CN104000051A - Pig feed complex enzyme preparation and preparing method thereof - Google Patents
Pig feed complex enzyme preparation and preparing method thereof Download PDFInfo
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- CN104000051A CN104000051A CN201410275557.6A CN201410275557A CN104000051A CN 104000051 A CN104000051 A CN 104000051A CN 201410275557 A CN201410275557 A CN 201410275557A CN 104000051 A CN104000051 A CN 104000051A
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Abstract
The invention provides a pig feed complex enzyme preparation and belongs to the field of feed additives. The additive is composed of a complex enzyme and a fortifier, wherein the complex enzyme is the combination of lipase, neutral protease, beta-amylase, isoamylase, neutral phytase, beta-mannase, xylanase and alpha-galactosidase, and the fortifier is composed of pine needle extracts, bamboo leaf crude polysaccharide, honeysuckle stem powder, galangal powder, common vladimiria root powder, lithospermum powder, vitamin C, sodium chloride, sodium butyrate, ferrous sulfate and calcium gluconate. By the adoption of the enzyme preparation, the utilization rate of feed can be effectively increased, the feed conversion ratio can be reduced, the growing speed of pigs can be increased, the immunity of pigs can be improved, the health degree of pigs can be increased, the morbidity of pigs can be reduced, and market prospects are quite broad.
Description
Technical field
The present invention relates to a kind of enzyme preparation, be specifically related to a kind of pig's feed complex enzyme formulation, belong to feed additive field.
Background technology
Enzyme preparation as feed addictive to improving animal feed utilization rate, eliminate the ANFs in feed, the abilities of digestive and absorption of raising animal etc. has very positive effect, developed country since the 70's of 20th century just in feed, add the application study of enzyme preparation, and China's feed industry is just progressively carried out the application of enzyme preparation in the 90's of 20th century.At present, the feed that countries in Europe adds enzyme preparation has accounted for more than 90%, and in contrast to this, China also has very large gap.Therefore, strengthen the research to enzyme Preparations Used for Feeds, to promoting China's aquaculture development to there is very positive meaning.Fodder enzyme preparation can promote to digest and assimilate, and reduces the pollution to environment; Reduce chymeviscosity, improve the digestibility of feed; Improve the ratio of digestible nutrient, improve food utilization efficiency; Eliminate ANFs in feed; Improve gut flora state, improve animal health level.Internal feed additive technology mostly adopts with the raw material after pulverizing and directly mixes as feed; or only add exogenous enzymes when raw material processing; do not add some protective agent or zymoexciter; and feed link is everlasting in open environment and is used; make exogenous enzymes at storage and activity decreased often when feeding, affect the Forage Nutritive Value of feed itself.
Summary of the invention
The object of the present invention is to provide a kind of pig's feed complex enzyme formulation, said preparation can effectively improve the utilization rate of feed, reduces feedstuff-meat ratio, increases economic benefit; In addition in this complex enzyme formulation, be also added with reinforcing agent, can improve the reaction rate of enzyme, complex enzyme formulation is taken effect faster, and each composition in reinforcing agent and enzyme preparation is combined use and also can be strengthened the immunity of pig.
