CN103893549B - A kind of Chinese medicine composition of fatigue alleviating and its production and use - Google Patents

Abstract

The present invention relates to Chinese medicine composition of a kind of fatigue alleviating and its production and use, this Chinese medicine composition includes the raw material of following weight proportion: Fructus Lycii 1 50 parts, Rhizoma Polygonati 1 30 parts, Rhizoma Dioscoreae 1 30 parts, Flos Chrysanthemi 1 30 parts, 1 15 parts of Radix Glycyrrhizae, Fructus Mume 1 15 parts and Pericarpium Citri Reticulatae 18 parts.The preparation method of the present invention uses extraction process by water, and application is centrifugal and the technology such as membrance separation, obtains the concentrated liquid of tradition Chinese medicine, or the spray-dried traditional Chinese medicinal extract powder that obtains, adds adjuvant, makes beverage, oral liquid, granule or tablet.The Chinese medicine composition of the present invention has alleviating physical fatigue and asthenopia and contributes to improving memory and the effect of sexual function, clear efficacy, safe without toxic side effect.

Description

A kind of Chinese medicine composition of fatigue alleviating and its production and use

Technical field

The invention belongs to pharmaceutical technology field, be specifically related to a kind of alleviating physical fatigue and asthenopia, Contribute to Chinese medicine composition improving memory and sexual function and its production and use.

Background technology

Along with the rapid change of society, at communication society, computer, television set, mobile phone etc. Video display terminal penetrates into the every nook and cranny of daily life, adds adding of work and rhythm of life Hurry up, increasing people is in sub-health state, and cardinal symptom shows as fatigue, including muscle power Fatigue, mental fatigue and asthenopia etc., affect quality of life.

Modern medicine is thought, fatigue is the local organization caused by many reasons, decreased organ function Or the subjective sensation of general malaise, behavioristics shows as weakening of work capacity.Existing literary composition In offering, tired classification is mainly had following several: according to Producing reason be divided into physical fatigue and Mental fatigue；It is divided into acute fatigue and confirmed fatigue according to the persistent period；Divide according to generating unit For whole body fatigue and local fatigue.Physical fatigue is tired also known as physiological fatigue, muscle fatigue or body Labor.Modern medicine to the search time of physiological fatigue relatively early, begins one's study the eighties in 19th century Sports fatigue just refer to physical fatigue.The 5th international movement that nineteen eighty-two holds in Boston Sports fatigue is defined as by biochemical meeting: organism physiology process can not continue its function specific In level and (or) predetermined exercise intensity can not be maintained.Mental fatigue is also known as Subjective fatigue, essence Mental fatigue labor or psychological fatigue." psychology dictionary " is pointed out when explaining mental fatigue: psychology is tired Labor refers to that the cognition that spirit undue concentration or puzzlement are caused is tired.Mental fatigue mainly shows as Difficult concentrating, thinking difficulty, Vigilance reduces, hypomnesis, hyposexuality etc.. Asthenopia one word is derived by Hellenic expression method, and original text means " weak eye " (eye Unable).Existing most ophthalmology scholar thinks that primary disease is based on the symptom of the conscious eye of patient, eye Or the syndrome that whole body apparatus factors is interweaved with spirit (psychological) factor.Thus the most normal quilt It is referred to as eyestrain's syndrome.The nutrient substance of fatigue alleviating mainly has: saccharide, lipid, albumen Matter, water and electrolyte, trace element, antioxidant includes superoxide dismutase, peroxidating Hydrogen enzyme, acetone acid etc., aminoacid include leucine, tyrosine, aspartic acid, glutamine, Taurine, creatine etc., vitamin includes vitamin C, vitamin E, L-carnitine, B race Vitamin such as B1, B2, B6, B12 etc.；The chemical substance of resisting fatigue is mainly caffeine etc.. On market with the relevant healthcare product of nutrient substance and chemical substance compatibility mainly have red cattle, force health-care, Fill it up with day, Qi Li, pleasure are brave.

Classical Chinese medicine Huangdi's Internal Classics has discussion to fatigue, many referred to as " tired ", " ", " solution Lazy ", " tired thin ", " heavy sensation of the body ", " body weight " etc.." gold is deficient first appeared in Chinese Zhang Zhongjing for a tired word Outline ": " ask and say: what arthralgia due to stagnation of blood obtain it from?Teacher says: husband's rank and honour people's weak bone skin contain, weight because of Tired sweating, shaking frequently when lying in bed, add by mild wind, then obtain it." Tang's Sun Simiao " prescriptions worth thousand gold ": " five kinds of over strain person: says will labor, and two say think of labor, and three say the impairment of the heart caused by overstrain, and four say care-laden and overworked, and five say fatigue ... Its reading, game etc. excessively use mesh person, name says hepatic asthenia." Chinese medicine thinks, muscle power produce with The material base that endurance maintains is the smart qi-blood-body fluid in body, the metaplasia of these materials and defeated cloth Closely related with function of five internal organs；Physical fatigue is in close relations with liver spleen, and asthenopia closes with Liver and kidney Closely, mental fatigue is in close relations with heart spleen in system.Traditional Chinese medical science allaying tiredness main principle be tonification with Conditioning, tonification mainly includes YIN nourishing and YANG invigorating, QI invigorating with enrich blood, nourishing the liver and kidney etc.；Conditioning is main Spleen invigorating to be included is invigorated blood circulation, depressed liver-energy dispersing and QI regulating etc..On market, the relevant healthcare product of Chinese medicine class are mainly with deer The YANG invigorating Chinese medicines such as young pilose antler, Lac regis apis, Radix Ginseng and Radix Panacis Quinquefolii are main.

Therefore, on the one hand, although being currently used for fatigue alleviating, improving memory and the product of sexual function Product of a great variety, but generally there is various shortcoming in these products, as long-term taking holds Untoward reaction such as being overexcited, hormonal readiness is unbalance, the resultant effect for the treatment of conditioning is easily caused to have Limit and expensive etc..On the other hand, at present for can simultaneously alleviating physical fatigue and regarding Fatigue, and improvement is remembered and the tcm product of sexual function and safe without toxic side effect exists demand.

Summary of the invention

In view of this, the invention provides the Chinese medicine composition of the progressive fatigue alleviating of a kind of gentleness And preparation method thereof；It addition, present invention also offers the purposes of this Chinese medicine composition.

In order to realize foregoing invention purpose, according on the one hand, the invention provides fatigue alleviating Chinese medicine composition, it is made up of the active component of following weight portion: Fructus Lycii 1-50 part, Huang Essence 1-30 part, Rhizoma Dioscoreae 1-30 part, Flos Chrysanthemi 1-30 part, Radix Glycyrrhizae 1-15 part, Fructus Mume 1-15 part With Pericarpium Citri Reticulatae 1-8 part.

According to a preferred embodiment, described weight proportion is: Fructus Lycii 2-25 part, Rhizoma Polygonati 2-15 part, Rhizoma Dioscoreae 2-15 part, Flos Chrysanthemi 2-15 part, Radix Glycyrrhizae 2-9 part, Fructus Mume 2-9 part and old Skin 1-5 part.According to another preferred embodiment, described weight proportion is: Fructus Lycii 13 Part, Rhizoma Polygonati 9 parts, Rhizoma Dioscoreae 9 parts, Flos Chrysanthemi 9 parts, 4 parts of Radix Glycyrrhizae, Fructus Mume 4 parts and Pericarpium Citri Reticulatae 2 Part.

