CN103893549A - Traditional Chinese medicine composition for relieving fatigue as well as preparation method and application of composition - Google Patents
Traditional Chinese medicine composition for relieving fatigue as well as preparation method and application of composition Download PDFInfo
- Publication number
- CN103893549A CN103893549A CN201410160138.8A CN201410160138A CN103893549A CN 103893549 A CN103893549 A CN 103893549A CN 201410160138 A CN201410160138 A CN 201410160138A CN 103893549 A CN103893549 A CN 103893549A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- parts
- medicine composition
- concentrated
- pericarpium citri
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a traditional Chinese medicine composition for relieving fatigue as well as a preparation method and application of the composition. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 1-50 parts of fruit of Chinese wolfberry, 1-30 parts of rhizoma polygonati, 1-30 parts of Chinese yam, 1-30 parts of chrysanthemum, 1-15 parts of liquorice, 1-15 parts of smoked plum and 1-8 parts of pericarpium citri reticulatae. The preparation method disclosed by the invention comprises the following steps: adopting a water extracting process; applying the technologies such as centrifugation and membrane separation, so as to obtain traditional Chinese medicine concentrated liquor; or carrying out spray drying to obtain traditional Chinese medicine extract powder; adding auxiliary materials to prepare beverage, oral liquid, granula or tablets. The traditional Chinese medicine composition disclosed by the invention has the effects of relieving physical fatigue and visual fatigue, and helping improvement of memory and sexual function, and is accurate in effect, and safe and free of toxic and side effects.
Description
Technical field
The invention belongs to medical technical field, be specifically related to a kind of alleviating physical fatigue and asthenopia, contribute to Chinese medicine composition improving memory and sexual function and its production and use.
Background technology
Along with the quick transition of society, in communication society, the video display terminals such as computer, television set, mobile phone are penetrated into the every nook and cranny of daily life, add the quickening of work and rhythm of life, increasing people is in sub-health state, cardinal symptom shows as fatigue, comprises physical fatigue, mental fatigue and asthenopia etc., affects quality of life.
Modern medicine thinks, fatigue is that local organization, the organ dysfunction due to many reasons goes down or the subjective sensation of general malaise, shows as weakening of work capacity in behavioristics.In existing document, tired classification is mainly contained following several: be divided into physical fatigue and mental fatigue according to the reason producing; Be divided into acute fatigue and confirmed fatigue according to the persistent period; Be divided into whole body fatigue and local fatigue according to producing position.Physical fatigue claims again physiological fatigue, muscle fatigue or physical fatigue.To the search time of physiological fatigue early, the sports fatigue that begin one's study the eighties in 19th century just refers to physical fatigue to modern medicine.The biochemical meeting of the 5th international movement that nineteen eighty-two holds in Boston is defined as sports fatigue: organism physiology process can not continue its function on specified level and (or) can not maintain predetermined exercise intensity.Mental fatigue claims again Subjective fatigue, mental fatigue or psychological fatigue." psychology dictionary " pointed out explaining when mental fatigue: psychological fatigue refers to that the cognition that spiritual undue concentration or puzzlement cause is tired.Mental fatigue main manifestations is difficult concentrating, thinking difficulty, and Vigilance reduces, hypomnesis, hyposexuality etc.Asthenopia one word is derived by Hellenic expression method, and original text means " weak eye " (eye is unable).Existing most ophthalmology scholars think primary disease be symptom take the conscious eye of patient as basis, the syndrome that eye or whole body apparatus factors and spirit (psychology) factor are interweaved.Thereby be also often called as eyestrain's syndrome.The nutrient substance of fatigue alleviating mainly contains: saccharide, lipid, protein, power and water solution matter, trace element, antioxidant comprises superoxide dismutase, catalase, acetone acid etc., aminoacid comprises leucine, tyrosine, aspartic acid, glutamine, taurine, creatine etc., and vitamin comprises that vitamin C, vitamin E, L-carnitine, vitamin B group are as B1, B2, B6, B12 etc.; The chemical substance of resisting fatigue is mainly caffeine etc.On market with the relevant health product of nutrient substance and chemical substance compatibility mainly contain red cattle, force health-care, day fill it up with, Qi Li, happy tiger etc.
Classical Chinese medicine Huangdi's Internal Classics has discussion to fatigue, is called " tired ", " Duo ", " separating lazy ", " tired thin ", " heavy sensation of the body ", " body weight " etc. more.A tired word is first appeared in Chinese Zhang Zhongjing " Medical Treasures of the Golden Chamber ": " ask and say: the teacher what arthralgia due to stagnation of blood obtain from says: husband's rank and honour people weak bone skin is contained, heavy because of tired sweating, shaking frequently when lying in bed, adds by mild wind, then it." Tang's Sun Simiao " prescriptions worth thousand gold ": " and five kinds of over strain person: says will labor, two say think of labor, and three say the impairment of the heart caused by overstrain, and four say care-laden and overworked, and five say fatigue ... its reading, game etc. excessively use order person, says hepatic asthenia." Chinese medicine thinks, it is the smart qi-blood-body fluid in body that muscle power produces the material base maintaining with endurance, the change of these materials is raw closely related with defeated cloth and function of five internal organs; Physical fatigue and liver spleen are in close relations, and asthenopia and Liver and kidney are in close relations, and mental fatigue and heart spleen are in close relations.Traditional Chinese medical science allaying tiredness main principle is tonification and conditioning, and tonification mainly comprises YIN nourishing and YANG invigorating, QI invigorating with enrich blood, nourishing the liver and kidney etc.; Conditioning mainly comprises that spleen invigorating invigorates blood circulation, depressed liver-energy dispersing and QI regulating etc.On market, the relevant health product of Chinese medicine class are mainly take YANG invigorating Chinese medicines such as Cornu Cervi Pantotrichum, Lac regis apis, Radix Ginseng and Radix Panacis Quinquefoliis as main.
Therefore, on the one hand, although the existing product for fatigue alleviating, improvement memory and sexual function is of a great variety, but the various shortcomings of these product ubiquities, the untoward reaction such as be overexcited as long-term taking easily causes, hormonal readiness is unbalance, the resultant effect for the treatment of conditioning is limited and expensive etc.On the other hand, at present for simultaneously alleviating physical fatigue and asthenopia, and there is demand in the tcm product that improves memory and sexual function and safe without toxic side effect.
Summary of the invention
In view of this, the invention provides the Chinese medicine composition and preparation method thereof of the progressive fatigue alleviating of a kind of gentleness; In addition, the present invention also provides the purposes of this Chinese medicine composition.
In order to realize foregoing invention object, according to one side, the invention provides the Chinese medicine composition of fatigue alleviating, its active component by following weight portion forms: Fructus Lycii 1-50 part, Rhizoma Polygonati 1-30 part, Rhizoma Dioscoreae 1-30 part, Flos Chrysanthemi 1-30 part, Radix Glycyrrhizae 1-15 part, Fructus Mume 1-15 part and Pericarpium Citri Reticulatae 1-8 part.
According to a preferred embodiment, described weight proportion is: Fructus Lycii 2-25 part, Rhizoma Polygonati 2-15 part, Rhizoma Dioscoreae 2-15 part, Flos Chrysanthemi 2-15 part, Radix Glycyrrhizae 2-9 part, Fructus Mume 2-9 part and Pericarpium Citri Reticulatae 1-5 part.According to another preferred embodiment, described weight proportion is: 2 parts of 13 parts of Fructus Lycii, 9 parts of Rhizoma Polygonatis, 9 parts of Rhizoma Dioscoreaes, 9 parts of Flos Chrysanthemis, 4 parts, Radix Glycyrrhizae, 4 parts of Fructus Mumes and Pericarpium Citri Reticulataes.
In above-mentioned prescription, source, nature and flavor to each raw material, return through, effect and relevant modern study and be described below:
Fructus Lycii: be the dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L..Summer, autumn gather when two seasons, fruit took on a red color, and hot-air seasoning, removes carpopodium, or dry in the air to rhicnosis, dry, and remove carpopodium.Sweet in the mouth, property is flat.Return liver, kidney channel.Effect nourishing the liver and kidney, replenishing vital essence to improve eyesight." Shennong's Herbal classifies as top gradely, record: " main five interior pathogens, hanker quenching one's thirst; General arthralgia, takes hard muscles and bones for a long time, makes light of one's life by commiting suicide not old." Compendium of Material Medica record: " mend vital essence various symptoms and signs of deficiency, easily color, bleaches, and improving eyesight is calmed the nerves, and makes us long-lived.”
