CN104000196A - Compound peony seed oil preparation, and making method and application thereof - Google Patents
Compound peony seed oil preparation, and making method and application thereof Download PDFInfo
- Publication number
- CN104000196A CN104000196A CN201410249654.8A CN201410249654A CN104000196A CN 104000196 A CN104000196 A CN 104000196A CN 201410249654 A CN201410249654 A CN 201410249654A CN 104000196 A CN104000196 A CN 104000196A
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- CN
- China
- Prior art keywords
- seed oil
- peony seed
- tree peony
- compound tree
- oil formulation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000009286 beneficial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000025084 cell cycle arrest Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 210000003989 endothelium vascular Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- YLTGFGDODHXMFB-UHFFFAOYSA-N isoacetovanillon Natural products COC1=CC=C(C(C)=O)C=C1O YLTGFGDODHXMFB-UHFFFAOYSA-N 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- 239000012669 liquid formulation Substances 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- SYSQUGFVNFXIIT-UHFFFAOYSA-N n-[4-(1,3-benzoxazol-2-yl)phenyl]-4-nitrobenzenesulfonamide Chemical class C1=CC([N+](=O)[O-])=CC=C1S(=O)(=O)NC1=CC=C(C=2OC3=CC=CC=C3N=2)C=C1 SYSQUGFVNFXIIT-UHFFFAOYSA-N 0.000 description 1
- 230000000324 neuroprotective effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- MLIBGOFSXXWRIY-UHFFFAOYSA-N paeonol Natural products COC1=CC=C(O)C(C(C)=O)=C1 MLIBGOFSXXWRIY-UHFFFAOYSA-N 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 244000144985 peep Species 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000008165 rice bran oil Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 229940001083 tocotrienols 50 mg Drugs 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
- 239000008802 xuezhikang Substances 0.000 description 1
- RZFHLOLGZPDCHJ-XZXLULOTSA-N α-Tocotrienol Chemical compound OC1=C(C)C(C)=C2O[C@@](CC/C=C(C)/CC/C=C(C)/CCC=C(C)C)(C)CCC2=C1C RZFHLOLGZPDCHJ-XZXLULOTSA-N 0.000 description 1
- 235000019145 α-tocotrienol Nutrition 0.000 description 1
- 239000011730 α-tocotrienol Substances 0.000 description 1
- 235000019151 β-tocotrienol Nutrition 0.000 description 1
- 150000003782 β-tocotrienols Chemical class 0.000 description 1
- 235000019150 γ-tocotrienol Nutrition 0.000 description 1
- 150000003786 γ-tocotrienols Chemical class 0.000 description 1
- 235000019144 δ-tocotrienol Nutrition 0.000 description 1
- 150000003790 δ-tocotrienols Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Medicinal Preparation (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a method for making a compound peony seed oil preparation from peony seed oil, tocotrienols and red rice, the compound peony seed oil preparation, and application of the compound peony seed oil preparation. The compound peony seed oil preparation can be in the form of liquid or powder, or can be further processed to be in the form of dissolvable granules, tablets, capsules, oral liquid or the like, has the characteristics of safety, high efficiency, biological utilization degree, stable quality and industrialization, and has the outstanding effects of reducing the blood fat, the blood pressure and the cholesterol, alleviating cardiovascular and cerebrovascular diseases, resisting tumors and resisting fatigue.
Description
Technical field
The invention belongs to health medicine field of food, be specifically related to the application of a kind of compound tree peony seed oil formulation and preparation method thereof, this compound tree peony seed oil formulation.
Background technology
Natural VE is the compound of natural tocopherol, comprises tocopherol and tocotrienols, is the fat-soluble vitamin of a class.Tocotrienols comprises alpha-tocotrienol, β-tocotrienols, γ-tocotrienols and 4 kinds of isomers of δ-tocotrienols, tocotrienols is mainly present in the minority cereal such as barley, wheat, and especially in rice bran oil, palm oil, wheat bran, Arnotto seed, content is more.The structural main distinction of tocotrienols and tocopherol is; tocotrienols has unsaturated terminal chain; this makes the oxidation resistance of tocotrienols stronger; approximately 40～60 times of tocopherol, and have reduce cholesterol, anticancer, protect unique physiological function such as cardiovascular and neuroprotective.Tocotrienols, by inhibition tumor cell propagation, inducing apoptosis of tumour cell, induced tumor cell cycle arrest, inhibition HMG-CoA reductase activity, the new Angiogenesis of inhibition tumour, reduction telomerase activation etc., is brought into play the physiological function that it is better than tocopherol.
Red colouring agent for food, also used as a Chinese medicine, claims red song ancient times, with monascus fungi, is inoculated on rice and is prepared from through fermentation.Red colouring agent for food, also used as a Chinese medicine is as traditional Chinese medicine, and Ming Dynasty's Li Shizhen (1518-1593 A.D.) is evaluated in < < Compendium of Materia Medica > >: " help digestion and invigorate blood circulation, the dry stomach of invigorating the spleen; Sweet, warm, nontoxic; This be people peep good fortune skilful person also! ".Modern study confirms, red colouring agent for food, also used as a Chinese medicine has the multiple efficacies such as reducing blood lipid, hypotensive, hypoglycemic, antitumor, antifatigue.Red colouring agent for food, also used as a Chinese medicine can suppress atherosclerotic development, improves function of vascular endothelium, reduces the generation of adhesion molecule.Modern Chinese herbal medicine Effects of Xuezhikang and the Zhibituo with China's independent intellectual property right, main component is red colouring agent for food, also used as a Chinese medicine.
Peony seed oil is approved as new resource food in the 2011 Nian Bei Ministry of Public Health, through the PONY of internal authority testing agency, detect, the contained unrighted acid of peony seed oil is up to more than 92%, wherein alpha-linolenic acid accounts for 42%, linoleic acid accounts for 28%, and oleic acid accounts for 22%, also containing tens kinds of bioactivators such as paeonol, tree peony saponins, nutritive value is very abundant, is a kind of fabulous natural health care source.Yet, unrighted acid in peony seed oil, very easily be subject to the impact of the physics such as ambient light photograph, air, temperature, metal ion and chemical factor and change, produce the chemical substances such as peroxide, hydroxide, aldehyde, ketone, low-molecular-weight fatty acid, trans-fatty acid, not only reduce nutritive value of food and digestive utilization ratio, and the material producing is harmful to health.
