CN104014582A - Method for utilizing sorghum bicolor to restore caesium-contaminated soil - Google Patents

Method for utilizing sorghum bicolor to restore caesium-contaminated soil Download PDF

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CN104014582A
CN104014582A CN201410210225.XA CN201410210225A CN104014582A CN 104014582 A CN104014582 A CN 104014582A CN 201410210225 A CN201410210225 A CN 201410210225A CN 104014582 A CN104014582 A CN 104014582A
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caesium
sugar grass
contaminated soil
soil
repair
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CN104014582B (en
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王建龙
王旭
陈灿
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Tsinghua University
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Abstract

The invention discloses a method for utilizing sorghum bicolor to restore caesium-contaminated soil, and belongs to the technical field of environment plant restoration. The method comprises: planting sorghum bicolor in caesium-contaminated soil, and after sorghum bicolor is mature, harvesting the overground part or integrally moving sorghum bicolor away, so as to reach the purpose of restoring caesium-contaminated soil. Energy-plant sorghum bicolor is high in biomass and strong in adaptability, the growth of sorghum bicolor is not influenced by caesium contamination, and the caesium content accumulated at the overground part of sorghum bicolor is higher than the caesium content accumulated at the roots. The method is friendly to environment, simple in operation and technology, convenient in manage, low in cost and good in restoration effect, provides a new choice for effective treatment of radioactive contaminated soil, and is applicable to the field of treatment on heavy-metal contaminated soil.

Description

A kind of method of utilizing sugar grass to repair caesium contaminated soil
Technical field
The present invention relates to the phytoremediation technology field of environment, be specifically related to a kind of method of utilizing crops sugar grass to repair caesium contaminated soil.
Background technology
The extensive use of nuclear technology in many fields such as the energy, scientific research, medical treatment, industry, agricultural, military affairs, traffic, health care brought growing nuclear pollution problem.There is great potential threat to environment and public health in these radwastes.Process these radwastes and become a global difficult problem.Long-life Nuclide Cs-137 in soil ( 137cs) be one of nucleic important in radioactive ash and nuclear fuel waste, 30.17 its half-life were also one of biologically the most dangerous radionuclides.In soil, the migration velocity of caesium is slow, due to similar with potassium of the same clan, the easily food chain that enters absorbed by crops, thus serious harm human health (what is super. the research that radionuclide shifts from soil to organism. and Chinese radiation hygiene, 2007,16 (4): 501-502; Tang Shirong. low biological restoration of putting nucleic pollutant in soil-aqueous medium. Chinese Journal of Applied Ecology, 2002,13 (2): 243-246).
Radionuclide in removing environment is very difficult.For large area low dosage radioactive pollution soil, physical-chemical process is processed low in soil-aqueous medium, and to put nucleic cost high, and easily cause secondary pollution, is difficult to operate on the spot.Phytoremediation technology in bioanalysis is the one method cheaply of administering heavy metal pollution of soil, is also effective, likely, a cheap improvement technology of repairing radionuclide contamination environment.For physical-chemical process recovery technique, phytoremediation technology at present still in the starting stage (Tang Shirong. low biological restoration of putting nucleic pollutant in soil-aqueous medium. Chinese Journal of Applied Ecology, 2002,13 (2): 243-246; Tian Junhua. the phytoremediation of Soil Contaminated with Radionuclides. Sichuan environment, 2007,26 (5): 93-96; Tang Zeping. phytoremediation and utilization that soil radioactivity nucleic pollutes. Agriculture of Anhui science, 2009,37 (13): 6101-6103).
Domesticly aspect radioactive pollution reparation, carrying out lessly, it is also different with the ability of accumulation Cs that different plants absorb.There is at present scholar to disclose and utilize red three-coloured amaranth, grey dish, alternanthera philoxeroides etc. to repair the patent of invention (CN102580992A of low concentration caesium (20-40mg/kg) or high concentration caesium (100-500mg/kg) contaminated soil, CN102580991A, CN101905237A).In the phytoremediation field of contaminated soil, except continuing screening, cultivate and transform super enriching plant (biomass is low, growth is slow, be difficult to meet soil remediation actual requirement), screening is large with plantation biomass, the crops fast that grow clean soil, are another important directions that in soil remediation, newly-developed gets up.
