CN106804627B - A kind of complex microorganism Wettable pesticide powder and preparation method thereof and purposes - Google Patents
A kind of complex microorganism Wettable pesticide powder and preparation method thereof and purposes Download PDFInfo
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- CN106804627B CN106804627B CN201710067939.3A CN201710067939A CN106804627B CN 106804627 B CN106804627 B CN 106804627B CN 201710067939 A CN201710067939 A CN 201710067939A CN 106804627 B CN106804627 B CN 106804627B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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Abstract
The invention discloses a kind of complex microorganism Wettable pesticide powders, it is made of the component of following mass percent: golden yellow vertical streptomycete fermentation object 10%~15%, rose yellow streptomycete mycelia 10%~15%, actinomyces styreptomyces globispotus strain original powder 10%~15%, Wuyi bacterium conidia powder 10%~15%, bacillus mycelia 10%~15%, choline chloride 0.5%~2%, light rare earth 0.5%~2%, vitamins 0.5%~2%, microelement 0.5%~2%, wetting agent 2%~5%, dispersing agent 2%~5%, stabilizer 2%~5%, filler is supplied to 100%.Complex microorganism Wettable pesticide powder composition collocation science of the invention, compatibility are reasonable；Has a broad antifungal spectrum, activity is high, all has good preventing disease theraping effect to many crop diseases, control cost is effectively reduced；Environmental pollution is small, has nuisanceless and the characteristics of without residual hazard, effectively prevents the harm of chemical pesticide.In addition, the invention discloses the Preparation method and uses of the complex microorganism Wettable pesticide powder.
Description
Technical field
The present invention relates to a kind of complex microorganism Wettable pesticide powder and preparation method thereof and purposes, belong to pesticide neck
Domain.
Background technique
The domestic prevention and treatment for plant especially corps diseases is substantially using chemical pesticide at present, in chemical pesticide
In the case that drawback is increasingly prominent, some microbial pesticides gradually receive the highest attention of domestic and foreign scholars.Microbial source
The exploitation and application of pesticide have been increasingly becoming the new trend of pesticide industry, and main advantage is: 1) many kinds of, the choosing of research and development
It is big to select leeway；2) specificity is strong, to non-target organism safety, is not likely to produce drug resistance；3) activity is high, and production cost is low, fermentation
Simple process；4) environmentally safe, nuisanceless, noresidue.
But the pesticide of existing microbial source can be only applied to the prevention and control of a kind of or a kind of disease, application limitation is very
Greatly, peasant also wishes that a kind of pesticide as much as possible can be suitable for a variety of diseases, rather than a kind of pesticide can be only applied to one
Kind or a kind of disease, therefore, develop a kind of safety, low toxicity, stablize, efficiently, the microbial pesticide of has a broad antifungal spectrum compels in eyebrow
Eyelash.
Summary of the invention
It is an object of the invention to develop a kind of complex microorganism Wettable pesticide powder, the microbial pesticide release
Rate is high, and has a broad antifungal spectrum can efficiently prevent and cure diseases, and safe, low toxicity, the nutriment containing there are many, is conducive to crop yield, can grow
Phase, which stablizes, to be saved.
To achieve the above object, the technical scheme adopted by the invention is as follows: a kind of complex microorganism Wettable pesticide powder,
It is made of the component of following mass percent: golden yellow vertical streptomycete fermentation object 10%~15%, rose yellow streptomycete mycelia
10%~15%, actinomyces styreptomyces globispotus strain original powder 10%~15%, Wuyi bacterium conidia powder 10%~15%, bacillus mycelia
10%~15%, choline chloride 0.5%~2%, light rare earth 0.5%~2%, vitamins 0.5%~2%, microelement
0.5%~2%, wetting agent 2%~5%, dispersing agent 2%~5%, stabilizer 2%~5%, filler are supplied to 100%.
Preferably, the deposit number of golden yellow vertical streptomycete is CCTCC NO.M2010293, and the preservation of rose yellow streptomycete is compiled
It number is CGMCC NO.1471, the deposit number of actinomyces styreptomyces globispotus strain is CCTCC NO.M 2011012, and bacillus is ground
Clothing bacillus, bacillus subtilis or wax bacillus.
Wherein choline chloride, light rare earth, vitamin etc. are conducive to plant stress-resistance growth promoting, have apparent disease resistance, resist
It is inverse, improve crop yield, improve crop product quality effect, this formula under microbial pesticide in Microbiological release
Rate is high, has a broad antifungal spectrum, and safe and environment-friendly.
Preferably, the golden yellow vertical streptomycete fermentation object is prepared by following steps: 1) activate golden yellow vertical streptomycete:
Golden yellow vertical streptomycete is seeded on Gause I culture medium flat plate with method of scoring, 28 DEG C constant temperature incubation 4 days, the matter of culture medium
Measure percentage composition: soluble starch 2.5%, KNO30.2%, K2HPO40.05%, MgSO40.05%, NaCl
0.05%, FeSO40.05%, agar 2.5%, moisturizing to 100%；2) the golden yellow vertical streptomycete of activation is inoculated in level-one kind
In sub- culture solution, 180rmin-1, 28 DEG C of culture 72h, the mass percentage composition of first order seed culture solution: soluble starch
2.5%, KNO30.2%, K2HPO40.05%, MgSO40.05%, NaCl 0.05%, FeSO40.05%, moisturizing is extremely
100%；3) the first order seed culture solution prepared is seeded in secondary seed culture solution, 180rmin-1, 28 DEG C of cultures
72h, centrifuge separation obtain golden yellow vertical streptomycete fermentation object, low temperature drying, and moisture content is lower than 5%, secondary seed culture solution
Mass percentage composition: glucose 0.05%, soluble starch 0.05%, K2HPO40.05%, NaCl 0.05%, MgSO4
0.05%, peptone 0.05%, yeast extract 0.05% and soybean powder 0.05%, moisturizing to 100%.