Source, nature and flavor, Gui Jing, effect and relevant showing in above-mentioned prescription, to each raw material Generation research is described as follows:

Fructus Lycii: for the drying and ripening fruit of plant of Solanaceae lycium barbarum Lycium barbarum L. Real.Summer, season in autumn two fruit are gathered when taking on a red color, hot-air seasoning, remove carpopodium, or dry in the air to skin After wrinkle, dry, remove carpopodium.Sweet in the mouth, property is put down.Return liver, kidney channel.Effect nourishing the liver and kidney, Replenishing vital essence to improve eyesight." Shennong's Herbal is classified as top grade, record: " in main five, pathogen, hankers disappearing Yearningly；General arthralgia, the hard muscles and bones of clothes, makes light of one's life by commiting suicide the oldest for a long time." Compendium of Material Medica record: " mend vital essence all Deficiency, easy color, bleach, improving eyesight is calmed the nerves, and makes us long-lived.”

Fructus Lycii main functional component is glycine betaine, flavonoid, polysaccharide etc., in nutritional labeling, Proline and arginine content are high, and carotenoid content is high, possibly together with vitamin C precursor 2-O-β-D-Glucose base-L-AA (AA-2 β G).Modern medicine proves, Fructus Lycii has Having antioxidation, defying age, prevent Macular pathological changes, strengthen hemopoietic function, resisting fatigue etc. is multiple Biological activity.{ in grand；The chemical composition of Fructus Lycii and biological activity；" external Medicinal Plants Medicine fascicle ", 2007,22 (2): 51-53 }

Rhizoma Polygonati: for liliaceous plant P. kingianum Polygonatum kingianum Coll.Et Hemsl., Rhizoma Polygonati Polygonatum sibiricumRed. or Polygonatum cyrtonema Hua Polygonatum The dry rhizome of cyrtonema Hua.Different by shape, practise and claim " big Rhizoma Polygonati ", " Caput Gallus domesticus Huang Essence ", " RHIZOMA POLYGONATI ZINGIBERIFORME ".Season in spring and autumn two excavates, and removes fibrous root, cleans, puts in boiling water boiling the hottest Or steam to the saturating heart, it is dried.Sweet in the mouth, property is put down.Return spleen, lung, kidney channel.Effect boosting qi and nourishing yin, Spleen invigorating, lung moistening, kidney tonifying.Compendium of Material Medica is recorded: " mend all void, only cold and heat, replenishing essence marrow.”

Rhizoma Polygonati main functional component is that steroid saponin includes diosgenin, Sarsasapogenin etc., Possibly together with polysaccharide, alkaloid, ketone, phlegmatic temperament etc..In nutritional labeling, arginine and Soviet Union Histidine content is high.Modern study shows, Rhizoma Polygonati has resisting fatigue, improves and improve memory, anti- Oxidation, blood sugar lowering, blood fat reducing etc. acts on.{ Dong Zhicheng thanks Zhaoming, Plumbum preparatium, Li Shunxiang； Rhizoma Polygonati resource, chemical composition and pharmacological research overview；" Central-South pharmacy ", 2012,10 (6): 450-453 }

Rhizoma Dioscoreae: for the dry rhizome of Dioscoreaceae plant Rhizoma Dioscoreae Dioscorea opposita Thunb.. Winter, stem and leaf was excavated after withering, and cut root head, cleaned, and removed crust and fibrous root, was dried, or Take advantage of fresh-cut sheet, be dried；Also have and select loose straight dry Rhizoma Dioscoreae, put in clear water, be dipped into Without the dry heart, vexed, trim two ends, be twisted into plank cylindric, dry, polishing, practise and claim " light Rhizoma Dioscoreae ".Sweet in the mouth, property is put down.Return spleen, lung, kidney channel.Effect spleen reinforcing nourishing the stomach, promote the production of body fluid lung benefiting, The kidney invigorating arresting seminal emission.Shennong's Herbal: be classified as top grade, records: " qi-restoratives is thin for Rhizoma Dioscoreae, main damaging the spleen and stomach, Except cold and heat pathogen.Invigorating middle warmer, physical strength profiting, long muscle.Take normal hearing and eyesight for a long time, make light of one's life by commiting suicide, the most hungry, Prolong life." Compendium of Material Medica record: " kidney tonifying gas, strengthening the spleen and stomach, antidiarrheal dysentery, reduce phlegm saliva, profit Fur.”

Rhizoma Dioscoreae main functional component is steroid saponin, polysaccharide etc., possibly together with allantoin, mucus Matter, sterol, adenosine, choline etc..In nutritional labeling, glutamic acid, aspartic acid and arginine Content is high, containing abundant vitamin C, vitamin B1 and B2.Modern pharmacological research shows, Rhizoma Dioscoreae has enhancing intestinal absorption ability, antioxidation, regulation immunity, blood sugar lowering, blood fat reducing etc. Pharmacologically active.{ Yang Xiu shrimp；Rhizoma Dioscoreae chemical composition and pharmacology activity research progress；" Asia-Pacific tradition Medicine ", 2013,9 (5): 65-66 }

Flos Chrysanthemi: be dried head for feverfew chrysanthemum Chrysanthemum morifolium Ramat. Inflorescence.The 9-11 month gathers when spending in full bloom in batches, dries in the shade or dries, or smoked, steam after dry.Medicine Material is different with processing method by the place of production, is divided into " Flos Chrysanthemi ", " Flos Chrysanthemi ", " Flos Chrysanthemi ", " Flos Chrysanthemi ". Sweet in the mouth, hardship, cold nature.Return lung, Liver Channel.Effect dissipates wind heat clearing away, suppressing the hyperactive liver improving eyesight, heat clearing away solution Poison.Shennong's Herbal is classified as top grade, records: " main all wind, dizziness, swell and ache, and mesh is intended to take off, Lacrimation；The dead flesh of skin, aversion to wind arthralgia chiefly caused by damp pathogen.For a long time clothes profit vim and vigour, make light of one's life by commiting suicide resistance to always, prolong life." " book on Chinese herbal medicine Detailed outline " record: " chrysanthemum Chun Shengxia cyclopentadienyl, Qiu Huadong is real, enjoys four gas, experiences all kinds of dew frost, and leaf is withered Not falling, spend withered not zero, taste is held concurrently sweetness and bitterness, and property is reported gentle.In Bei, body is light, and angle eats also.”

