ES2927316T3 - Sistema y método de análisis de glóbulos blancos - Google Patents
Sistema y método de análisis de glóbulos blancos Download PDFInfo
- Publication number
- ES2927316T3 ES2927316T3 ES12779974T ES12779974T ES2927316T3 ES 2927316 T3 ES2927316 T3 ES 2927316T3 ES 12779974 T ES12779974 T ES 12779974T ES 12779974 T ES12779974 T ES 12779974T ES 2927316 T3 ES2927316 T3 ES 2927316T3
- Authority
- ES
- Spain
- Prior art keywords
- wbc
- fluorescence
- signals
- analysis
- blood cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
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- 238000000034 method Methods 0.000 title claims abstract description 62
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- 239000008280 blood Substances 0.000 claims abstract description 57
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 35
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- 238000004820 blood count Methods 0.000 description 3
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- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
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- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
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- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/88—Investigating the presence of flaws or contamination
- G01N21/91—Investigating the presence of flaws or contamination using penetration of dyes, e.g. fluorescent ink
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/016—White blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1477—Multiparameters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1488—Methods for deciding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N2021/1738—Optionally different kinds of measurements; Method being valid for different kinds of measurement
- G01N2021/174—Optionally different kinds of measurements; Method being valid for different kinds of measurement either absorption-reflection or emission-fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Ecology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161482541P | 2011-05-04 | 2011-05-04 | |
| PCT/US2012/035158 WO2012151102A2 (en) | 2011-05-04 | 2012-04-26 | White blood cell analysis system and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2927316T3 true ES2927316T3 (es) | 2022-11-04 |
Family
ID=47090459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12779974T Active ES2927316T3 (es) | 2011-05-04 | 2012-04-26 | Sistema y método de análisis de glóbulos blancos |
Country Status (6)
| Country | Link |
|---|---|
| US (4) | US9091624B2 (enExample) |
| EP (1) | EP2705134B1 (enExample) |
| JP (1) | JP6104233B2 (enExample) |
| CN (1) | CN103987834B (enExample) |
| ES (1) | ES2927316T3 (enExample) |
| WO (1) | WO2012151102A2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8986944B2 (en) | 2001-10-11 | 2015-03-24 | Aviva Biosciences Corporation | Methods and compositions for separating rare cells from fluid samples |
| US8980568B2 (en) | 2001-10-11 | 2015-03-17 | Aviva Biosciences Corporation | Methods and compositions for detecting non-hematopoietic cells from a blood sample |
| EP2041299A4 (en) | 2006-07-14 | 2010-01-13 | Aviva Biosciences Corp | METHOD AND COMPOSITIONS FOR DETECTION OF RARE CELLS FROM A BIOLOGICAL SAMPLE |
| WO2013101603A1 (en) * | 2011-12-27 | 2013-07-04 | Abbott Laboratories | Cellular analysis of body fluids |
| CN104981692B (zh) * | 2013-03-12 | 2019-09-20 | 雅培实验室 | 用于分析白细胞的试剂、体系及方法 |
| EP2997363A4 (en) * | 2013-05-13 | 2016-11-30 | Chiranjit Deka | APPARATUS AND METHODS FOR CELL ANALYSIS |