The present invention is achieved through the following technical solutions:
A kind of pig's feed complex enzyme formulation, is made up of complex enzyme and reinforcing agent, and the mass ratio of complex enzyme and reinforcing agent is 100:3-6;
Described complex enzyme is: in every gram of complex enzyme, the activity of various enzymes is as follows: lipase 600-1000 IU, neutral proteinase 1200-1600IU, beta amylase 450-650IU, isoamylase 100-200IU, neutral phytase 1600-2000IU, 'beta '-mannase 100-140IU, zytase 200-300IU, alpha-galactosidase 100-200IU;
Described reinforcing agent is made up of following raw materials by weight portion: extract from pine needles 1-3 part, the thick polysaccharide 2-4 of leaf of bamboo part, caulis lonicerae powder 2-4 part, galangal powder 2-4 part, radix jurineae powder 1-3 part, Asian puccoon powder 3-6 part, vitamin C 0.01-0.04 part, sodium chloride 0.04-0.06 part, sodium butyrate 0.01-0.03 part, ferrous sulfate 0.01-0.03 part, calcium gluconae 0.01-0.03 part;
The preparation method of described extract from pine needles is: by fresh pine needle impurity elimination, be placed in the dry 24h of vacuum drying chamber, baking temperature is 65 DEG C, and crushed after being dried is crossed 20 mesh sieves, obtains pine needle meal; By pine needle meal, cellulase and pectase in mass ratio 82:1-1.8:1-1.6 mix, in the mixture of pine needle meal, cellulase and pectase, add distilled water by solid-to-liquid ratio 1:10-15, at 32 DEG C, soak 24-48h; Immersion finishes rear filtration, and filtrate is adopted to centrifugal treating, and centrifugal rotational speed is 3600r/min, and centrifugation time is 5min, after centrifugal end, gets supernatant, by supernatant freeze drying, pulverizes 60 mesh sieves, obtains extract from pine needles;
The preparation method of the thick polysaccharide of the described leaf of bamboo: by leaf of bamboo drying and crushing, cross 30 mesh sieves, obtain bamboo leaf powder, bamboo leaf powder is mixed by 1:12-16 with distilled water, adopt refluxing extraction 40-60min, extraction temperature is 60-80 DEG C, extracts and finishes rear filtration, by filtrate concentrate drying, pulverized 60 mesh sieves and obtained the thick polysaccharide of the leaf of bamboo;
The preparation method of described caulis lonicerae powder is: dry caulis lonicerae was pulverized to 60 mesh sieves;
The preparation method of described galangal powder is: dry galangal was pulverized to 60 mesh sieves;
The preparation method of described radix jurineae powder is: dry radix jurineae was pulverized to 60 mesh sieves;
The preparation method of described Asian puccoon powder is: dry Asian puccoon was pulverized to 60 mesh sieves;
The preparation technology of described reinforcing agent is: (1) mixes caulis lonicerae powder, galangal powder, radix jurineae powder, Asian puccoon powder, in caulis lonicerae powder, galangal powder, radix jurineae powder, Asian puccoon powder mixture, add distilled water by solid-to-liquid ratio 1:5, at 40-50 DEG C, soak 1-3h, filter, filter residue and drying powder is crossed to 60 mesh sieves, stand-by; (2) sodium chloride, sodium butyrate, ferrous sulfate, calcium gluconae are mixed, stand-by; (3) gains in extract from pine needles, the thick polysaccharide of the leaf of bamboo, vitamin C and step (1), step (2) are mixed, obtain reinforcing agent.
In the present invention, a kind of preparation method of pig's feed complex enzyme formulation is as follows: complex enzyme is mixed and be get final product with reinforcing agent.
The addition of product of the present invention in pig feed is 600-1000 gram/ton.
Beneficial effect
The pig's feed complex enzyme formulation that the present invention makes, can effectively improve the utilization rate of feed, reduces feedstuff-meat ratio, promotes the speed of growth of pig, has very large help to increasing economic benefit; And in this complex enzyme formulation, be also added with reinforcing agent, can improve the reaction rate of enzyme, complex enzyme formulation is taken effect faster.In addition, inventor is in the favorite outer discovery of research process, and the complex enzyme formulation in the present invention can also play the effect of the immunity that strengthens pig, to improving the health degree of pig, reduces the pig incidence of disease and has very large help.
Specific embodiment
Further set forth the present invention by specific embodiment below.