Flos Chrysanthemi main functional component is that flavonoid includes luteoloside, Flos Buddlejae glycosides etc., phenolic acids Including chlorogenic acid, 3,5-O-dicaffeoyl-quinic acid etc., volatile oil include Camphora, Borneolum Syntheticum, Beta-elemenes etc., possibly together with echinacoside, polysaccharide etc..In nutritional labeling, proline and glutamic acid Content is high.Chinese scholars research shows, Flos Chrysanthemi has antioxidation, anti-bacteria and anti-virus, treatment The multiple pharmacologically actives such as coronary heart disease, reduces blood pressure, prevention hyperlipidemia.Jiang Baoping, Xu Lijia, Wang Qiuling, Xiao Peigen；The tradition use of Flos Chrysanthemi and chemical composition and pharmacology activity research are in progress； " contemporary Chinese Chinese medicine ", 2013,15 (6): 523-530 }

Radix Glycyrrhizae: for glycyrrhizic legume Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. Glycyrrhiza inflata Bat. or the dry root of Glycyrrhiza glabra L. Glycyrrhiza glabra L. and root Stem.Spring, season in autumn two excavate, and remove fibrous root, dry.Sweet in the mouth, property is put down.GUIXIN, lung, spleen, Stomach warp.Effect invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription. Shennong's Herbal is classified as top grade, records: " main the five internal organs six mansion cold and heat pathogen；Hard muscles and bones, Long muscle, times power；Incised wound swells；Removing toxic substances.Clothes are made light of one's life by commiting suicide and are prolonged life for a long time." Compendium of Material Medica record: " solve infantile carbuncle infantile convulsion, pathogenic fire reducing pain relieving.”

Radix Glycyrrhizae main functional component is that saponins includes glycyrrhizin, glycyrrhizic acid, enoxolone etc., Flavonoid includes liquirtin, the different glycosides of Radix Glycyrrhizae etc., possibly together with Coumarins, alkaloid, polysaccharide etc.. In nutritional labeling, proline and arginine content are high.Modern clinical research confirms, Radix Glycyrrhizae has Hepatoprotective, antitumor, anti-bacteria and anti-virus, antiulcer, the clinical effect such as pain relieving.{ Tian Wu Raw；The chemical composition of Radix Glycyrrhizae and clinical research overview；" TCM clinical study ", 2012,4 (16): 31-33 }

Fructus Mume: near for being dried of rose department plant plum Prunus mume (Sieb.) sieb.et Zucc. Mature fruit.Gather during fruit closely maturation in summer, vexed black to complexion changed after oven drying at low temperature.Sour in the mouth, Puckery, property is put down.Return liver, spleen, lung, large intestine channel.Effect is astringed the lung, astringing intestine to stop diarrhea, promotes the production of body fluid." the legendary god of farming Book on Chinese herbal medicine warp " record: " Fructus Mume, the main therapeutic method to keep the adverse QI flowing downwards, heat extraction stuffy sensation with restlessness, feel at ease, only limb pain, hemiplegia Meciless, dead flesh, removes blue or green melanotic nevus, dislikes meat.Energy QI invigorating, the most hungry." Compendium of Material Medica record: " astringing intestine to stop diarrhea of astringing the lung, only chronic cough dysentery, regurgitation is dysphagic, ascariasis of biliary tract vomiting and diarrhea, subsides a swelling and gushes expectorant, parasite killing, Solve ichthyotoxin, horse mump, sulfur poison.”

Fructus Mume main functional component is that organic acid includes citric acid, malic acid etc., possibly together with flavone Class, volatile oil, alkaloid, triterpenes, polysaccharide, sterol etc..In nutritional labeling, alanine High, possibly together with abundant vitamin C, vitamin B1 and B2 with proline content.Modern doctor Learn the effects such as research confirms, Fructus Mume has and protects the liver, antibacterial, antiallergic, allaying tiredness.{ poplar Jade-like stone is luxuriant and rich with fragrance, Hu Hankun, Liu Ping, Liu Wei, Liu Weizhi；Fructus Mume chemical composition, clinical practice and existing It is in progress for pharmacological research；" Chinese pharmacists ", 2012,15 (3): 415-417 }

Pericarpium Citri Reticulatae, also known as Pericarpium Citri tangerinae, for rutaceae orange Citrus reticulata Blanco and cultivation thereof The dry mature skin of training mutation.Medical material is divided into " Pericarpium Citri Reticulatae " and " Pericarpium citri reticuatae chachiensis ".Harvesting ripe Fruit, strips peel, dries or cold drying.Bitter in the mouth, pungent, warm in nature.Return lung, spleen channel. Effect regulating qi-flowing for strengthening spleen, drying dampness to eliminate phlegm.Shennong's Herbal is classified as top grade, records: " tangelo, The main inverse gas of the heat of abdominal mass in the heart, diuretic paddy；Clothes are deodorized for a long time, the therapeutic method to keep the adverse QI flowing downwards, logical god.One Pericarpium Citri tangerinae." " this Grass detailed outline " record: " and Fructus Citri tangerinae skin, treat vomit the sound of vomiting regurgitation noisy, time vomiting watery fluid, fullness and oppression sensation in the epigastrium due to accumulation of phlegm malaria, Large intestine plug, married woman's acute mastitis.Enter foodstuff, solve fish raw meat poison.”

The main functional component of Pericarpium Citri Reticulatae is that flavonoid includes Hesperidin, neohesperidin etc., class Fructus Citri Limoniae Bitter principle includes that limonin, Nomilin etc., volatile oil include α-limonene, beta-myrcene etc., Possibly together with alkaloid, phenolic acids, Coumarins, pectin, sterol, inositol etc..Nutritional labeling In, containing abundant carotenoid.Modern pharmacological research shows, Pericarpium Citri Reticulatae is to gastrointestinal smooth Flesh has the stimulation of gentleness, the secretion of energy facilitating digestion liquid；Meanwhile, also there is anti-inflammation and sterilization, Antioxidation, fatigue-resisting function.{ Ou Lijuan, Liu Qide；Pericarpium Citri Reticulatae Advance on Pharmacological Activities； " China Dispensary ", 2006,17 (10): 787-789 }

Fructus Lycii the kidney invigorating and essence nourishing in Chinese medicine composition side provided by the present invention, nourishing the liver to improve visual acuity, Rhizoma Polygonati nourishing the liver and kidney, spleen invigorating lung moistening；Rhizoma Dioscoreae QI invigorating is reinforced the kidney, strengthening the spleen and nourishing the stomach, and promote the production of body fluid lung benefiting；Chrysanthemum Flower dispelling wind and heat pathogens, liver heat removing and eyesight improving；Radix Glycyrrhizae heat-clearing and toxic substances removing, invigorating the spleen and replenishing QI, hepatoprotective regulating the spleen and stomach, it is in harmonious proportion The property of medicine；Fructus Mume yin fluid astringing tonifying liver, appetizing of promoting the production of body fluid；Pericarpium Citri Reticulatae regulating qi-flowing for strengthening spleen, soothing the liver circulation of qi promoting.Full side is altogether Playing enriching yin and nourishing kidney, the liver benefiting nourishes blood, air making-up and spleen enlivening, and heat clearing away is nourished heart, effect of the lung moistening and production of body fluid promoting.

In order to give Chinese medicine composition various performance, such as, improve mouthfeel, aesthetic property or antioxidation Property, in addition to effective ingredient, the most generally can also comprise one or more desirable Bromatology or pharmaceutically acceptable adjuvant, include but not limited to sweeting agent, antioxidant, acid Taste agent, excipient and preservative, wherein sweeting agent include white sugar, glucose, sugar alcohols, Sucralose, aspartame, acesulfame potassium, stevioside etc., antioxidant includes L-AA (vitamin C), L-AA sodium, arabo-ascorbic acid, sodium erythorbate etc., acidic flavoring agent Including citric acid, malic acid, tartaric acid etc., excipient includes maltodextrin etc., preservative bag Include sorbic acid, potassium sorbate etc..

The Chinese medicine composition of the present invention may be at liquid condition or the solid state being easy to be administered orally, can To make beverage, oral liquid, granule or tablet.Beverage, oral liquid, granule or tablet Can prepare according to procedures known in the art.

According on the other hand, the invention provides the preparation method of above-mentioned Chinese medicine composition.

The preparation method of Chinese medicine composition of the present invention comprises the steps:

(1) in prescription ratio weigh Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae, with the water of 5-20 times of medical material weight, 80-100 DEG C is extracted 2-3 time, each time 0.5-2 Hour；

(2) extracting solution of step (1) is merged, filter, concentrate, obtain concentrated solution 1；

(3) described concentrated solution 1 is filtered, be then centrifuged separating, discard precipitation, obtain The concentrated liquid of tradition Chinese medicine of clarification.

The preparation method of Chinese medicine composition of the present invention preferably includes following steps:

(1) in prescription ratio weigh Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae, extracts 2-3 time at 80-100 DEG C with the water of 5-20 times of medical material weight, each time 0.5-2 hour；

(2) extracting solution in step (1) is merged, filter, concentrate, obtain concentrated solution 1, will Concentrated solution 1 ethanol precipitates, and the supernatant obtained reclaims ethanol, concentrates, obtains concentrated solution 2；

(3) in described concentrated solution 2, add water, carry out water precipitating process, then water precipitating liquid is entered Row centrifugation, discards precipitation, obtains the concentrated liquid of tradition Chinese medicine of clarification.

The preparation method of Chinese medicine composition of the present invention can also preferably include following steps:

(1) in prescription ratio weigh Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae, extracts 2-3 time at 80-100 DEG C with the water of 5-20 times of medical material weight, each time 0.5-2 hour；

(2) being merged by the extracting solution in step (1), be centrifuged processing, supernatant uses film to divide Filter from method, for filtered solution 1；

(3) use reverse osmosis (RO) membrane separation process to concentrate described filtered solution 1, obtain dense Contracting liquid 1, then carries out secondary centrifuging process again, discards precipitation, clarified concentrated solution 1 The concentrated liquid of tradition Chinese medicine.

According to a preferred embodiment, said method farther includes following steps (4): will The concentrated liquid of tradition Chinese medicine of step (3), is spray-dried, obtains traditional Chinese medicinal extract powder.

According to a preferred embodiment, said method farther includes the steps: to The concentrated liquid of tradition Chinese medicine of step (3) or the traditional Chinese medicinal extract powder of step (4), add one or more in accordance with Need bromatology or pharmaceutically acceptable adjuvant, routinely technique make beverage, oral liquid, Granule or tablet.Described adjuvant include but not limited to sweeting agent, antioxidant, acidic flavoring agent, Excipient and preservative.

According to another aspect, the invention provides the health care of above-mentioned composition and pharmaceutical On the way.

The raw material of Chinese medicine composition of the present invention belong to " Ministry of Public Health about further specification health care food The notice of product raw materials control " in (defend method prison send out [2002] No. 51) regulation be food and The article of medicine.Simultaneously the most again through health food Toxicological evaluation it is demonstrated experimentally that this Bright Chinese medicine composition safety, without any side effects.

Chinese medicine composition of the present invention through function of health food check, have alleviating physical fatigue and Asthenopic health care.

Chinese medicine composition of the present invention, through pharmacodynamic study, has improvement memory and sexual function effect.

The method have the benefit that

(1) Chinese medicine composition of the present invention is the raw material of Chinese medicine of integration of edible and medicinal herbs, safe and reliable, nothing Any toxic and side effects, mouthfeel is excellent, cheap and easy to get.

(2) present invention combines with Traditional Chinese Medicine Health preserving theory and modern medicine study, and prescription sets Rationally, compatibility is rigorous for meter, action temperature and progressive, has enriching yin and nourishing kidney, and the liver benefiting nourishes blood, QI invigorating Spleen invigorating, heat clearing away nourishes heart, effect of the lung moistening and production of body fluid promoting, it is possible to effectively eliminate lassitude and weak, persistently transport Muscle fatigue after Dong, the visual fatigue after excess eye-using, concurrently facilitate improvement memory and property Hypofunction.

(3) preparation method of Chinese medicine composition of the present invention is divided with tradition extraction process by water and modern times advanced person Combining from technology, embody the characteristic of tcm prescription, greatly remain in raw material is various Active substance.

Detailed description of the invention

Below in conjunction with embodiment, the present invention is elaborated, but do not constitute the present invention any Limit.

Embodiment 1: the preparation of Chinese medicine composition beverage

1. raw material prepare: weigh Fructus Lycii 26kg, Rhizoma Polygonati 18kg, Rhizoma Dioscoreae 18kg, Flos Chrysanthemi 18kg, Radix Glycyrrhizae 8kg, Fructus Mume 8kg and Pericarpium Citri Reticulatae 4kg.

2. extract and refined: above-mentioned raw materials is joined traditional extraction device or conventional dynamic In extraction element, adding the water of 12 times of medical material weight, 90 DEG C are extracted 1.5 hours, obtain water and carry Liquid 1, medicinal residues add the water of 10 times of medical material weight, and 90 DEG C are extracted 1.0 hours, obtain water and carry Liquid 2, medicinal residues add the water of 8 times of medical material weight, and 90 DEG C are extracted 0.5 hour, obtain water Extract 3, merges Aqueous extracts 1,2 and 3, stands, and medicinal liquid tubular type continuous high speed is centrifugal (centrifugal Machine rotating speed 15000 revs/min), the filtration of supernatant tubular membrane (pump motor frequency 30-50HZ, Permeate flow velocity 2-3 ton hour), filtered solution uses reverse osmosis (RO) membrance concentration (pump motor frequency Rate 30-50HZ, permeate flow velocity 1-2 ton hour), obtain concentrated solution, concentrated solution secondary again Tubular type continuous high speed is centrifuged (centrifuge speed 15000 revs/min), and the airtight filter tank of supernatant filters Degerming, obtain the concentrated liquid of tradition Chinese medicine.

3. allotment and filter: by above-mentioned the concentrated liquid of tradition Chinese medicine, add 6% glucose, 4% white sugar, 0.03%L-ascorbic acid and 0.02% citric acid, be subsequently adding gauge water, filters.

4. fill and sterilization: deployed beverage stock solution, fill after instantaneous ultrahigh-temperature sterilization, i.e. Obtain beverage product.

Embodiment 2: the preparation of Chinese medicine composition oral liquid

1. raw material prepare: weigh Fructus Lycii 1kg, Rhizoma Polygonati 1kg, Rhizoma Dioscoreae 1kg, Flos Chrysanthemi 1kg, Radix Glycyrrhizae 15kg, Fructus Mume 15kg and Pericarpium Citri Reticulatae 8kg.

