CN102860438A - Efficient pig-feed complex enzyme preparation with enzyme protectant - Google Patents
Efficient pig-feed complex enzyme preparation with enzyme protectant Download PDFInfo
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Abstract
The invention provides an efficient pig-feed complex enzyme preparation with enzyme protectant. The efficient pig-feed complex enzyme preparation with enzyme protectant is capable of promoting absorption of nutrients in the feed by pigs to increase feed utilization rate by degrading antinutritional factors, such as beta-glucan, phytic acid and pectin, biomacromolecules and the like. By adding the protectant, inactivity rate in enzymes can be reduced, effective time can be prolonged, and the effects of the enzyme preparation are more evident and longer. The efficient pig-feed complex enzyme preparation with enzyme protectant is high in feed conversion rate, feed-to-grain ratio can be lowered by more than 19%, growth speed of pigs is increased by more than 23%, and enzyme activity is kept in 18 months and can be kept by more than 90%.
Description
Technical field
The present invention relates to a kind of pig's feed complex enzyme formulation, relate in particular to a kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent.
Background technology
Enzyme is a kind of specific proteins with catalytic reaction ability, is the high effective substance that promotes biochemical reaction.Enzyme preparation is the protein with living things catalysis ability that a class is extracted from animal, plant and microorganism.Fodder enzyme preparation is a kind of take enzyme as the major function factor and the feed addictive that processes by specific production technology.Fodder enzyme preparation can promote to digest and assimilate, and reduces the pollution to environment; Reduce chymeviscosity, improve the digestibility of feed; Improve the ratio of digestible nutrient, improve food utilization efficiency; Eliminate ANFs in the feed; Improve the gut flora state, improve the animal health level.
The internal feed additive technology mostly directly adopts and pulverizes premix as feed; or when processing raw material, only add exogenous enzymes; do not add some protective agent and zymoexciter; and feed link is everlasting and is used in the open environment; exogenous enzymes is being stored and activity decreased often when feeding, affect the Forage Nutritive Value of feed itself.
Summary of the invention
The invention reside in provides a kind of efficient pig's feed complex enzyme formulation, is added with enzymatic protective reagent in this efficient pig's feed complex enzyme formulation, can reduce the inactivation of enzyme in the enzyme preparation storage process, prolongs effective time, can not affect the Forage Nutritive Value of feed itself.
The present invention is achieved through the following technical solutions:
A kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent is comprised of complex enzyme formulation and protective agent:
Various enzymes is active as follows in every gram complex enzyme formulation:
1,4 beta-glucanase 5000-6000 IU, 'beta '-mannase 1000-1500 IU, neutral phytase 600-1000 IU, pectase 2000-3000 IU, zytase 7000-8000 IU, cellulase 3000-4000 IU, neutral proteinase 2000-3000 IU, acid protease 1000-1800 IU, AMS 3000-5000 IU, carbohydrase 800-1500 IU, alpha-galactosidase 800-1200 IU;
Protectant addition is the 1%-5% of complex enzyme formulation weight.
The addition of efficient pig's feed complex enzyme formulation in the live pig daily ration that is added with enzymatic protective reagent is 1000-1500 g/ ton.
