CN104012580A - Traditional Chinese medicine preparation for preventing and treating plant downy mildew and preparation method of preparation - Google Patents
Traditional Chinese medicine preparation for preventing and treating plant downy mildew and preparation method of preparation Download PDFInfo
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- CN104012580A CN104012580A CN201410152913.5A CN201410152913A CN104012580A CN 104012580 A CN104012580 A CN 104012580A CN 201410152913 A CN201410152913 A CN 201410152913A CN 104012580 A CN104012580 A CN 104012580A
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Abstract
The invention discloses a traditional Chinese medicine preparation for preventing and treating plant downy mildew and a preparation method of the preparation, and belongs to the field of traditional Chinese medicine prevention of plant downy mildew. The traditional Chinese medicine preparation comprises the following ingredients: fructus cnidii, folium mori, purple perilla, artemisia carvifolia, camellia and liquorice. The invention further discloses a method for preparing the traditional Chinese medicine preparation. The method comprises the following steps: boiling the ingredients in water and mixing the decoction solutions to obtain a decoction agent. The field prevention effect experiment result shows that the traditional Chinese medicine preparation has remarkable prevention and treatment effects on downy mildew of vegetables such as cucumber, tomato and grape or fruit trees, and is particularly very remarkable in prevention effect on downy mildew of greenhouse vegetables.
Description
Technical field
The present invention relates to a kind of pharmaceutical preparation of preventing and treating plant downy mildew, relate in particular to a kind of Chinese medicine preparation of preventing and treating plant downy mildew and preparation method thereof, belong to prevention or the treatment field of plant downy mildew.
Background technology
Downy mildew refers to the common name of disease due to Peronosporaceae pathogen.Peronosporaceae belongs to false Mycota Oomycete Peronosporales, Peronosporaceae has Basidiophora, Sclerospora, refers to Peronospora, Plasmopara, Pseudoperonospora, Peronospora, Bremia and eight genus of Bremiella, mainly causes melon, cabbage, solanaceous vegetables, beans, green onion garlic class, spinach, downy mildew of lettuce.
Cucumis cucurbitaceous plant, is distributed widely in all over China, and is one of main greenhouse product.The downy mildew of cucumber is the disease that a kind of popularity is very strong.Once generation can cause serious economic loss, grave illness plot even complete stool is withered, causes Severe Reduction, even total crop failure.
Downy mildew is one of vegetables Major Diseases, and the downy mildew of vegetables of the same race normally caused by downy mildew of the same race, and they have common symptom characteristic and the identical method of preventing and treating.Downy mildew disease spread and occurrence condition: greenhouse by solar heat, plastic tunnel downy mildew germ survive the winter the summer on sick leaf; Open country downy mildew germ with invalid body in soil, in winter storage kind of strain, survive the winter the summer in seed; Summer, open country downy mildew germ was blown away sporangium from contiguous by monsoon.Sporangium discharges zoospore, and zoospore is sprouted or sporangium direct germination, produces germ tube, invades from host's pore or space between cells, spreads at iuntercellular, stretches absorption nutrition in people's cell by haustorium.Borrow wind and rain to propagate at field sporangium, while having water droplet or moisture film on blade, germ just can be invaded people.Fruit vegetables downy mildew is in 20~24 DEG C of temperature, leaf vegetables downy mildew 16~20 DEG C of temperature, and relative moisture is more than 80%, and especially there are water droplet or moisture film in blade face, has dewfall in canopy, and greenhouse is ventilative bad, and the high humidity duration, long downy mildew was easily popular.
At present, mainly contain following several prophylactico-therapeutic measures for the control of plant downy mildew:
Agronomy prophylactico-therapeutic measures: select disease-resistant variety.Efficent rotation: general equal growing vegetables takes crop rotation more than 4 years.Strengthen cultivation management.
