CN110669677B - Penicillium bracteatum strain and application thereof - Google Patents
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Abstract
The invention discloses a penicillium bracteatum strain and application thereof. The Penicillium brevicornum strain is PFLJ04, the preservation name is Penicillium brevicornum brefeldianum, the Penicillium brevicornum is preserved in China general microbiological culture Collection center (CGMCC), the preservation unit address is No. 3 of Xilu No.1 of Beijing Korean area, the preservation date is as follows: 8, month and 15 days 2019; the preservation number is: CGMCC No. 18119. The penicillium is separated from the paris polyphylla endophyte, and can simultaneously have good inhibition effect on panax notoginseng rhizoctonia rot (fusarium oxysporum, fusarium solani and phytophthora infestans) and paris polyphylla leaf spot pathogenic bacteria and paris polyphylla gray mold (paris polyphylla gray mold, phoma stigmata leaf spot pathogenic bacteria and paris polyphylla pseudopiloniella).
Description
Technical Field
The invention relates to the technical field of microbial application, and particularly relates to a penicillium breyni strain and application thereof.
Background
In recent years, with the rapid increase of the demand of traditional Chinese medicine resources, the traditional Chinese medicine wild resources are seriously damaged, and the change of wild into cultivation becomes a necessary way for meeting the market demand. Wild traditional Chinese medicinal materials rarely cause diseases, even if the wild traditional Chinese medicinal materials do not cause disasters, the growth environment of the wild traditional Chinese medicinal materials is changed into artificial cultivation, the wild traditional Chinese medicinal materials are single in type and high in density in an agricultural ecological system, management measures such as high water and high fertilizer, artificial remote seed regulation and the like create conditions for epidemic diseases, the diseases are easy to spread and spread in the population, between fields and between regions, and the diseases of cultivated medicinal materials are aggravated.
Pseudo-ginseng (Panax notoginseng (Burk.) F.H.Chen) is a perennial herb of Panax of Araliaceae, and is a rare Chinese medicinal material in China. The pseudo-ginseng is perennial root plant, prefers warm and humid environment, and provides favorable conditions for the occurrence of diseases due to the single planting in large area in successive years in production. The main diseases of pseudo-ginseng are root rot, and the pathogenic bacteria of pseudo-ginseng mainly comprise: pillowcases (fungi, c.didymum), Fusarium solani (Fusarium solani), Fusarium oxysporum (Fusarium oxysporum), phoma (phoma herbare), rhizoctonia solani (rhizoctonia), phytophthora infestans (phytophthora parasiticum), Pseudomonas (Pseudomonas spp.), Meloidogyne (meloidogenin spp.), and the like. Root rot can cause the yield of pseudo-ginseng to be reduced by 5-20%, can reach more than 70% in serious cases, accounts for 70-85% of various diseases of pseudo-ginseng, and is one of the important restriction factors of the pseudo-ginseng planting industry at present.
Parisphylla rhizome (Parispyphylla. yunnanensis (Franch.) Hand) is a plant of the genus Trilliacea, is a raw material drug of famous Chinese patent medicines such as 'Yunnan white drug powder' and 'Gongxuening', and the rhizome contains steroid compounds which have the activities of hemostasis, anti-tumor, antimicrobial, immunoregulation and the like. After the wild paris polyphylla is changed into the domestic species, the living environment of the paris polyphylla is changed, the small species of various germs are changed, the weak small species are changed into the dominant small species, and new diseases are increased continuously. At present, the main diseases for cultivating the paris polyphylla include paris polyphylla Phoma spicatum (Phoma sp.), Botrytis cinerea (Botrytis cinerea), Alternaria tenuissima (Alternaria tenuissima) and the like.