Wherein protective agent makes by the following method:
The weight portion of each raw material is in the protective agent: disodium glycyrrhizinate 15-20 part, vanillic aldehyde 10-20 part, sodium chloride 5-10 part, stevioside 5-10 part, L-rhamnose 5-10 part, maltose 1-5 part, chlorogenic acid 1-5 part, D-malic acid 2-5 part, butanedioic acid 2-5 part, sorbierite 3-5 part, potassium chloride 2-5 part, calcium carbonate 2-5 part, 2-5 parts in zinc sulfate, ferrous sulfate 1-5 part, blue stone 1-5 part, resveratrol 1-3 part, meso inositol 1-3 part;
Preparation: vanillic aldehyde, stevioside, maltose are pulverized, crossed in the steam that the 40-60 mesh sieve is placed on 90-100 ℃ and processed 5-10 minute, then be cooled to rapidly 15-25 ℃, left standstill 1-2 hour; Sorbierite, resveratrol are pulverized, cross that to add mass fraction behind the 40-60 mesh sieve be ethanol 200-300 part of 80-95%, then under 30-40 ℃, the maintenance supersonic frequency is 200-300W, filter after ultrasonic 50-80 minute, get filtrate and ethanol is removed in heating, then-10 ℃ to-20 ℃ lower freezing 10-15 hours, vacuum drying 3-5 is individual hour again; Disodium glycyrrhizinate in the protective agent prescription, sodium chloride, L-rhamnose, chlorogenic acid, D-malic acid, butanedioic acid, potassium chloride, calcium carbonate, zinc sulfate, ferrous sulfate, blue stone, meso inositol are pulverized; cross the 40-60 mesh sieve, again with process after vanillic aldehyde, stevioside, maltose and sorbierite and resveratrol mixing get final product.
Umber described in protective agent prescription and the preparation method is for being weight portion.
The ANFs such as the beta glucan in 1,4 beta-glucanase, neutral phytase, the pectase degradable feed, phytic acid, pectin, the materials such as the polysaccharide in zytase, cellulase, neutral proteinase, acid protease, AMS and the carbohydrase degradable feed and large biological molecule, thereby promote pig to the absorption of nutritional labeling in the feed, improve efficiency of feed utilization; Protectant adding can reduce the inactivation rate of enzyme, prolongs effective time, makes the effect of this enzyme preparation more significantly with lasting; The materials such as the sodium chloride in the protective agent, calcium carbonate, zinc sulfate, meso inositol not only can protective enzyme activity, also can be pig provides growth required mineral element and nutriment.
The high efficiency composition enzyme preparation feed conversion rate that the present invention makes is high, can use feedstuff-meat ratio to descend more than 19%; The pig speed of growth improves more than 23%; The enzymatic activity retention time is long, and in 18 months, enzymatic activity can remain on more than 90%.
The specific embodiment:
Embodiment 1:
A kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent is comprised of complex enzyme formulation and protective agent:
Various enzymes is active as follows in every gram complex enzyme formulation:
1,4 beta-glucanase 5500 IU, 'beta '-mannase 1300 IU, neutral phytase 800 IU, pectase 2550 IU, zytase 7560 IU, cellulase 3500 IU, neutral proteinase 2500 IU, acid protease 1440 IU, AMS 4000 IU, carbohydrase 1180 IU, alpha-galactosidase 1000 IU;
Protectant addition is 3% of complex enzyme formulation weight.
The addition of efficient pig's feed complex enzyme formulation in the live pig daily ration that is added with enzymatic protective reagent is 1200 g/ tons.
