CN103918589B - A kind of animal health-care product improving shrimp seedling long-distance transport survival rate - Google Patents

A kind of animal health-care product improving shrimp seedling long-distance transport survival rate Download PDF

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CN103918589B
CN103918589B CN201410167481.5A CN201410167481A CN103918589B CN 103918589 B CN103918589 B CN 103918589B CN 201410167481 A CN201410167481 A CN 201410167481A CN 103918589 B CN103918589 B CN 103918589B
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care product
animal health
shrimp seedling
survival rate
transport
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CN103918589A (en
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翟少伟
张云华
刘伟
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Xiamen Xingmuwei Animal Health Products Co Ltd
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Xiamen Xingmuwei Animal Health Products Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of animal health-care product improving shrimp seedling long-distance transport survival rate, it comprises following component, and every kilogram contains OPC 50-500g, Tea Polyphenols 50-300g, chlorogenic acid 25-200g, montmorillonite 50-150g, carrier 325-500.Described animal health-care product is used for the transport of shrimp seedling can improve shrimp seedling long-distance transport survival rate, and use product of the present invention in breeding water body before transport, effect is better simultaneously.The features such as the raw material of this animal health-care product is easy to get, moderate cost, safe and effective, preparation is simple, addition is low, application prospect is good.

Description

A kind of animal health-care product improving shrimp seedling long-distance transport survival rate
Technical field
The present invention relates to animal health-care product, particularly relate to a kind of animal health-care product improving shrimp seedling long-distance transport survival rate.
Background technology
Penaeus Vannmei formal name used at school Environment of Litopenaeus vannamei Low, is commonly called as white shrimp.The thin body of its shell is fertile, and delicious meat, dressed fish is high, nutritious.Have the advantages such as individuality is large, growth is fast, nutritional need is low, premunition is strong, being the improved seeds of intensive high yielding culture, is also the shrimp species that in current three large cultured prawns in the world, single rate is the highest.To economize in China Guangdong, Guangxi, Fujian, Hainan, Zhejiang, Shandong, Hebei etc. or autonomous region progressively promotes breeding scale, within 2011, become the first kind of Export of Chinese Aquatic Products.
Shrimp seedling is a culture of Penaeus vannamei successfully important ring.In shrimp seedling transportation, unavoidably produce stress reaction, break vivo oxidation and anti-oxidant between poised state, and then affect the aspects such as the digesting and assimilating of animal, immunologic function, breeding, cause poor growth, cause morbidity, even dead, make huge economic loss to shrimp farming.How to slow down the transport stress of shrimp seedling, it is the important topic needing solution badly that prolongation haulage time realizes transport more at a distance.At present be applied to resisting stress patent in feed and products thereof about aquatic livestock more, and for the animal health-care product added in shrimp seedling transport water or additive less.The patent application " a kind of method improving white leg Shrimp seedling long-distance transport survival rate " of CN201210462908.5, the vitamin C adding 100-200 gram in clean fresh water per ton is mixed with transport water.The patent application " a kind of method improving Macrobrachium rosenbergii commercial sized prawn long-distance transport survival rate " of CN201210239673.3, the vitamin C adding 50-100 gram per ton in its transport water.The patent application of CN200910156923.5 " a kind of water body for aquaculture splash type Antistress agent ", mixes at normal temperatures by the composition of following weight portion and makes: glucose 72-85 part; Sulfur amino acid 0.5-4 part; Vitamin 5-18 part; Chlorogenic acid 0.5-2.5 part; Scutelloside 0.5-2.5 part; Citric acid 2-6 part.All report in above-mentioned patent application and alleviate transport stress to a certain extent, improve survival rate.In the health products of water splashing type, the vitamin C that uses as main resisting stress material more at present, and the ascorbic alleviation of occurring in nature stress cause the limited in one's ability of oxidative stress, is difficult at utmost alleviate transport stress; The crowded friction of little consideration transport shrimp body produces the liable to infection problem of wound, and the metabolic waste impact on water quality of shrimp body when transporting also can not be ignored.
Summary of the invention
The object of the present invention is to provide a kind of animal health-care product improving white leg Shrimp seedling long-distance transport survival rate.
For achieving the above object, the invention provides a kind of animal health-care product, it is characterized in that, comprise following component, every kilogram contains OPC 50-500g, Tea Polyphenols 50-300g, chlorogenic acid 25-200g, montmorillonite 50-150g, carrier 325-500.
