CN106804627A - A kind of complex microorganism Wettable pesticide powder and preparation method thereof and purposes - Google Patents
A kind of complex microorganism Wettable pesticide powder and preparation method thereof and purposes Download PDFInfo
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- CN106804627A CN106804627A CN201710067939.3A CN201710067939A CN106804627A CN 106804627 A CN106804627 A CN 106804627A CN 201710067939 A CN201710067939 A CN 201710067939A CN 106804627 A CN106804627 A CN 106804627A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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Abstract
The invention discloses a kind of complex microorganism Wettable pesticide powder, it is made up of the component of following mass percent：Golden yellow vertical streptomycete fermentation thing 10%~15%, rose yellow streptomycete mycelia 10%~15%, actinomyces styreptomyces globispotus strain original powder 10%~15%, Wuyi bacterium conidia powder 10%~15%, bacillus mycelia 10%~15%, Choline Chloride 0.5%~2%, light rare earth 0.5%~2%, vitamins 0.5%~2%, trace element 0.5%~2%, wetting agent 2%~5%, dispersant 2%~5%, stabilizer 2%~5%, filler are supplied to 100%.Complex microorganism Wettable pesticide powder composition collocation science of the invention, compatibility is reasonable；Has a broad antifungal spectrum, activity is high, all has good preventing disease theraping effect to many crop diseases, effectively reduces cost accounting；Environmental pollution is small, there is nuisanceless and the characteristics of without residual hazard, effectively prevent the harm of chemical pesticide.In addition, the invention discloses the Preparation method and use of the complex microorganism Wettable pesticide powder.
Description
Technical field
The present invention relates to a kind of complex microorganism Wettable pesticide powder and preparation method thereof and purposes, belong to agricultural chemicals neck
Domain.
Background technology
Domestic at present for plant, especially the preventing and treating of corps diseases is substantially and uses chemical pesticide, in chemical pesticide
In the case that drawback is increasingly highlighted, some microbial pesticides gradually receive the highest attention of domestic and foreign scholars.Microbial source
The exploitation and application of agricultural chemicals have been increasingly becoming the new trend of pesticide industry, and its main advantage is：1) species is various, the choosing of research and development
Select leeway big；2) selectivity is strong, to non-target organism safety, is not likely to produce the resistance to the action of a drug；3) activity is high, low production cost, fermentation
Process is simple；4) it is environmentally safe, nuisanceless, noresidue.
But the agricultural chemicals of existing microbial source can be only applied to a kind of prevention and control of or class disease, and its application limitation is very
Greatly, peasant also is intended to a kind of agricultural chemicals and can be only applied to one rather than a kind of agricultural chemicals with as much as possible suitable for various diseases
Plant or a class disease, therefore, develop a kind of safety, low toxicity, stabilization, the efficient, microbial pesticide of has a broad antifungal spectrum and compel in eyebrow
Eyelash.
The content of the invention
It is an object of the invention to develop a kind of complex microorganism Wettable pesticide powder, the microbial pesticide release
Rate is high, has a broad antifungal spectrum, can efficiently prevent and cure diseases, and safety, low toxicity, containing various nutriments, beneficial to crop yield, can grow
Phase stabilization is preserved.
To achieve the above object, the technical scheme taken of the present invention is：A kind of complex microorganism Wettable pesticide powder, its
It is made up of the component of following mass percent：Golden yellow vertical streptomycete fermentation thing 10%~15%, rose yellow streptomycete mycelia
10%~15%, actinomyces styreptomyces globispotus strain original powder 10%~15%, Wuyi bacterium conidia powder 10%~15%, bacillus mycelia
10%~15%, Choline Chloride 0.5%~2%, light rare earth 0.5%~2%, vitamins 0.5%~2%, trace element
0.5%~2%, wetting agent 2%~5%, dispersant 2%~5%, stabilizer 2%~5%, filler are supplied to 100%.
Preferably, the preserving number of golden yellow vertical streptomycete is CCTCC NO.M2010293, and the preservation of rose yellow streptomycete is compiled
Number it is CGMCC NO.1471, the deposit number of actinomyces styreptomyces globispotus strain is CCTCC NO.M 2011012, and bacillus is ground
Clothing bacillus, bacillus subtilis or wax bacillus.
Wherein Choline Chloride, light rare earth, vitamin etc. are conducive to plant stress-resistance growth promoting, with obvious disease resistance, resist
Effect that is inverse, improving crop yield, improve crop products quality, the Microbiological release in microbial pesticide under this formula
Rate is high, has a broad antifungal spectrum, and safe and environment-friendly.
Preferably, the golden yellow vertical streptomycete fermentation thing is prepared by following steps：1) golden yellow vertical streptomycete is activated：
Golden yellow vertical streptomycete is seeded on Gause I culture medium flat plate with method of scoring, 28 DEG C incubated 4 days, the matter of culture medium
Amount percentage composition：Soluble starch 2.5%, KNO30.2%th, K2HPO40.05%th, MgSO40.05%th, NaCl 0.05%,
FeSO40.05%, agar 2.5%, moisturizing to 100%；2) the golden yellow vertical streptomycete for activating is inoculated in first order seed culture
In liquid, 180rmin-1, 28 DEG C of culture 72h, the mass percent composition of first order seed nutrient solution：Soluble starch 2.5%,
KNO30.2%, K2HPO40.05%th, MgSO40.05%th, NaCl 0.05%, FeSO40.05%, moisturizing to 100%；3) will prepare
Good first order seed nutrient solution is seeded in secondary seed nutrient solution, 180rmin-1, 28 DEG C are cultivated 72h, and centrifugation is obtained
Golden yellow vertical streptomycete fermentation thing, low temperature drying, moisture is less than 5%, and the mass percent of secondary seed nutrient solution is constituted：
Glucose 0.05%, soluble starch 0.05%, K2HPO40.05%th, NaCl 0.05%, MgSO40.05%th, peptone
0.05%th, yeast extract 0.05% and analysis for soybean powder 0.05%, moisturizing to 100%.
