CN111357771A - Biocontrol bacterium preparation for preventing and treating soil-borne diseases - Google Patents
Biocontrol bacterium preparation for preventing and treating soil-borne diseases Download PDFInfo
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- CN111357771A CN111357771A CN202010398143.8A CN202010398143A CN111357771A CN 111357771 A CN111357771 A CN 111357771A CN 202010398143 A CN202010398143 A CN 202010398143A CN 111357771 A CN111357771 A CN 111357771A
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- borne diseases
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The invention provides a biocontrol bacterium preparation for preventing and treating soil-borne diseases, which is prepared from the following raw materials in parts by weight: sargassum, Pelvetia siliquosa, Chlorella, Eucheuma Gelatinosum, Ulva pertusa, radix Rhodiolae, Bacillus amyloliquefaciens HAB-2, Bacillus subtilis, Trichoderma asperellum, yeast and Chaetomium aureofaciens. The invention can effectively prevent and control soil-borne diseases, improve soil quality and soil microorganism composition, greatly improve the growth condition of field crops and promote yield increase. Can prevent and control cucumber fusarium wilt, strawberry anthracnose, rice blast and other soil-borne diseases, and has important significance for the sustainable development of agricultural production.
Description
Technical Field
The invention belongs to the technical field of crop disease prevention and treatment, and particularly relates to a biocontrol bacterium preparation for preventing and treating soil-borne diseases.
Background
Soil-borne diseases are diseases caused by pathogens such as fungi, bacteria, nematodes and viruses that live in the soil with disease residues and, where appropriate, attack crops from their roots or stems. Has become an important obstacle factor for restricting the continuous development of facility agriculture. The infection pathogens comprise fungi, bacteria, actinomycetes, nematodes and the like, wherein the soil-borne pathogenic fungi are one of the main limiting factors for stable yield and high yield of crops and vegetables at present.
The Corn Stalk Rot (Corn Stalk Rot) is taken as an example to briefly summarize the economic loss caused by the Corn Stalk Rot, also called as Corn bacterial wilt, the Corn Stalk Rot is wide in distribution and serious in harm, all the Corn production areas in the world commonly occur, the annual incidence rate is 10-20%, and in serious cases, the annual incidence rate can reach more than 50%, so that the Corn yield is reduced by 20-30%. The main pathogenic bacteria causing the corn stalk rot are pythium, fusarium, dry rot, anthrax, bacteria and the like. Taking banana vascular wilt as an example, commonly called yellow leaf disease and Panama vascular wilt, banana is infected with the disease and then spreads quickly, and the number of diseased plants increases in geometric progression. Banana wilt is a devastating disease of bananas. Once the banana wilt generally reduces yield by more than 20 percent, serious field is even dead, so that a method for effectively preventing and controlling plant soil-borne diseases is imperative to be found.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides a biocontrol bacterium preparation for preventing and treating soil-borne diseases, which can effectively prevent and treat the soil-borne diseases and ensure the stable increase of the yield of crops and vegetables.
The invention provides a biocontrol bacterium preparation for preventing and treating soil-borne diseases, which is prepared from the following raw materials in parts by weight: 30-50 parts of chlorella, 30-50 parts of eucheuma, 10-30 parts of Ulva pertusa, 20-30 parts of rhodiola root, 21-5 parts of bacillus amyloliquefaciens HAB, 1-5 parts of bacillus subtilis, 1-3 parts of trichoderma asperellum, 1-3 parts of saccharomycetes and 1-3 parts of chaetomium aureocauda.
Preferably, the biocontrol bacterium preparation is prepared from the following raw materials in parts by weight: 40 parts of chlorella, 40 parts of eucheuma, 20 parts of Ulva pertusa, 25 parts of rhodiola root, 23 parts of Bacillus amyloliquefaciens HAB-2 parts of Bacillus subtilis, 2 parts of Trichoderma asperellum, 2 parts of microzyme and 2 parts of Chaetomium aureocauda.
In a second aspect of the present invention, there is provided a method for preparing the biocontrol bacteria preparation as described in the first aspect of the present invention, comprising the steps of:
pulverizing Chlorella, Eucheuma Gelatinosum, Ulva pertusa, and radix Rhodiolae, stirring, adding Bacillus amyloliquefaciens HAB-2, Bacillus subtilis, Trichoderma asperellum, yeast and Chaetomium aureofaciens, and fermenting for 3-30 days.
Furthermore, the temperature is controlled to be 15-35 ℃ in the fermentation process, and the fermentation time is 8-15 days.
Further, in the fermentation process, the humidity is maintained at 40-70%.
A third aspect of the present invention provides the use of a biocontrol agent as described in the first aspect of the invention for the control of soil-borne diseases.
Further, the soil-borne disease is cucumber fusarium wilt, strawberry anthracnose and/or rice blast.
