CN105483054A - Bacillus amyloliquefaciens WS-8 and application thereof - Google Patents
Bacillus amyloliquefaciens WS-8 and application thereof Download PDFInfo
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- CN105483054A CN105483054A CN201610000768.8A CN201610000768A CN105483054A CN 105483054 A CN105483054 A CN 105483054A CN 201610000768 A CN201610000768 A CN 201610000768A CN 105483054 A CN105483054 A CN 105483054A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention relates to a bacillus amyloliquefaciens subspecies WS-8 and application thereof and discloses a bacillus amyloliquefaciens subspecies. The bacillus amyloliquefaciens WS-8 subspecies is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms, the serial number is CGMCC No.11787, and the preservation date is December 4th, 2015. The invention further discloses the application of the bacillus amyloliquefaciens subspecies in preventing and treating crop blade surface airborne fungal diseases and soil-borne fungal diseases. Fermentation broth is diluted by 50 folds and is sprayed once every 7-15 days, the prevention effect on tomato leaf mold can reach 72%, and good preventing and treating effects on other airborne and soil-borne fungal diseases are also achieved; meanwhile, the strain can improve the quality of crops, effectively improve the leaf color of plants, increase the content of chlorophyll, improve the disease resistance of the plants, reduce pesticide residues and improve quality. The strain is capable of being conveniently cultured manually and high in breeding speed, facilitates mass production and has the potential to be developed into biopesticide applied in the planting process and posthavesting of vegetables for disease prevention and treatment.
Description
Technical field
The present invention relates to bacillus amyloliquefaciens WS-8 and an application thereof, belong to agriculture microorganism and biological technical field, there is the effect improved leaf look, improve chlorophyll content in leaf blades, improve plant systemic disease resistance, prevent and treat crop blade face gas borne fungus diseases, improve quality.
Background technology
The pathogenic bacteria of leaf mold is
fulviafulva(Cooke) Cif., belong to Deuteromycotina Fulvia fulva and belong to, different name is yellow cladosporium
cladosporiumfulvumCooke, be disease common in greenhouse tomato cultivation, the propagation of this disease is fast, harm is heavy, generally causes underproduction 20-30%, can reach more than 50% time serious.At present the main chemical agent that adopts is prevented and treated, but due to its Differentiation of Pathogenicity numerous and diverse and complicated, there is many physiological strains; Cause the prevention effect of chemical pesticide worse and worse, the consumption of medicament is increasing, and cause the pesticide residue of vegetables to exceed standard, planting environment is heavily polluted, and hinders the Sustainable development of agricultural.Biological pesticide relies on the advantage of its low residue, environmentally safe, and has important effect to the Sustainable development of agricultural, the protection of agroecological environment, the guarantee of food safety, and is subject to paying close attention to more and more widely and great kindness.
Genus bacillus
bacillusspp.a class aerobic or amphimicrobian, Gram-positive, sporiferous rod-shaped bacterium.The gemma that genus bacillus produces, there is the ability of the ambient pressure environment such as stronger opposing drying, heat and ultraviolet radiation, there are many bacterial strains with specific function in genus bacillus population simultaneously, many kinds of active substances can be produced, as polypeptide, cyclic peptide, amino acid, proteolytic enzyme and tonka bean camphor etc., so have a wide range of applications in the fields such as industry, agricultural, medical science and food-processing.Because Biocontrol Bacillus has the ability suppressing various plants pathogenic bacteria, and be the non-pathogenic bacteria that occurring in nature extensively exists, to person poultry harmless, environmentally friendly, therefore, enjoy the favor of domestic and international plant protection and environment protection expert.At present, Biocontrol Bacillus is widely used in the control of plant root, limb, blade and postharvest disease of fruits and vegetables.
The U.S. has 3 bacillus subtilis
bacillussubtiliswith 1 bacillus amyloliquefaciens
b.amyloliquefaciensobtain Environmental Protection Agency (EPA) merchandized handling application license, enter large scale application.Wherein the bacillus amyloliquefaciens FZB42 sterilant commodity made mixed with subtilis GB122 are called BioYield, researched and developed by Gustafson company, be applied to the disease such as Powdery Mildew, oidium, epidemic disease, gray mold of control vegetables, cherry, grape, cucurbit and English walnut.Domestic sterilant, by the end of in December, 2014, only has the wettable powder of a kind of bacillus amyloliquefaciens to register, for preventing and treating rice blast.
