CN103948624A - Yak disease-resistance, growth-promoting and fat-growing agent and preparation method thereof - Google Patents
Yak disease-resistance, growth-promoting and fat-growing agent and preparation method thereof Download PDFInfo
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- CN103948624A CN103948624A CN201410115216.2A CN201410115216A CN103948624A CN 103948624 A CN103948624 A CN 103948624A CN 201410115216 A CN201410115216 A CN 201410115216A CN 103948624 A CN103948624 A CN 103948624A
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Abstract
The present invention discloses a yak disease-resistance, growth-promoting and fat-growing agent and a preparation method thereof, and relates to the technical field of veterinary drugs. The preparation method comprises: adding iron citrate to double distilled water, heating, completely stirring, dissolving, adding zinc gluconate, sodium selenite and sorbitol, heating, completely stirring, dissolving, adopting a 0.1 mol/L sodium hydroxide solution to adjust the pH value to 4.5-5.5, carrying out high pressure sterilization for 20 min, filtering with a filtration membrane, sub-packaging into the specifications of 2 mL, 5 mL or 10 mL, and carrying out light inspection to prepare the yak disease-resistance, growth-promoting and fat-growing agent. According to the present invention, the reasonable matching of organic trace elements is adopted so as to reduce the element type; with the method, the yak disease-resistance, growth-promoting and fat-growing agent production process is simplified; and the yak disease-resistance, growth-promoting and fat-growing agent has significant effects of growth promoting and immunity enhancing.
Description
Technical field
The present invention relates to veterinary drug technical field, in particular strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof.
Background technology
Trace element is the necessary constituent of animal body, to keeping body health important in inhibiting, if trace element deficiency, to cause body endoenzyme, hormonal activity to reduce, metabolism disorder, growth retardation,, even there is various diseases in immunity degradation, severe patient can bring out death.
At present, the general ecosystem grassland stocking model that adopts of yak cultivation, only depends on herbage and water source to be difficult to meet body requirement, shows according to report, and pastoral area, northwest, river Trace Elements in Soil zinc, ferrum are lower than healthy marginal value, and selenium is famine especially.Due to the shortage of trace element, make pastoral area, plateau yak Nutrition and Metabolism disease rather multiple, seriously restrict the development of yak aquaculture.Mineral element lacks the poor growth that causes and hypoimmunity and Nutrition and Metabolism disease and has all had a strong impact on the economic benefit of yak cultivation.Microelement feed addictive easily causes again spice uneven and cause the poisoning and environmental pollution of animal.Therefore, develop a kind of balanced supply, compound recipe disease-resistant growth-promoting shape fat agent safe and effective, easy to use, that have no side effect, can supplement simultaneously various trace elements is significant to yak aquaculture.
Summary of the invention
Technical problem to be solved by this invention is the problem existing in cultivation yak for prior art, and strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof is provided.
Technical scheme of the present invention is as follows:
A preparation method for the strong fat agent of yak disease-resistant growth-promoting, its technical scheme is: by 4.0～6.0g ferric citrate (C
6h
5feO
75H
2o) join in 400～600mL distilled water, the abundant stirring and dissolving of heating, then add 4.6～6.9g zinc gluconate (C
12h
22o
14zn), 0.06～0.09g sodium selenite (Na
2seO
35H
2o) and 120～180g sorbitol, the abundant stirring and dissolving of heating; Then regulate its pH value to 4.5～5.5 with 0.1mol/L sodium hydroxide solution, autoclaving 20 minutes, is packed as 2mL, 5mL or 10mL after membrane filtration, last lamp inspection.
Described ferric citrate (C
6h
5feO
75H
2o) be 5.0g, distilled water is 500mL, zinc gluconate (C
12h
22o
14zn) be 5.75g, sodium selenite (Na
2seO
35H
2o) be 0.075g, sorbitol is 150g.
The strong fat agent of yak disease-resistant growth-promoting that described preparation method makes.
Beneficial effect of the present invention is: the one, use the reasonably combined of organic trace element; The 2nd, element kind reduces; The 3rd, production technology is simplified; The 4th, promote growth and enhancing immunity successful.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.
The preparation of the strong fat agent of embodiment 1 yak disease-resistant growth-promoting
A, take ferric citrate (C
6h
5feO
75H
2o) 5.0g, adds 500ml distilled water, the abundant stirring and dissolving of heating.
B, add zinc gluconate (C again
12h
22o
14zn) 5.75g, sodium selenite (Na
2seO
35H
2o) 0.075g, sorbitol 150g, the abundant stirring and dissolving of heating.
C, use sodium hydroxide solution (0.1mol/L) adjust pH to 4.5～5.5.
D, autoclaving 20 minutes.
E, membrane filtration.
F, be packed as 2mL, 5mL or 10mL, lamp inspection.
Embodiment 2
1, select about 24 monthly ages 12 of body weight 91.2kg ± 3.4kg plateau type yaks, be divided at random matched group and test group, matched group injection 0.1ml/kg normal saline, the strong fat agent of yak disease-resistant growth-promoting prepared by test group intramuscular injection 0.1ml/kg embodiment, and with mtt assay measure after its injection 15,30,45,60d lymphocytic proliferation rate.
