CN107347439A - A kind of production method of meat rod bacteria cultivation strain - Google Patents
A kind of production method of meat rod bacteria cultivation strain Download PDFInfo
- Publication number
- CN107347439A CN107347439A CN201710550013.XA CN201710550013A CN107347439A CN 107347439 A CN107347439 A CN 107347439A CN 201710550013 A CN201710550013 A CN 201710550013A CN 107347439 A CN107347439 A CN 107347439A
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- China
- Prior art keywords
- meat rod
- wheat
- production method
- bacteria cultivation
- meat
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of production method of meat rod bacteria cultivation strain, including：(1) wheat culture matrix manufacturing；(2) sterilize；(3) it is inoculated with；(4) cultivate.The present invention is simple to operate, available for the cultivation of potential new resource food meat rod bacterium, has started development and the utilization beginning of the potential new resource food meat rod bacterium in China.
Description
Technical field
The invention belongs to edible and medical fungi field, more particularly to a kind of production method of meat rod bacteria cultivation strain.
Background technology
China is one of most abundant country of bio-diversity, and China is estimated to be 100,000 kinds of edible and medical fungis, has been realized
Only 1000 kinds or so, and only 100 kinds or so be utilized, it can be seen that most of wild edible and medical fungis are undiscovered, recognize
Know and utilize.Therefore it is one of important process of China edible and medical fungi worker to find more preferable edible and medical fungi.
Meat rod bacterium (Podostroma sp.) is that one kind has 16 son specialized species ascosporous, and the world is on the books at present
Meat rod Pseudomonas have 20 records, it can be seen that meat rod bacterium treasures degree.Meat rod bacterium is not recognized also in China by vast majority of people
Know and understand, or even most people for being engaged in fungus research is also without the concept of this species, and China's only several kinds of quilts at present
Description and record, do not have also isolate and purify the report that strain and cultivation etc. utilize so far.According to Primary Study, it is special that meat rod bacterium has
Edible and medical value, therefore how research isolates and purifies meat rod bacterium strain, so as to carry out corresponding developmental research, is
Develop edible and medical fungi new varieties, the important channel of developing new drug thing.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of production method of meat rod bacteria cultivation strain, this method operation
Simply, the cultivation available for potential new resource food meat rod bacterium, started the potential new resource food meat rod bacterium in China development and
Develop the beginning.
A kind of production method of meat rod bacteria cultivation strain of the present invention, including：
(1) choose full seed, without broken grain, the wheat without moth, dried, added with being taken out after pure water immersion 12-24h
0.5~1% quick lime and 0.5~1% gypsum (percentage is on the basis of wheat quality), are filled after being stirred with the wheat dried
Enter in container, then add nutrient solution, adjust pH to 7, obtain wheat culture medium, it is subject to sterilization；
(2) by 121 DEG C of -123 DEG C of autoclaving 90-120min of wheat culture medium, normal temperature is cooled to after taking-up, it is to be seeded；
(3) advance cultured meat rod bacterium original seed 3-5 blocks are taken, it is scattered to be inoculated in the wheat medium container for bacterium of having gone out；
30-45d is cultivated under 20 DEG C of -25 DEG C of half-lights and produces meat rod bacterium kernel culture.
Nutrient solution in the step (1) is 1% peptone lysate, 1% beef extract lysate and 1%KH2PO4Dissolving
The mixed liquor of liquid.
The ratio of wheat and nutrient solution in the step (1) is 15g:20ml.
Meat rod bacterium original seed in the step (3) is cultivated in advance in 20-25 DEG C of room temperature environment.
A diameter of 7-8mm of meat rod bacterium original seed in the step (3).
Beneficial effect
The present invention is simple to operate, available for the cultivation of potential new resource food meat rod bacterium, has started the potential new resources in China
The development of food meat rod bacterium and the utilization beginning.
Brief description of the drawings
Fig. 1 is meat rod bacterium kernel culture photo.
Embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.In addition, it is to be understood that after the content of the invention lectured has been read, people in the art
Member can make various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited
Scope.
