CN104003796B - A kind of cordyceps militaris plantation wheat grain culture medium - Google Patents
A kind of cordyceps militaris plantation wheat grain culture medium Download PDFInfo
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- CN104003796B CN104003796B CN201410187182.8A CN201410187182A CN104003796B CN 104003796 B CN104003796 B CN 104003796B CN 201410187182 A CN201410187182 A CN 201410187182A CN 104003796 B CN104003796 B CN 104003796B
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Abstract
A kind of cordyceps militaris plantation wheat grain culture medium, relates to Cordyceps militaris (L.) Link. culture technique field, and including culture matrix and nutritional solution, the mass ratio of described culture matrix and described nutritional solution is 1:2; Described culture matrix includes wheat grain 400 parts, 100 parts of Semen Viciae fabae mud; Described nutritional solution is formed by the preparation of raw material of following weight portion: peptone 9 parts, glucose 35 parts, potassium dihydrogen phosphate 4 parts, 2 parts of magnesium sulfate, 850 parts of water, Sucus Oenanthes Javanicae 50 parts, Radix Dauci Sativae juice 30 parts, maltose 20 parts; The pH value of nutritional solution is 6.5. The Chinese caterpillar fungus culture medium of the present invention can be greatly improved the content of the cordycepin in Cordyceps militaris (L.) Link. and cordycepic acid so that it is medicinal function and health care are higher, it is possible to promotes growth and the breeding of Cordyceps militaris (L.) Link. flora.
Description
Technical field
The present invention relates to Cordyceps militaris (L.) Link. culture technique field, particularly relate to a kind of cordyceps militaris plantation wheat grain culture medium.
Background technology
Cordyceps militaris (L.) Link. worldwide distribution natural resources quantity is little. By Stroma (i.e. grass part) and sclerotium (i.e. the corpse part of worm) complex dimerous. Winter, larva lived in seclusion in soil, and mushroom parasitism wherein, draws nutrition, was full of mycelia in larva body and dead. To summer, on larva corpse, bear seedling, likeness in form grass, gather before and after the Summer Solstice and obtain. Cordyceps militaris (L.) Link. has edible and medical value.
The domestic research to cordyceps militaris plantation and culture medium is more traditional at present. Causing that the cordycepin content in Cordyceps militaris (L.) Link. is typically in about 4000mg/kg, cordycepic acid content is only about 12%. And the part of most critical in the nutrient substance of cordycepin and cordycepic acid Cordyceps militaris (L.) Link. just. Cordycepin is nucleoside antibiotics, and cordycepic acid is a kind of D type mannitol. Improve the content of both materials in Cordyceps militaris (L.) Link., can be greatly promoted Cordyceps militaris (L.) Link. to insomnia, rheumatism, rheumatoid disease, cardiovascular disease alleviation and auxiliary therapeutic action.
Summary of the invention
The technical problem to be solved is in that to overcome the defect of prior art, it is further proposed that a kind of cordyceps militaris plantation wheat grain culture medium.
The technical problem to be solved realizes by the following technical solutions.