2, Gain weight front and the rear 60d of injection is injected in contrast, carries out weight increment test.
3, result
(1) after the strong fat agent of yak injection yak disease-resistant growth-promoting, record different blood sampling time yak lymphocytic proliferation rate test group by mtt assay and be all significantly higher than matched group, significant difference.Concrete outcome is in table 1.
Table 1 lymphocytic proliferation rate (SI value)
(2) after the strong fat agent of yak injection yak disease-resistant growth-promoting, test group and matched group 60d body weight significant difference after injection in experimental period, gaining effect is obvious, refers to table 2.
Table 2 weight increment test result
As can be seen from Table 1, lymphocyte transformation increment test SI value is all significantly improved than matched group,, to after the strong fat agent of yak injection yak disease-resistant growth-promoting, can significantly improve its cellular immune function.Table 2 can be found out, matched group and experimental group initial weight zero difference, but along with the increase of age in days, the weightening finish significant difference of test group and matched group, known, the short fat agent of becoming strong of yak can significantly promote the growth of yak.
Embodiment 3
1, select 12 of body weight 90kg ± 3.1kg plateau type yaks, be divided at random matched group and test group, matched group injection 0.2ml/kg normal saline, the strong fat agent of yak disease-resistant growth-promoting prepared by test group intramuscular injection 0.2ml/kg embodiment 1, and with mtt assay measure after its injection 15,30,45,60d lymphocytic proliferation rate.。
2, Gain weight front and the rear 60d of injection is injected in contrast, carries out weight increment test.
3, result
(1) after the strong fat agent of yak injection yak disease-resistant growth-promoting, by mtt assay record different blood sampling time yak lymphocytic proliferation rate test group all the utmost point be significantly higher than matched group, significant difference.Concrete outcome is in table 3.
Table 3 lymphocytic proliferation rate (SI value)
(2) after the strong fat agent of yak injection yak disease-resistant growth-promoting, in experimental period, test group and matched group are extremely remarkable in 85d age and 95d body weight in age difference, refer to table 4.
Table 4 weight increment test result
As can be seen from Table 3, lymphocytic proliferation rate test group is improved significantly than matched group,, to after the strong fat agent of yak injection yak disease-resistant growth-promoting, can improve its cellular immune function, and effect is better than embodiment 2.Table 4 can be found out, matched group and the basic zero difference of experimental group initial weight, but along with the increase of age in days, the daily gain significant difference of test group and matched group, known, the strong fat agent of yak disease-resistant growth-promoting can significantly promote the growth of yak.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (3)
1. the preparation method of the strong fat agent of yak disease-resistant growth-promoting, it is characterized in that, 4.0～6.0g ferric citrate is joined in 400～600mL distilled water, the abundant stirring and dissolving of heating, add again 4.6～6.9g zinc gluconate, 0.06～0.09g sodium selenite and 120～180g sorbitol, the abundant stirring and dissolving of heating; Then regulate its pH value to 4.5～5.5 with 0.1mol/L sodium hydroxide solution, autoclaving 20 minutes, is packed as 2mL, 5mL or 10mL after membrane filtration, last lamp inspection.
2. preparation method according to claim 1, is characterized in that, described ferric citrate is 5.0g, and distilled water is 500mL, and zinc gluconate is 5.75g, and sodium selenite is 0.075g, and sorbitol is 150g.
3. the strong fat agent of yak disease-resistant growth-promoting that preparation method according to claim 1 and 2 makes.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410115216.2A CN103948624B (en) | 2014-03-24 | 2014-03-24 | Strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410115216.2A CN103948624B (en) | 2014-03-24 | 2014-03-24 | Strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN103948624A true CN103948624A (en) | 2014-07-30 |
| CN103948624B CN103948624B (en) | 2016-07-06 |
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|---|---|---|---|
| CN201410115216.2A Active CN103948624B (en) | 2014-03-24 | 2014-03-24 | Strong fat agent of a kind of yak disease-resistant growth-promoting and preparation method thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101978856A (en) * | 2010-11-13 | 2011-02-23 | 兰州大学 | Complex nutrient additive block special for yaks |
| CN103110615A (en) * | 2013-01-15 | 2013-05-22 | 四川农业大学 | Preparation method of microelement zinc-iron raw injection for pig |
-
2014
- 2014-03-24 CN CN201410115216.2A patent/CN103948624B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101978856A (en) * | 2010-11-13 | 2011-02-23 | 兰州大学 | Complex nutrient additive block special for yaks |
| CN103110615A (en) * | 2013-01-15 | 2013-05-22 | 四川农业大学 | Preparation method of microelement zinc-iron raw injection for pig |
Non-Patent Citations (1)
| Title |
|---|
| M.E. SMART ET AL: "Trace Mineral Deficiencies in Cattle: A Review", 《CAN.VET. J.》 * |
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|---|---|
| CN103948624B (en) | 2016-07-06 |
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