Summary of the invention
The preparation that the object of this invention is to provide a kind of compound tree peony seed oil, and preparation method and related application.
The invention provides a kind of compound tree peony seed oil formulation, it is characterized in that, described compound tree peony seed oil formulation comprises peony seed oil and tocotrienols, and preferred, described preparation further comprises red colouring agent for food, also used as a Chinese medicine.
Preferably, described preparation is liquid compound tree peony seed oil formulation, or by microcapsules technology, will be dissolved with the peony seed oil parcel of tocotrienols or tocotrienols and red colouring agent for food, also used as a Chinese medicine, the Powdered compound tree peony seed oil formulation being prepared into.
Further, described compound tree peony seed oil formulation, includes following component by weight: tocotrienols 0.1%～60%, peony seed oil 40%～99.9%.Or tocotrienols 0.1%～50%, red colouring agent for food, also used as a Chinese medicine 1%～20%, peony seed oil 30%～98.9%.
The present invention also provides a kind of preparation method of compound tree peony seed oil formulation, it is characterized in that, the peony seed oil of proper proportion is heated to 50～80 ℃, the tocotrienols or tocotrienols and the red colouring agent for food, also used as a Chinese medicine that add aforementioned proportion, stir, homogeneous preparation becomes the liquid compound tree peony seed oil formulation of stable homogeneous.
Further, the present invention also provides a kind of preparation method of Powdered compound tree peony seed oil formulation, described method comprises that take above-mentioned liquid compound tree peony seed oil formulation is core, and one of following component is wall material, by microcapsules technology, prepares Powdered compound tree peony seed oil formulation:
(1) yeast;
(2) soybean protein isolate and sodium alginate;
(3) tree peony cake cake protein and sodium alginate; Or
(4) one or more in cyclodextrin, maltodextrin, starch octenyl succinate anhydride, shitosan, lactose, xanthans, guar gum, Arabic gum.
Preferred version (1), described wall material is yeast, further, described yeast is the waste yeast liquid of wine brewing or antibiotics plant, or food grade dry ferment.
Preferably, described method comprises the steps (1) yeast purifying or deactivation, (2) yeast autolysis, (3) packing.
Preferably, in described packing step, core: wall material mass ratio is 1: 2～3: 1.
Preferably, described yeast purifying or inactivation step, for getting wine brewing or antibiotics plant waste yeast liquid, by the sterile water wash of 2～3 times of volumes, are sieved respectively 2 times with 80 orders, 100 object sieves, remove hop resin and other larger impurity, under 3000～5000r/min normal temperature, centrifugal 5～10min, abandons supernatant, then uses sterile water wash 1～2 time, to supernatant colorless and odorless, centrifugally obtain milky yeast milk, dry through vacuum drying or spraying, make dry ferment.The food grade dry ferment of living, 65～70 ℃ of deactivation 20～30min, or ultra violet lamp deactivation.
Preferably, described yeast autolysis step is the dry ferment of getting after purifying or deactivation, add the water of 4～10 times to make suspension (being yeast: water is 1: 4～1: 10), add again autolysis promoter ethanol 0.5～5%, and to adjust Initial pH be 5～7, changing temperature autolysis 12～48h within the scope of 40～60 ℃, at 80～95 ℃, enzyme 10～the 15min that goes out, after 3000～5000r/min centrifugation, 30～40min, it is standby that 30～50 ℃ of vacuum drying or freeze drying obtain yeast cell wall.
Preferably, described packing step is core for take liquid compound tree peony seed oil formulation, yeast after processing is wall material, in 1: 2～3: 1 ratios of core wall material mass ratio, add liquid compound tree peony seed oil formulation and yeast sealing, put into 50～80 ℃ of water bath with thermostatic control oscillators, frequency of oscillation 80～160r/min, vibration 6～12h, liquid compound tree peony seed oil formulation can enter yeast cells inside by diffusion effect, form microcapsules, vacuum filtration reclaims the liquid compound tree peony seed oil formulation not wrapping into, use again 2～3 yeast surface raffinates of absolute ethanol washing, 30～50 ℃ of vacuum drying 20～30min or freeze drying, obtain Powdered compound tree peony seed oil formulation.
Preferred version (2), described wall material is soybean protein isolate and sodium alginate, further, the mass ratio of described soybean protein isolate and sodium alginate is 2: 1～5: 1.
Preferably, described method comprises that take liquid compound tree peony seed oil formulation is core, and soybean protein isolate and sodium alginate are wall material, through following steps (1) emulsification, (2) packing, (3) solidify, dry preparation and obtaining.
Preferably, described core and wall material mass ratio are 1: 2～2: 1.
Preferably, described emulsifying step is soybean protein isolate and distilled water to mix for 1: 20 in mass ratio～1: 30, add 60～80 ℃ of stirring and dissolving of certain emulsifying agent, with alkali regulator solution pH value to 7～9, make solution and sodium alginate there is identical electric charge.
Preferably, described emulsifying agent is one or more of citric acid monoglyceride, sucrose ester, lecithin, soybean lecithin, glycerine monofatty ester, and emulsifying agent addition is 0.1～0.3% of microcapsules gross mass.
Preferably, described packing step is 40～80 ℃ of temperature, add a certain proportion of liquid compound tree peony seed oil formulation, homogeneous 5～the 10min of 3000～6000r/min high speed forms the emulsion of homogeneous, with acid for adjusting pH value to 3～4 (isoelectric point of soybean protein isolate is 4.1), make soybean protein isolate and sodium alginate with opposite charges, and stirring at low speed 20～25min, the multiple condensed phase that wall material generation complex coacervation produces is wrapped in liquid compound tree peony seed oil formulation surface, form microcapsule suspensions, be diluted with water and be placed in ice bath and continue to be stirred to below 10 ℃, make the gelation of gained complex coacervation thing.
Preferably, described curing, drying steps, is to add curing agent, and stirring at low speed is solidified 1～2h, and stratification is collected the capsule that wets after filtering, washing, and through 30～50 ℃ of vacuum freeze dryings, obtains Powdered compound tree peony seed oil formulation.
Preferably, described curing agent is one or more of glucono-δ-lactone, glucose, pectin, tannic acid, and the addition of curing agent is 0.8～2% of microcapsules gross mass.