Chemical control: the Agro-chemicals control of control vegetables downy mildew will be to put prevention first.Generally at 1 medicine of premorbid spray.Interval 10d spray 1 time, continuous spraying 2~3 times.Conventional medicament has 500 times of liquid of 58% metalaxyl mancozeb wettable powder, 500 times of liquid of 64% Sandofan wetting powder, 500 times of liquid of 70% mancozeb wettable powder, 600 times of liquid of 48% synergy metalaxyl wetting powder or 600 times of liquid of 72% gram of anti-clever wetting powder.
Chemical control is this sick major measure of current control, but because chemical bactericide uses continuously, the pesticide resistance of germ is more and more stronger, and has to increase formulation rate and spraying times in order to improve control efficiency, causes Pesticide Residue to be on the rise.Organic vegetable can not use any chemical agent, at present plant downy mildew be there is no to effectively preventing medicine, is badly in need of developing to the treatment of this disease and prevention comparatively effectively and to plant and eco-friendly Chinese medicine preparation.
Summary of the invention
Main purpose of the present invention is to provide a kind of Chinese medicine preparation that can effectively prevent or treat plant downy mildew;
Two of object of the present invention is to provide a kind of method of preparing described Chinese medicine preparation.
Above-mentioned purpose of the present invention is mainly achieved through the following technical solutions:
A Chinese medicine preparation for prevention or treatment plant downy mildew, comprises following component:
Frutus cnidii, mulberry leaf, purple perilla, sweet wormwood, camellia and Radix Glycyrrhizae;
Preferably, the weight portion of each component is: frutus cnidii 50-95 part, mulberry leaf 20-45 part, purple perilla 15-35 part, sweet wormwood 5-16 part, camellia 4-15 part, Radix Glycyrrhizae 1-8 part;
Preferred, the weight portion of each component is: frutus cnidii 70-90 part, mulberry leaf 30-40 part, purple perilla 20-30 part, sweet wormwood 8-12 part, camellia 3-10 part, Radix Glycyrrhizae 2-6 part;
Most preferred, the weight portion of each component is: 80 parts of frutus cnidiis, 35 parts, mulberry leaf, 25 parts of purple perillas, 10 parts of sweet wormwoods, 8 parts of camellias, 4 parts, Radix Glycyrrhizae;
The present invention finds by a large amount of experiments, adds the coptis, trifoliate jewelvine and kuh-seng in above-mentioned Chinese medicine preparation, has better Synergistic effect between each medicine, can obtain the effect of better control plant downy mildew; Preferably, the weight portion of the coptis, trifoliate jewelvine and kuh-seng is preferably respectively: coptis 12-25 part, trifoliate jewelvine 20-45 part, kuh-seng 10-35 part; Preferred, 16 parts of the coptiss, 35 parts of trifoliate jewelvines, 25 parts of kuh-sengs.
Frutus cnidii (Cnidium monnieri(L.) Cuss.), bigflower clinopodium herb just by name, another name cicely, is the dry mature fruit of samphire cnidium monnieri.Summer, two season of autumn gather when fruit maturation, remove impurity, dry.Nature and flavor: warm in nature, taste is pungent, bitter.
Mulberry leaf: be the dried leaves of Moraceae Moraceae plant mulberry Morus alba L..Another name: family mulberry, Jing Sang, mulberry fruit tree, yellow wood, red leaves frost, mulberry leaf honey, mulberry leaf, winter folium mori, myriosorus maidenhair herb, family's mulberry leaf, withered mulberry leaf, Jing Sangye, mulberry fruit leaf, mulberry leaves, yellow wood leaf, frosted mulberry leaf, winter folium mori, white mulberry leaf, chicken mulberry leaf, Zisang leaf, morus bombycis leaf, golden mulberry leaf, late mulberry leaf, old mulberry leaf, double leaf, two mulberry leaf, virgin mulberry leaf, Shen Xianye.Nature and flavor: bitter, sweet, cold in nature.
