CN109022310B - Bacillus Bam22 for improving resistance and growth of ryegrass and use method thereof - Google Patents
Bacillus Bam22 for improving resistance and growth of ryegrass and use method thereof Download PDFInfo
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- CN109022310B CN109022310B CN201810844056.3A CN201810844056A CN109022310B CN 109022310 B CN109022310 B CN 109022310B CN 201810844056 A CN201810844056 A CN 201810844056A CN 109022310 B CN109022310 B CN 109022310B
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- 241000209082 Lolium Species 0.000 title claims abstract description 60
- 238000000034 method Methods 0.000 title claims abstract description 15
- 241000193830 Bacillus <bacterium> Species 0.000 title abstract description 26
- 230000001580 bacterial effect Effects 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 33
- 241000196324 Embryophyta Species 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000003973 irrigation Methods 0.000 claims abstract description 8
- 230000002262 irrigation Effects 0.000 claims abstract description 8
- 239000008223 sterile water Substances 0.000 claims abstract description 8
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims abstract description 6
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 7
- 238000004321 preservation Methods 0.000 claims description 7
- 208000035240 Disease Resistance Diseases 0.000 claims 1
- 238000009629 microbiological culture Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 11
- 238000007865 diluting Methods 0.000 abstract 1
- 244000025254 Cannabis sativa Species 0.000 description 5
- 241000209504 Poaceae Species 0.000 description 4
- 239000013043 chemical agent Substances 0.000 description 4
- 240000004296 Lolium perenne Species 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000272517 Anseriformes Species 0.000 description 1
- 241000289763 Dasygaster padockina Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 231100000783 metal toxicity Toxicity 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003533 narcotic effect Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- NFACJZMKEDPNKN-UHFFFAOYSA-N trichlorfon Chemical compound COP(=O)(OC)C(O)C(Cl)(Cl)Cl NFACJZMKEDPNKN-UHFFFAOYSA-N 0.000 description 1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention relates to the research field of ryegrass resistance, in particular to bacillus Bam22 for improving ryegrass resistance and growth and a using method thereof, wherein the method comprises the following steps: 1) taking a bacterial liquid of bacillus Bam22 cultured for 48h, wherein the initial bacterial concentration of the bacterial liquid is 4-6 multiplied by 109cfu/mL, diluting by using sterile water by 120 times, 2) performing root irrigation treatment on the ryegrass by using the bacterial liquid obtained in the step 1) after the ryegrass emerges, wherein the using amount of the bacterial liquid is 50-60 mL/plant, irrigating for the first time on the 6 th-8 th day after the ryegrass emerges, and irrigating for the second time on the 13 th-15 th day; the method can improve the resistance of the ryegrass to diseases, particularly to gray mold and rust disease, and obviously improve the yield of the ryegrass by increasing the early tillering number of the ryegrass.
Description
Technical Field
The invention relates to the research field of ryegrass resistance, in particular to bacillus Bam22 for improving ryegrass resistance and growth and a using method thereof.
Background
Ryegrass, plants of the gramineae, ryegrass, about 10 species, including forage and grassland grasses in temperate regions of the continental europe and some toxic weeds. Ryegrass is an important cultivated pasture and green manure crop. There are about 10 kinds of this genus, and there are 7 kinds in China. Among them perennial ryegrass and floret ryegrass are cultivated pastures with economic value. New Zealand, Australia, the United states and the United kingdom are now widely cultivated as forage for cattle and sheep. Good pasture is generally introduced and cultivated in various places. It is common in meadow meadows and roadside wetlands. Widely distributed in the area of kashmir, pakistan, europe, the warm temperate zone of asia, north africa. The rye grass is about 0.3-1 m high, and the leaves are tough and dark green. The small spikes are long on the zigzag shaped flower axis. Perennial ryegrass and italian ryegrass germinate early and are important components in grass seeds harvested in pastures and grasslands. Poison wheat is often a virulent fungal infestation, the seeds of which also contain narcotic toxic components, both of which are very dangerous for grassland animals. The ryegrass belongs to the ryegrass of the Gramineae family, grows and grows luxuriantly in spring and autumn, has tender and succulent grass quality and good palatability, and is a good feed for cattle, sheep, rabbits, pigs, chickens, geese and fishes. The grass supplying period is 10 months to 5 months in the next year, and the grass can not grow in summer. Ryegrass is a perennial sparse grass of the genus rye of the family gramineae.