At present, diseases of traditional Chinese medicinal materials are mainly prevented and controlled by chemical agents, long-term use of pesticides can cause environmental pollution, damage to an ecosystem, generation of drug resistance of pathogenic bacteria, and even cause harm such as pesticide residue and heavy metal pollution to the traditional Chinese medicinal materials, so that the use of the pesticides is reasonably reduced, a green and environment-friendly prevention and control mode is found, and the method has important significance for preventing and controlling the diseases of the traditional Chinese medicinal materials.
Disclosure of Invention
Aiming at the problems that the root rot of panax notoginseng and the fungal diseases of paris polyphylla are serious, the effect of preventing and controlling chemical pesticides is not ideal and environmental pollution is caused at the same time, a Penicillium brefeldianum strain for efficiently preventing and controlling the root rot of panax notoginseng, the gray mold of paris polyphylla and leaf spot and application thereof are provided.
In order to achieve the purpose, the invention adopts the following technical scheme: the Penicillium brevicornum strain is PFLJ04, the preservation name is Penicillium brevicornum brefeldianum, the Penicillium brevicornum is preserved in China general microbiological culture Collection center (CGMCC), the preservation unit address is No. 3 of Xilu No.1 of Beijing Korean area, the preservation date is as follows: 8, month and 15 days 2019; the preservation number is: CGMCC number 18119.
Further, the ITS gene sequence of the Penicillium breve strain is a nucleotide sequence shown in SEQ ID No. 1.
The penicillium brevicornum microbial inoculum prepared from the penicillium brevicornum strain is provided.
Further, the active ingredient is at least one of the following (a), (b) and (c):
(a) a fermentation culture of said penicillium brevicornum strain;
(b) ultrasonically cracking supernatant of the obtained penicillium brevicornum strain cells;
(c) ultrasonic cracking precipitation of the obtained penicillium bracteatum strain cells.
The preparation method of the fermentation liquor of penicillium brevicompactum comprises the following steps: inoculating the strain into a PDA culture medium plate, performing inverted culture at 28 ℃ for 7 days, activating the strain, preparing the activated strain into spore suspension by using distilled water, and counting the spore concentration under a microscope, wherein the spore suspension concentration is as follows: 1.0X 104-1.0×106Per mL; inoculating the spore suspension at 5% into liquid culture medium PDB, culturing at 28 deg.C and 200r/min for 7-10 days.
Further, the PDA culture medium consists of the following components: 100g-200g of potatoes, 5g-20g of glucose and 10g-20g of agar, diluting the mixture to a constant volume of 1L of distilled water, naturally adjusting the pH to 6-8, and sterilizing the mixture for 30min at 121 ℃; the PDB culture medium consists of the following components: 100g-200g of potatoes and 5g-20g of glucose, and the mixture is added into 1L of distilled water with constant volume, the pH value is 6-8, and the mixture is sterilized at 121 ℃ for 30 min.
The penicillium brevicornum strain of the invention is applied to the production of products for preventing and treating panax notoginseng root rot, paris polyphylla leaf spot disease or paris polyphylla gray mold.
The penicillium brevicornum fermentation liquid of the invention is applied to the production of products for preventing and treating panax notoginseng root rot, paris polyphylla leaf spot disease or paris polyphylla gray mold.
Further, the Penicillium bracteatum fermentation liquor is used for root irrigation treatment of the pseudo-ginseng or spraying treatment of the leaves of the Paris polyphylla.
Further, root irrigation can be carried out at seedling stage and adult stage of Notoginseng radix, strain fermentation liquid is diluted by 20 times and applied, each strain is used by 50ml, and the strain fermentation liquid is continuously used for 2-3 times, and each time is spaced by 30 days; can be sprayed on leaf surface in seedling stage and adult stage of rhizoma paridis, and the strain fermentation liquid is diluted by 20 times and continuously used for 2-3 times at an interval of 30 days.
