CN103987834B - 白细胞分析系统和方法 - Google Patents

白细胞分析系统和方法 Download PDF

Info

Publication number
CN103987834B
CN103987834B CN201280023386.7A CN201280023386A CN103987834B CN 103987834 B CN103987834 B CN 103987834B CN 201280023386 A CN201280023386 A CN 201280023386A CN 103987834 B CN103987834 B CN 103987834B
Authority
CN
China
Prior art keywords
wbc
blood sample
sample
fluorescence
fluorescent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201280023386.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN103987834A (zh
Inventor
吴炯
贾科莫·瓦卡
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott GmbH and Co KG
Original Assignee
Abbott GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott GmbH and Co KG filed Critical Abbott GmbH and Co KG
Publication of CN103987834A publication Critical patent/CN103987834A/zh
Application granted granted Critical
Publication of CN103987834B publication Critical patent/CN103987834B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/88—Investigating the presence of flaws or contamination
    • G01N21/91—Investigating the presence of flaws or contamination using penetration of dyes, e.g. fluorescent ink
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1488—Methods for deciding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1738—Optionally different kinds of measurements; Method being valid for different kinds of measurement
    • G01N2021/174—Optionally different kinds of measurements; Method being valid for different kinds of measurement either absorption-reflection or emission-fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Ecology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201280023386.7A 2011-05-04 2012-04-26 白细胞分析系统和方法 Expired - Fee Related CN103987834B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161482541P 2011-05-04 2011-05-04
US61/482,541 2011-05-04
PCT/US2012/035158 WO2012151102A2 (en) 2011-05-04 2012-04-26 White blood cell analysis system and method

Publications (2)

Publication Number Publication Date
CN103987834A CN103987834A (zh) 2014-08-13
CN103987834B true CN103987834B (zh) 2017-03-29

Family

ID=47090459

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280023386.7A Expired - Fee Related CN103987834B (zh) 2011-05-04 2012-04-26 白细胞分析系统和方法

Country Status (6)

Country Link
US (4) US9091624B2 (enExample)
EP (1) EP2705134B1 (enExample)
JP (1) JP6104233B2 (enExample)
CN (1) CN103987834B (enExample)
ES (1) ES2927316T3 (enExample)
WO (1) WO2012151102A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8986944B2 (en) 2001-10-11 2015-03-24 Aviva Biosciences Corporation Methods and compositions for separating rare cells from fluid samples
US8980568B2 (en) 2001-10-11 2015-03-17 Aviva Biosciences Corporation Methods and compositions for detecting non-hematopoietic cells from a blood sample
EP2041299A4 (en) 2006-07-14 2010-01-13 Aviva Biosciences Corp METHOD AND COMPOSITIONS FOR DETECTION OF RARE CELLS FROM A BIOLOGICAL SAMPLE
WO2013101603A1 (en) * 2011-12-27 2013-07-04 Abbott Laboratories Cellular analysis of body fluids
CN104981692B (zh) * 2013-03-12 2019-09-20 雅培实验室 用于分析白细胞的试剂、体系及方法
EP2997363A4 (en) * 2013-05-13 2016-11-30 Chiranjit Deka APPARATUS AND METHODS FOR CELL ANALYSIS
US10215683B2 (en) 2015-11-02 2019-02-26 Chiranjit Deka Light scatter based apparatus and methods for hematology analysis using only three detectors
WO2020037305A1 (en) * 2018-08-16 2020-02-20 Essenlix Corporation Cell analysis in body fluids, particularly blood
CN112424582B (zh) * 2018-08-29 2024-08-02 深圳迈瑞生物医疗电子股份有限公司 血液样本检测的方法、血液样本检测仪和存储介质
BR102023003038A2 (pt) 2022-03-17 2024-01-02 Sysmex Corporation Método para classificar glóbulos brancos em subpopulações