Sugar grass is the non-edible crops of a kind of high yield, paid close attention at energy field, aspect radioactive pollution soil/water pollution control, report is few, has not yet to see the report that utilizes crops sugar grass (Sorghum bicolor L.) to repair tropical monsoons marine climate district radioactive pollution soil.Domestic have utilize false Chinese sorghum (Sorghum haipense) to absorb the report (Huang Renhua of Cs-137 at Mianyang, Sichuan, Lu Yunmei, the impact of yellow bright .AMF on false Chinese sorghum secretion organic acid and absorption Cs-137. nuclear agricultural science report 2013, 27 (8): 1203-1208), there is the report (Wu to Cs-133 accumulation that utilizes sudangrass Hybrid (Sorghum vulgare × Sorghum vulgare var.sudanense hybrids) to carry out in Shenyang, Liaoning, H., Tang, S., Zhang, X., Guo, J., Song, Z., Tian, S., Smith, D.L., 2009.Using elevated CO2to increase the biomass of a Sorghum vulgare x Sorghum vulgare var.sudanense hybrid and Trifolium pratense L.and to trigger hyperaccumulation of cesium.J.Hazard.Mater.170, 861-870.), have and utilize removal news (3 seasons harvesting the remove 45.5%) (Entry of Johnson grass (Sorghum halpense L.Persoon) to Cs-137 in 100 ± 8BqCs-137/kg contaminated soil in greenhouse, J.A., Watrud, L.S., Reeves, M., 2001.Influence of organic amendments on the accumulation of Cs-137and Sr-90from contaminated soil by three grass species.Water Air Soil Pollut.126, 385-398.).
Summary of the invention
The object of the invention is to provides a kind of method of utilizing crops sugar grass to repair caesium contaminated soil for tropical monsoons marine climate district, there is lower cost and good environment friendly, the crop growth that adopts is quick, biomass greatly, ripe, strong stress resistance more than a year.
In order to realize foregoing invention object, technical scheme of the present invention is as follows:
A kind of method of utilizing sugar grass to repair caesium contaminated soil, its method step is: containing planting sweet sorghum and carry out cultivation management in the soil of caesium pollutant, when sugar grass grew to after the maturity period, sugar grass overground part is gathered in or its entirety is removed from contaminated soil.
Containing after planting sweet sorghum on the soil of pollutant caesium, sugar grass root can absorb pollutant caesium from caesium contaminated soil, and is transferred to the aerial part (stem, leaf etc.) of sugar grass.After above-mentioned sugar grass entirety is removed from soil, plant again a collection of sugar grass and carry out cultivation management, when sugar grass grew to after the maturity period, sugar grass aerial part is gathered in or its entirety is removed from contaminated soil, repeat said process, until the caesium content in soil reaches environmental safety standard or thoroughly removes the caesium in contaminated soil.According to local climate, soil regime, sugar grass can be realized ripe more than a year.For example, in Hainan, the ground such as Guangdong, Guangxi utilize its natural conditions can realize 1 year three ripe.
Preferably utilize sugar grass to repair a method for caesium contaminated soil, in tropical monsoons marine climate district, sugar grass is planted in the soil containing caesium pollutant.
The above-mentioned concentration containing caesium in the soil of caesium pollutant is 40-400mg/kg.
The planting patterns of sugar grass is directly sowing or the sugar grass of Seedling Stage is implanted on the soil containing pollutant caesium.
Above-mentioned cultivation management comprises the whole fine earth earth of leveling, waters, applies fertilizer.
Wherein:
In sugar grass cultivation process, according to soil lack of water situation, water, make soil moisture content remain on the 40%-70% of field capacity.
Above-mentioned fertilising refer to containing caesium pollutant soil plant sweet sorghum before basal dressing, and in sugar grass growth course spraying fertilizer.