Preferably, the rose yellow streptomycete mycelia is prepared by following steps: 1) being cultivated rose yellow streptomycete in PDA
It is cultivated 5 days for 28 DEG C on base；2) it is then inoculated into seed culture medium with 2% ratio, condition of culture are as follows: 28 DEG C, 180r
min-1, cultivate 3 days, wherein seed culture medium are as follows: and 3.5% groundnut meal, 5% starch, 1% glucose, 0.8% sodium chloride,
0.3% alcohol mother's cream, 0.8% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.4% calcium carbonate, 0.2% ferrous sulfate, 0.2% sulfuric acid
Zinc, 3.5% sucrose, 0.4% fructose；3) the rose yellow streptomycete culture that step (2) is prepared is by being obtained by filtration
State rose yellow streptomycete mycelia.
Preferably, the actinomyces styreptomyces globispotus strain original powder is prepared by following steps: 1) by activated actinomyces
Styreptomyces globispotus strain is inoculated into 2% ratio is inoculated in Gause I fluid nutrient medium, and 28 DEG C, 150-160rmin-1Oscillation training
Actinomyces seed culture fluid is obtained after supporting 120h；2) the actinomyces styreptomyces globispotus strain that step (2) is prepared is by filtering, doing
It is dry to obtain the actinomyces styreptomyces globispotus strain original powder.
Preferably, the bacillus mycelia is prepared by following steps: activated bacillus is pressed to 2% ratio
Example is inoculated in culture medium, is cultivated 5 days at 28 DEG C, is taken out culture and is filtered, obtained solid is bacillus bacterium
Silk；Wherein the bacillus is bacillus licheniformis, bacillus subtilis or wax bacillus.
Preferably, the complex microorganism Wettable pesticide powder is made of the component of following mass percent: golden yellow to hang down
Straight chain mold fermentation object 10%~12%, rose yellow streptomycete mycelia 10%~12%, actinomyces styreptomyces globispotus strain original powder 10%
~12%, Wuyi bacterium conidia powder 10%~12%, bacillus mycelia 10%~12%, choline chloride 0.5%~1%, light dilute
Soil 0.5%~1%, vitamins 0.5%~1%, microelement 0.5%~1%, wetting agent 2%~4%, dispersing agent 2%~
4%, stabilizer 2%~4%, filler are supplied to 100%.This formula under pesticide wetability, dispersibility, suspension, granulating rate,
High stability, and preventing disease theraping effect is preferable.
It is furthermore preferred that the complex microorganism Wettable pesticide powder is made of the component of following mass percent: golden yellow
Vertical streptomycete fermentation object 10%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 10%, Wuyi bacterium spore
Powder 10%, bacillus mycelia 10%, choline chloride 0.5%, light rare earth 0.5%, vitamins 1%, microelement 1%, profit
Humectant 3%, dispersing agent 3%, stabilizer 3%, filler are supplied to 100%.This is formulated lower complex microorganism Wettable pesticide powder
Wetability, dispersibility, suspension, granulating rate, stability be all up optimum level, after measured, complex microorganism wettable powder
It can be stored 6 months under the shelf storage life room temperature of agent pesticide, bacteriostasis rate is positively retained at 85% or more, and preventing disease theraping effect is most
It is excellent.
Preferably, the vitamins is at least one of vitamin, B or C.It is furthermore preferred that the vitamins is
The mixture of vitamin B and C, the mass ratio of the two are 1:1.
Preferably, the microelement is B, Mn, Mo, Cu, Zn and Fe.Preferred described B, Mn, Mo, Cu, Zn and Fe
Mass ratio be 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, manganese sulfate, sodium molybdate, sulphur
Sour copper, zinc sulfate, ferrous sulfate.When using the microelement of the quality proportioning, the complex microorganism wettable powder
Pesticide performs to utmostly the production-increasing function of crops.
Preferably, the wetting agent is lauryl sodium sulfate, Tween-80, at least one in sodium methylene bis-naphthalene sulfonate
Kind；The dispersing agent is at least one of sodium lignin sulfonate, sodium lignin sulfonate calcium, poly-aspartic-acid；The stabilization
Agent is at least one of vitamin E, potassium dihydrogen phosphate；The filler is diatomite, bentonite, at least one in kaolin
Kind.
It is furthermore preferred that the wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, wherein the matter of the two
Amount is than being 1:1；The dispersing agent is sodium lignin sulfonate calcium；The stabilizer is vitamin E；The filler is kaolin and silicon
The mixture of diatomaceous earth, wherein the mass ratio of the two is 1:2.
Preferably, a kind of complex microorganism Wettable pesticide powder of the present invention, by the group of following mass percent
Divide and be made: golden yellow vertical streptomycete fermentation object 15%, rose yellow streptomycete mycelia 12%, actinomyces styreptomyces globispotus strain original powder
15%, Wuyi bacterium conidia powder 12%, bacillus mycelia 15%, choline chloride 1%, light rare earth 2%, vitamins 2%, micro
Element 1%, wetting agent 4%, dispersing agent 4%, stabilizer 4%, filler are supplied to 100%；Wherein vitamins be vitamin B and
The mixture of C, the mass ratio of the two are 1:1；Microelement is B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo, Cu, Zn and Fe
Mass ratio be 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, manganese sulfate, sodium molybdate, sulphur
Sour copper, zinc sulfate, ferrous sulfate；Wetting agent is sodium methylene bis-naphthalene sulfonate；Dispersing agent is poly-aspartic-acid；Stabilizer is dimension
Mixture in raw element E and potassium dihydrogen phosphate, wherein the mass ratio of the two is 1:1；Filler is bentonite and kaolinic mixing
Object, wherein the mass ratio of the two is 1:1.