Preferred version (3), described wall material is tree peony cake cake protein and sodium alginate, further, the mass ratio of described tree peony cake cake protein and sodium alginate is 2: 1～5: 1.
Preferably, described method comprises that take liquid compound tree peony seed oil formulation is core, and tree peony cake cake protein and sodium alginate are wall material, through following steps (1) emulsification, (2) packing, (3) solidify, dry preparation and obtaining.
Preferably, described core and wall material mass ratio are 1: 2～2: 1.
Preferably, described emulsifying step is tree peony cake cake protein and distilled water to mix for 1: 20 in mass ratio～1: 30, add 60～80 ℃ of stirring and dissolving of certain emulsifying agent, with alkali regulator solution pH value to 7～9, make solution and sodium alginate there is identical electric charge.
Preferably, described emulsifying agent is one or more of citric acid monoglyceride, sucrose ester, lecithin, soybean lecithin, glycerine monofatty ester, and emulsifying agent addition is 0.1～0.3% of microcapsules gross mass.
Preferably, described packing step is 40～80 ℃ of temperature, add a certain proportion of liquid compound tree peony seed oil formulation, homogeneous 5～the 10min of 3000～6000r/min high speed forms the emulsion of homogeneous, with acid for adjusting pH value to 3.2～4.2 (isoelectric point of tree peony cake cake protein is 4.5), make tree peony cake cake protein and sodium alginate with opposite charges, and stirring at low speed 20～25min, the multiple condensed phase that wall material generation complex coacervation produces is wrapped in liquid compound tree peony seed oil formulation surface, form microcapsule suspensions, be diluted with water and be placed in ice bath and continue to be stirred to below 10 ℃, make the gelation of gained complex coacervation thing.
Preferably, described curing, drying steps, is to add curing agent, and stirring at low speed is solidified 1～2h, and stratification is collected the capsule that wets after filtering, washing, and through 30～50 ℃ of vacuum freeze dryings, obtains Powdered compound tree peony seed oil formulation.
Preferably, described curing agent is one or more of glucono-δ-lactone, glucose, pectin, tannic acid, and the addition of curing agent is 0.8～2% of microcapsules gross mass.
Preferred version (4), described wall material is one or more in cyclodextrin, maltodextrin, starch octenyl succinate anhydride, shitosan, lactose, converted starch, xanthans, guar gum, Arabic gum, lactalbumin, alcohol soluble protein, casein, soybean protein isolate.
Preferred, described wall material is converted starch, Arabic gum and maltodextrin, and further, the mass ratio of converted starch, Arabic gum and maltodextrin is 1: 0.8～1.2: 0.8～1.2, and core and wall material mass ratio are 2～4: 1.
Preferably, described method comprises that take liquid compound tree peony seed oil formulation is core, and above-mentioned material is wall material, through preparation, (2) emulsification, the dry preparation of (3) spraying of following steps (1) wall material and obtain.
Preferably, the preparation process of described wall material is converted starch, Arabic gum, maltodextrin to be dissolved in the hot water of 60～65 ℃, adds while stirring liquid compound tree peony seed oil formulation.
Preferred, in above-mentioned solution, total solids content is 30%.
Preferably, described emulsifying step is continue to stir 10～15min, by colloid mill homogeneous 2～3 times or homogenization pressure 20～50Mpa, 30～50 ℃ carry out the high-pressure homogeneous emulsion that makes.
Preferably, described spraying drying steps is 140～180 ℃ of EATs, and 80～100 ℃ of leaving air temps enter spray dryer by emulsion with flow 20ml/min dry, obtain Powdered compound tree peony seed oil formulation.
The present invention also provides a kind of compound tree peony seed oil formulation, by above-mentioned arbitrary method, is prepared from.
Preferably, described compound tree peony seed oil formulation can be liquid state, powder or further be processed into the forms such as particle electuary, tablet, capsule, oral liquid.
The present invention also provides a kind of application of above-mentioned compound tree peony seed oil formulation, described in be applied as health-care oil food, health-care oil or food additives etc.
Preferably, described health food have significant reducing blood lipid, hypotensive, reduce cholesterol, improve cardiovascular and cerebrovascular disease, the effect of antitumor, antifatigue.。
Compound tree peony seed oil formulation prepared by the present invention, is conducive to tocotrienols and red colouring agent for food, also used as a Chinese medicine absorbs effectively, is also beneficial to peony seed oil and preserves for a long time, the collaborative physiological function of performance three simultaneously.This compound tree peony seed oil formulation, can adopt particle electuary type or soft capsule dosage form or Tabules or oral liquid formulation, have safe and efficient, bioavilability is high, steady quality, can industrialization feature, and have significant reducing blood lipid, hypotensive, reduce cholesterol, improve cardiovascular and cerebrovascular disease, the effect of antitumor, antifatigue.
The specific embodiment
Below in conjunction with embodiment, further illustrate the present invention, should be appreciated that these examples can not be as restriction of the present invention, without departing from the spirit and substance of the case in the present invention, the modification of doing or replacement all belong to scope of the present invention.If do not specialize, the means in following embodiment are conventional means known in the art.
The preparation of the liquid compound tree peony seed of embodiment 1 oil formulation
The peony seed oil of 85 parts is heated to 60 ℃, adds the tocotrienols of 5 parts and the red colouring agent for food, also used as a Chinese medicine of 10 parts, stir, homogeneous preparation becomes the liquid compound tree peony seed oil formulation of stable homogeneous.
The preparation of the liquid compound tree peony seed of embodiment 2 oil formulation
The peony seed oil of 55 parts is heated to 70 ℃, adds the tocotrienols of 40 parts and the red colouring agent for food, also used as a Chinese medicine of 5 parts, stir, homogeneous preparation becomes the liquid compound tree peony seed oil formulation of stable homogeneous.
The preparation of the liquid compound tree peony seed of embodiment 3 oil formulation
The peony seed oil of 30 parts is heated to 80 ℃, adds the tocotrienols of 50 parts and the red colouring agent for food, also used as a Chinese medicine of 20 parts, stir, homogeneous preparation becomes the liquid compound tree peony seed oil formulation of stable homogeneous.
The preparation of the liquid compound tree peony seed of embodiment 4 oil formulation
The peony seed oil of 98.9 parts is heated to 60 ℃, adds the tocotrienols of 0.1 part and the red colouring agent for food, also used as a Chinese medicine of 1 part, stir, homogeneous preparation becomes the liquid compound tree peony seed oil formulation of stable homogeneous.