Fructus Lycii major function composition is betanin, flavonoid, polysaccharide etc., and in nutritional labeling, proline and arginine content are high, and carotenoid content is high, also contains vitamin C precursor 2-O-β-D-Glucose base-L-AA (AA-2 β G).Modern medicine proves, Fructus Lycii has antioxidation, defying age, and prevention macula lutea sexually transmitted disease (STD) becomes, and strengthens hemopoietic function, the multiple biological activity such as resisting fatigue.{ in grand; The chemical composition of Fructus Lycii and biological activity; " external medical plant amedica fascicle ", 2007,22 (2): 51-53 }
Rhizoma Polygonati: be the dry rhizome of liliaceous plant Yunnan Rhizoma Polygonati Polygonatum kingianum Coll.Et Hemsl., Rhizoma Polygonati Polygonatum sibiricumRed. or Polygonatum cyrtonema Hua Polygonatum cyrtonema Hua.By shape difference, practise and claim " large Rhizoma Polygonati ", " polygonatum sibiricum Redoute ", " RHIZOMA POLYGONATI ZINGIBERIFORME ".Two seasons of spring and autumn excavate, and remove fibrous root, clean, and put in boiling water and slightly scald or the steaming heart extremely thoroughly, dry.Sweet in the mouth, property is flat.Return spleen, lung, kidney channel.Effect boosting qi and nourishing yin, spleen invigorating, lung moistening, kidney tonifying.Compendium of Material Medica is recorded: " mend all void, only cold and heat, replenishing essence marrow.”
Rhizoma Polygonati major function composition is that steroid saponin comprises diosgenin, Sarsasapogenin etc., also contains polysaccharide, alkaloid, ketone, phlegmatic temperament etc.In nutritional labeling, arginine and threonine content are high.Modern study shows, Rhizoma Polygonati has resisting fatigue, improves and improve memory, antioxidation, blood sugar lowering, the effects such as blood fat reducing.{ Dong Zhicheng, thanks to Zhaoming, Plumbum preparatium, Li Shunxiang; Rhizoma Polygonati resource, chemical composition and pharmacological research overview; " Central-South pharmacy ", 2012,10 (6): 450-453 }
Rhizoma Dioscoreae: be the dry rhizome of Dioscoreaceae plant Rhizoma Dioscoreae Dioscorea opposita Thunb..Winter, stem and leaf was excavated after withering, and cut root head, cleaned, and removed crust and fibrous root, dry, or took advantage of fresh-cut sheet, dry; Also have and select loose straight dry Rhizoma Dioscoreae, put in clear water, be dipped into without the dry heart, vexed, trim two ends, be twisted into cylindricly with plank, dry polishing, habit title " light yam ".Sweet in the mouth, property is flat.Return spleen, lung, kidney channel.Effect spleen reinforcing nourishing the stomach, the lung benefiting that promotes the production of body fluid, the kidney invigorating arresting seminal emission.Shennong's Herbal: classify as top gradely, record: " tonify deficiency is thin for Rhizoma Dioscoreae, main damaging the spleen and stomach, except cold and heat pathogen.Invigorating middle warmer, physical strength profiting, longue meat.Take for a long time normal hearing and eyesight, make light of one's life by commiting suicide, not hungry, prolong life." Compendium of Material Medica record: " kidney tonifying gas, strengthening the spleen and stomach, antidiarrheal dysentery, the saliva that reduces phlegm, profit fur.”
Rhizoma Dioscoreae major function composition is steroid saponin, polysaccharide etc., also contains allantoin, phlegmatic temperament, sterol, adenosine, choline etc.In nutritional labeling, glutamic acid, aspartic acid and arginine content are high, contain and enrich vitamin C, vitamin B1 and B2.Modern pharmacological research shows, Rhizoma Dioscoreae has enhancing intestinal absorption ability, and antioxidation regulates immunity, blood sugar lowering, the pharmacologically actives such as blood fat reducing.{ Yang Xiu shrimp; Rhizoma Dioscoreae chemical composition and pharmacology activity research progress; " Asia-Pacific traditional medicine ", 2013,9 (5): 65-66 }
Flos Chrysanthemi: be feverfew chrysanthemum Chrysanthemum morifolium Ramat. dry capitulum.Spend the 9-11 month while blooming and gather in batches, dry in the shade or dry, or dry after smoking, steaming.Medical material is different with processing method by the place of production, is divided into " Flos Chrysanthemi ", " Flos Chrysanthemi ", " Flos Chrysanthemi ", " Flos Chrysanthemi ".Sweet in the mouth, hardship, cold nature.Return lung, Liver Channel.Effect wind heat clearing away of faling apart, suppressing the hyperactive liver improving eyesight, heat-clearing and toxic substances removing.Shennong's Herbal is classified as top grade, records: " main all wind, dizziness, swells and ache, and order wish is de-, lacrimation; The dead flesh of skin, aversion to wind arthralgia chiefly caused by damp pathogen.Take for a long time sharp vim and vigour, make light of one's life by commiting suicide resistance to old, prolong life." Compendium of Material Medica records: " and chrysanthemum Chun Shengxia is luxuriant, and Qiu Huadong is real, enjoys four gas, experiences all kinds of and reveals frost, and leaf is withered not to fall, spend withered not zero, the taste sweetness and bitterness of holding concurrently, property is reported gentle.In cup, body is light, and angle eats also.”
Flos Chrysanthemi major function composition is that flavonoid comprises luteoloside, Flos Buddlejae glycosides etc., and phenolic acids comprises chlorogenic acid, 3,5-O-dicaffeoyl-quinic acid etc., and volatile oil comprises Camphora, Borneolum Syntheticum, beta-elemene etc., also contains echinacoside, polysaccharide etc.In nutritional labeling, proline and content of glutamic acid are high.Chinese scholars research shows, Flos Chrysanthemi has antioxidation, anti-bacteria and anti-virus, the multiple pharmacologically active such as treatment coronary heart disease, reduces blood pressure, prevention hyperlipidemia.{ Jiang Baoping, Xu Lijia, Wang Qiuling, Xiao Peigen; The tradition use of Flos Chrysanthemi and chemical composition and pharmacology activity research progress; " contemporary Chinese Chinese medicine ", 2013,15 (6): 523-530 }
Radix Glycyrrhizae: be dry root and the rhizome of glycyrrhizic legume Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. Glycyrrhiza glabra L..Spring, Qiu Erji excavate, and remove fibrous root, dry.Sweet in the mouth, property is flat.GUIXIN, lung, spleen, stomach warp.Effect invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.Shennong's Herbal is classified as top grade, records: " main the five internal organs six mansion cold and heat pathogens; Hard muscles and bones, longue meat, times power; Incised wound is swollen; Removing toxic substances.Clothes are made light of one's life by commiting suicide and are prolonged life for a long time." Compendium of Material Medica record: " separate infantile carbuncle infantile convulsion, pathogenic fire reducing pain relieving.”
Radix Glycyrrhizae major function composition is that saponins comprises glycyrrhizin, glycyrrhizic acid, enoxolone etc., and flavonoid comprises liquirtin, the different glycosides of Radix Glycyrrhizae etc., also contains Coumarins, alkaloid, polysaccharide etc.In nutritional labeling, proline and arginine content are high.Modern clinical research confirmation, Radix Glycyrrhizae has hepatoprotective, antitumor, anti-bacteria and anti-virus, antiulcer, the clinical effects such as pain relieving.{ field actor playing a martial role in Chinese operas; The chemical composition of Radix Glycyrrhizae and clinical research overview; " tcm clinical practice research ", 2012,4 (16): 31-33 }
Fructus Mume: be the dry almost ripe fruit of rose department plant plum Prunus mume (Sieb.) sieb.et Zucc..When the nearly maturation of fruit in summer, gather, vexed black to complexion changed after oven drying at low temperature.Sour in the mouth, puckery, property is flat.Return liver, spleen, lung, large intestine channel.Effect is astringed the lung, and astringing intestine to stop diarrhea promotes the production of body fluid.Shennong's Herbal is recorded: " heat extraction stuffy sensation with restlessness, feels at ease for Fructus Mume, the main therapeutic method to keep the adverse QI flowing downwards, and only limb pain, hemiplegia meciless, and dead flesh, removes blue or green melanotic nevus, dislikes meat.Energy QI invigorating, not hungry." Compendium of Material Medica record: " and regurgitation is dysphagic for the astringing intestine to stop diarrhea of astringing the lung, only chronic cough dysentery, ascariasis of biliary tract vomiting and diarrhea, and expectorant is gushed in detumescence, and parasite killing is separated ichthyotoxin, horse mump, sulfur poison.”