Preferably, the rose yellow streptomycete mycelia is prepared by following steps：1) rose yellow streptomycete is cultivated in PDA
Cultivated 5 days for 28 DEG C on base；2) and then with 2% ratio it is inoculated into seed culture medium, condition of culture is：28℃、180r·
min-1, cultivate 3 days, wherein seed culture medium is：3.5% groundnut meal, 5% starch, 1% glucose, 0.8% sodium chloride,
0.3% alcohol mother's cream, 0.8% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.4% calcium carbonate, 0.2% ferrous sulfate, 0.2% sulfuric acid
Zinc, 3.5% sucrose, 0.4% fructose；3) the rose yellow streptomycete culture for preparing step (2) is by being filtrated to get
State rose yellow streptomycete mycelia.
Preferably, the actinomyces styreptomyces globispotus strain original powder is prepared by following steps：1) actinomyces that will have been activated
Styreptomyces globispotus strain is inoculated into 2% ratio and is inoculated in Gause I fluid nutrient medium, 28 DEG C, 150-160rmin-1Vibration
Actinomyces seed culture fluid is obtained after culture 120h；2) the actinomyces styreptomyces globispotus strain for preparing step (2) by filtering,
It is dried to obtain the actinomyces styreptomyces globispotus strain original powder.
Preferably, the bacillus mycelia is prepared by following steps：The bacillus that will have been activated by 2% ratio
Example is inoculated in culture medium, is cultivated 5 days at 28 DEG C, is taken out culture and is filtered, the solid for obtaining as bacillus bacterium
Silk；Wherein described bacillus is bacillus licheniformis, bacillus subtilis or wax bacillus.
Preferably, the complex microorganism Wettable pesticide powder is made up of the component of following mass percent：It is golden yellow to hang down
Straight chain mold fermentation thing 10%~12%, rose yellow streptomycete mycelia 10%~12%, actinomyces styreptomyces globispotus strain original powder 10%
~12%, Wuyi bacterium conidia powder 10%~12%, bacillus mycelia 10%~12%, Choline Chloride 0.5%~1%, light dilute
Soil 0.5%~1%, vitamins 0.5%~1%, trace element 0.5%~1%, wetting agent 2%~4%, dispersant 2%~
4%th, stabilizer 2%~4%, filler is supplied to 100%.This formula under agricultural chemicals wetability, dispersiveness, suspension, granulating rate,
Stability is higher, and preventing disease theraping effect is preferable.
It is furthermore preferred that the complex microorganism Wettable pesticide powder is made up of the component of following mass percent：It is golden yellow
Vertical streptomycete fermentation thing 10%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 10%, Wuyi bacterium spore
Powder 10%, bacillus mycelia 10%, Choline Chloride 0.5%, light rare earth 0.5%, vitamins 1%, trace element 1%, profit
Humectant 3%, dispersant 3%, stabilizer 3%, filler are supplied to 100%.The lower complex microorganism Wettable pesticide powder of this formula
Wetability, dispersiveness, suspension, granulating rate, stability be all up optimum level, after measured, complex microorganism wettable powder
Can be deposited 6 months under the shelf storage life normal temperature of agent agricultural chemicals, bacteriostasis rate is positively retained at more than 85%, and preventing disease theraping effect is most
It is excellent.
Preferably, the vitamins is at least one in vitamin, B or C.It is furthermore preferred that the vitamins is
The mixture of vitamin B and C, both mass ratioes are 1：1.
Preferably, the trace element is B, Mn, Mo, Cu, Zn and Fe.Preferred described B, Mn, Mo, Cu, Zn and Fe
Mass ratio be 4：5：2：5：2：7, B, Mn, Mo, Cu, Zn and Fe come from following raw material：Borax, manganese sulfate, sodium molybdate, sulphur
Sour copper, zinc sulfate, ferrous sulfate.When using the quality proportioning it is micro- when, the complex microorganism wettable powder
Agricultural chemicals is performed at utmost to the production-increasing function of crops.
Preferably, the wetting agent is at least in lauryl sodium sulfate, Tween-80, sodium methylene bis-naphthalene sulfonate
Kind；The dispersant is at least one in sodium lignin sulfonate, sodium lignin sulfonate calcium, poly-aspartic-acid；The stabilization
Agent is at least one in vitamin E, potassium dihydrogen phosphate；Described filler is at least in diatomite, bentonite, kaolin
Kind.
It is furthermore preferred that the wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, wherein both matter
Amount is than being 1:1；The dispersant is sodium lignin sulfonate calcium；The stabilizer is vitamin E；Described filler is kaolin and silicon
The mixture of diatomaceous earth, wherein both mass ratioes are 1:2.
