CN103907787A - Pig feed and preparation method thereof - Google Patents
Pig feed and preparation method thereof Download PDFInfo
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- CN103907787A CN103907787A CN201410144997.8A CN201410144997A CN103907787A CN 103907787 A CN103907787 A CN 103907787A CN 201410144997 A CN201410144997 A CN 201410144997A CN 103907787 A CN103907787 A CN 103907787A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract
The invention relates to a pig feed and a preparation method thereof. The comprises the following steps: 1. sequentially carrying hot water treatment and centrifugation on collected wet microalga thallus to obtain a supernate A and a precipitate A; 2. sequentially carrying out enzyme addition and centrifugation on the precipitate A to obtain a supernate B and a precipitate B; 3. mixing the supernate A and supernate B, concentrating, adding 4 DEG C cold ethanol, standing for more than 12 hours, and filtering the mixed solution to obtain a crude polysaccharide precipitate; 4. adding water to dissolve the crude polysaccharide precipitate obtained in the step 3, carrying out deproteinization treatment, separating to obtain a protein precipitate and a deproteinized polysaccharide solution, carrying out freeze-drying or drying on the protein precipitate to obtain a crude protein product; 5. separating an intermediate thallus containing high-protein products from the precipitate B; and mixing the following components in parts by mass to obtain the pig feed: 5-10 parts of obtained crude protein product and/or 5-10 parts of high-protein product obtained by drying the intermediate thallus, 61-65 parts of corn, 1-6 parts of bran, 12-25 parts of bean pulp, 1-16 parts of yellow wine vinasse, 0.1-0.5 part of rock powder, 0.01-0.1 part of sodium bicarbonate, 1-1.6 parts of calcium hydrogen phosphate and 0.01-0.05 part of flavorant.
Description
Technical field
The present patent application is December 31 2012 applying date, and application number is: 201210588607.7, and name is called the divisional application of the application for a patent for invention of " a kind of pig feed and preparation method thereof ".The present invention relates to a kind of pig feed and preparation method thereof, especially a kind of can be for edible pig feed of pig, ox or sheep and preparation method thereof.
Background technology
Prevailing along with scientific cultivation, people have had more deep awareness and understanding for the cultivation of domestic animal, how to improve the survival rate of domestic animal, reduce the generation of disease, and accelerate its growth rate and improve meat quality, be the urgent inquisitive problems of numerous cultivation dealers.
As everyone knows, 60 kilogram one of the body weight of pig delivered for sale for the stage of fattening, and this function of each organ, system of pig all perfect gradually in stage, and especially digestive system has had very great development, and the abilities of digestive and absorption of all feeds is all greatly improved.Nervous system and body resistance to external world also progressively improves, and this adipose tissue growth of pig vigorous in stage, but now lean meat percentage reduces, carcass quality variation.The needs of protein are more complicated, in order to obtain best fattening effect, not only to meet the demand of albumen quality, also to consider balance between must amino acid and the equilibrium of utilization rate and minerals and vitamins, if feed collocation is unreasonable, cannot meet the growth demand of large pig.
Existing pig feed is all to come from cereal crops, does not use algae as raw material, has caused a large amount of foodstuff wastes, is unfavorable for the demand of human survival.
Summary of the invention
The present invention has designed a kind of pig feed and preparation method thereof, and the technical problem of its solution is that (1) need to be used a large amount of dregs of beans and corn in order to guarantee the protein content in existing pig feed, and is difficult for fully being absorbed by animal.(2) existing pig feed is all to come from cereal crops, does not come from algae, has caused a large amount of foodstuff wastes, is unfavorable for the demand of human survival.
