CN103875923B - The biology enzyme of fat coating or probiotics - Google Patents
The biology enzyme of fat coating or probiotics Download PDFInfo
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- CN103875923B CN103875923B CN201410134978.7A CN201410134978A CN103875923B CN 103875923 B CN103875923 B CN 103875923B CN 201410134978 A CN201410134978 A CN 201410134978A CN 103875923 B CN103875923 B CN 103875923B
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Abstract
The invention discloses a kind of biology enzyme or probiotics of fatty coating, will be first that the boil down to fluid storage such as solvent liquefaction oil gas of gaseous state is in storage tank under normal temperature and pressure; Coating material is placed in dissolution kettle, vacuumizes, enter in dissolution kettle by the liquid flux hydrocarbon infusion in storage tank, liquid flux is heated up becomes subcritical fluids, stirs and makes coating material dissolves; Enzyme preparation or probiotics is added in encapsulating still, vacuumize after sealing, drive hydrocarbon pump and the solution containing coating material in dissolution kettle is injected encapsulating still, decompression release solvent, the material temperature controlled in encapsulating still is no more than 35 DEG C, when in encapsulating still, pressure is to normal pressure, vacuumize, then emptying makes in encapsulating still that pressure is to normal pressure, and discharging, namely obtains coating enzyme preparation or probiotics finished product.The invention has the advantages that whole process can complete under the condition close to room temperature, anaerobic, greatly reduce enzyme preparation and probiotics inactivation in process, improve the activity of product.
Description
Technical field
The present invention relates to feed addictive---biology enzyme and probiotics, especially relate to a kind of biology enzyme or probiotics of fatty coating.
Background technology
Enzyme is the protein with catalytic activity, has high efficiency, selectivity, is extensively present in animals and plants and microbial body, is that organism maintains the requisite composition of normal physiological biochemical function.Poultry, the utilization of domestic animal to feed Middle nutrition material is absorbed after various macromolecular substances being degraded to small-molecule substance under the effect of various enzyme in alimentary canal again.The abilities of digestive and absorption of animal feed Middle nutrition composition depends on the kind of enzyme in alimentary canal and active size.But the endogenous enzymes of the degraded feed ingredient of secreting in livestock and poultry alimentary tract often lacks and deficiency, as the phytase of phytic acid of degrading, cellulase and other some non-starch polysaccharide enzymes of degraded cellulose all compare shortage, this just greatly reduces the digestive utilization ratio of nutriment, thus need to add exogenous enzyme preparations in feed, to improve feed quality, improve efficiency of feed utilization.Along with the development of modern biotechnology; the cost of fodder enzyme preparation is more and more lower; but in use heat treatment; there is again very large impact to the stability of enzyme preparation in moisture and some metal ion etc.: phytase, amylase etc. can obviously reduce in the expanded middle activity of granulation; the activity of dry enzyme preparation is better; generally can tolerate 90 DEG C of high temperature 30 minutes and can not passivation inactivation; but the same temperature of the damp and hot generation of steam but can cause enzyme preparation fast deactivation; when granulator refining temperature reaches 75 DEG C, the activity of beta glucan only has 30% of initial activity.
Probiotics is a kind of prebiotics feed additive by improving gut flora balance, animal being applied to the work of Beneficial Effect, is active bacteria formulation that can be directly feeding, significant for raising livestock and poultry production performance.The current domestic confirmed bacterial classification being suitable for doing probio has 12 kinds, Bacillus acidi lactici, streptococcus, bacillus, Bifidobacterium and saccharomycete etc.Probiotics as feed addictive problem demanding prompt solution is: 1. active bacteria formulation easily loses biologically active in feed manufacturing, transport, storage process, these microorganisms are especially responsive to high temperature, and when pelleting temperature is more than 85 DEG C, activity almost loses Tai to the greatest extent; 2. after active bacteria formulation enters alimentary canal, the hydrochloric acid of low pH can not be stood, the effect of bile acid, be difficult to have enough number of viable to arrive enteron aisle or surely grow playing a role in enteron aisle that (General Requirements probiotics viable bacteria concentration is 10
7cfu/mL); 3. after active bacteria formulation enters enteron aisle, the speed of growth is slow, be difficult to have the advantage status in microorganism competition, form dominant microflora, therefore, although some microorganism formulation has good effect in laboratory conditions, be often difficult under production conditions get a desired effect.