| US10215683B2 (en) | 2015-11-02 | 2019-02-26 | Chiranjit Deka | Light scatter based apparatus and methods for hematology analysis using only three detectors |
| WO2020037305A1 (en) * | 2018-08-16 | 2020-02-20 | Essenlix Corporation | Cell analysis in body fluids, particularly blood |
| CN112424582B (zh) * | 2018-08-29 | 2024-08-02 | 深圳迈瑞生物医疗电子股份有限公司 | 血液样本检测的方法、血液样本检测仪和存储介质 |
| BR102023003038A2 (pt) | 2022-03-17 | 2024-01-02 | Sysmex Corporation | Método para classificar glóbulos brancos em subpopulações |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4661913A (en) | 1984-09-11 | 1987-04-28 | Becton, Dickinson And Company | Apparatus and method for the detection and classification of articles using flow cytometry techniques |
| US4727020A (en) | 1985-02-25 | 1988-02-23 | Becton, Dickinson And Company | Method for analysis of subpopulations of blood cells |
| US5175109A (en) * | 1986-09-10 | 1992-12-29 | Toa Medical Electronics Co., Ltd. | Reagent for classifying leukocytes by flow cytometry |
| US4882284A (en) | 1987-04-13 | 1989-11-21 | Ortho Pharmaceutical Corporation | Method for quantitating and differentiating white blood cells |
| IE76732B1 (en) * | 1990-08-07 | 1997-11-05 | Becton Dickinson Co | One step test for absolute counts |
| JPH07113632B2 (ja) | 1991-04-22 | 1995-12-06 | 株式会社日立製作所 | 白血球分析方法 |
| US5776709A (en) | 1991-08-28 | 1998-07-07 | Becton Dickinson And Company | Method for preparation and analysis of leukocytes in whole blood |
| WO1993005478A1 (en) | 1991-08-28 | 1993-03-18 | Becton, Dickinson & Company | Gravitational attractor engine for adaptively autoclustering n-dimensional data streams |
| DE69329353T2 (de) * | 1992-03-05 | 2001-01-04 | Becton Dickinson And Co., Franklin Lakes | Verfahren zur Herstellung und Analyse von Leukozyten in Vollblut unter Verwendung der Durchflusszytometrie |
| US5891734A (en) * | 1994-08-01 | 1999-04-06 | Abbott Laboratories | Method for performing automated analysis |
| US5656499A (en) * | 1994-08-01 | 1997-08-12 | Abbott Laboratories | Method for performing automated hematology and cytometry analysis |
| ATE252938T1 (de) * | 1994-08-01 | 2003-11-15 | Abbott Lab | Verfahren und vorrichtung zur vorbereitung einer flüssigkeit |
| US5812419A (en) * | 1994-08-01 | 1998-09-22 | Abbott Laboratories | Fully automated analysis method with optical system for blood cell analyzer |
| US5631165A (en) * | 1994-08-01 | 1997-05-20 | Abbott Laboratories | Method for performing automated hematology and cytometry analysis |
| US5559037A (en) | 1994-12-15 | 1996-09-24 | Abbott Laboratories | Method for rapid and simultaneous analysis of nucleated red blood cells |
| US5879900A (en) * | 1994-12-15 | 1999-03-09 | Abbott Laboratories | Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differentials |
| DE69638209D1 (de) * | 1995-12-15 | 2010-08-12 | Abbott Lab | Verfahren für gleichzeitige analyse von zelllebensfähigkeit, nukleierten roten blutkörperchen und leukozytendifferential |
| US6197593B1 (en) * | 1998-10-20 | 2001-03-06 | Coulter International Corp. | Method for enumerating blood cells |
| US6228652B1 (en) * | 1999-02-16 | 2001-05-08 | Coulter International Corp. | Method and apparatus for analyzing cells in a whole blood sample |
| US6900023B1 (en) | 1999-09-02 | 2005-05-31 | Sysmex Corporation | Method for classifying and counting leukocytes |
| US6368864B1 (en) | 2000-05-05 | 2002-04-09 | Coulter International Corp. | Dyes and methods of reticulocyte enumeration |