Embodiment 1
A kind of pig's feed complex enzyme formulation, is made up of complex enzyme and reinforcing agent, and the mass ratio of complex enzyme and reinforcing agent is 100:3;
Described complex enzyme is: in every gram of complex enzyme, the activity of various enzymes is as follows: lipase 600IU, neutral proteinase 1200IU, beta amylase 450IU, isoamylase 100IU, neutral phytase 1600IU, 'beta '-mannase 100IU, zytase 200IU, alpha-galactosidase 100IU;
Described reinforcing agent is made up of following raw materials by weight portion: 1 part of extract from pine needles, 2 parts of the thick polysaccharide of the leaf of bamboo, 2 parts, caulis lonicerae powder, 2 parts, galangal powder, 1 part, radix jurineae powder, 3 parts, Asian puccoon powder, 0.01 part of vitamin C, 0.04 part, sodium chloride, 0.01 part of sodium butyrate, 0.01 part, ferrous sulfate, 0.01 part of calcium gluconae;
The preparation method of described extract from pine needles is: by fresh pine needle impurity elimination, be placed in the dry 24h of vacuum drying chamber, baking temperature is 65 DEG C, and crushed after being dried is crossed 20 mesh sieves, obtains pine needle meal; By pine needle meal, cellulase and pectase in mass ratio 82:1:1 mix, in the mixture of pine needle meal, cellulase and pectase, add distilled water by solid-to-liquid ratio 1:10, at 32 DEG C, soak 24h; Immersion finishes rear filtration, and filtrate is adopted to centrifugal treating, and centrifugal rotational speed is 3600r/min, and centrifugation time is 5min, after centrifugal end, gets supernatant, by supernatant freeze drying, pulverizes 60 mesh sieves, obtains extract from pine needles;
The preparation method of the thick polysaccharide of the described leaf of bamboo: by leaf of bamboo drying and crushing, cross 30 mesh sieves, obtain bamboo leaf powder, bamboo leaf powder is mixed by 1:12 with distilled water, adopt refluxing extraction 40min, extracting temperature is 60 DEG C, extraction finishes rear filtration, by filtrate concentrate drying, pulverizes 60 mesh sieves and obtains the thick polysaccharide of the leaf of bamboo;
The preparation method of described caulis lonicerae powder is: dry caulis lonicerae was pulverized to 60 mesh sieves;
The preparation method of described galangal powder is: dry galangal was pulverized to 60 mesh sieves;
The preparation method of described radix jurineae powder is: dry radix jurineae was pulverized to 60 mesh sieves;
The preparation method of described Asian puccoon powder is: dry Asian puccoon was pulverized to 60 mesh sieves;
The preparation technology of described reinforcing agent is: (1) mixes caulis lonicerae powder, galangal powder, radix jurineae powder, Asian puccoon powder, in caulis lonicerae powder, galangal powder, radix jurineae powder, Asian puccoon powder mixture, add distilled water by solid-to-liquid ratio 1:5, at 40-50 DEG C, soak 1h, filter, filter residue and drying powder is crossed to 60 mesh sieves, stand-by; (2) sodium chloride, sodium butyrate, ferrous sulfate, calcium gluconae are mixed, stand-by; (3) gains in extract from pine needles, the thick polysaccharide of the leaf of bamboo, vitamin C and step (1), step (2) are mixed, obtain reinforcing agent.
In the present invention, a kind of preparation method of pig's feed complex enzyme formulation is as follows: complex enzyme is mixed and be get final product with reinforcing agent.
The addition of product of the present invention in pig feed is 600 grams/ton.
Embodiment 2
A kind of pig's feed complex enzyme formulation, is made up of complex enzyme and reinforcing agent, and the mass ratio of complex enzyme and reinforcing agent is 100:5;
Described complex enzyme is: in every gram of complex enzyme, the activity of various enzymes is as follows: lipase 800 IU, neutral proteinase 1400IU, beta amylase 550 IU, isoamylase 150IU, neutral phytase 1800IU, 'beta '-mannase 120IU, zytase 250IU, alpha-galactosidase 150IU;
Described reinforcing agent is made up of following raw materials by weight portion: 2 parts of extract from pine needles, 3 parts of the thick polysaccharide of the leaf of bamboo, 3 parts, caulis lonicerae powder, 3 parts, galangal powder, 2 parts, radix jurineae powder, 5 parts, Asian puccoon powder, 0.03 part of vitamin C, 0.05 part, sodium chloride, 0.02 part of sodium butyrate, 0.02 part, ferrous sulfate, 0.02 part of calcium gluconae;
The preparation method of described extract from pine needles is: by fresh pine needle impurity elimination, be placed in the dry 24h of vacuum drying chamber, baking temperature is 65 DEG C, and crushed after being dried is crossed 20 mesh sieves, obtains pine needle meal; By pine needle meal, cellulase and pectase in mass ratio 82:1.4:1.3 mix, in the mixture of pine needle meal, cellulase and pectase, add distilled water by solid-to-liquid ratio 1:13, at 32 DEG C, soak 40h; Immersion finishes rear filtration, and filtrate is adopted to centrifugal treating, and centrifugal rotational speed is 3600r/min, and centrifugation time is 5min, after centrifugal end, gets supernatant, by supernatant freeze drying, pulverizes 60 mesh sieves, obtains extract from pine needles;