Fructus Mume major function composition is that organic acid comprises citric acid, malic acid etc., also contains flavonoid, volatile oil, alkaloid, triterpenes, polysaccharide, sterol etc.In nutritional labeling, alanine and proline content are high, also contain abundant vitamin C, vitamin B1 and B2.Modern medicine study confirms, Fructus Mume has and protects the liver, antibacterial, antiallergic, the effects such as allaying tiredness.{ Yang Yingfei, Hu Hankun, Liu Ping, Liu Wei, Liu Weizhi; Fructus Mume chemical composition, clinical practice and modern pharmacological research progress; " Chinese pharmacists ", 2012,15 (3): 415-417 }
Pericarpium Citri Reticulatae, claims again Pericarpium Citri tangerinae, is the dry mature skin of rutaceae orange Citrus reticulata Blanco and variety thereof.Medical material is divided into " Pericarpium Citri Reticulatae " and " Pericarpium citri reticuatae chachiensis ".Pluck mature fruit, strip peel, dry or cold drying.Bitter in the mouth, pungent, warm in nature.Return lung, spleen channel.Effect regulating qi-flowing for strengthening spleen, drying dampness to eliminate phlegm.Shennong's Herbal is classified as top grade, records: " tangelo, the contrary gas of the main heat of abdominal mass in the heart, diuretic paddy; Clothes are deodorized for a long time, the therapeutic method to keep the adverse QI flowing downwards, logical god.A Pericarpium Citri tangerinae." Compendium of Material Medica records: " and Fructus Citri tangerinae skin, treats the sound of vomiting regurgitation of vomitting noisy, time vomiting watery fluid, Tan Pi Jie malaria, great Chang Bi plug, married woman's acute mastitis.Enter foodstuff, separate fish raw meat poison.”
The major function composition of Pericarpium Citri Reticulatae is that flavonoid comprises Hesperidin, neohesperidin etc., limonoid comprises limonin, Nomilin etc., volatile oil comprises α-limonene, beta-myrcene etc., also contains alkaloid, phenolic acids, Coumarins, pectin, sterol, inositol etc.In nutritional labeling, contain abundant carotenoid.Modern pharmacological research shows, Pericarpium Citri Reticulatae has gentle stimulation to gastrointestinal smooth muscle, secretion that can facilitating digestion liquid; Meanwhile, also there is anti-inflammation and sterilization, antioxidation, fatigue-resisting function.{ Ou Lijuan, Liu Qide; Pericarpium Citri Reticulatae Advance on Pharmacological Activities; " China Dispensary ", 2006,17 (10): 787-789 }
Fructus Lycii the kidney invigorating and essence nourishing in Chinese medicine composition object space provided by the present invention, nourishing the liver to improve visual acuity, Rhizoma Polygonati nourishing the liver and kidney, spleen invigorating lung moistening; Rhizoma Dioscoreae QI invigorating is reinforced the kidney, strengthening the spleen and nourishing the stomach, and lung benefiting promotes the production of body fluid; Flos Chrysanthemi dispelling wind and heat pathogens, liver heat removing and eyesight improving; Radix Glycyrrhizae heat-clearing and toxic substances removing, invigorating the spleen and replenishing QI, hepatoprotective regulating the spleen and stomach, the mediation property of medicine; Fructus Mume yin fluid astringing tonifying liver, the appetizing of promoting the production of body fluid; Pericarpium Citri Reticulatae regulating qi-flowing for strengthening spleen, soothing the liver circulation of qi promoting.Full side plays enriching yin and nourishing kidney altogether, and the liver benefiting nourishes blood, air making-up and spleen enlivening, and heat clearing away is nourished heart, effect of the lung moistening and production of body fluid promoting.
In order to give Chinese medicine composition various performances, for example improve mouthfeel, aesthetic property or non-oxidizability, except effective ingredient, wherein conventionally can also comprise one or more desirable bromatologies or pharmaceutically acceptable adjuvant, include but not limited to sweeting agent, antioxidant, acidic flavoring agent, excipient and antiseptic, wherein sweeting agent comprises white sugar, glucose, sugar alcohols, sucralose, aspartame, acesulfame potassium, stevioside etc., antioxidant comprises L-AA (vitamin C), L-AA sodium, arabo-ascorbic acid, sodium erythorbate etc., acidic flavoring agent comprises citric acid, malic acid, tartaric acid etc., excipient comprises maltodextrin etc., antiseptic comprises sorbic acid, potassium sorbate etc.
Chinese medicine composition of the present invention can, in being convenient to oral liquid condition or solid state, can be made beverage, oral liquid, granule or tablet.Beverage, oral liquid, granule or tablet can be prepared according to procedures known in the art.
According on the other hand, the invention provides the preparation method of above-mentioned Chinese medicine composition.
The preparation method of Chinese medicine composition of the present invention comprises the steps:
(1) take Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae in prescription ratio, with 5-20, doubly to the water of medical material weight, 80-100 ℃ is extracted 2-3 time, each time 0.5-2 hour;
(2) extracting solution of step (1) is merged, filter, concentrated, obtain concentrated solution 1;
(3) described concentrated solution 1 is filtered, then carry out centrifugalize, discard precipitation, obtain the concentrated liquid of tradition Chinese medicine of clarification.
The preparation method of Chinese medicine composition of the present invention preferably includes following steps:
(1) take Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae in prescription ratio, doubly extract 2-3 time time 0.5-2 hour at every turn with 5-20 to the water of medical material weight at 80-100 ℃;
(2) extracting solution in step (1) is merged, filter, concentrated, obtain concentrated solution 1, by concentrated solution 1 use ethanol precipitation, the supernatant obtaining reclaims ethanol, concentrated, obtains concentrated solution 2;
(3) in described concentrated solution 2, add water, carry out water precipitating processing, then water precipitating liquid is carried out to centrifugalize, discard precipitation, obtain the concentrated liquid of tradition Chinese medicine of clarification.
The preparation method of Chinese medicine composition of the present invention can also preferably include following steps:
(1) take Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae in prescription ratio, doubly extract 2-3 time time 0.5-2 hour at every turn with 5-20 to the water of medical material weight at 80-100 ℃;
(2) extracting solution in step (1) is merged, carry out centrifugal treating, supernatant adopts membrane separation process to filter, and is filtered solution 1;
(3) described filtered solution 1 is adopted reverse osmosis (RO) membrane separation process concentrate, obtain concentrated solution 1, then concentrated solution 1 is carried out to secondary centrifuging processing again, discard precipitation, obtain clarifying the concentrated liquid of tradition Chinese medicine.
According to a preferred embodiment, said method further comprises the steps (4): by the concentrated liquid of tradition Chinese medicine of step (3), spraying is dry, obtains traditional Chinese medicinal extract powder.
According to a preferred embodiment, said method further comprises following step: to the concentrated liquid of tradition Chinese medicine of step (3) or the traditional Chinese medicinal extract powder of step (4), add one or more desirable bromatologies or pharmaceutically acceptable adjuvant, technique is made beverage, oral liquid, granule or tablet routinely.Described adjuvant includes but not limited to sweeting agent, antioxidant, acidic flavoring agent, excipient and antiseptic.
According to another aspect, the invention provides health care and the medical usage of above-mentioned composition.
What the raw material of Chinese medicine composition of the present invention all belonged to the middle regulation of " Ministry of Public Health is about the notice of further standard healthy food material management " (defending method prison sends out [2002] No. 51) is the article of food and medicine.Experiment showed, Chinese medicine composition safety of the present invention through health food Toxicological evaluation again simultaneously, without any side effects.
Chinese medicine composition of the present invention is learned check through function of health food, has alleviating physical fatigue and asthenopic health care.
Chinese medicine composition of the present invention, through pharmacodynamic study, has the memory of improvement and sexual function effect.
