CN103998969A - 用于无标记高对比度细胞成像的定量相位显微镜 - Google Patents

用于无标记高对比度细胞成像的定量相位显微镜 Download PDF

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Publication number
CN103998969A
CN103998969A CN201280063329.1A CN201280063329A CN103998969A CN 103998969 A CN103998969 A CN 103998969A CN 201280063329 A CN201280063329 A CN 201280063329A CN 103998969 A CN103998969 A CN 103998969A
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CN
China
Prior art keywords
phase
quantitative
bundle
phase shift
diffracted beam
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Pending
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CN201280063329.1A
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English (en)
Chinese (zh)
Inventor
R.菲尔金斯
C.朱
E.M.金
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General Electric Co
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General Electric Co
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Publication of CN103998969A publication Critical patent/CN103998969A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/14—Condensers affording illumination for phase-contrast observation
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/0005—Adaptation of holography to specific applications
    • G03H2001/005—Adaptation of holography to specific applications in microscopy, e.g. digital holographic microscope [DHM]
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
    • G03H2001/0447—In-line recording arrangement
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
    • G03H2001/0452—Digital holography, i.e. recording holograms with digital recording means arranged to record an image of the object
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
    • G03H2001/0454—Arrangement for recovering hologram complex amplitude
    • G03H2001/0458—Temporal or spatial phase shifting, e.g. parallel phase shifting method
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H2223/00—Optical components
    • G03H2223/12—Amplitude mask, e.g. diaphragm, Louver filter
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H2223/00—Optical components
    • G03H2223/50—Particular location or purpose of optical element
    • G03H2223/52—Filtering the object information

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  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Multimedia (AREA)
  • Computing Systems (AREA)
  • Theoretical Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
CN201280063329.1A 2011-12-22 2012-12-20 用于无标记高对比度细胞成像的定量相位显微镜 Pending CN103998969A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/335,748 2011-12-22
US13/335,748 US8693000B2 (en) 2011-12-22 2011-12-22 Quantitative phase microscopy for label-free high-contrast cell imaging
PCT/SE2012/051445 WO2013095282A2 (en) 2011-12-22 2012-12-20 Quantitative phase microscopy for label-free high-contrast cell imaging

Publications (1)

Publication Number Publication Date
CN103998969A true CN103998969A (zh) 2014-08-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280063329.1A Pending CN103998969A (zh) 2011-12-22 2012-12-20 用于无标记高对比度细胞成像的定量相位显微镜

Country Status (5)

Country Link
US (1) US8693000B2 (enExample)
EP (1) EP2795389A4 (enExample)
JP (1) JP6208681B2 (enExample)
CN (1) CN103998969A (enExample)
WO (1) WO2013095282A2 (enExample)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107209000A (zh) * 2015-01-30 2017-09-26 浜松光子学株式会社 干涉观察装置以及干涉观察方法
CN108510501A (zh) * 2017-02-28 2018-09-07 株式会社岛津制作所 细胞观察装置
CN110376867A (zh) * 2019-06-25 2019-10-25 北京理工大学 一种高时空分辨率的离轴数字全息显微成像系统及方法
CN110709749A (zh) * 2017-06-09 2020-01-17 电子慕泽雷帕里公司 组合式明视场和相衬显微镜系统及配备其的图像处理设备
CN110907403A (zh) * 2019-11-18 2020-03-24 中国科学技术大学 单次直接定量相位成像的实现装置
CN113804625A (zh) * 2021-09-30 2021-12-17 常州北邮新一代信息技术研究院有限公司 自动跟踪细胞成像方法及系统

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JP2627727B2 (ja) * 1994-08-03 1997-07-09 株式会社中国医食研究所 骨髄品の製法
US8934103B2 (en) 2011-12-22 2015-01-13 General Electric Company Quantitative phase microscopy for label-free high-contrast cell imaging
WO2014070082A1 (en) * 2012-10-29 2014-05-08 General Electric Company Quantitative phase microscopy for label-free high-contrast cell imaging
JP6182005B2 (ja) * 2013-07-22 2017-08-16 アストロデザイン株式会社 光学的分解能向上装置
JP6195373B2 (ja) * 2013-12-19 2017-09-13 株式会社Ｓｃｒｅｅｎホールディングス 撮像装置および撮像方法
DE112015006081T5 (de) 2015-01-30 2017-11-02 Hamamatsu Photonics K.K. Interferenz-optikvorrichtung, interferenz-beobachtungsvorrichtung und interferenz-beobachtungsverfahren
CN107850530B (zh) 2015-05-04 2020-11-06 港大科桥有限公司 用于对样本进行光学成像的装置和方法
EP3115766A1 (en) 2015-07-10 2017-01-11 3Scan Inc. Spatial multiplexing of histological stains
JPWO2018158946A1 (ja) * 2017-03-03 2019-11-21 株式会社島津製作所 細胞観察装置
JP7158220B2 (ja) * 2018-09-11 2022-10-21 浜松ホトニクス株式会社 測定装置および測定方法
CN114324245B (zh) * 2021-11-15 2024-01-30 西安电子科技大学 基于部分相干结构光照明的定量相位显微装置和方法
CN116908217B (zh) * 2023-09-11 2023-11-17 中北大学 一种深孔测量与三维重建系统及其使用方法