Has the advantages that: the penicillium is separated from the paris polyphylla endophyte, and can simultaneously have good inhibition effect on panax notoginseng rhizoctonia rot (fusarium oxysporum, fusarium solani and phytophthora infestans) and paris polyphylla leaf spot pathogenic bacteria and paris polyphylla gray mold (paris polyphylla gray mold, phoma stigmata leaf spot pathogenic bacteria and paris polyphylla pseudopiloniella). Therefore, the strain fermentation liquor of the strain can effectively prevent and treat the root rot of panax notoginseng, the leaf spot of paris polyphylla and the gray mold of paris polyphylla.
Compared with the prior art, the invention has the following advantages:
(1) the bacterial strain PFLJ04 has broad-spectrum resistance, and can antagonize various pathogenic bacteria of panax notoginseng root rot and paris polyphylla. The field test shows that the strain has good control effect on the fusarium solani and the botrytis cinerea, can be developed into a high-efficiency biocontrol microbial inoculum, and effectively reduces the use of chemical pesticides.
Drawings
The following will be further explained in conjunction with the attached drawings, in which:
FIG. 1 shows the colony morphology of Penicillium brefeldianum.
FIG. 2 is a phylogenetic tree of Penicillium brefeldianum (NJ).
FIG. 3 shows the effect of confronting Penicillium brefeldianum (Penicillium brefeldianum) plates.
Detailed Description
The present invention is further illustrated in detail by the following examples, but it should be noted that the scope of the present invention is not limited by these examples at all.
Example 1
The Penicillium brevicornum strain is PFLJ04, the preservation name is Penicillium brevicornum (Penicillium brefeldianum), the Penicillium brevicornum strain is preserved in China general microbiological culture Collection center (CGMCC), the preservation unit address is No. 3 of Xilu No.1 Beichen of the sunward area in Beijing, the preservation date is as follows: 8, month and 15 days 2019; the preservation number is: CGMCC No. 18119.
The ITS gene sequence of the Penicillium breve strain is a nucleotide sequence shown in SEQ ID No. 1.
The penicillium brevicornum microbial inoculum prepared from the penicillium brevicornum strain is provided. The active component is at least one of the following components (a), (b) and (c):
(a) a fermentation culture of said penicillium brevicornum strain;
(b) ultrasonically cracking supernatant of the obtained penicillium brevicornum strain cells;
(c) ultrasonic cracking precipitation of the obtained penicillium bracteatum strain cells.
The preparation method of the fermentation liquor of penicillium brevicompactum comprises the following steps: inoculating the strain into a PDA culture medium plate, performing inverted culture at 28 ℃ for 7 days, activating the strain, preparing the activated strain into spore suspension by using distilled water, and counting the spore concentration under a microscope, wherein the spore suspension concentration is as follows: 1.0X 104Per mL; inoculating the spore suspension at 5% into liquid culture medium PDB, culturing at 28 deg.C and 200r/min for 10 days.
The PDA culture medium consists of the following components: 100g of potatoes, 20g of glucose and 18g of agar, diluting the mixture to 1L of distilled water, naturally adjusting the pH value, and sterilizing the mixture for 30min at 121 ℃; the PDB culture medium consists of the following components: 160g of potato and 16g of glucose, and the mixture is added into 1L of distilled water with constant volume, the pH value is 6, and the mixture is sterilized for 30min at 121 ℃.
The penicillium brevicornum strain of the invention is applied to the production of products for preventing and treating panax notoginseng root rot, paris polyphylla leaf spot disease or paris polyphylla gray mold.
The penicillium brevicornum fermentation liquid of the invention is applied to the production of products for preventing and treating panax notoginseng root rot, paris polyphylla leaf spot disease or paris polyphylla gray mold.
Root irrigation treatment is carried out on the pseudo-ginseng or spraying treatment is carried out on the leaves of the paris polyphylla by using the penicillium brevicornum fermentation liquor.
The strain fermentation liquor is diluted by 20 times and applied, wherein each strain is used for 50ml, and is continuously used for 2.5 times, and each time is separated by 30 days; can be sprayed on leaf surface in seedling stage and adult stage of rhizoma paridis, and the strain fermentation liquid is diluted by 20 times and continuously used for 2 times with 30 days interval.