Wherein protective agent makes by the following method:
The weight portion of each raw material is in the protective agent: 18 parts of disodium glycyrrhizinates, 18 parts of vanillic aldehydes, 8 parts in sodium chloride, 8 parts of steviosides, 8 parts of L-rhamnoses, 3 parts of maltose, 3 parts of chlorogenic acids, 4 parts of D-malic acids, 4 parts of butanedioic acids, 4 parts of sorbierites, 4 parts in potassium chloride, 4 parts in calcium carbonate, 4 parts in zinc sulfate, 3 parts in ferrous sulfate, 3 parts in blue stone, 2 parts of resveratrols, 2 parts of meso inositols;
Preparation: vanillic aldehyde, stevioside, maltose are pulverized, crossed in the steam that 50 mesh sieves are placed on 95 ℃ and processed 8 minutes, then be cooled to rapidly 20 ℃, left standstill 1.5 hours; Sorbierite, resveratrol are pulverized, crossed that to add mass fraction behind 50 mesh sieves be 250 parts of 85% ethanol, then under 35 ℃, the maintenance supersonic frequency is 250W, and filtrate is got in filtration after ultrasonic 60 minutes and ethanol is removed in heating, then descended freezing 12 hours at-15 ℃, vacuum drying is 4 hours again; Disodium glycyrrhizinate in the protective agent prescription, sodium chloride, L-rhamnose, chlorogenic acid, D-malic acid, butanedioic acid, potassium chloride, calcium carbonate, zinc sulfate, ferrous sulfate, blue stone, meso inositol are pulverized, cross 50 mesh sieves, again with process after vanillic aldehyde, stevioside, maltose and sorbierite and resveratrol mixing get final product;
Embodiment 2:
A kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent is comprised of complex enzyme formulation and protective agent:
Various enzymes is active as follows in every gram complex enzyme formulation:
1,4 beta-glucanase 5000 IU, 'beta '-mannase 1000 IU, neutral phytase 600 IU, pectase 2000 IU, zytase 7000 IU, cellulase 3000 IU, neutral proteinase 2000 IU, acid protease 1000 IU, AMS 3000 IU, carbohydrase 800 IU, alpha-galactosidase 800 IU;
Protectant addition is 1% of complex enzyme formulation weight.
The addition of efficient pig's feed complex enzyme formulation in the live pig daily ration that is added with enzymatic protective reagent is 1000 g/ tons.
Wherein protective agent makes by the following method:
The weight portion of each raw material is in the protective agent: 15 parts of disodium glycyrrhizinates, 10 parts of vanillic aldehydes, 5 parts in sodium chloride, 5 parts of steviosides, 5 parts of L-rhamnoses, 1 part of maltose, 1 part of chlorogenic acid, 2 parts of D-malic acids, 2 parts of butanedioic acids, 3 parts of sorbierites, 2 parts in potassium chloride, 2 parts in calcium carbonate, 2 parts in zinc sulfate, 1 part in ferrous sulfate, 1 part in blue stone, 1 part of resveratrol, 1 part of meso inositol;
Preparation: vanillic aldehyde, stevioside, maltose are pulverized, crossed in the steam that 40 mesh sieves are placed on 90 ℃ and processed 5 minutes, then be cooled to rapidly 15 ℃, left standstill 1 hour; Sorbierite, resveratrol are pulverized, crossed that to add mass fraction behind 40 mesh sieves be 200 parts of 80% ethanol, then under 30 ℃, the maintenance supersonic frequency is 200W, and filtrate is got in filtration after ultrasonic 50 minutes and ethanol is removed in heating, then descended freezing 10 hours at-10 ℃, vacuum drying is 3 hours again; Disodium glycyrrhizinate in the protective agent prescription, sodium chloride, L-rhamnose, chlorogenic acid, D-malic acid, butanedioic acid, potassium chloride, calcium carbonate, zinc sulfate, ferrous sulfate, blue stone, meso inositol are pulverized; cross 40 mesh sieves, again with process after vanillic aldehyde, stevioside, maltose and sorbierite and resveratrol mixing get final product.
Embodiment 3:
A kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent is comprised of complex enzyme formulation and protective agent:
Various enzymes is active as follows in every gram complex enzyme formulation:
1,4 beta-glucanase 6000 IU, 'beta '-mannase 1500 IU, neutral phytase 1000 IU, pectase 3000 IU, zytase 8000 IU, cellulase 4000 IU, neutral proteinase 3000 IU, acid protease 1800 IU, AMS 5000 IU, carbohydrase 1500 IU, alpha-galactosidase 1200 IU;
Protectant addition is 5% of complex enzyme formulation weight.
The addition of efficient pig's feed complex enzyme formulation in the live pig daily ration that is added with enzymatic protective reagent is 1500 g/ tons.
