CN104012395A - Device and method for raising seedlings of taxus chinensis by cutting branches - Google Patents
Device and method for raising seedlings of taxus chinensis by cutting branches Download PDFInfo
- Publication number
- CN104012395A CN104012395A CN201410283688.9A CN201410283688A CN104012395A CN 104012395 A CN104012395 A CN 104012395A CN 201410283688 A CN201410283688 A CN 201410283688A CN 104012395 A CN104012395 A CN 104012395A
- Authority
- CN
- China
- Prior art keywords
- container
- chinese yew
- cavity volume
- branches
- culture fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a device and a method for raising seedlings of taxus chinensis by cutting branches. The device comprises a light-transmitting container, wherein the middle part of the container is provided with a sealed separation plate; a siphon pipe, a communication pipe and an infusion pipe are respectively connected between upper and lower containing cavities. The upper containing cavity is internally provided with an air nozzle and a branch positioning net. The method for raising the seedlings of the taxus chinensis by cutting the branches comprises the following steps: cutting fresh taxus chinensis tender branches and inserting the tender branches into the upper containing cavity of the container; positioning by using the branch positioning net; opening an infusion pump to transfer a culture solution to the upper containing cavity from the lower containing cavity of the container and ventilating by utilizing the air nozzle; and circularly transferring the culture solution between the upper and lower parts of the container in a reciprocated manner under the effect of the siphon pipe so that the taxus chinensis branches grow into small tree seedlings under the condition of alternatively immersing the taxus chinensis branches into a liquid and exposing. The device provided by the invention is made of a light-transmitting material; a whole rooting process can be observed; in a cutting process, a sterile condition is not needed and the culture solution is used for replacing nutrient soil; the operation is convenient and the survival rate is high; the device and the method have wide popularization and application value.
Description
Technical field
The present invention relates to is plant cultivation technology, specifically a kind of device and method of Chinese yew branch cuttage and seedling culture.
Background technology
Chinese yew is first-grade state protection plant, because receiving much concern containing anticancer active constituent taxol.The relevant anticancer injection of taxol has sale at various big hospital at present, and determined curative effect is expensive.But content of taxol is very low in Chinese yew, be only generally the 0.005%-0.07% of dry weight, and existing taxus resource is limited, can not meet the demand in market far away.Because growth of taxol is slow, self-reproduction degree is low; Artificial synthetic method cost is high, and there is no ripe technology path; From the bark of Chinese yew, be separated to the fungi that some can produce taxol, but it is too low to be separated at present the output of fungi, there is no the value of fermenting and producing.
The artificial cuttage and seedling culture of Chinese yew branch establishing in large scale are effective ways that solves shortage of resources.Yet traditional field cottage method survival rate is low, a large amount of cuttage branches, because not surviving and be abandoned, have caused the serious waste of Chinese yew resource.
Summary of the invention
Object of the present invention, exactly in order to address the above problem, provides a kind of Chinese yew branch cuttage and seedling culture device and method.
In order to achieve the above object, the present invention has adopted following technical scheme: a kind of Chinese yew branch cuttage and seedling culture apparatus and method, this device comprises light transmission container, in the middle of container, be provided with seal diaphragm container is separated into upper and lower two cavity volumes, between upper and lower two cavity volumes, be connected with respectively a siphon pipe, a communicating pipe and a woven hose, woven hose is provided with infusion pump; In the cavity volume of top, be provided with gas blowout head and branch network for location.
Suitable for reading and the environmental communication of described container.
Distance≤1mm between described siphonal suction inlet and seal diaphragm.
Described gas blowout head is arranged on branch network for location below and approaches seal diaphragm place.
Chinese yew branch cuttage and seedling culture method based on Chinese yew branch cuttage and seedling culture device is: culture fluid is injected in the bottom cavity volume of container, fresh Chinese yew epicormic branch cuttage is also fixed with branch network for location in the top cavity volume of container; Opening infusion pump transfers to culture fluid top cavity volume and by gas blowout head, ventilates simultaneously from the bottom cavity volume of container, when the liquid level of the liquid in the cavity volume of top surpasses siphonal peak, culture fluid is transferred to rapidly bottom cavity volume under siphonage, so, culture fluid reciprocation cycle between container upper and lower shifts, and makes Chinese yew branch grow into little sapling under soaked with liquid and the exposed condition alternately occurring.
Described culture fluid is not sugary 1/10MS+IBA0.1-1.0mg/L culture fluid.
The described culture fluid cycle that reciprocation cycle shifts between container upper and lower is 25-35 minute.
Device of the present invention adopts light transmissive material to make, and whole rooting process can be observed; In addition, do not need aseptic condition during cuttage, with culture fluid, replace Nutrition Soil, easy to operate, survival rate is high, has application value.
Accompanying drawing explanation
Fig. 1 is the structural representation of the Chinese yew branch cuttage and seedling culture device in the present invention.