Beneficial effect of the present invention:
(1) Chinese medicine composition of the present invention is the raw material of Chinese medicine of integration of edible and medicinal herbs, safe and reliable, without any side effects, and mouthfeel is good, cheap and easy to get.
(2) the present invention combines with Traditional Chinese Medicine health preserving theory and modern medicine study, prescription is reasonable in design, compatibility is rigorous, and action temperature and progressive, has enriching yin and nourishing kidney, the liver benefiting nourishes blood, air making-up and spleen enlivening, heat clearing away is nourished heart, effect of the lung moistening and production of body fluid promoting, the visual fatigue after muscle fatigue, the excess eye-using after lassitude and weak, persistent movement be can effectively eliminate, memory and sexual hypofunction contributed to improve simultaneously.
(3) preparation method of Chinese medicine composition of the present invention combines with traditional extraction process by water and modern advanced isolation technics, has embodied the characteristic of tcm prescription, has greatly retained the various active substances in raw material.
The specific embodiment
Below in conjunction with embodiment, the present invention is elaborated, but do not form any limitation of the invention.
Embodiment 1: the preparation of Chinese medicine composition beverage
1. raw material is prepared: take Fructus Lycii 26kg, Rhizoma Polygonati 18kg, Rhizoma Dioscoreae 18kg, Flos Chrysanthemi 18kg, Radix Glycyrrhizae 8kg, Fructus Mume 8kg and Pericarpium Citri Reticulatae 4kg.
2. purifying: above-mentioned raw materials is joined in conventional extraction element or conventional dynamic extracting device continuously, add 12 times to the water of medical material weight, 90 ℃ are extracted 1.5 hours, obtain water extraction liquid 1, medicinal residues add 10 times to the water of medical material weight, 90 ℃ are extracted 1.0 hours, obtain water extraction liquid 2, medicinal residues add 8 times again to the water of medical material weight, 90 ℃ are extracted 0.5 hour, obtain water extraction liquid 3, merge water extraction liquid 1, 2 and 3, leave standstill, medicinal liquid tubular type continuous high speed centrifugal (15000 revs/min of centrifuge speeds), supernatant tubular membrane is filtered (pump motor frequency 30-50HZ, permeate flow velocity 2-3 ton/hour), filtered solution adopts reverse osmosis (RO) membrance concentration (pump motor frequency 30-50HZ, permeate flow velocity 1-2 ton/hour), obtain concentrated solution, concentrated solution is secondary tubular type continuous high speed centrifugal (15000 revs/min of centrifuge speeds) again, the airtight filter tank filtration sterilization of supernatant, obtain the concentrated liquid of tradition Chinese medicine.
3. allotment and filtration: by above-mentioned the concentrated liquid of tradition Chinese medicine, add 6% glucose, 4% white sugar, 0.03%L-ascorbic acid and 0.02% citric acid, then add gauge water, filter.
4. fill and sterilization: deployed beverage stock solution, fill after instantaneous ultrahigh-temperature sterilization, obtains beverage product.
Embodiment 2: the preparation of Chinese medicine composition oral liquid
1. raw material is prepared: take Fructus Lycii 1kg, Rhizoma Polygonati 1kg, Rhizoma Dioscoreae 1kg, Flos Chrysanthemi 1kg, Radix Glycyrrhizae 15kg, Fructus Mume 15kg and Pericarpium Citri Reticulatae 8kg.
2. purifying: above-mentioned raw materials is joined in conventional extraction element or conventional dynamic extracting device continuously, add 10 times to the water of medical material weight, 90 ℃ are extracted 1.0 hours, obtain water extraction liquid 1, medicinal residues add 8 times to the water of medical material weight, 90 ℃ are extracted 0.5 hour, obtain water extraction liquid 2, medicinal residues add 5 times again to the water of medical material weight, 90 ℃ are extracted 0.5 hour, obtain water extraction liquid 3, merge water extraction liquid 1, 2 and 3, leave standstill, medicinal liquid tubular type continuous high speed centrifugal (15000 revs/min of centrifuge speeds), supernatant tubular membrane is filtered (pump motor frequency 30-50HZ, permeate flow velocity 2-3 ton/hour), filtered solution RO membrance concentration (pump motor frequency 30-50HZ, permeate flow velocity 1-2 ton/hour), obtain concentrated solution, concentrated solution is secondary tubular type continuous high speed centrifugal (15000 revs/min of centrifuge speeds) again, the airtight filter tank filtration sterilization of supernatant, obtain the concentrated liquid of tradition Chinese medicine.
3. allotment and filtration: by above-mentioned the concentrated liquid of tradition Chinese medicine, add 4% white sugar and 0.03% citric acid, then add gauge water, filter.
4. fill and sterilization: after deployed oral liquid stock solution fill, water-bath type high temperature sterilize (105 ℃ sterilizing 30 minutes), obtains oral liquid finished product.
Embodiment 3: the preparation of Chinese medicinal composition granules
1. raw material is prepared: take Fructus Lycii 25kg, Rhizoma Polygonati 15kg, Rhizoma Dioscoreae 15kg, Flos Chrysanthemi 15kg, Radix Glycyrrhizae 0.5kg, Fructus Mume 0.5kg and Pericarpium Citri Reticulatae 0.5kg.
2. purifying: above-mentioned raw materials is joined in conventional extraction element or conventional dynamic extracting device continuously, add 15 times to the water of medical material weight, 95 ℃ are extracted 2.0 hours, obtain water extraction liquid 1, medicinal residues add 12 times to the water of medical material weight, 95 ℃ are extracted 1.0 hours, obtain water extraction liquid 2, medicinal residues add 10 times again to the water of medical material weight, 95 ℃ are extracted 1.0 hours, obtain water extraction liquid 3, merge water extraction liquid 1, 2 and 3, leave standstill, be evaporated to medicinal liquid proportion 1.09-1.13 (70-80 ℃ of survey), after plate-and-frame filtration, medicinal liquid tubular type continuous high speed centrifugal (15000 revs/min of centrifuge speeds), discard precipitation, obtain supernatant, supernatant adds 20% maltodextrin, spraying is dry, collect traditional Chinese medicinal extract powder.
3. granulating and forming and packing: above-mentioned traditional Chinese medicinal extract powder, add 40% Icing Sugar, boiling granulating, sieves, and subpackage obtains granule finished product.
Embodiment 4: the preparation of Chinese medicine composition composition chewable tablet
1. raw material is prepared: take Fructus Lycii 2kg, Rhizoma Polygonati 15kg, Rhizoma Dioscoreae 15kg, Flos Chrysanthemi 15kg, Radix Glycyrrhizae 2kg, Fructus Mume 2kg and Pericarpium Citri Reticulatae 1kg.
2. purifying: above-mentioned raw materials is joined in conventional extraction element or conventional dynamic extracting device continuously, add 10 times to the water of medical material weight, 95 ℃ are extracted 1.5 hours, obtain water extraction liquid 1, medicinal residues add 8 times to the water of medical material weight, 95 ℃ are extracted 1.0 hours, obtain water extraction liquid 2, merge water extraction liquid 1 and 2, leave standstill, be evaporated to medicinal liquid proportion 1.10-1.15 (70-80 ℃ of survey), plate-and-frame filtration, concentrated solution adds 95% ethanol to carry out precipitate with ethanol (60%) processing, the alcohol supernatant that obtains overnight; Above-mentioned alcohol liquid is reclaimed to ethanol, be evaporated to medicinal liquid proportion 1.20-1.25 (70-80 ℃ of survey), add 0.5 times of amount pure water, cool to room temperature, cold preservation (4-10 ℃) the water precipitating liquid that obtains overnight; Above-mentioned water precipitating liquid tubular type continuous high speed centrifugal (15000 revs/min of centrifuge speeds), discards precipitation, obtains supernatant, and supernatant adds 30% maltodextrin, and spraying is dry, collects traditional Chinese medicinal extract powder.
3. granulate: above-mentioned traditional Chinese medicinal extract powder, add 30% Icing Sugar, 10% pregelatinized Starch and 0.1% aspartame, boiling granulating, sieves, and obtains granule.
4. tabletting and subpackage: above-mentioned granule, add 1.5% micropowder silica gel, tabletting, subpackage, obtains tablet finished product.
