CN106305792B - A kind of wettable powder that Gliocladium roseum is compounded with bacillus subtilis - Google Patents
A kind of wettable powder that Gliocladium roseum is compounded with bacillus subtilis Download PDFInfo
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- CN106305792B CN106305792B CN201610331376.XA CN201610331376A CN106305792B CN 106305792 B CN106305792 B CN 106305792B CN 201610331376 A CN201610331376 A CN 201610331376A CN 106305792 B CN106305792 B CN 106305792B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Abstract
The present invention provides the wettable powders that a kind of mould fungal spore powder of viscous broom with fungal diseases such as prevention and treatment gray mold, banded sclerotial blight, root rot, fungal leaf spot, sclerotinioses and the bacillus subtilis bacterial spore powder with bacterial diseases such as prevention and treatment bacterial wilt, soft rot, canker, bacterial speck, bacterial angular leaf spots compound.The wettable powder is made of following raw material in parts by weight: the mould fungal spore powder of 50-100 parts of viscous brooms, 50-100 parts of bacillus subtilis bacterial spore powder, 800-850 parts of diatomite, 10-20 parts of neopelexes, 10-50 parts of sodium lignin sulfonates, 10-50 parts of sodium carboxymethylcelluloses, 5-10 parts of ascorbic acid.Wettable powder compounding microorganism provided by the present invention has broad-spectrum without mutual antagonism, to crop, application personnel and Environmental security, improves crop quality, improves yield, the stable feature of performance.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of viscous broom with prevention and treatment fungal disease is mould
(Gliocladium roseum) fungal spore powder and with prevention and treatment bacterial disease bacillus subtilis (Bacillus
Subtilis) the method for the wettable powder of bacterial spore powder compounding.
Background technique
Since chemical fertilizer, chemical pesticide, and the tillage method of high-intensitive multiple cropping, continuous cropping production is used for a long time, cause
Occurring such as pest and disease damage resistance enhancing in China's agricultural production process, soil continuous production ability and utilization rate of fertilizer reduce,
Farmland quality by destroy, organic matter deficiency, soil hardening, fertility decline, soil-borne disease and continuous cropping obstacle take place frequently, agricultural production quality
A series of the problem of amount safety problem such as gets worse at restriction China's agricultural developments.And by the microorganism fungus kind with biological and ecological methods to prevent plant disease, pests, and erosion function
The microbial manure and microbial pesticide prepared by biofermentation technique and formulation technology, due to diseases prevention, growth-promoting,
Rehabilitating soil Tiny ecosystem, dissolving phosphor and dissolving potassium, improves the functions such as utilization rate of fertilizer at improvement soil texture, can be above-mentioned with effective solution
The problem of restricting China's agricultural development.
With the development of biofermentation technique and formulation technology, China's microbial manure industry and microbial pesticide are produced
Industry is also grown rapidly, especially in nearest twenty or thirty year, the product category of microbial manure and microbial pesticide,
Year output and manufacturing enterprise's quantity have been greatly improved, and when the two industries are fast-developing, there is also controlling disease phases
To single, prevention spectrum relative narrower, protection effect is to be improved, strain is easily degenerated, environmental factor dependence is strong the problems such as, at present in agriculture
Single culture, list in the microbial manure and microbial pesticide of industry portion microbial manure center and the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC's registration
The product accounting of one function is more than 80%, and wettable powder of the invention is by two kinds of different microbial inoculums for preventing and treating mechanism and wetting
Agent, dispersing agent, uv-protector, carrier are process, and are had preferable suspended dispersed performance and water solubility, can both be prevented and treated leaf
Portion's disease can also prevent and treat the disease in soil, and method of administration is versatile and flexible, root trickle irrigation can be used, punching is applied, pouring root and ground
A variety of fungies and bacteriosis are all had good control efficiency by the method for administration such as the spraying, spray in portion.
Summary of the invention
The present invention provides one kind to have prevention and treatment gray mold, banded sclerotial blight, root rot, fungal leaf spot, sclerotiniose etc. true
The mould fungal spore powder of the viscous broom of fungal disease and there is prevention and treatment bacterial wilt, soft rot, canker, bacterial speck, bacillary
The wettable powder and preparation method thereof of the bacillus subtilis bacterial spore powder compounding of the bacterial diseases such as angular leaf spot.
Above-mentioned wettable powder is by gluing the mould fungal spore powder of broom, bacillus subtilis bacterial spore powder, diatomite, 12
Sodium alkyl benzene sulfonate, sodium lignin sulfonate, sodium carboxymethylcellulose, ascorbic acid are mixed and processed, according to parts by weight, respectively
Component is respectively as follows: the mould fungal spore powder of 50-100 parts of viscous brooms, 50-100 parts of bacillus subtilis bacterial spore powder, 800-850 parts
Diatomite, 10-20 part neopelex, 10-50 parts of sodium lignin sulfonates, 10-50 parts of sodium carboxymethylcelluloses, 5-10
Part ascorbic acid.