Embodiment
Referring to Fig. 1, Chinese yew branch cuttage and seedling culture device in the present invention, comprise light transmission container 1, in the middle of container, be provided with seal diaphragm 2 container is separated into upper and lower two cavity volumes 11,12, between upper and lower two cavity volumes, be connected with respectively a siphon pipe 3, a communicating pipe 4 and a woven hose 5, woven hose is provided with infusion pump 6; In the cavity volume of top, be provided with gas blowout 7 and branch network for location 8.Wherein, the suitable for reading and environmental communication of container 1.The suction inlet of siphon pipe 3 and the distance≤1mm between seal diaphragm.Gas blowout head is arranged on branch network for location below and approaches seal diaphragm place.
The Chinese yew branch cuttage and seedling culture method that the present invention is based on Chinese yew branch cuttage and seedling culture device is: culture fluid is injected in the bottom cavity volume of container, fresh Chinese yew epicormic branch cuttage is also fixed with branch network for location in the top cavity volume of container; Opening infusion pump transfers to culture fluid top cavity volume and by gas blowout head, ventilates simultaneously from the bottom cavity volume of container, when the liquid level of the liquid in the cavity volume of top surpasses siphonal peak, culture fluid is transferred to rapidly bottom cavity volume under siphonage, so, culture fluid reciprocation cycle between container upper and lower shifts, and makes Chinese yew branch grow into little sapling under soaked with liquid and the exposed condition alternately occurring.Culture fluid is wherein not sugary 1/10MS+IBA0.1-1.0mg/L culture fluid.The culture fluid cycle that reciprocation cycle shifts between container upper and lower is 25-35 minute.
It is below a specific embodiment.
Volume of a container is 10L, and Chinese yew kind used is southern enqlish yew, taxus chinensis in northeast, Xizang Taxus chinensis, taxusyunnanensis and taxus media.Culture fluid formula is: not sugary 1/10MS+IBA1.0mg/L culture fluid.Culture fluid is 30 minutes at the upper and lower two-part migration period of container, cultivates after 55-65 days, and 5 kinds of Chinese yew branches are all taken root, and rooting rate reaches 100%.
Claims (7)
1. a Chinese yew branch cuttage and seedling culture device, it is characterized in that: comprise light transmission container, in the middle of container, be provided with seal diaphragm container is separated into upper and lower two cavity volumes, between upper and lower two cavity volumes, be connected with respectively a siphon pipe, a communicating pipe and a woven hose, woven hose is provided with infusion pump; In the cavity volume of top, be provided with gas blowout head and branch network for location.
2. Chinese yew branch cuttage and seedling culture device as claimed in claim 1, is characterized in that: the suitable for reading and environmental communication of described container.
3. Chinese yew branch cuttage and seedling culture device as claimed in claim 1, is characterized in that: the distance≤1mm between described siphonal suction inlet and seal diaphragm.
4. Chinese yew branch cuttage and seedling culture device as claimed in claim 1, is characterized in that: described gas blowout head is arranged on branch network for location below and approaches seal diaphragm place.
5. the Chinese yew branch cuttage and seedling culture method based on Chinese yew branch cuttage and seedling culture device, is characterized in that: culture fluid is injected in the bottom cavity volume of container, fresh Chinese yew epicormic branch cuttage is also fixed with branch network for location in the top cavity volume of container; Opening infusion pump transfers to culture fluid top cavity volume and by gas blowout head, ventilates simultaneously from the bottom cavity volume of container, when the liquid level of the liquid in the cavity volume of top surpasses siphonal peak, culture fluid is transferred to rapidly bottom cavity volume under siphonage, so, culture fluid reciprocation cycle between container upper and lower shifts, and makes Chinese yew branch grow into little sapling under soaked with liquid and the exposed condition alternately occurring.
6. Chinese yew branch cuttage and seedling culture method as claimed in claim 5, is characterized in that: described culture fluid is not sugary 1/10MS+IBA0.1-1.0mg/L culture fluid.