It is furthermore preferred that component system of the complex microorganism Wettable pesticide powder of the present invention by following mass percent
At: golden yellow vertical streptomycete fermentation object 10%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 10%, force
Smooth bacterium conidia powder 10%, bacillus mycelia 10%, choline chloride 0.5%, light rare earth 0.5%, vitamins 1%, micro member
Element 1%, wetting agent 3%, dispersing agent 3%, stabilizer 3%, filler are supplied to 100%；Wherein the vitamins is vitamin B
With the mixture of C, the mass ratio of the two is 1:1:1；The microelement be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo,
The mass ratio of Cu, Zn and Fe be 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, manganese sulfate,
Sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate,
Both middle mass ratio is 1:1；Dispersing agent is calcium lignosulfonate；Stabilizer is vitamin E；The filler is kaolin and silicon
The mixture of diatomaceous earth, wherein the mass ratio of the two is 1:2.
In addition, the present invention discloses a kind of preparation method of complex microorganism Wettable pesticide powder described above, packet
It includes following steps: wetting agent, dispersing agent, stabilizer, filler, choline chloride, light rare earth being put into air-flow according to the ratio
It is crushed in pulverizer, crosses 350 meshes, after cooling, golden yellow vertical streptomycete fermentation object, rose yellow streptomycete mycelia, unwrapping wire is added
Bacterium styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder, bacillus mycelia, vitamins, microelement, continue to crush, obtain institute
State complex microorganism Wettable pesticide powder.
In addition, the present invention discloses one kind complex microorganism Wettable pesticide powder described above withered in prevention and treatment banana
Purposes in disease, winter jujube rust, cotton verticillium wilt and peanut yield increasing.
The beneficial effects of the present invention are: complex microorganism Wettable pesticide powder composition collocation science of the invention is matched
5 rationally；Has a broad antifungal spectrum, activity is high, all has good preventing disease theraping effect to many crop diseases, be effectively reduced prevention and treatment at
This；Lower production costs favorably improve the competitiveness of product in market；Environmental pollution is small, there is spy nuisanceless and without residual hazard
Point effectively prevents the harm of chemical pesticide.
Specific embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of complex microorganism Wettable pesticide powder, is made of the component of following mass percent: golden yellow vertical chain
Mold fermentation object 10%, rose yellow streptomycete mycelia 15%, actinomyces styreptomyces globispotus strain original powder 10%, Wuyi bacterium conidia powder
15%, bacillus mycelia 10%, choline chloride 2%, light rare earth 0.5%, vitamins 0.5%, microelement 2%, wetting
Agent 2%, dispersing agent 5%, stabilizer 5%, filler are supplied to 100%.
Wherein, the vitamins is vitamin C；The microelement be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn,
The mass ratio of Mo, Cu, Zn and Fe are 4:5:2:5:2:7, and B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, sulfuric acid
Manganese, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is lauryl sodium sulfate；The dispersing agent is sulfomethylated lignin
Sour sodium；The stabilizer is vitamin E；The filler is diatomite.
The complex microorganism Wettable pesticide powder of the present embodiment is prepared using following methods: according to above-mentioned recipe ratio
Wetting agent, dispersing agent, stabilizer, filler, choline chloride, light rare earth are put into airslide disintegrating mill and are crushed by example, cross 350 mesh
After cooling, golden yellow vertical streptomycete fermentation object, rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder, Wuyi is added in sieve
Bacterium conidia powder, bacillus mycelia, vitamins, microelement continue to crush to get the complex microorganism wettable powder is arrived
Agent pesticide；
Wherein, golden yellow vertical streptomycete fermentation object is prepared by following steps: 1) activating golden yellow vertical streptomycete: to cross
Golden yellow vertical streptomycete is seeded on Gause I culture medium flat plate by method, 28 DEG C constant temperature incubation 4 days, the quality percentage of culture medium
Than composition: soluble starch 2.5%, KNO30.2%, K2HPO40.05%, MgSO40.05%, NaCl 0.05%,
FeSO40.05%, agar 2.5%, moisturizing to 100%；2) the golden yellow vertical streptomycete of activation is inoculated in first order seed culture
In liquid, 180rmin-1, 28 DEG C of culture 72h, the mass percentage composition of first order seed culture solution: soluble starch 2.5%, KNO3
0.2%, K2HPO40.05%, MgSO40.05%, NaCl 0.05%, FeSO40.05%, moisturizing to 100%；It 3) will preparation
Good first order seed culture solution is seeded in secondary seed culture solution, 180rmin-1, 28 DEG C of culture 72h, centrifuge separation, obtain
Golden yellow vertical streptomycete fermentation object, low temperature drying, moisture content are lower than 5%, the mass percentage composition of secondary seed culture solution:
Glucose 0.05%, soluble starch 0.05%, K2HPO40.05%, NaCl 0.05%, MgSO40.05%, peptone
0.05%, yeast extract 0.05% and soybean powder 0.05%, moisturizing to 100%.