2. extract and refined: above-mentioned raw materials is joined traditional extraction device or conventional dynamic In extraction element, adding the water of 10 times of medical material weight, 90 DEG C are extracted 1.0 hours, obtain water and carry Liquid 1, medicinal residues add the water of 8 times of medical material weight, and 90 DEG C are extracted 0.5 hour, obtain water and carry Liquid 2, medicinal residues add the water of 5 times of medical material weight, and 90 DEG C are extracted 0.5 hour, obtain water Extract 3, merges Aqueous extracts 1,2 and 3, stands, and medicinal liquid tubular type continuous high speed is centrifugal (centrifugal Machine rotating speed 15000 revs/min), the filtration of supernatant tubular membrane (pump motor frequency 30-50HZ, Permeate flow velocity 2-3 ton hour), filtered solution RO membrance concentration (pump motor frequency 30-50HZ, Permeate flow velocity 1-2 ton hour), obtain concentrated solution, concentrated solution secondary tubular type continuous high speed again Centrifugal (centrifuge speed 15000 revs/min), supernatant airtight filter tank filtration sterilization, in obtaining Medicine concentrated solution.

3. allotment and filtration: by above-mentioned the concentrated liquid of tradition Chinese medicine, add 4% white sugar and 0.03% lemon Lemon acid, is subsequently adding gauge water, filters.

4. fill and sterilization: after deployed oral liquid stock solution fill, water-bath type high temperature sterilize (105 DEG C sterilizing 30 minutes), obtain oral liquid finished product.

Embodiment 3: the preparation of Chinese medicinal composition granules

1. raw material prepare: weigh Fructus Lycii 25kg, Rhizoma Polygonati 15kg, Rhizoma Dioscoreae 15kg, Flos Chrysanthemi 15kg, Radix Glycyrrhizae 0.5kg, Fructus Mume 0.5kg and Pericarpium Citri Reticulatae 0.5kg.

2. extract and refined: above-mentioned raw materials is joined traditional extraction device or conventional dynamic In extraction element, adding the water of 15 times of medical material weight, 95 DEG C are extracted 2.0 hours, obtain water and carry Liquid 1, medicinal residues add the water of 12 times of medical material weight, and 95 DEG C are extracted 1.0 hours, obtain water and carry Liquid 2, medicinal residues add the water of 10 times of medical material weight, and 95 DEG C are extracted 1.0 hours, obtain water Extract 3, merges Aqueous extracts 1,2 and 3, stands, is evaporated to medicinal liquid proportion 1.09-1.13 (70-80 DEG C of survey), after plate-and-frame filtration, medicinal liquid tubular type continuous high speed is centrifuged (centrifugal basket Speed 15000 revs/min), discard precipitation, obtain supernatant, supernatant adds 20% Fructus Hordei Germinatus and sticks with paste Essence, is spray-dried, and collects traditional Chinese medicinal extract powder.

3. granulating and forming and packaging: above-mentioned traditional Chinese medicinal extract powder, adds 40% Icing Sugar, boiling granulating, Sieve, subpackage, obtain granule finished product.

Embodiment 4: the preparation of Chinese medicine composition composition chewable tablet

1. raw material prepare: weigh Fructus Lycii 2kg, Rhizoma Polygonati 15kg, Rhizoma Dioscoreae 15kg, Flos Chrysanthemi 15kg, Radix Glycyrrhizae 2kg, Fructus Mume 2kg and Pericarpium Citri Reticulatae 1kg.

2. extract and refined: above-mentioned raw materials is joined traditional extraction device or conventional dynamic In extraction element, adding the water of 10 times of medical material weight, 95 DEG C are extracted 1.5 hours, obtain water and carry Liquid 1, medicinal residues add the water of 8 times of medical material weight, and 95 DEG C are extracted 1.0 hours, obtain water and carry Liquid 2, merges Aqueous extracts 1 and 2, stands, is evaporated to medicinal liquid proportion 1.10-1.15 (70-80 DEG C Survey), plate-and-frame filtration, concentrated solution adds 95% ethanol to carry out precipitate with ethanol (60%) and processes, and overnight obtains Alcohol supernatant；Above-mentioned alcohol liquid is reclaimed ethanol, is evaporated to medicinal liquid proportion 1.20-1.25 (70-80 DEG C Survey), add 0.5 times amount pure water, be cooled to room temperature, cold preservation (4-10 DEG C) is overnight obtains water precipitating Liquid；Above-mentioned water precipitating liquid tubular type continuous high speed is centrifuged (centrifuge speed 15000 revs/min), abandons Going precipitation, obtain supernatant, supernatant adds 30% maltodextrin, is spray-dried, in collection Medicine extract powder.

3. pelletize: above-mentioned traditional Chinese medicinal extract powder, add 30% Icing Sugar, 10% pregelatinized Starch and 0.1% Aspartame, boiling granulating, sieves, obtains granule.

4. tabletting and subpackage: above-mentioned granule, adds 1.5% micropowder silica gel, tabletting, subpackage, Obtain tablet finished product.

Embodiment 5: Chinese medicine composition chmice acute Oral toxicity is tested

One, detection foundation: Ministry of Public Health " health food inspection and assessment technique specification " (2003 Year version)

Two, material and method

1. given the test agent processes: in embodiment 1, the concentrated liquid of tradition Chinese medicine of step 2 is (the denseest Contracting liquid is 1.) and embodiment 3 in the traditional Chinese medicinal extract powder (hereinafter referred to as extract powder is 3.) point of step 2 Do not add distilled water diluting and be equivalent to 1g raw material of Chinese medicine to 1ml sample.

2. experimental animal: Kunming mouse, male and female half and half, body weight (20 ± 2) g, by Shanghai this Rec laboratory animal company limited provides, production licence number: SCXK (Shanghai) 2012-0002. Receptacle temperature 20 DEG C-25 DEG C, relative humidity 40%-70%.Mouse feed is real by the double lion in Suzhou Test animal feed science service company limited to provide, registration card number: Soviet Union raises careful (2009) 05032.

3. experimental technique:

(1), after animal fasting (can't help water) 16 hours, select mice female, male each by body weight requirement 10, divide and be put in two mouse cages, with the difference of body weight between sex mice less than 3g.

(2) given the test agent uses maximum tolerated dose method contaminate laboratory animal, and mice is by only claiming Weight, gavage capacity press each 0.4ml/20g weighing machine, in 24 hours 2 times to mouse stomach, Gavage interval time of 2 times is 6 hours.

(3), after contamination, observe the general state of animal, body weight change, poisoning symptom and death Situation etc..Observation period is two weeks.

(4) animal is weighed by experiment end again.Dead animal and the animal put to death that expires are carried out Obduction, perusal general pathology changes situation.

(5) experiment overall process and observed content all do itemized record, test by maximum tolerated dose method Result tries to achieve chmice acute per os MTD.

4. experimental result:

(1) main physical signs performance: during test, each treated animal is movable normal, and hair color glossiness is good, Have no any poisoning sign and death；Expire execution animal, each internal organs of gross anatomy perusal Situation, no abnormality seen.

(2) female mice: MTD > 40g raw material of Chinese medicine/kg；Male mice: MTD > 40g Chinese medicine Compositions/kg.

5. experiment conclusion:

Concentrated solution is 1. all resistance to the maximum of chmice acute Oral toxicity female, male test with extract powder It is all higher than 40g raw material of Chinese medicine/kg by dose Zn-MT D, belongs to nontoxic level.

Embodiment 6: Chinese medicine composition function of physical fatigue alleviation is tested

One, detection foundation: Ministry of Public Health " health food inspection and assessment technique specification " (2003 Year version)

Two, material and method

1. experimental animal: Kunming mouse, male, body weight (20 ± 2) g, by Shanghai Si Laike Laboratory animal company limited provides, production licence number: SCXK (Shanghai) 2012-0002.Raise Room temperature 20 DEG C-25 DEG C, relative humidity 40%-70%.Mouse feed is dynamic by Suzhou double lion experiment Thing feed technology Services Co., Ltd provides, registration card number: Soviet Union raises careful (2009) 05032.

2. dose design: the concentrated liquid of tradition Chinese medicine (the hereinafter referred to as concentrated solution of step 2 in embodiment 1 1.) traditional Chinese medicinal extract powder (hereinafter referred to as extract powder is 3.) of step 2, human body and in embodiment 3 Recommended dose is 24g raw material of Chinese medicine/60kg every day, and this experiment sets middle and high two dosage groups, point Not Wei 4g raw material of Chinese medicine/kg, 8g raw material of Chinese medicine/kg, be equivalent to human body recommended dose 10, 20 times, separately set distilled water blank group.

3. given the test agent processes: 1. concentrated solution adds distilled water diluting the most respectively to 1ml with extract powder Sample is equivalent to 0.2g raw material of Chinese medicine, and 1ml sample is equivalent to 0.4g raw material of Chinese medicine, in being, The test liquid of high two dosage groups.

4. route of administration: gavage, gavage capacity 0.4ml/20g body weight.

5. statistical method: experimental result all carries out variance analysis with SPSS13.0 version software.

6. experimental technique and result

Take mice 150, by only weighing, be divided into 15 groups by body weight stratified random, often organize 10 Only.It is one with every 5 groups and tests big group, conduct an experiment.All animals are according to dosage designed Feed 30 days continuously, weigh weekly, adjust to sample amount according to body weight.

6.1 Loaned swimming tests:

After last gavage 30 minutes, mice is put into water went swimming, depth of water 30cm, water temperature 25 DEG C ± 1 DEG C, mouse tail root load 5% body weight sheet lead, mice starts till death record from swimming The time died.The results are shown in Table 1.

The impact on the mice burden swimming time of table 1 sample

Note: * P < 0.05, compares with matched group

As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference； Concentrated solution 1. with extract powder 3. high dose group compared with matched group, walking weight load is obviously prolonged, There is significant difference.

6.2 serum ureas measure:

After last gavage 30 minutes, mice is put into not swimming with a load attached to the body 90 minutes in 30 DEG C of water, Taking-up is dried, and takes a blood sample after having a rest 60 minutes, centrifugal, takes serum and surveys carbamide.The results are shown in Table 2.

Table 2 sample is on the impact of serum urea after mouse movement

Note: * P < 0.05, compares with matched group

As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference； Concentrated solution 1. with extract powder 3. high dose group compared with matched group, serum urea level significantly reduces, There is significant difference.

6.3 hepatic glycogen measure

Within 30 minutes after last gavage, put to death mice, take liver at once, float in normal saline Wash rear wipe dry, detect hepatic glycogen.The results are shown in Table 3.

The impact on Mouse Liver glycogen of table 3 sample

Note: * P < 0.05, compares with matched group

As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference； Concentrated solution 1. with extract powder 3. high dose group compared with matched group, hepatic glycogen level substantially increases, There is significant difference.

7. conclusion: under this experimental condition, Loaned swimming test result is positive, and serum urine Element, hepatic glycogen index are positive, therefore think that 3. 1. concentrated solution have alleviation body with extract powder to mice Power fatigue function.

Embodiment 7: Chinese medicine composition alleviates asthenopia functional experiment

One, detection foundation: " about printing and distributing 9 health cares such as anti-oxidation function evaluation methodology The notice of evaluation methodology " (state food medicine prison guarantorization [2012] 107) adnexa 4 alleviates asthenopia function Evaluation methodology.

Two, experimenter's inclusive criteria: by the principle of voluntariness select the age be 18-65 year, use for a long time Eye, the fatigable experimenter of vision 120.

Three, Subject Exclusion Criteria: suffer from ocular disease person infectious, traumatic；Carry out eye Portion performs the operation less than 3 months persons；Suffer from external eyes in cornea, crystal, vitreous body, retinopathy etc. Disease patient；Suffer from the disease patients such as cardiovascular, cerebrovascular, liver, kidney, hemopoietic system；Gestation or Women breast-feeding their children and allergic constitution patient；Take the article relevant with tested function, shadow in a short time Ring the judgement person to result；Long-term taking is about the treatment medicine of vision, health food or use Other treatment method fails terminator；Do not meet inclusive criteria, not by the edible tested material person of regulation Or data is congruent affects effect or safety judgement person.

Four, test method

1. test packet: 120 example experimenters use random double-blind method to be divided into test-meal group and placebo Matched group, often organizes each 60 examples, and the factor such as age, sex, vision has comparability, test Use and compare between self cross-reference and group.

2. test-meal method: test-meal group takes beverage in embodiment 1 (hereinafter referred to as beverage is 1.), Matched group takes placebo, is every day 2 times, each 1 bottle, and Time of Administration is 60 days.

3. observation index: indices in test-meal on-test and at the end of each measure 1 time.

3.1 safety indexes: general physical examination blood, urine, feces are conventional, biochemical indicator of having a blood test, Chest X-rays, electrocardiogram, ultrasound diagnosis, untoward reaction is observed.

3.2 efficiency indexes: in test-meal start and at the end of check.

3.2.1 questionnaire survey: symptom is inquired, used eye situation.

3.2.2 ophthalmologic examination: include examination of ocular fundus, examination of visual acuity (myopia, hypermetropia, astigmatism etc.).

3.2.3 duration of photopic vision: it is lasting that the photopic vision time is referred to as photopic vision to the percentage ratio of fixation time Degree.

3.3 effect criterion

3.3.1 duration of photopic vision: test-meal group self compares or compares between test-meal group with matched group group, Duration of photopic vision difference has significance (p < 0.05), and average duration of photopic vision raising is more than or equal to 10% is effective.

3.3.2 symptom average integral: calculate the symptom integral before and after every examination trencherman's test-meal, point Do not calculate the average product score value of two groups, and carry out statistical test.

Table 4 asthenopia symptom decision method (sxemiquantitative integration method)

Note: " random thoughts " refers to 1-2 time/2 days；" time have " refers to 1-3 times/day；" warp Often " refer to 3 times/day

4. statistical method: data statistics is all with SPSS13.0 version software kit, enumeration data employing Chi-square criterion, measurement data uses t inspection.

