CN103960466A - Feed additive taking thermopsis lanceolata as main material and preparation method thereof - Google Patents
Feed additive taking thermopsis lanceolata as main material and preparation method thereof Download PDFInfo
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- CN103960466A CN103960466A CN201310053818.5A CN201310053818A CN103960466A CN 103960466 A CN103960466 A CN 103960466A CN 201310053818 A CN201310053818 A CN 201310053818A CN 103960466 A CN103960466 A CN 103960466A
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- lanceleaf thermopsis
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Abstract
A feed additive taking thermopsis lanceolata as a main material is prepared from the following raw materials in parts by weight: 10-20 parts of an effective composition, 79-89 parts of a carrier and 1 part of a surfactant; and the effective composition comprises 4-8 parts of thermopsis lanceolata extractive and 2-6 parts of aloe extractive and is prepared by mixing thermopsis lanceolata extractive and aloe extractive according to a weight ratio of 4-8:2-6. The application method of the feed additive comprises that the feed additive accounting for 0.5-1% by weight of animal feed is added into the animal feed. The feed additive is abundant in quinolizidine alkaloids which have the pharmacological effects of resisting cancers, resisting heart rate disorder, resisting microbes, resisting ulcers, giving play to leukogenic effect, resisting radiation and the like, and especially the relatively high anticancer activity of the quinolizidine alkaloids causes attention of people. At present, there is no report about Chinese herbal medicine feed additives taking thermopsis lanceolata as the main material.
Description
Technical field
The present invention relates to a kind of animal feed additive, be specifically related to a kind ofly for strengthening the physique of livestock and poultry, that improves immunity take feed addictive that lanceleaf thermopsis herb is primary raw material and preparation method thereof.
Background technology
Along with the raising of China's animal husbandry intensive degree and the development of feed industry, antibiotic, chemical synthetic drug and steroid hormone, as the feed addictives such as somatotropin, pest repellant, hormone, flavor enhancement, modifying agent, pigment agent, anticorrisive agent are also generally used thereupon.Evidence, in feed, add for a long time antibiotic, can make some bacterial strain make a variation into antibody-resistant bacterium, drug-fast bacteria passes to drug-resistance factor again other sensitive bacteria, make its medicine to original sensitivity produce resistance, thereby give by these chemoprophylaxis and some disease of the treatment mankind, bring difficulty.At present, many countries all control and use antibiotic or chemical synthesis class additive in legislation, and Chinese herbal medicine is owing to there is no the drawback of chemical synthetic drug, by people, is considered as the feed addictive of new generation that security performance is good.Chinese herbal medicine is as the advantage of feed addictive: have non-specific antibacterial action, directly bactericidal is antiviral, again nutritious health care with the effect that regulates human body immune function; Lower without hazard residue or hazard residue in animal body; Sex pheromone is difficult for Chinese herbal medicine to produce drug resistance; Natural resources of Chinese medicinal materials is abundant, can gather materials on the spot.Therefore, development Chinese herbal feed additive substitute antibiotics class feed addictive, produces natural, safe, healthy animal food for China's feed industry and animal husbandry, all have major and immediate significance and a historic significance for human health.
Lanceleaf thermopsis herb (Thermopsis lanceolata) formal name used at school lanceolata chrysanthemum, it is pulse family (leguminoseae) Thermopsis (Thermopsis) herbaceos perennial, the quinolizidine that this plant is rich in (Quinolizidine) Alkaloid has many-sided pharmacological actions such as the imbalance of anticancer, anti-heart rate, antimicrobial, antiulcer, liter are white, radioresistance, particularly its stronger active anticancer, has caused people's concern.At present, take the Chinese herbal feed additive that lanceleaf thermopsis herb is main side have not been reported.
Summary of the invention
The object of the invention is to overcome the deficiency existing in conventional art and a kind of physique that can strengthen livestock and poultry providing, that improves immunity take feed addictive that lanceleaf thermopsis herb is major ingredient and preparation method thereof,
Batching of the present invention is as follows:
A kind ofly take the feed addictive that lanceleaf thermopsis herb is major ingredient, proportioning by following weight raw material umber is made: carrier and 1 part of surfactant of the effective ingredient of 10～20 parts and 79～89 parts form, described effective ingredient is that lanceleaf thermopsis herb extract accounts for 4-8 part, aloe extract accounts for 2-6 part, the ratio that is 4-8: 2-6 according to weight ratio by lanceleaf thermopsis herb extract and aloe extract is mixed, and described carrier is a kind of in starch, glucose, dextrin.