Wherein, rose yellow streptomycete mycelia is prepared by following steps: 1) by rose yellow streptomycete in PDA culture medium 28
DEG C culture 5 days；2) it is then inoculated into seed culture medium with 2% ratio, condition of culture are as follows: 28 DEG C, 180rmin-1, culture
3 days, wherein seed culture medium are as follows: 3.5% groundnut meal, 5% starch, 1% glucose, 0.8% sodium chloride, 0.3% alcohol mother's cream,
0.8% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.4% calcium carbonate, 0.2% ferrous sulfate, 0.2% zinc sulfate, 3.5% sucrose,
0.4% fructose；3) the rose yellow streptomycete culture that step (2) is prepared is by being obtained by filtration the rose yellow streptomycete
Mycelia.
Wherein, actinomyces styreptomyces globispotus strain original powder is prepared by following steps: 1) by activated actinomyces ball spore chain
Mould is inoculated into 2% ratio is inoculated in Gause I fluid nutrient medium, and 28 DEG C, 150-160rmin-1Shaken cultivation 120h
Actinomyces seed culture fluid is obtained afterwards；2) the actinomyces styreptomyces globispotus strain that step (2) is prepared passed through into filtering, be dried to obtain
The actinomyces styreptomyces globispotus strain original powder.
Wherein, bacillus mycelia is prepared by following steps: activated bacillus is inoculated in 2% ratio
It in culture medium, is cultivated 5 days at 28 DEG C, takes out culture and be filtered, obtained solid is bacillus mycelia；Wherein
The bacillus is bacillus licheniformis, bacillus subtilis or wax bacillus.
Embodiment 2
A kind of complex microorganism Wettable pesticide powder, is made of the component of following mass percent: golden yellow vertical chain
Mold fermentation object 12%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 12%, Wuyi bacterium conidia powder
10%, bacillus mycelia 12%, choline chloride 0.5%, light rare earth 1%, vitamins 1%, microelement 0.5%, wetting
Agent 5%, dispersing agent 2%, stabilizer 2%, filler are supplied to 100%.
The vitamins is vitamin B；The microelement be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo, Cu,
The mass ratio of Zn and Fe is 4:5:2:5:2:7, and B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, manganese sulfate, molybdenum
Sour sodium, copper sulphate, zinc sulfate, ferrous sulfate；Both wetting agent is the mixture of lauryl sodium sulfate and Tween-80, wherein
Mass ratio be 1:2；Dispersing agent is the mixture of sodium lignin sulfonate and sodium lignin sulfonate calcium, wherein the mass ratio of the two is
1:1；Stabilizer is potassium dihydrogen phosphate；Filler is bentonite.
The complex microorganism Wettable pesticide powder of the present embodiment is prepared using preparation method as described in Example 1.
Embodiment 3
A kind of complex microorganism Wettable pesticide powder, is made of the component of following mass percent: golden yellow vertical chain
Mold fermentation object 15%, rose yellow streptomycete mycelia 12%, actinomyces styreptomyces globispotus strain original powder 15%, Wuyi bacterium conidia powder
12%, bacillus mycelia 15%, choline chloride 1%, light rare earth 2%, vitamins 2%, microelement 1%, wetting agent
4%, dispersing agent 4%, stabilizer 4%, filler are supplied to 100%.
Vitamins is the mixture of vitamin B and C, and the mass ratio of the two is 1:1；Microelement be B, Mn, Mo, Cu,
Zn and Fe, wherein the mass ratio of B, Mn, Mo, Cu, Zn and Fe are 4:5:2:5:2:7, and B, Mn, Mo, Cu, Zn and Fe are from following
Raw material: borax, manganese sulfate, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is sodium methylene bis-naphthalene sulfonate；Point
Powder is poly-aspartic-acid；Stabilizer is the mixture in vitamin E and potassium dihydrogen phosphate, wherein the mass ratio of the two is 1:
1；Filler is bentonite and kaolinic mixture, wherein the mass ratio of the two is 1:1.The complex microorganism of the present embodiment is wettable
Property powder pesticide be prepared using preparation method as described in Example 1.
Embodiment 4
A kind of complex microorganism Wettable pesticide powder, is made of the component of following mass percent: golden yellow vertical chain
Mold fermentation object 10%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 10%, Wuyi bacterium conidia powder
10%, bacillus mycelia 10%, choline chloride 0.5%, light rare earth 0.5%, vitamins 1%, microelement 1%, wetting
Agent 3%, dispersing agent 3%, stabilizer 3%, filler are supplied to 100%.
The vitamins is the mixture of vitamin B and C, and the mass ratio of the two is 1:1:1；The microelement be B,
Mn, Mo, Cu, Zn and Fe, wherein the mass ratio of B, Mn, Mo, Cu, Zn and Fe are 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe
From following raw material: borax, manganese sulfate, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent be Tween-80 and
Mixture in sodium methylene bis-naphthalene sulfonate, wherein the mass ratio of the two is 1:1；Dispersing agent is calcium lignosulfonate；Stabilizer
For vitamin E；The filler is the mixture of kaolin and diatomite, wherein the mass ratio of the two is 1:2.
The complex microorganism Wettable pesticide powder of the present embodiment is prepared using preparation method as described in Example 1.