For the feature of above-mentioned biology enzyme and probiotics, people adopt microcapsular envelopes technology that biology enzyme and probiotics are converted into stable fine-powdered particle, make it have good mobility and dispersiveness, be easy to mix with other feeds, and convenient transport, storage and interpolation; Through the product of coating due to the protection of membrane material, acidproof and resistance to elevated temperatures significantly improves.
At present, method for coating enzyme preparation (and probiotics) is a lot, spray drying process and spray cooling are conventional means, as Liu Hanling adopts beta-schardinger dextrin-to be wall material, the papain microcapsules that spray-dried method is obtained, after measured, microcapsule formulation heat endurance 75 DEG C time improves more than 2 times, and in feed steam pelletization, the activity of 1,4 beta-glucanase can retain more than 90%; Yuan Jieli etc. utilize Arabic gum to carry out coating to Bifidobacterium, make the Bifidobacterium death rate reduce 20% ~ 30%.But it is higher that spray-dired shortcoming is hot blast temperature, in processing, loss of activity is large, processing cost is also high simultaneously, prepare microcapsules by chemical method can carry out at a lower temperature, as carried out coating with sodium alginate and calcium chloride to peptostreptococcus and Bacillus acidi lactici, the significant prolongation survival period of Bacillus acidi lactici, but preparation feedback carries out in aqueous, and product drying needs higher temperature equally; Because said method is all make in air atmosphere, the oxygen in air can cause the death of anaerobe (as Bifidobacterium).
Summary of the invention
The object of the present invention is to provide that a kind of preparation method is simple, loss of activity is few and have biology enzyme or the probiotics of significantly antiacid, resistant to elevated temperatures fatty coating in process.
For achieving the above object, the present invention can take following technical proposals:
The biology enzyme of fatty coating of the present invention or probiotics are prepared from by following step:
The first step, to be solvent liquefaction oil gas (LPG), normal butane, high-purity isobutane (R600a), propane, the dimethyl ether (DME), 1 of gaseous state under normal temperature and pressure, 1,1,2-HFC-134a or sulfur hexafluoride through compressor boil down to fluid storage in storage tank;
Second step, coating material is placed in the dissolution kettle of jacketed, being evacuated to gauge pressure is 0.09MPa, enters in dissolution kettle by the liquid flux hydrocarbon infusion in above-mentioned storage tank, in chuck, logical hot water makes liquid flux heat up becomes subcritical fluids, stirs and makes coating material dissolves;
3rd step, enzyme preparation or probiotics is added in the encapsulating still of jacketed, being evacuated to gauge pressure after sealing is 0.09MPa, drive hydrocarbon pump and the solution containing coating material in dissolution kettle is injected encapsulating still, stir 20 ~ 30 minutes, decompression release solvent, phase transformation institute calorific requirement provides by encapsulating hot water in still chuck, hot water temperature is 0 ~ 80 DEG C, the material temperature controlled in encapsulating still is no more than 35 DEG C, when in encapsulating still, pressure is to normal pressure, being evacuated to gauge pressure is 0.09MPa, then emptying to make in encapsulating still pressure to normal pressure, discharging, namely coating enzyme preparation or probiotics finished product is obtained,
4th step, collect the gas-solvent discharged in the 3rd step, this gas-solvent is again converted into fluid storage and enters storage tank after purified treatment.