The biocontrol bacterium preparation provided by the invention can effectively prevent and control crop soil-borne diseases, improve soil quality and soil microorganism composition, greatly improve the growth condition of field crops and promote yield increase. Proved by verification, the composition has obvious control effects on cucumber fusarium wilt, strawberry anthracnose and rice blast, and has important significance on sustainable development of agricultural production.
Detailed Description
The present invention will be better understood by reference to the following examples.
Example 1
A biocontrol bacterium preparation for preventing and treating soil-borne diseases is prepared from the following raw materials in parts by weight: 30 parts of chlorella, 30 parts of eucheuma, 10 parts of Ulva pertusa, 30 parts of rhodiola root, 21 parts of Bacillus amyloliquefaciens HAB-21 parts, 5 parts of Bacillus subtilis, 1 part of Trichoderma asperellum, 1 part of microzyme and 3 parts of Chaetomium aureocaudatum.
The preparation method comprises the following steps:
pulverizing Chlorella, Eucheuma Gelatinosum, Ulva pertusa, and radix Rhodiolae, stirring, adding Bacillus amyloliquefaciens HAB-2, Bacillus subtilis, Trichoderma asperellum, yeast and Chaetomium aureofaciens, and fermenting for 3-30 days.
Wherein, in the fermentation process, the humidity is maintained at 40-70%.
Wherein the temperature is controlled at 15 ℃ in the fermentation process, and the fermentation time is 15 days.
Example 2
A biocontrol bacterium preparation for preventing and treating soil-borne diseases is prepared from the following raw materials in parts by weight: 50 parts of chlorella, 50 parts of eucheuma, 30 parts of Ulva pertusa, 20 parts of rhodiola root, bacillus amyloliquefaciens HAB-25 parts, 1 part of bacillus subtilis, 3 parts of trichoderma asperellum, 3 parts of saccharomycetes and 1 part of chaetomium aureofaciens. And in the fermentation process, maintaining the humidity at 40-100%.
The preparation method comprises the following steps:
pulverizing Chlorella, Eucheuma Gelatinosum, Ulva pertusa, and radix Rhodiolae, stirring, adding Bacillus amyloliquefaciens HAB-2, Bacillus subtilis, Trichoderma asperellum, yeast and Chaetomium aureofaciens, and fermenting for 3-30 days.
Wherein, in the fermentation process, the humidity is maintained at 40-70%.
Wherein the temperature is controlled to be 35 ℃ in the fermentation process, and the fermentation time is 8 days.
Example 3
A biocontrol bacterium preparation for preventing and treating soil-borne diseases is prepared from the following raw materials in parts by weight: 40 parts of chlorella, 40 parts of eucheuma, 20 parts of Ulva pertusa, 25 parts of rhodiola root, 23 parts of Bacillus amyloliquefaciens HAB-2 parts of Bacillus subtilis, 2 parts of Trichoderma asperellum, 2 parts of microzyme and 2 parts of Chaetomium aureocauda.
The preparation method comprises the following steps:
pulverizing Chlorella, Eucheuma Gelatinosum, Ulva pertusa, and radix Rhodiolae, stirring, adding Bacillus amyloliquefaciens HAB-2, Bacillus subtilis, Trichoderma asperellum, yeast and Chaetomium aureofaciens, and fermenting for 3-30 days.
Wherein, in the fermentation process, the humidity is maintained at 40-70%.
Wherein the temperature is controlled to be 25 ℃ in the fermentation process, and the fermentation time is 10 days.
The biocontrol bacterium preparation provided by the invention is used for investigating the control effect of pathogenic bacteria
The crop seeds in the table 1 are disinfected on the surface, germinate in a culture dish with double-layer wet filter paper, are cultured for several days in a sterile water moisture-preserving way, are transplanted into sterile soil according to the conventional planting density after the seeds are degummed, and are cultured to cause diseases by irrigating corresponding pathogenic bacteria suspension to the roots of the seedlings when the seedlings grow a plurality of leaves. Biocontrol microbial agents were prepared according to the formulation of examples 1-3 and comparative examples 1-4, respectively, wherein the fermentation process of comparative examples 1-4 was performed according to the process of example 3.
TABLE 1 pathogenic bacteria and corresponding crop seeds
| Pathogenic bacteria | Crop seeds |
| Cucumber fusarium wilt | Cucumber seeds |
| Strawberry anthracnose pathogen | Strawberry seeds |
| Pyricularia oryzae of paddy rice | Rice seed |
Table 2 comparative example component ratios
After the crop seedlings are diseased, respectively scattering the same amount of the biocontrol bacterium preparation of the invention and the biocontrol bacterium preparation prepared by the comparative example on the surface of sterile soil, wherein the dosage is calculated according to the application of 80kg per mu, and then ploughing the biocontrol bacterium preparation into the sterile soil, and the control group is blank treatment. The control effect was observed after 15 days, and the results are shown in table 2.