Embodiment 1
(1) wheat culture matrix manufacturing：Full seed is chosen, without broken grain, the wheat without moth, after pure water immersion 12h
Taking-up is dried, and adds 0.5% quick lime and 0.5% gypsum (percentage is on the basis of wheat quality), is stirred with the wheat dried
It is fitted into after mixing thoroughly in vial, per bottled half bottle or so of the wheat mixed thoroughly, then every bottle adds 20 milliliters of nutrient solution (1% eggs
White peptone lysate, 1% beef extract lysate, 1% KH2PO4Lysate mixed liquor), pH to 7 is adjusted, it is subject to sterilization；
(2) sterilize：The wheat culture medium being fitted into vial is sterilized, 121 DEG C of -123 DEG C of autoclaving 90-
120min, superclean bench is put into after taking-up and is cooled to normal temperature, it is to be seeded；
(3) it is inoculated with：Cultured meat rod bacterium original seed 3-5 blocks (diameter 7-8mm) are taken, the scattered wheat training for being inoculated in bacterium of having gone out
Support in base vial；
(4) cultivate：30-45d is cultivated under 20-25 DEG C of half-light and obtains meat rod bacterium kernel culture, as shown in Figure 1.
Claims (5)
1. a kind of production method of meat rod bacteria cultivation strain, including：
(1) choose full seed, without broken grain, the wheat without moth, dried with being taken out after pure water immersion 12-24h, add 0.5
~1% quick lime and 0.5~1% gypsum, are fitted into container after being stirred with the wheat dried, then add nutrient solution, adjust
PH to 7 is saved, obtains wheat culture medium, it is subject to sterilization；
(2) by 121 DEG C of -123 DEG C of autoclaving 90-120min of wheat culture medium, normal temperature is cooled to after taking-up, it is to be seeded；
(3) advance cultured meat rod bacterium original seed 3-5 blocks are taken, it is scattered to be inoculated in the wheat medium container for bacterium of having gone out；20
Culture 30-45d produces meat rod bacterium kernel culture under DEG C -25 DEG C of half-lights.
A kind of 2. production method of meat rod bacteria cultivation strain according to claim 1, it is characterised in that：The step (1)
In nutrient solution be 1% peptone lysate, 1% beef extract lysate and 1%KH2PO4The mixed liquor of lysate.
A kind of 3. production method of meat rod bacteria cultivation strain according to claim 1, it is characterised in that：The step (1)
In wheat and the ratio of nutrient solution be 15g:20ml.
A kind of 4. production method of meat rod bacteria cultivation strain according to claim 1, it is characterised in that：The step (3)
In meat rod bacterium original seed cultivated in advance in 20-25 DEG C of room temperature environment.
A kind of 5. production method of meat rod bacteria cultivation strain according to claim 1, it is characterised in that：The step (3)
In meat rod bacterium original seed a diameter of 7-8mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710550013.XA CN107347439A (en) | 2017-07-07 | 2017-07-07 | A kind of production method of meat rod bacteria cultivation strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710550013.XA CN107347439A (en) | 2017-07-07 | 2017-07-07 | A kind of production method of meat rod bacteria cultivation strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107347439A true CN107347439A (en) | 2017-11-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710550013.XA Pending CN107347439A (en) | 2017-07-07 | 2017-07-07 | A kind of production method of meat rod bacteria cultivation strain |
Country Status (1)
| Country | Link |
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| CN (1) | CN107347439A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104003796A (en) * | 2014-05-05 | 2014-08-27 | 安徽金豪生态农业科技有限公司 | Wheat grain medium for cultivation of cordyceps militaris |
| CN104272978A (en) * | 2014-10-30 | 2015-01-14 | 湖南省宇秀生物科技有限公司 | Pleurotus eryngii solid strain liquefaction process |
-
2017
- 2017-07-07 CN CN201710550013.XA patent/CN107347439A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104003796A (en) * | 2014-05-05 | 2014-08-27 | 安徽金豪生态农业科技有限公司 | Wheat grain medium for cultivation of cordyceps militaris |
| CN104272978A (en) * | 2014-10-30 | 2015-01-14 | 湖南省宇秀生物科技有限公司 | Pleurotus eryngii solid strain liquefaction process |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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Application publication date: 20171117 |