Ryegrass is low in yield and is more damaged by diseases and insect pests, the cutworm and Scarabaeiform grub and other harmful pastures are mainly used after seedling emergence, related medicines such as dipterex and Baishude are generally adopted for spray prevention before dark, main diseases in the growth period such as gray mold and rust disease are also prevented and controlled by chemical agents, but the chemical agents are used for preventing and controlling symptoms and root causes on one hand, the disease problem cannot be fundamentally solved, the misfeeding of nomadic livestock can be caused, and the chemical agents are not beneficial to ecological environment protection on the other hand.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method which has good disease prevention effect on ryegrass diseases and can obviously increase the yield of the ryegrass diseases.
The invention provides a bacillus Bam22 for improving resistance and growth of ryegrass, wherein the bacillus Bam22 is preserved in the general microbiological center of China Committee for culture Collection of microorganisms (CGMCC) at 2015, 9 and 6, with the preservation number of CGMCC No. 11329.
The research of the inventor finds that the bacillus amyloliquefaciens Bam22 can promote the growth of ryegrass plants, improve the nutrient absorption condition of the plants and enhance the resistance of the ryegrass plants to adverse stress such as drought, salt damage, heavy metal toxicity and phytotoxicity, and the like, and has the characteristics of vigorous rooting, tillering capability, rich metabolites and the like, so that the resistance of the ryegrass is enhanced and the yield of the ryegrass is increased.
The invention also provides a use method of the bacillus Bam22 for improving resistance and growth of ryegrass, which comprises the following steps:
1) taking a bacterial liquid of bacillus Bam22 cultured for 48h, wherein the initial bacterial concentration of the bacterial liquid is 4-6 multiplied by 109cfu/mL, diluted by 120 times with sterile water;
2) and (3) after seedlings of the ryegrass emerge, carrying out root irrigation on the ryegrass by using the bacterial liquid obtained in the step 1), wherein the using amount of the bacterial liquid is 50-60mL per plant, irrigating for the first time on the 6 th-8 th day after seedlings emerge, and irrigating for the second time on the 13 th-15 th day.
The invention has the beneficial effects that:
1. increasing the resistance of ryegrass to diseases, in particular to gray mold and rust;
2. the yield of the ryegrass is obviously improved by increasing the early tillering number of the ryegrass;
3. the invention adopts a pure natural method, does not use chemical agents, is simple and practical, and is environment-friendly.
Detailed Description
The present invention is described in detail below by way of examples, and it should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention.
Example 1
A method for using Bacillus Bam22 for improving resistance and growth of ryegrass, comprising the following steps:
1) taking a bacterial liquid of bacillus Bam22 cultured for 48h, wherein the initial bacterial concentration of the bacterial liquid is 4 multiplied by 109cfu/mL, diluted 100-fold with sterile water;
2) after the ryegrass seedlings emerge, carrying out root irrigation on the ryegrass seedlings by using the bacterial liquid obtained in the step 1), wherein the using amount of the bacterial liquid is 50 mL/plant, irrigating for the first time on the 7 th day after emergence of the seedlings, and irrigating for the second time on the 14 th day.
The bacillus Bam22 has a preservation number of: CGMCC NO. 11329.
Example 2
A method for using Bacillus Bam22 for improving resistance and growth of ryegrass, comprising the following steps:
1) taking a bacterial liquid of bacillus Bam22 cultured for 48h, wherein the initial bacterial concentration of the bacterial liquid is 5 multiplied by 109cfu/mL, diluted 100-fold with sterile water;
2) after the ryegrass seedlings emerge, carrying out root irrigation on the ryegrass seedlings by using the bacterial liquid obtained in the step 1), wherein the using amount of the bacterial liquid is 75 mL/plant, irrigating for the first time on the 6 th day after emergence of the seedlings, and irrigating for the second time on the 13 th day.
The bacillus Bam22 has a preservation number of: CGMCC NO. 11329.
Example 3
A method for using Bacillus Bam22 for improving resistance and growth of ryegrass, comprising the following steps:
1) taking a bacterial liquid of bacillus Bam22 cultured for 48h, wherein the initial bacterial concentration of the bacterial liquid is 6 multiplied by 109cfu/mL, diluted 100-fold with sterile water;
2) after the ryegrass seedlings emerge, carrying out root irrigation on the ryegrass seedlings by using the bacterial liquid obtained in the step 1), wherein the using amount of the bacterial liquid is 100 mL/plant, irrigating for the first time on 8 days after emergence of the seedlings, and irrigating for the second time on 15 days.
The bacillus Bam22 has a preservation number of: CGMCC NO. 11329.
