CN110628686B - Bacillus polymyxa strain and application thereof - Google Patents
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- CN110628686B CN110628686B CN201911027540.8A CN201911027540A CN110628686B CN 110628686 B CN110628686 B CN 110628686B CN 201911027540 A CN201911027540 A CN 201911027540A CN 110628686 B CN110628686 B CN 110628686B
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Abstract
The invention discloses a bacillus polymyxa strain and application thereof. The bacillus polymyxa strain is PBSZ123, the preservation name is bacillus polymyxa (Paenibacillus polymyxa), the bacillus polymyxa strain is preserved in China general microbiological culture Collection center (CGMCC), the preservation unit address is No. 3 Beijing of West Lu No.1 of the Korean district in Beijing, and the preservation date is as follows: 8 month and 14 days 2019; the preservation number is: CGMCC No. 18386. The bacillus polymyxa is separated from the paris polyphylla endophyte, and has good inhibition effect on the main pathogenic bacteria of paris polyphylla botrytis cinerea, phoma stem leaf spot pathogen and paris polyphylla polyspora.
Description
Technical Field
The invention relates to the technical field of biological pesticides, and particularly relates to a bacillus polymyxa strain and application thereof.
Background
In recent years, with the rapid increase of the demand of traditional Chinese medicine resources, the traditional Chinese medicine wild resources are seriously damaged, and the change of wild into cultivation becomes a necessary way for meeting the market demand. Wild traditional Chinese medicinal materials rarely cause diseases, even if the wild traditional Chinese medicinal materials do not cause disasters, the growth environment of the wild traditional Chinese medicinal materials is changed into artificial cultivation, the wild traditional Chinese medicinal materials are single in type and high in density in an agricultural ecological system, management measures such as high water and high fertilizer, artificial remote seed regulation and the like create conditions for epidemic diseases, the diseases are easy to spread and spread in the population, between fields and between regions, and the diseases of cultivated medicinal materials are aggravated.
Parisphylla rhizome (Parispyphylla. yunnanensis (Franch.) Hand) is a plant of the genus Trilliacea, is a raw material drug of famous Chinese patent medicines such as 'Yunnan white drug powder' and 'Gongxuening', and the rhizome contains steroid compounds which have the activities of hemostasis, anti-tumor, antimicrobial, immunoregulation and the like. After the wild paris polyphylla is changed into the domestic species, the living environment of the paris polyphylla is changed, the small species of various germs are changed, the weak small species are changed into the dominant small species, and new diseases are increased continuously. The main diseases of the existing heavy storied buildings are as follows: paris polyphylla Phoma maculata (Phoma sp.), Botrytis cinerea (Botrytis cinerea), Alternaria tenuissima (Alternaria tenuissima), and the like.
At present, diseases of traditional Chinese medicinal materials are mainly prevented and controlled by chemical agents, long-term use of pesticides can cause environmental pollution, damage to an ecosystem, generation of drug resistance of pathogenic bacteria, and even cause harm such as pesticide residue and heavy metal pollution to the traditional Chinese medicinal materials, so that the use of the pesticides is reasonably reduced, a green and environment-friendly prevention and control mode is found, and the method has important significance for preventing and controlling the diseases of the traditional Chinese medicinal materials.
Disclosure of Invention
Aiming at the problems of serious fungus diseases of the paris polyphylla, unsatisfactory effect of preventing and controlling chemical pesticides and environmental pollution, the invention provides a bacillus polymyxa (Paenibacillus polymyxa) strain for efficiently preventing and controlling the gray mold and the leaf spot of the paris polyphylla and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme: the bacillus polymyxa strain is PBSZ123, the preservation name is bacillus polymyxa (Paenibacillus polymyxa), the bacillus polymyxa strain is preserved in China general microbiological culture Collection center (CGMCC), the preservation unit address is No. 3 Beijing of West Lu No.1 of the Korean district in Beijing, and the preservation date is as follows: 8 month and 14 days 2019; the preservation number is: CGMCC No. 18386.
Further, the gene sequence of the bacillus polymyxa strain is a 16S nucleotide sequence shown in SEQ ID No. 1.
The invention relates to a bacillus polymyxa microbial inoculum prepared from bacillus polymyxa strains.
Further, the active ingredient is at least one of the following (a), (b) and (c):
(a) a fermentation culture of said strain of Bacillus polymyxa;
(b) the ultrasonic cracking supernatant of the cells of the bacillus polymyxa strain;
(c) and carrying out ultrasonic lysis precipitation on the cells of the bacillus polymyxa strain.