The preparation method of the thick polysaccharide of the described leaf of bamboo: by leaf of bamboo drying and crushing, cross 30 mesh sieves, obtain bamboo leaf powder, bamboo leaf powder is mixed by 1:14 with distilled water, adopt refluxing extraction 50min, extracting temperature is 70 DEG C, extraction finishes rear filtration, by filtrate concentrate drying, pulverizes 60 mesh sieves and obtains the thick polysaccharide of the leaf of bamboo;
The preparation method of described caulis lonicerae powder is: dry caulis lonicerae was pulverized to 60 mesh sieves;
The preparation method of described galangal powder is: dry galangal was pulverized to 60 mesh sieves;
The preparation method of described radix jurineae powder is: dry radix jurineae was pulverized to 60 mesh sieves;
The preparation method of described Asian puccoon powder is: dry Asian puccoon was pulverized to 60 mesh sieves;
The preparation technology of described reinforcing agent is: (1) mixes caulis lonicerae powder, galangal powder, radix jurineae powder, Asian puccoon powder, in caulis lonicerae powder, galangal powder, radix jurineae powder, Asian puccoon powder mixture, add distilled water by solid-to-liquid ratio 1:5, at 45 DEG C, soak 2h, filter, filter residue and drying powder is crossed to 60 mesh sieves, stand-by; (2) sodium chloride, sodium butyrate, ferrous sulfate, calcium gluconae are mixed, stand-by; (3) gains in extract from pine needles, the thick polysaccharide of the leaf of bamboo, vitamin C and step (1), step (2) are mixed, obtain reinforcing agent.
In the present invention, a kind of preparation method of pig's feed complex enzyme formulation is as follows: complex enzyme is mixed and be get final product with reinforcing agent.
The addition of product of the present invention in pig feed is 800 grams/ton.
Embodiment 3
A kind of pig's feed complex enzyme formulation, is made up of complex enzyme and reinforcing agent, and the mass ratio of complex enzyme and reinforcing agent is 100:6;
Described complex enzyme is: in every gram of complex enzyme, the activity of various enzymes is as follows: lipase 1000 IU, neutral proteinase 1600IU, beta amylase 650IU, isoamylase 200IU, neutral phytase 2000IU, 'beta '-mannase 140IU, zytase 300IU, alpha-galactosidase 200IU;
Described reinforcing agent is made up of following raw materials by weight portion: 3 parts of extract from pine needles, 4 parts of the thick polysaccharide of the leaf of bamboo, 4 parts, caulis lonicerae powder, 4 parts, galangal powder, 3 parts, radix jurineae powder, 6 parts, Asian puccoon powder, 0.04 part of vitamin C, 0.06 part, sodium chloride, 0.03 part of sodium butyrate, 0.03 part, ferrous sulfate, 0.03 part of calcium gluconae;
The preparation method of described extract from pine needles is: by fresh pine needle impurity elimination, be placed in the dry 24h of vacuum drying chamber, baking temperature is 65 DEG C, and crushed after being dried is crossed 20 mesh sieves, obtains pine needle meal; By pine needle meal, cellulase and pectase in mass ratio 82:1.8:1.6 mix, in the mixture of pine needle meal, cellulase and pectase, add distilled water by solid-to-liquid ratio 1:15, at 32 DEG C, soak 48h; Immersion finishes rear filtration, and filtrate is adopted to centrifugal treating, and centrifugal rotational speed is 3600r/min, and centrifugation time is 5min, after centrifugal end, gets supernatant, by supernatant freeze drying, pulverizes 60 mesh sieves, obtains extract from pine needles;
The preparation method of the thick polysaccharide of the described leaf of bamboo: by leaf of bamboo drying and crushing, cross 30 mesh sieves, obtain bamboo leaf powder, bamboo leaf powder is mixed by 1:16 with distilled water, adopt refluxing extraction 60min, extracting temperature is 80 DEG C, extraction finishes rear filtration, by filtrate concentrate drying, pulverizes 60 mesh sieves and obtains the thick polysaccharide of the leaf of bamboo;
The preparation method of described caulis lonicerae powder is: dry caulis lonicerae was pulverized to 60 mesh sieves;
The preparation method of described galangal powder is: dry galangal was pulverized to 60 mesh sieves;
The preparation method of described radix jurineae powder is: dry radix jurineae was pulverized to 60 mesh sieves;
The preparation method of described Asian puccoon powder is: dry Asian puccoon was pulverized to 60 mesh sieves;
The preparation technology of described reinforcing agent is: (1) mixes caulis lonicerae powder, galangal powder, radix jurineae powder, Asian puccoon powder, in caulis lonicerae powder, galangal powder, radix jurineae powder, Asian puccoon powder mixture, add distilled water by solid-to-liquid ratio 1:5, at 40-50 DEG C, soak 3h, filter, filter residue and drying powder is crossed to 60 mesh sieves, stand-by; (2) sodium chloride, sodium butyrate, ferrous sulfate, calcium gluconae are mixed, stand-by; (3) gains in extract from pine needles, the thick polysaccharide of the leaf of bamboo, vitamin C and step (1), step (2) are mixed, obtain reinforcing agent.