7. Chinese yew branch cuttage and seedling culture method as claimed in claim 5, is characterized in that: the described culture fluid cycle that reciprocation cycle shifts between container upper and lower is 25-35 minute.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410283688.9A CN104012395B (en) | 2014-06-23 | 2014-06-23 | Chinese yew branch cuttage and seedling culture device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410283688.9A CN104012395B (en) | 2014-06-23 | 2014-06-23 | Chinese yew branch cuttage and seedling culture device and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104012395A true CN104012395A (en) | 2014-09-03 |
| CN104012395B CN104012395B (en) | 2016-02-10 |
Family
ID=51429626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410283688.9A Expired - Fee Related CN104012395B (en) | 2014-06-23 | 2014-06-23 | Chinese yew branch cuttage and seedling culture device and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104012395B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920025A (en) * | 2015-06-04 | 2015-09-23 | 湖北天健农产品有限公司 | Cutting breeding method |
| CN108605537A (en) * | 2018-05-08 | 2018-10-02 | 李明 | A kind of method of purplefruit holly leaf epicormic branch cutting |
| CN110622731A (en) * | 2019-09-26 | 2019-12-31 | 陕西睿方农业科技集团有限公司 | High-survival-rate fig cutting seedling device and seedling method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6410931A (en) * | 1986-11-10 | 1989-01-13 | Kunio Date | Siphon aquiculture tank |
| CN2323582Y (en) * | 1997-11-12 | 1999-06-16 | 朱果利 | Siphon device for hydroponics |
| JP2007259815A (en) * | 2006-03-29 | 2007-10-11 | Chugoku Electric Power Co Inc:The | Vertically positioned hydroponic cultivation device |
| CN201355972Y (en) * | 2009-01-25 | 2009-12-09 | 张宗旺 | Nursery box of red date trees |
| CN102687660A (en) * | 2011-12-02 | 2012-09-26 | 河南科技大学 | Soilless cultivation device |
| CN202635271U (en) * | 2012-02-13 | 2013-01-02 | 中国林业科学研究院林业研究所 | Pipeline type micro-mist cuttage culture apparatus |
| CN103031251A (en) * | 2012-12-07 | 2013-04-10 | 上海交通大学 | Multifunctional plant excised root culture bioreactor system |
-
2014
- 2014-06-23 CN CN201410283688.9A patent/CN104012395B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6410931A (en) * | 1986-11-10 | 1989-01-13 | Kunio Date | Siphon aquiculture tank |
| CN2323582Y (en) * | 1997-11-12 | 1999-06-16 | 朱果利 | Siphon device for hydroponics |
| JP2007259815A (en) * | 2006-03-29 | 2007-10-11 | Chugoku Electric Power Co Inc:The | Vertically positioned hydroponic cultivation device |
| CN201355972Y (en) * | 2009-01-25 | 2009-12-09 | 张宗旺 | Nursery box of red date trees |
| CN102687660A (en) * | 2011-12-02 | 2012-09-26 | 河南科技大学 | Soilless cultivation device |
| CN202635271U (en) * | 2012-02-13 | 2013-01-02 | 中国林业科学研究院林业研究所 | Pipeline type micro-mist cuttage culture apparatus |
| CN103031251A (en) * | 2012-12-07 | 2013-04-10 | 上海交通大学 | Multifunctional plant excised root culture bioreactor system |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920025A (en) * | 2015-06-04 | 2015-09-23 | 湖北天健农产品有限公司 | Cutting breeding method |
| CN104920025B (en) * | 2015-06-04 | 2018-05-11 | 湖北天健农产品有限公司 | Cottage breeding method |
| CN108605537A (en) * | 2018-05-08 | 2018-10-02 | 李明 | A kind of method of purplefruit holly leaf epicormic branch cutting |
| CN110622731A (en) * | 2019-09-26 | 2019-12-31 | 陕西睿方农业科技集团有限公司 | High-survival-rate fig cutting seedling device and seedling method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104012395B (en) | 2016-02-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103704105B (en) | A kind of officinal dendrobium stem plantation dedicated substrate and application thereof | |
| CN103891620A (en) | Taxus chinensis var mairei cultivation method | |
| CN204560501U (en) | A kind of novel plant water culture experiment device | |
| CN104823824A (en) | Temporary immersion type culture method and culture device for dendrobe | |
| CN104012395B (en) | Chinese yew branch cuttage and seedling culture device and method | |
| CN103548660B (en) | Plant seedling pot allowing automatic liquid absorption | |
| CN103081745B (en) | Pteridophyte seedling raising device | |
| CN204146134U (en) | Compound water planting groove | |
| CN108283141A (en) | A kind of solid superposing type depth runner trickle irrigation cultivation apparatus without soil | |
| CN204191406U (en) | A kind of pink rice borer artificial feeding bottle | |
| CN108541487B (en) | Device and method suitable for mycorrhizal seedling cultivation under open air condition | |
| CN203194207U (en) | Soil culture, soilless culture and water culture plant planting device | |
| CN204070049U (en) | Multifunctional water culture device | |
| CN102499085B (en) | Method for raising directly transplantable seedlings of dendrobium candidum | |
| CN108713449A (en) | A kind of cultural method of Poria cocos | |
| CN108541514B (en) | Method for cultivating orange Rumex Lactarius mycorrhiza seedlings | |
| TWI417381B (en) | Cactus camellia fruiting body and mycelia fast culture flask device | |
| CN205922169U (en) | Breeding device of gastrodia elata | |
| CN220274326U (en) | Forestry cuttage equipment of growing seedlings | |
| CN204443478U (en) | Sapling plantation irrigator | |
| CN203261943U (en) | Plant soilless cultivation sterilization purification automatic water injection device | |
| CN203387967U (en) | Seabed planting case | |
| CN107711517A (en) | A kind of candidum tissue culturing seedling breeding method of high-survival rate | |
| CN101653093A (en) | Method for culturing water culture flowers | |
| CN218570988U (en) | A seedling raising box for nandina is cultivated |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160210 Termination date: 20180623 |