A kind of cordyceps militaris plantation wheat grain culture medium, it is characterised in that including culture matrix and nutritional solution, the mass ratio of described culture matrix and described nutritional solution is 1:2;
Described culture matrix includes wheat grain 400 parts, 100 parts of Semen Viciae fabae mud;
Described nutritional solution is formed by the preparation of raw material of following weight portion: peptone 9 parts, glucose 35 parts, potassium dihydrogen phosphate 4 parts, 2 parts of magnesium sulfate, 850 parts of water, Sucus Oenanthes Javanicae 50 parts, Radix Dauci Sativae juice 30 parts, maltose 20 parts; The pH value of nutritional solution is 6.5;
The preparation method of wheat grain culture medium:
Preparing nutritional solution after being mixed in proportion by each material component preparing nutritional solution, clean Semen Viciae fabae to smash to pieces after boiling and make Semen Viciae fabae mud, the Semen Viciae fabae mud taking component amount is mixed and made into culture matrix with wheat grain; Nutritional solution is added in culture matrix, prepare the wheat grain culture medium of cultivation Cordyceps militaris (L.) Link.;
Cordyceps militaris plantation method is:
Being inoculated in seed culture medium by Cordyceps militaris (L.) Link., it is thus achieved that the seed liquor of Cordyceps militaris (L.) Link., then seed liquor be inoculated in wheat grain culture medium, in the seed liquor that every 100g wheat grain culture medium is inoculated, (with mycelium pellet weight in wet base meter) containing mycelia is for 2g;
After inoculation, wheat grain culture medium being placed in constant temperature indoor cultivation, under dark condition, indoor temperature controls at 18～20 DEG C, is cultured to described cordyceps mycelium and is full of culture medium;
Then Cordyceps militaris (L.) Link. carries out illumination cultivation, and every day, light application time was, 12 hours, and temperature is 23 DEG C, all the other times be 16 DEG C dark when be cultured to the former base of the Stroma of described Cordyceps militaris (L.) Link. and formed;
Afterwards under 20～25 DEG C of conditions, illumination every day 10 hours, all the other times are the sporophore being cultured under dark condition and obtaining described Cordyceps militaris (L.) Link..
Further preferred version,
In described culture matrix, also included raw material components is expanded Semen Glycines powder, expanded Semen Glycines powder and Semen Viciae fabae mud equivalent.
The preparation method of described expanded Semen Glycines powder is: is first soaked in water by Semen sojae atricolor 2～5 hours, pulls dewatering when fold occurs in Semen sojae atricolor epidermis out, grinds to form Semen Glycines powder after dewatering, makes Semen Glycines powder surface air-dry, is then added by Semen Glycines powder in extruser again and carries out expanding treatment. Semen Glycines powder is after expanding treatment, and nutrient substance is easier to be utilized by mushroom.
The pH value of the water in the nutritional solution of the present invention is 7.4, and iodide containing the zinc of 0.03～0.1mg/L, the free carbon dioxide of 10～12mg/L, the calcium of 60～70mg/L and 0.1～0.2mg/L in water.
The invention have the benefit that
The present invention is fully studying Cordyceps militaris (L.) Link. growth characteristic under natural environment and strain habit, and selected a kind of high quality combination formula. The present invention adopts maltose and glucose combined effect, and Cordyceps militaris (L.) Link. mushroom, owing to liking growth and the breeding of sugar energy strong impulse Cordyceps militaris (L.) Link. flora, is greatly improved the Cordyceps polysaccharide content in Cordyceps militaris (L.) Link.; Glucose can directly be utilized by mushroom, and maltose can be decomposed utilization for a long time by maltose.
The addition of green vegetable juice can promote growth and the breeding of Cordyceps militaris (L.) Link. flora, provides necessary vegetable protein and trace element for the breeding of Cordyceps militaris (L.) Link. flora growth.
The feature of the Cordyceps militaris (L.) Link. cultivated according to the Chinese caterpillar fungus culture medium of the present invention is: survival rate is to 98%, and cordycepin content reaches more than 8500mg/kg, and cordycepic acid content is more than 18%.
The Chinese caterpillar fungus culture medium of the present invention can be greatly improved the content of the cordycepin in Cordyceps militaris (L.) Link. and cordycepic acid so that it is medicinal function and health care are higher.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and effect and be easy to understand, below in conjunction with specific embodiment, the present invention is expanded on further.