Purple perilla: Labiatae Perilla plant purple perilla Perilla frutescens(L.) the band branch tender leaf of Britt..
Trifoliate jewelvine: another name, malicious trifoliate jewelvine, pulse family China tree Calamus plant trifoliate jewelvine Derris trifoliata Lour., is used as medicine with complete stool and root-like stock.Can adopt the whole year, cleans chopping, dries.Meridian distribution of property and flavor: pungent, temperature.Very toxic.
Kuh-seng (Radix Sophorae flavescentis): be the plant of pulse family kuh-seng genus.Bitter; Cold in nature.Function cures mainly: heat-clearing and damp-drying drug, desinsection, diuresis.
Sweet wormwood: be the dry aerial parts of feverfew sweet wormwood Artemisia annua L..Clearing away summerheat, except steaming, preventing malaria.For summer-heat evil heating, fever due to yin deficiency, night fever abating at dawn, hectic fever due to yin labor heat, malaria fever and chills, jaundice with damp-heat pathogen.
Camellia: be Theaceae Camellia Plants camellia Camellia japonica L., be used as medicine with root, flower.Root can be adopted the whole year; Flower Chun Dongcai, dries or dries.Taste is sweet and micro-pungent, and gas is put down and is slightly cold.Restrain cool blood, hemostasis.For spitting blood, bleeding from five sense organs or subcutaneous tissue, has blood in stool, metrorrhagia; Burn and scald is controlled in external application, traumatic bleeding
The coptis: for ranunculaceae plant coptis Coptis chinensis Franch. triangle leaf coptis Coptis deltoidea C.Y.Chenget Hsiao or cloud connect the dry rhizome of Coptis teeta Wall.; There is heat-clearing and damp-drying drug, effect of purging intense heat and detonicating.Full for damp and hot ruffian, vomiting acid regurgitation, rushes down dysentery, jaundice, and unconsciousness due to high fever, heart-fire hyperactivity, dysphoria and insomnia, blood-head is told nosebleed, hot eyes, toothache, quenches one's thirst, the carbuncle furunculosis that swells; Control eczema outward, wet sore, duct is suppurated.
Another object of the present invention is to provide a kind of method of the Chinese medicine preparation of preparing described prevention or treatment plant downy mildew, and the method comprises: each bulk drug water is decocted, merge decoction liquor, obtain decoction agent.
Wherein, preferred, each bulk drug is boiled 1-3 time with the decocting of 3-8 times of weight.
The present invention also provides Chinese medicine preparation of the present invention for preventing or treat the method for plant downy mildew, the method comprises: by decoction agent dilute with water of the present invention, as for the degree of dilution, the visual concrete condition of those skilled in the art is determined, as a reference, can be watered dilution 10-50 doubly by decocting agent; Liquid after dilution is sprayed onto uniformly to the positive and negative of leaf.
Field efficacy experimental result shows, Chinese medicine composition of the present invention has significant prevention and result for the treatment of, the particularly preventive effect that has highly significant of the downy mildew of the vegetables in booth for the downy mildew of the plants such as cucumber, tomato, grape.
Embodiment
By the following example, embodiments of the present invention will be more specifically described, it should be understood that described embodiment is only exemplary, does not form any restriction to scope of the present invention.It will be understood by those skilled in the art that lower without departing from the spirit and scope of the present invention and can the details of technical solution of the present invention and form be modified or be replaced, but these amendments or replacement all fall into protection scope of the present invention.
Embodiment 1 decocts the preparation of agent
Take each raw material (unit: kg) by described weight: frutus cnidii 5, mulberry leaf 2, purple perilla 1.5, sweet wormwood 0.5, camellia 0.4, Radix Glycyrrhizae 0.1; Each bulk drug is boiled 3 times with the decocting of 3 times of weight, merge decoction liquor, obtain decoction agent.
