CN104026154A - Gliocladium rosea conidia powder dry suspension bactericide and application thereof - Google Patents
Gliocladium rosea conidia powder dry suspension bactericide and application thereof Download PDFInfo
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- CN104026154A CN104026154A CN201410300518.7A CN201410300518A CN104026154A CN 104026154 A CN104026154 A CN 104026154A CN 201410300518 A CN201410300518 A CN 201410300518A CN 104026154 A CN104026154 A CN 104026154A
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- broom bacterium
- bactericide
- parasitism broom
- parasitism
- dried bean
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a gliocladium rosea conidia powder dry suspension bactericide and application thereof. According to the formula, the bactericide is prepared from the following components in mass percent: 5%-30% of gliocladium rosea conidia powder, 8%-20% of wetting dispersant, 0.1%-1% of ultraviolet protective agent, 2%-20% of disintegrant, 1.0%-10% of binder and 19%-83.9% of carrier. The gliocladium rosea conidia powder dry suspension bactericide is used for preventing and controlling diseases of pathogenic apple ring spot and sclerotinia rot of colza in agriculture and forestry. According to the gliocladium rosea conidia powder dry suspension bactericide, the germination rate and the suspension rate of conidia are improved, the wetting speed and the disintegration speed are high, and the dispersibility and the thermal stability are excellent. The gliocladium rosea conidia powder dry suspension bactericide has the advantages of high conidia survival rate (80.16%), long storage life, fast disintegration, high suspension rate, small grain size, strong adhesive force, and washing resistance, and the bactericide is an excellent green pesticide.
Description
Technical field
The present invention relates to a kind of microbial pesticide, specifically its application of a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide.
Background technology
Parasitism broom bacterium (Clonostachys rosea), is subordinate to hyphomycete, is the Hyperparasite that a class is extensively present in the plant pathogenic fungi in soil.T.Yh-Mattila etc. research finds that it is containing widely distributed in the soil of fertilizer.Mycelia and sclerotium that can parasitic various plants disease fungus also have the effect of Promoting plant growth simultaneously.
Parasitism broom bacterium can belong to the various plants pathogens such as (Sclerotinia), pythium spp (Pythium debaryanum), Rhizoctonia solani Kuhn (Rhizoctonia solani) and Sclerotium rolfsii (Sclerotium rolfsii) by Sclerotinia sclerotiorum.Sticky broom bacterium has the multiple action mechanism such as the antibiotic of generation and toxin, hyperparasitism, generation cell wall degrading enzyme and synergy to phytopathogen, can effectively suppress fungal diseases of plants generation.The report of the sticky broom bacterium granule of external existing commercialization, and different strains is made to biologic product, for preventing and treating stalk break and other multiple greenhouse and the field crop soil-borne diseases such as pea, soybean, sunflower, rape, lettuce.Dry suspending agent is pesticides new formulation and the novel formulation process technology that starts in the world research and development the eighties in 20th century.This formulation has the graininess formulation that is dispersed into the solid-liquid dispersing system of higher suspension into the rapid disintegration of water, and or not containing organic solvent, suspension stability is not good, suspensibility is high, without dust, and packaging, transport, easy to use.Dry suspending agent is considered to both have advantages of granula, has again desirable formulations of pesticide of suspending agent high dispersive performance, has become the focus of novel form exploitation.Because Parasitism broom bacteria strain is poor to the biocompatibility of auxiliary agent, widely apply in so far there are no the market sale of its commodity preparation and field.
Summary of the invention:
Lack at present sticky broom microbial inoculum type, the invention provides its application of a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide.The present invention utilizes its conidial powder to make water slurry, water slurry through moment mist projection granulating be prepared into dry suspending agent.
The technology used in the present invention is as follows: a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide, it is as follows that formula is pressed mass percent: Parasitism broom bacterium conidial powder 5～30%, wetting dispersing agent 8～20%, uv-protector 0.1～1%, disintegrant 2～20%, binding agent 1.0～10% and carrier 19～83.9%.