Preferably include following component, every kilogram contains OPC 150-350g, Tea Polyphenols 100-300g, chlorogenic acid 75-200g, montmorillonite 75-150g, carrier 325-400.
More preferably comprise following component, every kilogram contains OPC 275-350g, Tea Polyphenols 150-300g, chlorogenic acid 100-200g, montmorillonite 100-150g, carrier 335-400.
Described animal health-care product, is characterized in that, carrier is glucose or glauber salt.
Described animal health-care product is used for the purposes of shrimp seedling transport.Described shrimp seedling is white leg Shrimp seedling.
Described purposes, is characterized in that, comprises and transport water or breeding water being dissolved by described animal health-care product, the step of then evenly splashing.
Described purposes, is characterized in that, the consumption of described animal health-care product is the dosage 1-5g that splashes in every cubic metre of breeding water body; Transport every cubic metre, water body is 3-8g.
The present invention, by having the integrated assembling of various active material of strong anti-oxidative activity, antibacterial, adsorption activity, plays synergistic effect, improves Penaeus Vannmei long-distance transport survival rate to greatest extent.Specifically comprise following three aspects: (1) utilizes antioxidation activity to be better than the OPC of vitamin C tens times, Tea Polyphenols crosses polyradical to remove in transport stress, alleviates oxidative stress and harm thereof; (2) utilize the function of OPC, Tea Polyphenols and chlorogenic acid broad-spectrum antiseptic, trauma infection contamination when avoiding shrimp body to transport, prevents the infringement of pathogen; (3) utilize the metabolic waste that there is strong adsorbing montmorillonite and produce when transporting to adsorb shrimp body, purify water.
In the present invention, described OPC is a kind of bioflavonoid having special molecular structure.It is combined into by the catechin of varying number or epicatechin.The simplest OPC is the dimer that catechin or epicatechin or catechin and epicatechin are formed, and the glucosidase procyanidins distribution of two ~ pentamer is the widest, and research and apply is maximum.Be the most effective natural of removing people interior free yl of generally acknowledging in the world at present, domestic OPC generally extracts from grape pip.OPC (grape seed extract) is a kind of new and effective antioxidant, is the most potent found so far free radical scavenger, has very strong activity in vivo.Experiment proves, the Green Tea Extract oxidability of OPC is 50 times of vitamin E, ascorbic 20 times, and absorption can play a role rapidly rapidly completely.In addition, also there is broad-spectrum antiseptic, adjusting blood lipid, anti-inflammatory, antifatigue, delay senility isoreactivity.
Described Tea Polyphenols extracts by tealeaves, is the general name of polyphenols, comprises flavanol compound, anthocyanin class, flavonoids, flavonols and phenolic acid class etc.The most important with flavanol (catechin) class, account for the 60%-80% of Polyphenols total amount.Tea Polyphenols has very strong antioxidation, its oxidation resistance be Prof. Du Yucang antioxidant BHT, BHA 4-6 doubly, doubly, ascorbic 5-10 is doubly for the 6-7 of vitamin E.In addition, also have broad-spectrum antiseptic, hypotensive, blood sugar and blood fat, improve combined immune power and eliminate heavy metal, radiation hazradial bundle, beauty treatment delays senility isoreactivity.
Described chlorogenic acid is the depside generated by caffeic acid and chinic acid, mainly has broad-spectrum antiseptic, antiviral characteristic, increases leukocyte, hepatic cholagogic, antitumor, hypotensive, reducing blood lipid, the effect such as scavenging free radicals and stimulating central nervous system system in addition.Chlorogenic acid is extensively present in high dicotyledon and pteridophyte, mainly extracts from honeysuckle or the bark of eucommia.
Described montmorillonite, also known as montmorillonite, be a kind of alumino-silicate, its main component is octahedra hectorite microparticle, meets pharmaceutical montmorillonite national standard (WS1-(X-165)-2004Z).It has good suction-operated to Escherichia coli, comma bacillus, campylobacter jejuni, staphylococcus aureus and rotavirus and cholate; Fixation is have to bacterial poison; Also can reduce in transport stress defecation, some harmful substances of discharging in absorption shrimp seedling transportation.
