CN103948624B - Strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof - Google Patents
Strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof Download PDFInfo
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- CN103948624B CN103948624B CN201410115216.2A CN201410115216A CN103948624B CN 103948624 B CN103948624 B CN 103948624B CN 201410115216 A CN201410115216 A CN 201410115216A CN 103948624 B CN103948624 B CN 103948624B
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Abstract
The invention discloses strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof, relate to veterinary drug technical field.Ferric citrate is joined in distilled water by the present invention, heats and is sufficiently stirred for dissolving, adds zinc gluconate, sodium selenite and sorbitol, heat and be sufficiently stirred for dissolving；Then its pH value is regulated to 4.5～5.5 with 0.1mol/L sodium hydroxide solution, autoclaving 20 minutes, it is packed as 2mL, 5mL or 10mL, last lamp inspection after membrane filtration, prepares the strong fat agent of yak disease-resistant growth-promoting.The present invention uses the reasonably combined of organic trace element, decreases element kind；The method of the present invention simplifies the strong fat agent producing process of yak disease-resistant growth-promoting；The strong fat agent of the yak disease-resistant growth-promoting of the present invention is obvious to promotion growth and the enhancing immunity effect of yak.
Description
Technical field
The present invention relates to veterinary drug technical field, in particular strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof.
Background technology
Trace element is constituent necessary to animal body, to keeping body health important in inhibiting, if trace element deficiency, to cause that internal enzyme, hormonal activity reduce, metabolism disorder, growth retardation,, even there is various disease in immunity degradation, severe patient can bring out death.
At present, yak cultivation is generally adopted ecosystem grassland stocking model, only is difficult to meet body requirement by herbage and water source, and according to report display, North-West Sichuan pastoral area Trace Elements in Soil zinc, ferrum are lower than healthy marginal value, and selenium is famine especially.Due to the shortage of trace element, make pastoral area, plateau yak Nutrition and Metabolism disease rather multiple, seriously constrain the development of yak aquaculture.Mineral element lacks the poor growth and hypoimmunity that cause and Nutrition and Metabolism disease has all had a strong impact on the economic benefit that yak cultivates.Microelement feed addictive easily causes again spice inequality and causes animal poisoning and environmental pollution.Therefore, a kind of balanced supply, compound recipe disease-resistant growth-promoting shape fat agent safe and effective, easy to use, the various trace elements that has no side effect, can supplement simultaneously are developed yak aquaculture is significant.
Summary of the invention
The technical problem to be solved is for prior art Problems existing in cultivation yak, it is provided that strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof.
Technical scheme is as follows:
The preparation method of the strong fat agent of a kind of yak disease-resistant growth-promoting, its technical scheme is: by 4.0～6.0g ferric citrate (C6H5FeO7·5H2O) join in 400～600mL distilled water, heat and be sufficiently stirred for dissolving, add 4.6～6.9g zinc gluconate (C12H22O14Zn), 0.06～0.09g sodium selenite (Na2SeO3·5H2O) and 120～180g sorbitol, heats and is sufficiently stirred for dissolving；Then its pH value is regulated to 4.5～5.5 with 0.1mol/L sodium hydroxide solution, autoclaving 20 minutes, it is packed as 2mL, 5mL or 10mL, last lamp inspection after membrane filtration.
Described ferric citrate (C6H5FeO7·5H2O) for 5.0g, distilled water is 500mL, zinc gluconate (C12H22O14Zn) for 5.75g, sodium selenite (Na2SeO3·5H2O) for 0.075g, sorbitol is 150g.
The strong fat agent of the yak disease-resistant growth-promoting that described preparation method prepares.
The invention have the benefit that one is the reasonably combined of use organic trace element；Two is that element kind reduces；Three is that production technology simplifies；Four is promote that growth and enhancing immunity effect are obvious.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.
The preparation of the strong fat agent of embodiment 1 yak disease-resistant growth-promoting
A, weigh ferric citrate (C6H5FeO7·5H2O) 5.0g, adds 500ml distilled water, heats and be sufficiently stirred for dissolving.
B, add zinc gluconate (C12H22O14Zn) 5.75g, sodium selenite (Na2SeO3·5H2O) 0.075g, sorbitol 150g, heat and be sufficiently stirred for dissolving.
C, adjust pH value to 4.5～5.5 with sodium hydroxide solution (0.1mol/L).
D, autoclaving 20 minutes.
E, membrane filtration.
F, it is packed as 2mL, 5mL or 10mL, lamp inspection.