The preparation method of described lanceleaf thermopsis herb extract is as follows:
Step 1, select materials: choose without disease and pest, the lanceleaf thermopsis herb seed of full grains or the cauline leaf of robust growth, cross 20 mesh sieves after pulverizing;
Step 2, extraction: with alcohol reflux, extract lanceleaf thermopsis herb, extract three times, total extraction time is 3-4h, merges No. three times extract, and decompression recycling ethanol is to cream;
Step 3, dry: lanceleaf thermopsis herb cream is put in vacuum drying chamber, and 40 ℃ of-50 ℃ of vacuum drying to water content are 0, obtain lanceleaf thermopsis herb extract.
The concentration of ethanol described in step 2 is 70%---80%.
The 6-10 that the addition of ethanol described in step 2 is raw material weight doubly.
The preparation method of described aloe extract is as follows:
Step 1, select materials: select annual more than, without the aloe leaf of disease and pest, robust growth;
Step 2, cleaning: wash away surface dirt or silt;
Step 3, pulverizing: with plant pulverizer, pulverize, cross 20 mesh sieves;
Step 4, dipping extract: add raw material weight 4-6 distilled water doubly, soak twice, and each 24h, three layers of filtered through gauze, filtrate merges standby;
Step 5, concentrate drying: after filtrate is concentrated with vacuum concentration equipment, by spray drying device or vacuum dryer, be dried.
Described surfactant is dodecyl sodium sulfate.
The usage of described feed addictive is for to add in animal feed material according to the ratio that accounts for animal feed gross weight 0.5～1%.
Pharmacological effect and the effect of each composition of this feed addictive are as follows:
Lanceleaf thermopsis herb: have phlegm-dispelling functions, herb preserved material is excited vomiting and respiratory center directly, and stimulate the sensory nerve ending of stomach, reflexive promotion bronchial secretion, with the same use of ipecac, the effect that has eliminating the phlegm and deepen to accelerate to breathe; Secondly, be conducive to the effect of breathing, anagyrine or the similar nicotine of sparteine effect, can reflexive excited breathing.There is strong excited respiration simultaneously, while palpitating speed, blood pressure sharply rises, and recovers very soon thereafter normal in company with the disappearance of respiratory stimulant effect.Experiment showed, the excited respiration of 0.06 mg/kg of the sparteine that extracts from this product, be not only greatly better than the antabac of same dosage, and slightly strong compared with the antabac of 5 times (0.3 mgs/kg).Methylcytisine effect is similar to sparteine, but compared with it weak 10～12 times; Three, thermopsine has the inhibitory action of medium degree to vegetative ganglia, reduces adrenal cortex and sino-carotid reactivity, improves uterus muscle tension force.Anagyrine also can suppress neuromere impulse conduction, but, and have curariform action mask.Dried plant has the effect (contact poison) of kill insects, and herb preserved material still can anthelmintic, and the liquid extract intravenous injection of leaf can make exaggerated respiration accelerate, and blood pressure raises, and heart rate accelerates.
Aloe: regulate the immunity (being resistance) of animal comprehensively, some chronic diseases are recovered without treatment, promote cytothesis, animal tissue injured and sclerosis is got well, stimulate circulation, get rid of vivotoxin.Aloe is called as the treasure-house of vitamin, amino acid and mineral matter, can supplement these required materials of animal in all rheum officinale glycoside cathartics, and the excitant of aloe is the strongest, when rushing down down, with significant stomachache and pelvic congestion, when serious, can cause ephritis.The Main Function position of aloe is at large intestine, to small intestine without promoting creeping effect.
The specific embodiment
Below in conjunction with embodiment, preparation method of the present invention is described further:
Embodiment 1
Take dry lanceleaf thermopsis herb seed 1kg, after pulverizing, extract respectively with the alcohol reflux of 9 times of amounts, 7 times of amounts, 5 times of amounts 70%, extraction time is respectively 2h, 1h, and 0.5h, reclaims ethanol, merges concentrate, and vacuum drying, obtains lanceleaf thermopsis herb extract.
Take aloe cured leaf 1kg, take water as solvent impregnated extraction three times after pulverizing, extraction time is respectively 2h, 1h, and 0.5h, spraying is dry, obtains aloe extract.
Lanceleaf thermopsis herb extract, aloe extract are mixed according to the part by weight of 6: 4, obtain mixed extract.
Mixed extract, starch, dodecyl sodium sulfate are mixed according to the ratio of 11: 90: 1, as feed addictive, use.
Take respectively the additive 10kg by above-mentioned preparation, evenly sneak in 1000kg poultry feed and use.
This feed addictive clinical effectiveness of preparing passes through following verification experimental verification:
1. experimental animal: 1 age in days Luo Man egg cock, purchased from Zheng De bio tech ltd, Zhengzhou.Raising to 12 age in days is chosen 360 plumages, is divided into 4 groups, 90 every group, establishes 3 repetitions.1st～2 groups are added respectively 1%, 0.5% feed addictive in basal diet, the 3rd group be immune control group immune vaccine without feed addictive, the 4th group be blank group not immune vaccine without feed addictive.Experimental period is 42 days.
