CN101810253A - High temperature resistance diaphragm-controlled phytase pellets and preparation method thereof - Google Patents
High temperature resistance diaphragm-controlled phytase pellets and preparation method thereof Download PDFInfo
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- CN101810253A CN101810253A CN 201010136119 CN201010136119A CN101810253A CN 101810253 A CN101810253 A CN 101810253A CN 201010136119 CN201010136119 CN 201010136119 CN 201010136119 A CN201010136119 A CN 201010136119A CN 101810253 A CN101810253 A CN 101810253A
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- 108010011619 6-Phytase Proteins 0.000 title claims abstract description 110
- 229940085127 phytase Drugs 0.000 title claims abstract description 109
- 239000008188 pellet Substances 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title claims abstract description 42
- 238000000576 coating method Methods 0.000 claims abstract description 89
- 239000000463 material Substances 0.000 claims abstract description 62
- 239000011248 coating agent Substances 0.000 claims abstract description 26
- 230000000694 effects Effects 0.000 claims abstract description 10
- 239000012530 fluid Substances 0.000 claims description 35
- 239000011122 softwood Substances 0.000 claims description 30
- 238000004078 waterproofing Methods 0.000 claims description 27
- 238000003756 stirring Methods 0.000 claims description 22
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 17
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 16
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 16
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 16
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 16
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 16
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 14
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 14
- 239000003381 stabilizer Substances 0.000 claims description 13
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 12
- 235000002639 sodium chloride Nutrition 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 11
- 238000005243 fluidization Methods 0.000 claims description 11
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 11
- 238000007873 sieving Methods 0.000 claims description 11
- 239000011780 sodium chloride Substances 0.000 claims description 9
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 8
- 229920002261 Corn starch Polymers 0.000 claims description 8
- 229920002472 Starch Polymers 0.000 claims description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 8
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 8
- 239000001110 calcium chloride Substances 0.000 claims description 8
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 8
- 239000008120 corn starch Substances 0.000 claims description 8
- 229940099112 cornstarch Drugs 0.000 claims description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 8
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 8
- 239000001103 potassium chloride Substances 0.000 claims description 8
- 235000011164 potassium chloride Nutrition 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000000741 silica gel Substances 0.000 claims description 8
- 229910002027 silica gel Inorganic materials 0.000 claims description 8
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 8
- 235000017550 sodium carbonate Nutrition 0.000 claims description 8
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 8
- 235000011152 sodium sulphate Nutrition 0.000 claims description 8
- 239000008107 starch Substances 0.000 claims description 8
- 235000019698 starch Nutrition 0.000 claims description 8
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 7
- 235000010489 acacia gum Nutrition 0.000 claims description 7
- 239000001785 acacia senegal l. willd gum Substances 0.000 claims description 7
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims description 7
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims description 7
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims description 7
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 7
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 7
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 7
- 229920001285 xanthan gum Polymers 0.000 claims description 7
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 claims description 6
- 239000011734 sodium Substances 0.000 claims description 6
- 229910052708 sodium Inorganic materials 0.000 claims description 6
- 239000004925 Acrylic resin Substances 0.000 claims description 5
- 239000001856 Ethyl cellulose Substances 0.000 claims description 5
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 claims description 5
- 229920002774 Maltodextrin Polymers 0.000 claims description 5
- 239000005913 Maltodextrin Substances 0.000 claims description 5
- 235000019325 ethyl cellulose Nutrition 0.000 claims description 5
- 229920001249 ethyl cellulose Polymers 0.000 claims description 5
- 229940035034 maltodextrin Drugs 0.000 claims description 5
- 229920001223 polyethylene glycol Polymers 0.000 claims description 5
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 4
- 239000002202 Polyethylene glycol Substances 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 229960003943 hypromellose Drugs 0.000 claims description 4
- 239000008101 lactose Substances 0.000 claims description 4
- 235000009973 maize Nutrition 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims description 4
- XNGIFLGASWRNHJ-UHFFFAOYSA-L phthalate(2-) Chemical compound [O-]C(=O)C1=CC=CC=C1C([O-])=O XNGIFLGASWRNHJ-UHFFFAOYSA-L 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 229960001763 zinc sulfate Drugs 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims description 3
- 229920000623 Cellulose acetate phthalate Polymers 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 claims description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 3
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims description 3
- 229940081734 cellulose acetate phthalate Drugs 0.000 claims description 3