Example 2
Example 2 differs from example 1 in that: the preparation method of the fermentation liquor of penicillium brevicompactum comprises the following steps: inoculating the strain into a PDA culture medium plate, performing inverted culture at 28 ℃ for 7 days, activating the strain, preparing the activated strain into spore suspension by using distilled water, and counting the spore concentration under a microscope, wherein the spore suspension concentration is as follows: 1.0X 106Per mL; the spore suspension was inoculated at 5% into liquid medium PDB, cultured at 28 ℃ at 200r/min for 7 days.
The PDA culture medium consists of the following components: 200g of potatoes, 5g of glucose and 10g of agar, diluting the mixture to 1L of distilled water, naturally adjusting the pH value, and sterilizing the mixture for 30min at 121 ℃; the PDB culture medium consists of the following components: 200g of potato and 5g of glucose, and the mixture is added into 1L of distilled water with constant volume, the pH value is 8, and the mixture is sterilized for 30min at 121 ℃.
The strain fermentation liquor is diluted by 20 times and applied, wherein each strain is used for 50ml, and the strain fermentation liquor is continuously used for 2 times at intervals of 30 days; can be sprayed on leaf surface in seedling stage and adult stage of rhizoma paridis, and the strain fermentation liquid is diluted by 20 times and continuously used for 3 times with 30 days interval.
Example 3
Example 3 differs from example 1 in that: the preparation method of the fermentation liquor of penicillium brevicompactum comprises the following steps: inoculating the strain into a PDA culture medium plate, performing inverted culture at 28 ℃ for 7 days, activating the strain, preparing the activated strain into spore suspension by using distilled water, and counting the spore concentration under a microscope, wherein the spore suspension concentration is as follows: 8.0X 104Per mL; inoculating the spore suspension at 5% into liquid culture medium PDB, culturing at 28 deg.C and 200r/min for 9 days.
The PDA culture medium consists of the following components: 180g of potatoes, 15g of glucose and 20g of agar, diluting the mixture to 1L of distilled water, naturally adjusting the pH value, and sterilizing the mixture for 30min at 121 ℃; the PDB culture medium consists of the following components: 100g of potato and 20g of glucose, and making the volume constant to 1L of distilled water, wherein the pH value is 7, and the potato is sterilized for 30min at 121 ℃.
The strain fermentation liquor is diluted by 20 times and applied, 50ml of the strain fermentation liquor is used for each plant, and the strain fermentation liquor is continuously used for 3 times at intervals of 30 days; can be sprayed on leaf surface in seedling stage and adult stage of rhizoma paridis, and the strain fermentation liquid is diluted by 20 times and continuously used for 2.5 times at an interval of 30 days.
Example 4
Obtaining of the strain:
penicillium brefeldianum PFLJ04, collecting healthy rhizoma paridis tubers from Yunnan province, soaking in 75% ethanol for 1min under conventional aseptic operation, washing with sterile water for 3 times, and drying surface water with sterile filter paper; 0.2% HgCl2 for 3min, washed with sterile water for 10 times, and blotted with sterile filter paper to remove surface water. Peeling rhizoma paridis tuber with surface sterilized by aseptic scalpel, cutting into 0.5cm cubes, placing in PDA culture medium plate, and culturing at 30 deg.C for 3-8 days. After the plate has bacteria, streaking and purifying the plate 3-5 times to obtain the strain PFLJ 04.
Example 5
And (3) strain identification:
1 morphological feature Observation
After culturing the strain PFLJ04 on PDA culture medium for 7-10 days, the colony is white, yellowish, with umbilical protrusion in the center and radial wrinkles on the surface (FIG. 1). The middle part is yellow in the back view, and the periphery is white. The hyphae have a septum, the conidiophores have branches to form a typical broom-shaped body, the conidiophores are oval, the walls of the conidiophores are provided with bulges, and the diameters of the conidiophores are 3-4 mu m.