Example 4
A method for using Bacillus Bam22 for improving resistance and growth of ryegrass, comprising the following steps:
1) taking a bacterial liquid of bacillus Bam22 cultured for 48h, wherein the initial bacterial concentration of the bacterial liquid is 5 multiplied by 109cfu/mL, diluted 120-fold with sterile water;
2) after the ryegrass seedlings emerge, carrying out root irrigation on the ryegrass seedlings by using the bacterial liquid obtained in the step 1), wherein the using amount of the bacterial liquid is 50 mL/plant, irrigating for the first time on 8 days after emergence of the seedlings, and irrigating for the second time on 13 days.
The bacillus Bam22 has a preservation number of: CGMCC NO. 11329.
Example 5
A method for using Bacillus Bam22 for improving resistance and growth of ryegrass, comprising the following steps:
1) taking a bacterial liquid of bacillus Bam22 cultured for 48h, wherein the initial bacterial concentration of the bacterial liquid is 5 multiplied by 109cfu/mL, diluted 120-fold with sterile water;
2) after the ryegrass seedlings emerge, carrying out root irrigation on the ryegrass seedlings by using the bacterial liquid obtained in the step 1), wherein the using amount of the bacterial liquid is 100 mL/plant, irrigating for the first time on the 6 th day after emergence of the seedlings, and irrigating for the second time on the 15 th day.
The bacillus Bam22 has a preservation number of: CGMCC NO. 11329.
After the ryegrass is mature, respectively carrying out statistics on the incidence rate of gray mold and rust disease of the ryegrass irrigated by the bacillus Bam22 in the examples 1-5 and the acre yield of the ryegrass mown cut for 4 times; rye grass watered with clear water (without bacillus Bam22 treatment) was used as a control (control group) and the statistical results are shown in table 1:
(Table 1)
As can be seen from Table 1, the incidence of gray mold of ryegrass watered with the Bacillus amyloliquefaciens Bam22 bacterial liquid is reduced by 69%, the incidence of rust disease is reduced by 53.8%, and the yield per mu is increased by 14.8%.
In addition, the statistics of 25 days after sowing show that the average division number of the ryegrass in the control group is 1.2, and the tillering number of the ryegrass in the treatment group is 3.1-3.6, which indicates that the bacillus amyloliquefaciens Bam22 strain can obviously increase the early tillering number of the ryegrass.
Claims (1)
1. Bacillus amyloliquefaciens (A)Bacillus amyloliquefaciens) The application method of the strain Bam22 for improving the gray mold resistance, rust disease resistance and growth of ryegrass comprises the following steps:
1) taking a bacterial liquid of 48h cultured Bacillus amyloliquefaciens Bam22, wherein the initial bacterial concentration of the bacterial liquid is 4-6 multiplied by 109cfu/mL, diluted by 120 times with sterile water;
2) performing root irrigation on ryegrass by using the bacterial liquid obtained in the step 1) after seedling emergence of the ryegrass, wherein the using amount of the bacterial liquid is 50-60 mL/plant, irrigating for the first time on the 6 th-8 th day after seedling emergence, and irrigating for the second time on the 13 th-15 th day;
wherein, the bacillus amyloliquefaciens Bam22 is preserved in the China general microbiological culture Collection center on 9-6 th month in 2015 with the preservation number of CGMCC No. 11329.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810844056.3A CN109022310B (en) | 2018-07-27 | 2018-07-27 | Bacillus Bam22 for improving resistance and growth of ryegrass and use method thereof |
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| CN201810844056.3A CN109022310B (en) | 2018-07-27 | 2018-07-27 | Bacillus Bam22 for improving resistance and growth of ryegrass and use method thereof |
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| Publication Number | Publication Date |
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| CN109022310A CN109022310A (en) | 2018-12-18 |
| CN109022310B true CN109022310B (en) | 2022-03-22 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130005322A (en) * | 2011-07-06 | 2013-01-16 | 재단법인 전라남도생물산업진흥재단 | Bacillus amyloliquefaciens lm11 strain, composition for control plant disease and control method of plant disease with same |
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Effective date of registration: 20240530 Address after: No. 99-1, West Section of Hupan Road, Xinglong Street, Tianfu New Area, China (Sichuan) Pilot Free Trade Zone, Chengdu City, Sichuan Province, 610200, with OL-202403030 attached Patentee after: Chengdu Dizhiyuan Plant Protection Technology Co.,Ltd. Country or region after: China Address before: No. 20, jingjusi Road, Chengdu, Sichuan 610066 Patentee before: INSTITUTE OF PLANT PROTECTION, SICHUAN ACADEMY OF AGRICULTURAL SCIENCES Country or region before: China |
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