| FR2821428B1 (fr) | 2001-02-23 | 2004-08-06 | Abx Sa | Reactif et procede pour l'identification et le comptage de cellules biologiques |
| EP1442141A4 (en) | 2001-10-17 | 2005-05-18 | Univ Utah Res Found | METHOD FOR IDENTIFYING DIFFERENTIALLY EXPRESSED GENES THROUGH MULTIVARIATE ANALYSIS OF MICROARRAY DATA |
| JP2003310299A (ja) | 2002-04-25 | 2003-11-05 | Sysmex Corp | 乳試料中に含まれる体細胞を測定するための乳試料処理方法及び試薬ならびに方法 |
| US7625712B2 (en) | 2004-05-21 | 2009-12-01 | Beckman Coulter, Inc. | Method for a fully automated monoclonal antibody-based extended differential |
| US7674598B2 (en) | 2004-05-21 | 2010-03-09 | Beckman Coulter, Inc. | Method for a fully automated monoclonal antibody-based extended differential |
| US7299135B2 (en) | 2005-11-10 | 2007-11-20 | Idexx Laboratories, Inc. | Methods for identifying discrete populations (e.g., clusters) of data within a flow cytometer multi-dimensional data set |
| WO2007085266A1 (en) | 2006-01-30 | 2007-08-02 | Dako Denmark A/S | High-speed quantification of antigen specific t-cells in whole blood by flow cytometry |
| JP4796443B2 (ja) | 2006-06-08 | 2011-10-19 | シスメックス株式会社 | 試料分析用試薬、試料分析用試薬キット及び試料分析方法 |
| JP4914656B2 (ja) | 2006-06-26 | 2012-04-11 | シスメックス株式会社 | 試料分析用試薬、試料分析用試薬キット及び試料分析方法 |
| US7674622B2 (en) * | 2006-12-22 | 2010-03-09 | Abbott Laboratories, Inc. | Method for determination of nucleated red blood cells and leukocytes in a whole blood sample in an automated hematology analyzer |
| EP2166353B1 (en) * | 2007-06-25 | 2018-08-08 | Sysmex Corporation | Reagent and reagent kit for analysis of primitive leukocyte |
| CN101349644B (zh) * | 2007-07-20 | 2012-06-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种白细胞分类试剂和其使用方法 |
| US8148101B2 (en) | 2008-07-22 | 2012-04-03 | Abbott Laboratories | Method for classifying and counting bacteria in body fluids |
| JP5871792B2 (ja) * | 2009-04-27 | 2016-03-01 | アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ | 自動血液分析器において、レーザーからの前方散乱を用いることによる、赤血球細胞を白血球細胞から判別する方法 |
| US8911669B2 (en) * | 2009-08-24 | 2014-12-16 | Abbott Laboratories | Method for flagging a sample |
| JP5530691B2 (ja) * | 2009-09-25 | 2014-06-25 | シスメックス株式会社 | 血球計数装置、診断支援装置、診断支援方法及びコンピュータプログラム |
-
2012
- 2012-04-26 JP JP2014509318A patent/JP6104233B2/ja not_active Expired - Fee Related
- 2012-04-26 CN CN201280023386.7A patent/CN103987834B/zh not_active Expired - Fee Related
- 2012-04-26 ES ES12779974T patent/ES2927316T3/es active Active
- 2012-04-26 WO PCT/US2012/035158 patent/WO2012151102A2/en not_active Ceased
- 2012-04-26 US US13/456,729 patent/US9091624B2/en not_active Expired - Fee Related
- 2012-04-26 EP EP12779974.0A patent/EP2705134B1/en active Active
-
2015
- 2015-07-24 US US14/808,205 patent/US9778162B2/en not_active Expired - Fee Related
-
2017
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2019
- 2019-10-15 US US16/653,452 patent/US20200141857A1/en not_active Abandoned
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|---|---|
| US20200141857A1 (en) | 2020-05-07 |
| JP2014520251A (ja) | 2014-08-21 |
| US20160018310A1 (en) | 2016-01-21 |
| WO2012151102A3 (en) | 2014-05-08 |
| EP2705134A4 (en) | 2015-06-17 |
| EP2705134A2 (en) | 2014-03-12 |
| US10481071B2 (en) | 2019-11-19 |
| CN103987834B (zh) | 2017-03-29 |
| JP6104233B2 (ja) | 2017-03-29 |
| US9778162B2 (en) | 2017-10-03 |
| EP2705134B1 (en) | 2022-08-24 |
| WO2012151102A2 (en) | 2012-11-08 |
| CN103987834A (zh) | 2014-08-13 |
| US9091624B2 (en) | 2015-07-28 |
| US20180128731A1 (en) | 2018-05-10 |
| US20120282598A1 (en) | 2012-11-08 |
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