Embodiment 1
A kind of cordyceps militaris plantation wheat grain culture medium, including culture matrix and nutritional solution, the mass ratio of described culture matrix and described nutritional solution is 1:2;
Described culture matrix includes wheat grain 400g, Semen Viciae fabae mud 100g;
Described nutritional solution is formed by the preparation of raw material of following weight g: peptone 9g, glucose 35g, potassium dihydrogen phosphate 4g, magnesium sulfate 2g, water 850g, Sucus Oenanthes Javanicae 50g, Radix Dauci Sativae juice 30g, maltose 20g; The pH value of nutritional solution is 6.5;
The preparation method of wheat grain culture medium:
Preparing nutritional solution after being mixed in proportion by each material component preparing nutritional solution, clean Semen Viciae fabae to smash to pieces after boiling and make Semen Viciae fabae mud, the Semen Viciae fabae mud taking component amount is mixed and made into culture matrix with wheat grain; Nutritional solution is added in culture matrix, prepare the wheat grain culture medium of cultivation Cordyceps militaris (L.) Link.;
Cordyceps militaris plantation method is:
Being inoculated in seed culture medium by Cordyceps militaris (L.) Link., it is thus achieved that the seed liquor of Cordyceps militaris (L.) Link., then seed liquor be inoculated in wheat grain culture medium, in the seed liquor that every 100g wheat grain culture medium is inoculated, (with mycelium pellet weight in wet base meter) containing mycelia is for 2g;
After inoculation, wheat grain culture medium being placed in constant temperature indoor cultivation, under dark condition, indoor temperature controls at 18～20 DEG C, is cultured to described cordyceps mycelium and is full of culture medium;
Then Cordyceps militaris (L.) Link. carries out illumination cultivation, and every day, light application time was, 12 hours, and temperature is 23 DEG C, all the other times be 16 DEG C dark when be cultured to the former base of the Stroma of described Cordyceps militaris (L.) Link. and formed;
Afterwards under 20～25 DEG C of conditions, illumination every day 10 hours, all the other times are the sporophore being cultured under dark condition and obtaining described Cordyceps militaris (L.) Link..
Embodiment 2
A kind of cordyceps militaris plantation wheat grain culture medium, including culture matrix and nutritional solution, the mass ratio of described culture matrix and described nutritional solution is 1:2;
Described culture matrix includes wheat grain 400g, Semen Viciae fabae mud 100g, expanded Semen Glycines powder 100g;
Described nutritional solution is formed by the preparation of raw material of following weight: peptone 9g, glucose 35g, potassium dihydrogen phosphate 4g, magnesium sulfate 2g, water 850g, Sucus Oenanthes Javanicae 50g, Radix Dauci Sativae juice 30g, maltose 20g; The pH value of nutritional solution is 6.5;
The preparation method of wheat grain culture medium:
Preparing nutritional solution after being mixed in proportion by each material component preparing nutritional solution, clean Semen Viciae fabae to smash to pieces after boiling and make Semen Viciae fabae mud, the Semen Viciae fabae mud taking component amount is mixed and made into culture matrix with wheat grain; Nutritional solution is added in culture matrix, prepare the wheat grain culture medium of cultivation Cordyceps militaris (L.) Link.;
Cordyceps militaris plantation method is:
Being inoculated in seed culture medium by Cordyceps militaris (L.) Link., it is thus achieved that the seed liquor of Cordyceps militaris (L.) Link., then seed liquor be inoculated in wheat grain culture medium, in the seed liquor that every 100g wheat grain culture medium is inoculated, (with mycelium pellet weight in wet base meter) containing mycelia is for 2g;
After inoculation, wheat grain culture medium being placed in constant temperature indoor cultivation, under dark condition, indoor temperature controls at 18～20 DEG C, is cultured to described cordyceps mycelium and is full of culture medium;
Then Cordyceps militaris (L.) Link. carries out illumination cultivation, and every day, light application time was, 12 hours, and temperature is 23 DEG C, all the other times be 16 DEG C dark when be cultured to the former base of the Stroma of described Cordyceps militaris (L.) Link. and formed;
Afterwards under 20～25 DEG C of conditions, illumination every day 10 hours, all the other times are the sporophore being cultured under dark condition and obtaining described Cordyceps militaris (L.) Link..
The ultimate principle of the present invention, principal character and advantages of the present invention have more than been shown and described. Skilled person will appreciate that of the industry; the present invention is not restricted to the described embodiments; described in above-described embodiment and description is that principles of the invention is described; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements both fall within the claimed scope of the invention. Claimed scope is defined by appending claims and equivalent thereof.