Embodiment 5: Chinese medicine composition chmice acute Oral toxicity experiment
One, detect foundation: Ministry of Public Health " health food check and assessment technique standard " (version in 2003)
Two, materials and methods
1. in given the test agent processing: embodiment 1 in the concentrated liquid of tradition Chinese medicine of step 2 (hereinafter to be referred as concentrated solution 1.) and embodiment 3 traditional Chinese medicinal extract powder (hereinafter to be referred as extract powder 3.) of step 2 respectively adding distil water be diluted to 1ml sample and be equivalent to 1g raw material of Chinese medicine.
2. experimental animal: Kunming mouse, male and female half and half, body weight (20 ± 2) g, is provided production licence number by Shanghai Slac Experimental Animal Co., Ltd.: SCXK (Shanghai) 2012-0002.20 ℃-25 ℃ of receptacle temperature, relative humidity 40%-70%.Mouse feed is provided by Suzhou two lion laboratory animal feed technology Services Co., Ltd, registration card number: Soviet Union raises careful (2009) 05032.
3. experimental technique:
(1) animal fasting (can't help water), after 16 hours, is selected each 10 of female, male mice by body weight requirement, divides and is put in two mouse cages, is no more than 3g with the difference of body weight between sex mice.
(2) given the test agent is adopted maximum tolerated dose method to contaminate to laboratory animal, mice is by only weighing, and gavage capacity is by each 0.4ml/20g weighing machine, in 24 hours 2 times to mouse stomach, be 6 hours gavage interval time of 2 times.
(3) after contamination, observe general state, body weight change, poisoning symptom and the death condition etc. of animal.Observation period is two weeks.
(4) testing end weighs to animal again.Dead animal and the animal of putting to death that expires are carried out to obduction, and perusal general pathology changes situation.
(5) test overall process and observed content and all do itemized record, try to achieve chmice acute per os MTD by maximum tolerated dose method result of the test.
4. experimental result:
(1) main physical signs performance: the each treated animal of duration of test is movable normal, and hair color glossiness is good, has no any poisoning sign and death; Expire and put to death animal, the each internal organs situation of gross anatomy perusal, no abnormality seen.
(2) female mice: MTD>40g raw material of Chinese medicine/kg; Male mice: MTD>40g Chinese medicine composition/kg.
5. experiment conclusion:
3. 1. concentrated solution be all greater than 40g raw material of Chinese medicine/kg to the maximum tolerated dose MTD of female, male chmice acute Oral toxicity test with extract powder, belongs to nontoxic level.
Embodiment 6: Chinese medicine composition alleviating physical fatigue functional experiment
One, detect foundation: Ministry of Public Health " health food check and assessment technique standard " (version in 2003)
Two, materials and methods
1. experimental animal: Kunming mouse, male, body weight (20 ± 2) g, is provided production licence number by Shanghai Slac Experimental Animal Co., Ltd.: SCXK (Shanghai) 2012-0002.20 ℃-25 ℃ of receptacle temperature, relative humidity 40%-70%.Mouse feed is provided by Suzhou two lion laboratory animal feed technology Services Co., Ltd, registration card number: Soviet Union raises careful (2009) 05032.
2. dosage design: the traditional Chinese medicinal extract powder (hereinafter to be referred as extract powder 3.) of step 2 in the concentrated liquid of tradition Chinese medicine of step 2 (hereinafter to be referred as concentrated solution 1.) and embodiment 3 in embodiment 1, human body recommended dose is 24g raw material of Chinese medicine/60kg every day, middle and high two dosage groups are established in this experiment, be respectively 4g raw material of Chinese medicine/kg, 8g raw material of Chinese medicine/kg, be equivalent to 10,20 times of human body recommended dose, separately establish distilled water blank group.
3. given the test agent processing: concentrated solution 1. with extract powder 3. respectively adding distil water be diluted to 1ml sample and be equivalent to 0.2g raw material of Chinese medicine, 1ml sample is equivalent to 0.4g raw material of Chinese medicine, is the test liquid of middle and high two dosage groups.
4. route of administration: gavage, gavage capacity 0.4ml/20g body weight.
5. statistical method: experimental result is all carried out variance analysis with SPSS13.0 version software.
6. experimental technique and result
Get 150 of mices, by only weighing, be divided into 15 groups by body weight stratified random, 10 every group.Take large group of every 5 groups as one experiment, conduct an experiment.All animals are according to dosage designed continuous nursing 30 days, weigh weekly, adjust to sample amount according to body weight.
6.1 Loaned swimming tests:
After last gavage 30 minutes, mice is put into water went swimming, depth of water 30cm, 25 ℃ ± 1 ℃ of water temperature, the mouse tail root 5% body weight sheet lead of loading, records mice and starts to the dead time from swimming.The results are shown in Table 1.
The impact of table 1 sample on the mice burden swimming time
Note: * P<0.05, with matched group comparison
As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference; 1. 3. high dose group is compared with matched group with extract powder for concentrated solution, and the swimming with a load attached to the body time obviously extends, and has significant difference.
6.2 serum ureas are measured:
After last gavage 30 minutes, mice is put into 30 ℃ of not swimmings with a load attached to the body of water 90 minutes, taking-up is dried, and the blood sampling after 60 minutes of having a rest is centrifugal, gets serum and surveys carbamide.The results are shown in Table 2.
Table 2 sample on mouse movement after the impact of serum urea
Note: * P<0.05, with matched group comparison
As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference; 1. 3. high dose group is compared with matched group with extract powder for concentrated solution, and serum urea level obviously reduces, and has significant difference.
6.3 hepatic glycogen are measured
Within 30 minutes after last gavage, put to death mice, get at once liver, wipe dry after rinsing in normal saline, detects hepatic glycogen.The results are shown in Table 3.
The impact of table 3 sample on Mouse Liver glycogen
Note: * P<0.05, with matched group comparison
As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference; 1. 3. high dose group is compared with matched group with extract powder for concentrated solution, and hepatic glycogen level obviously increases, and has significant difference.
7. conclusion: under this experimental condition, the Loaned swimming test result positive, and serum urea, the hepatic glycogen index positive, therefore think that 3. 1. concentrated solution have alleviating physical fatigue function to mice with extract powder.
Embodiment 7: Chinese medicine composition asthenopia releasing function experiment
One, detect foundation: " about the notice of printing and distributing 9 health care evaluation methodologys such as anti-oxidation function evaluation methodology " (No. [2012] 107, state's food medicine prison guarantorization) adnexa 4 asthenopia releasing function evaluation methodologys.
Two, experimenter's inclusive criteria: selecting the age by the principle of voluntariness is 18-65 year, long-term with eye, 120 of the fatigable experimenters of vision.
Three, experimenter's exclusion standard: suffer from infectivity, traumatic ocular disease person; Carry out 3 months persons of operated eye less than; Suffers from the inside and outside eye disease patient such as cornea, crystal, vitreous body, retinopathy; Suffer from the disease patients such as cardiovascular, cerebrovascular, liver, kidney, hemopoietic system; Gestation or women breast-feeding their children and allergic constitution patient; Take in a short time the article relevant with tested function, the judgement person of impact to result; Long-term taking is about the medicine, health food for the treatment of vision or use other treatment method to fail terminator; Do not meet inclusive criteria, edible tested material person or data are not congruent in accordance with regulations affects effect or safety judgement person.
Four, test method
1. test grouping: 120 routine experimenters adopt random double-blind method to be divided into test-meal group and placebo group, every group of each 60 examples, the factors such as age, sex, vision have comparability, between test employing self cross-reference and group, contrast.
2. test-meal method: test-meal group is taken beverage in embodiment 1 (hereinafter to be referred as beverage 1.), and matched group is taken placebo, is every day 2 times, and each 1 bottle, Time of Administration is 60 days.
3. observation index: indices is each mensuration 1 time in the time of test-meal on-test and end.
3.1 safety indexes: general physical examination blood, urine, feces routine, the biochemical indicator of having a blood test, Chest X-rays, electrocardiogram, ultrasound diagnosis, untoward reaction is observed.
3.2 efficiency indexes: check in the time that test-meal starts and finish.
3.2.1 questionnaire survey: an eye situation is inquired, used to symptom.
3.2.2 ophthalmologic examination: comprise examination of ocular fundus, examination of visual acuity (myopia, hypermetropia, astigmatism etc.).
3.2.3 duration of photopic vision: the photopic vision time is called duration of photopic vision to the percentage ratio of fixation time.