In order to solve the technical problem of above-mentioned existence, the present invention has adopted following scheme:
A kind of pig feed preparation method, comprises the following steps:
Step 1: micro-algae or micro-algae wet thallus are passed through to hot water treatment and centrifugal treating successively, obtain supernatant A and precipitate A; Wherein, the mass ratio of hot water and micro-algae or micro-algae wet thallus is 20-30:1;
Step 2: precipitate A is passed through enzyme-added processing and centrifugal treating successively, obtains supernatant B and deposit B; Wherein, the mass ratio of precipitate A and enzyme is 40-100:1;
Step 3: will supernatant A and supernatant B concentrate after mixing, and add 4 ℃ of cold ethanol and mix more than 12 hours, mixed liquor is filtered and obtains thick polysaccharide precipitation; Wherein, the cumulative volume of supernatant A and supernatant B is concentrated to 1/5-1/4 of original volume, and the volume that adds cold ethanol is concentrated rear supernatant A and supernatant B cumulative volume 3-5 times;
Step 4: in step 3, the thick polysaccharide precipitation of gained is dissolved in water, de-albumen processing, is separated into albumen precipitation and Deproteinated polysaccharide solution, and albumen precipitation obtains crude protein product by freeze drying or oven dry; Polysaccharide solution after de-albumen can decolour and obtain refining polysaccharide;
Step 5: after regulating pH value to be 10-12 the bacteria suspension of gained deposit B in step 2,70-80 ℃ of insulation 1-2h, then add the sodium chloride solution of 0.8% concentration and the mixed liquor of n-hexane and ethanol, finally make n-hexane: ethanol: the volume ratio of water is 2:4:1.5, concussion 10min, leaves standstill and is divided into three layers and is followed successively by from top to bottom: n-hexane phase, middle thalline phase and alcohol water; N-hexane can obtain uranidin mutually;
Step 6: be mixed and made into pig feed according to following mass ratio: in the middle of the crude protein product that 5-10 part step 4 obtains and/or 5-10 part step 5, thalline is through dried high protein product, 61-65 part corn, 1-6 part wheat bran, 12-25 part dregs of beans, 1-16 part yellow wine lees, 0.1-0.5 part stone flour, 0.01-0.1 part sodium bicarbonate, 1-1.6 part calcium monohydrogen phosphate and 0.01-0.05 part essence.
Further, micro-algae is for being chlorella, spirulina or grid algae; Micro-algae wet thallus is chlorella thalline, spirulina thalline or grid phycomycete body.
Further, the hot water temperature in step 1 and processing time are respectively 90-100 ℃ and 0.5-2h.
Further, the enzyme in step 2 is selected from one or more combinations in neutral proteinase, alkali protease and cellulase; Cellulose enzyme activity is 1200-1500U/g, and neutral proteinase enzyme is lived as 59000-60000U/g, and the work of alkali protease enzyme is 2 × 10
5-2.02 × 10
5u/g.
Further, in the pig feed of the final gained of step 6, also add trace element.
Further, the mass fraction of trace element is according to following ratio: 0.4-0.6 portion of terramycin, 0.2-1.5 part copper sulphate, 0.2-1.5 part ferrous sulfate, 0.3-1.8 part zinc sulfate and 1-2 portions of salt.
This invention also comprises by above-mentioned 6 kinds of pig feeds that preparation method makes.
This pig feed and preparation method thereof, compared with traditional pig feed and preparation method thereof, has following beneficial effect:
(1) the present invention by isolating crude protein product and high protein product from algae, make protein content in pig feed higher than conventional feed, for animal feeding provides enough protein, the source of this protein and algae simultaneously, thereby can reduce corn and dregs of beans use amount.
(2) pig feed of the present invention is not only applicable to in-pig, milking sow, growth pig, fattening pig and large pig, can allow their quick, healthy growths, but also reduce the cost of pig feed, because vinasse are contained in this pig feed the inside, vinasse can replace part corn, dregs of beans, saved grain, and pig also enjoys a lot to eat the feed that contains vinasse.
(3) the present invention designs and uses hot water treatment as preprocessing means, increases cell permeability, suitably destroys cell wall structure, reduces the use amount of enzyme process enzyme process action time and enzyme, by hot-water extraction and enzymolysis, obtains product polysaccharide and a small amount of crude protein.
(4) the present invention, except producing pig feed, can be decoloured in the polysaccharide solution after its by-product pint albumen and obtain refining polysaccharide.
(5) the present invention is except producing pig feed, and its byproduct n-hexane can obtain uranidin in mutually.
(6) in the present invention, increase trace element and can meet the needs of pig growth and development to various mineral matter elements, especially can make piggy ramp.
The specific embodiment
In conjunction with the following example, the present invention will be further described:
Embodiment 1: select chlorella
Chlorella zymotic fluid, with the centrifugal 10min of 4000rpm/min, is removed to supernatant and obtained chlorella wet thallus, take chlorella wet thallus 1.0g in 100ml conical flask, add 25ml deionized water, 90 ℃ of heating 1.5h, cooling, centrifugal supernatant A and the precipitate A of obtaining.Take 0.065g cellulase, 0.035g neutral proteinase, cellulose enzyme activity is 1200U/g, and neutral proteinase enzyme is lived as 60000U/g, adds the citric acid-sodium citrate buffer of pH=5, and pH=5 is the pH reaction system of optimum enzyme effect selected in experiment.Be settled to 100ml, get 25ml enzyme solutions and join in chlorella wet thallus precipitate A, hydrolysis temperature is 42 ℃, stirs 4h, 90 ℃ of enzyme 10min that go out.Suspension after hydrolysis is carried out to centrifugal 10min under 4000r/min, obtain supernatant B and deposit B.