2ITS sequence analysis
After extracting the genomic DNA of the strain by a liquid nitrogen grinding method, adopting a primer: ITS 1:
5'-TCCGTAGGTGAACCTGCGG-3'；ITS4：
5'-TCCTCCGCTTATTGATATGC-3', PCR amplification was performed. After gel electrophoresis, the gel is sent to bioengineering (Shanghai) Limited company for sequencing, and the total length of the sequence is 574bp (see SEQ). The obtained sequence was submitted to GenBank database for BLAST analysis and alignment, and the strain having the highest homology with PFLJ04 was Penicillium brefeldianum strain HMP-F96(NCBI accession No.: KM 243921.1). The ITS sequence was used to construct an NJ phylogenetic tree, with PFLJ04 branching in the same branch as Penicillium brefeldianum (FIG. 2). According to the morphological feature analysis, ITS analysis and NJ phylogenetic tree, the strain is preliminarily identified as the Penicillium brefeldianum group.
Example 6
Bacteriostatic activity of bacterial strain PFLJ04 on pathogenic bacteria of pseudo-ginseng and paris polyphylla
The bacterial strain PFLJ04 is prepared by using common panax notoginseng root rot fungi phytophthora cactorum (phytophthora cactorum), Fusarium Solani (Fusarium Solani), Fusarium oxysporum (Fusarium oxysporum) and common paris polyphylla pathogenic bacteria Botrytis cinerea (Botrytis cinerea), Phoma cladosporium (Phoma sp.) and Pestalotiopsis polyspora (Pestalotiopsis sp.) as pathogenic target bacteria through a PDA culture medium for 7 days, testing the bacteriostatic activity by adopting a mutual-confrontation culture method, and calculating the bacteriostatic rate: inhibition (%) - (control target colony diameter-treatment target colony diameter)/control target colony diameter × 100%. The results of the bacteriostatic activity of PFLJ04 on pathogenic bacteria of Notoginseng radix and rhizoma paridis are shown in Table 1.
TABLE 1
As shown in table 1, the experimental results of the present invention show that: the diameters of inhibition zones of the bacterial strain PBSZ123 for the pseudo-ginseng phytophthora infestans, the pseudo-ginseng fusarium solani, the pseudo-ginseng fusarium oxysporum, the paris polyphylla botrytis cinerea, the phoma longipes leaf spot germ and the paris polyphylla polyspora sp are respectively 20.2mm, 25.6mm, 22.3mm, 19.8mm, 21.8mm and 22.5mm on average. The inhibition rate of the strain PBSZ123 on the three pathogenic bacteria is more than 78.8-82.3%, which shows that the strain has good broad-spectrum antibacterial effect.
Example 7
Preparation of zymophyte liquid
Inoculating Penicillium bracteatum strain PFLJ04 into a PDA culture medium plate, performing inverted culture at 28 ℃ for 7 days, activating the strain, preparing the activated strain into a spore suspension by using distilled water, and counting the spore concentration under a microscope, wherein the spore suspension has the following concentration: 1.0X 104-1.0×106Per mL; inoculating the spore suspension at 5% into liquid culture medium PDB, culturing at 28 deg.C and 200r/min for 7-10 days.
Example 8
The Penicillium brefeldianum PFLJ04 fermented liquid has the effect of preventing and treating Fusarium solani.
1. Preparation of bacterial liquid of pathogenic bacteria of panax notoginseng root rot (Fusarium Solani): the fusarium solani activated on the PDA culture medium is scraped by a blade to be mycelium to be cultured on the liquid PDA culture medium (200r/min, 28 ℃) for 7 days to obtain the fusarium solani bacterial liquid of panax notoginseng.