3.3 effect criterion
3.3.1 duration of photopic vision: test-meal group self relatively or between test-meal group and matched group group compares, and duration of photopic vision difference has significance (p<0.05), and average duration of photopic vision raising is more than or equal to 10% for effective.
3.3.2 symptom average integral: calculate the symptom integral of every examination trencherman test-meal front and back, calculate respectively the average product score value of two groups, and carry out statistical test.
Table 4 asthenopia symptom decision method (sxemiquantitative integration method)
Note: " random thoughts " refer to 1-2 time/2 days; " time have " refers to 1-3 times/day; " often " refers to >3 times/day
4. statistical method: SPSS13.0 version software kit is all used in data statistics, enumeration data adopts chi-square criterion, and measurement data adopts t check.
Five, result of the test
1. the physical data before experimenter's experiment: observe altogether 120 routine asthenopes, be divided at random test-meal group, each 60 examples of matched group.When test-meal finishes, test-meal group 7 examples are check on time not, has 3 examples to drop by the wayside; Matched group has the not check on time of 8 examples, and 2 examples are dropped by the wayside.Test-meal group and matched group respectively have 10 examples to meet experimenter's exclusion standard, and the de-mistake rate of case is 16.67%.Meet test-meal group 50 examples of inclusive criteria, wherein male's 12 examples, women's 38 examples.Matched group 50 examples, wherein male's 11 examples, women's 39 examples.All experimenter is through interior eye, examination of external eye normal.The front two groups of indices no significant differences of test-meal (P>0.05).Specifically in table 5.
Physical data comparison before table 5 test-meal
| Group | Effectively number of cases | Age (year) | Duration of photopic vision (%) | Symptom integral |
| Test-meal group | 50 | 46.50±6.79 | 56.59±4.59 | 10.22±2.33 |
| Matched group | 50 | 48.32±7.22 | 57.32±4.48 | 10.24±2.35 |
2. duration of photopic vision changes: specifically in table 6
Before and after table 6 test-meal, duration of photopic vision changes statistics
| Group | Effectively number of cases | Before test-meal (%) | After test-meal (%) | Improve (%) |
| Test-meal group | 50 | 56.59±4.59 | 66.96±4.91##** | 10.37±3.54** |
| Matched group | 50 | 57.32±4.48 | 58.84±4.81 | 1.52±1.98 |
Before note: ## and test-meal, self compares P<0.01, and * * and matched group be P<0.01 relatively.
3. symptom integral changes: specifically in table 7.
Symptom integral statistics (± SD) before and after table 7 test-meal
| Group | Effectively number of cases | Before test-meal | After test-meal | Difference |
| Test-meal group | 50 | 10.46±2.42 | 7.20±2.11##** | 3.26±1.63** |
| Matched group | 50 | 10.28±2.27 | 9.56±1.80 | 0.72±1.11 |
Before note: ## and test-meal, self compares P<0.01, and * * and matched group be P<0.01 relatively.
Six. conclusion:
Under this research, test-meal group duration of photopic vision, test-meal front and back self comparing difference has significance (P<0.01), after test-meal, duration of photopic vision improves 10.37%, be greater than 10%, and with matched group test-meal after duration of photopic vision comparing difference have significance (P<0.01); Test-meal group symptom integral, self comparing difference has significance (P<0.01) before and after test-meal, and after test-meal, symptom integral subtracts score value, subtracts score value comparing difference have significance (P<0.01) with matched group symptom integral.Therefore think that 1. beverage has function of relieving visual fatigue to human body.
Embodiment 8: Chinese medicine composition auxiliary improving memory function experiment
One, detect foundation: Ministry of Public Health " health food check and assessment technique standard " (version in 2003)
Two, materials and methods
1. experimental animal: Kunming mouse, male, body weight (20 ± 2) g, is provided production licence number by Shanghai Slac Experimental Animal Co., Ltd.: SCXK (Shanghai) 2012-0002.20 ℃-25 ℃ of receptacle temperature, relative humidity 40%-70%.Mouse feed is provided by Suzhou two lion laboratory animal feed technology Services Co., Ltd, registration card number: Soviet Union raises careful (2009) 05032.
2. dosage design: the traditional Chinese medicinal extract powder (hereinafter to be referred as extract powder 3.) of step 2 in the concentrated liquid of tradition Chinese medicine of step 2 (hereinafter to be referred as concentrated solution 1.) and embodiment 3 in embodiment 1, human body recommended dose is 24g raw material of Chinese medicine/60kg every day, middle and high two dosage groups are established in this experiment, be respectively 4g raw material of Chinese medicine/kg, 12g raw material of Chinese medicine/kg, be equivalent to 10,30 times of human body recommended dose, separately establish distilled water blank group.
3. given the test agent processing: concentrated solution 1. with extract powder 3. respectively adding distil water be diluted to 1ml sample and be equivalent to 0.2g raw material of Chinese medicine, 1ml sample is equivalent to 0.6g raw material of Chinese medicine, is the test liquid of middle and high two dosage groups.
4. route of administration: gavage, gavage capacity 0.4ml/20g body weight.
5. statistical method: experimental result is all carried out variance analysis with SPSS13.0 version software.
6. experimental technique and result
Get 100 of mices, by only weighing, be divided into 10 groups by body weight stratified random, 10 every group.Take large group of every 5 groups as one experiment, conduct an experiment.All animals are according to dosage designed continuous nursing 45 days, weigh weekly, adjust to sample amount according to body weight.
6.1 diving tower experiments:
After last gavage, start to train next day.Mice is put into reaction chamber endoadaptation 3 minutes, then, by the copper grid in mice placing response case, pass to immediately 36 volts of alternating currents, the normal reaction of mice is to jump back on insulated platform.Most animals may skip on copper grid again or repeatedly, is subject to the platform that snaps back again that shocks by electricity.After training once, animal is placed on the platform in reaction chamber, records the incubation period that in 5 minutes, each Mus jumps off the errors number of platform and jumps off for the first time platform, using this as school grade.The same time after 24h, carry out cyclical test, mice is placed on platform, record the number of animals sum that each Mus jumps off for the first time number of shocks in the incubation period of platform, each Mus 3 minutes and shocked by electricity, calculate the percentage rate (number of animals being shocked by electricity accounts for the percentage rate of this treated animal sum) of the animal that occurs wrong reaction simultaneously.The results are shown in Table 8.
The impact of table 8 sample on the experiment of mice diving tower
Note: * P<0.05, with matched group comparison
As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference; 1. 3. high dose group is compared with matched group with extract powder for concentrated solution, and obviously extend the incubation period of jumping off platform, has significant difference.
6.2 keep away dark experiment:
After last gavage, start to train next day.When experiment, mice face is put into bright chamber in hole dorsad, start timer simultaneously, mice enters darkroom through hole and is shocked by electricity, timer stops, take out mice, record every Mus and suffer required time of electric shock, i.e. incubation period from putting into bright chamber to entering darkroom, train 5 minutes, and record number of shocks in 5 minutes.The same time after 24h, carry out cyclical test, record every Mus and enter the number of shocks in incubation period and 5 minutes in darkroom, and calculate the animal percentage rate that enters darkroom (wrong reaction) in 5 minutes.The results are shown in Table 9.
Table 9 sample is kept away the impact of dark experiment on mice
Note: * P<0.05, with matched group comparison
As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference; 1. 3. high dose group is compared with matched group with extract powder for concentrated solution, and obviously extend the incubation period that enters darkroom, has significant difference.
7. conclusion: under this experimental condition, diving tower is tested and kept away the dark experimental result positive, therefore think that 3. 1. concentrated solution have auxiliary improving memory function to mice with extract powder.
Embodiment 9: the impact of Chinese medicine composition on castrated rats penile erectile function and reproductive development
One, experimental basis: Qi Chen edits " herbal pharmacology research methodology "
Two, materials and methods
1. experimental animal: Wistar rat, male, body weight 100g, is provided by Shanghai Slac Experimental Animal Co., Ltd., production licence number: SCXK(Shanghai) 2012-0002.20 ℃-25 ℃ of receptacle temperature, relative humidity 40%-70%.Rat feed is provided by Suzhou two lion laboratory animal feed technology Services Co., Ltd, registration card number: Soviet Union raises careful (2009) 05032.
2. the foundation of model: 50 male rats, use ether light anaesthesia, routine disinfection, excision both sides testis.In second day after operation random packet.