The present invention also has following technical characterictic:
1. described in, carrier is a kind of or its composition in attapulgite, kaolin, diatomite, bentonite, white carbon.
2. selected wetting dispersing agent is a kind of or its composition in Morwet EFW, k12, MF-5, TERWET1010, WLNO, calcium lignosulfonate, TERSPERSE2700, TERSPERSE2020, Morwet D425, NNO, T36.
3. selected disintegrant is a kind of or its composition in crospolyvinylpyrrolidone, Ac-Di-Sol, PVPP, urea, ammonium sulfate, starch.
4. selected binding agent is a kind of or its composition in CMC, sodium lignin sulfonate, PEG, oxalic acid, dextrin.
5. selected uv-protector is a kind of or its composition of dextrin, xanthans, fluorescein sodium, vitamin C, nano zine oxide.
6. Parasitism broom bacterium conidial powder spore content>=1 × 10 described in
10individual/g.
7. to press mass percent proportioning as follows for optimization formula: mixture 12%, crospolyvinylpyrrolidone 3%, nano zine oxide 0.2%, PEG4% and the white carbon 75.8% of Parasitism broom bacterium conidial powder 5%, Morwet EFW and calcium lignosulfonate, wherein, Morwet EFW is 1: 5 with the mixture quality ratio of calcium lignosulfonate.
8. to press mass percent proportioning as follows for optimization formula: mixture 15%, the PVPP 4% of Parasitism broom bacterium conidial powder 20%, TERWET1010 and TERSPERSE2700, vitamin C 0.3%, sodium lignin sulfonate 5% and diatomite 55.7%, wherein the mixture quality of TERWET1010 and TERSPERSE2700 ratio is 1: 4.
9, described a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide is as the application of the disease of the control pathogenic ring rot of apple of agricultural and sclerotinia rot of colza.
The present invention is Parasitism broom bacterium dry suspending agent, has improved conidial germination rate and suspensibility, and wetting fast with disintegration rate, dispersiveness and heat storage stability are good, for control of plant disease provides new material.Have conidium survival rate high (80.16%), long shelf-life, disintegration fast, suspensibility is high, particle diameter is thin, and the advantages such as strong adhesion, resistance to erosion are a kind of good environment friendly agriculturals.
Brief description of the drawings
Fig. 1 Parasitism broom bacterium conidium figure;
Fig. 2: the particle diameter distribution map of Parasitism broom bacterium dry suspending agent.
Embodiment
In this test, Parasitism broom bacterium (Clonostachys rosea) is Parasitism broom bacterium by morphology and ITS-rDNA Sequence Identification, accession number: KJ941018.Derive from the State Administration of Forestry's 948 projects, problem title: gray mold antagonistic microbe production technology is introduced.