2. And (4) selecting 1-year-old panax notoginseng seedlings to carry out root irrigation experiments. Experiment is as follows: CK group, using inoculated sterile distilled water as control 10 ml; in the SI group, only 10ml of pseudo-ginseng fusarium solani is inoculated; DI group: simultaneously inoculating 10ml of pseudo-ginseng fusarium solani and 10ml of penicillium brevicaulis. Each group is three times, and each time, 20 panax notoginseng seedlings are inoculated. After 14 days, the morbidity, disease index and prevention rate are counted.
3. The disease index and the prevention effect are calculated according to the following formulas
Disease index ∑ (number of diseased plant at each stage × disease-grade value)/(total number of investigated plants × highest-grade value) × 100
Severity grading criteria: level 0: no disease at root; level 1: the scab accounts for less than 20% of the surface area of the root; and 2, stage: the scab accounts for 21-40% of the surface area of the root; and 3, level: the spots account for 41% -65% of the root surface area, and the appearance is seriously affected; 4, level: lesions account for more than 66% of the surface area of the root or are completely rotten.
The preventing and treating rate (%) is (control disease index-treating disease index)/control disease index x 100
4. The control effect of Penicillium brefeldianum (Penicillium brefeldianum) PFLJ04 on panax notoginseng root rot is shown in table 2:
TABLE 2
| Treatment of | Incidence of disease | Index of disease condition | Rate of prevention and cure |
| CK | 0 | - | - |
| SI | 95.6％ | 62.5 | - |
| DI | 42.3％ | 28.9 | 53.7％ |
As shown in Table 2, the field experiment of the invention shows that the application of the strain for preventing and controlling the fusarium solani f.pseudo-ginseng can effectively reduce the disease index, the prevention and control rate of the fusarium solani f.pseudo-ginseng can reach 53.7%, and the strain can be used for preventing and controlling the fusarium solani f.pseudo-ginseng in the field.
Example 9
The control effect of Penicillium brefeldianum PFLJ04 fermentation liquor on gray mold of Paris polyphylla.
1. Preparation of a rhizoma paridis Botrytis cinerea (Botrytis cinerea) solution: scraping off mycelium of the paris polyphylla botrytis cinerea activated on the PDA culture medium by using a blade, and culturing the mycelium on a liquid PDA culture medium (180r/min, 28 ℃) for 7 days to obtain paris polyphylla botrytis cinerea bacterial liquid.
2. And (4) selecting 2-year-old paris polyphylla seedlings to carry out a foliage spraying experiment. Experiment is as follows: CK group, using inoculated sterile distilled water as control 10 ml; in the SI group, only the paris polyphylla gray mold liquid is inoculated; DI group: simultaneously inoculating the paris polyphylla gray mold bacterial liquid and the eupenicillium brevicornum. Each group is three times, and each time, 20 paris polyphylla seedlings are inoculated. After 14 days, the morbidity, disease index and prevention rate are counted.
3. The disease index and the prevention effect are calculated according to the following formulas
Disease index ∑ (number of diseased plants at each stage × disease grade value)/(total number of investigated plants × highest grade value) × 100
Severity grading criteria: severity grading criteria: level 0: no disease state; level 1: 1/4 leaf onset; and 2, stage: 1/2 leaf onset; and 3, level: the whole plant died.
The preventing and treating rate (%) is (control disease index-treating disease index)/control disease index x 100
4. The control effect of Penicillium brefeldianum (Penicillium brefeldianum) PFLJ04 on gray mold of paris is shown in table 3:
TABLE 3
As shown in Table 3, the field test of the invention shows that the application of the strain for preventing and controlling the gray mold of the paris polyphylla can effectively reduce the disease index, and the prevention rate of the gray mold of the paris polyphylla reaches 56.0 percent, which indicates that the strain can be used for preventing and controlling the gray mold of the paris polyphylla in the field.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the foregoing description only for the purpose of illustrating the principles of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the invention as defined by the appended claims, specification, and equivalents thereof.
SEQUENCE LISTING
<110> (institute of biotechnology and germplasm resources of academy of agricultural sciences of Yunnan province)
<120> penicillium brevicaulis strain and application thereof
<130> 2019
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 574
<212> DNA
<213> Artificial sequence (ITS region sequence of Penicillium brauense strain PFLJ 04)
<400> 1
ttttatacgg atgaggctct gggtcacctc ccacccgtgt ttatcgtacc ttgttgcttc 60
ggcgggcccg ccgttccggc cgccgggggg catccgcccc cgggcccgcg cccgccgaag 120
acaccattga acgctgtctg aagattgcag tctgagcgat tagctaaatc agttaaaact 180
ttcaacaacg gatctcttgg ttccggcatc gatgaagaac gcagcgaaat gcgataagta 240
atgtgaattg cagaattcag tgaatcatcg agtctttgaa cgcacattgc gccccctggt 300
attccggggg gcatgcctgt ccgagcgtca ttgctgccct caagcacggc tggtgtgttg 360
ggccccgccc cccggctacc ggggggcggg cccgaaaggc agcggcggca ccgcgtccgg 420
tcctcgagcg tatggggctt cgtcacccgc tctgtaggcc cggccggcgc ccgccggcga 480
cccccctcaa tctttctcag gttgacctcg gatcaggtag ggatacccgc tgaacttaag 540
catatcataa agacggagga aagatgtgaa atcc 574
Claims (7)
1. A penicillium breve strain characterized by: the Penicillium brevicornum strain is PFLJ04, the preservation name is Penicillium brefeldianum PFLJ04, the strain is preserved in China general microbiological culture Collection center (CGMCC), the preservation unit address is No. 3 of Xilu No.1 of Beijing Korean area, the preservation date is as follows: 8, month and 15 days 2019; the preservation number is: CGMCC number 18119.
2. The strain of penicillium brejust strain of claim 1, wherein: the ITS gene sequence of the Penicillium breve strain is a nucleotide sequence shown in SEQ ID No. 1.
3. The method for producing a fermentation broth of penicillium brevicornum according to claim 1, comprising the steps of: inoculating the strain into a PDA culture medium plate, performing inverted culture at 28 ℃ for 7 days, activating the strain, preparing the activated strain into spore suspension by using distilled water, and counting the spore concentration under a microscope, wherein the spore suspension concentration is as follows: 1.0X 104-1 .0×106Per mL; inoculating the spore suspension at 5% into liquid culture medium PDB, culturing at 28 deg.C and 200r/min for 7-10 days.
4. The method for producing a fermentation broth of penicillium brevicornum according to claim 3, wherein: the PDA culture medium consists of the following components: 100g-200g of potatoes, 5g-20g of glucose and 10g-20g of agar, diluting the mixture to a constant volume of 1L of distilled water, naturally adjusting the pH to 6-8, and sterilizing the mixture for 30min at 121 ℃; the PDB culture medium consists of the following components: 100g-200g of potatoes and 5g-20g of glucose, and the mixture is added into 1L of distilled water with constant volume, the pH value is 6-8, and the mixture is sterilized at 121 ℃ for 30 min.
5. Use of a strain of penicillium brevicornum according to any one of claims 1 to 2 for the manufacture of a product for the control of panax notoginseng root rot, paris polyphylla gray mold.
6. Use according to claim 5, characterized in that: root irrigation treatment is carried out on the pseudo-ginseng or spraying treatment is carried out on the leaves of the paris polyphylla by using the penicillium brevicornum fermentation liquor.
7. Use according to claim 5, characterized in that: the method can be used by irrigating roots at seedling stage and adult stage of Notoginseng radix, diluting strain fermentation liquid 20 times, using 50ml per plant, continuously using for 2-3 times at an interval of 30 days, spraying leaf surface at seedling stage and adult stage of rhizoma paridis, diluting strain fermentation liquid 20 times, continuously using for 2-3 times at an interval of 30 days.
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