3. dosage design: the traditional Chinese medicinal extract powder (hereinafter to be referred as extract powder 3.) of step 2 in the concentrated liquid of tradition Chinese medicine of step 2 (hereinafter to be referred as concentrated solution 1.) and embodiment 3 in embodiment 1, human body recommended dose is 24g raw material of Chinese medicine/60kg every day, middle and high two dosage groups are established in this experiment, be respectively 2g raw material of Chinese medicine/kg, 6g raw material of Chinese medicine/kg, be equivalent to 5,15 times of human body recommended dose, separately establish distilled water blank group.
4. given the test agent processing: concentrated solution 1. with extract powder 3. respectively adding distil water be diluted to 1ml sample and be equivalent to 0.4g raw material of Chinese medicine, 1ml sample is equivalent to 1.2g raw material of Chinese medicine, is the test liquid of middle and high two dosage groups.
5. route of administration: gavage, gavage capacity 0.5mL/100g body weight.
6. statistical method: experimental result is all carried out variance analysis with SPSS13.0 version software.
7. experimental technique and result
Get 50 of rats, by only weighing, be divided into 5 groups by body weight stratified random, 10 every group.All animals are according to dosage designed continuous nursing 45 days, weigh weekly, adjust to sample amount according to body weight.1h after last gavage, is positioned over rat penis position by the stimulating electrode of electrostimulator, gives surface element electricity irritation, and record starts to erection time (erecing incubation period) from stimulating; Again rat is put to death and dissected each group of rat, take immediately preputial glands, seminal vesicle+and prostatic weight, calculate organ index.The results are shown in Table 10.
The impact of table 10 sample on castrated rats penile erectile function and reproductive development
Note: * P<0.05, with matched group comparison
As seen from the above table, between each group, the weight of animals is in test just and there are no significant at test end difference; Concentrated solution 1. with extract powder 3. high dose group compared with matched group, can obviously shorten castrated rats eclipse period of penile erection, can obviously promote the growth promoter of castrated rats preputial glands, prostate and seminal fluid capsule simultaneously, have significant difference.
8. conclusion: under this experimental condition, the result positive that castrated rats penile erectile function experiment and reproductive development are tested, therefore think that 3. 1. concentrated solution can assist and improve castrated rats sexual function with extract powder.
Finally it should be noted that: above-described embodiment is only for example of the present invention is clearly described, and the not restriction to embodiment.For those of ordinary skill in the field, can also make on the basis of the above description other multi-form variation or variations.Here without also giving exhaustive to all embodiments.And the apparent variation of being amplified out thus or variation are still among protection scope of the present invention.
Claims (10)
1. the Chinese medicine composition of a fatigue alleviating, its active component is made up of Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae, wherein, the weight proportion of Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae is: Fructus Lycii 1-50 part, Rhizoma Polygonati 1-30 part, Rhizoma Dioscoreae 1-30 part, Flos Chrysanthemi 1-30 part, Radix Glycyrrhizae 1-15 part, Fructus Mume 1-15 part and Pericarpium Citri Reticulatae 1-8 part.
2. Chinese medicine composition as claimed in claim 1, is characterized in that, described weight proportion is: Fructus Lycii 2-25 part, Rhizoma Polygonati 2-15 part, Rhizoma Dioscoreae 2-15 part, Flos Chrysanthemi 2-15 part, Radix Glycyrrhizae 2-9 part, Fructus Mume 2-9 part and Pericarpium Citri Reticulatae 1-5 part.
3. Chinese medicine composition as claimed in claim 2, is characterized in that, described weight proportion is: 2 parts of 13 parts of Fructus Lycii, 9 parts of Rhizoma Polygonatis, 9 parts of Rhizoma Dioscoreaes, 9 parts of Flos Chrysanthemis, 4 parts, Radix Glycyrrhizae, 4 parts of Fructus Mumes and Pericarpium Citri Reticulataes.
4. the Chinese medicine composition as described in any one in claim 1-3, is characterized in that, described Chinese medicine composition also comprises one or more bromatologies or pharmaceutically acceptable adjuvant.
5. a method of preparing the Chinese medicine composition as described in any one in claim 1-3, comprises step:
(1) take Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae in prescription ratio, with 5-20, doubly to the water of medical material weight, 80-100 ℃ is extracted 2-3 time, each time 0.5-2 hour;
(2) extracting solution of step (1) is merged, filter, concentrated, obtain concentrated solution 1;
(3) described concentrated solution 1 is filtered, then carry out centrifugalize, discard precipitation, obtain the concentrated liquid of tradition Chinese medicine of clarification.
6. a method of preparing the Chinese medicine composition as described in any one in claim 1-3, comprises step:
(1) take Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae in prescription ratio, doubly extract 2-3 time time 0.5-2 hour at every turn with 5-20 to the water of medical material weight at 80-100 ℃;
(2) extracting solution in step (1) is merged, filter, concentrated, obtain concentrated solution 1, by concentrated solution 1 use ethanol precipitation, the supernatant obtaining reclaims ethanol, concentrated, obtains concentrated solution 2;
(3) in described concentrated solution 2, add water, carry out water precipitating processing, then water precipitating liquid is carried out to centrifugalize, discard precipitation, obtain the concentrated liquid of tradition Chinese medicine of clarification.
7. a method of preparing the Chinese medicine composition as described in any one in claim 1-3, comprises step:
(1) take Fructus Lycii, Rhizoma Polygonati, Rhizoma Dioscoreae, Flos Chrysanthemi, Radix Glycyrrhizae, Fructus Mume and Pericarpium Citri Reticulatae in prescription ratio, doubly extract 2-3 time time 0.5-2 hour at every turn with 5-20 to the water of medical material weight at 80-100 ℃;
(2) extracting solution in step (1) is merged, carry out centrifugal treating, supernatant adopts membrane separation process to filter, and is filtered solution 1;
(3) described filtered solution 1 is adopted reverse osmosis (RO) membrane separation process concentrate, obtain concentrated solution 1, then concentrated solution 1 is carried out to secondary centrifuging processing again, discard precipitation, obtain clarifying the concentrated liquid of tradition Chinese medicine.
8. the method as described in any one in claim 5-7, further comprises the steps (4): by the concentrated liquid of tradition Chinese medicine of step (3), spraying is dry, obtains traditional Chinese medicinal extract powder.
9. the method as described in any one in claim 5-7 or claim 8, further comprise the steps: to the concentrated liquid of tradition Chinese medicine of step (3) or the traditional Chinese medicinal extract powder of step (4), add one or more bromatologies or pharmaceutically acceptable adjuvant, technique is made beverage, oral liquid, granule or tablet routinely.