Merge supernatant A and supernatant B, concentrated this supernatant mixed liquor, to 1/5 of original volume, adds 4 ℃ of concussions of cold ethanol of 3 times of volumes to mix, and leaves standstill 12h, filters to obtain thick polysaccharide precipitation.
Get the thick polysaccharide precipitation of the water-soluble solution of 10ml, with trichloroacetic acid adjusting pH to 3, mix, leave standstill 2h, add the n-butanol of 15 times of trichloroacetic acid volumes, stir, leave standstill 1h, centrifugal, the aqueous solution reclaims polysaccharide, repeats 2 times.Polysaccharide extract rate 82.3%.Water dissolves polysaccharide, with the flow velocity of 2BV/h, the thick polysaccharide solution of 2.5mg/ml is carried out to adsorption bleaching, records pigment removal efficiency 92.4%, and polysaccharide retention rate is 88.97%.When pH=3, protein solubility is lower and polysaccharide loss is less, is peak optimization reaction system.The use of n-butanol is also in order to reduce the water-soluble of protein, thus crude protein product and separation of polysaccharides.Use separately TCA method also passable herein, increase the number of times that repeats extraction.
It is 11 that the bacteria suspension of deposit B regulates pH with the NaOH of 2mol/L, 70 ℃ of reaction 60min, cooling, adding appropriate 0.8% sodium chloride solution and the mixed liquor n-hexane of n-hexane and ethanol and the volume ratio of ethanol is 2:4, makes final n-hexane: ethanol: the volume ratio of water is 2:4:1.5, concussion 10min, collect n-hexane phase, add the n-hexane of initial 1/3 volume to repeat twice, n-hexane phase A adds anhydrous sodium sulfate and removes minor amount of water, reclaims solvent and obtains the uranidin such as carrotene again.Its crude protein content of high protein product after drying of thalline that intermediate layer suspends is 38.7%, and 18 water solution amino acid content is abundant, can be used as be rich in albumen completely, amino acid whose composition is used as protein feed becomes the part substitute of animal's diet feed.
Choose 5 parts of crude protein products, 10 parts of high protein products, 50 parts of corns, 8 parts of wheat brans and 15 parts of dregs of beans, by agitating device, they are fully mixed, can become the pig feed of high-quality.
Choose 10 parts of crude protein products, 10 parts of high protein products, 65 parts of corns, 6 parts of wheat brans, 25 parts of dregs of beans, 6 parts of yellow wine lees, 0.1 part of stone flour, 0.1 part of sodium bicarbonate, 1 part of calcium monohydrogen phosphate and 0.01 part of essence.By agitating device, they are fully mixed, can become the pig feed of high-quality.
Embodiment 2: select grid algae
Grid algae zymotic fluid, with the centrifugal 10min of 4000rpm/min, is removed to supernatant and obtained grid phycomycete body, take grid algae wet thallus 1.0g in 100ml conical flask, add 25ml deionized water, 90 ℃ of heating 1.5h, cooling, centrifugal supernatant A and the precipitate A of obtaining.Take 0.075g cellulase, 0.025g alkali protease, cellulose enzyme activity is 1200U/g, the work of alkali protease enzyme is 2 × 10
5u/g, add the citric acid-sodium citrate buffer of pH=4 to be settled to 100ml, getting 25ml enzyme solutions joins in grid algae wet thallus precipitate A, hydrolysis temperature is 40 ℃, stir 4h, 90 ℃ of enzyme 10min that go out, carry out centrifugal 10min under 4000r/min by the suspension after hydrolysis, obtain supernatant B and deposit B.
Merge supernatant A and supernatant B, concentrated this supernatant mixed liquor, to 1/5 of original volume, adds 4 ℃ of concussions of cold ethanol of 3 times of volumes to mix, and leaves standstill 12h, filters to obtain thick polysaccharide precipitation.
Get the thick polysaccharide precipitation of the water-soluble solution of 10ml, with trichloroacetic acid adjusting pH to 3, mix, leave standstill 2h, add the n-butanol of 15 times of trichloroacetic acid volumes, stir, leave standstill 1h, centrifugal, the aqueous solution reclaims polysaccharide, repeats 2 times.Polysaccharide extract rate 82.3%.Water dissolves polysaccharide, with the flow velocity of 2BV/h, the thick polysaccharide solution of 2.5mg/ml is carried out to adsorption bleaching, records pigment removal efficiency 92.4%, and polysaccharide retention rate is 88.97%.When pH=3, protein solubility is lower and polysaccharide loss is less, is peak optimization reaction system.The use of n-butanol is also in order to reduce the water-soluble of protein, thus crude protein product and separation of polysaccharides.Use separately TCA method also passable herein, increase the number of times that repeats extraction.
It is 11 that the bacteria suspension of deposit B regulates pH with the NaOH of 2mol/L, 70 ℃ of reaction 60min, cooling, adding appropriate 0.8% sodium chloride solution and the mixed liquor n-hexane of n-hexane and ethanol and the volume ratio of ethanol is 2:4, makes final n-hexane: ethanol: the volume ratio of water is 2:4:1.5, concussion 10min, collect n-hexane phase, add the n-hexane of initial 1/3 volume to repeat twice, n-hexane phase A adds anhydrous sodium sulfate and removes minor amount of water, reclaims solvent and obtains the uranidin such as carrotene again.Its crude protein content of high protein product after drying of thalline that intermediate layer suspends is 38.7%, and 18 water solution amino acid content is abundant, can be used as be rich in albumen completely, amino acid whose composition is used as protein feed becomes the part substitute of animal's diet feed.
Choose 5 parts of crude protein products and 5 parts of high protein products, 61 parts of corns, 3 parts of wheat brans, 20 parts of dregs of beans, 10 parts of yellow wine lees, 0.3 part of stone flour, 0.02 part of sodium bicarbonate, 1 part of calcium monohydrogen phosphate and 0.02 essence.By agitating device, they are fully mixed, can become the pig feed of high-quality.
Embodiment 3: select spirulina
Spirulina zymotic fluid, with the centrifugal 10min of 4000rpm/min, is removed to supernatant and obtained spiral thalline, take spiral wet thallus 0.8g in 100ml conical flask, add 25ml deionized water, 90 ℃ of heating 1.5h, cooling, centrifugal supernatant A and the precipitate A of obtaining.Take 0.067g neutral proteinase, alkali protease enzyme is lived as 60000U/g, adds the citric acid-sodium citrate buffer of pH=7 to be settled to 100ml, gets 20ml enzyme solutions to join in grid algae wet thallus, and hydrolysis temperature is 42 ℃, stirs 6h, 90 ℃ of enzyme 10min that go out.Suspension after hydrolysis is carried out to centrifugal 10min under 4000r/min, obtain supernatant B and deposit B.
Merge supernatant A and supernatant B, concentrated this supernatant mixed liquor, to 1/5 of original volume, adds 4 ℃ of concussions of cold ethanol of 3 times of volumes to mix, and leaves standstill 12h, filters to obtain thick polysaccharide precipitation.
Get the thick polysaccharide precipitation of the water-soluble solution of 10ml, with trichloroacetic acid adjusting pH to 3, mix, leave standstill 2h, add the n-butanol of 15 times of trichloroacetic acid volumes, stir, leave standstill 1h, centrifugal, the aqueous solution reclaims polysaccharide, repeats 2 times.Polysaccharide extract rate 82.3%.Water dissolves polysaccharide, with the flow velocity of 2BV/h, the thick polysaccharide solution of 2.5mg/ml is carried out to adsorption bleaching, records pigment removal efficiency 92.4%, and polysaccharide retention rate is 88.97%.When pH=3, protein solubility is lower and polysaccharide loss is less, is peak optimization reaction system.The use of n-butanol is also in order to reduce the water-soluble of protein, thus crude protein product and separation of polysaccharides.Use separately TCA method also passable herein, increase the number of times that repeats extraction.