Wherein protective agent makes by the following method:
The weight portion of each raw material is in the protective agent: 20 parts of disodium glycyrrhizinates, 20 parts of vanillic aldehydes, 10 parts in sodium chloride, 10 parts of steviosides, 10 parts of L-rhamnoses, 5 parts of maltose, 5 parts of chlorogenic acids, 5 parts of D-malic acids, 5 parts of butanedioic acids, 5 parts of sorbierites, 5 parts in potassium chloride, 5 parts in calcium carbonate, 5 parts in zinc sulfate, 5 parts in ferrous sulfate, 5 parts in blue stone, 3 parts of resveratrols, 3 parts of meso inositols;
Preparation: vanillic aldehyde, stevioside, maltose are pulverized, crossed in the steam that 60 mesh sieves are placed on 100 ℃ and processed 10 minutes, then be cooled to rapidly 25 ℃, left standstill 2 hours; Sorbierite, resveratrol are pulverized, crossed that to add mass fraction behind 60 mesh sieves be 300 parts of 95% ethanol, then under 40 ℃, the maintenance supersonic frequency is 300W, and filtrate is got in filtration after ultrasonic 80 minutes and ethanol is removed in heating, then descended freezing 15 hours at-20 ℃, vacuum drying is 5 hours again; Disodium glycyrrhizinate in the protective agent prescription, sodium chloride, L-rhamnose, chlorogenic acid, D-malic acid, butanedioic acid, potassium chloride, calcium carbonate, zinc sulfate, ferrous sulfate, blue stone, meso inositol are pulverized; cross 60 mesh sieves, again with process after vanillic aldehyde, stevioside, maltose and sorbierite and resveratrol mixing get final product.
Embodiment 4:
A kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent is comprised of complex enzyme formulation and protective agent:
Various enzymes is active as follows in every gram complex enzyme formulation:
1,4 beta-glucanase 4000 IU, 'beta '-mannase 2000 IU, neutral phytase 500 IU, pectase 1500 IU, zytase 9000 IU, cellulase 5000 IU, neutral proteinase 4000 IU, acid protease 2000 IU, AMS 2000 IU, carbohydrase 700 IU, alpha-galactosidase 600 IU;
Protectant addition is 6% of complex enzyme formulation weight.
The addition of efficient pig's feed complex enzyme formulation in the live pig daily ration that is added with enzymatic protective reagent is 900 g/ tons.
Wherein protective agent makes by the following method:
The weight portion of each raw material is in the protective agent: 23 parts of disodium glycyrrhizinates, 8 parts of vanillic aldehydes, 3 parts in sodium chloride, 14 parts of steviosides, 2 parts of L-rhamnoses, 8 parts of maltose, 7 parts of chlorogenic acids, 1 part of D-malic acid, 7 parts of butanedioic acids, 1 part of sorbierite, 1 part in potassium chloride, 8 parts in calcium carbonate, 6 parts in zinc sulfate, 0.5 part in ferrous sulfate, 0.5 part in blue stone, 5 parts of resveratrols, 5 parts of meso inositols;
Preparation: vanillic aldehyde, stevioside, maltose are pulverized, crossed in the steam that 80 mesh sieves are placed on 80 ℃ and processed 3 minutes, then be cooled to rapidly 30 ℃, left standstill 3 hours; Sorbierite, resveratrol are pulverized, crossed that to add mass fraction behind 80 mesh sieves be 400 parts of 70% ethanol, then under 20 ℃, the maintenance supersonic frequency is 350W, and filtrate is got in filtration after ultrasonic 90 minutes and ethanol is removed in heating, then descended freezing 20 hours at-30 ℃, vacuum drying is 6 hours again; Disodium glycyrrhizinate in the protective agent prescription, sodium chloride, L-rhamnose, chlorogenic acid, D-malic acid, butanedioic acid, potassium chloride, calcium carbonate, zinc sulfate, ferrous sulfate, blue stone, meso inositol are pulverized; cross 100 mesh sieves, again with process after vanillic aldehyde, stevioside, maltose and sorbierite and resveratrol mixing get final product.