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5631165A (en) * 1994-08-01 1997-05-20 Abbott Laboratories Method for performing automated hematology and cytometry analysis
US5891734A (en) * 1994-08-01 1999-04-06 Abbott Laboratories Method for performing automated analysis
CN101349644A (zh) * 2007-07-20 2009-01-21 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661913A (en) 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4727020A (en) 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
US5175109A (en) * 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
US4882284A (en) 1987-04-13 1989-11-21 Ortho Pharmaceutical Corporation Method for quantitating and differentiating white blood cells
IE76732B1 (en) * 1990-08-07 1997-11-05 Becton Dickinson Co One step test for absolute counts
JPH07113632B2 (ja) 1991-04-22 1995-12-06 株式会社日立製作所 白血球分析方法
US5776709A (en) 1991-08-28 1998-07-07 Becton Dickinson And Company Method for preparation and analysis of leukocytes in whole blood
WO1993005478A1 (en) 1991-08-28 1993-03-18 Becton, Dickinson & Company Gravitational attractor engine for adaptively autoclustering n-dimensional data streams
DE69329353T2 (de) * 1992-03-05 2001-01-04 Becton Dickinson And Co., Franklin Lakes Verfahren zur Herstellung und Analyse von Leukozyten in Vollblut unter Verwendung der Durchflusszytometrie
US5656499A (en) * 1994-08-01 1997-08-12 Abbott Laboratories Method for performing automated hematology and cytometry analysis
ATE252938T1 (de) * 1994-08-01 2003-11-15 Abbott Lab Verfahren und vorrichtung zur vorbereitung einer flüssigkeit
US5812419A (en) * 1994-08-01 1998-09-22 Abbott Laboratories Fully automated analysis method with optical system for blood cell analyzer
US5559037A (en) 1994-12-15 1996-09-24 Abbott Laboratories Method for rapid and simultaneous analysis of nucleated red blood cells
US5879900A (en) * 1994-12-15 1999-03-09 Abbott Laboratories Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differentials
DE69638209D1 (de) * 1995-12-15 2010-08-12 Abbott Lab Verfahren für gleichzeitige analyse von zelllebensfähigkeit, nukleierten roten blutkörperchen und leukozytendifferential
US6197593B1 (en) * 1998-10-20 2001-03-06 Coulter International Corp. Method for enumerating blood cells
US6228652B1 (en) * 1999-02-16 2001-05-08 Coulter International Corp. Method and apparatus for analyzing cells in a whole blood sample
US6900023B1 (en) 1999-09-02 2005-05-31 Sysmex Corporation Method for classifying and counting leukocytes
US6368864B1 (en) 2000-05-05 2002-04-09 Coulter International Corp. Dyes and methods of reticulocyte enumeration
FR2821428B1 (fr) 2001-02-23 2004-08-06 Abx Sa Reactif et procede pour l'identification et le comptage de cellules biologiques
EP1442141A4 (en) 2001-10-17 2005-05-18 Univ Utah Res Found METHOD FOR IDENTIFYING DIFFERENTIALLY EXPRESSED GENES THROUGH MULTIVARIATE ANALYSIS OF MICROARRAY DATA
JP2003310299A (ja) 2002-04-25 2003-11-05 Sysmex Corp 乳試料中に含まれる体細胞を測定するための乳試料処理方法及び試薬ならびに方法
US7625712B2 (en) 2004-05-21 2009-12-01 Beckman Coulter, Inc. Method for a fully automated monoclonal antibody-based extended differential
US7674598B2 (en) 2004-05-21 2010-03-09 Beckman Coulter, Inc. Method for a fully automated monoclonal antibody-based extended differential
US7299135B2 (en) 2005-11-10 2007-11-20 Idexx Laboratories, Inc. Methods for identifying discrete populations (e.g., clusters) of data within a flow cytometer multi-dimensional data set
WO2007085266A1 (en) 2006-01-30 2007-08-02 Dako Denmark A/S High-speed quantification of antigen specific t-cells in whole blood by flow cytometry
JP4796443B2 (ja) 2006-06-08 2011-10-19 シスメックス株式会社 試料分析用試薬、試料分析用試薬キット及び試料分析方法
JP4914656B2 (ja) 2006-06-26 2012-04-11 シスメックス株式会社 試料分析用試薬、試料分析用試薬キット及び試料分析方法
US7674622B2 (en) * 2006-12-22 2010-03-09 Abbott Laboratories, Inc. Method for determination of nucleated red blood cells and leukocytes in a whole blood sample in an automated hematology analyzer
EP2166353B1 (en) * 2007-06-25 2018-08-08 Sysmex Corporation Reagent and reagent kit for analysis of primitive leukocyte
US8148101B2 (en) 2008-07-22 2012-04-03 Abbott Laboratories Method for classifying and counting bacteria in body fluids
JP5871792B2 (ja) * 2009-04-27 2016-03-01 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ 自動血液分析器において、レーザーからの前方散乱を用いることによる、赤血球細胞を白血球細胞から判別する方法
US8911669B2 (en) * 2009-08-24 2014-12-16 Abbott Laboratories Method for flagging a sample
JP5530691B2 (ja) * 2009-09-25 2014-06-25 シスメックス株式会社 血球計数装置、診断支援装置、診断支援方法及びコンピュータプログラム

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5631165A (en) * 1994-08-01 1997-05-20 Abbott Laboratories Method for performing automated hematology and cytometry analysis
US5891734A (en) * 1994-08-01 1999-04-06 Abbott Laboratories Method for performing automated analysis
CN101349644A (zh) * 2007-07-20 2009-01-21 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法
US20090023129A1 (en) * 2007-07-20 2009-01-22 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. White blood cell differentiation reagent and method of use thereof

Also Published As

Publication number Publication date
US20200141857A1 (en) 2020-05-07
JP2014520251A (ja) 2014-08-21
US20160018310A1 (en) 2016-01-21
WO2012151102A3 (en) 2014-05-08
EP2705134A4 (en) 2015-06-17
EP2705134A2 (en) 2014-03-12
US10481071B2 (en) 2019-11-19
JP6104233B2 (ja) 2017-03-29
US9778162B2 (en) 2017-10-03
ES2927316T3 (es) 2022-11-04
EP2705134B1 (en) 2022-08-24
WO2012151102A2 (en) 2012-11-08
CN103987834A (zh) 2014-08-13
US9091624B2 (en) 2015-07-28
US20180128731A1 (en) 2018-05-10
US20120282598A1 (en) 2012-11-08

Similar Documents

Publication Publication Date Title
CN103987834B (zh) 白细胞分析系统和方法
CN103975054B (zh) 有核红细胞分析系统和方法
US10481073B2 (en) Basophil analysis system and method
JP6987110B2 (ja) 白血球を分析するための試薬、システム、及び方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170329

CF01 Termination of patent right due to non-payment of annual fee