All commercially, grinding particle size is all more than 80 orders for the raw material that the present invention relates to.Animal health-care product of the present invention can directly apply in culture of Penaeus vannamei and transport water body, first transport water or breeding water is dissolved, then evenly splash during use.Dosage of splashing is the dosage 1-5g that splashes in white shrimp every cubic metre breeding water body; Transport every cubic metre, water body is 3-8g.
Beneficial effect of the present invention is:
(1) according to the physilogical characteristics of white leg Shrimp seedling In transit, play the various active material synergistic effect with strong anti-oxidative activity, antibacterial, adsorption activity, a kind of animal health-care product that can improve white leg Shrimp seedling long-distance transport survival rate is provided;
(2) apply formula of the present invention, effectively can improve the survival rate after the transport of shrimp seedling, in breeding water body, use product of the present invention before transport, effect is better;
(3) raw material of this animal health-care product be easy to get, moderate cost, the feature such as safe and effective, preparation is simple, addition is low, application prospect is good.
Embodiment
Be described below in detail embodiments of the invention, the example of described embodiment is intended to for explaining the present invention, and can not be interpreted as limitation of the present invention.Unreceipted concrete technology or condition person in embodiment, according to the technology described by the document in this area or condition or carry out according to product description.Agents useful for same or the unreceipted production firm person of instrument, being can by the conventional products of commercial acquisition.
Following table is the weight (unit: gram) of various composition in per kilogram animal health-care product obtained in embodiment 1-5.
Table 1: the weight (unit: gram) of various composition in per kilogram animal health-care product obtained in embodiment 1-5
Raw material Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Embodiment 5
OPC 350 275 500 150 50
Tea Polyphenols 150 175 50 100 300
Chlorogenic acid 115 75 25 200 100
Montmorillonite 50 75 100 150 50
Glucose 335 0 325 400 0
Glauber salt 0 400 0 0 500
embodiment 1:the preparation of animal health-care product
Formula: in table 1
Preparation method: after various raw material is proportionally weighed, drops into mixer and mixes packing and get final product.
embodiment 2:
Formula: in table 1
Preparation method: with embodiment 1.
embodiment 3:
Formula: in table 1
Preparation method: with embodiment 1.
embodiment 4:
Formula: in table 1
Preparation method: with embodiment 1.
embodiment 5:
Formula: in table 1
Preparation method: with embodiment 1.
embodiment 6: compliance test result is tested
First transport water or breeding water are dissolved during use, then evenly splashed.
Test one: establish three groups, be respectively control group, vitamin C group and animal health-care product group.Wherein control group transport water does not add any health products, adds 4mg stable vitamin C in the defeated water of the every elevator of vitamin C group, adds this animal health-care product of 5mg (embodiment 1 product) in the defeated water of the every elevator of experimental group.Each assembling three bags of shrimp seedlings, often packed enter white leg Shrimp seedling 6000 tail of 0.7cm after desalination.The transport water yield of each shrimp seedling special double-layer oxygen bag (specification is 42cm 72cm) is 1/3rd of oxygen bag volume, fills oxygen and causes it in heaving state.With the transport of air-conditioning thermal car, temperature controls at about 23 degree.From Xin Dian town, Xiangan District, Xiamen City, Liu Wudian village is transported to Fuqing City San Shan town Jia Rucun, haulage time 4 hours.The survival rate of control group, vitamin C group and animal health-care product group is respectively 68.6 ± 2.55%, and 75.5 ± 3.28% and 93.60 ± 2.10%.Through SPSS(17.0) statistical software carries out variance analysis and Multiple range test, and the difference in three groups between any two groups is all significantly (P < 0.05).
Test two: establish three groups, be respectively control group, vitamin C group and animal health-care product group.Wherein control group transport water does not add any health products, adds 3mg stable vitamin C in the defeated water of the every elevator of vitamin C group, adds this animal health-care product of 3mg (embodiment 3 product) in the defeated water of the every elevator of animal health-care product group.Each assembling three bags of shrimp seedlings, often packed enter white leg Shrimp seedling 5000 tail of 0.8cm after desalination.The transport water yield of each shrimp seedling special double-layer oxygen bag (specification is 42cm 72cm) is 1/3rd of oxygen bag volume, fills oxygen and causes it in heaving state.With the transport of air-conditioning thermal car, temperature controls at about 22 degree.From Xin Dian town, Xiangan District, Xiamen City, Liu Wudian village is transported to Dongshan County Qian Lou town, Zhangzhou City, haulage time 4 hours.The survival rate of control group, vitamin C group and animal health-care product group is respectively 65.7 ± 3.06%, and 74.2 ± 2.78% and 92.4 ± 3.10%.Through SPSS(17.0) statistical software carries out variance analysis and Multiple range test, and the difference in three groups between any two groups is all significantly (P < 0.05).