In the present invention, a kind of preparation method of pig's feed complex enzyme formulation is as follows: complex enzyme is mixed and be get final product with reinforcing agent.
The addition of product of the present invention in pig feed is 1000 grams/ton.
Embodiment 4
A kind of pig's feed complex enzyme formulation, is made up of complex enzyme and reinforcing agent, and the mass ratio of complex enzyme and reinforcing agent is 100:1;
Described complex enzyme is: in every gram of complex enzyme, the activity of various enzymes is as follows: lipase 300IU, neutral proteinase 1800IU, beta amylase 200IU, isoamylase 50IU, neutral phytase 1000IU, 'beta '-mannase 200IU, zytase 100IU, alpha-galactosidase 50IU;
Described reinforcing agent is made up of following raw materials by weight portion: 5 parts of extract from pine needles, 1 part of the thick polysaccharide of the leaf of bamboo, 1 part, caulis lonicerae powder, 1 part, galangal powder, 6 parts, radix jurineae powder, 1 part, Asian puccoon powder, 0.01 part of vitamin C, 0.01 part, sodium chloride, 0.06 part of sodium butyrate, 0.06 part, ferrous sulfate, 0.06 part of calcium gluconae;
All the other are with embodiment 2.
Embodiment 5
A kind of pig's feed complex enzyme formulation, is made up of complex enzyme and reinforcing agent, and the mass ratio of complex enzyme and reinforcing agent is 100:5;
Described complex enzyme is: in every gram of complex enzyme, the activity of various enzymes is as follows: lipase 800 IU, neutral proteinase 1400IU, beta amylase 550 IU, isoamylase 150IU, neutral phytase 1800IU, 'beta '-mannase 120IU, zytase 250IU, alpha-galactosidase 150IU;
Described reinforcing agent is made up of following raw materials by weight portion: 1 part of extract from pine needles, 1 part of the thick polysaccharide of the leaf of bamboo, 1 part, caulis lonicerae powder, 1 part, galangal powder, 1 part, radix jurineae powder, 1 part, Asian puccoon powder, 0.08 part of vitamin C, 0.08 part, sodium chloride, 0.08 part of sodium butyrate, 0.05 part, ferrous sulfate, 0.05 part of calcium gluconae;
All the other are with embodiment 2.
Experimental example 1: to the investigation of efficiency of feed utilization and pig growing state
It is 360 of the health pig of (45.5 ± 2.1) kg that twins (group) limited company subordinate plant average weight is selected in test, is divided at random 6 groups, every group of 6 repetitions, and 10 of each repetitions, comprising a blank group and 4 test group.Blank group swine rations is basal diet; In all the other 4 test group swine rationses, add respectively the enzyme preparation that embodiment 1-5 makes; Live pig free choice feeding and drinking-water, carry out immunity and expelling parasite routinely, and observe the health status of pig, and ramming material weighing after 30 days calculates daily gain, feedstuff-meat ratio.Result of the test is in table 1.
After on-test, gather the slurry of feces and urine of each group of pig every day, air-dry its phosphorus content of rear mensuration and nitrogen content, phosphorus content in calculating mean value and fecaluria (nitrogen content) reduced rate, observe the responding time (being phosphorus in live pig fecaluria, significantly reduced time of nitrogen content) of each group, result is respectively in Table table 2 and table 3.