It is predicted, between 2014-2020 global pesticide biological pesticide on consumption and market value demand by the quick growth of acquisition more than 10%, and the Asian-Pacific area be expected to obtain increase the most fast.Along with the formulation that " 13 " plan, country more payes attention to the development of green agriculture, more focuses on the protection of ecotope, and the biological pesticide usage ratio of growing vegetables can be increasing, and the development for biological pesticide provides more wide space.Authorized and disclosed patent at present, mostly be bacillus amyloliquefaciens control silborne fungal diseases, control fruit postharvest diseases, the correlative study of control vegetables blade face fungal disease is less, utilizes bacillus amyloliquefaciens to prevent and treat leaf muld of tomato and has no relevant report.
Summary of the invention
The object of this invention is to provide a kind of bacillus amyloliquefaciens and uses thereof, this bacterial strain is suitable for open country and canopy room condition, can improve plant systemic disease resistance, can control Plant Leaf face gas borne fungus diseases and silborne fungal diseases, especially can prevent and treat leaf muld of tomato.
The present invention adopts following technical scheme:
Bacterial strain WS-8 of the present invention is bacillus amyloliquefaciens
bacillusamyloliquefaciens, preserving number is CGMCCNo.11787, and preservation date is on December 4th, 2015, depositary institution's title: China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101.
Bacillus amyloliquefaciens culture condition of the present invention is simple, on beef-protein medium flat board, bacterium colony is in white, circle, intermediate recess, smooth surface, opaque, and electric Microscopic observation thalline size is about 0.7 μm ~ 0.8 μm × 1.8 μm ~ 3 μm, raw in brood cell, oval; Gram-positive, facultative anaerobe; Physiological and biochemical index, in conjunction with the analysis of 16SrDNA, determines that this bacterial strain is bacillus amyloliquefaciens.
The Optimal compositions of fermentation medium formula of bacillus amyloliquefaciens WS-8 of the present invention is: starch 0.7%, soybean cake powder 1.6%, sucrose 0.2%, yeast extract paste 0.08%, peptone 0.05%, ammonium sulfate 0.15%, potassium primary phosphate 0.05%, magnesium sulfate 0.03%, manganous sulfate 0.01%, sodium-chlor 0.02%, calcium carbonate 0.1%, pH7.3-7.5, is more than mass percent.
Present invention also offers the application of bacillus amyloliquefaciens WS-8 in control crop blade face gas borne fungus diseases and silborne fungal diseases, particularly, described crop blade face gas borne fungus diseases is: black rot of pear, Pear black spot, gray mold of cucumber, leaf muld of tomato, graw mold of tomato, early blight of tomato or tomato sooty mold, and described silborne fungal diseases is damping-off, blight, verticillium or blight dis-ease.
Preferably, for preventing and treating crop blade face gas borne fungus diseases, spray in blade face after doubly being diluted by the fermented liquid 10-100 of described bacillus amyloliquefaciens, preferably 50 times of dilutions (spore count is 100,000,000/ml), spray once for 7-15 days.During for preventing and treating silborne fungal diseases, after doubly being diluted by the fermented liquid 10-100 of described bacillus amyloliquefaciens, fill with root, preferably 10 times of dilutions (spore count is 500,000,000/ml).
Present invention also offers described bacillus amyloliquefaciens WS-8 improving the application in crop physiology index and quality, being specially for improving crop leaf Determination of Chlorophyll content, soluble protein content and crop ascorbic acid content.Spray in crop after preferably doubly being diluted by the fermented liquid 10-100 of described bacillus amyloliquefaciens WS-8, preferably 50 times of dilutions (spore count is 100,000,000/ml), interval sprays once for 10 days.
Bacillus amyloliquefaciens WS-8 fermented liquid of the present invention dilutes 50 times of foliage-sprays, can reach 72% to the preventive effect of leaf muld of tomato; Can effectively improve leaf look simultaneously, improve the content of blade Determination of Chlorophyll; Improve the photosynthetic capacity of blade, promote the synthesis of photosynthate soluble proteins; Improve the content of xitix in tomato, improve Tomato Quality.The preventive effect of foliage-spray to canopy Indoor Airborne Fungi disease graw mold of tomato, early blight of tomato, gray mold of cucumber, powdery mildew of cucumber, cucumber downy mildew reaches 70.2%, 71%, 70.8%, 72%, 71.1% respectively; 64%, 69% is reached to the preventive effect of the ring spot and black spot of adopting rear pears; Fill with the preventive effect of root to the indoor tomato wilt of canopy and verticillium silborne fungal diseases and reach 61.8% and 62% respectively, also have good restraining effect to the streptococcus aureus of food source property.The efficient application of this bacterial strain visible effectively can reduce the sickness rate of fungal disease, and reduce the pesticide residue of product, protect the ecological safety of environment, its potential market has a extensive future.