The wettable powder the preparation method comprises the following steps:
Step 1: by concentration be cfu/ grams of 5-10 hundred million the mould fungal spore powder of viscous broom (purity be greater than 90%, water content is less than
10%, chlamydospore ratio be greater than 30%), concentration be cfu/ grams of 200-300 hundred million bacillus subtilis bacterial spore powder (purity
Greater than 90%, water content is sequentially added in mixing machine less than 10%) with diatomite, after mixing 0.5~1h, sequentially adds 12
Sodium alkyl benzene sulfonate, sodium lignin sulfonate, sodium carboxymethylcellulose, ascorbic acid are uniformly mixed after mixing 0.5~1h
Object；
Step 2: the mixture of previous step is transferred to low-temperature airflow pulverizer, temperature control is crushed at 20 DEG C hereinafter, crushing
300 meshes are crossed afterwards, obtain dispersibility and the good wettable powder of suspension.
Above-mentioned Gliocladium roseum bacterial strain (Gliocladium roseum), number QGSS-C16 are isolated from Shandong Shouguang
The village Dong Zhenguan of city tomato plastic shed soil.The bacterial strain has been preserved in Chinese microorganism strain preservation management committee on 04 25th, 2016
Member's meeting common micro-organisms center (Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), preservation registration number are CGMCC No.12243.
Bacterial strain is suitble to the culture medium of growth to be PDA culture medium, and being suitble to the pH of growth is 6.0~6.8, and being suitble to the temperature of growth is 25~28
℃。
Above-mentioned bacillus subtilis strain (Bacillus subtilis), number QGSS-05 are isolated from Shandong Shouguang
Ternary Zhu Cun.The bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on October 30th, 2015
Center (Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), preservation registration number are CGMCC No.11563.Bacterial strain is suitble to growth
Culture medium is NA culture medium, and being suitble to the pH of growth is 7.0~7.2, and being suitble to the temperature of growth is 28~30 DEG C.
Wettable powder energy quick, uniform suspended dispersed provided by the invention with disease prevention growth-promoting is in water, this hair
Bright wettable powder has the special feature that and advantage are as follows:
(1) by mutually the biocontrol fungi of antagonism is not compounded with bacterium, good synergistic effect is played, expands and disease is prevented
Range is controlled, solves the problems, such as that single bio-control factors diseases prevention is composed narrow, ensure that the control efficiency to different type disease；
(2) method of administration is versatile and flexible, root trickle irrigation can be used, the applications side such as punching is applied, pouring root and overground part are spraying, spray
Method；
(3) Gliocladium roseum and bacillus subtilis added is isolated from soil to have higher biological and ecological methods to prevent plant disease, pests, and erosion living
Property biocontrol bacterial strain, have that resistance is strong, nutritional requirement is simple, is easy to colonize, in plant rhizosphere and blade face to various crop
Bacillary and fungal disease has many advantages, such as preferable control efficiency；
(4) ratio for gluing chlamydospore in the mould conidia powder of broom is greater than 30%, substantially increases the time-to-live of fungal spore,
Extend shelf life；
(5) bacterial strain uses therefor derives from soil, high to crop, application personnel, environmental safety.
Specific embodiment
Embodiment 1
Step 1: by concentration be 1,000,000,000 cfu/ grams 100 parts of the mould fungal spore powder of viscous broom, concentration be 30,000,000,000 cfu/ grams
50 parts of bacillus subtilis bacterial spore powder sequentially add in mixing machine with 815 parts of diatomite, after mixing 0.5~1h, then successively
10 parts of neopelex, 10 parts of sodium lignin sulfonate, 10 parts of sodium carboxymethylcellulose, 5 parts of ascorbic acid, mixing is added
Uniform mixture is obtained after 0.5~1h；
Step 2: the mixture of previous step is transferred to low-temperature airflow pulverizer, temperature control is crushed at 20 DEG C hereinafter, crushing
300 meshes are crossed afterwards, obtain dispersibility and the good wettable powder of suspension.
Suspensibility is measured according to 4.1 in agricultural-chemical suspension rate measuring method GB/T 14825-2006, and wetting time is pressed
It is measured according to pesticide wettable wettability determination method GB/T 5451-2001, fineness is according to pesticide powder, wettable
2.2 wet screenings in pulvis fineness measuring method GB/T 16150-1995 are measured, and moisture is according to pesticide moisture measuring method
2.2 azeotropic distillation methods are measured in GB/T 1600-2001, pH value according to pesticide pH value measuring method GB/T 1601-
1993 are measured.The suspensibility for being detected the wettable powder is 78%, and wetting time 83s, fineness 99% passes through 48 μm
Sieve, moisture 6.3%, pH6.9.