Preferably, a kind of complex microorganism Wettable pesticide powder of the present invention, its by following mass percent group
Divide and be made：Golden yellow vertical streptomycete fermentation thing 15%, rose yellow streptomycete mycelia 12%, actinomyces styreptomyces globispotus strain original powder
15%th, Wuyi bacterium conidia powder 12%, bacillus mycelia 15%, Choline Chloride 1%, light rare earth 2%, vitamins 2%, micro
Element 1%, wetting agent 4%, dispersant 4%, stabilizer 4%, filler are supplied to 100%；Wherein vitamins be vitamin B and
The mixture of C, both mass ratioes are 1：1；Trace element is B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo, Cu, Zn and Fe
Mass ratio be 4：5：2：5：2：7, B, Mn, Mo, Cu, Zn and Fe come from following raw material：Borax, manganese sulfate, sodium molybdate, sulphur
Sour copper, zinc sulfate, ferrous sulfate；Wetting agent is sodium methylene bis-naphthalene sulfonate；Dispersant is poly-aspartic-acid；Stabilizer is dimension
Mixture in raw element E and potassium dihydrogen phosphate, wherein both mass ratioes are 1:1；Filler is bentonite and kaolinic mixing
Thing, wherein both mass ratioes are 1:1.
It is furthermore preferred that component system of the complex microorganism Wettable pesticide powder of the present invention by following mass percent
Into：Golden yellow vertical streptomycete fermentation thing 10%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 10%, force
Smooth bacterium conidia powder 10%, bacillus mycelia 10%, Choline Chloride 0.5%, light rare earth 0.5%, vitamins 1%, micro unit
Element 1%, wetting agent 3%, dispersant 3%, stabilizer 3%, filler are supplied to 100%；Wherein described vitamins is vitamin B
With the mixture of C, both mass ratioes are 1：1：1；It is described trace element be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo,
The mass ratio of Cu, Zn and Fe is 4：5：2：5：2：7, B, Mn, Mo, Cu, Zn and Fe come from following raw material：Borax, manganese sulfate,
Sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, its
In both mass ratio be 1:1；Dispersant is calcium lignosulfonate；Stabilizer is vitamin E；Described filler is kaolin and silicon
The mixture of diatomaceous earth, wherein both mass ratioes are 1:2.
In addition, the present invention discloses a kind of preparation method of complex microorganism Wettable pesticide powder described above, its bag
Include following steps：Wetting agent, dispersant, stabilizer, filler, Choline Chloride, light rare earth are put into air-flow according to the ratio
Crushed in pulverizer, cross 350 mesh sieves, after cooling, add golden yellow vertical streptomycete fermentation thing, rose yellow streptomycete mycelia, unwrapping wire
Bacterium styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder, bacillus mycelia, vitamins, trace element, continue to crush, and obtain institute
State complex microorganism Wettable pesticide powder.
In addition, the present invention discloses a kind of complex microorganism Wettable pesticide powder described above being withered in preventing and treating banana
Purposes in disease, winter jujube rust, cotton verticillium wilt and peanut yield increasing.
The beneficial effects of the present invention are：Complex microorganism Wettable pesticide powder composition collocation science of the invention, matches somebody with somebody
5 is reasonable；Has a broad antifungal spectrum, activity is high, all has good preventing disease theraping effect to many crop diseases, effectively reduces and prevents and treats into
This；Production cost is relatively low, favorably improves the competitiveness of product in market；Environmental pollution is small, there is spy nuisanceless and without residual hazard
Point, effectively prevent the harm of chemical pesticide.
Specific embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of complex microorganism Wettable pesticide powder, it is made up of the component of following mass percent：Golden yellow vertical chain
Mold fermentation thing 10%, rose yellow streptomycete mycelia 15%, actinomyces styreptomyces globispotus strain original powder 10%, Wuyi bacterium conidia powder
15%th, bacillus mycelia 10%, Choline Chloride 2%, light rare earth 0.5%, vitamins 0.5%, trace element 2%, wetting
Agent 2%, dispersant 5%, stabilizer 5%, filler are supplied to 100%.
Wherein, the vitamins is vitamin C；It is described trace element be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn,
The mass ratio of Mo, Cu, Zn and Fe is 4：5：2：5：2：7, B, Mn, Mo, Cu, Zn and Fe come from following raw material：Borax, sulfuric acid
Manganese, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is lauryl sodium sulfate；The dispersant is sulfomethylated lignin
Sour sodium；The stabilizer is vitamin E；Described filler is diatomite.
The complex microorganism Wettable pesticide powder of the present embodiment is prepared from using following methods：According to above-mentioned recipe ratio
Example is crushed during wetting agent, dispersant, stabilizer, filler, Choline Chloride, light rare earth are put into airslide disintegrating mill, crosses 350 mesh
Sieve, after cooling, adds golden yellow vertical streptomycete fermentation thing, rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder, Wuyi
Bacterium conidia powder, bacillus mycelia, vitamins, trace element, continue to crush, that is, obtain the complex microorganism wettable powder
Agent agricultural chemicals；
Wherein, golden yellow vertical streptomycete fermentation thing is prepared by following steps：1) golden yellow vertical streptomycete is activated：To rule
Be seeded in golden yellow vertical streptomycete on Gause I culture medium flat plate by method, and 28 DEG C incubated 4 days, the quality percentage of culture medium
Than composition：Soluble starch 2.5%, KNO30.2%th, K2HPO40.05%th, MgSO40.05%th, NaCl 0.05%,
FeSO40.05%, agar 2.5%, moisturizing to 100%；2) the golden yellow vertical streptomycete for activating is inoculated in first order seed culture
In liquid, 180rmin-1, 28 DEG C of culture 72h, the mass percent composition of first order seed nutrient solution：Soluble starch 2.5%,
KNO30.2%, K2HPO40.05%th, MgSO40.05%th, NaCl 0.05%, FeSO40.05%, moisturizing to 100%；3) will prepare
Good first order seed nutrient solution is seeded in secondary seed nutrient solution, 180rmin-1, 28 DEG C are cultivated 72h, and centrifugation is obtained
Golden yellow vertical streptomycete fermentation thing, low temperature drying, moisture is less than 5%, and the mass percent of secondary seed nutrient solution is constituted：
Glucose 0.05%, soluble starch 0.05%, K2HPO40.05%th, NaCl0.05%, MgSO40.05%th, peptone
0.05%th, yeast extract 0.05% and analysis for soybean powder 0.05%, moisturizing to 100%.