The release rate of the complex microorganism Wettable pesticide powder of the present invention of embodiment 5 detects
Complex microorganism Wettable pesticide powder described in 1-4 of the embodiment of the present invention is placed in and is stored at room temperature, is claimed after 2d
Sample is added in the sterile water with bead, and by gradient dilution, coating detects the various viable bacteria bodies discharged in pesticide sample
Quantity is repeated 3 times, wherein by bacillus, rose yellow streptomycete, actinomyces styreptomyces globispotus strain release rate for, three
Release rate calculation method be respectively as follows: release rate (%)=release bacillus number (cfu)/inoculation bacillus number (cfu) ×
100%, release rate (%)=release rose yellow streptomycete number (cfu)/inoculation rose yellow streptomycete number (cfu) × 100%, release
Rate (%)=release actinomyces styreptomyces globispotus strain number (cfu)/inoculation actinomyces styreptomyces globispotus strain number (cfu) × 100%, detection knot
Fruit is as shown in the table:
The release rate of 1 bacillus of table
The release rate of 2 rose yellow streptomycete of table
The release rate of 3 actinomyces styreptomyces globispotus strain of table
As can be seen that the complex microorganism Wettable pesticide powder of 1-4 of embodiment of the present invention preparation from the data of table 1-3
Microbiological release rate it is high, and using being formulated the micro- of complex microorganism Wettable pesticide powder obtained described in embodiment 4
Biogenic emission rate highest, using the Microbiological release of the complex microorganism Wettable pesticide powder obtained of formula described in embodiment 3
Rate is taken second place.
The different fillers of embodiment 6, wetting agent, dispersing agent, stabilizer are to complex microorganism wettable powder agriculture of the invention
The Index Influence of medicine
A kind of complex microorganism Wettable pesticide powder is prepared first, in accordance with method described in embodiment 1, wherein in addition to filling out
The substance classes of material are different outer, remaining ingredient and content are all the same, then measure its suspensibility (the People's Republic of China country mark
Quasi- GB/T 14825-2006), wetting time (the People's Republic of China's standard GB/T/T 5451-2001) and viable count (adopt
Bacterium colony counting is carried out with serial dilutions method), as a result as shown in the table:
Index Influence of the different fillers of table 4 to complex microorganism Wettable pesticide powder of the invention
Known by table 4, filler be kaolin and diatomite mixture, and the mass ratio of the two be 1:2 when, gained pesticide
Suspensibility is best, wetting time is most short, number of viable is most, and followed by filler is bentonite and kaolinic mixture, quality
When than for 1:1, the suspensibility of gained pesticide is preferably, wetting time is shorter, number of viable is more.Next, we fix filler
For the mixture of kaolin and diatomite, and the mass ratio of the two is 1:2, is tested the variety classes of wetting agent, as a result
It is shown in Table 2:
Index Influence of the 5 different wetting agent of table to complex microorganism Wettable pesticide powder of the invention
As shown in Table 5, generally, complex microorganism Wettable pesticide powder of the present invention by lauryl sodium sulfate, spit
Temperature -80, sodium methylene bis-naphthalene sulfonate are all good selection as wetting agent, but wetting agent is Tween-80 and di-2-ethylhexylphosphine oxide
Mixture in sodium naphthalene sulfonate, and the mass ratio of the two be 1:1 when, wetability is best, followed by sodium methylene bis-naphthalene sulfonate, spits
The wetability effect of temperature -80 is worst.Next, we fix the mixture that filler is kaolin and diatomite, and the matter of the two
Amount ratio is 1:2, fixed wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, and the mass ratio of the two is 1:
1, the variety classes of dispersing agent are tested, the results are shown in Table 6:
Index Influence of the different dispersing agents of table 6 to complex microorganism Wettable pesticide powder of the invention
| Dispersing agent | Dispersibility | Viable count (× 109cfu/g) |
| Sodium lignin sulfonate | 5.6 | |
| Calcium lignosulfonate | ++ | 5.9 |
| Poly-aspartic-acid | + | 5.7 |
Note: dispersibility indicates that by force good dispersion is indicated with "+" with " ++ ", and dispersibility is generally not done
As can be seen from Table 6, the dispersibility of calcium lignosulfonate is best, followed by poly-aspartic-acid, lignin sulfonic acid
Sodium is general as the effect of dispersibility.Next, we fix the mixture that filler is kaolin and diatomite, and the matter of the two
Amount ratio is 1:2, fixed wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, and the mass ratio of the two is 1:
1, fixed dispersing agent is calcium lignosulfonate, tests the variety classes of stabilizer, the results are shown in Table 7,
Index Influence of 7 different stabilizers of table to complex microorganism Wettable pesticide powder of the invention
As shown in Table 7, vitamin E is best as the effect of stabilizer, and number of viable is most, and potassium dihydrogen phosphate is as stabilization
The effect of agent is not so good as vitamin E.It can be seen from the above result that mixture of the filler for kaolin and diatomite, and the quality of the two
Than for 1:2, wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, and the mass ratio of the two is 1:1, dispersion
Agent is calcium lignosulfonate, and when stabilizer is vitamin E, obtained complex microorganism Wettable pesticide powder of the invention has
Optimal effect.
The effect experiment of curing the disease of the complex microorganism Wettable pesticide powder of the invention of embodiment 7
According to the complex microorganism Wettable pesticide powder of formula preparation comparative example as described in example 4, with embodiment 4
Difference be only that the microbiology class in the complex microorganism Wettable pesticide powder of comparative example only contains golden yellow vertical strepto-
Bacterium fermentation material, and do not contain rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder and bacillus
Mycelia, the ingredient lacked use filler polishing.