Embodiment 1
Parasitism broom bacterium conidial powder 5%, Morwet EFW and lignin sulfonic acid calcium compound 12%, crospolyvinylpyrrolidone 3%, PEG4%, nano zine oxide 0.2%, white carbon 75.8%, wherein, Morwet EFW and calcium lignosulfonate mixture quality ratio are 1: 5;
Embodiment 2
Parasitism broom bacterium conidial powder 20%, TERWET1010 and TERSPERSE2700 mixture 15%, PVPP 4%, sodium lignin sulfonate 5%, vitamin C 0.3%, diatomite 55.7%, wherein, TERWET1010 and TERSPERSE2700 mixture quality ratio are 1: 4;
Embodiment 3
Parasitism broom bacterium conidial powder 25%, TERWET1010, TERSPERSE2020 and T36 mixture 18%, ammonium sulfate 5%, CMC6%, nano zine oxide 0.4%, bentonite 45.6%, wherein, TERWET1010, TERSPERSE2020 and T36 mixture quality ratio are 1: 3: 2;
Embodiment 4
Parasitism broom bacterium conidial powder 7%, Morwet EFW and lignin sulfonic acid calcium compound 10%, urea 2%, oxalic acid 1%, xanthans 0.3%, kaolin and white carbon mixture 79.7%, wherein, Morwet EFW and calcium lignosulfonate mixture quality ratio are 1: 4, and kaolin and white carbon mixture quality ratio are 1: 4;
Embodiment 5
Parasitism broom bacterium conidial powder 28%, TERWET1010, TERSPERSE2020 and T36 mixture 16%, crospolyvinylpyrrolidone and PVPP mixture 20%, sodium lignin sulfonate and PEG mixture 10%, fluorescein sodium 0.6%, diatomite and white carbon mixture 25.4%, wherein, TERWET1010, TERSPERSE2020, T36 mixture quality ratio is 1: 1: 2, crospolyvinylpyrrolidone and PVPP mixture quality ratio are 1: 3, sodium lignin sulfonate and PEG mixture quality ratio are 1: 4, diatomite and white carbon mixture quality ratio are 1: 1,
Embodiment 6
Parasitism broom bacterium conidial powder 30%, TERWET1010, TERSPERSE2020 and T36 mixture 20%, crospolyvinylpyrrolidone and crosslinked sodium carboxymethylcellulose pyce mixture 18%, dextrin and oxalic acid mixture 9%, nano zine oxide and xanthan gum mixtures 1%, bentonite and white carbon mixture 22%, wherein, TERWET1010, TERSPERSE2020 and T36 mixture quality ratio are 2: 3: 5, crospolyvinylpyrrolidone and crosslinked sodium carboxymethylcellulose pyce mixture quality ratio are 1: 2, dextrin and oxalic acid mixture quality ratio are 2: 1, nano zine oxide and xanthan gum mixtures mass ratio are 2: 3, bentonite and white carbon mixture quality ratio are 1: 3,
Embodiment 7
Press said components and proportioning after Universalpulverizer mechanical mixture even (except conidial powder), add appropriate water, carry out micronization through homogenizer or sand mill, add conidial powder, shear conidial powder is dispersed in the aqueous solution of said components through high-speed shearing machine, then carry out mist projection granulating.
Step 1: according to weight percent meter, each auxiliary agent and carrier, through Universalpulverizer premix, add after appropriate water, then through Canadian ATS high pressure homogenizer (model AH100B) or sand mill micronization, operating pressure 500～1200bar, particle diameter is controlled at 25 μ m;
Step 2: after step 1 is obtained to sample and proportionally mixes with Parasitism broom bacterium conidial powder in high-speed shearing machine, 8000～15000r/min, 20～60min, carries out mist projection granulating immediately after mixing, obtain Parasitism broom bacterium conidial powder dry suspending agent.
Embodiment 8
The present invention provides conidium powder, preparation method thereof simultaneously, and activation culture under 25 DEG C of the bacterial classifications of preservation and 12L:12D condition, to producing spore, is mixed with to 3 × 10 by conidium
8the suspension of individual spore/ml concentration, is inoculated in after blake bottle shaken cultivation 48h (25 ± 1 DEG C, 180r/min), transfers and is inoculation liquid into large-scale blake bottle scale-up medium 24h.Rice (g): water (ml) soaks 20min after mixing by 1: 0.5, cleans that to drain rear moist heat sterilization ripe to the grain of rice sixty percent, admixes inoculation after cooling by 15% inoculation liquid, under the condition of 25 ± 1 DEG C and 12L:12D, cultivates 5～7d.Rice culture is collected to Parasitism broom bacterium conidial powder after vacuum freezedrying is processed, its moisture content is down to below 4%, utilize vibratory sieve that spore and rice matrix are separated, survey its spore content, spore rate alive, put 4 DEG C of conditions for subsequent use.