Above-mentioned base manure is conventional fertilizer with topdressing.Preferred base manure is Eco-fertilizer, and applied amount is 1kg/25kg soil; Preferably topdress and support more than one in root liquid manure (1:1000 solution), urea, compound potassium sulfate fertilizer into marine alga.Impact for the caesium pollutant in soil on sugar grass growth, optionally adds corresponding fertilizer, can make the biomass of sugar grass aerial part increase, thereby improve the recovery rate to caesium in contaminated soil.
Above-mentioned compound potassium sulfate fertilizer is the compound potassium sulfate fertilizer of nitrogenous, phosphorus, the each 15wt% of potassium.
The mass fraction that in above-mentioned Eco-fertilizer, organic content is greater than 35%, N, P, K, Ca, Mg is respectively 3.5%, 1%, 1%, 5% and 1%.
According to sugar grass growing state, can also apply agricultural chemicals, this agricultural chemicals is more than one in AVM, thiophanate-methyl.
Compared with prior art, the present invention has following beneficial effect: the present invention uses sugar grass to repair caesium contaminated soil (40-400mg/kg soil), this energy-source plant not only has advantages of that biomass is high, growth is rapid, growth period is short and strong stress resistance, under 100mg Cs/kg pollution condition, growth does not only normally produce any murder by poisoning phenomenon, when maturity period, the ratio of the caesium content of overground part and root is much larger than 1, there is the super enrichment characteristics of caesium, be suitable for and repair caesium contaminated soil.On the contaminated soil of 400mg/kg caesium, sugar grass growth is subject to impact to a certain extent, but its to the transfer ratio of caesium still much larger than 1, show very strong caesium extractability.Plant restoration method of the present invention operation is simple with technique, disposal cost is low, convenient management, and not spoiled soil basic structure and character, is applicable to the reparation of large-area caesium contaminated soil, for the phytoremediation of caesium contaminated soil provides new selection.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be described in detail, but content not thereby limiting the invention.
Following embodiment adopts outdoor potted plant method to plant sweet sorghum, and the crops sugar grass of choosing is conventional kind, can also adopt other planting patterns and sugar grass kind in application process.
Chemical fertilizer in following embodiment and agricultural chemicals all can be buied by conventional commercial sources.
The absorbability of the each organ of sugar grass to caesium in embodiment 1. pot experiments
Pot experiment place is in farm, Compounds in Dongfang City of Hainan Province.Test site does not have pollution sources around, is the uncontaminated district of heavy metal.Dongfang Shi is positioned at the west and south, Hainan Province, 108 ° 37 of east longitude ' to 109 ° 07 ', 18 ° 43 of north latitude ' to 19 ° 18 ', east tropical monsoons marine climate directly under the jurisdiction of a municipal government, sunshine abundance, 25 DEG C of average temperatures of the whole year.1 18.4 DEG C of monthly mean temperatures, 1.4 DEG C of the extreme lowest temperatures.7 29 DEG C of monthly mean temperatures, Extreme Maximum Temperature is 38.8 DEG C.Approximately 1100 millimeters of average annual rainfalls, average year sunshine time approximately 2500 hours.The soil property of this area's soil is mainly sandy soil, and pot experiment soil used picks up from endosexine, farm, Dongfang Shi soil, and the physicochemical property of soil is as follows:
Table 1 is for examination soil physico-chemical property
Field is carried out radionuclide test and is had very large insecurity in the wild, and due to stable isotope and radio isotope, because of atom proton number identical, belong to a kind of element, except radioactivity difference, the two chemical property is identical, therefore in scientific research, researchers usually use stable isotope to carry out related science test, for example, use caesium-133 ( 133cs) replace 137cs.
Sugar grass adopts flowerpot open-air rearing, investigates sugar grass accumulation to caesium under different caesium levels of pollution.By for experiment soil natural air-dry, smash to pieces, impurities removing, after sieving, stand-by.CsCl is diluted to finite concentration and mixes with a certain amount of soil makes Cs concentration in soil reach 40-400mg/kg.