The preparation method of the fermentation liquor of the bacillus polymyxa comprises the following steps:
inoculating the bacillus polymyxa strain into a beef extract peptone medium NA culture medium plate, carrying out inverted culture for 2 days at 28 ℃, activating the strain, selecting the strain, inoculating the strain into a beef extract peptone medium NA liquid culture medium, and culturing for 2 days at 28 ℃ and at the rotating speed of 200r/min to obtain the bacillus polymyxa fermentation liquid.
Further, the beef extract peptone medium NA medium consists of the following components: the mass ratio of the beef extract to the peptone to the NaCl to the agar is 5: 10: 5: 16; the mass volume ratio of the beef extract to the distilled water is 5 g: 1000mL, pH 6.5-6.8, autoclave sterilization at 121 ℃ for 20 min.
The application of the bacillus polymyxa strain PBSZ123 disclosed by the invention in production of products for preventing and treating paris polyphylla gray mold and leaf spot disease.
The invention relates to application of a bacillus polymyxa fermentation liquid in production of products for preventing and treating paris polyphylla gray mold.
Has the advantages that: the bacillus polymyxa is separated from the paris polyphylla endophyte, and has good inhibition effect on the main pathogenic bacteria of paris polyphylla botrytis cinerea, phoma stem leaf spot pathogen and paris polyphylla polyspora. Therefore, the strain fermentation liquor of the strain can effectively prevent and control the main diseases such as the leaf spot disease, the gray mold and the like of the paris polyphylla.
Compared with the prior art, the strain PBSZ123 has the advantages that (1) the strain PBSZ123 has the characteristic of broad-spectrum resistance to various paris polyphylla pathogenic bacteria, has good antagonistic effect, can be used as a biocontrol bacterium to prevent and control paris polyphylla fungal diseases, and effectively reduces the use of chemical pesticides.
Drawings
The following will be further explained in conjunction with the attached drawings, in which:
FIG. 1 shows the morphology of a Bacillus polymyxa PBSZ123 colony of the present invention.
FIG. 2 is a phylogenetic tree constructed by the Bacillus polymyxa PBSZ123NJ according to the 16S sequence.
FIG. 3 is a diagram showing the effect of the Bacillus polymyxa PBSZ123 plate facing each other.
Detailed Description
For a better understanding of the present invention, the following figures and examples further illustrate, but are not to be construed as limiting, the experimental procedures set forth in the following examples are conventional and are not intended to be limiting.
Example 1
The bacillus polymyxa strain is PBSZ123, the preservation name is bacillus polymyxa (Paenibacillus polymyxa), the bacillus polymyxa strain is preserved in China general microbiological culture Collection center (CGMCC), the preservation unit address is No. 3 Beijing of West Lu No.1 of the Korean district in Beijing, and the preservation date is as follows: 8 month and 14 days 2019; the preservation number is: CGMCC No. 18386.
The gene sequence of the bacillus polymyxa strain is a 16S nucleotide sequence shown in SEQ ID No. 1.
The invention relates to a bacillus polymyxa microbial inoculum prepared from bacillus polymyxa strains. The active component is at least one of the following components (a), (b) and (c):
(a) a fermentation culture of the bacillus polymyxa strain of claim 1;
(b) a sonicated supernatant of cells of the strain of Bacillus polymyxa of claim 1;
(c) ultrasonic lysis of cells of the strain of Bacillus polymyxa according to claim 1.
The preparation method of the fermentation liquor of the bacillus polymyxa comprises the following steps:
inoculating the bacillus polymyxa strain into a beef extract peptone medium NA culture medium plate, carrying out inverted culture for 2 days at 28 ℃, activating the strain, selecting the strain, inoculating the strain into a beef extract peptone medium NA liquid culture medium, and culturing for 2 days at 28 ℃ and at the rotating speed of 200r/min to obtain the bacillus polymyxa fermentation liquid.
The beef extract peptone medium NA medium consists of the following components: the mass ratio of the beef extract to the peptone to the NaCl to the agar is 5: 10: 5: 16; the mass volume ratio of the beef extract to the distilled water is 5 g: 1000mL, pH 6.5-6.8, autoclave sterilization at 121 ℃ for 20 min.
The application of the bacillus polymyxa strain PBSZ123 disclosed by the invention in production of products for preventing and treating paris polyphylla gray mold and leaf spot disease.
The invention relates to application of a bacillus polymyxa fermentation liquid in production of products for preventing and treating paris polyphylla gray mold.