Next, answering using complex microorganism Wettable pesticide powder described in 1-4 of the embodiment of the present invention and comparative example
Conjunction Microbial wettable powder pesticide, commercially available carbendazim are used for the prevention and treatment of banana blight together, such as more according to conventional suspension liquid
The application method and dosage of bacterium spirit, as a result see the table below:
The complex microorganism wettable powder agriculture of the complex microorganism Wettable pesticide powder of the invention of table 8 and comparative example
Medicine, carbendazim are to the control efficiency comparative experiments of banana blight
| Pesticide | Scab inhibiting rate | Integrated control effect |
| Embodiment 1 | 80% | 81% |
| Embodiment 2 | 82% | 82% |
| Embodiment 3 | 85% | 84% |
| Embodiment 4 | 88% | 87% |
| Comparative example | 72% | 74% |
| Carbendazim | 70% | 73% |
Complex microorganism Wettable pesticide powder of the invention is with carbendazim, only containing golden yellow vertical as can be seen from the above table
Streptomycete fermentation object and do not contain rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder and gemma
The Microbial wettable powder pesticide of bacillus mycelia is compared, and complex microorganism Wettable pesticide powder of the invention can be better
For preventing and treating banana blight, and the complex microorganism Wettable pesticide powder control efficiency of embodiment 4 is best, and synthesis is anti-
It controls effect and is up to 80%, the scab inhibiting rate and integrated control effect of embodiment 3 are only second to embodiment 4.
Next, according to the complex microorganism Wettable pesticide powder of formula preparation comparative example as described in example 4, with
The difference of embodiment 4 is only that the microbiology class in the complex microorganism Wettable pesticide powder of comparative example only contains Wuyi
Bacterium conidia powder, and without containing golden yellow vertical streptomycete fermentation object, rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder and
Bacillus mycelia, the ingredient lacked use filler polishing.
Then, using the compound of complex microorganism Wettable pesticide powder described in 1-4 of the embodiment of the present invention and comparative example
Microbial wettable powder pesticide, commercially available carbendazim are used for the prevention and treatment of winter jujube rust together, according to conventional suspension liquid such as carbendazim
Application method and dosage, as a result see the table below:
The complex microorganism wettable powder agriculture of the complex microorganism Wettable pesticide powder of the invention of table 9 and comparative example
Medicine, carbendazim are to the control efficiency comparative experiments of winter jujube rust
| Pesticide | Scab inhibiting rate | Integrated control effect |
| Embodiment 1 | 82% | 82% |
| Embodiment 2 | 83% | 84% |
| Embodiment 3 | 85% | 86% |
| Embodiment 4 | 88% | 89% |
| Comparative example | 72% | 74% |
| Carbendazim | 68% | 72% |
As can be seen from the above table complex microorganism Wettable pesticide powder of the invention and carbendazim, only contain Wuyi bacterium spore
Sub- powder and do not contain golden yellow vertical streptomycete fermentation object, rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder and gemma
The Microbial wettable powder pesticide of bacillus mycelia is compared, and complex microorganism Wettable pesticide powder of the invention can be better
For preventing and treating winter jujube rust, and the complex microorganism Wettable pesticide powder control efficiency of embodiment 4 is best, integrated control
Effect is up to 80%, and the scab inhibiting rate and integrated control effect of embodiment 3 are only second to embodiment 4.Next, according to embodiment
The complex microorganism Wettable pesticide powder of formula preparation comparative example described in 4, the difference with embodiment 4 is only that, is compared
Microbiology class in the complex microorganism Wettable pesticide powder of example only contains rose yellow streptomycete mycelia, and without containing golden yellow
Vertical streptomycete fermentation object, Wuyi bacterium conidia powder, actinomyces styreptomyces globispotus strain original powder and bacillus mycelia, the ingredient lacked are adopted
With filler polishing.
Then, using the compound of complex microorganism Wettable pesticide powder described in 1-4 of the embodiment of the present invention and comparative example
Microbial wettable powder pesticide, commercially available carbendazim are used for the prevention and treatment of cotton verticillium wilt together, according to for example more bacterium of conventional suspension liquid
The application method and dosage of spirit, as a result see the table below:
The complex microorganism wettable powder agriculture of the complex microorganism Wettable pesticide powder of the invention of table 10 and comparative example
Medicine, carbendazim are to the control efficiency comparative experiments of cotton verticillium wilt
| Pesticide | Scab inhibiting rate | Integrated control effect |
| Embodiment 1 | 83% | 82% |
| Embodiment 2 | 84% | 82% |
| Embodiment 3 | 86% | 85% |
| Embodiment 4 | 89% | 88% |
| Comparative example | 72% | 74% |
| Carbendazim | 68% | 72% |
As can be seen from the above table complex microorganism Wettable pesticide powder of the invention and carbendazim, only contain rose yellow chain
Fungal hyphae and do not contain golden yellow vertical streptomycete fermentation object, Wuyi bacterium conidia powder, actinomyces styreptomyces globispotus strain original powder and gemma
The Microbial wettable powder pesticide of bacillus mycelia is compared, and complex microorganism Wettable pesticide powder of the invention can be better
For preventing and treating cotton verticillium wilt, and the complex microorganism Wettable pesticide powder control efficiency of embodiment 4 is best, and synthesis is anti-
It controls effect and is up to 86%, the scab inhibiting rate and integrated control effect of embodiment 3 are only second to embodiment 4.
In addition to this, present inventor also found complex microorganism Wettable pesticide powder of the invention in addition to having very
Except good preventing disease theraping effect, also have effects that promote crop yield.