Claims (3)
1. a cordyceps militaris plantation wheat grain culture medium, it is characterised in that include culture matrix and nutritional solution, the mass ratio of described culture matrix and described nutritional solution is 1:2;
Described culture matrix includes wheat grain 400 parts, 100 parts of Semen Viciae fabae mud;
Described nutritional solution is formed by the preparation of raw material of following weight portion: peptone 9 parts, glucose 35 parts, potassium dihydrogen phosphate 4 parts, 2 parts of magnesium sulfate, 850 parts of water, Sucus Oenanthes Javanicae 50 parts, Radix Dauci Sativae juice 30 parts, maltose 20 parts; The pH value of nutritional solution is 6.5;
The preparation method of wheat grain culture medium:
Preparing nutritional solution after being mixed in proportion by each material component preparing nutritional solution, clean Semen Viciae fabae to smash to pieces after boiling and make Semen Viciae fabae mud, the Semen Viciae fabae mud taking component amount is mixed and made into culture matrix with wheat grain;Nutritional solution is added in culture matrix, prepare the wheat grain culture medium of cultivation Cordyceps militaris (L.) Link.;
Cordyceps militaris plantation method is:
Cordyceps militaris (L.) Link. is inoculated in seed culture medium, it is thus achieved that the seed liquor of Cordyceps militaris (L.) Link., then seed liquor is inoculated in wheat grain culture medium, every 100g wheat grain culture medium is 2g containing mycelia in the seed liquor of inoculation, in mycelium pellet weight in wet base;
After inoculation, wheat grain culture medium being placed in constant temperature indoor cultivation, under dark condition, indoor temperature controls at 18～20 DEG C, is cultured to described cordyceps mycelium and is full of culture medium;
Then Cordyceps militaris (L.) Link. carries out illumination cultivation, and every day, light application time was 12 hours, and temperature is 23 DEG C, all the other times be 16 DEG C dark when be cultured to the former base of the Stroma of described Cordyceps militaris (L.) Link. and formed;
Afterwards under 20～25 DEG C of conditions, illumination every day 10 hours, all the other times are the sporophore being cultured under dark condition and obtaining described Cordyceps militaris (L.) Link.;
The pH value of the water in described nutritional solution is 7.4, and iodide containing the zinc of 0.03～0.1mg/L, the free carbon dioxide of 10～12mg/L, the calcium of 60～70mg/L and 0.1～0.2mg/L in water.
2. cordyceps militaris plantation wheat grain culture medium according to claim 1, it is characterised in that: in described culture matrix, also included raw material components is expanded Semen Glycines powder.
3. cordyceps militaris plantation wheat grain culture medium according to claim 2, it is characterized in that, the preparation method of described expanded Semen Glycines powder is: be first soaked in water by Semen sojae atricolor 2～5 hours, dewatering is pulled out when fold occurs in Semen sojae atricolor epidermis, Semen Glycines powder is ground to form after dewatering, make Semen Glycines powder surface air-dry, then Semen Glycines powder is added in extruser again and carry out expanding treatment.
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| CN104718985A (en) * | 2015-03-20 | 2015-06-24 | 江南大学 | Cordyceps sinensis rice |
| CN107347439A (en) * | 2017-07-07 | 2017-11-17 | 上海市农业科学院 | A kind of production method of meat rod bacteria cultivation strain |
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| JPH07170856A (en) * | 1993-10-26 | 1995-07-11 | Kirin Brewery Co Ltd | Culture bed for culturing mushroom and method for culturing mushroom |
| CN101173218B (en) * | 2007-09-30 | 2010-07-21 | 袁有宝 | Cultivate method for cordyceps militaris link |
| CN101440389A (en) * | 2008-10-23 | 2009-05-27 | 贵州大学 | Method for increasing cordycepin content in Cordceps militaris solid culture medium |
| CN102100152B (en) * | 2010-11-17 | 2012-05-23 | 鲁东大学 | Artificial culture method and culture medium for fruiting bodies of cordyceps militaris |
| CN103503689B (en) * | 2012-06-18 | 2016-01-20 | 北京仙源食品酿造有限公司 | A kind of method and medium thereof utilizing bean dregs cultivation Cordyceps militaris |
| CN103553755B (en) * | 2013-11-13 | 2015-05-27 | 青海洲龙生物科技有限责任公司 | Cordyceps sinensis bacterial powder and production method thereof as well as special culture medium |
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