Accompanying drawing explanation
Fig. 1 is the phylogenetic tree of bacillus amyloliquefaciens WS-8 of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is further described, so that those skilled in the art understand.
embodiment 1: the Isolation and Identification of bacillus amyloliquefaciens WS-8
Gather catch an illness and healthy vegetable blade in Luquan great river town, Shijiazhuang tomato, green cucumber canopy room, in this laboratory according to following CMC model, broad-spectrum antimicrobial bacillus amyloliquefaciens WS-8 of the present invention can be obtained:
The abstraction and purification of bacterial strain WS-8: by healthy sample and blade of catching an illness after removing rotten and mould, adopt the method for gradient dilution, 10 grams of blades are put into phosphoric acid buffer, room temperature 200r/min shakes 30min, supersound process 5min, obtained bacteria suspension spread plate, picking list bacterium colony adopts the method for line to carry out purifying.
Antagonistic strain WS-8 screens: by the single bacterium colony be purified into, and for the purpose of leaf muld of tomato, early blight, pathogenic strains carries out the primary dcreening operation of disease-resistant bacterial strain and multiple sieve; Obtain the bacterial strain WS-8 bacterial strain that antagonistic effect is good.
Bacillus amyloliquefaciens WS-8 of the present invention identifies according to the phylogenetic analysis of its colonial morphology and individual morphology feature, physiological and biochemical property and 16SrDNA gene order.
Individual morphology and the colony characteristics of this bacterial strain are as follows: electric Microscopic observation thalline size is about 0.7 μm ~ 0.8 μm × 1.8 μm ~ 3 μm, raw in brood cell, oval.Culture condition is simple, and on beef-protein medium flat board, bacterium colony is in white, and circle, intermediate recess, smooth surface, opaque, edge is irregular.
The part Physiology and biochemistry qualification result of this bacterial strain is as following table:
The Physiology and biochemistry qualification result of table 1 bacterial strain WS-8
。
The 16SrDNA gene order length of this bacterium is 1431bp, and utilize this sequence construct phylogenetic tree (shown in Fig. 1), determine the kind classification position of this bacterial strain, this bacterial strain WS-8 is bacillus amyloliquefaciens subspecies.
embodiment 2: the antimicrobial spectrum of bacillus amyloliquefaciens WS-8 measures
Bacterial strain of the present invention, with gas borne fungus diseases: black rot of pear, Pear black spot, gray mold of cucumber, leaf muld of tomato, graw mold of tomato, early blight of tomato, tomato sooty mold; Silborne fungal diseases: damping-off, blight, verticillium, blight dis-ease; Food borne bacteria: streptococcus aureus, multiple pathogenic strains carries out the mensuration of antimicrobial spectrum, the results are shown in Table 2.
The antimicrobial spectrum of table 2 bacillus amyloliquefaciens subspecies WS-8
Note: antagonistic action strong and weak with "+' ' represent."+": weak, antibacterial bandwidth is less than 1.5cm; " ++ ' ': medium tenacity, antibacterial bandwidth is between 1.5cm and 3.0cm; " +++ ": very strong, antibacterial bandwidth is greater than 3.0cm.
embodiment 3: the cultivation of bacillus amyloliquefaciens WS-8 and fermentation condition
Be purified into the bacterial strain that fungistatic effect is strong, adopt beef-protein medium, be kept on solid slope; Picking one ring from slant culture, is inoculated into 37 DEG C of incubated overnight in the liquid nutrient medium of beef extract-peptone, stand-by as bacterial classification seed bottle.
Fermentation culture conditions: fermention medium: starch 0.7%, soybean cake powder 1.6%, sucrose 0.2%, yeast extract paste 0.08%, peptone 0.05%, ammonium sulfate 0.15%, potassium primary phosphate 0.05%, magnesium sulfate 0.03%, manganous sulfate 0.01%, sodium-chlor 0.02%, calcium carbonate 0.1%, pH7.3-7.5, is more than mass percent.121 DEG C of sterilizings 20 minutes, after cooling, add in fermentation culture by seed liquor with inoculum size 1.5-2%, 600mL/3L triangular flask, rotating speed 180 turns/min, temperature 30 DEG C, cultivate 48 hours, obtain fermented liquid.
embodiment 4: bacillus amyloliquefaciens WS-8 prevents and treats the effect of leaf muld of tomato
In laboratory, adopt potted plant experiment, 5 process are set: blank (clear water process), fermented liquid 10 times dilution, fermented liquid 20 times dilution, fermented liquid 50 times dilution and fermented liquid 100 times dilution; After tomato the 3rd full exhibition of true leaf, 5 process spray the fermented liquid (amount of spraying all squirts even being as the criterion with blade tow sides) of clear water and different extension rate respectively, 48h inoculating tomato leaf mold pathogenic bacteria after spray, each process 24 basin, arranges three repetitions.Add up disease index and the protection effect (see table 3) of leaf muld of tomato after morbidity, integrated application cost and prevention effect, fermented liquid best applications concentration is 50 times of dilutions.