Above-mentioned 3rd step also can be replaced:
In the encapsulating still of jacketed, add enzyme preparation or probiotics, being evacuated to gauge pressure after sealing is 0.09MPa, drives hydrocarbon pump and the solution containing coating material in dissolution kettle is injected encapsulating still, stir 20 ~ 30 minutes; Material in encapsulating still is introduced spray tower through high-pressure pump, sprayed by the atomizer of spray tower, fluid solvent to gasify immediately release after atomizer decompression, and coating material adheres to the probiotics finished product of enzyme preparation or probiotics surface formation coating enzyme preparation or coating.Namely coordinate mist projection granulating, then subcritical fluids solvent can gasify through shower nozzle ejection in step-down immediately, and a large amount of heat energy of simultaneously stability, makes coating enzyme preparation and probiotics cool rapidly, reduce the height of spray tower, saves energy, and particle is more evenly fine and smooth simultaneously.
Described coating material comprises the mixture of hydrogenated vegetable oil or palm stearin or higher fatty acids polyol polyester or above-mentioned material.
Because above-mentioned coating material is hard fat, for helping fat to dissolve in enteron aisle, coating material can also comprise the glycerin monostearate of 1 ~ 20% or/and polyglycerol fatty acid ester is or/and stearic acid calcium lactate.
The granularity of described coating enzyme preparation or probiotics finished product is 0.02 ~ 0.5mm.
In second step, the mixing temperature of dissolution kettle is 0 ~ 35 DEG C (optimum temperature is 10 ~ 30 DEG C), and pressure is 0.1 ~ 0.8MPa.
The invention has the advantages that solution coating material subcritical fluids be dissolved into containing coating material, by solvent dilution coating material, then mix with enzyme preparation and probiotics and carry out coating, its advantage may be embodied in the following aspects: 1. whole process can complete under close to the condition of room temperature, greatly reduce enzyme preparation and probiotics inactivation in process, improve the activity of product.2. process is carried out under anaerobic, is especially applicable to the coating of the anaerobic bacterias such as Bifidobacterium.3. coating material adopts the composite of hard fat and polyester, can the process of wet-hot steam in resistance to feed manufacturing.4. membrane wrapping thickness is adjustable, and product can evenly discharge in enteron aisle.
Accompanying drawing explanation
Fig. 1 is the process chart of embodiment 1.
Fig. 2 is the process chart of embodiment 2,3.
Detailed description of the invention
The biology enzyme of fatty coating or the preparation method of probiotics is further illustrated below by specific embodiment.
Embodiment 1:
Coating material adopts glycerol stearate succinic acid polyester (fusing point 89 DEG C) 60%, and hydrogenated vegetable oil (fusing point 50 DEG C) 30%, the percentage by weight of polyglycerol fatty acid ester 10% is prepared.
As shown in Figure 1, agitator or supersonic generator is contained at the dissolution kettle 1(of jacketed, pressure-bearing P >=2MPa) in add the coating material that 350kg prepares according to the above ratio, sealing, to be evacuated to dissolution kettle vacuum meter registration be gauge pressure is 0.09MPa, drives hydrocarbon pump 3 and injects 2000L liquefied petroleum gas (or other solvents) (compress in advance, be cooled to liquid then be stored in storage tank 4), stir, logical hot water hydrotropy in chuck, contains the solution temperature of coating material lower than 35 DEG C in dissolution kettle 1.
By 150kg cellulase, (enzyme lives 1 × 10
4u/g) encapsulating still (adopting rake type dryer 2) is placed in, the solution being dissolved with coating material in dissolution kettle 1 to be squeezed in rake type dryer 2 mixing 30 minutes by hydrocarbon pump 5, open air valve 6 reduces pressure desolventizing (become liquid return in storage tank 4 for subsequent use through compressor 7, condenser 8 after the gas-solvent purification of release), material is released from drain hole and is collected, obtain the coating cellulase finished product of 30%, envelop rate 96.5%.When coated product makees pellet, through 80 DEG C of humid heat treatment, 20 minutes non-inactivations.Enter after in animal body as feed, the hydrochloric acid of low pH, the effect of bile acid can be tolerated, ensure that the vigor of cellulase plays a role in enteron aisle.