The preparation of the liquid compound tree peony seed of embodiment 5 oil formulation
The peony seed oil of 40 parts is heated to 80 ℃, adds the tocotrienols of 60 parts, stir, homogeneous preparation becomes the liquid compound tree peony seed oil formulation of stable homogeneous.
The preparation of the liquid compound tree peony seed of embodiment 6 oil formulation
The peony seed oil of 99.9 parts is heated to 70 ℃, adds the tocotrienols of 0.1 part, stir, homogeneous preparation becomes the liquid compound tree peony seed oil formulation of stable homogeneous.
Embodiment 7 yeast microcapsule methods are prepared Powdered compound tree peony seed oil formulation
Step 1 waste yeast liquid purifying
Get wine brewing or antibiotics plant waste yeast liquid, sterile water wash with 2 times of volumes, with 80 orders, 100 object sieves, sieve respectively 2 times, remove hop resin and other larger impurity, centrifugal 5min under 5000r/min normal temperature, abandon supernatant, use again sterile water wash 1～2 time, to supernatant colorless and odorless, centrifugally obtain milky yeast milk, dry through vacuum drying or spraying, make dry ferment particle.
Step 2 yeast autolysis is prepared wall material
Get the yeast after purifying, add the water of 4 times to make suspension, add again autolysis promoter 0.5% ethanol, and adjust Initial pH 6, changing temperature autolysis 48h within the scope of 40～60 ℃, at 80 ℃, enzyme 15min goes out, after 3000r/min centrifugation 40min, it is standby that 30 ℃ of vacuum drying or freeze drying obtain yeast cell wall.
The preparation of the Powdered compound tree peony seed of step 3 oil formulation
The arbitrary liquid compound tree peony seed oil formulation of the embodiment 1-4 of take is core, yeast after processing is wall material, in core: 1: 2 ratio of wall material mass ratio adds liquid compound tree peony seed oil formulation and yeast sealing, put into 50 ℃ of water bath with thermostatic control oscillators, frequency of oscillation 80r/min, vibration 12h, compound tree peony seed oil formulation can enter yeast cells inside by diffusion effect, form microcapsules, vacuum filtration reclaims the compound tree peony seed oil formulation not wrapping into, use again 2 yeast surface raffinates of absolute ethanol washing, 30 ℃ of vacuum drying 30min or freeze drying, obtain Powdered compound tree peony seed oil formulation.
Embodiment 8 yeast microcapsule methods are prepared Powdered compound tree peony seed oil formulation
The deactivation of step 1 dry ferment
By 65 ℃ of deactivation 30min of food grade dry ferment, or ultra violet lamp deactivation.
Step 2 yeast autolysis is prepared wall material
Get the yeast after purifying, add the water of 10 times to make suspension, add again autolysis promoter 5% ethanol, and adjust Initial pH 7, changing temperature autolysis 12h within the scope of 40～60 ℃, at 95 ℃, enzyme 10min goes out, after 5000r/min centrifugation 30min, it is standby that 50 ℃ of vacuum drying or freeze drying obtain yeast cell wall.
The preparation of the Powdered compound tree peony seed of step 3 oil formulation
The arbitrary liquid compound tree peony seed oil formulation of the embodiment 1-4 of take is core, yeast after processing is wall material, in core: 3: 1 ratios of wall material mass ratio add liquid compound tree peony seed oil formulation and yeast sealing, put into 50 ℃ of water bath with thermostatic control oscillators, frequency of oscillation 160r/min, vibration 6h, compound tree peony seed oil formulation can enter yeast cells inside by diffusion effect, form microcapsules, vacuum filtration reclaims the compound tree peony seed oil formulation not wrapping into, use again 2 yeast surface raffinates of absolute ethanol washing, 50 ℃ of vacuum drying 20min or freeze drying, obtain Powdered compound tree peony seed oil formulation.
Embodiment 9 yeast microcapsule methods are prepared Powdered compound tree peony seed oil formulation
Step 1 waste yeast liquid purifying
Get wine brewing or antibiotics plant waste yeast liquid, sterile water wash with 3 times of volumes, with 80 orders, 100 object sieves, sieve respectively 2 times, remove hop resin and other larger impurity, centrifugal 10min under 5000r/min normal temperature, abandon supernatant, use again sterile water wash 1～2 time, to supernatant colorless and odorless, centrifugally obtain milky yeast milk, dry through vacuum drying or spraying, make dry ferment particle.
Step 2 yeast autolysis is prepared wall material
Get the yeast after purifying, add the water of 6 times to make suspension, add again autolysis promoter 1% ethanol, and adjust Initial pH 6, changing temperature autolysis 36h within the scope of 40～60 ℃, at 90 ℃, enzyme 12min goes out, after 4000r/min centrifugation 35min, it is standby that 30 ℃ of vacuum drying or freeze drying obtain yeast cell wall.
The preparation of the Powdered compound tree peony seed of step 3 oil formulation
The arbitrary liquid compound tree peony seed oil formulation of the embodiment 5-6 of take is core, yeast after processing is wall material, in core: 1: 1 ratio of wall material mass ratio adds liquid compound tree peony seed oil formulation and yeast sealing, put into 50 ℃ of water bath with thermostatic control oscillators, frequency of oscillation 100r/min, vibration 10h, compound tree peony seed oil formulation can enter yeast cells inside by diffusion effect, form microcapsules, vacuum filtration reclaims the compound tree peony seed oil formulation not wrapping into, use again 2 yeast surface raffinates of absolute ethanol washing, 40 ℃ of vacuum drying 25min or freeze drying, obtain Powdered compound tree peony seed oil formulation.
Embodiment 10 yeast microcapsule methods are prepared Powdered compound tree peony seed oil formulation
The deactivation of step 1 dry ferment
By 65 ℃ of deactivation 30min of food grade dry ferment, or ultra violet lamp deactivation.
Step 2 yeast autolysis is prepared wall material
Get the yeast after purifying, add the water of 8 times to make suspension, add again autolysis promoter 3% ethanol, and adjust Initial pH 5, changing temperature autolysis 24h within the scope of 40～60 ℃, at 95 ℃, enzyme 10min goes out, after 5000r/min centrifugation 30min, it is standby that 50 ℃ of vacuum drying or freeze drying obtain yeast cell wall.