Meanwhile, in soil, add base manure, can select conventional fertilizer, preferably Eco-fertilizer, the mass fraction that in further preferred Eco-fertilizer, organic content is greater than 35%, N, P, K, Ca, Mg is respectively 3.5%, 1%, 1%, 5% and 1%.After fertilising, stir, pack balance and stability, after 1 month, is put into respectively the flowerpot that model is consistent for subsequent use.1 blank (not adding caesium) is set, and 3 Duplicate Samples are set respectively.
First carry out sugar grass and grow seedlings, the sugar grass of Seedling Stage is transplanted in flowerpot, or sweet sorghum seed is directly sowed, cultivated in flowerpot.Nurturing period, water, spraying fertilizer, apply agricultural chemicals depending on soil moisture content unification, wherein topdress be preferably marine alga support root liquid manure (1:1000 solution), urea, containing a kind of in the potassium sulfate compound fertilizer of N, P, K each 15% or their combination fertilizer., optionally go and buy Chinese medicine according to the growing state of sugar grass meanwhile, use AVM, thiophanate-methyl etc.And make soil moisture content remain on the 40%-70% of field capacity, until sugar grass maturation, sugar grass is ripe needs about 3 months, then harvesting.
The plant that pot experiment is obtained is divided into root, stem, 3 parts of leaf.Stem, leaf are successively clean with running water, deionized water rinsing, cut apart and are placed on 105 DEG C of 15min that complete in baking oven, then at 60-80 DEG C, dry to constant weight, weigh stem, leaf dry weight.Sugar grass root cleans after 2 times with running water, with 20mmol/L EDTA-2Na immersion 15 minutes, then cleans by deionized water, is placed in 105 DEG C, baking oven and completes after 15min, at 60-80 DEG C, dries to constant weight, obtains root dry weight.Stem, leaf, the root of drying used respectively swing, and plasma mass spectrograph is measured content of beary metal wherein, utilizes SPSS software to carry out data statistic analysis.In sugar grass root, stem, leaf, the content of caesium is as shown in table 2.
The uptake (mg/kg) of the each organ of sugar grass to Cs under the different caesium levels of pollution of table 2
Sugar grass is the Cs in enrichment soil preferably, and the Cs in soil can be transferred to plant shoot by root, particularly cane part and leaf part.Sugar grass overground part caesium content, particularly in stem caesium content apparently higher than caesium content in root, be sugar grass to the transfer ratio of caesium much larger than 1, therefore, the suitable plant extraction technology governance caesium contaminated soil utilizing in phytoremediation technology of sugar grass, is removed sugar grass and can be reached the object of rehabilitating soil by cutting aerial part or entirety after the maturity period.
In addition, after sugar grass entirety is removed from contaminated soil, can repeat according to actual needs to plant sweet sorghum and repeatedly repair, until the caesium content in soil reaches environmental safety standard or thoroughly removes the caesium in contaminated soil.
The enrichment characteristics of embodiment 2. sugar grasses to caesium in soil
Plantation, sampling and analyzing step are with embodiment 1.But caesium level of pollution is set to 100mgCs/kg and 400mg Cs/kg, gathers in the crops sugar grass in the time of maturation, cut apart rear acquisition overground part, measure respectively the caesium content calculating in overground part, underground part and soil.
Result shows, more than sugar grass overground part caesium accumulation reaches 758mg/kg, and root is only 336mg/kg, and transfer ratio (ratio of overground part and root caesium content) reaches more than 2.0, much larger than 1.Meanwhile, the caesium concentration of overground part accumulation is higher than caesium concentration in soil, and concentration coefficient (ratio of caesium content in overground part and soil), much larger than 1, reaches 6.4.
Hence one can see that, and when caesium level of pollution is under 100-400mg/kg condition, sugar grass shows higher ability to the accumulation of the pollutant caesium in soil, has very strong Cs transfer, extractability, shows the super enrichment characteristics of caesium.And according to bibliographical information, red three-coloured amaranth is under 13.3~665mg Cs/kg contaminated soil condition, red three-coloured amaranth is approximately 0.17-1.72 (CN101905237A) to the transfer ratio of Cs.Obviously, sugar grass has stronger enriched character to Cs, proves that high yield sugar grass reparation caesium contaminated soil has larger potentiality and advantage than existing plant.