The each group of table 1 pig upgrowth situation table
Nitrogenous in table 2 fecaluria, phosphorus amount situation of change
| Group | The mean value of nitrogen content (%) in fecaluria | The mean value of phosphorus content (%) in fecaluria |
| Control group | 1.746 | 0.521 |
| Embodiment 1 | 1.166 | 0.291 |
| Embodiment 2 | 1.103 | 0.276 |
| Embodiment 3 | 1.156 | 0.284 |
| Embodiment 4 | 1.381 | 0.396 |
| Embodiment 5 | 1.121 | 0.281 |
The speed situation that takes effect of the each group of table 3
Can find out from above-mentioned test example, the pig's feed complex enzyme formulation that the present invention makes, can effectively improve the utilization rate of feed, reduces feedstuff-meat ratio, promotes the live pig speed of pig, and product of the present invention can also reduce the incidence of disease of pig simultaneously;
In addition can find from embodiment 1 and embodiment 5 contrasts, the reinforcing agent in the present invention can also be accelerated the reaction rate of enzyme preparation, and complex enzyme formulation takes effect faster.
Claims (5)
1. a pig's feed complex enzyme formulation, is characterized in that: be made up of complex enzyme and reinforcing agent, the mass ratio of complex enzyme and reinforcing agent is 100:3-6;
Described complex enzyme is: in every gram of complex enzyme, the activity of various enzymes is as follows: lipase 600-1000 IU, neutral proteinase 1200-1600IU, beta amylase 450-650IU, isoamylase 100-200IU, neutral phytase 1600-2000IU, 'beta '-mannase 100-140IU, zytase 200-300IU, alpha-galactosidase 100-200IU; Described reinforcing agent is made up of following raw materials by weight portion: extract from pine needles 1-3 part, the thick polysaccharide 2-4 of leaf of bamboo part, caulis lonicerae powder 2-4 part, galangal powder 2-4 part, radix jurineae powder 1-3 part, Asian puccoon powder 3-6 part, vitamin C 0.01-0.04 part, sodium chloride 0.04-0.06 part, sodium butyrate 0.01-0.03 part, ferrous sulfate 0.01-0.03 part, calcium gluconae 0.01-0.03 part;
The preparation method of described extract from pine needles is: by fresh pine needle impurity elimination, be placed in the dry 24h of vacuum drying chamber, baking temperature is 65 DEG C, and crushed after being dried is crossed 20 mesh sieves, obtains pine needle meal; By pine needle meal, cellulase and pectase in mass ratio 82:1-1.8:1-1.6 mix, in the mixture of pine needle meal, cellulase and pectase, add distilled water by solid-to-liquid ratio 1:10-15, at 32 DEG C, soak 24-48h; Immersion finishes rear filtration, and filtrate is adopted to centrifugal treating, and centrifugal rotational speed is 3600r/min, and centrifugation time is 5min, after centrifugal end, gets supernatant, by supernatant freeze drying, pulverizes 60 mesh sieves, obtains extract from pine needles;
The preparation method of the thick polysaccharide of the described leaf of bamboo: by leaf of bamboo drying and crushing, cross 30 mesh sieves, obtain bamboo leaf powder, bamboo leaf powder is mixed by 1:12-16 with distilled water, adopt refluxing extraction 40-60min, extraction temperature is 60-80 DEG C, extracts and finishes rear filtration, by filtrate concentrate drying, pulverized 60 mesh sieves and obtained the thick polysaccharide of the leaf of bamboo.
2. a kind of pig's feed complex enzyme formulation according to claim 1, is characterized in that being made up of complex enzyme and reinforcing agent, and the mass ratio of complex enzyme and reinforcing agent is 100:5;
Described complex enzyme is: in every gram of complex enzyme, the activity of various enzymes is as follows: lipase 800 IU, neutral proteinase 1400IU, beta amylase 550 IU, isoamylase 150IU, neutral phytase 1800IU, 'beta '-mannase 120IU, zytase 250IU, alpha-galactosidase 150IU;
Described reinforcing agent is made up of following raw materials by weight portion: 2 parts of extract from pine needles, 3 parts of the thick polysaccharide of the leaf of bamboo, 3 parts, caulis lonicerae powder, 3 parts, galangal powder, 2 parts, radix jurineae powder, 5 parts, Asian puccoon powder, 0.03 part of vitamin C, 0.05 part, sodium chloride, 0.02 part of sodium butyrate, 0.02 part, ferrous sulfate, 0.02 part of calcium gluconae;
The preparation method of described extract from pine needles is: by fresh pine needle impurity elimination, be placed in the dry 24h of vacuum drying chamber, baking temperature is 65 DEG C, and crushed after being dried is crossed 20 mesh sieves, obtains pine needle meal; By pine needle meal, cellulase and pectase in mass ratio 82:1.4:1.3 mix, in the mixture of pine needle meal, cellulase and pectase, add distilled water by solid-to-liquid ratio 1:13, at 32 DEG C, soak 40h; Immersion finishes rear filtration, and filtrate is adopted to centrifugal treating, and centrifugal rotational speed is 3600r/min, and centrifugation time is 5min, after centrifugal end, gets supernatant, by supernatant freeze drying, pulverizes 60 mesh sieves, obtains extract from pine needles;
The preparation method of the thick polysaccharide of the described leaf of bamboo: by leaf of bamboo drying and crushing, cross 30 mesh sieves, obtain bamboo leaf powder, bamboo leaf powder is mixed by 1:14 with distilled water, adopt refluxing extraction 50min, extracting temperature is 70 DEG C, extraction finishes rear filtration, by filtrate concentrate drying, pulverizes 60 mesh sieves and obtains the thick polysaccharide of the leaf of bamboo.