Example 2
Obtaining of the strain:
the bacillus polymyxa PBSZ123 with the preservation number of CGMCC No.18386 is collected from Yunnan province, the tuber of the healthy rhizoma paridis is soaked in 75% ethanol for 1min under the conventional aseptic operation, the aseptic water is washed for 3 times, and the aseptic filter paper absorbs the surface moisture; 0.2% HgCl2 for 3min, washed with sterile water for 10 times, and blotted with sterile filter paper to remove surface water. Peeling the surface-sterilized rhizoma paridis tuber with sterile scalpel, cutting into 0.5cm cubes, placing in NA culture medium plate, and culturing at 28 deg.C for 2 days. After the plate has bacteria growing out, the plate is streaked and purified for 3-5 times to obtain the bacillus polymyxa PBSZ 123.
Example 3
And (3) strain identification:
1. morphological feature observation
And (3) colony morphology characteristics: the strain PBSZ123 colonies are light yellow on an NA plate, the surface is smooth and raised, and the edges are neat and round. Morphological characteristics of the thallus: gram-positive bacteria have spores, rods, and sizes (0.32-0.64) μm × (0.84-1.30) μm, as shown in FIG. 2.
2.16S sequence analysis
After extracting the genomic DNA of the strain by a liquid nitrogen grinding method, adopting a primer: 27F
5'-AGAGTTTGATCCTGGCTCAG3'；1492R
5'-TACGGCTACCTTGTTACGACTT-3' PCR amplification was performed.
After gel electrophoresis, the gel is sent to bioengineering (Shanghai) Limited company for sequencing, and the total length of the sequence is 1404bp (shown as SEQ). The resulting sequences were subjected to BLAST analysis alignment by submitting them to GenBank database, and it was found that the strain having a higher homology with PBSZ123 belongs to Bacillus polymyxa Paenibacillus polymyxa strain YC0136(NCBI accession No.: CP017967.3), and a NJ phylogenetic tree was constructed with the 16S sequence, PBSZ123 branching in the same branch as Paenibacillus polymyxa (FIG. 2).
According to the morphological feature analysis, ITS analysis and NJ phylogenetic tree, the strain is preliminarily identified as the bacillus polymyxa.
Example 4
Bacteriostatic activity of strain PBSZ123 on panax notoginseng pathogenic bacteria and paris polyphylla pathogenic bacteria
The strain PBSZ123 is cultured in an NA culture medium for 2 days, takes common pathogenic bacteria of rhizoma paridis, namely Botrytis cinerea (Botrytis cinerea), Phoma graminearum (Phoma sp.) and Pestalotiopsis sp.) as pathogenic target bacteria, tests the bacteriostatic activity by adopting a mutual confrontation culture method, and calculates the bacteriostatic rate: inhibition (%) - (control target colony diameter-treatment target colony diameter)/control target colony diameter × 100%. The results of the bacteriostatic activity of PFLJ04 against panax notoginseng pathogens and paris polyphylla pathogens are shown in table 1.
TABLE 1
As shown in table 1, the experimental results of the present invention show that: the average diameters of the inhibition zones of the strain PBSZ123 on the botrytis cinerea, the phoma longituba leaf spot pathogen and the pestalotiopsis paridis are 23.6mm, 24.5mm and 22.8mm respectively. The inhibition rate of the strain PBSZ123 on the three pathogenic bacteria is over 80 percent, which shows that the strain has good broad-spectrum antibacterial effect.
Example 5
Preparation of fermentation broth
Inoculating the bacillus polymyxa PBSZ123 into an NA culture medium plate, carrying out inverted culture for 2 days at 28 ℃, activating the strain, selecting the strain, inoculating the strain into 1000ml of a beef extract peptone culture medium NA liquid culture medium, and carrying out culture for 2 days at the temperature of 28 ℃ and the rotating speed of 200 r/min.
Example 6
The prevention and treatment effect of the bacillus polymyxa PBSZ123 fermentation liquor on the botrytis cinerea.
1. And (3) preparing a paris polyphylla gray mold bacterial liquid: scraping off mycelium from the Botrytis cinerea (Botrytis cinerea) activated on the PDA culture medium by using a blade, and culturing on a liquid PDA culture medium (180r/min, 28 ℃) for 7 days to obtain the Botrytis cinerea bacterial liquid.
2. And (4) selecting 2-year-old paris polyphylla seedlings to perform a control effect experiment. Experiment is as follows: CK group, using spray sterile distilled water as contrast; in the SI group, only paris polyphylla botrytis cinerea is sprayed; and (5) a DI group, spraying the bacillus polymyxa and spraying the paris polyphylla botrytis cinerea.