It is as a result it has also been found that of the invention using complex microorganism Wettable pesticide powder prevention peanut dead leaf disease of the invention
Complex microorganism Wettable pesticide powder can also be greatly facilitated peanut yield increasing, result other than it can prevent and treat peanut dead leaf disease
It see the table below:
The complex microorganism Wettable pesticide powder of the invention of table 11 and carbendazim test the effect of increasing production of peanut
Complex microorganism Wettable pesticide powder of the invention is obvious to peanut yield increasing as can be seen from the above table, wherein implementing
Complex microorganism Wettable pesticide powder described in example 4 is most obvious to peanut yield increasing effect, complex microorganism described in embodiment 3
Wettable pesticide powder takes second place to peanut yield increasing effect.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of range is protected, although the invention is described in detail with reference to the preferred embodiments, those skilled in the art should
Understand, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the essence of technical solution of the present invention
And range.
Claims (9)
1. a kind of complex microorganism Wettable pesticide powder, which is characterized in that it is made of the component of following mass percent: gold
Yellow vertical streptomycete fermentation object 10%~15%, rose yellow streptomycete mycelia 10%~15%, actinomyces styreptomyces globispotus strain original powder
10%~15%, Wuyi bacterium conidia powder 10%~15%, bacillus mycelia 10%~15%, choline chloride 0.5%~2%,
Light rare earth 0.5%~2%, vitamins 0.5%~2%, microelement 0.5%~2%, wetting agent 2%~5%, dispersing agent
2%~5%, stabilizer 2%~5%, filler are supplied to 100%；
The vitamins is the mixture of vitamin B and C, and the mass ratio of the two is 1:1, the wetting agent be Tween-80 and
Mixture in sodium methylene bis-naphthalene sulfonate, wherein the mass ratio of the two is 1:1；The dispersing agent is calcium lignosulfonate；Institute
Stating stabilizer is vitamin E；The filler is the mixture of kaolin and diatomite, wherein the mass ratio of the two is 1:2.
2. a kind of complex microorganism Wettable pesticide powder according to claim 1, which is characterized in that described golden yellow vertical
Streptomycete fermentation object is prepared by following steps: 1) activating golden yellow vertical streptomycete: being connect golden yellow vertical streptomycete with method of scoring
Kind on Gause I culture medium flat plate, 28 DEG C constant temperature incubation 4 days, the mass percentage composition of culture medium: soluble starch
2.5%, KNO30.2%, K2HPO40.05%, MgSO40.05%, NaCl 0.05%, FeSO40.05%, agar 2.5%,
Moisturizing is to 100%；2) the golden yellow vertical streptomycete of activation is inoculated in first order seed culture solution, 180rmin-1, 28 DEG C of trainings
Support 72h, the mass percentage composition of first order seed culture solution: soluble starch 2.5%, KNO30.2%, K2HPO40.05%,
MgSO40.05%, NaCl 0.05%, FeSO40.05%, moisturizing to 100%；3) the first order seed culture solution prepared is connect
Kind is in secondary seed culture solution, 180rmin-1, 28 DEG C of culture 72h, centrifuge separation, obtain golden yellow vertical streptomycete fermentation
Object, low temperature drying, moisture content are lower than 5%, the mass percentage composition of secondary seed culture solution: glucose 0.05%, solvable
Property starch 0.05%, K2HPO40.05%, NaCl 0.05%, MgSO40.05%, peptone 0.05%, 0.05% and of yeast extract
Soybean powder 0.05%, moisturizing to 100%；
The rose yellow streptomycete mycelia is prepared by following steps: 1) by rose yellow streptomycete in 28 DEG C of trainings in PDA culture medium
It supports 5 days；2) it is then inoculated into seed culture medium with 2% ratio, condition of culture are as follows: 28 DEG C, 180rmin-1, it cultivates 3 days,
Wherein seed culture medium are as follows: 3.5% groundnut meal, 5% starch, 1% glucose, 0.8% sodium chloride, 0.3% alcohol mother's cream,
0.8% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.4% calcium carbonate, 0.2% ferrous sulfate, 0.2% zinc sulfate, 3.5% sucrose,
0.4% fructose；3) the rose yellow streptomycete culture that step (2) is prepared is by being obtained by filtration the rose yellow streptomycete
Mycelia；
The actinomyces styreptomyces globispotus strain original powder pass through following steps prepare: 1) by activated actinomyces styreptomyces globispotus strain with
2% ratio, which is inoculated into, is inoculated in Gause I fluid nutrient medium, and 28 DEG C, 150-160rmin-1It is obtained after shaken cultivation 120h
Actinomyces seed culture fluid；2) the actinomyces styreptomyces globispotus strain that step (2) is prepared passed through into filtering, be dried to obtain described put
Line bacterium styreptomyces globispotus strain original powder；
The bacillus mycelia is prepared by following steps: activated bacillus is inoculated in training in 2% ratio
It supports in base, is cultivated 5 days at 28 DEG C, take out culture and be filtered, obtained solid is bacillus mycelia；It is wherein described
Bacillus is bacillus licheniformis, bacillus subtilis or wax bacillus.
3. a kind of complex microorganism Wettable pesticide powder according to claim 1, which is characterized in that it is by following quality
The component of percentage is made: golden yellow vertical streptomycete fermentation object 10%~12%, is put at rose yellow streptomycete mycelia 10%~12%
Line bacterium styreptomyces globispotus strain original powder 10%~12%, Wuyi bacterium conidia powder 10%~12%, bacillus mycelia 10%~12%, chlorine
Change choline 0.5%~1%, light rare earth 0.5%~1%, vitamins 0.5%~1%, microelement 0.5%~1%, wetting
Agent 2%~4%, dispersing agent 2%~4%, stabilizer 2%~4%, filler are supplied to 100%.