10. the purposes of the Chinese medicine composition described in any one in health product or medicine for the preparation of alleviating physical fatigue and asthenopia and improvement memory and sexual function in claim 1-3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410160138.8A CN103893549B (en) | 2014-04-21 | 2014-04-21 | A kind of Chinese medicine composition of fatigue alleviating and its production and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410160138.8A CN103893549B (en) | 2014-04-21 | 2014-04-21 | A kind of Chinese medicine composition of fatigue alleviating and its production and use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103893549A true CN103893549A (en) | 2014-07-02 |
| CN103893549B CN103893549B (en) | 2017-01-04 |
Family
ID=50985333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410160138.8A Active CN103893549B (en) | 2014-04-21 | 2014-04-21 | A kind of Chinese medicine composition of fatigue alleviating and its production and use |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103893549B (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104721475A (en) * | 2015-04-16 | 2015-06-24 | 朱国强 | Healthcare product for relieving physical fatigue and preparation method of healthcare product for relieving physical fatigue |
| CN105311383A (en) * | 2015-07-06 | 2016-02-10 | 厦门医学高等专科学校 | Healthcare traditional Chinese medicine composition with functions of asthenopia prevention and improvement and preparation method of healthcare traditional Chinese medicine composition with functions of asthenopia prevention and improvement |
| CN105410515A (en) * | 2015-10-23 | 2016-03-23 | 王海平 | Beverage formula rich in nutrition |
| CN105456822A (en) * | 2016-01-13 | 2016-04-06 | 裴昌松 | Health-care millet wine for replenishing yin and strengthening yang |
| CN105796749A (en) * | 2016-03-03 | 2016-07-27 | 中国人民解放军总医院第附属医院 | Acupoint massage and traditional Chinese medicine composition for relieving asthenopia |
| CN106177061A (en) * | 2016-08-29 | 2016-12-07 | 黑龙江德能理疗技术开发有限责任公司 | A kind of fatigue alleviating oral liquid and preparation method thereof |
| CN106343544A (en) * | 2016-06-08 | 2017-01-25 | 安徽华瑞医药技术开发有限公司 | Functional food capable of relieving chronic fatigue syndrome |
| CN107535769A (en) * | 2016-06-23 | 2018-01-05 | 黄然 | A kind of energy drink for contributing to body-building and effect of weight reducing |
| CN108926671A (en) * | 2018-08-28 | 2018-12-04 | 岚皋县硒安生物科技有限公司 | Integration of drinking and medicinal herbs Chinese medicine composition and its application, integration of drinking and medicinal herbs health care product |
| CN109430871A (en) * | 2018-11-29 | 2019-03-08 | 高锦龙 | A kind of beverage and preparation method thereof |
| CN110179966A (en) * | 2019-05-17 | 2019-08-30 | 严启新 | The preparation method and applications of traditional Chinese medicine composition for relieving asthenopia |
| CN110226721A (en) * | 2019-04-29 | 2019-09-13 | 潘元风 | A kind of composition with auxiliary hyperglycemic function |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283979A (en) * | 2011-09-08 | 2011-12-21 | 惠州缔康生物科技有限公司 | Oral liquid containing cuora trifasciata and cordyceps |
| CN102406020A (en) * | 2011-11-15 | 2012-04-11 | 李永胜 | Chinese wolfberry and chrysanthemum anti-fatigue fire toxin-releasing king tea oral liquid |
| CN102824548A (en) * | 2012-09-19 | 2012-12-19 | 北京亚东生物制药有限公司 | Traditional Chinese medicine composition for treating asthenopia and preparation method thereof |
-
2014
- 2014-04-21 CN CN201410160138.8A patent/CN103893549B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283979A (en) * | 2011-09-08 | 2011-12-21 | 惠州缔康生物科技有限公司 | Oral liquid containing cuora trifasciata and cordyceps |
| CN102406020A (en) * | 2011-11-15 | 2012-04-11 | 李永胜 | Chinese wolfberry and chrysanthemum anti-fatigue fire toxin-releasing king tea oral liquid |
| CN102824548A (en) * | 2012-09-19 | 2012-12-19 | 北京亚东生物制药有限公司 | Traditional Chinese medicine composition for treating asthenopia and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 杜朝辉等: "抗运动性疲劳中药研究现状", 《中国运动医学杂志》 * |
| 沈红梅等: "乌梅的化学、药理及临床研究进展", 《中成药》 * |
| 赵小艳等: "陈皮的研究概况", 《中国药业》 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104721475A (en) * | 2015-04-16 | 2015-06-24 | 朱国强 | Healthcare product for relieving physical fatigue and preparation method of healthcare product for relieving physical fatigue |
| CN104721475B (en) * | 2015-04-16 | 2018-09-25 | 朱国强 | A kind of health care product used for relieving physical fatigue and preparation method thereof |
| CN105311383A (en) * | 2015-07-06 | 2016-02-10 | 厦门医学高等专科学校 | Healthcare traditional Chinese medicine composition with functions of asthenopia prevention and improvement and preparation method of healthcare traditional Chinese medicine composition with functions of asthenopia prevention and improvement |
| CN105311383B (en) * | 2015-07-06 | 2019-11-08 | 厦门医学院 | A kind of health-care Chinese medicinal composition and preparation method thereof of prevention and improvement visual fatigue |
| CN105410515A (en) * | 2015-10-23 | 2016-03-23 | 王海平 | Beverage formula rich in nutrition |
| CN105456822A (en) * | 2016-01-13 | 2016-04-06 | 裴昌松 | Health-care millet wine for replenishing yin and strengthening yang |
| CN105456822B (en) * | 2016-01-13 | 2019-02-22 | 裴昌松 | One kind educating yin and helps positive Health care yellow wine |
| CN105796749B (en) * | 2016-03-03 | 2018-05-22 | 中国人民解放军总医院第一附属医院 | A kind of traditional Chinese medicine composition for relieving asthenopia |
| CN105796749A (en) * | 2016-03-03 | 2016-07-27 | 中国人民解放军总医院第附属医院 | Acupoint massage and traditional Chinese medicine composition for relieving asthenopia |
| CN106343544A (en) * | 2016-06-08 | 2017-01-25 | 安徽华瑞医药技术开发有限公司 | Functional food capable of relieving chronic fatigue syndrome |
| CN107535769A (en) * | 2016-06-23 | 2018-01-05 | 黄然 | A kind of energy drink for contributing to body-building and effect of weight reducing |
| CN106177061A (en) * | 2016-08-29 | 2016-12-07 | 黑龙江德能理疗技术开发有限责任公司 | A kind of fatigue alleviating oral liquid and preparation method thereof |
| CN108926671A (en) * | 2018-08-28 | 2018-12-04 | 岚皋县硒安生物科技有限公司 | Integration of drinking and medicinal herbs Chinese medicine composition and its application, integration of drinking and medicinal herbs health care product |
| CN109430871A (en) * | 2018-11-29 | 2019-03-08 | 高锦龙 | A kind of beverage and preparation method thereof |
| CN110226721A (en) * | 2019-04-29 | 2019-09-13 | 潘元风 | A kind of composition with auxiliary hyperglycemic function |
| CN110179966A (en) * | 2019-05-17 | 2019-08-30 | 严启新 | The preparation method and applications of traditional Chinese medicine composition for relieving asthenopia |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103893549B (en) | 2017-01-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103893549B (en) | A kind of Chinese medicine composition of fatigue alleviating and its production and use | |
| US10576120B2 (en) | Chinese herbal oral paste for conditioning qi deficiency constitution and processing method therefor | |
| CN102172269B (en) | Hidegelatin fabricated food with beautification function | |
| US10485832B2 (en) | Chinese herbal oral paste for conditioning qi depression constitution and processing method therefor | |
| CN108926702A (en) | A kind of Chinese herbal medicine formula and preparation method thereof for conditioning subhealth state | |
| CN107853517B (en) | Traditional Chinese medicine beverage capable of refreshing and resisting fatigue and preparation method thereof | |
| CN103041209A (en) | Chinese herbal preparation for curing deficiency of kidney-yin and preparation method thereof | |
| CN108813501A (en) | With the relieving cough and reducing sputum health honey paste relievingd asthma and adjust function of human body of clearing heat and moistening lung | |
| KR100688252B1 (en) | Composition for improving male sexual function | |
| CN103028047B (en) | Chinese patent medicine for treating chronic pharyngolaryngitis | |
| CN105148220A (en) | Traditional Chinese medicine for treating spleen-kidney yang deficiency type dizziness and preparation method of traditional Chinese medicine | |
| CN104397282A (en) | Tranquilization and brain tonifying eleutherococcus senticosus healthare tea and preparation method thereof | |
| CN104888158A (en) | Folium apocyni veneti oral liquid for enhancing immunity and preparation method thereof | |
| CN104324329A (en) | Mulberry oral liquid with effects of soothing nerves and nourishing brain and preparation method of mulberry oral liquid | |
| CN114712219A (en) | Acupoint pressure stimulation patch with effect of conditioning infantile pneumonia and cough | |
| CN103520302A (en) | Sea-buckthorn leaf anti-fatigue capsule and preparation method thereof | |
| CN107183426B (en) | Liver-protecting and alcohol-dispelling plant beverage, and preparation method and application thereof | |
| CN103720808A (en) | Composition with constipation relieving function and application and preparation method thereof | |
| CN111670977B (en) | Qi-regulating spleen-strengthening health-care tea | |
| KR102632013B1 (en) | A composition containing herbs extract, and manufacturing method of the same | |
| CN109106842B (en) | A medicine for stopping smoking and its preparation method | |
| CN106729584A (en) | It is a kind of to treat Chinese medicine composition of baby anorexia and preparation method thereof | |
| KR20100035961A (en) | Composition of gongjindan comprising nono powder of jinseung | |
| CN104491349A (en) | Medicine for treating convulsions caused by fear and preparation method of medicine | |
| CN105031286A (en) | Chinese herb preparation for treating iron-deficiency anemia and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |