CN103989011A - Concentrate supplement for enhancing movement performance and oxidation resistance of endurance horses - Google Patents

Abstract

The invention provides a concentrate supplement for enhancing movement performance and oxidation resistance of endurance horses. The concentrate supplement comprises following raw materials: 8-83 parts of corn, 0.5-55 parts of bran, 0.5-40.5 parts of wheat middling, 1-40 parts of soybean meal, 0.4-15 parts of calcium hydrophosphate, 0.1-20 parts of table salt and 0.1-16 parts of a premix material, and is prepared into particles having a size of 3.5-4.5 mm to be fed to the horses. The premix material comprises 0.01-5 parts of cobalt chloride, 0.2-8 parts of copper sulfate, 0.05-8 parts of potassium iodide, 0.5-15 parts of ferrous sulfate, 1-20 parts of manganese sulfate, 0.5-5 parts of sodium selenite, 0.5-20 parts of zinc sulfate, 0.01-0.8 part of vitamin A, 0.005-0.5 part of vitamin D, 1-35 parts of vitamin E, 0.05-5 parts of thiamine, 0.01-1 part of riboflavin, 0.01-1 part of niacin, 0.01-1 part of pantothenic acid, 10-90 parts of thioctic acid, 0.001-0.05 part of vitamin B6, 0.01-4 parts of biotin, 0.05-6 parts of vitamin C, 0.1-6 parts of choline chloride and 30-80 parts of bentonite. The raw materials are uniformly mixed and each 0.25-4 kg of the concentrate supplement is fed to one horse per day.

Description

A kind of concentrate supplement that improves endurance horse exercise performance and oxidation resistance

Technical field

The present invention relates to endurance horse fine fodder processing, the antioxidant that especially preparation filters out, improves antioxygenic property and the games results of horse over sixty years of age here, makes endurance horse be able to fast quick-recovery at post-games physiological function.

Background technology

The research of resisting kinetic fatigue is very active in recent years, exercise induced fatigue refers to that the change due to organism physiology biochemistry in motion process causes the temporarily-depressed phenomenon of locomitivity, numerous studies confirm that while moving, when the clearance rate of interior free yl is less than endogenous free radical production rate, cause free radical to be piled up in vivo, the fast quick-recovery that affects athletic locomitivity and the rear body of motion, in body, main free radical has peroxide (O ₂-), hydrogen peroxide (H ₂o ₂), hydroxy radical (OH) etc.In the training and match of endurance horse, be prone to exercise induced fatigue, especially when body is subject to strenuous exercise's stimulation, interior free yl increases, cause body active oxygen to pile up, will be oxidized body unrighted acid, cause response to oxidative stress, cause competitiveness to decline and affect sports achievement, therefore, competition term endurance horse Nutrition and Metabolism is regulated and controled, research and development endurance horse antifatigue, decompose rapidly and get rid of the energetic supersession product after endurance horse high-intensity exercise, fast, rationally, scientifically slow down its fatigue symptom, thereby fast its competitive state of quick-recovery, significant for the exercise performance and the oxidation resistance that improve horse over sixty years of age here.

Literature search discloses: 1. application number is the patent of a CN201210297827.4 < < horse feed and preparation method thereof > >, and the weight portion that discloses raw material forms: oat and/or barley 25-60 part, corn 10-35 part, dregs of beans 3-10 part, wheat bran 5-25 part, beet pulp 3-15 part, trace element and/or mineral matter 0.3-3 part, salt 0.3-3 part, molasses 4-12 part, oily certain herbaceous plants with big flowers 0-20 part; This feed have be of high nutritive value, abundant and balanced, cost is low, good palatability, nutrient digestion rate is higher, do not increase horses rear intestinal burden and reduced the incidence of disease of horse, increased tolerance and the endurance of sports horses simultaneously yet, be applicable to various types of horses.2. application number is the weight portion composition that CN201210087824.8 < < horse feed pre-mixing material > > discloses raw material: B B-complex 3-4 part, composite mineral matter 0.5-1 part, enzyme 1-2 part, rumen-bypass amino acid 2-3 part, carrier 10-15 part, and described carrier is mushroom powder or glossy ganoderma powder; When feeding horse, add horse feed pre-mixing material of the present invention, be conducive to the growth of rumen microorganism, reduce the cud incidence of disease; It is carrier that mushroom powder or glossy ganoderma powder are take in the present invention, and the nutrition of having enriched premix forms, and has improved the mouthfeel of premix.

It is required that mixed ration formula of the present invention meets the nutrition of horse over sixty years of age here, improve the antioxygenic property of horse over sixty years of age here, metabolite after playing fast decoupled simultaneously and getting rid of horse high-intensity exercise over sixty years of age here, science, reasonably slow down its fatigue symptom, thereby fast its competitive state of quick-recovery, has important production practical value.

Summary of the invention

The object of the invention is to: filter out the optimum addn amount of antioxidant, reached and eliminated the fatigue here over sixty years of age, horse during movement produced, make endurance horse at post-games physiological function, be able to the object of fast quick-recovery.

The object of the present invention is achieved like this: a kind of concentrate supplement that improves endurance horse exercise performance and oxidation resistance, and raw material is prepared with parts by weight;

Raw material components: corn 8-83 part, wheat bran 0.5-55 part, inferior powder 0.5-40.5 part, Soybean Meal 1-40 part, calcium monohydrogen phosphate 0.4-15 part, salt 0.1-20 part, premix 0.1-16 part; Its raw material is placed in to a system machine successively, makes the particle that diameter is 3.5-4.5mm;

Wherein premix is obtained by following two kinds of formulas:

1) premix is by cobalt chloride 0.01-5 part, copper sulphate 0.2-8 part, KI 0.05-8 part, ferrous sulfate 0.5-15 part, manganese sulfate 1-20 part, sodium selenite 0.05-5 part, zinc sulfate 0.5-20 part, vitamin A 0.01-0.8 part, vitamin D 0.005-0.5 part, vitamin e1-35 part, thiamine 0.05-5 part, riboflavin 0.01-1 part, nicotinic acid 0.01-1 part, pantothenic acid 0.01-1 part, Cobastab ₆0.001-0.05 part, biotin 0.01-4 part, vitamin C 0.05-6 part, Choline Chloride 0.1-6 part, bentonite 30-80 part form, and it is dropped in mixer and mixed successively, add feeding;

2) premix is by cobalt chloride 0.01-5 part, copper sulphate 0.2-8 part, KI 0.05-8 part, ferrous sulfate 0.5-15 part, manganese sulfate 1-20 part, sodium selenite 0.05-5 part, zinc sulfate 0.5-20 part, vitamin A 0.01-0.8 part, vitamin D 0.005-0.5 part, vitamin e1-35 part, thiamine 0.05-5 part, riboflavin 0.01-1 part, nicotinic acid 0.01-1 part, pantothenic acid 0.01-1 part, lipoic acid 10-90 part, Cobastab ₆0.001-0.05 part, biotin 0.01-4 part, vitamin C 0.05-6 part, Choline Chloride 0.1-6 part, bentonite 30-80 part form, and by it, drop in mixer and mix successively, add feeding;

Every dry goods concentrate supplement is fed: every day early during 9:00, during late 20:00, twice, each scale of feeding is 0.25-4kg, free choice feeding high quality grass, licks salt and drinking-water afterwards.

Research mechanism of the present invention and effect: the mixed ration formula of screening, suitable here over sixty years of age horse nursing, can meet the demand of required energy, protein and various trace elements of the growth of horse over sixty years of age here and training, improve exercise performance and the oxidation resistance of horse over sixty years of age here, reach former designing requirement and technique effect, shown technological progress.

The specific embodiment

The present invention is further described in conjunction with the embodiments.

Embodiment

Feed ingredient 1

Get 8 parts of corns, 25 parts, wheat bran, 25 parts, inferior powder, 30 parts of Soybean Meaies, 8 parts of calcium monohydrogen phosphates, 3 parts of salt, 1 part of premix; Its component is placed in to a system machine, makes after the pellet of diameter 4mm Direct-fed;

Wherein premix: get 0.01 part of cobalt chloride, 0.2 part, copper sulphate, 0.05 part of KI, 0.5 part, ferrous sulfate, 1 part of manganese sulfate, 0.05 part of sodium selenite, 0.5 part, zinc sulfate, 0.01 part of vitamin A, 0.005 part of vitamin D, vitamin e1 part, thiamine 0.05-part, 0.01 part, riboflavin, 0.01 part, nicotinic acid, 0.01 part, pantothenic acid, Cobastab ₆0.001 part, 0.01 part of biotin, 0.05 part of vitamin C, 0.1 part of Choline Chloride, 40 parts of bentonites; Its component is dropped in mixer and mixed, can add and feed.

Feed ingredient 2

Get 83 parts of corns, 2.5 parts, wheat bran, 0.5 part, inferior powder, 10 parts of Soybean Meaies, 0.5 part of calcium monohydrogen phosphate, 3 parts of salt, 0.5 part of premix; Its component is placed in to a system machine, makes after the pellet of diameter 3.9mm Direct-fed;

Wherein premix: get 5 parts of cobalt chlorides, 8 parts, copper sulphate, 8 parts of KIs, 15 parts, ferrous sulfate, 20 parts of manganese sulfates, 5 parts of sodium selenites, 20 parts, zinc sulfate, 0.8 part of vitamin A, 0.5 part of vitamin D, 5 parts of vitamin E2s, 5 parts of thiamines, 1 part, riboflavin, 1 part, nicotinic acid, 1 part, pantothenic acid, Cobastab ₆0.05 part, 4 parts of biotins, 6 parts of vitamin Cs, 6 parts of Choline Chlorides, 32 parts of bentonites; Said components is dropped in mixer and mixed, can add and feed.

Feed ingredient 3

Get 35 parts of corns, 15 parts, wheat bran, 10 parts, inferior powder, 20 parts of Soybean Meaies, 6 parts of calcium monohydrogen phosphates, 8 parts of salt, 6 parts of premixes; Its component is placed in to a system machine, makes after the pellet of diameter 4.1mm Direct-fed;

Wherein premix: get 0.17 part of cobalt chloride, 1.65 parts, copper sulphate, 0.4 part of KI, 5.5 parts, ferrous sulfate, 5.24 parts of manganese sulfates, 1 part of sodium selenite, 4.8 parts, zinc sulfate, 0.15 part of vitamin A, 0.02 part of vitamin D, 5.3 parts of vitamin Es, 0.2 part of thiamine, 0.1 part, riboflavin, 0.1 part, nicotinic acid, 0.05 part, pantothenic acid, 38 parts of lipoic acids, Cobastab ₆0.01 part, 0.1 part of biotin, 0.2 part of vitamin C, 1.2 parts of Choline Chlorides, 73 parts of bentonites; Its component is dropped in mixer and mixed, can add and feed.

Feed ingredient 4

Get 45 parts of corns, 25 parts, wheat bran, 15 parts, inferior powder, 10 parts of Soybean Meaies, 2.2 parts of calcium monohydrogen phosphates, 1.8 parts of salt, 1 part of composition of premix; Its component is placed in to a system machine, makes after the pellet of diameter 4mm Direct-fed;

Wherein premix: get 2.5 parts of cobalt chlorides, 4 parts, copper sulphate, 4 parts of KIs, 7.5 parts, ferrous sulfate, 10 parts of manganese sulfates, 2.5 parts of sodium selenites, 10 parts, zinc sulfate, 0.4 part of vitamin A, 0.25 part of vitamin D, 7.5 parts of vitamin Es, 2.5 parts of thiamines, 0.5 part, riboflavin, 0.5 part, nicotinic acid, 0.5 part, pantothenic acid, 80 parts of lipoic acids, Cobastab ₆0.025 part, 2 parts of biotins, 3 parts of vitamin Cs, 3 parts of Choline Chlorides, 55 parts of bentonites; Said components is dropped in mixer and mixed, can add and feed.

Experiment effect checking:

1 materials and methods

1.1 material

Choosing sports achievement, body weight, body chi, agematched here over sixty years of age horse is experimental animal.