3. according to a kind of pig's feed complex enzyme formulation described in claim 1 or 2, the preparation technology who it is characterized in that reinforcing agent is: (1) mixes caulis lonicerae powder, galangal powder, radix jurineae powder, Asian puccoon powder, in caulis lonicerae powder, galangal powder, radix jurineae powder, Asian puccoon powder mixture, add distilled water by solid-to-liquid ratio 1:5, at 40-50 DEG C, soak 1-3h, filter, filter residue and drying powder is crossed to 60 mesh sieves, stand-by; (2) sodium chloride, sodium butyrate, ferrous sulfate, calcium gluconae are mixed, stand-by; (3) gains in extract from pine needles, the thick polysaccharide of the leaf of bamboo, vitamin C and step (1), step (2) are mixed, obtain reinforcing agent.
4. a kind of pig's feed complex enzyme formulation according to claim 1, is characterized in that the addition in pig feed is 600-1000 gram/ton.
5. a kind of pig's feed complex enzyme formulation according to claim 4, is characterized in that the addition in pig feed is 800 grams/ton.
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| WO2016128444A1 (en) * | 2015-02-10 | 2016-08-18 | Dsm Ip Assets B.V. | A method for improving feed digestibility and growth performance |
| CN107373143A (en) * | 2017-08-17 | 2017-11-24 | 杭州加润营科生物科技有限公司 | Young pig feed additive |
| CN108065133A (en) * | 2018-01-05 | 2018-05-25 | 浙江新篁生物技术有限公司 | A kind of forage for sea cucumber additive and its manufacturing method |
| CN108077653A (en) * | 2018-01-05 | 2018-05-29 | 浙江新篁生物技术有限公司 | A kind of seawater shrimps feed addictive and its manufacturing method |
| CN109793108A (en) * | 2018-12-25 | 2019-05-24 | 北京康宝利华生物科技有限公司 | A kind of complex enzyme formulation and its preparation method and application |
| KR102168732B1 (en) * | 2020-04-14 | 2020-10-22 | 김영아 | Method for manufacturing pig feed mixed pine needle extract |
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| WO2016128444A1 (en) * | 2015-02-10 | 2016-08-18 | Dsm Ip Assets B.V. | A method for improving feed digestibility and growth performance |
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| CN107373143B (en) * | 2017-08-17 | 2020-07-07 | 杭州加润营科生物科技有限公司 | Feed additive for piglets |
| CN108065133A (en) * | 2018-01-05 | 2018-05-25 | 浙江新篁生物技术有限公司 | A kind of forage for sea cucumber additive and its manufacturing method |
| CN108077653A (en) * | 2018-01-05 | 2018-05-29 | 浙江新篁生物技术有限公司 | A kind of seawater shrimps feed addictive and its manufacturing method |
| CN109793108A (en) * | 2018-12-25 | 2019-05-24 | 北京康宝利华生物科技有限公司 | A kind of complex enzyme formulation and its preparation method and application |
| KR102168732B1 (en) * | 2020-04-14 | 2020-10-22 | 김영아 | Method for manufacturing pig feed mixed pine needle extract |
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Effective date of registration: 20160308 Address after: 517583 Huitong Industrial Park, butterfly Ling Industrial City, Dongyuan County, Heyuan, Guangdong Patentee after: Heyuan twin Feed Co., Ltd. Address before: 330096 Nanchang high tech Zone, torch street, No. 999, twins group, Jiangxi Patentee before: Twins (Group) Co., Ltd. |