Test three: establish four groups, be respectively control group, animal health-care product group 1, animal health-care product group 2, animal health-care product 3.Wherein control group transport water does not add any health products, this animal health-care product of 5mg (embodiment 4 product) is added in the every defeated water of elevator of animal health-care product group 1, the shrimp seedling of animal health-care product group 2 before shipping one week every mu of shrimp pond is splashed this animal health-care product of 1mg (embodiment 4 product) every other day, adds this animal health-care product of 5mg (embodiment 4 product) in the defeated water of every elevator; The shrimp seedling of animal health-care product group 3 before shipping one week every mu of shrimp pond is splashed this animal health-care product of 5mg (embodiment 5 product) every other day, adds this animal health-care product of 8mg (embodiment 5 product) in the defeated water of every elevator.Each assembling three bags of shrimp seedlings, often packed enter white leg Shrimp seedling 7000 tail of 0.7cm after desalination.The transport water yield of each shrimp seedling special double-layer oxygen bag (specification is 42cm 72cm) is 1/3rd of oxygen bag volume, fills oxygen and causes it in heaving state.With the transport of air-conditioning thermal car, temperature controls at about 23 degree.Between to be transported to Tuo Pu town, Shan morning market gold flat district from Liu Wudian village, Xin Dian town, Xiangan District, Xiamen City, haulage time 5 hours.The survival rate of control group, vitamin C group and animal health-care product group is respectively 63.1 ± 2.21%, and 87.8 ± 3.43%, 92.60 ± 3.15% and 92.2 ± 2.85%.Through SPSS(17.0) statistical software carries out variance analysis and Multiple range test, and each animal health-care product composition motility rate is all significantly higher than control group (P < 0.05); Animal health-care product group 2 and animal health-care product group 3 are significantly higher than animal health-care product group 1(P < 0.05), but the difference remarkable (P > 0.05) between animal health-care product group 2 and animal health-care product group 3.
As can be seen from above-mentioned test, animal health-care product of the present invention of splashing in transport water body can improve the survival rate of Penaeus Vannmei transport, and Be very effective is better than vitamin C, and breeding water body these health products effect of splashing is better before shipping.
Although illustrate and describe embodiments of the invention above, be understandable that, above-described embodiment is exemplary, can not be interpreted as limitation of the present invention, those of ordinary skill in the art can change above-described embodiment within the scope of the invention when not departing from principle of the present invention and aim, revising, replacing and modification.

Claims (8)

1. improve an animal health-care product for shrimp seedling long-distance transport survival rate, it is characterized in that, comprise following component, every kilogram contains OPC 50-500g, Tea Polyphenols 50-300g, chlorogenic acid 25-200g, montmorillonite 50-150g, carrier 325-500g.
2. improve the animal health-care product of shrimp seedling long-distance transport survival rate described in claim 1, it is characterized in that, comprise following component, every kilogram contains OPC 150-350g, Tea Polyphenols 100-300g, chlorogenic acid 75-200g, montmorillonite 75-150g, carrier 325-400g.
3. described in claim 1, improve the animal health-care product of shrimp seedling long-distance transport survival rate, it is characterized in that, comprise following component, every kilogram contains OPC 275-350g, Tea Polyphenols 150-300g, chlorogenic acid 100-200g, montmorillonite 100-150g, carrier 335-400g.
4. the animal health-care product of the arbitrary described raising shrimp seedling long-distance transport survival rate of claim 1-3, it is characterized in that, carrier is glucose or glauber salt.
5. the animal health-care product of the arbitrary described raising shrimp seedling long-distance transport survival rate of claim 1-3 is used for the purposes of shrimp seedling transport.
6. the purposes of claim 5, is characterized in that, described shrimp seedling is white leg Shrimp seedling.
7. purposes according to claim 5, is characterized in that, comprises and transport water or breeding water being dissolved by described animal health-care product, the step of then evenly splashing.
8. purposes according to claim 7, is characterized in that, the consumption of described animal health-care product is the dosage 1-5g that splashes in every cubic metre of breeding water body; Transport every cubic metre, water body is 3-8g.
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