3. The disease index and the prevention effect are calculated according to the following formulas
Disease index ∑ (number of diseased plants at each stage × disease grade value)/(total number of investigated plants × highest grade value) × 100
Severity grading criteria: severity grading criteria: level 0: no disease state; level 1: 1/4 leaf onset; and 2, stage: 1/2 leaf onset; and 3, level: the whole plant died.
The preventing and treating rate (%) is (control disease index-treating disease index)/control disease index x 100
The control effect of bacillus polymyxa PBSZ123 on the botrytis cinerea is shown in Table 2:
TABLE 2
As shown in Table 2, field experiments show that the application of the strain for preventing and controlling the gray mold of paris polyphylla can effectively reduce disease indexes, the prevention and control rate of the gray mold of paris polyphylla reaches 65.8 percent, and the strain can be used for preventing and controlling the gray mold of paris polyphylla in fields.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the foregoing description only for the purpose of illustrating the principles of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the invention as defined by the appended claims, specification, and equivalents thereof.
SEQUENCE LISTING
<110> (institute of biotechnology and germplasm resources of academy of agricultural sciences of Yunnan province)
<120> bacillus polymyxa strain and application thereof
<130> 2019
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1404
<212> DNA
<213> Artificial series (16S nucleotide sequence of Bacillus polymyxa PBSZ 123)
<400> 1
tgcagtcgag cggggttgat tagaagcttg cttctaatca acctagcggc ggacgggtga 60
gtaacacgta ggcaacctgc ccacaagaca gggataacta ccggaaacgg tagctaatac 120
ccgatacatc cttttcctgc atgggagaag gaggaaagac ggagcaatct gtcacttgtg 180
gatgggcctg cggcgcatta gctagttggt ggggtaaagg cctaccaagg cgacgatgcg 240
tagccgacct gagagggtga tcggccacac tgggactgag acacggccca gactcctacg 300
ggaggcagca gtagggaatc ttccgcaatg ggcgaaagcc tgacggagca acgccgcgtg 360
agtgatgaag gttttcggat cgtaaagctc tgttgccagg gaagaacgtc ttgtagagta 420
actgctacaa gagtgacggt acctgagaag aaagccccgg ctaactacgt gccagcagcc 480
gcggtaatac gtagggggca agcgttgtcc ggaattattg ggcgtaaagc gcgcgcaggc 540
ggctctttaa gtctggtgtt taatcccgag gctcaacttc gggtcgcact ggaaactggg 600
gagcttgagt gcagaagagg agagtggaat tccacgtgta gcggtgaaat gcgtagatat 660
gtggaggaac accagtggcg aaggcgactc tctgggctgt aactgacgct gaggcgcgaa 720
agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgaatgct 780
aggtgttagg ggtttcgata cccttgggtg ccgaagttaa cacattaagc attccgcctg 840
gggagtacgg tcgcaagact gaaactcaaa ggaattgacg gggacccgca caagcagtgg 900
agtatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac atccctctga 960
tcggtctaga gatagacctt tccttcggga cagaggagac aggtggtgca tggttgtcgt 1020
cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct tatgcttagt 1080
tgccagcagg tcaagctggg cactctaagc agactgccgg tgacaaaccg gaggaaggtg 1140
gggatgacgt caaatcatca tgccccttat gacctgggct acacacgtac tacaatggcc 1200
ggtacaacgg gaagcgaaat cgcgaggtgg agccaatcct agaaaagccg gtctcagttc 1260
ggattgtagg ctgcaactcg cctacatgaa gtcggaattg ctagtaatcg cggatcagca 1320
tgccgcggtg aatacgttcc cgggtcttgt acacaccgcc cgtcacacca cgagagttta 1380
caacacccga agtcggtggg gtaa 1404
Claims (7)
1. A bacillus polymyxa strain characterized by: the bacillus polymyxa strain is PBSZ123, the preservation name is bacillus polymyxa (Paenibacillus polymyxa), the bacillus polymyxa strain is preserved in China general microbiological culture Collection center (CGMCC), the preservation unit address is No. 3 of the West Lu No.1 of the Chaoyang district in Beijing, and the preservation date is as follows: 8 month and 14 days 2019; the preservation number is: CGMCC No. 18386; the gene sequence of the bacillus polymyxa strain is a 16S nucleotide sequence shown in SEQ ID No. 1.
2. A Bacillus polymyxa bacterial agent produced from the Bacillus polymyxa strain of claim 1.
3. The Bacillus polymyxa inoculant according to claim 2, wherein the active ingredient is a fermentation culture of the Bacillus polymyxa strain of claim 1.