The preparation of the Powdered compound tree peony seed of step 3 oil formulation
The arbitrary liquid compound tree peony seed oil formulation of the embodiment 5-6 of take is core, yeast after processing is wall material, in core: 2: 1 ratios of wall material mass ratio add liquid compound tree peony seed oil formulation and yeast sealing, put into 50 ℃ of water bath with thermostatic control oscillators, frequency of oscillation 120r/min, vibration 8h, compound tree peony seed oil formulation can enter yeast cells inside by diffusion effect, form microcapsules, vacuum filtration reclaims the compound tree peony seed oil formulation not wrapping into, use again 2 yeast surface raffinates of absolute ethanol washing, 50 ℃ of vacuum drying 20min or freeze drying, obtain Powdered compound tree peony seed oil formulation.
Embodiment 11 soybean protein isolates and sodium alginate complex coacervation are prepared Powdered compound tree peony seed oil formulation
Step 1, with soybean protein isolate and sodium alginate, be wall material, mass ratio is 2: 1, adds respectively the water of 30 times, and soybean protein isolate and sodium alginate are configured to about 3.33% solution.
Step 2, in soybean protein isolate solution, add emulsifying agent citric acid monoglyceride, addition is 0.1% of microcapsules gross mass, and stirring and dissolving at 60 ℃, by the pH value to 9 of alkali regulator solution.
Step 3, in above-mentioned solution, to add the arbitrary liquid compound tree peony seed oil formulation of embodiment 1-4 be core, core and wall material mass ratio are 1: 2, at 40 ℃ of temperature, with the speed high-speed stirred 5min of 6000r/min, obtain the emulsion of stable homogeneous, and with acid for adjusting pH value to 4, to above-mentioned emulsion droplet, add sodium alginate soln and stir again, water bath heat preservation reaction 25min, forms complex coacervation thing in system.The water dilution cooling of the amount of doubling, and be placed in ice bath and continue to be stirred to below 10 ℃, make the gelation of gained complex coacervation thing.
Step 4, to above-mentioned system, add the glucono-δ-lactone of microcapsules gross mass 0.8%, at 40 ℃ of water-baths, solidify 2h, filtration washing precipitation, 30 ℃ of vacuum freeze dryings obtain Powdered compound tree peony seed oil formulation.
Embodiment 12 soybean protein isolates and sodium alginate complex coacervation are prepared Powdered compound tree peony seed oil formulation
Step 1, with soybean protein isolate and sodium alginate, be wall material, mass ratio is 5: 1, adds respectively the water of 20 times, and soybean protein isolate and sodium alginate are configured to about 5% solution.
Step 2, in soybean protein isolate solution, add emulsifier lecithin and soybean lecithin, addition is 0.3% of microcapsules gross mass, lecithin: soybean lecithin mass ratio is 1: 1, and stirring and dissolving at 80 ℃, by the pH value to 7 of alkali regulator solution.
Step 3, in above-mentioned solution, to add the arbitrary liquid compound tree peony seed oil formulation of embodiment 5-6 be core, core and wall material mass ratio are 2: 1, at 80 ℃ of temperature, with the speed high-speed stirred 10min of 3000r/min, obtain the emulsion of stable homogeneous, and with acid for adjusting pH value to 3, to above-mentioned emulsion droplet, add sodium alginate soln and stir again, water bath heat preservation reaction 20min, forms complex coacervation thing in system.The water dilution cooling of the amount of doubling, and be placed in ice bath and continue to be stirred to below 10 ℃, make the gelation of gained complex coacervation thing.
Step 4, to above-mentioned system, add the glucose of microcapsules gross mass 2%, at 80 ℃ of water-baths, solidify 1h, filtration washing precipitation, 50 ℃ of vacuum freeze dryings obtain Powdered compound tree peony seed oil formulation.
Embodiment 13 tree peony cake cake proteins and sodium alginate complex coacervation are prepared Powdered compound tree peony seed oil formulation
Step 1, with tree peony cake cake protein and sodium alginate, be wall material, mass ratio is 3: 1, adds respectively the water of 25 times, and tree peony cake cake protein and sodium alginate are configured to about 4% solution.
Step 2, in tree peony cake cake protein solution, add emulsifying agent sucrose ester, addition is 0.2% of microcapsules gross mass, and stirring and dissolving at 70 ℃, by the pH value to 8 of alkali regulator solution.
Step 3, in above-mentioned solution, to add the arbitrary liquid compound tree peony seed oil formulation of embodiment 1-4 be core, core and wall material mass ratio are 1: 1, at 60 ℃ of temperature, with the speed high-speed stirred 8min of 4500r/min, obtain the emulsion of stable homogeneous, and with acid for adjusting pH value to 3.2, to above-mentioned emulsion droplet, add sodium alginate soln and stir again, water bath heat preservation reaction 20min, forms complex coacervation thing in system.The water dilution cooling of the amount of doubling, and be placed in ice bath and continue to be stirred to below 10 ℃, make the gelation of gained complex coacervation thing.
Step 4, to above-mentioned system, add the pectin of microcapsules gross mass 1.5%, at 60 ℃ of water-baths, solidify 1.5h, filtration washing precipitation, 30 ℃ of vacuum freeze dryings obtain Powdered compound tree peony seed oil formulation.
Embodiment 14 tree peony cake cake proteins and sodium alginate complex coacervation are prepared Powdered compound tree peony seed oil formulation
Step 1, with tree peony cake cake protein and sodium alginate, be wall material, mass ratio is 4: 1, adds respectively the water of 20 times, and tree peony cake cake protein and sodium alginate are configured to about 5% solution.
Step 2, in tree peony cake cake protein solution, add emulsifying agent citric acid monoglyceride and glycerine monofatty ester, addition is 0.15% of microcapsules gross mass, citric acid monoglyceride and glycerine monofatty ester mass ratio are 2: 1, and stirring and dissolving at 60 ℃, by the pH value to 7 of alkali regulator solution.
Step 3, in above-mentioned solution, to add the arbitrary liquid compound tree peony seed oil formulation of embodiment 5-6 be core, core and wall material mass ratio are 1: 2, at 40 ℃ of temperature, with the speed high-speed stirred 10min of 6000r/min, obtain the emulsion of stable homogeneous, and with acid for adjusting pH value to 4.2, to above-mentioned emulsion droplet, add sodium alginate soln and stir again, water bath heat preservation reaction 20min, forms complex coacervation thing in system.The water dilution cooling of the amount of doubling, and be placed in ice bath and continue to be stirred to below 10 ℃, make the gelation of gained complex coacervation thing.