Embodiment 2:
Coating material adopts palm stearin 50%, and glycerol stearate adipate polyester 40%, the percentage by weight of polyglycerol fatty acid ester 10% is prepared.
As shown in Figure 2, agitator or supersonic generator is contained at dissolution kettle 1(, pressure-bearing P >=2MPa) in add the coating material that 200kg prepares according to the above ratio, sealing, to be evacuated to dissolution kettle vacuum meter registration be gauge pressure is 0.09MPa, drives hydrocarbon pump 3 and injects 1000L butane (or other solvents) (compress in advance, be cooled to liquid then be stored in storage tank 4), stir, chuck leads to hot water hydrotropy, and in dissolution kettle 1, solution temperature is lower than 35 DEG C, and in still to be dissolved, feed liquid becomes the supply of transparent rear stopping hot water.
In encapsulating still 2, adding 300kg lipase, (enzyme lives 1 × 10
5u/g), being evacuated to encapsulating still 2 vacuum meter registration is 0.09MPa, drives hydrocarbon pump 5 and the feed liquid in dissolution kettle 1 is squeezed in encapsulating still 2, be uniformly mixed (30 minutes); Drive high-pressure pump 6 and will encapsulate material introducing spray tower 7 in still 2, sprayed by the atomizer 8 of spray tower 7, fluid solvent butane to gasify immediately release after atomizer 8 reduces pressure, open air valve 9, become liquid through compressor 10, condenser 11 after the gas-solvent purification of release to return in storage tank 4 and reuse, collect the coating lipase finished product of content 60%.Envelop rate 92.1%, particle diameter 0.1 ~ 0.4mm, circular, good fluidity.When coated product makees pellet, through 80 DEG C of humid heat treatment, 20 minutes non-inactivations.Enter after in animal body as feed, the hydrochloric acid of low pH, the effect of bile acid can be tolerated, ensure that the vigor of lipase plays a role in enteron aisle.
Embodiment 3:
Coating material adopts hydrogenated vegetable oil 80%, and single stearic acid glycerine lipoprotein 10%, the percentage by weight of sucrose ester 10% is prepared.
As shown in Figure 2, agitator or supersonic generator is contained at dissolution kettle 1(, pressure-bearing P >=2MPa) in add the coating material that 100kg prepares by aforementioned proportion, sealing, to be evacuated to dissolution kettle vacuum meter registration be gauge pressure is 0.09MPa, drive hydrocarbon pump 3 and inject 1000L butane (or other solvents) solution (compress in advance, be cooled to liquid then be stored in storage tank 4), stir, chuck leads to hot water hydrotropy, in dissolution kettle 1 containing the solution temperature of coating material lower than 35 DEG C, until feed liquid in still become transparent after stop hot water supply.
400kg Bifidobacterium-lactic acid bacteria solid powder (number of viable 2 × 10 is added in encapsulating still 2
8individual/g), to be evacuated to encapsulating still vacuum meter registration be gauge pressure is 0.09MPa, drives hydrocarbon pump 5 and the feed liquid in dissolution kettle 1 is squeezed in encapsulating still 2, be uniformly mixed (30 minutes).Drive high-pressure pump 6 and will encapsulate material introducing spray tower 7 in still 2, sprayed by the atomizer 8 of spray tower 7, fluid solvent butane to gasify immediately release after atomizer decompression, open air valve 9, become liquid through compressor 10, condenser 11 after the purification of the gas-solvent of release and return in storage tank 4 and reuse; Collect the coating Bifidobacterium finished product of content 80%.Envelop rate 91.5%, particle diameter 0.1 ~ 0.4mm, circular, good fluidity.Coated product, 80 DEG C of humid heat treatment, processes 20 minutes, and number of viable preserves more than 90%.