It is 11 that the bacteria suspension of deposit B regulates pH with the NaOH of 2mol/L, 70 ℃ of reaction 60min, cooling, adding appropriate 0.8% sodium chloride solution and the mixed liquor n-hexane of n-hexane and ethanol and the volume ratio of ethanol is 2:4, makes final n-hexane: ethanol: the volume ratio of water is 2:4:1.5, concussion 10min, collect n-hexane phase, add the n-hexane of initial 1/3 volume to repeat twice, n-hexane phase A adds anhydrous sodium sulfate and removes minor amount of water, reclaims solvent and obtains the uranidin such as carrotene again.Its crude protein content of high protein product after drying of thalline that intermediate layer suspends is 38.7%, and 18 water solution amino acid content is abundant, can be used as be rich in albumen completely, amino acid whose composition is used as protein feed becomes the part substitute of animal's diet feed.
Choose 10 parts of high protein products, 65 parts of corns, 5 parts of wheat brans, 25 parts of dregs of beans, 10 parts of yellow wine lees, 0.5 part of stone flour, 0.1 part of sodium bicarbonate, 1.6 portions of calcium monohydrogen phosphates, 0.05 essence, 0.6 part of terramycin, 0.2 part of copper sulphate, 0.2 part of ferrous sulfate, 1.8 parts of zinc sulfate and 1 portion of salt.By agitating device, they are fully mixed, can become the pig feed of high-quality.
The present invention has been carried out to exemplary description above in conjunction with the embodiments; obvious realization of the present invention is not subject to the restrictions described above; as long as the various improvement that adopted method design of the present invention and technical scheme to carry out; or without improving, design of the present invention and technical scheme are directly applied to other occasion, all in protection scope of the present invention.
Claims (4)
1. a pig feed preparation method, comprises the following steps:
Step 1: micro-algae or micro-algae wet thallus are passed through to hot water treatment and centrifugal treating successively, obtain supernatant A and precipitate A; Wherein, the mass ratio of hot water and micro-algae or micro-algae wet thallus is 20-30:1, and the hot water temperature in step 1 and processing time are respectively 90-100 ℃ and 0.5-2h;
Step 2: precipitate A is passed through enzyme-added processing and centrifugal treating successively, obtains supernatant B and deposit B; Wherein, the mass ratio of precipitate A and enzyme is 40-100:1;
Step 3: will supernatant A and supernatant B concentrate after mixing, and add 4 ℃ of cold ethanol and mix more than 12 hours, mixed liquor is filtered and obtains thick polysaccharide precipitation; Wherein, the cumulative volume of supernatant A and supernatant B is concentrated to 1/5-1/4 of original volume, and the volume that adds cold ethanol is concentrated rear supernatant A and supernatant B cumulative volume 3-5 times;
Step 4: in step 3, the thick polysaccharide precipitation of gained is dissolved in water, de-albumen processing, is separated into albumen precipitation and Deproteinated polysaccharide solution, and albumen precipitation obtains crude protein product by freeze drying or oven dry;
Step 5: after regulating pH value to be 10-12 the bacteria suspension of gained deposit B in step 2,70-80 ℃ of insulation 1-2h, then add the sodium chloride solution of 0.8% concentration and the mixed liquor of n-hexane and ethanol, finally make n-hexane: ethanol: the volume ratio of water is 2:4:1.5, concussion 10min, leaves standstill and is divided into three layers and is followed successively by from top to bottom: n-hexane phase A, middle thalline phase and alcohol water;
Step 6: be mixed and made into pig feed according to following mass ratio: in the middle of the crude protein product that 5-10 part step 4 obtains and/or 5-10 part step 5, thalline, through dried high protein product, 61-65 part corn, 1-6 part wheat bran, 12-25 part dregs of beans, 1-16 part yellow wine lees, 0.1-0.5 part stone flour, 0.01-0.1 part sodium bicarbonate, 1-1.6 part calcium monohydrogen phosphate and 0.01-0.05 part essence, also adds trace element in the pig feed of the final gained of step 6; The mass fraction of trace element is according to following ratio: 0.4-0.6 portion of terramycin, 0.2-1.5 part copper sulphate, 0.2-1.5 part ferrous sulfate, 0.3-1.8 part zinc sulfate and 1-2 portions of salt.
2. pig feed preparation method according to claim 1, is characterized in that: micro-algae is for being chlorella, spirulina or grid algae; Micro-algae wet thallus is chlorella thalline, spirulina thalline or grid phycomycete body.
3. pig feed preparation method according to claim 1, is characterized in that: the enzyme in step 2 is selected from one or more combinations in neutral proteinase, alkali protease and cellulase; Cellulose enzyme activity is 1200-1500U/g, and neutral proteinase enzyme is lived as 59000-60000U/g, and the work of alkali protease enzyme is 2 × 10
5-2.02 × 10
5u/g.