Five, result of the test

1. the physical data before subject: observe 120 example asthenopes altogether, be randomly divided into Each 60 examples of test-meal group, matched group.At the end of test-meal, test-meal group 7 example is checked the most on time, has 3 Example is dropped by the wayside；Matched group has 8 examples to check the most on time, and 2 examples are dropped by the wayside.Test-meal group is with right Being respectively arranged with 10 examples according to group and meet Subject Exclusion Criteria, case depigmentation rate is 16.67%.Meet Test-meal group 50 example of inclusive criteria, wherein male 12 example, women 38 example.Matched group 50 example, Wherein male 11 example, women 39 example.All experimenter is normal through interior eye, examination of external eye. Two groups of indices no significant differences (P > 0.05) before test-meal.Specifically it is shown in Table 5.

Before table 5 test-meal, physical data compares

Group	Effectively number of cases	Age (year)	Duration of photopic vision (%)	Symptom integral
					Test-meal group	50	46.50±6.79	56.59±4.59	10.22±2.33
Matched group	50	48.32±7.22	57.32±4.48	10.24±2.35

2. duration of photopic vision change: be specifically shown in Table 6

Duration of photopic vision change statistics before and after table 6 test-meal

Group	Effectively number of cases	Before test-meal (%)	After test-meal (%)	Improve (%)
					Test-meal group	50	56.59±4.59	66.96±4.91##**	10.37±3.54**
Matched group	50	57.32±4.48	58.84±4.81	1.52±1.98

Note: ## compares P < 0.01 with self comparing P < 0.01, * * before test-meal with matched group.

3. symptom integral change: be specifically shown in Table 7.

Symptom integral statistics (± SD) before and after table 7 test-meal

Group	Effectively number of cases	Before test-meal	After test-meal	Difference
					Test-meal group	50	10.46±2.42	7.20±2.11##**	3.26±1.63**
Matched group	50	10.28±2.27	9.56±1.80	0.72±1.11

Note: ## compares P < 0.01 with self comparing P < 0.01, * * before test-meal with matched group.

Six. conclusion:

Under this is studied, test-meal group duration of photopic vision, before and after test-meal, self comparing difference has aobvious Write property (P < 0.01), after test-meal duration of photopic vision improve 10.37%, more than 10%, and with compare After group test-meal, duration of photopic vision comparing difference has significance (P < 0.01)；Test-meal group symptom integral, Before and after test-meal, self comparing difference has significance (P < 0.01), symptom integral deduction value after test-meal, Significance (P < 0.01) is had with matched group symptom integral deduction value comparing difference.Therefore think that beverage is 1. Human body is had alleviation asthenopia effect.

Embodiment 8: Chinese medicine composition auxiliary improving memory function is tested

One, detection foundation: Ministry of Public Health " health food inspection and assessment technique specification " (2003 Year version)

Two, material and method

1. experimental animal: Kunming mouse, male, body weight (20 ± 2) g, by Shanghai Si Laike Laboratory animal company limited provides, production licence number: SCXK (Shanghai) 2012-0002.Raise Room temperature 20 DEG C-25 DEG C, relative humidity 40%-70%.Mouse feed is dynamic by Suzhou double lion experiment Thing feed technology Services Co., Ltd provides, registration card number: Soviet Union raises careful (2009) 05032.

2. dose design: the concentrated liquid of tradition Chinese medicine (the hereinafter referred to as concentrated solution of step 2 in embodiment 1 1.) traditional Chinese medicinal extract powder (hereinafter referred to as extract powder is 3.) of step 2, human body and in embodiment 3 Recommended dose is 24g raw material of Chinese medicine/60kg every day, and this experiment sets middle and high two dosage groups, point Not Wei 4g raw material of Chinese medicine/kg, 12g raw material of Chinese medicine/kg, be equivalent to human body recommended dose 10, 30 times, separately set distilled water blank group.

3. given the test agent processes: 1. concentrated solution adds distilled water diluting the most respectively to 1ml with extract powder Sample is equivalent to 0.2g raw material of Chinese medicine, and 1ml sample is equivalent to 0.6g raw material of Chinese medicine, in being, The test liquid of high two dosage groups.

4. route of administration: gavage, gavage capacity 0.4ml/20g body weight.

5. statistical method: experimental result all carries out variance analysis with SPSS13.0 version software.

6. experimental technique and result

Take mice 100, by only weighing, be divided into 10 groups by body weight stratified random, often organize 10 Only.It is one with every 5 groups and tests big group, conduct an experiment.All animals are according to dosage designed Feed 45 days continuously, weigh weekly, adjust to sample amount according to body weight.

6.1 Jumping test:

After last gavage, next day starts training.Mice is put into reaction chamber endoadaptation 3 minutes, so After by the copper grid in mice placing response case, pass to 36 volts of alternating currents immediately, mice is just Paradoxical reaction is to jump back on insulated platform.Most animals may skip on copper grid again or repeatedly, Platform is snapped back again by electric shock.After training once, the platform that animal is placed in reaction chamber On, in recording 5 minutes, each Mus jumps off the errors number of platform and jumps off hiding of platform for the first time Phase, in this, as school grade.The same time after 24h, carry out cyclical test, mice is put On platform, record each Mus and jump off for the first time the incubation period of platform, each Mus and shock by electricity secondary in 3 minutes Number and the number of animals sum shocked by electricity, calculate the percentage rate of animal wrong reaction occur simultaneously (number of animals shocked by electricity accounts for the percentage rate that this treated animal is total).The results are shown in Table 8.

The impact on mice Jumping test of table 8 sample

Note: * P < 0.05, compares with matched group

As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference； Concentrated solution 1. with extract powder 3. high dose group compared with matched group, the incubation period jumping off platform is obvious Extend, have significant difference.

6.2 step-through test:

After last gavage, next day starts training.During experiment, bright room is put at mice face hole dorsad, Starting timer, mice is shocked by electricity through entrance darkroom, hole simultaneously, and timer stops, and takes Go out mice, record every Mus and suffer the required time of shocking by electricity to entrance darkroom from putting into bright room, the latentest Fu Qi, trains 5 minutes, and records number of shocks in 5 minutes.After 24h, the same time, enters Row cyclical test, records every Mus and enters incubation period and the 5 minutes interior number of shocks in darkroom, and The animal percentage rate in darkroom (wrong reaction) is entered in calculating 5 minutes.The results are shown in Table 9.

The impact on mice step-through test of table 9 sample

Note: * P < 0.05, compares with matched group

As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference； Concentrated solution 1. with extract powder 3. high dose group compared with matched group, enter darkroom incubation period obvious Extend, have significant difference.

7. conclusion: under this experimental condition, Jumping test and step-through test result are positive, therefore Think that 3. 1. concentrated solution have auxiliary improving memory function to mice with extract powder.

Embodiment 9: Chinese medicine composition is to castrated rats penile erectile function and reproductive development Impact

One, experimental basis: Qi Chen edits " herbal pharmacology research methodology "

Two, material and method

1. experimental animal: Wistar rat, male, body weight 100g, Shanghai Si Laike test Company of Animals Ltd. provides, production licence number: SCXK(Shanghai) 2012-0002.Receptacle Temperature 20 DEG C-25 DEG C, relative humidity 40%-70%.Rat feed is by the double lion laboratory animal in Suzhou Feed technology Services Co., Ltd provides, registration card number: Soviet Union raises careful (2009) 05032.

2. the foundation of model: 50 male rats, uses ether light anaesthesia, routine disinfection, cuts Except both sides testis.In second day after operation random packet.

3. dose design: the concentrated liquid of tradition Chinese medicine (the hereinafter referred to as concentrated solution of step 2 in embodiment 1 1.) traditional Chinese medicinal extract powder (hereinafter referred to as extract powder is 3.) of step 2, human body and in embodiment 3 Recommended dose is 24g raw material of Chinese medicine/60kg every day, and this experiment sets middle and high two dosage groups, point Not Wei 2g raw material of Chinese medicine/kg, 6g raw material of Chinese medicine/kg, be equivalent to the 5 of human body recommended dose, 15 Times, separately set distilled water blank group.

4. given the test agent processes: 1. concentrated solution adds distilled water diluting the most respectively to 1ml with extract powder Sample is equivalent to 0.4g raw material of Chinese medicine, and 1ml sample is equivalent to 1.2g raw material of Chinese medicine, in being, The test liquid of high two dosage groups.

5. route of administration: gavage, gavage capacity 0.5mL/100g body weight.

6. statistical method: experimental result all carries out variance analysis with SPSS13.0 version software.

7. experimental technique and result

Take rat 50, by only weighing, be divided into 5 groups by body weight stratified random, often group 10. All animals are according to dosage designed and feed 45 days continuously, weigh weekly, adjust to sample according to body weight Amount.1h after last gavage, is positioned over the stimulating electrode of electrostimulator rat penis position, gives Give surface element electricity irritation, record from stimulating beginning to the erection time (erection incubation period)； Again rat put to death and dissect each group of rat, weighing preputial glands, seminal vesicle+and prostate immediately Weight, calculate organ index.The results are shown in Table 10.

Table 10 sample is on castrated rats penile erectile function and the impact of reproductive development

Note: * P < 0.05, compares with matched group

As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference； Concentrated solution 1. with extract powder 3. high dose group compared with matched group, can substantially shorten castrated rats cloudy Stem erects incubation period, can be obviously promoted castrated rats preputial glands, prostate and seminal fluid capsule simultaneously Growth promoter, has significant difference.

8. conclusion: under this experimental condition, the experiment of castrated rats penile erectile function and reproduction The result of allelotaxis's experiment is positive, therefore thinks that 3. 1. concentrated solution can assist improvement to go with extract powder Gesture sexual function of rats.

It is last it should be understood that above-described embodiment is only to be made by the clearly explanation present invention Citing, and not restriction to embodiment.Those of ordinary skill in the field are come Say, change or the variation of other multi-forms can also be made on the basis of the above description.This In without also cannot all of embodiment be given exhaustive.And thus amplified out aobvious and easy The change seen or variation still in protection scope of the present invention among.

Claims (8)

1. a Chinese medicine composition is used for alleviating in asthenopic health product or medicine in preparation Purposes, described Chinese medicine composition active component by Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae composition, weight proportion is: Fructus Lycii 13 parts, Rhizoma Polygonati 9 parts, Rhizoma Dioscoreae 9 parts, Flos Chrysanthemi 9 parts, 4 parts of Radix Glycyrrhizae, Fructus Mume 4 parts and Pericarpium Citri Reticulatae 2 parts.

2. Chinese medicine composition as claimed in claim 1 is used for alleviating asthenopic guarantor in preparation Purposes in strong product or medicine, it is characterised in that described Chinese medicine composition also comprises one or many Plant bromatology or pharmaceutically acceptable adjuvant.

3. Chinese medicine composition as claimed in claim 1 is used for alleviating asthenopic guarantor in preparation Purposes in strong product or medicine, it is characterised in that the preparation method of described Chinese medicine composition includes step Rapid:

(1) weight proportion in the Chinese medicine composition as described in claim 1 weigh Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae, with the water of 5-20 times of medical material weight, 80-100 DEG C is extracted 2-3 time, 0.5-2 hour each time；

(2) extracting solution of step (1) is merged, filter, concentrate, obtain concentrated solution 1；

(3) described concentrated solution 1 is filtered, be then centrifuged separating, discard precipitation, obtain The concentrated liquid of tradition Chinese medicine of clarification.

4. Chinese medicine composition as claimed in claim 1 is used for alleviating asthenopic guarantor in preparation Purposes in strong product or medicine, it is characterised in that the preparation method of described Chinese medicine composition includes step Rapid:

(1) weight proportion in the Chinese medicine composition as described in claim 1 weigh Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae, with the water of 5-20 times of medical material weight Extract 2-3 time at 80-100 DEG C, 0.5-2 hour each time；

(2) extracting solution in step (1) is merged, filter, concentrate, obtain concentrated solution 1, will Concentrated solution 1 ethanol precipitates, and the supernatant obtained reclaims ethanol, concentrates, obtains concentrated solution 2；

(3) in described concentrated solution 2, add water, carry out water precipitating process, then water precipitating liquid is entered Row centrifugation, discards precipitation, obtains the concentrated liquid of tradition Chinese medicine of clarification.

5. Chinese medicine composition as claimed in claim 1 is used for alleviating asthenopic guarantor in preparation Purposes in strong product or medicine, it is characterised in that the preparation method of described Chinese medicine composition includes step Rapid:

(1) weight proportion in the Chinese medicine composition as described in claim 1 weigh Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae, with the water of 5-20 times of medical material weight Extract 2-3 time at 80-100 DEG C, 0.5-2 hour each time；

(2) being merged by the extracting solution in step (1), be centrifuged processing, supernatant uses film to divide Filter from method, for filtered solution 1；

(3) use reverse osmosis (RO) membrane separation process to concentrate described filtered solution 1, obtain dense Contracting liquid 1, then carries out secondary centrifuging process again, discards precipitation, clarified concentrated solution 1 The concentrated liquid of tradition Chinese medicine.

6. as described in claim 3-5 any one, Chinese medicine composition regards tired in preparation for alleviation Purposes in the health product of labor or medicine, the preparation method of described Chinese medicine composition farther includes Following steps (4): by the concentrated liquid of tradition Chinese medicine of step (3), are spray-dried, obtain traditional Chinese medicinal extract powder.

7. as described in claim 3-5 any one, Chinese medicine composition regards tired in preparation for alleviation Purposes in the health product of labor or medicine, the preparation method of described Chinese medicine composition farther includes Following steps: to the concentrated liquid of tradition Chinese medicine of step (3), add one or more bromatologies or pharmaceutically Acceptable adjuvant, technique makes beverage, oral liquid, granule or tablet routinely.

8. Chinese medicine composition is used for alleviating asthenopic health care in preparation as claimed in claim 6 Purposes in product or medicine, the preparation method of described Chinese medicine composition farther includes to walk as follows Rapid: to the traditional Chinese medicinal extract powder of step (4), add one or more bromatologies or pharmaceutically acceptable Adjuvant, technique makes beverage, oral liquid, granule or tablet routinely.