Embodiment 5:
A kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent is comprised of complex enzyme formulation and protective agent:
Various enzymes is active as follows in every gram complex enzyme formulation:
1,4 beta-glucanase 7000 IU, 'beta '-mannase 900 IU, neutral phytase 500 IU, pectase 3500 IU, zytase 5000 IU, cellulase 2500 IU, neutral proteinase 1500 IU, acid protease 2000 IU, AMS 6000 IU, carbohydrase 2500 IU, alpha-galactosidase 1500 IU.
All the other are with embodiment 1.
Embodiment6:
A kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent is comprised of complex enzyme formulation and protective agent:
Wherein protective agent makes by the following method:
The weight portion of each raw material is in the protective agent: 12 parts of disodium glycyrrhizinates, 23 parts of vanillic aldehydes, 12 parts in sodium chloride, 3 parts of steviosides, 13 parts of L-rhamnoses, 0.5 part of maltose, 0.5 part of chlorogenic acid, 9 parts of D-malic acids, 1 part of butanedioic acid, 1 part of sorbierite, 8 parts in potassium chloride, 9 parts in calcium carbonate, 0.5 part in zinc sulfate, 0.5 part in ferrous sulfate, 0.5 part in blue stone, 6 parts of resveratrols, 0.5 part of meso inositol;
Preparation: vanillic aldehyde, stevioside, maltose are pulverized, crossed in the steam that 100 mesh sieves are placed on 110 ℃ and processed 12 minutes, then be cooled to rapidly 12 ℃, left standstill 0.5 hour; Sorbierite, resveratrol are pulverized, crossed that to add mass fraction behind 80 mesh sieves be 500 parts of 98% ethanol, then under 45 ℃, the maintenance supersonic frequency is 400W, and filtrate is got in filtration after ultrasonic 45 minutes and ethanol is removed in heating, then descended freezing 18 hours at-5 ℃, vacuum drying is 2 hours again; Disodium glycyrrhizinate in the protective agent prescription, sodium chloride, L-rhamnose, chlorogenic acid, D-malic acid, butanedioic acid, potassium chloride, calcium carbonate, zinc sulfate, ferrous sulfate, blue stone, meso inositol are pulverized; cross 100 mesh sieves, again with process after vanillic aldehyde, stevioside, maltose and sorbierite and resveratrol mixing get final product.
All the other are with embodiment 1.
Embodiment 7:
Be the disclosed a kind of complex enzyme preparation for feeding piglets of Chinese patent of CN 102119768A according to publication number, in every gram complex enzyme preparation for feeding piglets, the enzyme activity of acid protease is that 15000IU, diastatic enzyme activity are that the enzyme activity of 15000IU, zytase is that the enzyme activity of 120000IU, 1,4 beta-glucanase is that the enzyme activity of 50000IU, cellulase is that the enzyme activity of 15000IU, pectase is 8000IU.In the piglet diet feed, add 0.15% this complex enzyme formulation.
Test 1: to the investigation of efficiency of feed utilization and pig growing state
It is 320 of the hampshire health pig of (36.0 ± 3.4) kg that subordinate plant of Dongguan City twins feed corporation,Ltd average weight is selected in test, is divided at random 8 groups, every group of 4 repetitions, and 10 of each repetitions are comprising a blank group and 7 test group.Do not add any enzyme preparation in the blank group swine rations; Added respectively the enzyme preparation that embodiment 1-7 makes in all the other 7 test group swine rationses; Live pig free choice feeding and drinking-water carry out immunity and expelling parasite routinely, record the daily ingestion amount of every group of test pig, and ramming material and weighing after 30 days calculates daily gain, feed intake, feedstuff-meat ratio (daily ingestion amount/daily gain), speed of growth increase rate and feedstuff-meat ratio rate of descent.Result of the test sees Table 1 and table 2.
Speed of growth increase rate=(test group daily gain/control group daily gain-1) * 100%
Feedstuff-meat ratio rate of descent=(1-test group feedstuff-meat ratio/control group feedstuff-meat ratio) * 100%
Table 1 is respectively organized speed of growth increase rate
Table 2 is respectively organized the feedstuff-meat ratio rate of descent
Test 2: the investigation of enzymatic activity
The product that embodiment 1-7 makes is deposited in the incubator, and keeping temperature is 20 ± 5 ℃, and humidity is 45 ± 5%.Zytase, 1,4 beta-glucanase and cellulose enzyme activity in each product that mensuration embodiment 1-7 makes during respectively at 0,6,12,18 month.
The mensuration of cellulase activity (DNS method)
Get the enzyme liquid (through 40 ℃ balances) of 2.00 ml through suitably diluting, add 5.0 ml DNS reagent, vibration 5s.Then add 2.0 ml carboxymethylcellulose sodium solutions (through 40 ℃ of balances), 40 ℃ of insulation 30 min, boiling water bath heats 5 min.Be settled to 25 ml with distilled water after being cooled to room temperature, vibration 5s.Take the blank sample of standard as blank, measure absorbance at 540 nm places.
Get the enzyme liquid (through 40 ℃ balances) of 2.00 ml through suitably diluting, add 2.0 ml carboxymethylcellulose sodium solutions (through 40 ℃ of balances), vibration 5s is in 40 ℃ of insulation 30min.Add 5.0 ml DNS reagent, vibration 5s.Boiling water bath heating 5min is settled to 25 ml with distilled water, electromagnetic viscosimeter 5s after being cooled to room temperature.Take the blank sample of standard as blank, measure absorbance at 540 nm places.
Calculate the cellulase activity value, the results are shown in Table 3.
The mensuration of Xylanase activity also adopts the DNS method, the results are shown in Table 4.
The mensuration of activity of beta-glucanase also adopts the DNS method, the results are shown in Table 5.
Table 3 cellulase activity detection case
Table 4 Xylanase activity detection case
Table 5 activity of beta-glucanase detection case
Can find out from above-mentioned test example, the feedstuff-meat ratio rate of descent all reaches more than 19% in test group corresponding to embodiment 1-3, speed of growth increase rate is all greater than 23%, enzymatic activity all remains on more than 90% in 18 months, far above embodiment 4-7 corresponding test group and control group, the wherein best results of the test group of embodiment 1 correspondence.So the present invention can improve the utilization rate of feed, promote the growth of pig, and the enzymatic activity maintenance better, is a kind of desirable pig feed additive.
Claims (9)
1. an efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent is characterized in that: be comprised of complex enzyme formulation and protective agent.
2. a kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent as claimed in claim 1, it is characterized in that: the addition in the live pig daily ration is 1000-1500 g/ ton; Protectant addition is the 1%-5% of complex enzyme formulation weight.
3. a kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent as claimed in claim 2, it is characterized in that: the addition in the live pig daily ration is 1200 g/ tons; Protectant addition is 3% of complex enzyme formulation weight.
4. a kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent as claimed in claim 1; it is characterized in that: various enzymes is active as follows in every gram complex enzyme formulation: 1,4 beta-glucanase 5000-6000 IU; 'beta '-mannase 1000-1500 IU; neutral phytase 600-1000 IU; pectase 2000-3000 IU; zytase 7000-8000 IU; cellulase 3000-4000 IU; neutral proteinase 2000-3000 IU; acid protease 1000-1800 IU; AMS 3000-5000 IU, carbohydrase 800-1500 IU, alpha-galactosidase 800-1200 IU.
5. a kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent as claimed in claim 4; it is characterized in that: various enzymes is active as follows in every gram complex enzyme formulation: 1,4 beta-glucanase 5500 IU, 'beta '-mannase 1300 IU, neutral phytase 800 IU; pectase 2550 IU; zytase 7560 IU, cellulase 3500 IU, neutral proteinase 2500 IU; acid protease 1440IU; AMS 4000 IU, carbohydrase 1180 IU, alpha-galactosidase 1000 IU.
6. a kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent as claimed in claim 1; it is characterized in that: the weight portion of each raw material is in the protective agent: disodium glycyrrhizinate 15-20 part; vanillic aldehyde 10-15 part; sodium chloride 5-10 part, stevioside 5-10 part, L-rhamnose 5-10 part; maltose 1-5 part; chlorogenic acid 1-5 part, D-malic acid 2-5 part, butanedioic acid 2-5 part; sorbierite 3-5 part; potassium chloride 2-5 part, calcium carbonate 2-5 part, zinc sulfate 2-5 part; ferrous sulfate 1-5 part; blue stone 1-5 part, resveratrol 1-3 part, meso inositol 1-3 part.
7. a kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent as claimed in claim 6, it is characterized in that: the weight portion of each raw material is in the protective agent: 18 parts of disodium glycyrrhizinates, 18 parts of vanillic aldehydes; 8 parts in sodium chloride, 8 parts of steviosides, 8 parts of L-rhamnoses; 3 parts of maltose, 3 parts of chlorogenic acids, 4 parts of D-malic acids; 4 parts of butanedioic acids, 4 parts of sorbierites, 4 parts in potassium chloride; 4 parts in calcium carbonate, 4 parts in zinc sulfate, 3 parts in ferrous sulfate; 3 parts in blue stone, 2 parts of resveratrols, 2 parts of meso inositols.
8. a kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent as claimed in claim 1, it is characterized in that: protectant preparation method is as follows: vanillic aldehyde, stevioside, maltose are pulverized, cross in the steam that the 40-60 mesh sieve is placed on 90-100 ℃ and processed 5-10 minute, then be cooled to rapidly 15-25 ℃, left standstill 1-2 hour; Sorbierite, resveratrol are pulverized, cross that to add mass fraction behind the 40-60 mesh sieve be ethanol 200-300 part of 80-95%, then under 30-40 ℃, the maintenance supersonic frequency is 200-300W, filter after ultrasonic 50-80 minute, get filtrate and ethanol is removed in heating, then-10 ℃ to-20 ℃ lower freezing 10-15 hours, vacuum drying 3-5 is individual hour again; Disodium glycyrrhizinate in the protective agent prescription, sodium chloride, L-rhamnose, chlorogenic acid, D-malic acid, butanedioic acid, potassium chloride, calcium carbonate, zinc sulfate, ferrous sulfate, blue stone, meso inositol are pulverized; cross the 40-60 mesh sieve, again with process after vanillic aldehyde, stevioside, maltose and sorbierite and resveratrol mixing get final product.
9. a kind of efficient pig's feed complex enzyme formulation that is added with enzymatic protective reagent as claimed in claim 8, it is characterized in that: protectant preparation method is as follows: vanillic aldehyde, stevioside, maltose are pulverized, cross in the steam that 50 mesh sieves are placed on 95 ℃ and processed 8 minutes, then be cooled to rapidly 20 ℃, left standstill 1.5 hours; Sorbierite, resveratrol are pulverized, and 250 parts of the ethanol of excessively adding 85% behind 50 mesh sieves are then under 35 ℃, the maintenance supersonic frequency is 250W, and filtrate is got in filtration after ultrasonic 60 minutes and ethanol is removed in heating, then descended freezing 12 hours at-15 ℃, vacuum drying is 4 hours again; Disodium glycyrrhizinate in the protective agent prescription, sodium chloride, L-rhamnose, chlorogenic acid, D-malic acid, butanedioic acid, potassium chloride, calcium carbonate, zinc sulfate, ferrous sulfate, blue stone, meso inositol are pulverized; cross 50 mesh sieves, again with process after vanillic aldehyde, stevioside, maltose and sorbierite and resveratrol mixing get final product.
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