Embodiment 2 decocts the preparation of agent
Take each raw material (unit: kg) by described weight: frutus cnidii 9.5, mulberry leaf 4.5, purple perilla 3.5, sweet wormwood 1.6, camellia 1.5, Radix Glycyrrhizae 0.8; Each bulk drug is boiled 2 times with the decocting of 8 times of weight, merge decoction liquor, obtain decoction agent.
Embodiment 3 decocts the preparation of agent
Take each raw material (unit: kg) by described weight: frutus cnidii 8, mulberry leaf 3.5, purple perilla 2.5, sweet wormwood 1.0, camellia 0.8, Radix Glycyrrhizae 0.4; Each bulk drug is boiled 2 times with the decocting of 5 times of weight, merge decoction liquor, obtain decoction agent.
Embodiment 4 decocts the preparation of agent
Take each raw material (unit: kg) by described weight: frutus cnidii 5.0, mulberry leaf 2.0, purple perilla 1.5, sweet wormwood 0.5, camellia 0.4, Radix Glycyrrhizae 0.1, the coptis 1.2, trifoliate jewelvine 2.0, kuh-seng 1.0; Each bulk drug is boiled 3 times with the decocting of 3 times of weight, merge decoction liquor, obtain decoction agent.
Embodiment 5 decocts the preparation of agent
Take each raw material (unit: kg) by described weight: frutus cnidii 9.5, mulberry leaf 4.5, purple perilla 3.5, sweet wormwood 1.6, camellia 1.5, Radix Glycyrrhizae 0.8, the coptis 2.5, trifoliate jewelvine 4.5, kuh-seng 3.5; Each bulk drug is boiled 2 times with the decocting of 8 times of weight, merge decoction liquor, obtain decoction agent.
Embodiment 6 decocts the preparation of agent
Take each raw material (unit: kg) by described weight: frutus cnidii 8, mulberry leaf 3.5, purple perilla 2.5, sweet wormwood 1.0, camellia 0.8, Radix Glycyrrhizae 0.4, the coptis 1.6, trifoliate jewelvine 3.5, kuh-seng 2.5; Each bulk drug is boiled 2 times with the decocting of 5 times of weight, merge decoction liquor, obtain decoction agent.
The field efficacy experiment of experimental example 1 Chinese medicine composition of the present invention to cucumber downy mildew
One, reagent agent
The decoction agent that embodiment 1-6 is prepared.
Two, experimental technique
Reagent agent is mixed with respectively to the medicament that concentration is 50mg/L, the contrast taking 500mg/L metalaxyl as standard medicament, clear water is blank, each processing repeats 3 times.In the time that growing 2-3 sheet leaf, the cucumber in booth tests.
A, prevention effect determination test: decoct agent and be watered dilution 10-50 doubly; Liquid after dilution is sprayed onto to the positive and negative drop method dispenser of leaf uniformly, after moisturizing 24h, then meets bacterium moisturizing 24h with drop method, then proceed to Routine Management, after clear water contrast is fully fallen ill, investigation prevention effect.
B. therapeutic action determination test.Method and prevention effect are basic identical, and difference is first to connect bacterium, dispenser again after moisturizing 24h.Stage division and computing formula: 0 grade, blade is without scab; 1 grade, lesion area accounts for whole blade area below 5%; 3 grades, lesion area accounts for whole blade area 6%~10%; 5 grades, lesion area accounts for whole blade area 11%~25%; 7 grades, lesion area accounts for whole blade area 26%~50%; 9 grades, lesion area accounts for the more than 50% of whole blade area.
Disease index=(sick grade blade number × this disease value of series of Σ)/(investigating total number of sheets × the highest sick value of series);
Relative control effect (%)=" (check plot disease index one treatment region index)/check plot disease index] x100%.
Data analysis: carry out data statistics and analysis with Excel2003, carry out variance analysis with SAS8.I.
Three, experimental result
Experimental result is in table 1.