Grading the disease condition standard:
grade 0, normal growth of the whole plant and no disease;
grade 1, a small number of lesions appear, and the area of the lesions accounts for below 1/4 of the total surface area of the affected part;
grade 2, the lesion area accounts for 1/4-1/2 of the total area of the root surface;
grade 3, wherein the area of the scab accounts for 1/2-3/4 of the total area of the root;
grade 4, the lesion area is greater than 3/4 of the total area of the root surface.
The disease index and the prevention and treatment effect of the strain are calculated as follows:
disease index [ ∑ (number of disease-grade plants x representative value of each grade)/(number of survey-total plants x highest representative value of the grade) ] x100
The prevention and treatment effect is [1- (disease index before prevention and treatment of a control group x disease index after prevention and treatment of a treatment group)/(disease index after prevention and treatment of a control group x disease index before prevention and treatment of a treatment group) ] x100
TABLE 3 controlling effect of the present invention on soil-borne diseases (%)
As can be seen from the above table, the biocontrol bacterium preparation of the invention can effectively control the soil-borne diseases, and the control effect is obviously higher than that of 4 comparative example treatment groups.
The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications and substitutions to those skilled in the art are also within the scope of the present invention. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (7)
1. A biocontrol bacterium preparation for preventing and treating soil-borne diseases is characterized by being prepared from the following raw materials in parts by weight: 30-50 parts of chlorella, 30-50 parts of eucheuma, 10-30 parts of Ulva pertusa, 20-30 parts of rhodiola root, 21-5 parts of bacillus amyloliquefaciens HAB, 1-5 parts of bacillus subtilis, 1-3 parts of trichoderma asperellum, 1-3 parts of saccharomycetes and 1-3 parts of chaetomium aureocauda.
2. The biocontrol bacteria preparation of claim 1, which is prepared from the following raw materials in parts by weight: 40 parts of chlorella, 40 parts of eucheuma, 20 parts of Ulva pertusa, 25 parts of rhodiola root, 23 parts of Bacillus amyloliquefaciens HAB-2 parts of Bacillus subtilis, 2 parts of Trichoderma asperellum, 2 parts of microzyme and 2 parts of Chaetomium aureocauda.
3. A method for producing the biocontrol bacterial preparation as defined in claim 1 or 2, comprising the steps of:
pulverizing Chlorella, Eucheuma Gelatinosum, Ulva pertusa, and radix Rhodiolae, stirring, adding Bacillus amyloliquefaciens HAB-2, Bacillus subtilis, Trichoderma asperellum, yeast and Chaetomium aureofaciens, and fermenting for 3-30 days.
4. The preparation method according to claim 3, wherein the temperature is controlled to be 15-35 ℃ during the fermentation process, and the fermentation time is 8-15 days.
5. The method according to claim 3, wherein the humidity is maintained at 40-70% during the fermentation process.
6. Use of a biocontrol bacterial formulation as defined in claim 1 or 2 for the control of soil-borne diseases.
7. The use of claim 7, wherein: the soil-borne diseases are cucumber fusarium wilt, strawberry anthracnose and/or rice blast.
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Cited By (3)
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| CN111807911A (en) * | 2020-07-27 | 2020-10-23 | 海南大学 | Soil microorganism regulator for enhancing plant stress resistance |
| CN112143684A (en) * | 2020-10-09 | 2020-12-29 | 吉林地富肥业科技有限责任公司 | Compound microbial inoculum for preventing and treating corn soil-borne diseases as well as preparation method and application thereof |
| CN115029253A (en) * | 2022-06-22 | 2022-09-09 | 湖南省农业环境生态研究所 | Application of chaetomium aureofaciens or spores thereof in resisting leaf spot disease of polygonatum |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111807911A (en) * | 2020-07-27 | 2020-10-23 | 海南大学 | Soil microorganism regulator for enhancing plant stress resistance |
| WO2022022204A1 (en) * | 2020-07-27 | 2022-02-03 | 海南大学 | Soil microbial regulator for enhancing plant stress resistance |
| CN112143684A (en) * | 2020-10-09 | 2020-12-29 | 吉林地富肥业科技有限责任公司 | Compound microbial inoculum for preventing and treating corn soil-borne diseases as well as preparation method and application thereof |
| CN115029253A (en) * | 2022-06-22 | 2022-09-09 | 湖南省农业环境生态研究所 | Application of chaetomium aureofaciens or spores thereof in resisting leaf spot disease of polygonatum |
| CN115029253B (en) * | 2022-06-22 | 2023-06-09 | 湖南省农业环境生态研究所 | Application of chaetomium aureum or spores thereof in resisting leaf spot disease of rhizoma polygonati |
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