepper seeds were planted in 9cm × 8cm pots containing topsoil 10 days after sowing and when 3 ~ 4 sheets of main leaves appeared, Bacillus amyloquiquisis ( Bacillus) amyloliquefaciens ) After diluting the suspension of LM11 strain to 1 × 10 ⁸ to 10 ⁹ cfu / ml, it was irrigated about 5 ml at the root of red pepper. Five days later, Phytophthora was incubated for two days in a PDA (Petato dextrose agar). capsici ) The pathogens were diluted 1 × 10 ⁸ to 10 ⁹ cfu / ml with sterile water containing 0.85% NaCl and 0.02% siloxane, and then inoculated with the pathogen by spraying about 2ml per plant. Plants inoculated with the pathogen were left in a greenhouse (about 25 ° C.) for 10 days and then examined for survival rate (%) of the leaves.

As a result, as shown in Figure 4, Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) LM11 strain in accordance with the present invention as a result of testing the resistance inducing resistance to the late blight of pepper strains can be confirmed that the control effect is excellent.

Agricultural Biotechnology Research Institute KACC91643 20110422

<110> Jeonnam Bioindustry Foundation <120> Bacillus amyloliquefaciens LM11 strain, composition for control          plant disease and control method of plant disease with same <160> 1 <170> Kopatentin 1.71 <210> 1 <211> 1511 <212> DNA <213> Bacillus amyloliquefaciens <220> <221> gene (222) (1) .. (1511) <223> 16S rDNA <400> 1 gagtttgatc ctggctcagg acgaacgctg gcggcgtgcc taatacatgc aagtcgagcg 60 gacagatggg agcttgctcc ctgatgttag cggcggacgg gtgagtaaca cgtgggtaac 120 ctgcctgtaa gactgggata actccgggaa accggggcta ataccggatg gttgtctgaa 180 ccgcatggtt cagacataaa aggtggcttc ggctaccact tacagatgga cccgcggcgc 240 attagctagt tggtgaggta acggctcacc aaggcgacga tgcgtagccg acctgagagg 300 gtgatcggcc acactgggac tgagacacgg cccagactcc tacgggaggc agcagtaggg 360 aatcttccgc aatggacgaa agtctgacgg agcaacgccg cgtgagtgat gaaggttttc 420 ggatcgtaaa gctctgttgt tagggaagaa caagtgccgt tcaaataggg cggcaccttg 480 acggtaccta accagaaagc cacggctaac tacgtgccag cagccgcggt aatacgtagg 540 tggcaagcgt tgtccggaat tattgggcgt aaagggctcg caggcggttt cttaagtctg 600 atgtgaaagc ccccggctca accggggagg gtcattggaa actggggaac ttgagtgcag 660 aagaggagag tggaattcca cgtgtagcgg tgaaatgcgt agagatgtgg aggaacacca 720 gtggcgaagg cgactctctg gtctgtaact gacgctgagg agcgaaagcg tggggagcga 780 acaggattag ataccctggt agtccacgcc gtaaacgatg ggtgctaagt gttagggggt 840 ttccgcccct tagtgctgca gctaacgcat taagcactcc gcctggggag tacggtcgca 900 agactgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat 960 tcgaagcaac gcgaagaacc ttaccaggtc ttgacatcct ctgacaatcc tagagatagg 1020 acgtcccctt cgggggcaga gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga 1080 gatgttgggt taagtcccgc aacgagcgca acccttgatc ttagttgcca gcattcagtt 1140 gggcactcta aggtgactgc cggtgacaaa ccggaggaag gtggggatga cgtcaaatca 1200 tcatgcccct tatgacctgg gctacacacg tgctacaatg gacagaacaa agggcagcga 1260 aaccgcgagg ttaagccaat cccacaaatc tgttctcagt tcggatcgca gtctgcaact 1320 cgactgcgtg aagctggaat cgctagtaat cgcggatcag catgccgcgg tgaatacgtt 1380 cccgggcctt gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg 1440 aggtaacctt tatggagcca gccgccgaag gtgggacaga tgattggggt gaagtcgtaa 1500 caaggtagcc g 1511

Claims (9)

Bacillus amyloliquiphysis ( Bacillus) having control activity against plant pathogens amyloliquefaciens ) LM11 strain (KACC91643P). The method of claim 1,
The plant pathogen is Magnaporthe oryzae ), Thanatephorus cucumeris ), Botrytis cinerea ( botrytis) cinerea , Phytophthora infestans ), Puccinia recondita recondita , Blumeria Graminis Foam Spesses Holiday graminis f. sp. hordei ) and Colletotrichum coccodes ( Bacillus amyloquiquisis ), characterized in that at least one member selected from amyloliquefaciens ) LM11 strain (KACC91643P). Method for producing a culture of Bacillus amyloliquefaciens LM11 strain (KACC91643P) according to claim 1. Bacillus amyloquiquisis according to claim 3 ( Bacillus amyloliquefaciens ) Culture of LM11 strain (KACC91643P). The Bacillus amyl quinone Lowry fish Lyons (Bacillus according to claim 1 amyloliquefaciens) strain LM11 (KACC91643P) or 4 Bacillus amyl quinone Lowry fish Lyons (Bacillus amyloliquefaciens), plant disease controlling composition containing a culture of the strain LM11 (KACC91643P) according to. 6. The method of claim 5,
The causative agent of the plant disease is Magnaporthe oryzae ), Thanatephorus cucumeris ), Botrytis cinerea ( botrytis) cinerea , Phytophthora infestans ), Puccinia recondita recondita , Blumeria Graminis Foam Spesses Holiday graminis f. sp. hordei ) and Colletotrichum coccodes ( colletotrichum coccodes ) is a composition for controlling plant diseases, characterized in that at least one selected from. The method according to claim 6,
The plant disease is a plant disease control composition, characterized in that at least one selected from rice blast, rice leaf blight blight, tomato gray mold, tomato blight, wheat red rust, barley flour, and pepper anthrax. A method for controlling plant diseases, comprising treating the control composition according to any one of claims 5 to 7 to a crop or an environment in which the crop is growing. The method of claim 8,
The control method is Bacillus amylolyquipiance ( Bacillus amyloliquefaciens ) Plant disease control method characterized in that the LM11 strain (KACC91643P) is treated in an amount of 1 × 10 ⁵ cfu / ㎖ to 1 × 10 ¹² cfu / ㎖.

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