Table 1 Chinese medicine composition of the present invention is for the field efficacy experimental result of cucumber downy mildew
From experimental result, Chinese medicine composition of the present invention all has certain prevention and therapeutic efficiency for cucumber downy mildew, and wherein the relative control effect of the prepared pharmaceutical preparation of embodiment 4-6 will be significantly higher than the relative control effect of embodiment 1-3 pharmaceutical preparation to cucumber downy mildew.
The field efficacy experiment of experimental example 2 Chinese medicine preparation of the present invention to downy mildew of garpe
One, reagent agent
The decoction agent that embodiment 1-6 is prepared.
Two, experimental technique
Reagent agent is mixed with respectively to the medicament that concentration is 60mg/L, the contrast taking 500mg/L metalaxyl as standard medicament, clear water is blank, each processing repeats 3 times.In the time that growing 2-3 sheet leaf, tests grape.
A, prevention effect test: decoct agent and be watered dilution 10-50 doubly; Liquid after dilution is sprayed onto to the positive and negative drop method dispenser of grape leaf uniformly, after moisturizing 24h, then meets bacterium moisturizing 24h with drop method, then proceed to Routine Management, after clear water contrast is fully fallen ill, investigation prevention effect.
B. therapeutic action test: method and prevention effect are basic identical, difference is first to connect bacterium, dispenser again after moisturizing 24h.
Stage division and computing formula: 0 grade, blade is without scab; 1 grade, lesion area accounts for whole blade area below 5%; 3 grades, lesion area accounts for whole blade area 6%~10%; 5 grades, lesion area accounts for whole blade area 11%~25%; 7 grades, lesion area accounts for whole blade area 26%~50%; 9 grades, lesion area accounts for the more than 50% of whole blade area.
Disease index=(sick grade blade number × this disease value of series of Σ)/(investigating total number of sheets × the highest sick value of series);
Relative control effect (%)=" (check plot disease index one treatment region index)/check plot disease index] x100%.
Data analysis: carry out data statistics and analysis with Excel2003, carry out variance analysis with SAS8.I.
Three, experimental result
Experimental result is in table 2.
Table 2 Chinese medicine composition of the present invention is for the field efficacy experimental result of downy mildew of garpe
From experimental result, Chinese medicine preparation of the present invention all has prevention and therapeutic efficiency for downy mildew of garpe, and wherein the relative control effect of the prepared pharmaceutical preparation of embodiment 4-6 will be significantly higher than the relative control effect of embodiment 1-3 pharmaceutical preparation to downy mildew of garpe.
Claims (10)
1. a Chinese medicine preparation for prevention or treatment plant downy mildew, is characterized in that, comprises following component: frutus cnidii, mulberry leaf, purple perilla, sweet wormwood, camellia and Radix Glycyrrhizae.
2. according to Chinese medicine preparation claimed in claim 1, it is characterized in that, the weight portion of each component is: frutus cnidii 50-95 part, mulberry leaf 20-45 part, purple perilla 15-35 part, sweet wormwood 5-16 part, camellia 4-15 part, Radix Glycyrrhizae 1-8 part.
3. according to Chinese medicine preparation claimed in claim 2, it is characterized in that, the weight portion of each component is: frutus cnidii 70-90 part, mulberry leaf 30-40 part, purple perilla 20-30 part, sweet wormwood 8-12 part, camellia 3-10 part, Radix Glycyrrhizae 2-6 part.
4. according to Chinese medicine preparation claimed in claim 3, it is characterized in that, the weight portion of each component is: 80 parts of frutus cnidiis, 35 parts, mulberry leaf, 25 parts of purple perillas, 10 parts of sweet wormwoods, 8 parts of camellias, 4 parts, Radix Glycyrrhizae.
5. a Chinese medicine preparation for prevention or treatment plant downy mildew, is characterized in that, comprises following component: frutus cnidii, mulberry leaf, purple perilla, sweet wormwood, camellia, Radix Glycyrrhizae, the coptis, trifoliate jewelvine and kuh-seng.