Wherein, rose yellow streptomycete mycelia is prepared by following steps：1) by rose yellow streptomycete in PDA culture medium 28
DEG C culture 5 days；2) and then with 2% ratio it is inoculated into seed culture medium, condition of culture is：28℃、180r·min-1, culture
3 days, wherein seed culture medium was：3.5% groundnut meal, 5% starch, 1% glucose, 0.8% sodium chloride, 0.3% alcohol mother's cream,
0.8% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.4% calcium carbonate, 0.2% ferrous sulfate, 0.2% zinc sulfate, 3.5% sucrose,
0.4% fructose；3) the rose yellow streptomycete culture for preparing step (2) is by being filtrated to get the rose yellow streptomycete
Mycelia.
Wherein, actinomyces styreptomyces globispotus strain original powder is prepared by following steps：1) the actinomyces ball spore chain that will have been activated
Mould is inoculated into 2% ratio and is inoculated in Gause I fluid nutrient medium, 28 DEG C, 150-160rmin-1Shaken cultivation 120h
Actinomyces seed culture fluid is obtained afterwards；2) the actinomyces styreptomyces globispotus strain for preparing step (2) is by filtering, being dried to obtain
The actinomyces styreptomyces globispotus strain original powder.
Wherein, bacillus mycelia is prepared by following steps：The bacillus that will have been activated is connect in 2% ratio
Plant in culture medium, cultivated 5 days at 28 DEG C, take out culture and filtered, the solid for obtaining as bacillus mycelia；Its
Described in bacillus be bacillus licheniformis, bacillus subtilis or wax bacillus.
Embodiment 2
A kind of complex microorganism Wettable pesticide powder, it is made up of the component of following mass percent：Golden yellow vertical chain
Mold fermentation thing 12%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 12%, Wuyi bacterium conidia powder
10%th, bacillus mycelia 12%, Choline Chloride 0.5%, light rare earth 1%, vitamins 1%, trace element 0.5%, wetting
Agent 5%, dispersant 2%, stabilizer 2%, filler are supplied to 100%.
The vitamins is vitamin B；It is described trace element be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo, Cu,
The mass ratio of Zn and Fe is 4：5：2：5：2：7, B, Mn, Mo, Cu, Zn and Fe come from following raw material：Borax, manganese sulfate, molybdenum
Sour sodium, copper sulphate, zinc sulfate, ferrous sulfate；Both wetting agent is the mixture of lauryl sodium sulfate and Tween-80, wherein
Mass ratio be 1:2；Dispersant is the mixture of sodium lignin sulfonate and sodium lignin sulfonate calcium, and wherein both mass ratioes are
1:1；Stabilizer is potassium dihydrogen phosphate；Filler is bentonite.
The complex microorganism Wettable pesticide powder of the present embodiment is prepared from using preparation method as described in Example 1.
Embodiment 3
A kind of complex microorganism Wettable pesticide powder, it is made up of the component of following mass percent：Golden yellow vertical chain
Mold fermentation thing 15%, rose yellow streptomycete mycelia 12%, actinomyces styreptomyces globispotus strain original powder 15%, Wuyi bacterium conidia powder
12%th, bacillus mycelia 15%, Choline Chloride 1%, light rare earth 2%, vitamins 2%, trace element 1%, wetting agent
4%th, dispersant 4%, stabilizer 4%, filler are supplied to 100%.
Vitamins is the mixture of vitamin B and C, and both mass ratioes are 1：1；Trace element for B, Mn, Mo, Cu,
The mass ratio of Zn and Fe, wherein B, Mn, Mo, Cu, Zn and Fe is 4：5：2：5：2：7, B, Mn, Mo, Cu, Zn and Fe come from following
Raw material：Borax, manganese sulfate, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is sodium methylene bis-naphthalene sulfonate；Point
Powder is poly-aspartic-acid；Stabilizer is the mixture in vitamin E and potassium dihydrogen phosphate, and wherein both mass ratioes are
1:1；Filler is bentonite and kaolinic mixture, and wherein both mass ratioes are 1:1.The complex microorganism of the present embodiment can
WP agricultural chemicals is prepared from using preparation method as described in Example 1.
Embodiment 4
A kind of complex microorganism Wettable pesticide powder, it is made up of the component of following mass percent：Golden yellow vertical chain
Mold fermentation thing 10%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 10%, Wuyi bacterium conidia powder
10%th, bacillus mycelia 10%, Choline Chloride 0.5%, light rare earth 0.5%, vitamins 1%, trace element 1%, wetting
Agent 3%, dispersant 3%, stabilizer 3%, filler are supplied to 100%.
The vitamins is the mixture of vitamin B and C, and both mass ratioes are 1：1：1；It is described trace element for B,
The mass ratio of Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo, Cu, Zn and Fe is 4：5：2：5：2：7, B, Mn, Mo, Cu, Zn and Fe
Come from following raw material：Borax, manganese sulfate, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent be Tween-80 and
Mixture in sodium methylene bis-naphthalene sulfonate, wherein both mass ratioes are 1:1；Dispersant is calcium lignosulfonate；Stabilizer
It is vitamin E；Described filler is kaolin and diatomaceous mixture, and wherein both mass ratioes are 1:2.