Step 4, to above-mentioned system, add glucose and the tannic acid of microcapsules gross mass 1.5%, glucose: tannic acid mass ratio is 1: 1, solidifies 1h at 70 ℃ of water-baths, filtration washing precipitation, and 30 ℃ of vacuum freeze dryings obtain Powdered compound tree peony seed oil formulation.
The dry microcapsule method of embodiment 15 spraying is prepared Powdered compound tree peony seed oil formulation
Step 1, converted starch, Arabic gum, maltodextrin are dissolved in by the mass ratio of 1: 1: 1 in the hot water of 60 ℃, add while stirring the arbitrary liquid compound tree peony seed oil formulation of embodiment 1-4.Wherein, in above-mentioned solution, total solids content is 30%.
Step 2, continue to stir 15min, by colloid mill homogeneous 2～3 times or homogenization pressure 40Mpa, 40 ℃ carry out the high-pressure homogeneous emulsion that makes.
Step 3, at 160 ℃ of EATs, 90 ℃ of leaving air temps, emulsion is entered to spray dryer with flow 20ml/min dry, obtain Powdered compound tree peony seed oil formulation.
The dry microcapsule method of embodiment 16 spraying is prepared Powdered compound tree peony seed oil formulation
Step 1, converted starch, Arabic gum, maltodextrin are dissolved in by the mass ratio of 1: 0.8: 1.2 in the hot water of 65 ℃, add while stirring the arbitrary liquid compound tree peony seed oil formulation of embodiment 5-6.Wherein, in above-mentioned solution, total solids content is 30%.
Step 2, continue to stir 10min, by colloid mill homogeneous 2～3 times or homogenization pressure 50Mpa, 30 ℃ carry out the high-pressure homogeneous emulsion that makes.
Step 3, at 140 ℃ of EATs, 100 ℃ of leaving air temps, emulsion is entered to spray dryer with flow 20ml/min dry, obtain Powdered compound tree peony seed oil formulation.
The preparation of embodiment 17 compound tree peony seed oil soft capsules
Step 1, according to following percentage by weight, take each component: gelatin 36.0%, glycerine 18.0%, methyl p-hydroxybenzoate 0.06%, water 45.94%.By formula ratio, take after above-mentioned raw materials, in inputization glue tank, change glue; The solution of gained is transferred to after by colloid mill in the bucket with attemperator, is connected on soft capsule pellet press, for the preparation of soft capsule peel.
Step 2, according to following percentage by weight, take each component: compound tree peony seed oil formulation 98%, ditert-butylhydro quinone 0.2% that embodiment 1-6 is arbitrary.By formula ratio, take embodiment 1-6 arbitrary compound tree peony seed oil formulation and ditert-butylhydro quinone, be heated dissolving, stir.Above-mentioned solution is put into reservoir, by soft capsule pellet press, be injected in soft capsule peel, suppress; The soft capsule pressing is cleaned, and be cured by drying equipment; Last packing, makes compound tree peony seed oil soft capsule of the present invention.
The preparation of embodiment 18 compound tree peony seed oil oral liquids
According to following percentage by weight, take each component: liquid compound tree peony seed oil formulation 89.6%, honey 10.0%, stevioside 0.2%, acesulfame potassium 0.2% that embodiment 1-6 is arbitrary.By formula ratio, take above-mentioned each material, fully dissolve; Then gained solution is transferred in oral liquid filling machine, injects in container; Sterilizing, makes compound tree peony seed oil oral liquid of the present invention.
The preparation of embodiment 19 compound tree peony seed oil electuaries
According to following percentage by weight, take each component: Powdered compound tree peony seed oil formulation 35.0%, cane sugar powder 45.0%, converted starch 16.0%, citric acid 2.0%, malic acid 2.0% that embodiment 7-16 is arbitrary.
First above-mentioned each material is pulverized or sieved; By formula ratio take successively pulverize or sieve after material; Load weighted material is dropped into mixer, mix; Add again the edible ethanol (volume fraction is 70%) of above-mentioned raw materials gross mass 10～20% as wetting agent,, by granulator, granulate; The particle making is dried with drying equipment, by pelletizing machine, carries out whole grain subsequently; Last packing, makes compound tree peony seed oil particles electuary of the present invention.
The preparation of embodiment 20 compound tree peony seed oil tablets
According to following percentage by weight, take each component: Powdered compound tree peony seed oil formulation 35.0%, sucrose 28.5%, starch 10.0%, coated vitamin C 1.5%, microcrystalline cellulose 15.0%, dextrin 8.0%, film coating agent 1.0%, dolomol 0.6%, silica 0.4% that embodiment 7-16 is arbitrary.
First above-mentioned each material is pulverized or sieved; By formula ratio take successively pulverize or sieve after material; Load weighted Powdered compound tree peony seed oil formulation, starch, coated vitamin C, sucrose, microcrystalline cellulose and dextrin are dropped into mixer, mix; Add again the edible ethanol (volume fraction is 70%) of above-mentioned raw materials gross mass 10～20% as wetting agent, by granulator, to granulate; The particle making is dried with drying equipment, by pelletizing machine, carries out whole grain subsequently; Add dolomol, silica always to mix, the material being always mixed after even is carried out to compressing tablet by tablet press machine, plain sheet is temporary, standby; Film coating agent is added in suitable quantity of water, stir, be mixed with concentration and be 5.0～15.0% coating solution, by seed-coating machine, the plain sheet pressing is carried out to Cotton seeds; Last packing, makes compound tree peony seed oil formulation tablet of the present invention.
The preparation of embodiment 21 compound tree peony seed oil capsules
According to following percentage by weight, take each component: Powdered compound tree peony seed oil formulation 54.0%, coated vitamin C 9.0%, microcrystalline cellulose 36.0%, dolomol 0.6%, silica 0.4% that embodiment 7-16 is arbitrary.
First above-mentioned each material is pulverized, sieved; By formula ratio take successively pulverizing, material after sieving; Load weighted material is dropped into mixer, mix; Then by capsule filler, capsule is filled; Last packing, makes compound tree peony seed oil formulation capsule of the present invention.