6. according to Chinese medicine preparation claimed in claim 5, it is characterized in that, the weight portion of each component is: frutus cnidii 50-95 part, mulberry leaf 20-45 part, purple perilla 15-35 part, sweet wormwood 5-16 part, camellia 4-15 part, Radix Glycyrrhizae 1-8 part, coptis 12-25 part, trifoliate jewelvine 20-45 part, kuh-seng 10-35 part.
7. according to Chinese medicine preparation claimed in claim 6, it is characterized in that, the weight portion of each component is: 80 parts of frutus cnidiis, 35 parts, mulberry leaf, 25 parts of purple perillas, 10 parts of sweet wormwoods, 8 parts of camellias, 4 parts, Radix Glycyrrhizae, 16 parts of the coptiss, 35 parts of trifoliate jewelvines, 25 parts of kuh-sengs.
8. a method of preparing claim 1-7 Chinese medicine preparation described in any one, the method comprises: each component water is decocted, merge decoction liquor, obtain decoction agent.
9. claim 1-7 Chinese medicine preparation application in prevention or treatment plant downy mildew described in any one.
10. according to application claimed in claim 9, it is characterized in that: described plant comprises vegetables or fruit tree; Preferably, described vegetables comprise cucumber, tomato or lettuce; Described fruit tree is grape.
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Cited By (7)
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CN104322576A (en) * | 2014-10-13 | 2015-02-04 | 句容市满园春家庭农场 | Grape downy mildew prevention and treatment medicine |
CN104355808A (en) * | 2014-10-29 | 2015-02-18 | 苏州科大微龙信息技术有限公司 | Lettuce downy mildew preventing and treating leaf fertilizer |
CN104872210A (en) * | 2015-04-15 | 2015-09-02 | 陈凯 | Medicine for preventing and treating lettuce downy mildew |
CN105010442A (en) * | 2015-07-09 | 2015-11-04 | 襄汾县科农种植专业合作社 | Biological pesticide for grape planting |
CN105010436A (en) * | 2015-06-26 | 2015-11-04 | 安徽禾众农业科技有限公司 | Grape downy mildew prevention and control bacteriostat, and preparation method and application thereof |
CN105941493A (en) * | 2016-06-02 | 2016-09-21 | 江苏丰年果品种植园有限公司 | Bactericide for preventing and treating blueberry stiff fruit disease and preparation method thereof |
CN109526928A (en) * | 2018-11-21 | 2019-03-29 | 临沂丰邦植物医院有限公司 | A kind of Organic cucumber downy mildew preventing control method |
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CN104322576A (en) * | 2014-10-13 | 2015-02-04 | 句容市满园春家庭农场 | Grape downy mildew prevention and treatment medicine |
CN104355808A (en) * | 2014-10-29 | 2015-02-18 | 苏州科大微龙信息技术有限公司 | Lettuce downy mildew preventing and treating leaf fertilizer |
CN104872210A (en) * | 2015-04-15 | 2015-09-02 | 陈凯 | Medicine for preventing and treating lettuce downy mildew |
CN105010436A (en) * | 2015-06-26 | 2015-11-04 | 安徽禾众农业科技有限公司 | Grape downy mildew prevention and control bacteriostat, and preparation method and application thereof |
CN105010442A (en) * | 2015-07-09 | 2015-11-04 | 襄汾县科农种植专业合作社 | Biological pesticide for grape planting |
CN105941493A (en) * | 2016-06-02 | 2016-09-21 | 江苏丰年果品种植园有限公司 | Bactericide for preventing and treating blueberry stiff fruit disease and preparation method thereof |
CN109526928A (en) * | 2018-11-21 | 2019-03-29 | 临沂丰邦植物医院有限公司 | A kind of Organic cucumber downy mildew preventing control method |
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