The complex microorganism Wettable pesticide powder of the present embodiment is prepared from using preparation method as described in Example 1.
The release rate detection of the complex microorganism Wettable pesticide powder of the present invention of embodiment 5
Complex microorganism Wettable pesticide powder described in embodiment of the present invention 1-4 is placed in and is stored at room temperature, claimed after 2d
Sample is added in the sterilized water with bead, by gradient dilution, coating, the various viable bacteria bodies discharged in detection pesticide sample
Quantity, is repeated 3 times, wherein by taking bacillus, rose yellow streptomycete, the release rate of actinomyces styreptomyces globispotus strain as an example, its three
Release rate computational methods be respectively：Release rate (%)=release bacillus number (cfu)/inoculation bacillus number (cfu) ×
100%, release rate (%)=release rose yellow streptomycete number (cfu)/inoculation rose yellow streptomycete number (cfu) × 100%, release
Rate (%)=release actinomyces styreptomyces globispotus strain number
(cfu)/inoculation actinomyces styreptomyces globispotus strain number (cfu) × 100%, testing result is as shown in the table：
The release rate of the bacillus of table 1
The release rate of the rose yellow streptomycete of table 2
The release rate of the actinomyces styreptomyces globispotus strain of table 3
As can be seen that the complex microorganism Wettable pesticide powder of embodiment of the present invention 1-4 preparations from the data of table 1-3
Microbiological release rate it is high, and using the micro- of the obtained complex microorganism Wettable pesticide powder of formula described in embodiment 4
Biogenic emission rate highest, using the Microbiological release that obtained complex microorganism Wettable pesticide powder is formulated described in embodiment 3
Rate is taken second place.
The different fillers of embodiment 6, wetting agent, dispersant, stabilizer are to complex microorganism wettable powder agriculture of the invention
The Index Influence of medicine
It is first according to the method described in embodiment 1 and prepares a kind of complex microorganism Wettable pesticide powder, wherein except fills out
The substance classes of material are different outer, remaining composition and content all same, then determine its suspensibility (the People's Republic of China country mark
Quasi- GB/T 14825-2006), wetting time (the People's Republic of China's standard GB/T/T 5451-2001) and viable count (adopt
Colony counting is carried out with serial dilutions method), it is as a result as shown in the table：
Index Influence of the different fillers of table 4 to complex microorganism Wettable pesticide powder of the invention
Known by table 4, filler is kaolin and diatomaceous mixture, and both mass ratioes are 1:When 2, gained agricultural chemicals
Suspensibility is best, wetting time is most short, number of viable most, next to that filler is bentonite and kaolinic mixture, quality
Than being 1:When 1, the suspensibility of gained agricultural chemicals is preferably, wetting time is shorter, number of viable is more.Next, we fix filler
It is kaolin and diatomaceous mixture, and both mass ratioes are 1:2, the variety classes to wetting agent is tested, as a result
It is shown in Table 2：
Index Influence of the different wetting agent of table 5 to complex microorganism Wettable pesticide powder of the invention
As shown in Table 5, generally, complex microorganism Wettable pesticide powder of the present invention by lauryl sodium sulfate, tell
Temperature -80, sodium methylene bis-naphthalene sulfonate is all good selection as wetting agent, but wetting agent is Tween-80 and di-2-ethylhexylphosphine oxide
Mixture in sodium naphthalene sulfonate, and both mass ratioes are 1:When 1, wetability is best, next to that sodium methylene bis-naphthalene sulfonate, tells
The wetability effect of temperature -80 is worst.Next, we fix filler for kaolin and diatomaceous mixture, and both matter
Amount is than being 1:2, fixed wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, and both mass ratioes are 1:
1, the variety classes to dispersant is tested, and the results are shown in Table 6：
Index Influence of the different dispersants of table 6 to complex microorganism Wettable pesticide powder of the invention
| Dispersant | Dispersiveness | |
| Sodium lignin sulfonate | 5.6 | |
| Calcium lignosulfonate | ++ | 5.9 |
| Poly-aspartic-acid | + | 5.7 |
Note：Dispersed to be represented with " ++ " by force, good dispersion is represented with "+", and dispersiveness is typically not done
As can be seen from Table 6, the dispersiveness of calcium lignosulfonate is best, next to that poly-aspartic-acid, lignin sulfonic acid
Sodium is general as the effect of dispersiveness.Next, we fix filler for kaolin and diatomaceous mixture, and both matter
Amount is than being 1:2, fixed wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, and both mass ratioes are 1:
1, fixed dispersant is calcium lignosulfonate, and the variety classes to stabilizer is tested, and the results are shown in Table 7,
Index Influence of the different stabilizers of table 7 to complex microorganism Wettable pesticide powder of the invention
As shown in Table 7, vitamin E is best as the effect of stabilizer, and at most, potassium dihydrogen phosphate is used as stabilization for number of viable
The effect of agent is not so good as vitamin E.From result above, filler is kaolin and diatomaceous mixture, and both quality
Than being 1:2, wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, and both mass ratioes are 1:1, dispersion
Agent is calcium lignosulfonate, and when stabilizer is vitamin E, obtained complex microorganism Wettable pesticide powder of the invention has
Optimal effect.
The effect experiment of curing the disease of the complex microorganism Wettable pesticide powder of the invention of embodiment 7
The complex microorganism Wettable pesticide powder of comparative example, itself and embodiment 4 are prepared according to the formula described in embodiment 4
Differ only in, the microbiology class in the complex microorganism Wettable pesticide powder of comparative example only contain golden yellow vertical strepto-
Bacterium fermentate, and do not contain rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder and bacillus
Mycelia, the composition for lacking uses filler polishing.
Next, answering using the complex microorganism Wettable pesticide powder described in embodiment of the present invention 1-4 and comparative example
Close Microbial wettable powder agricultural chemicals, commercially available carbendazim is used for the preventing and treating of banana blight together, such as many according to conventional suspension liquid
The application method and consumption of bacterium spirit, as a result see the table below：
The complex microorganism wettable powder agriculture of the complex microorganism Wettable pesticide powder of the invention of table 8 and comparative example
The prevention effect contrast experiment of medicine, carbendazim to banana blight
| Agricultural chemicals | Scab inhibiting rate | Integrated control effect |
| Embodiment 1 | 80% | 81% |
| Embodiment 2 | 82% | 82% |
| Embodiment 3 | 85% | 84% |
| Embodiment 4 | 88% | 87% |
| Comparative example | 72% | 74% |
| Carbendazim | 70% | 73% |
As can be seen from the above table complex microorganism Wettable pesticide powder of the invention and carbendazim, only containing golden yellow vertical
Streptomycete fermentation thing and do not contain rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder and gemma
The Microbial wettable powder agricultural chemicals of bacillus mycelia is compared, and complex microorganism Wettable pesticide powder of the invention can be more preferable
For preventing and treating banana blight, and the complex microorganism Wettable pesticide powder prevention effect of embodiment 4 is best, and it is comprehensively anti-
Control effect and be up to 80%, the scab inhibiting rate and integrated control effect of embodiment 3 are only second to embodiment 4.
Next, according to described in embodiment 4 formula prepare comparative example complex microorganism Wettable pesticide powder, its with
Embodiment 4 is differed only in, and the microbiology class in the complex microorganism Wettable pesticide powder of comparative example only contains Wuyi
Bacterium conidia powder, and do not contain golden yellow vertical streptomycete fermentation thing, rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder and
Bacillus mycelia, the composition for lacking uses filler polishing.
Then, the compound of the complex microorganism Wettable pesticide powder described in embodiment of the present invention 1-4 and comparative example is used
Microbial wettable powder agricultural chemicals, commercially available carbendazim are used for the preventing and treating of winter jujube rust together, according to conventional suspension liquid such as carbendazim
Application method and consumption, as a result see the table below：
The complex microorganism wettable powder agriculture of the complex microorganism Wettable pesticide powder of the invention of table 9 and comparative example
The prevention effect contrast experiment of medicine, carbendazim to winter jujube rust
| Agricultural chemicals | Scab inhibiting rate | Integrated control effect |
| Embodiment 1 | 82% | 82% |
| Embodiment 2 | 83% | 84% |
| Embodiment 3 | 85% | 86% |
| Embodiment 4 | 88% | 89% |
| Comparative example | 72% | 74% |
| Carbendazim | 68% | 72% |
As can be seen from the above table complex microorganism Wettable pesticide powder of the invention and carbendazim, only contain Wuyi bacterium spore
Sub- powder and do not contain golden yellow vertical streptomycete fermentation thing, rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder and gemma
The Microbial wettable powder agricultural chemicals of bacillus mycelia is compared, and complex microorganism Wettable pesticide powder of the invention can be more preferable
For preventing and treating winter jujube rust, and the complex microorganism Wettable pesticide powder prevention effect of embodiment 4 is best, its integrated control
Effect is up to 80%, and the scab inhibiting rate and integrated control effect of embodiment 3 are only second to embodiment 4.Next, according to embodiment
Formula described in 4 prepares the complex microorganism Wettable pesticide powder of comparative example, and it is differed only in embodiment 4, contrast
Microbiology class in the complex microorganism Wettable pesticide powder of example only contains rose yellow streptomycete mycelia, and does not contain golden yellow
Vertical streptomycete fermentation thing, Wuyi bacterium conidia powder, actinomyces styreptomyces globispotus strain original powder and bacillus mycelia, the composition for lacking are adopted
Use filler polishing.
Then, the compound of the complex microorganism Wettable pesticide powder described in embodiment of the present invention 1-4 and comparative example is used
Microbial wettable powder agricultural chemicals, commercially available carbendazim are used for the preventing and treating of cotton verticillium wilt together, according to for example many bacterium of conventional suspension liquid
The application method and consumption of spirit, as a result see the table below：
The complex microorganism wettable powder agriculture of the complex microorganism Wettable pesticide powder of the invention of table 10 and comparative example
The prevention effect contrast experiment of medicine, carbendazim to cotton verticillium wilt
| Agricultural chemicals | Scab inhibiting rate | Integrated control effect |
| Embodiment 1 | 83% | 82% |
| Embodiment 2 | 84% | 82% |
| Embodiment 3 | 86% | 85% |
| Embodiment 4 | 89% | 88% |
| Comparative example | 72% | 74% |
| Carbendazim | 68% | 72% |
As can be seen from the above table complex microorganism Wettable pesticide powder of the invention and carbendazim, only contain rose yellow chain
Fungal hyphae and do not contain golden yellow vertical streptomycete fermentation thing, Wuyi bacterium conidia powder, actinomyces styreptomyces globispotus strain original powder and gemma
The Microbial wettable powder agricultural chemicals of bacillus mycelia is compared, and complex microorganism Wettable pesticide powder of the invention can be more preferable
For preventing and treating cotton verticillium wilt, and the complex microorganism Wettable pesticide powder prevention effect of embodiment 4 is best, and it is comprehensively anti-
Control effect and be up to 86%, the scab inhibiting rate and integrated control effect of embodiment 3 are only second to embodiment 4.
In addition, present inventor also found complex microorganism Wettable pesticide powder of the invention except with very
Outside good preventing disease theraping effect, also have functions that to promote crop yield.
Prevent peanut dead leaf disease using complex microorganism Wettable pesticide powder of the invention, as a result it has also been found that of the invention
Complex microorganism Wettable pesticide powder can also be greatly facilitated peanut yield increasing, its result in addition to it can prevent and treat peanut dead leaf disease
See the table below：
Effect of increasing production experiment of the complex microorganism Wettable pesticide powder of the invention of table 11 with carbendazim to peanut
Complex microorganism Wettable pesticide powder of the invention is obvious to peanut yield increasing as can be seen from the above table, wherein implementing
Complex microorganism Wettable pesticide powder described in example 4 is most obvious to peanut yield increasing effect, the complex microorganism described in embodiment 3
Wettable pesticide powder takes second place to peanut yield increasing effect.
It is last to should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent, without deviating from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of complex microorganism Wettable pesticide powder, it is characterised in that it is made up of the component of following mass percent：Gold
Yellow vertical streptomycete fermentation thing 10%~15%, rose yellow streptomycete mycelia 10%~15%, actinomyces styreptomyces globispotus strain original powder
10%~15%, Wuyi bacterium conidia powder 10%~15%, bacillus mycelia 10%~15%, Choline Chloride 0.5%~2%,
Light rare earth 0.5%~2%, vitamins 0.5%~2%, trace element 0.5%~2%, wetting agent 2%~5%, dispersant
2%~5%, stabilizer 2%~5%, filler is supplied to 100%.
2. a kind of complex microorganism Wettable pesticide powder according to claim 1, it is characterised in that described golden yellow vertical
Streptomycete fermentation thing is prepared by following steps：1) golden yellow vertical streptomycete is activated：Golden yellow vertical streptomycete is connect with method of scoring
Plant on Gause I culture medium flat plate, 28 DEG C incubated 4 days, the mass percent composition of culture medium：Soluble starch
2.5%, KNO30.2%th, K2HPO40.05%th, MgSO40.05%th, NaCl 0.05%, FeSO40.05%th, agar 2.5%,
Moisturizing is to 100%；2) the golden yellow vertical streptomycete for activating is inoculated in first order seed nutrient solution, 180rmin-1, 28 DEG C of trainings
Support 72h, the mass percent composition of first order seed nutrient solution：Soluble starch 2.5%, KNO30.2%, K2HPO40.05%th,
MgSO40.05%th, NaCl 0.05%, FeSO40.05%, moisturizing to 100%；3) the first order seed nutrient solution that will be prepared connects
Plant in secondary seed nutrient solution, 180rmin-1, 28 DEG C are cultivated 72h, and centrifugation obtains golden yellow vertical streptomycete fermentation
Thing, low temperature drying, moisture is less than 5%, and the mass percent of secondary seed nutrient solution is constituted：It is glucose 0.05%, solvable
Property starch 0.05%, K2HPO40.05%th, NaCl 0.05%, MgSO40.05%th, peptone 0.05%, the and of yeast extract 0.05%
Analysis for soybean powder 0.05%, moisturizing to 100%；
The rose yellow streptomycete mycelia is prepared by following steps：1) by rose yellow streptomycete in 28 DEG C of trainings in PDA culture medium
Support 5 days；2) and then with 2% ratio it is inoculated into seed culture medium, condition of culture is：28℃、180r·min-1, cultivate 3 days,
Wherein seed culture medium is：3.5% groundnut meal, 5% starch, 1% glucose, 0.8% sodium chloride, 0.3% alcohol mother's cream,
0.8% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.4% calcium carbonate, 0.2% ferrous sulfate, 0.2% zinc sulfate, 3.5% sucrose,
0.4% fructose；3) the rose yellow streptomycete culture for preparing step (2) is by being filtrated to get the rose yellow streptomycete
Mycelia；
The actinomyces styreptomyces globispotus strain original powder is prepared by following steps：1) the actinomyces styreptomyces globispotus strain that will have been activated with
2% ratio is inoculated into and is inoculated in Gause I fluid nutrient medium, 28 DEG C, 150-160rmin-1Obtained after shaken cultivation 120h
Actinomyces seed culture fluid；2) the actinomyces styreptomyces globispotus strain for preparing step (2) is by filtering, being dried to obtain described putting
Line bacterium styreptomyces globispotus strain original powder；
The bacillus mycelia is prepared by following steps：The bacillus that will have been activated is inoculated in training in 2% ratio
Support in base, cultivated 5 days at 28 DEG C, take out culture and filtered, the solid for obtaining as bacillus mycelia；It is wherein described
Bacillus is bacillus licheniformis, bacillus subtilis or wax bacillus.
3. a kind of complex microorganism Wettable pesticide powder according to claim 1, it is characterised in that it is by following quality
The component of percentage is made：Golden yellow vertical streptomycete fermentation thing 10%~12%, rose yellow streptomycete mycelia 10%~12%, put
Line bacterium styreptomyces globispotus strain original powder 10%~12%, Wuyi bacterium conidia powder 10%~12%, bacillus mycelia 10%~12%, chlorine
Change choline 0.5%~1%, light rare earth 0.5%~1%, vitamins 0.5%~1%, trace element 0.5%~1%, wetting
Agent 2%~4%, dispersant 2%~4%, stabilizer 2%~4%, filler are supplied to 100%.
4. a kind of complex microorganism Wettable pesticide powder according to claim 1, it is characterised in that it is by following quality
The component of percentage is made：Golden yellow vertical streptomycete fermentation thing 10%, rose yellow streptomycete mycelia 10%, actinomyces ball spore strepto-
Bacterium original powder 10%, Wuyi bacterium conidia powder 10%, bacillus mycelia 10%, Choline Chloride 0.5%, light rare earth 0.5%, vitamin
Class 1%, trace element 1%, wetting agent 3%, dispersant 3%, stabilizer 3%, filler are supplied to 100%.
5. a kind of complex microorganism Wettable pesticide powder according to claim 1, it is characterised in that the vitamins
It is at least one in vitamin B or C；It is described trace element be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo, Cu, Zn and
The mass ratio of Fe is 4：5：2：5：2：7, B, Mn, Mo, Cu, Zn and Fe come from following raw material：Borax, manganese sulfate, sodium molybdate,
Copper sulphate, zinc sulfate, ferrous sulfate；The wetting agent is in lauryl sodium sulfate, Tween-80, sodium methylene bis-naphthalene sulfonate
At least one；The dispersant is at least one in sodium lignin sulfonate, sodium lignin sulfonate calcium, poly-aspartic-acid；
The stabilizer is at least one in vitamin E, potassium dihydrogen phosphate；Described filler is in diatomite, bentonite, kaolin
It is at least one.
6. a kind of complex microorganism Wettable pesticide powder according to claim 5, it is characterised in that the vitamins
It is vitamin B and the mixture of C, both mass ratioes are 1：1, the wetting agent is Tween-80 and sodium methylene bis-naphthalene sulfonate
In mixture, wherein both mass ratio be 1:1；The dispersant is calcium lignosulfonate；The stabilizer is vitamin
E；Described filler is kaolin and diatomaceous mixture, and wherein both mass ratioes are 1:2.
7. a kind of complex microorganism Wettable pesticide powder according to claim 1, it is characterised in that it is by following quality
The component of percentage is made：Golden yellow vertical streptomycete fermentation thing 15%, rose yellow streptomycete mycelia 12%, actinomyces ball spore strepto-
Bacterium original powder 15%, Wuyi bacterium conidia powder 12%, bacillus mycelia 15%, Choline Chloride 1%, light rare earth 2%, vitamins
2%th, trace element 1%, wetting agent 4%, dispersant 4%, stabilizer 4%, filler are supplied to 100%；Wherein vitamins is
The mixture of vitamin B and C, both mass ratioes are 1：1；Trace element be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo,
The mass ratio of Cu, Zn and Fe is 4：5：2：5：2：7, B, Mn, Mo, Cu, Zn and Fe come from following raw material：Borax, manganese sulfate,
Sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is sodium methylene bis-naphthalene sulfonate；Dispersant is poly-aspartic-acid；
Stabilizer is the mixture in vitamin E and potassium dihydrogen phosphate, and wherein both mass ratioes are 1:1；Filler is bentonite and height
The mixture of ridge soil, wherein both mass ratioes are 1:1.
8. a kind of complex microorganism Wettable pesticide powder according to claim 1, it is characterised in that it is by following quality
The component of percentage is made：Golden yellow vertical streptomycete fermentation thing 10%, rose yellow streptomycete mycelia 10%, actinomyces ball spore strepto-
Bacterium original powder 10%, Wuyi bacterium conidia powder 10%, bacillus mycelia 10%, Choline Chloride 0.5%, light rare earth 0.5%, vitamin
Class 1%, trace element 1%, wetting agent 3%, dispersant 3%, stabilizer 3%, filler are supplied to 100%；Wherein described dimension life
Plain class is the mixture of vitamin B and C, and both mass ratioes are 1：1：1；It is described trace element be B, Mn, Mo, Cu, Zn and Fe,
Wherein the mass ratio of B, Mn, Mo, Cu, Zn and Fe is 4：5：2：5：2：7, B, Mn, Mo, Cu, Zn and Fe come from following raw material：
Borax, manganese sulfate, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is in Tween-80 and sodium methylene bis-naphthalene sulfonate
Mixture, wherein both mass ratio be 1:1；Dispersant is calcium lignosulfonate；Stabilizer is vitamin E；Described filler
It is kaolin and diatomaceous mixture, wherein both mass ratioes are 1:2.
9. a kind of preparation method of complex microorganism Wettable pesticide powder as described in any one of claim 1~8, its feature
It is that the described method comprises the following steps：According to the ratio by wetting agent, dispersant, stabilizer, filler, Choline Chloride, light
Rare earth is crushed in putting into airslide disintegrating mill, crosses 350 mesh sieves, after cooling, adds golden yellow vertical streptomycete fermentation thing, rose yellow chain
Fungal hyphae, actinomyces styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder, bacillus mycelia, vitamins, trace element, after
Continued powder is broken, obtains the complex microorganism Wettable pesticide powder.
10. the complex microorganism Wettable pesticide powder described in any one of claim 1~8 is in preventing and treating banana blight, winter jujube
Purposes in rust, cotton verticillium wilt and peanut yield increasing.
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| CN112889842A (en) * | 2021-01-22 | 2021-06-04 | 中国烟草中南农业试验站 | Wettable bacterium powder and preparation method and application thereof |
| EP3872167A4 (en) * | 2018-10-26 | 2023-05-03 | Agrivalle Brasil Industria e Comércio de Produtos Agrícolas Ltda | Multiple-function biological composition |
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| CN102206593A (en) * | 2011-03-15 | 2011-10-05 | 海南大学 | Streptomyces aureoverticillatus strain and its application |
| CN102381912A (en) * | 2011-11-09 | 2012-03-21 | 潍坊万胜生物农药有限公司 | Microbial pesticide preparation for plant protection and growth regulation |
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