4. a kind of complex microorganism Wettable pesticide powder according to claim 1, which is characterized in that it is by following quality
The component of percentage is made: golden yellow vertical streptomycete fermentation object 10%, rose yellow streptomycete mycelia 10%, actinomyces ball spore strepto-
Bacterium original powder 10%, Wuyi bacterium conidia powder 10%, bacillus mycelia 10%, choline chloride 0.5%, light rare earth 0.5%, vitamin
Class 1%, microelement 1%, wetting agent 3%, dispersing agent 3%, stabilizer 3%, filler are supplied to 100%.
5. a kind of complex microorganism Wettable pesticide powder according to claim 1, which is characterized in that the vitamins
For at least one of vitamin B or C；The microelement be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo, Cu, Zn and
The mass ratio of Fe be 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, manganese sulfate, sodium molybdate,
Copper sulphate, zinc sulfate, ferrous sulfate；The wetting agent is lauryl sodium sulfate, in Tween-80, sodium methylene bis-naphthalene sulfonate
At least one；The dispersing agent is at least one of sodium lignin sulfonate, sodium lignin sulfonate calcium, poly-aspartic-acid；
The stabilizer is at least one of vitamin E, potassium dihydrogen phosphate；The filler is diatomite, in bentonite, kaolin
It is at least one.
6. a kind of complex microorganism Wettable pesticide powder according to claim 1, which is characterized in that it is by following quality
The component of percentage is made: golden yellow vertical streptomycete fermentation object 15%, rose yellow streptomycete mycelia 12%, actinomyces ball spore strepto-
Bacterium original powder 15%, Wuyi bacterium conidia powder 12%, bacillus mycelia 15%, choline chloride 1%, light rare earth 2%, vitamins
2%, microelement 1%, wetting agent 4%, dispersing agent 4%, stabilizer 4%, filler are supplied to 100%；Wherein vitamins is
The mixture of vitamin B and C, the mass ratio of the two are 1:1；Microelement be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo,
The mass ratio of Cu, Zn and Fe be 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, manganese sulfate,
Sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is sodium methylene bis-naphthalene sulfonate；Dispersing agent is poly-aspartic-acid；
Stabilizer is the mixture in vitamin E and potassium dihydrogen phosphate, wherein the mass ratio of the two is 1:1；Filler is bentonite and height
The mixture of ridge soil, wherein the mass ratio of the two is 1:1.
7. a kind of complex microorganism Wettable pesticide powder according to claim 1, which is characterized in that it is by following quality
The component of percentage is made: golden yellow vertical streptomycete fermentation object 10%, rose yellow streptomycete mycelia 10%, actinomyces ball spore strepto-
Bacterium original powder 10%, Wuyi bacterium conidia powder 10%, bacillus mycelia 10%, choline chloride 0.5%, light rare earth 0.5%, vitamin
Class 1%, microelement 1%, wetting agent 3%, dispersing agent 3%, stabilizer 3%, filler are supplied to 100%；The wherein dimension life
Plain class is the mixture of vitamin B and C, and the mass ratio of the two is 1:1:1；The microelement be B, Mn, Mo, Cu, Zn and Fe,
Wherein the mass ratio of B, Mn, Mo, Cu, Zn and Fe are 4:5:2:5:2:7, and B, Mn, Mo, Cu, Zn and Fe are from following raw material:
Borax, manganese sulfate, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is in Tween-80 and sodium methylene bis-naphthalene sulfonate
Mixture, wherein both mass ratio be 1:1；Dispersing agent is calcium lignosulfonate；Stabilizer is vitamin E；The filler
For the mixture of kaolin and diatomite, wherein both mass ratio be 1:2.
8. a kind of preparation method of complex microorganism Wettable pesticide powder as described in any one of claims 1 to 7, feature
It is, the described method comprises the following steps: according to the ratio by wetting agent, dispersing agent, stabilizer, filler, choline chloride, light
Rare earth is put into airslide disintegrating mill and is crushed, and 350 meshes are crossed, and after cooling, golden yellow vertical streptomycete fermentation object, rose yellow chain is added
Fungal hyphae, actinomyces styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder, bacillus mycelia, vitamins, microelement, after
Continued powder is broken, obtains the complex microorganism Wettable pesticide powder.
9. the described in any item complex microorganism Wettable pesticide powders of claim 1~7 are in prevention and treatment banana blight, winter jujube rust
Purposes in disease, cotton verticillium wilt and peanut yield increasing.
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| CN102206593A (en) * | 2011-03-15 | 2011-10-05 | 海南大学 | Streptomyces aureoverticillatus strain and its application |
| CN102381912A (en) * | 2011-11-09 | 2012-03-21 | 潍坊万胜生物农药有限公司 | Microbial pesticide preparation for plant protection and growth regulation |
| CN103772037A (en) * | 2014-01-05 | 2014-05-07 | 石达璘 | Continuous cropping resistant high-yield agent for fruits and vegetables |
| CN104686582A (en) * | 2013-12-06 | 2015-06-10 | 大连三科生物工程有限公司 | Preparation method of microorganism bacterium agent for resisting potato scab |
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|---|---|---|---|---|
| CN102206593A (en) * | 2011-03-15 | 2011-10-05 | 海南大学 | Streptomyces aureoverticillatus strain and its application |
| CN102381912A (en) * | 2011-11-09 | 2012-03-21 | 潍坊万胜生物农药有限公司 | Microbial pesticide preparation for plant protection and growth regulation |
| CN104686582A (en) * | 2013-12-06 | 2015-06-10 | 大连三科生物工程有限公司 | Preparation method of microorganism bacterium agent for resisting potato scab |
| CN103772037A (en) * | 2014-01-05 | 2014-05-07 | 石达璘 | Continuous cropping resistant high-yield agent for fruits and vegetables |
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