Embodiment 2
1, body weight 91.2kg ± 3.4kg soft soil slope over plateau 12 about 24 monthly ages is selected, it is randomly divided into matched group and test group, matched group injection 0.1ml/kg normal saline, yak disease-resistant growth-promoting prepared by test group intramuscular injection 0.1ml/kg embodiment strengthens fat agent, and with mtt assay measure after its injection 15,30,45,60d lymphocytic proliferation rate.
2, after contrasting before injection and injecting, the Gain weight of 60d, carries out weight increment test.
3, result
(1), after the strong fat agent of yak injection yak disease-resistant growth-promoting, record different blood sampling time yak lymphocytic proliferation rate test group by mtt assay and be all remarkably higher than matched group, significant difference.Concrete outcome is in Table 1.
Table 1 lymphocytic proliferation rate (SI value)
(2), after the strong fat agent of yak injection yak disease-resistant growth-promoting, in experimental period, test group is notable with matched group 60d weight differences after injection, and gaining effect is obvious, refers to table 2.
Table 2 weight increment test result
As it can be seen from table 1 lymphocyte transformation proliferation assay SI value is all significantly improved than matched group, after namely injecting the strong fat agent of yak disease-resistant growth-promoting to yak, it is remarkably improved its cellular immune function.Table 2 is it can be seen that matched group and experimental group initial weight zero difference, but along with the increase of age in days, and the weightening finish significant difference of test group and matched group, it is known that, the strong fat agent of yak growth promoting can remarkably promote the growth of yak.
Embodiment 3
1, body weight 90kg ± 3.1kg soft soil slope over plateau 12 is selected, it is randomly divided into matched group and test group, matched group injection 0.2ml/kg normal saline, the strong fat agent of yak disease-resistant growth-promoting of test group intramuscular injection 0.2ml/kg embodiment 1 preparation, and with mtt assay measure after its injection 15,30,45,60d lymphocytic proliferation rate..
2, after contrasting before injection and injecting, the Gain weight of 60d, carries out weight increment test.
3, result
(1), after the strong fat agent of yak injection yak disease-resistant growth-promoting, record different blood sampling time yak lymphocytic proliferation rate test group all pole by mtt assay and be significantly higher than matched group, significant difference.Concrete outcome is in Table 3.
Table 3 lymphocytic proliferation rate (SI value)
(2), after the strong fat agent of yak injection yak disease-resistant growth-promoting, in experimental period, test group and matched group are extremely notable with 95d weight differences in age for age at 85d, refer to table 4.
Table 4 weight increment test result
From table 3 it can be seen that lymphocytic proliferation rate test group is improved significantly than matched group, after namely injecting the strong fat agent of yak disease-resistant growth-promoting to yak, its cellular immune function can be improved, and effect is better than embodiment 2.Table 4 is it can be seen that matched group and the basic zero difference of experimental group initial weight, but along with the increase of age in days, and the daily gain significant difference of test group and matched group, it is known that, the strong fat agent of yak disease-resistant growth-promoting can remarkably promote the growth of yak.
It should be appreciated that for those of ordinary skills, it is possible to improved according to the above description or converted, and all these are improved and convert the protection domain that all should belong to claims of the present invention.
Claims (3)
1. the preparation method of the strong fat agent of yak disease-resistant growth-promoting, it is characterized in that, 4.0～6.0g ferric citrate is joined in 400～600mL distilled water, heat and be sufficiently stirred for dissolving, add 4.6～6.9g zinc gluconate, 0.06～0.09g sodium selenite and 120～180g sorbitol, heat and be sufficiently stirred for dissolving；Then its pH value is regulated to 4.5～5.5 with 0.1mol/L sodium hydroxide solution, autoclaving 20 minutes, it is packed as 2mL, 5mL or 10mL, last lamp inspection after membrane filtration.
2. preparation method according to claim 1, is characterized in that, described ferric citrate is 5.0g, and distilled water is 500mL, and zinc gluconate is 5.75g, and sodium selenite is 0.075g, and sorbitol is 150g.
3. the strong fat agent of yak disease-resistant growth-promoting that preparation method according to claim 1 and 2 prepares.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410115216.2A CN103948624B (en) | 2014-03-24 | 2014-03-24 | Strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof |
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| CN201410115216.2A CN103948624B (en) | 2014-03-24 | 2014-03-24 | Strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof |
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| CN103948624B true CN103948624B (en) | 2016-07-06 |
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| CN101978856B (en) * | 2010-11-13 | 2013-04-17 | 兰州大学 | Complex nutrient additive block special for yaks |
| CN103110615A (en) * | 2013-01-15 | 2013-05-22 | 四川农业大学 | Preparation method of microelement zinc-iron raw injection for pig |
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