The screening of described auxiliary agent is carried out mainly for biocompatibility, each auxiliary agent (wetting dispersing agent, uv-protector, disintegrant, binding agent) and carrier is added respectively in nutrient solution in proportion to inoculation 10
6individual spore/ml conidial suspension, 25 ± 1 DEG C, 180r/min shaken cultivation 16h, sampling microscopy germination rate (the spore rate of living).Judge the impact of each auxiliary agent with germination rate height, the spore that germ tube length is equal to or greater than spore diameter is considered as sprouting, with spore fluid suspension in contrast.
The quality standard of described preparation is spore rate >=80.00% (Fig. 1) of living in the outstanding agent of Parasitism broom bacterium conidium dried bean noodles, moisture≤1.63%, pH value is 6.5, spore suspensibility >=82.12%, wetting time is 15s, disintegration time is 78s, 90% granularity < 25 μ m, particle mean size is 10.84 μ m (Fig. 2), 30 DEG C of heat storage stabilities, 60d; For qualified Parasitism broom bacterium dry suspending agent.
Embodiment 9
The application of Parasitism broom bacterium dry suspending agent
1 materials and methods
1.1 for examination material
1.1.1 Botryosphaeria berengeriana f. sp (Physalosporapiricola Nose)
1.1.2 Sclerotinia sclerotiorum (Sclerotinia sclerotiorum)
1.1.3 reagent agent
The dry outstanding agent of present embodiment is 5% Parasitism broom bacterium conidial powder dry suspending agent, 20% Parasitism broom bacterium conidial powder dry suspending agent and 25% Parasitism broom bacterium conidial powder dry suspending agent, and contrast is respectively 70% tpn wetting powder and 25% Fluoxastrobin aqueous suspension agent.
1.2 method
Pot experiment: respectively various pathogens are gone to the dull and stereotyped activation of PDA 3d, then go in PD liquid nutrient medium, every bottle turns 5, cultivate 6d, beat mycelia, survey light transmittance spray inoculation, moisturizing is cultivated cucumber seedling to morbidity, and three kinds of pathogen light transmittances are respectively 1.3%, 3.6% and 9.8%; Parasitism broom bacterium dry suspending agent is taked respectively to dilute 400 times, 800 times and 1600 times of liquid, and spraying control pathogen, checks control efficiency after 15d, according to the sick level index criteria for classifying (table 1), calculate control efficiency according to following formula.
The sick grade index criteria for classifying of table 1
Disease index=(∑ (sick value of series × this disease level number of sheets) × 100)/(sick level peak × investigation number of sheets)
Control efficiency (%)=(CK disease index-processing disease index) × 100/CK disease index
1.3 results and analysis
The isolated test of table 2 Parasitism broom bacterium conidial powder dry suspending agent to ring rot of apple
The isolated test of table 3 Parasitism broom bacterium conidial powder dry suspending agent to rape sclerotium
Adopt the isolated test of the Parasitism broom bacterium conidial powder dry suspending agent of spray technique to the results are shown in Table 2,6.Known, this water dispersible granules to the preventive effect of ring rot of apple more than 75%, to sclerotinia rot of colza preventive effect more than 65%.
The pot experiment of table 4 Parasitism broom bacterium conidial powder dry suspending agent to ring rot of apple, sclerotinia rot of colza
Note: otherness (P=0.01), digital back capitalization in table, represents significance in 99% level.
As shown in Table 4, Parasitism broom bacterium Parasitism broom bacterium conidial powder dry suspending agent is different to the preventive effect of two Plants diseases.Parasitism broom bacterium conidial powder dry suspending agent is to ring rot of apple > sclerotinia rot of colza, though the preventive effect of trial Parasitism broom bacterium conidial powder dry suspending agent is not as good as chemical pesticide Fluoxastrobin, but 400 times of liquid also reach 88% to the preventive effect of ring rot of apple disease, and with not remarkable (P > 0.01) of the Fluoxastrobin preventive effect difference utmost point, though the preventive effect to sclerotinia rot of colza is poorer than chemical pesticide, but also reach more than 75%, and Parasitism broom bacterium conidial powder dry suspending agent microorganism belonging to genus agricultural chemicals category, without the 3R phenomenon of chemical pesticide, it is the agricultural chemicals of environment-friendly and green.