Claims (6)
1. the biology enzyme of fatty coating or a probiotics, be is characterized in that: be prepared from by following step:
The first step, by under normal temperature and pressure be the solvent liquefaction oil gas of gaseous state, normal butane, high-purity isobutane, propane, dimethyl ether, HFA 134a or sulfur hexafluoride through compressor boil down to fluid storage in storage tank;
Second step, coating material is placed in the dissolution kettle of jacketed, being evacuated to gauge pressure is 0.09MPa, enters in dissolution kettle by the liquid flux hydrocarbon infusion in above-mentioned storage tank, in chuck, logical hot water makes liquid flux heat up becomes subcritical fluids, stirs and makes coating material dissolves;
3rd step, solid polypeptide formulation or probiotics is added in the encapsulating still of jacketed, being evacuated to gauge pressure after sealing is 0.09MPa, drive hydrocarbon pump and the solution containing coating material in dissolution kettle is injected encapsulating still, stir 20 ~ 30 minutes, decompression release solvent, phase transformation institute calorific requirement provides by encapsulating hot water in still chuck, hot water temperature is 0 ~ 80 DEG C, control material temperature in encapsulating still and be no more than 35 DEG C, when in encapsulating still, pressure is close to normal pressure, being evacuated to gauge pressure is 0.09MPa, then emptying to make in encapsulating still pressure to normal pressure, discharging, namely coating enzyme preparation or probiotics finished product is obtained,
4th step, collects the gas-solvent discharged in the 3rd step, and this gas-solvent is again converted into fluid storage through compression cooling and enters storage tank after purified treatment.
2. the biology enzyme of fatty coating according to claim 1 or probiotics, is characterized in that: above-mentioned 3rd step replaces with:
In the encapsulating still that jacketed can stir, add enzyme preparation or probiotics, being evacuated to gauge pressure after sealing is 0.09MPa, drives hydrocarbon pump and the solution containing coating material in dissolution kettle is injected encapsulating still, stir 20 ~ 30 minutes; Material in encapsulating still is introduced spray tower through high-pressure pump, sprayed by the atomizer of spray tower, fluid solvent to gasify immediately release after atomizer decompression, and coating material adheres to enzyme preparation or probiotics surface formation coating enzyme preparation or probiotics finished product.
3. the biology enzyme of fatty coating according to claim 1 or probiotics, is characterized in that: described coating material comprises the mixture of hydrogenated vegetable oil or palm stearin or higher fatty acids polyol polyester or above-mentioned material.
4. the biology enzyme of fatty coating according to claim 3 or probiotics, is characterized in that: described coating material also comprises the glycerin monostearate of 1 ~ 20% or/and sucrose ester is or/and polyglycerol fatty acid ester is or/and stearic acid calcium lactate.
5. the biology enzyme of fatty coating according to claim 1 or probiotics, is characterized in that: the granularity of described coating enzyme preparation or probiotics finished product is 0.02 ~ 0.5mm.
6. the biology enzyme of fatty coating according to claim 1 or probiotics, it is characterized in that: in described second step, the mixing temperature of dissolution kettle is 0 ~ 35 DEG C, pressure is 0.1 ~ 0.8MPa.
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| JP2562624B2 (en) * | 1986-11-07 | 1996-12-11 | 昭和電工株式会社 | Water-soluble microcapsule and liquid detergent composition |
| JP2598674B2 (en) * | 1988-05-06 | 1997-04-09 | 昭和電工株式会社 | Method for producing water-soluble microcapsules containing enzymes |
| GB9800936D0 (en) * | 1997-05-10 | 1998-03-11 | Univ Nottingham | Biofunctional polymers |
| JP2004195307A (en) * | 2002-12-17 | 2004-07-15 | Itec Co Ltd | Method and apparatus for producing microparticle or microcapsule by using high-pressure fluid |
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