Embodiment 2
Step 1: by concentration be 1,000,000,000 cfu/ grams 50 parts of the mould fungal spore powder of viscous broom, concentration be 30,000,000,000 cfu/ grams withered
50 parts of careless bacillus bacterial spore powder sequentially add in mixing machine with 835 parts of diatomite, after mixing 0.5~1h, then successively add
Enter 15 parts of neopelex, 15 parts of sodium lignin sulfonate, 25 parts of sodium carboxymethylcellulose, 10 parts of ascorbic acid, mixes
Uniform mixture is obtained after 0.5~1h；
Step 2: the mixture of previous step is transferred to low-temperature airflow pulverizer, temperature control is crushed at 20 DEG C hereinafter, crushing
300 meshes are crossed afterwards, obtain dispersibility and the good wettable powder of suspension.
Suspensibility is measured according to 4.1 in agricultural-chemical suspension rate measuring method GB/T 14825-2006, and wetting time is pressed
It is measured according to pesticide wettable wettability determination method GB/T 5451-2001, fineness is according to pesticide powder, wettable
2.2 wet screenings in pulvis fineness measuring method GB/T 16150-1995 are measured, and moisture is according to pesticide moisture measuring method
2.2 azeotropic distillation methods are measured in GB/T 1600-2001, pH value according to pesticide pH value measuring method GB/T 1601-
1993 are measured.The suspensibility for being detected the wettable powder is 75%, and wetting time 80s, fineness 99% passes through 48 μm
Sieve, moisture 6.8%, pH6.7.
Embodiment 3
Step 1: by concentration be 500,000,000 cfu/ grams 100 parts of the mould fungal spore powder of viscous broom, concentration be 20,000,000,000 cfu/ grams withered
100 parts of careless bacillus bacterial spore powder sequentially add in mixing machine with 815 parts of diatomite, after mixing 0.5~1h, then successively add
Enter 20 parts by weight of neopelex, 20 parts by weight of sodium lignin sulfonate, 50 parts by weight of sodium carboxymethylcellulose, Vitamin C
Sour 10 parts by weight, obtain uniform mixture after mixing 0.5~1h；
Step 2: the mixture of previous step is transferred to low-temperature airflow pulverizer, temperature control is crushed at 20 DEG C hereinafter, crushing
300 meshes are crossed afterwards, obtain dispersibility and the good wettable powder of suspension.
Suspensibility is measured according to 4.1 in agricultural-chemical suspension rate measuring method GB/T 14825-2006, and wetting time is pressed
It is measured according to pesticide wettable wettability determination method GB/T 5451-2001, fineness is according to pesticide powder, wettable
2.2 wet screenings in pulvis fineness measuring method GB/T 16150-1995 are measured, and moisture is according to pesticide moisture measuring method
2.2 azeotropic distillation methods are measured in GB/T 1600-2001, pH value according to pesticide pH value measuring method GB/T 1601-
1993 are measured.The suspensibility for being detected the wettable powder is 80%, and wetting time 75s, fineness 99% passes through 48 μm
Sieve, moisture 6.1%, pH6.5.
Embodiment 4
The field experiment of wettable powder prevention and treatment graw mold of tomato:
Changping County, Beijing Bai Shan town lion on April 2nd, 2015 seeks village's day-light greenhouse, and experimental field preceding stubble plants tomato, has
The generation of gray mold.The purpose of the experiment is to prevent and treat the generation of graw mold of tomato.The wettable powder that will be prepared in embodiment 1,2,3
500 times of liquid of agent carry out foliage-spray as experiment process, spray 50% iprodione wettable powder of chemical pesticide, 1000 times of liquid and make
Positive control sprays clear water and makees blank control, and each processing sets 3 repetitions, per duplicate plot area be 30 square metres, kind
Eggplant Post flowering starts to spray, and sprays every 7 days 1 time, continuous spraying 3 times, is spaced 7 days investigation incidences after spraying for last 1 time.
Field management is carried out by production Routine Management mode, mark is carried out with the method for being red rope to the plant that will be investigated before the onset
Graw mold of tomato disease index is investigated in note, 20 plants of every duplication, is recorded total number of sheets, the sick number of sheets at different levels of tomato, is calculated the state of an illness
Index and control efficiency.Each cell production is recorded after each fruit picking, latter stage is harvested in tomato, calculates each cell total output and convert into
For per mu yield.