Claims (10)
1. a Parasitism broom bacterium conidium dried bean noodles suspension bactericide; it is characterized in that, it is as follows that formula is pressed mass percent: Parasitism broom bacterium conidial powder 5～30%, wetting dispersing agent 8～20%, uv-protector 0.1～1%, disintegrant 2～20%, binding agent 1.0～10% and carrier 19～83.9%.
2. a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide as claimed in claim 1, is characterized in that: described carrier is a kind of or its composition in attapulgite, kaolin, diatomite, bentonite, white carbon.
3. a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide as claimed in claim 1, is characterized in that: selected wetting dispersing agent is a kind of or its composition in Morwet EFW, k12, MF-5, TERWET1010, WLNO, calcium lignosulfonate, TERSPERSE2700, TERSPERSE2020, Morwet D425, NNO, T36.
4. a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide as claimed in claim 1, is characterized in that: selected disintegrant is a kind of or its composition in crospolyvinylpyrrolidone, Ac-Di-Sol, PVPP, urea, ammonium sulfate, starch.
5. a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide as claimed in claim 1, is characterized in that: selected binding agent is a kind of or its composition in CMC, sodium lignin sulfonate, PEG, oxalic acid, dextrin.
6. a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide as claimed in claim 1, is characterized in that: selected uv-protector is a kind of or its composition of dextrin, xanthans, fluorescein sodium, vitamin C, nano zine oxide.
7. a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide as claimed in claim 1, is characterized in that: described Parasitism broom bacterium conidial powder spore content>=1 × 10
10individual/g.
8. a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide as claimed in claim 1, it is characterized in that, it is as follows that optimization formula is pressed mass percent proportioning: mixture 12%, crospolyvinylpyrrolidone 3%, nano zine oxide 0.2%, PEG4% and the white carbon 75.8% of Parasitism broom bacterium conidial powder 5%, Morwet EFW and calcium lignosulfonate, wherein, Morwet EFW is 1: 5 with the mixture quality ratio of calcium lignosulfonate.
9. a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide as claimed in claim 1, it is characterized in that, it is as follows that optimization formula is pressed mass percent proportioning: mixture 15%, the PVPP 4% of Parasitism broom bacterium conidial powder 20%, TERWET1010 and TERSPERSE2700, vitamin C 0.3%, sodium lignin sulfonate 5% and diatomite 55.7%, wherein the mixture quality of TERWET1010 and TERSPERSE2700 ratio is 1: 4.
10. the application as the disease of the control pathogenic ring rot of apple of agricultural and sclerotinia rot of colza according to a kind of Parasitism broom bacterium conidium dried bean noodles suspension bactericide described in claim 1-9 any one.
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| CN104304328A (en) * | 2014-09-27 | 2015-01-28 | 江苏天象生物科技有限公司 | A preparing method of a hyperparasitic fungal inoculant for sclerotinia sclerotiorum |
| CN105462873A (en) * | 2015-11-17 | 2016-04-06 | 北京克劳沃草业技术开发中心 | Method for making alfalfa rhizobium inoculant and rhizobium inoculant made through same and application thereof |
| CN105519584A (en) * | 2016-01-12 | 2016-04-27 | 郑州曹氏天正生物技术有限公司 | Freeze-dried powdered, dried and suspended fungicide and preparation method thereof |
| CN105707126A (en) * | 2016-03-29 | 2016-06-29 | 陕西先农生物科技有限公司 | Bactericidal composition, preparation and application of bactericidal composition |
| CN105707126B (en) * | 2016-03-29 | 2019-07-05 | 陕西先农生物科技有限公司 | A kind of bactericidal composition, preparation and its application |
| CN115316402A (en) * | 2022-07-29 | 2022-11-11 | 湖北省生物农药工程研究中心 | Trichoderma and dry suspension bactericide and preparation method thereof |
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