Leaf muld of tomato disease scale standard is as follows:
1 grade, blade is without scab;
2 grades, leaf margin and blade tip have scab, and scab is rare, lesion diameter D < 0.6cm;
3 grades, scab is many, but lesion diameter is 0.6≤D < 1.2cm; 4 grades, scab is many and in flakes, lesion diameter is 1.2cm≤D < 2.0cm; 5 grades, scab in flakes, lesion diameter D >=2.0cm.
Disease index calculation formula: disease index=100 × ∑ (diseased plant number at different levels × typical value at different levels)/(investigation blade amt × highest typical value).
The disease index of table 3 different weaker concn process tomato and prevention effect
。
embodiment 5: bacillus amyloliquefaciens subspecies WS-8 prevents and treats the Field information effect of leaf muld of tomato
Continuous 4 batches of application experiments preventing and treating leaf muld of tomato are carried out in tomato canopy room, Setup Experiments 4 process: blank, fermented liquid 50 times of dilutions, m-tetrachlorophthalodinitrile, many antibiotic, plastics film is adopted to separate between different treatment, to avoid influencing each other between different treatment, be convenient to experimental result statistics.Blank is left intact; Fermented liquid sprays to process and starts interval squaring period in tomato and within 10 days, spray once (amount of spraying all squirts even being as the criterion with blade face tow sides); M-tetrachlorophthalodinitrile, many antibiotic, according to the application experience of local vegetable grower, start to spray when morbidity sign appears in leaf muld of tomato, within 7-10 days, spray once afterwards according to state of an illness interval.When blank disease index reaches more than 50%, add up disease index and the protection effect (see table 4) of other process leafs muld of tomato.
The disease index of table 4 fermented liquid and pesticide-treated tomato and protection effect
。
embodiment 6: bacillus amyloliquefaciens WS-8 passes and the effect of silborne fungal diseases other gas
Carry out the prevention effect of bacillus amyloliquefaciens WS-8 to other gas biography and silborne fungal diseases respectively to test, wherein adopt 50 times to dilute fermented liquid for gas borne fungus diseases to spray, adopt 10 times to dilute fermentation liquid irrigating root for silborne fungal diseases to apply, every strain is filled with and is executed 10ml, each pathogenic bacteria is inoculated after using 48h, pendingly add up prevention effect after being ill, in table 5.
Table 5 bacillus amyloliquefaciens WS-8 passes and the prevention effect of silborne fungal diseases other gas
。
embodiment 7: bacillus amyloliquefaciens WS-8 is on the impact of tomato leaf physical signs and quality
In tomato canopy room, carry out the contrast application experiment of fermented liquid and chemical agent, arrange fermented liquid 50 times of dilutions, m-tetrachlorophthalodinitrile, the process of three, many antibiotic, fermented liquid sprays to process and starts interval squaring period in tomato and spray once in 10 days; M-tetrachlorophthalodinitrile, many antibiotic, according to the application experience of local vegetable grower, start to spray when morbidity sign appears in leaf muld of tomato, within 7-10 days, spray once, until the state of an illness is controlled afterwards according to state of an illness interval.After fermented liquid sprays 2 times, interval gets measuring blade chlorophyll and the soluble protein content of second leaf position (from vegetative point down several second leaf position) for 10 days; After Tomato Ripening, the tomato of getting the identical fruit ear position of different treatment measures ascorbic acid content; Tomato leaf and fruit measure the average result of 5 times in table 6, and the tomato that fermented liquid sprays process detects through Hebei province's quality supervised department, and quality reaches the standard of green vegetables.
Table 6 different treatment tomato leaf physical signs and quality of vegetable
。
SEQUENCELISTING
<110> Biology Inst., Hebei Academy of Sciences
<120> bacillus amyloliquefaciens WS-8 and application thereof
<160>1
<170>PatentInversion3.3
<210>1
<211>1431
<212>DNA
<213> bacillus amyloliquefaciens WS-8
<400>1
TGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAA60
CACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGA120
TGGTTGTTTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCGGCTACCACTTACAGATG180
GACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGC240
CGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG300
GCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTG360
ATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCCGTTCAAATAG420
GGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCG480
GTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGT540
TTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGA600
ACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGT660
GGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAG720
CGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAA780
GTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGG840
AGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGC900
ATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAAT960
CCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAG1020
CTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGC1080
CAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGAT1140
GACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAAC1200
AAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCG1260
CAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGC1320
GGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACAC1380
CCGAAGTCGGTGAGGTAACCTTTATGGAGCCAGCCGCCGAAGTGACAAGAT1431
Claims (8)
1. a bacillus amyloliquefaciens WS-8, it has carried out preservation on December 4th, 2015 at China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is CGMCCNo.11787.
2. bacillus amyloliquefaciens according to claim 1, is characterized in that its substratum consists of: starch 0.7%, soybean cake powder 1.6%, sucrose 0.2%, yeast extract paste 0.08%, peptone 0.05%, ammonium sulfate 0.15%, potassium primary phosphate 0.05%, magnesium sulfate 0.03%, manganous sulfate 0.01%, sodium-chlor 0.02%, calcium carbonate 0.1%, pH7.3-7.5, is more than mass percent.
3. the application of the bacillus amyloliquefaciens described in claim 1 or 2 in control crop blade face gas borne fungus diseases and silborne fungal diseases.
4. application according to claim 3, it is characterized in that: described crop blade face gas borne fungus diseases is: black rot of pear, Pear black spot, gray mold of cucumber, leaf muld of tomato, graw mold of tomato, early blight of tomato or tomato sooty mold, and described silborne fungal diseases is damping-off, blight, verticillium or blight dis-ease.
5. application according to claim 4, is characterized in that: spray in blade face after doubly being diluted by the fermented liquid 10-100 of described bacillus amyloliquefaciens or fill with root.
6. the bacillus amyloliquefaciens described in claim 1 or 2 is improving the application in crop quality.
7. application according to claim 6, is characterized in that: described bacillus amyloliquefaciens is for improving the ascorbic acid content in crop leaf Determination of Chlorophyll content, soluble protein content and crop.
8. application according to claim 7, is characterized in that: spray in crop after doubly being diluted by the fermented liquid 10-100 of described bacillus amyloliquefaciens, preferably 50 times of dilutions.
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Cited By (16)
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| CN105838656A (en) * | 2016-06-15 | 2016-08-10 | 东北农业大学 | Broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of broad-spectrum disease resisting, growth promotion and stress resisting bacillus |
| CN106399178A (en) * | 2016-10-08 | 2017-02-15 | 河北省农林科学院植物保护研究所 | Bacillus amyloliquefaciens with inorganic phosphorus degrading and bacteria inhibiting functions and application of bacillus amyloliquefaciens |
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| CN108991019A (en) * | 2018-07-06 | 2018-12-14 | 东北农业大学 | The biocontrol agent composition for preventing and treating graw mold of tomato and leaf mold |
| CN109006240A (en) * | 2018-07-16 | 2018-12-18 | 山东省农业科学院蔬菜花卉研究所 | A method of increase production for continuous cropping second crop soil melon crop promoting root growth |
| CN109294947A (en) * | 2018-10-08 | 2019-02-01 | 山东蔚蓝绿源生物科技有限公司 | A kind of separation screening and zymotechnique of the bacillus amyloliquefaciens for powdery mildew |
| CN111117908A (en) * | 2019-12-05 | 2020-05-08 | 上海万力华生物科技有限公司 | Bacillus amyloliquefaciens Ba0002 and application thereof |
| CN112322521A (en) * | 2020-10-23 | 2021-02-05 | 贵州大学 | Bacillus amyloliquefaciens GUOR0918 and application thereof in preventing and treating soft rot of kiwi fruits |
| CN114107130A (en) * | 2021-12-16 | 2022-03-01 | 天津科技大学 | Compound microbial fertilizer and preparation method and application thereof |
| CN114128724A (en) * | 2021-11-19 | 2022-03-04 | 天津开发区坤禾生物技术有限公司 | Application of bacillus amyloliquefaciens in tomato cultivation |
| CN114717165A (en) * | 2022-05-19 | 2022-07-08 | 河北省科学院生物研究所 | Complex microbial inoculant and biocontrol seed coating agent containing complex microbial inoculant |
| CN115322928A (en) * | 2022-08-10 | 2022-11-11 | 河北省科学院生物研究所 | Bacillus amyloliquefaciens ZLP-01 and application thereof |
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