Experimental animal packet transaction is in Table 1.
Table 1 experimental animal packet transaction
2. test by vaccine and test material: the medium virulence live vaccine of infections chicken cloacal bursa, Binzhou, Shandong Wo Hua bioengineering Co., Ltd product, specification: 1000 plumage/bottles; Lot number: 111008; Bursa of farbricius standard positive serum, purchased from China Veterinary Drugs Supervisory Inst.,
3. testing index: before vaccine immunity (14 ages in days, D0) randomly draw 10 plumages blood samplings from full group, with β-microdetermination ND specific antibody as initially tiring.The 7th (D7), 14 (D14) after first immunisation, 21 (D21), 28 (D28), 35 (D35), 42 (D42) day every group get at random 6 chicken wings venous blood collections, fine jade expansion method detects IBD specific antibody titres in serum.
Each group index of surveying the results are shown in Table 2.
Table 2 is respectively organized the variation (log2) that AGP tires
Note: with the different alphabetical person's significant differences (p < 0.05) of column data subscript."-" represents negative antibody.
The result showing from table 2 can be found out: each feed addictive group more than the serum AGP of most time point antibody titer higher than or be significantly higher than immune control group, show, this feed addictive has the immunization after good enhancing Immune Profile In Chicks infectious bursa of Fabricius vaccine.1% dosage group effect and 0.5% dosage group approach.
Embodiment 2
Take dry lanceleaf thermopsis herb seed 1.5kg, after pulverizing, with 10 times of amounts, 8 times of amounts, 6 times of alcohol reflux extractions that amount concentration is 70%, extraction time is respectively 2h respectively, 1h, and 0.5h, reclaims ethanol, merges concentrate, and vacuum drying, obtains lanceleaf thermopsis herb extract.
Take aloe cured leaf 1.5kg, take water as solvent impregnated extraction three times after pulverizing, extraction time is respectively 2h, 1h, and 0.5h, spraying is dry, obtains aloe extract.
Lanceleaf thermopsis herb extract, aloe extract are mixed according to the part by weight of 6: 4, obtain mixed extract.
Mixed extract, starch, dodecyl sodium sulfate are mixed according to the ratio of 20: 79: 1, as feed addictive, use.
Take the additive 5kg by above-mentioned preparation, evenly sneak in 1000kg animal feeding-stuff and use.
This feed addictive clinical effectiveness of preparing passes through following verification experimental verification:
1. experimental animal: 360 plumage 10 age in days Luo Man egg cocks.Be divided into 4 groups, 90 every group, establish 3 repetitions.1st～2 groups are added respectively 1%, 0.5% feed addictive in basal diet, the 3rd group be immune control group immunity without feed addictive, the 4th group is that blank group is not immune without feed addictive.Experimental period is 42 days.
Experimental animal packet transaction is in Table 3.
Table 3 experimental animal packet transaction
2. test is vaccine with vaccine and test material: newcastle disease IV, Binzhou, Shandong Wo Hua bioengineering Co., Ltd product, specification: 1000 plumage/bottles; Lot number: 120122.Ewcastle disease standard antigen, feminine gender and positive serum, purchased from China Veterinary Drugs Supervisory Inst., the antigen HA 1:256 that tires.
3. testing index: before vaccine immunity (120 ages in days, D0) randomly draw 10 plumages blood samplings from full group, with β-microdetermination ND specific antibody as initially tiring.The 7th (D7), 14 (D14) after first immunisation, 21 (D21), 28 (D28), 35 (D35), 42 (D42) day every group get at random 6 chicken wings venous blood collections, detect serum N D specific antibody titres.
Each group index of surveying the results are shown in Table 4.
Table 4 is respectively organized the variation (log2) that ND-HI tires
Note: with the different alphabetical person's significant differences (p < 0.05) of column data subscript.
The result being shown by table 4 can be found out: each feed addictive group the serum N D of most time point antibody titer many higher than or be significantly higher than immune control group, show, this feed addictive has the immunization of better enhancing ND Vaccine.1% dosage group effect and 0.5% dosage group approach, therefore determine that the using method of this Chinese herbal feed additive is: the ratio according to 0.5～1% is added in poultry fodder.
Content of the present invention is not limited to the embodiment described in description, in the situation that having adopted essentiality content of the present invention, within all improvement that the present invention is made and variation still drop on expressed protection domain.
Claims (7)
1. take the feed addictive that lanceleaf thermopsis herb is major ingredient for one kind, it is characterized in that: the proportioning by following weight raw material umber is made: carrier and 1 part of surfactant of the effective ingredient of 10～20 parts and 79～89 parts form, described effective ingredient is that lanceleaf thermopsis herb extract accounts for 4-8 part, aloe extract accounts for 2-6 part, the ratio that is 4-8: 2-6 according to weight ratio by lanceleaf thermopsis herb extract and aloe extract is mixed, and described carrier is a kind of in starch, glucose, dextrin.