4. the pig feed making according to any one preparation method of claim 1 to 6.
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| CN201410144997.8A CN103907787B (en) | 2012-12-31 | 2012-12-31 | One boar food and preparation method thereof |
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| CN201210588607.7A CN103005218B (en) | 2012-12-31 | 2012-12-31 | Pig feed and preparation method thereof |
| CN201410144997.8A CN103907787B (en) | 2012-12-31 | 2012-12-31 | One boar food and preparation method thereof |
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| CN201410144996.3A Expired - Fee Related CN103907786B (en) | 2012-12-31 | 2012-12-31 | One boar food and preparation method thereof |
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| CN110279038A (en) * | 2019-07-06 | 2019-09-27 | 曾楚华 | One kind containing phycocyanin pannage and preparation method thereof |
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| CN103330071A (en) * | 2013-06-19 | 2013-10-02 | 凤台县盛鹏养殖有限公司 | Pig feed containing seasoning additive and preparation method thereof |
| CN107080045A (en) * | 2017-05-25 | 2017-08-22 | 甘肃凯源生物技术开发中心 | A kind of healthy pig feed of the cultivation containing chlorella and preparation method |
| CN109463557A (en) * | 2018-09-30 | 2019-03-15 | 宁国市农诚农业发展有限公司 | A kind of high protein antibacterial chicken feed and preparation method thereof |
| CN109588569A (en) * | 2019-01-30 | 2019-04-09 | 南京致润生物科技有限公司 | A kind of animal protein-free source pig daily ration and its application using fermented bean dregs preparation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1452890A (en) * | 2003-05-27 | 2003-11-05 | 王智辉 | Special liquid feed for sucking pig |
| CN1537447A (en) * | 2003-04-16 | 2004-10-20 | 四川省川大三康生物工程股份有限公司 | Composite feed additive without antibiotic |
| CN101904419A (en) * | 2010-07-09 | 2010-12-08 | 湖北汇特生物医药技术有限公司 | Application of algae extract as antibiotic substitute in feed |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1817176A (en) * | 2006-03-09 | 2006-08-16 | 王顺荣 | Pig Feed Formula |
| CN101406241A (en) * | 2007-10-12 | 2009-04-15 | 天津生机集团有限公司 | Green composite feed for livestock and poultry |
| CN101717800B (en) * | 2009-11-06 | 2013-01-23 | 华南理工大学 | Method for producing polyunsaturated fatty acid and protein feed additive by crypthecodinium cohnii |
| CN102113635A (en) * | 2010-12-31 | 2011-07-06 | 马艳荣 | Pig feed additive |
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- 2012-12-31 CN CN201210588607.7A patent/CN103005218B/en active Active
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537447A (en) * | 2003-04-16 | 2004-10-20 | 四川省川大三康生物工程股份有限公司 | Composite feed additive without antibiotic |
| CN1452890A (en) * | 2003-05-27 | 2003-11-05 | 王智辉 | Special liquid feed for sucking pig |
| CN101904419A (en) * | 2010-07-09 | 2010-12-08 | 湖北汇特生物医药技术有限公司 | Application of algae extract as antibiotic substitute in feed |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110279038A (en) * | 2019-07-06 | 2019-09-27 | 曾楚华 | One kind containing phycocyanin pannage and preparation method thereof |
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| CN103907787B (en) | 2015-12-09 |
| CN103005218B (en) | 2014-05-14 |
| CN103907786A (en) | 2014-07-09 |
| CN103907786B (en) | 2015-12-30 |
| CN103005218A (en) | 2013-04-03 |
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Inventor after: Tang Baolin Inventor after: Zhao Zhiming Inventor after: Zhang Runpei Inventor before: Liu Yunxiang |
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Effective date of registration: 20170504 Address after: 529000 Guangdong Province, Jiangmen City Xinhui District, Da Ze Zhen Wen Long Cun Yang core area Patentee after: JIANGMEN HAIHE FEED Co.,Ltd. Address before: 322000 Zhejiang Province, Yiwu city streets off South Lane 7 Lane 6 Patentee before: Yiwu Hean Agricultural Technology Co.,Ltd. |
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Denomination of invention: A pig feed and its preparation method Effective date of registration: 20230317 Granted publication date: 20151209 Pledgee: Jiangmen City Sub branch of Postal Savings Bank of China Co.,Ltd. Pledgor: JIANGMEN HAIHE FEED Co.,Ltd. Registration number: Y2023980035221 |