4. The method for producing a fermentation broth of a strain of bacillus polymyxa according to claim 1, comprising the steps of:
inoculating the bacillus polymyxa strain into a beef extract peptone medium NA culture medium plate, carrying out inverted culture for 2 days at 28 ℃, activating the strain, selecting the strain, inoculating the strain into a beef extract peptone medium NA liquid culture medium, and culturing for 2 days at 28 ℃ and at the rotating speed of 200r/min to obtain the bacillus polymyxa fermentation liquid.
5. The method for producing a fermentation broth of a strain of Bacillus polymyxa according to claim 4, wherein: the beef extract peptone medium NA medium consists of the following components: the mass ratio of the beef extract to the peptone to the NaCl to the agar is 5: 10: 5: 16; the mass volume ratio of the beef extract to the distilled water is 5 g: 1000mL, pH 6.5-6.8, autoclave sterilization at 121 ℃ for 20 min.
6. Use of the strain of bacillus polymyxa according to claim 1 for the production of products for the control of paris polyphylla gray mold and leaf spot.
7. The use of the method of claim 5 for the preparation of a fermentation broth of a strain of Bacillus polymyxa for the production of a product for the control of gray mold of Paris polyphylla.
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| CN110628686B (en) * | 2019-10-28 | 2021-05-11 | 云南省农业科学院生物技术与种质资源研究所 | Bacillus polymyxa strain and application thereof |
| CN111454860B (en) * | 2020-04-09 | 2022-01-18 | 广西乐土生物科技有限公司 | Bacillus polymyxa microbial inoculum and preparation method thereof |
| CN112662578A (en) * | 2020-11-10 | 2021-04-16 | 浙江省农业科学院 | Paenibacillus polymyxa DX32 and application thereof in inhibiting pestalotiopsis plant pathogenic fungi |
| CN113151079B (en) * | 2021-04-19 | 2022-05-17 | 河北省农林科学院谷子研究所 | Paenibacillus polymyxa KDB and application thereof |
| CN113151118A (en) * | 2021-06-02 | 2021-07-23 | 湖北省农业科学院中药材研究所 | Stenotrophomonas maltophilia for preventing and treating gray mold of paris polyphylla and application of stenotrophomonas maltophilia |
| CN113151119A (en) * | 2021-06-02 | 2021-07-23 | 湖北省农业科学院中药材研究所 | Paenibacillus terrestris for preventing and treating gray mold of paris polyphylla and application thereof |
| CN113151117A (en) * | 2021-06-02 | 2021-07-23 | 湖北省农业科学院中药材研究所 | Paenibacillus polymyxa for preventing and treating paris polyphylla gray mold and application thereof |
| CN113930364B (en) * | 2021-11-08 | 2023-07-07 | 怀化学院 | Bacillus and application and use method thereof for preventing and treating leaf spot of rhizoma polygonati phoma |
| CN114907986B (en) * | 2022-04-28 | 2022-11-18 | 云南省农业科学院生物技术与种质资源研究所 | Trichoderma harzianum and application thereof in preparation for preventing and treating root rot of panax notoginseng |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628686A (en) * | 2019-10-28 | 2019-12-31 | 云南省农业科学院生物技术与种质资源研究所 | Bacillus polymyxa strain and application thereof |
| CN110804566A (en) * | 2019-11-22 | 2020-02-18 | 汉江师范学院 | Bacillus for preventing and treating root rot of paris polyphylla and preparation method and application thereof |
| CN110839458A (en) * | 2019-11-28 | 2020-02-28 | 山东思远农业开发有限公司 | Comprehensive nematode prevention and control method in greenhouse |
| WO2020163251A1 (en) * | 2019-02-05 | 2020-08-13 | Pivot Bio, Inc. | Improved consistency of crop yield through biological nitrogen fixation |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020163251A1 (en) * | 2019-02-05 | 2020-08-13 | Pivot Bio, Inc. | Improved consistency of crop yield through biological nitrogen fixation |
| CN110628686A (en) * | 2019-10-28 | 2019-12-31 | 云南省农业科学院生物技术与种质资源研究所 | Bacillus polymyxa strain and application thereof |
| CN110804566A (en) * | 2019-11-22 | 2020-02-18 | 汉江师范学院 | Bacillus for preventing and treating root rot of paris polyphylla and preparation method and application thereof |
| CN110839458A (en) * | 2019-11-28 | 2020-02-28 | 山东思远农业开发有限公司 | Comprehensive nematode prevention and control method in greenhouse |
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