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US3658405A (en) * 1969-05-26 1972-04-25 Maksvmilian Pluta Variable phase-contrast and interference microscope
US5241364A (en) * 1990-10-19 1993-08-31 Fuji Photo Film Co., Ltd. Confocal scanning type of phase contrast microscope and scanning microscope
US5260569A (en) * 1991-07-25 1993-11-09 Fuji Photo Film Co., Ltd. Scanning microscope and scanning mechanism
US20050099682A1 (en) * 2000-11-06 2005-05-12 Vincent Lauer Microscope for diffracting objects
WO2011042442A1 (en) * 2009-10-08 2011-04-14 Universite Libre De Bruxelles Off-axis interferometer

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US5751475A (en) * 1993-12-17 1998-05-12 Olympus Optical Co., Ltd. Phase contrast microscope
US7151632B2 (en) * 2001-01-12 2006-12-19 University Of Rochester Apparatus for production of an inhomogeneously polarized optical beam for use in illumination and a method thereof
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JP4512822B2 (ja) * 2004-10-20 2010-07-28 国立大学法人 筑波大学 線集光型フーリエドメイン干渉形状計測装置
JP2006250849A (ja) * 2005-03-14 2006-09-21 Naohiro Tanno 光コヒーレンストモグラフィー装置を用いた光画像計測方法及びその装置
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US3658405A (en) * 1969-05-26 1972-04-25 Maksvmilian Pluta Variable phase-contrast and interference microscope
US5241364A (en) * 1990-10-19 1993-08-31 Fuji Photo Film Co., Ltd. Confocal scanning type of phase contrast microscope and scanning microscope
US5260569A (en) * 1991-07-25 1993-11-09 Fuji Photo Film Co., Ltd. Scanning microscope and scanning mechanism
US20050099682A1 (en) * 2000-11-06 2005-05-12 Vincent Lauer Microscope for diffracting objects
WO2011042442A1 (en) * 2009-10-08 2011-04-14 Universite Libre De Bruxelles Off-axis interferometer

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107209000A (zh) * 2015-01-30 2017-09-26 浜松光子学株式会社 干涉观察装置以及干涉观察方法
US10393500B2 (en) 2015-01-30 2019-08-27 Hamamatsu Photonics K.K. Interference observation device and interference observation method
CN108510501A (zh) * 2017-02-28 2018-09-07 株式会社岛津制作所 细胞观察装置
CN110709749A (zh) * 2017-06-09 2020-01-17 电子慕泽雷帕里公司 组合式明视场和相衬显微镜系统及配备其的图像处理设备
CN110709749B (zh) * 2017-06-09 2021-10-26 电子慕泽雷帕里公司 组合式明视场和相衬显微镜系统及配备其的图像处理设备
CN110376867A (zh) * 2019-06-25 2019-10-25 北京理工大学 一种高时空分辨率的离轴数字全息显微成像系统及方法
CN110907403A (zh) * 2019-11-18 2020-03-24 中国科学技术大学 单次直接定量相位成像的实现装置
CN110907403B (zh) * 2019-11-18 2021-07-09 中国科学技术大学 单次直接定量相位成像的实现装置
CN113804625A (zh) * 2021-09-30 2021-12-17 常州北邮新一代信息技术研究院有限公司 自动跟踪细胞成像方法及系统

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Publication number Publication date
EP2795389A4 (en) 2015-08-12
JP2015503128A (ja) 2015-01-29
US8693000B2 (en) 2014-04-08
US20130163002A1 (en) 2013-06-27
EP2795389A2 (en) 2014-10-29
JP6208681B2 (ja) 2017-10-04
WO2013095282A2 (en) 2013-06-27
WO2013095282A3 (en) 2013-08-22

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Application publication date: 20140820