1.2 experimental design and feeding and management

By 19, here over sixty years of age, horse was divided into 5 groups at random, wherein 3 test horses of control group; Test group is divided into: 4 test horses of test I group, 4 test horses of test II group, 4 test horses of test III group, 4 test horses of test IV group, each test group component 1,2,3,4 concentrate supplements of feeding respectively, all test horses, except Diet Formula difference, all the other feedings and managements are identical.

Every dry goods concentrate supplement is fed: every day early during 9:00, during late 20:00, twice, each scale of feeding is 0.25-4kg, free choice feeding high quality grass, licks salt and drinking-water afterwards.

Test horses are planned according to stud-farm is conventional:

In regulation game, within first 3 months, train, regulation game first trimester every other day carries out 8km training (except the rainy day), regulation game the first two months every other day carries out 15km training (except the rainy day), every three days, carry out the training of 28km the first half of the month of the previous moon of regulation game, the second half of the month, is every other day carried out the training of 7km, more than training is all carried out after the 2.5h that feeds, and in training every day, finishes just can drink water after 1h.

1.3 simulated race schemes

19 endurance horses to test carry out 20km simulated race, warm-up to each group test horses 2km that jogs before match, before simulated race, after 30min, match, in 30min, measure the temperature pulse respiration of each group test horses, record the contest result of each horses, after match 30min, take a blood sample.

1.4 testing index

1.4.1 exercise performance and physical signs

Average speed, heart rate, body temperature, respiratory rate

1.4.2 blood plasma Antioxidant Indexes

Catalase (CAT), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-PX), antioxidative activities (T-AOC), MDA (MDA).

1.5 data analysis

Result of the test represents with average (Mean) ± standard deviation (SD); Test data all adopts SPSS18.0 software to carry out variance analysis; Each is organized result and adopts Duncan method to carry out multiple ratio.

2 results and analysis

2.1 supplementary feedings and add the impact of antioxidant horse simulated race over sixty years of age post-games exercise performance on here, in Table 1.

Table 1 supplementary feeding and add the impact of antioxidant horse simulation over sixty years of age post-games exercise performance on here

Note: the different lowercase alphabets of colleague's data shoulder mark show significant difference (P < 0.05), the different capitalizations of shoulder mark represent difference extremely significantly (P < 0.01), take on mark same letter or represent difference not remarkable (P > 0.05) without letter mark, lower same.

From table 2 data, test I group, test II group here over sixty years of age horse average speed compare equal difference not significantly (P>0.05) with control group, but test group is compared average speed and is all had rising trend with control group; Behind test I group, test II group Yan Qi Marseille 0min heart rate respectively lower by 20.29% than control group, 23.57%, all reached the utmost point level of signifiance (P < 0.01), when post-games 20min, test I group, test II group heart rate are all extremely significantly lower than control group (P < 0.01), and difference not significantly (P>0.05) between, 53.62%, two test group lower by 43.89% than control group respectively; After simulated race, body temperature difference all not significantly (P>0.05) between test I group, test II group and control group; Here horse test over sixty years of age II group calling suction frequency ratio control group is low by 32.42% for post-games 5min, difference is (P < 0.01) extremely significantly, test I group respiratory rate is compared significant difference (P < 0.05) with control group, and than difference between low 17.16%, two test group of control group not significantly (P>0.05).

Test III group, test IV group here over sixty years of age horse average speed compare equal difference not significantly (P>0.05) with control group, but test group is compared average speed and is had rising trend with control group; The heart rate that behind test III group, test IV group Yan Qi Marseille, 0min and post-games 20min record is all extremely significantly lower than control group (P < 0.01), difference not remarkable (P > 0.05) between two test group; After simulated race, the body temperature that test III group, test IV group record is compared equal difference not remarkable (P > 0.05) with control group; Behind test III group, test IV group Yan Qi Marseille 5min respiratory rate respectively lower by 13.21% than control group, 12.34%, all reached the utmost point level of signifiance (P < 0.01), difference not significantly (P > 0.05) between two test group.

In feed, add antioxidant; test group compared with control group Yan Qi Marseille after physiological function recover faster; illustrate that antioxidant removed in time body endogenous movement and produced too much oxygen radical; reduced the attack of oxygen radical to myocardial cell membrane; protected cardiac muscle cell's normal configuration and function, test group add antioxidant for Yan Qi Marseille after the recovery of exercise performance there is important effect.

2.2 supplementary feedings and add the impact of antioxidant horse simulated race over sixty years of age post-games plasma antioxidant capacity on here, in Table 2.

Table 2 supplementary feeding and add the impact of antioxidant horse simulation over sixty years of age post-games plasma antioxidant capacity on here

From upper table 2 data, compare with control group, the equal difference of CAT vigor not significantly (P > 0.05) in test I group, test II group endurance horse blood plasma, but test I group is compared the trend slightly raising with control group; In test I group, test II group endurance horse blood plasma GSH-PX vigor respectively higher by 60.06% than control group, 75.16%, all reached the utmost point level of signifiance (P < 0.01), difference not significantly (P > 0.05) between two test group; Test I group, test II group here in horse blood plasma over sixty years of age SOD vigor be all significantly higher than control group (P < 0.05), and difference not significantly (P > 0.05) between, 38.25%, two test group higher by 31.27% than control group respectively; Equal difference not significantly (P > 0.05) between two test group T-AOC and control group, but test II group T-AOC is a little more than control group; Test I group, test II group MDA concentration are all extremely significantly lower than control group (P < 0.01).

By table 2, drawn, in test III group endurance horse blood plasma CAT vigor respectively higher by 44.56% than control group, test IV group, 46.62%, reach the level of signifiance (P < 0.05), test IV group and control group difference is remarkable (P > 0.05) not; In test III group endurance horse blood plasma, GSH-PX vigor is higher by 44.30% than control group, reached the level of signifiance (P < 0.05), test IV group is a little more than control group (P > 0.05), and test III group and test IV group difference is remarkable (P > 0.05) not; In test III group and test IV group endurance horse blood plasma SOD vigor respectively higher by 42.33% than control group, 44.72%, inequality heteropole is (P < 0.01) significantly, difference not significantly (P > 0.05) between two test group; In test III group and test IV group endurance horse blood plasma T-AOC size respectively higher by 77.41% than control group, 68.62%, but do not reach the level of signifiance (P > 0.05); In test III group endurance horse blood plasma, MDA concentration ratio control group is low by 69.07%, significant difference (P < 0.05), test IV group is lower by 30.16% than control group, difference is remarkable (P > 0.05) not, test III group and test IV group difference is remarkable (P > 0.05) not, wherein tests III group a little less than test IV group.

In feed, add antioxidant, improve blood plasma GSH-PX, SOD after here horse motion over sixty years of age, T-AOC vigor, reduce MDA concentration, prompting supplementary feeding and add the anti-motion oxidative stress ability that antioxidant can improve horse over sixty years of age here.

The present invention tests explanation, for motion Malaysia, say, heart rate is an important indicator of reflection competition term exercise performance, the medium-term and long-term high heart rate of enduro, cause metabolism crisis, drained, also be the main cause that speed ability reduces simultaneously, low heart rate is a standard of weighing the health degree of horses, in order to evaluate horse, whether be applicable to arduous enduro, in International Equestrian Federation's enduro rule, stipulate, field is after each stage finishes, enter in veterinary inspection point 20min, heart rate should drop to 64beats/min following (containing 64beats/min), if surpassing these horses eliminates.When body is upset, during as strenuous exercise, cause activity in vivo oxygen and free radical to pile up, catalase (CAT) is extensively distributed in cell, and its main biochemical function is Catalytic Oxygen free radical--hydrogen peroxide (H ₂o ₂) resolve into water (H ₂o) and oxygen (O ₂), the non-oxidizability of enhancing body, glutathione peroxidase (GSH-PX) is in body, to remove the most important enzyme of free radical, the Tissue distribution of GSH-px is more extensive than catalase, the electronic catalytic hydrogen peroxide (H that GPX can utilize reduced glutathione, sulphur hydrogen reduction protein, paddy hydrogen reduction protein to provide ₂o ₂), hydroperoxides (ROOH), alcohols (ROH) generate water, remove in time body because of the free radical that strenuous exercise produces, and reduce the damage to body, improve the antioxygenic property of body, superoxide dismutase (SOD) impels O ₂ ^-to H ₂o ₂transform, its activity size can reflect the generation situation of active oxygen to a certain extent, the size of TAC (T-AOC) is subject to the common adjusting of enzyme system antioxidant and non-enzyme system antioxidant, in physiological range, the raising of TAC, effectively protective tissue organ is avoided oxidative stress damage, MDA (MDA) is the little molecular product that peroxidatic reaction of lipid chain type termination phase produces, the level of lipid peroxidation that can indirectly reflect production and the body tissue cell of free radical, oxidative stress or the fatigue state of the rising indication body of its concentration strengthen or aggravate.

The cobalt chloride that this method is selected, KI, ferrous sulfate, sodium selenite, thiamine, nicotinic acid, riboflavin, Choline Chloride, manganese sulfate, lipoic acid are purchased from Chengdu Shu Xing feed corporation,Ltd; Biotin is purchased from Zhejiang NHU Company Ltd; Bentonite is purchased from Hengyang City Shi Cheng feed Co., Ltd; The system of selecting a machine model is MUZL350, and manufacturer is Jiangsu shepherd device fabrication company; The model of mixer is 0.5TSSHJ1, and manufacturer is Jiangsu Zheng Chang grain machine equipment Co., Ltd.

Claims (2)

1. a concentrate supplement that improves endurance horse exercise performance and oxidation resistance, is characterized in that: raw material is prepared with parts by weight;

The preparation of concentrate supplement: raw material components is corn 8-83 part, wheat bran 0.5-55 part, inferior powder 0.5-40.5 part, Soybean Meal 1-40 part, calcium monohydrogen phosphate 0.4-15 part, salt 0.1-20 part, premix 0.1-16 part; Its raw material is placed in to a system machine successively, makes the particle that diameter is 3.5-4.5mm;

Wherein premix is obtained by following two kinds of formulas:

1) premix is by cobalt chloride 0.01-5 part, copper sulphate 0.2-8 part, KI 0.05-8 part, ferrous sulfate 0.5-15 part, manganese sulfate 1-20 part, sodium selenite 0.05-5 part, zinc sulfate 0.5-20 part, vitamin A 0.01-0.8 part, vitamin D 0.005-0.5 part, vitamin E 1-35 part, thiamine 0.05-5 part, riboflavin 0.01-1 part, nicotinic acid 0.01-1 part, pantothenic acid 0.01-1 part, Cobastab ₆0.001-0.05 part, biotin 0.01-4 part, vitamin C 0.05-6 part, Choline Chloride 0.1-6 part, bentonite 30-80 part form, and it is dropped in mixer and mixed successively, add feeding;

2) premix is by cobalt chloride 0.01-5 part, copper sulphate 0.2-8 part, KI 0.05-8 part, ferrous sulfate 0.5-15 part, manganese sulfate 1-20 part, sodium selenite 0.05-5 part, zinc sulfate 0.5-20 part, vitamin A 0.01-0.8 part, vitamin D 0.005-0.5 part, vitamin E 1-35 part, thiamine 0.05-5 part, riboflavin 0.01-1 part, nicotinic acid 0.01-1 part, pantothenic acid 0.01-1 part, lipoic acid 10-90 part, Cobastab ₆0.001-0.05 part, biotin 0.01-4 part, vitamin C 0.05-6 part, Choline Chloride 0.1-6 part, bentonite 30-80 part form, and by it, drop in mixer and mix successively, add feeding;

Every dry goods concentrate supplement is fed: every day early during 9:00, during late 20:00, twice, each scale of feeding is 0.25-4kg, free choice feeding high quality grass, licks salt and drinking-water afterwards.

2. according to concentrate supplement described in claim 1, it is characterized in that: the raw material of selecting is commercially available prod.