Transfer ratio and the concentration coefficient of table 3 sugar grass to Cs
The upgrowth situation (plant height) of embodiment 3. sugar grass on caesium contaminated soil
By sweet sorghum seed rudiment in nursery, grow seedlings, after growing 3 leaves, select the seedling that growing way is consistent to be transplanted in flowerpot and to cultivate at random, caesium level of pollution is set to 40mgCs/kg, other plantation step as embodiment 1.Measure the height of sugar grass in growth 57 days time, result is as shown in table 4.In 40mg Cs/kg contaminated soil, the growth of sugar grass is substantially unaffected, and its plant height is even higher than contrast sugar grass.
The height (cm) of table 4 sugar grass under 40mg Cs/kg caesium level of pollution
The upgrowth situation (plant height) of embodiment 4. sugar grass on caesium contaminated soil
On the contaminated soil containing 100mg/kg, 400mg/kg caesium, plant sweet sorghum, specifically plant step with embodiment 1.Results in the time that sugar grass is ripe, measure its plant height with chi, and under different caesium levels of pollution, the growing height of sugar grass is in table 5.
Result shows, even on the high concentration caesium contaminated soil of 100mg Cs/kg, sugar grass also can normal growth, and plant height is even higher than control group.In the time that in soil, caesium level of pollution reaches 400mg Cs/kg, sugar grass growth is subject to inhibition to a certain extent, and plant height reduces by 32%.
According to the data of embodiment 3 and embodiment 4, prove that sugar grass has good anti-adversity to caesium contaminated soil, suitable reparation caesium contaminated soil.When caesium level of pollution is during at 40-100mgCs/kg, sugar grass growth is unaffected.Under high pollution condition, sugar grass growth is subject to inhibition to a certain extent, and plant height reduces approximately 1/3.
The growing height (cm) of sugar grass under the different caesium levels of pollution of table 5
The upgrowth situation (biomass) of embodiment 5. sugar grass on caesium contaminated soil
On the contaminated soil containing 100mg/kg, 400mg/kg caesium, plant sweet sorghum, specifically plant step with embodiment 1.In the time that sugar grass is ripe, gather in the crops the overground part of sugar grass, and cut apart the position such as cane, leaf, weigh with scale cane weight in wet base, dry weight etc., concrete outcome is as shown in table 6.
Result shows, when caesium level of pollution is during at 100mg Cs/kg, and sugar grass energy normal growth, and its biomass is higher by 16.6% than the sugar grass in control soil, shows that the caesium pollutant in soil even can promote sugar grass growth but be not remarkable under this level of pollution.Therefore, sugar grass is that 100mg Cs/kg soil still can normal growth when higher in caesium level of pollution, the impact of not contaminated thing caesium.But when Cs is during up to 400mg Cs/kg soil, biomass significantly reduces, it is only 20% of contrast sugar grass biomass.Document
The cane dry weight (g) of sugar grass under the different caesium levels of pollution of table 6
The removal amount of embodiment 6. sugar grasses to caesium
In the contaminated soil containing 100mg/kg and 400mg/kg caesium, 12 sugar grasses of every square metre of plantation, implantation methods is with embodiment 1.Wherein, on 100mg Cs/kg soil, sugar grass is 10.21kg Cs/ha to the removal amount of caesium, is equivalent to 1021mg Cs/m 2; And plant on 400mg Cs/kg contaminated soil, the removal amount of caesium is only 6.95kg Cs/ha, is equivalent to 695mg Cs/m 2.
According to bibliographical information, the removal amount of alternanthera philoxeroides to Cs, in 500mg Cs/kg contaminated soil, approximately removes 533-554mg Cs/m 2; In 300mg Cs/kg soil, the removal amount of caesium is approximately 353-293mg Cs/m 2; In 100mg Cs/kg soil, caesium removal amount is about 115-163mg Cs/m 2(CN102580992A).Grey dish is 100,300, in the contaminated soil of 500mg Cs/kg, and the removal amount of caesium is respectively 202-248,605-706,932-956mg Cs/m 2(CN102580991A).The removing effect of sugar grass unit are to caesium, on 100mg/kg contaminated soil, be better than the achievement of prior art, but on 400mg/kg contaminated soil, although plant height only reduces in 1/3 situation, but its biomass significantly reduces, cause the repairing effect of sugar grass under high concentration Cs pollution condition to decline to some extent, but still be not less than effect of the prior art.
In sum, sugar grass has very strong tolerance to caesium pollutant, and its concentration coefficient and transfer ratio be all better than prior art, has the potentiality of repairing caesium contaminated soil, the reparation of suitable large area low contamination levels caesium contaminated soil.

Claims (10)

1. a method of utilizing crops sugar grass to repair caesium contaminated soil, it is characterized in that, sugar grass is planted in containing in the soil of caesium pollutant and carry out cultivation management, when sugar grass grew to after the maturity period, by the harvesting of sugar grass aerial part or its entirety is removed from contaminated soil.
2. the method for utilizing crops sugar grass to repair caesium contaminated soil according to claim 1, it is characterized in that, after described sugar grass entirety is removed from contaminated soil, plant again a collection of sugar grass and carry out cultivation management, when sugar grass grew to after the maturity period, sugar grass aerial part is gathered in or its entirety is removed from contaminated soil; Repeat said process, until the caesium content in soil reaches environmental safety standard or thoroughly removes the caesium in contaminated soil.
3. the method for utilizing crops sugar grass to repair caesium contaminated soil according to claim 1, is characterized in that, the described concentration containing caesium in the soil of caesium pollutant is 40-400mg/kg.
4. the method for utilizing crops sugar grass to repair caesium contaminated soil according to claim 1 and 2, is characterized in that, in tropical monsoons marine climate district, sugar grass is planted in the soil containing caesium pollutant.
5. the method for utilizing crops sugar grass to repair caesium contaminated soil according to claim 1 and 2, is characterized in that, the planting patterns of sugar grass is directly sowing or the sugar grass of Seedling Stage is implanted on the soil containing pollutant caesium.
6. the method for utilizing crops sugar grass to repair caesium contaminated soil according to claim 1 and 2, is characterized in that, described cultivation management comprises leveling whole fine earth earth, sterilizing, waters and apply fertilizer.
7. the method for utilizing crops sugar grass to repair caesium contaminated soil according to claim 6, is characterized in that, in sugar grass cultivation process, waters according to soil lack of water situation, makes soil moisture content remain on the 40%-70% of field capacity.
8. the method for utilizing crops sugar grass to repair caesium contaminated soil according to claim 6, is characterized in that, described fertilising refers to basal dressing before planting sweet sorghum containing caesium pollutant soil, and in sugar grass growth course spraying fertilizer.
9. the method for utilizing crops sugar grass to repair caesium contaminated soil according to claim 8, is characterized in that, described base manure is Eco-fertilizer; Described topdressing supported more than one in root liquid manure, urea, compound potassium sulfate fertilizer into marine alga.
10. the method for utilizing crops sugar grass to repair caesium contaminated soil according to claim 9, it is characterized in that, the mass fraction that in described Eco-fertilizer, organic content is greater than 35%, N, P, K, Ca, Mg is respectively 3.5%, 1%, 1%, 5% and 1%.
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CN105532227A (en) * 2015-12-21 2016-05-04 新疆农业科学院生物质能源研究所 Sweet-sorghum field planting method for remedying Cd, Pb and Cr compound contaminated soil
CN106269849A (en) * 2016-09-22 2017-01-04 北京新源环境有限公司 A kind of plant bionic and the method for plant combined repairing polluted soil
CN108990728A (en) * 2018-08-20 2018-12-14 湖南省土壤肥料研究所 A kind of cadmium pollution arable land sugar grass surrogate outcomes recovery technique
CN113477704A (en) * 2021-07-30 2021-10-08 西南科技大学 Method for repairing cesium-polluted soil by combination of pasture, pasture and microorganisms

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