The preparation of embodiment 22 compound tree peony seed oil oral liquids
According to following percentage by weight, take each component: Powdered compound tree peony seed oil formulation 30.0%, honey 10.0%, fructose 20.0%, vitamin C sodium salt 5%, malic acid 2.0%, citric acid 1.6%, carragheen 1.0%, stevioside 0.2%, acesulfame potassium 0.2%, 30% pure water that embodiment 7-16 is arbitrary.
By formula ratio, take above-mentioned each material, drop in water, fully dissolve; Then gained solution is transferred in oral liquid filling machine, injects in container; Sterilizing, makes compound tree peony seed oil formulation oral liquid of the present invention.
One, the compound tree peony seed oil formulation product making in order to evaluate the present invention, at room temperature, we,, from acid value and two data of peroxide value of product, evaluate the stability of compound tree peony seed oil formulation.Each sample is chosen 10 samples and is measured, and following list data is mean value.
Index explanation:
1, the KOH milligram number that contained free fatty needs during acid value refers to and in 1g grease, the mg/g of unit.Useful acid value is carried out the freshness of measure oil.National standard " standard " the fresh food vegetable oil sanitary standard of the measure oil " acid value≤3mg/g of family.
2, peroxide value refers to the amount of peroxides percentage composition producing in Oxidation of Fat and Oils process, the g/100g of unit.Assay method is separated out I for ROOH and KI effect under acid condition
2, then use Na
2s
2o
3titration, mM number calculating, is mM number of ROOH.Its size has reflected degree spoiled by rancid oil or fat, and in general higher its of peroxide value becomes sour just more severe.National standard " family's standard " in general degree crosses edible vegetable oil sanitary standard " peroxide value≤0.25g/100g.
3, the Powdered compound tree peony seed oil formulation of embodiment 7,8,11,13,15 be take the liquid compound tree peony seed oil of embodiment 4 and is illustrated as raw material, and the Powdered compound tree peony seed oil formulation of embodiment 9,10,12,14,16 be take the liquid compound tree peony seed oil of embodiment 6 and illustrated as raw material.
4, because the Powdered compound tree peony seed oil formulation of embodiment 7-16 contains wall material, can disturb the mensuration of acid value and peroxide value, need to carry out pretreatment to sample.Processing method: take benzinum as solvent, the liquid compound tree peony seed oil of sonic oscillation extraction.In 50mL hard glass test tube, accurately add the about 5g of Powdered compound tree peony seed oil formulation sample of embodiment 7-16, pour 25mL benzinum into, be placed in ultrasonic cleaner tank sonic oscillation 30min; Take out, filter, and with benzinum, fully wash merging filtrate, Rotary Evaporators reclaims benzinum, heating water bath, and the benzinum that fully volatilizees is placed in vacuum drying chamber, further remove benzinum for 50 ℃, obtain liquid compound tree peony seed oil, and then measure acid value and peroxide value.
Result shows, embodiment 6 is the compound preparation that tocotrienols is minimum, embodiment 5 is the highest compound preparation of tocotrienols content, the acid value that came into effect example 5 and 6 from three month demonstrates the difference of conspicuousness (p < 0.05), compare with single peony seed oil, acid value and peroxide value all demonstrate the difference (p < 0.05) of conspicuousness simultaneously.Since 6th month, embodiment 5 and 6 peroxide value demonstrated the difference (p < 0.05) of conspicuousness.The above results explanation, with respect to single peony seed oil, adds the freshness that can extend peony seed oil after tocotrienols, reduces the degree of spoilage of grease, and along with the concentration of tocotrienols increases, its above-mentioned effect also increases, and becomes positive correlation.
Result shows, embodiment 4 is tocotrienols and the minimum compound preparation of red colouring agent for food, also used as a Chinese medicine content, embodiment 3 is tocotrienols and the highest compound preparation of red colouring agent for food, also used as a Chinese medicine content, the acid value that came into effect example 3 and 4 from three month demonstrates the difference of conspicuousness (p < 0.05), compare with single peony seed oil, acid value and peroxide value all demonstrate the difference (p < 0.05) of conspicuousness simultaneously.Since 6th month, embodiment 3 and 4 peroxide value demonstrated the difference (p < 0.05) of conspicuousness.The above results explanation, with respect to single peony seed oil, adds the freshness that can extend peony seed oil after tocotrienols and red colouring agent for food, also used as a Chinese medicine, reduces the degree of spoilage of grease, and along with the concentration of tocotrienols and red colouring agent for food, also used as a Chinese medicine increases, its above-mentioned effect also increases, and becomes positive correlation.
The Powdered compound tree peony seed oil formulation of embodiment 7,8,11,13,15 be take the liquid compound tree peony seed oil of tocotrienols and the minimum embodiment 4 of red colouring agent for food, also used as a Chinese medicine content and is illustrated as raw material, the Powdered compound tree peony seed oil formulation of embodiment 9,10,12,14,16 be take the liquid compound tree peony seed oil of the minimum embodiment 6 of tocotrienols content and is illustrated as raw material, above-described embodiment was since three month, compare with single peony seed oil, acid value and peroxide value all demonstrate the difference (p < 0.05) of conspicuousness.The above results explanation, the freshness that Powdered compound tree peony seed oil formulation can extend peony seed oil, the degree of spoilage of reduction grease, and, those skilled in the art can know by inference when tocotrienols content or tocotrienols and the increase of red colouring agent for food, also used as a Chinese medicine content, and its effect can be better.
Result confirms simultaneously, the Powdered compound tree peony seed oil formulation of embodiment 7,8,11,13,15 also demonstrated the difference (p < 0.05) of conspicuousness since three month acid value with respect to the liquid compound tree peony seed oil of embodiment 4, peroxide value demonstrated the difference (p < 0.05) of conspicuousness since 9th month.Identical result also appears in the contrast of the Powdered compound tree peony seed oil formulation of embodiment 9,10,12,14,16 and the liquid compound tree peony seed oil of embodiment 6, the above results explanation, micro-embedded technology can further extend the freshness of peony seed oil, reduces the degree of spoilage of grease.