Disease index grade scale: 0 grade, disease-free spot；
1 grade, lesion area accounts for 5% or less entire leaf area；
3 grades, lesion area accounts for the 6%-10% of entire leaf area；
5 grades, lesion area accounts for the 11%-25% of entire leaf area；
7 grades, lesion area accounts for the 26%-50% of entire leaf area；
9 grades, lesion area accounts for 50% or more of entire leaf area.
Disease index (%)=∑ (the disease numbers of sheets at different levels × opposite value of series)/(investigation total number of sheets × highest disease grade value) ×
100。
Control efficiency (%)=(blank district disease index-treatment region disease index)/blank district disease index
×100。
Control efficiency of 1 wettable powder of table to gray mold and the influence to tomato yield
Embodiment 5
The field experiment of wettable powder prevention and treatment pepper ralstonia solanacearum:
The March in 2015 of Langfang in Hebei Province on the 22nd cooks a meal Guangyang District village village day-light greenhouse, tests the continuous three batches of plantations phase in plot
With kind capsicum, there is bacterial wilt, there are Soil-sickness Problems.
The purpose of the experiment is to prevent and treat the generation of pepper ralstonia solanacearum.The wettable powder 500 that will be prepared in embodiment 1,2,3
Times liquid carries out pouring root as experiment process, and 3% 600 times of Zhongshengmycin wet powder liquid irrigating root makees positive control, fills clear water and makees sky
White control, each processing set 3 repetitions, are 30 square metres per duplicate plot area, medication before the onset of bacterial wilt, every 7
Its pouring root 1 time, it is continuous to fill 4 times, 7 days investigation incidences are spaced after last 1 pouring root.Field is carried out by production Routine Management mode
Between manage, the plant that will be investigated is marked with the method for being red rope before the onset, 40 plants of every duplication, investigate capsicum
Dead strain number calculates the death rate and control efficiency, each cell production is recorded after each fruit picking, harvests latter stage in capsicum, calculates each
Cell total output is simultaneously converted into per mu yield.
Control efficiency of 2 wettable powder of table to pepper ralstonia solanacearum and the influence to yield of hot pepper
From greenhouse test embodiment 4,5 as can be seen that wettable powder prepared by the present invention is to gray mold fungal disease
Good control efficiency is all had with bacterial wilt bacterial disease, and plant growing way is more obvious than the control of conventional chemical medicament prosperous
It contains, the minimum volume increase 14.36% of tomato, the minimum volume increase 11.95% of capsicum can be made.
Claims (2)
1. the wettable powder that a kind of Gliocladium roseum is compounded with bacillus subtilis, which is characterized in that the wettable powder
It is made of following raw material in parts by weight: the mould fungal spore powder of 50-100 parts of viscous brooms, 50-100 parts of bacillus subtilis bacterium buds
Cryptogam, 800-850 part diatomite, 10-20 parts of neopelexes, 10-50 parts of sodium lignin sulfonates, 10-50 parts of carboxylic first
Base sodium cellulosate, 5-10 parts of ascorbic acid compoundings are process；
The mould fungal spore powder purity of viscous broom is greater than 90%, and for water content less than 10%, chlamydospore ratio is greater than 30%；
The mould fungal spore powder of viscous broom is the viscous mould QGSS-C16 conidia powder of broom, and deposit number is CGMCC No.12243；
The bacillus subtilis spore powder is bacillus subtilis QGSS-05 gemma powder, and deposit number is CGMCC No.11563.
2. the wettable powder that Gliocladium roseum according to claim 1 is compounded with bacillus subtilis, which is characterized in that
The bacillus subtilis spore powder purity is greater than 90%, and water content is less than 10%.
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| BR102018010543A2 (en) * | 2018-05-23 | 2019-12-10 | Agrivalle Brasil Ind E Comercio De Produtos Agricolas Ltda | composition for biological control of phytopathogenic fungi |
| CN110129242B (en) * | 2019-06-13 | 2021-07-13 | 北京市农林科学院 | Continuous cropping resistant composite microbial preparation and preparation method thereof |
| CN110558336A (en) * | 2019-07-19 | 2019-12-13 | 湖南科技学院 | Biocontrol agent for preventing and treating lettuce sclerotinia rot and preparation and using method thereof |
| CN111471598B (en) * | 2020-04-25 | 2021-02-19 | 甘肃省科学院生物研究所 | Gliocladium roseum and pimavalia rimonaris composite microbial agent and application thereof in prevention and control of diseases and pests |
| CN114262248A (en) * | 2022-01-13 | 2022-04-01 | 宁波市农业科学研究院 | Biological bacterial fertilizer for preventing and treating soft rot of pakchoi and preparation method thereof |
| CN115029286B (en) * | 2022-07-21 | 2023-06-20 | 东北农业大学 | Biocontrol bacterium compound microbial inoculum and application thereof |
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