2. the feed addictive that the lanceleaf thermopsis herb of take is according to claim 1 major ingredient, is characterized in that: the preparation method of described lanceleaf thermopsis herb extract is as follows:
Step 1, select materials: choose without disease and pest, the lanceleaf thermopsis herb seed of full grains or the cauline leaf of robust growth, cross 20 mesh sieves after pulverizing;
Step 2, extraction: with alcohol reflux, extract lanceleaf thermopsis herb, extract three times, total extraction time is 3-4h, merges No. three times extract, and decompression recycling ethanol is to cream;
Step 3, dry: lanceleaf thermopsis herb cream is put in vacuum drying chamber, and 40 ℃ of-50 ℃ of vacuum drying to water content are 0, obtain lanceleaf thermopsis herb extract.
3. the feed addictive that the lanceleaf thermopsis herb of take is according to claim 2 major ingredient, is characterized in that: the concentration of ethanol described in step 2 is 70%---80%.
4. the feed addictive that the lanceleaf thermopsis herb of take is according to claim 2 major ingredient, is characterized in that: the 6-10 that the addition of ethanol described in step 2 is raw material weight doubly.
5. the feed addictive that the lanceleaf thermopsis herb of take is according to claim 1 major ingredient, is characterized in that: the preparation method of described aloe extract is as follows:
Step 1, select materials: select annual more than, without the aloe leaf of disease and pest, robust growth;
Step 2, cleaning: wash away surface dirt or silt;
Step 3, pulverizing: with plant pulverizer, pulverize, cross 20 mesh sieves;
Step 4, dipping extract: add raw material weight 4-6 distilled water doubly, soak twice, and each 24h, three layers of filtered through gauze, filtrate merges standby;
Step 5, concentrate drying: after filtrate is concentrated with vacuum concentration equipment, by spray drying device or vacuum dryer, be dried.
6. as claimed in claim 1ly take the feed addictive that lanceleaf thermopsis herb is main side, it is characterized in that: described surfactant is dodecyl sodium sulfate.
7. the feed addictive that is main side according to lanceleaf thermopsis herb described in claim 1, is characterized in that: the usage of described feed addictive is for to add in animal feed material according to the ratio that accounts for animal feed gross weight 0.5～1%.
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| CN201310053818.5A CN103960466B (en) | 2013-02-02 | 2013-02-02 | A kind of take lanceleaf thermopsis herb as feed addictive of major ingredient and preparation method thereof |
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| CN103960466A true CN103960466A (en) | 2014-08-06 |
| CN103960466B CN103960466B (en) | 2016-04-20 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982747A (en) * | 2015-07-30 | 2015-10-21 | 韩震伟 | Feed additive based on Thermopsis lanceolata, and preparation method thereof |
| CN106107072A (en) * | 2016-06-22 | 2016-11-16 | 丁大喜 | A kind of Chinese herbal feed for sheep |
Citations (3)
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| CN85108322A (en) * | 1985-11-11 | 1986-07-02 | 和希格宝音 | Bitter beans comprehensive use |
| CN1473828A (en) * | 2003-06-02 | 2004-02-11 | 平 刘 | Porocess for extracting matrine and sophoridine from sophora alopecuroide |
| CN1759720A (en) * | 2004-10-12 | 2006-04-19 | 龚靖宁 | Feedstuff additive of aloe |
-
2013
- 2013-02-02 CN CN201310053818.5A patent/CN103960466B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85108322A (en) * | 1985-11-11 | 1986-07-02 | 和希格宝音 | Bitter beans comprehensive use |
| CN1473828A (en) * | 2003-06-02 | 2004-02-11 | 平 刘 | Porocess for extracting matrine and sophoridine from sophora alopecuroide |
| CN1759720A (en) * | 2004-10-12 | 2006-04-19 | 龚靖宁 | Feedstuff additive of aloe |
Non-Patent Citations (3)
| Title |
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| 吉林省野生经济植物志编辑委员会编著: "《吉林省野生经济植物志》", 30 September 1961, 吉林人民出版社出版 * |
| 李勇等: "牧马豆生物碱抑菌抗炎作用研究", 《农业科学研究》 * |
| 祁俊生等: "绿色芦荟饲料添加剂的研究", 《饲料与畜牧》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982747A (en) * | 2015-07-30 | 2015-10-21 | 韩震伟 | Feed additive based on Thermopsis lanceolata, and preparation method thereof |
| CN106107072A (en) * | 2016-06-22 | 2016-11-16 | 丁大喜 | A kind of Chinese herbal feed for sheep |
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| CN103960466B (en) | 2016-04-20 |
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