Two, we have also measured the bioavilability of tocotrienols in each embodiment or red colouring agent for food, also used as a Chinese medicine.Each sample is chosen 10 samples and is measured, and following list data is mean value.
| ? | Tocotrienols bioavilability | Red colouring agent for food, also used as a Chinese medicine bioavilability |
| Tocotrienols | 100％ | ? |
| Red colouring agent for food, also used as a Chinese medicine | ? | 100％ |
| Embodiment 1 | 175％ | 142％ |
| Embodiment 2 | 153％ | 158％ |
| Embodiment 3 | 127％ | 126％ |
| Embodiment 4 | 204％ | 191％ |
| Embodiment 5 | 118％ | ? |
| Embodiment 6 | 199％ | ? |
Index explanation:
1, with the dosage gastric infusion of purifying tocotrienols 50mg/kg to mouse, measure the concentration of tocotrienols in vein, in contrast, the bioavailability of setting this numerical value is 100%, other embodiment are with preparation corresponding to Isodose tocotrienols (50mg/kg), gastric infusion, to mouse, is measured the relative bioavailability of this embodiment under the same conditions.Embodiment 1,2,3,4,5,6 is followed successively by 1g/kg, 125mg/kg, 100mg/kg, 50g/kg, 83.3mg/kg, 50g/kg to the amount of formulation of mouse.
2, with the dosage gastric infusion of red colouring agent for food, also used as a Chinese medicine 50mg/kg to mouse, measure the concentration of red colouring agent for food, also used as a Chinese medicine in vein, in contrast, the bioavailability of setting this numerical value is 100%, other embodiment are with preparation corresponding to Isodose red colouring agent for food, also used as a Chinese medicine (50mg/kg), gastric infusion, to mouse, is measured the relative bioavailability of this embodiment under the same conditions.Embodiment 1,2,3,4 is followed successively by 500mg/kg, 1g/kg, 250mg/kg, 5g/kg to the amount of formulation of mouse.
Result shows, embodiment 4 and 6 is the compound preparation that tocotrienols concentration is minimum, embodiment 5 is the highest compound preparation of tocotrienols concentration, compare with embodiment 5, embodiment 4 and 6 tocotrienols bioavailability demonstrate the difference (p < 0.05) of conspicuousness, compare with single tocotrienols, bioavailability also demonstrates the difference (p < 0.05) of conspicuousness simultaneously.The above results explanation, with respect to single tocotrienols, can improve the bioavailability of tocotrienols after interpolation peony seed oil, and along with the concentration of tocotrienols reduces, its above-mentioned effect also increases, and becomes negative correlation.
Result shows, embodiment 4 is the compound preparation that red colouring agent for food, also used as a Chinese medicine concentration is minimum, embodiment 3 is the highest compound preparation of red colouring agent for food, also used as a Chinese medicine concentration, compare with embodiment 4, the red colouring agent for food, also used as a Chinese medicine bioavailability of embodiment 3 demonstrates the difference (p < 0.05) of conspicuousness, compare with single red colouring agent for food, also used as a Chinese medicine, bioavailability also demonstrates the difference (p < 0.05) of conspicuousness simultaneously.The above results explanation, with respect to single red colouring agent for food, also used as a Chinese medicine, can improve the bioavailability of red colouring agent for food, also used as a Chinese medicine after interpolation peony seed oil, and along with the concentration of red colouring agent for food, also used as a Chinese medicine reduces, its above-mentioned effect also increases, and becomes negative correlation.
Claims (9)
1. a compound tree peony seed oil formulation, is characterized in that, described compound tree peony seed oil formulation comprises peony seed oil and tocotrienols.
2. compound tree peony seed oil formulation as claimed in claim 1, is characterized in that, described preparation also comprises red colouring agent for food, also used as a Chinese medicine.
3. compound tree peony seed oil formulation as claimed in claim 1, is characterized in that, described preparation includes following component by weight: tocotrienols 0.1%～60%, peony seed oil 40%～99.9%.
4. compound tree peony seed oil formulation as claimed in claim 2, is characterized in that, described preparation includes following component by weight: tocotrienols 0.1%～50%, red colouring agent for food, also used as a Chinese medicine 1%～20%, peony seed oil 30%～98.9%.
5. compound tree peony seed oil formulation as arbitrary in claim 1-4, it is characterized in that, described preparation is liquid compound tree peony seed oil formulation, or pass through microcapsules technology, by being dissolved with the peony seed oil parcel of tocotrienols or tocotrienols and red colouring agent for food, also used as a Chinese medicine, be prepared into Powdered compound tree peony seed oil formulation or be further processed into the forms such as particle electuary, tablet, capsule, oral liquid.
6. as the preparation method of the arbitrary preparation of claim 1-4, described method comprises peony seed oil is heated to 50～80 ℃, the tocotrienols or tocotrienols and the red colouring agent for food, also used as a Chinese medicine that add corresponding proportion, stir, and homogeneous preparation becomes the liquid compound tree peony seed oil formulation of stable homogeneous.
7. preparation method as claimed in claim 6, is characterized in that, further comprises that liquid compound tree peony seed oil formulation is core, and one of following component is wall material, by microcapsules technology, prepares Powdered compound tree peony seed oil formulation:
(1) yeast;
(2) soybean protein isolate and sodium alginate;
(3) tree peony cake cake protein and sodium alginate; Or
(4) one or more in cyclodextrin, maltodextrin, starch octenyl succinate anhydride, shitosan, lactose, xanthans, guar gum, Arabic gum.
8. the preparation method as described in claim 6 or 7, is characterized in that, described preparation can further be processed into the forms such as particle electuary, tablet, capsule, oral liquid.
9. the application of compound tree peony seed oil formulation as arbitrary in claim 1-4, is characterized in that, described in be applied as health food, health-care oil or food additives etc.
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| CN109619586A (en) * | 2019-01-24 | 2019-04-16 | 山西潞安石圪节智华生物科技有限公司 | A kind of peony seed oil blood fat reducing preparation and preparation method thereof |
| CN109699887A (en) * | 2019-01-24 | 2019-05-03 | 山西潞安石圪节智华生物科技有限公司 | A kind of peony seed oil blood fat reducing soft capsule and preparation method thereof |
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| CN116098937A (en) * | 2022-12-02 | 2023-05-12 | 山东省科学院菏泽分院 | Application of peony seed oil in cerebral ischemia reperfusion injury protection |
| CN116076724A (en) * | 2023-02-06 | 2023-05-09 | 菏泽牡丹产业技术研究院 | Peony seed oil oral liquid with blood sugar reducing effect and preparation method thereof |
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