- 235000009508 confectionery Nutrition 0.000 claims description 3
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims description 3
- 229940031703 low substituted hydroxypropyl cellulose Drugs 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 235000013809 polyvinylpolypyrrolidone Nutrition 0.000 claims description 3
- 229920000523 polyvinylpolypyrrolidone Polymers 0.000 claims description 3
- 229940072033 potash Drugs 0.000 claims description 3
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 3
- 235000015320 potassium carbonate Nutrition 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 235000015424 sodium Nutrition 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 abstract description 20
- 102000004190 Enzymes Human genes 0.000 abstract description 20
- 229940088598 enzyme Drugs 0.000 abstract description 20
- 238000000034 method Methods 0.000 abstract description 8
- 230000008569 process Effects 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 18
- 239000002245 particle Substances 0.000 description 11
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 10
- 239000011574 phosphorus Substances 0.000 description 10
- 229910052698 phosphorus Inorganic materials 0.000 description 10
- 239000006187 pill Substances 0.000 description 9
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 9
- 229920000053 polysorbate 80 Polymers 0.000 description 9
- 238000005507 spraying Methods 0.000 description 9
- 239000007864 aqueous solution Substances 0.000 description 8
- 238000010438 heat treatment Methods 0.000 description 8
- 229920000642 polymer Polymers 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000005303 weighing Methods 0.000 description 8
- DOOTYTYQINUNNV-UHFFFAOYSA-N Triethyl citrate Chemical compound CCOC(=O)CC(O)(C(=O)OCC)CC(=O)OCC DOOTYTYQINUNNV-UHFFFAOYSA-N 0.000 description 5
- 239000001069 triethyl citrate Substances 0.000 description 5
- VMYFZRTXGLUXMZ-UHFFFAOYSA-N triethyl citrate Natural products CCOC(=O)C(O)(C(=O)OCC)C(=O)OCC VMYFZRTXGLUXMZ-UHFFFAOYSA-N 0.000 description 5
- 235000013769 triethyl citrate Nutrition 0.000 description 5
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- URAYPUMNDPQOKB-UHFFFAOYSA-N triacetin Chemical compound CC(=O)OCC(OC(C)=O)COC(C)=O URAYPUMNDPQOKB-UHFFFAOYSA-N 0.000 description 4
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 3
- 235000002949 phytic acid Nutrition 0.000 description 3
- 239000004014 plasticizer Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- PYGXAGIECVVIOZ-UHFFFAOYSA-N Dibutyl decanedioate Chemical compound CCCCOC(=O)CCCCCCCCC(=O)OCCCC PYGXAGIECVVIOZ-UHFFFAOYSA-N 0.000 description 2
- 229920003151 Eudragit® RL polymer Polymers 0.000 description 2
- 229920003166 Eudragit® RL/RS polymer Polymers 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- DNKKLDKIFMDAPT-UHFFFAOYSA-N n,n-dimethylmethanamine;2-methylprop-2-enoic acid Chemical compound CN(C)C.CC(=C)C(O)=O.CC(=C)C(O)=O DNKKLDKIFMDAPT-UHFFFAOYSA-N 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- -1 antiplastering aid Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000012407 engineering method Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 229940084936 gonak Drugs 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000005563 spheronization Methods 0.000 description 1
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a high temperature resistance diaphragm-controlled phytase pellets and a preparation method thereof. The high temperature resistance diaphragm-controlled phytase pellet is composed of phytase pellets and coating materials which are coated on the outer layer and has high temperature resistance action, and the weight ratio is 20-80:80-20. The diaphragm-controlled phytase pellets prepared by the coating method has favorable liquidity and dispersibility and strong capability of resisting damp and heat, avoids enzyme activity loss in the feed processing, transportation and storage processes, and effectively improves the phytase heat stability and storage stability.
Description
Technical field
The present invention relates to a kind of preparation method of feed addictive, relate in particular to a kind of preparation method of high temperature resistance diaphragm-controlled phytase pellets.
Background technology
Mineral matter phosphorus is growth of animal and produces one of essential nutrient, is the important constituent of body, again the many important physical of wide participation body, biochemical process.Domestic animal must obtain sufficient phosphorus from feed, just can keep normal life and activity in production.The plant feed proportion is very big in the monogastric animal feed, wherein 60～80% phosphorus is that form with phytate phosphorus exists, the abundant digestibility and utilization phosphorus wherein because nonruminant lack to decompose the required enzyme of phytate phosphorus, therefore must in feed, add the expensive inorganic phosphate of price, this has not only improved feed cost, and the phosphorus that can not digest and assimilate discharges with ight soil, causes environmental pollution.
The interpolation phytase can discharge the phosphorus in the feed, and phosphorus can be utilized by nonruminant, eliminates the anti-oxidant action of phytic acid; Reduce the drainage of phosphorus in environment, reduced agroecological burden, and slowed down the eutrophication of aquatic environment; Simultaneously, also reduced the addition of Phos in the feed.To improving the breeding performonce fo animals and the extremely important meaning of having preserved the ecological environment.
Yet resistance, the especially heat endurance of some present phytase products can not satisfy the requirement of feed and feed processing, become one of critical limitation sexual factor that the restriction phytase applies.The method that improves feeding enzyme heat stability is a lot, and for phytase, method commonly used has following several: (1) natural selection method, screen the phytase that high temperature resistant bacterial classification discharges.(2) immobilization embedded: as enzyme is fixed on the insoluble carrier, thereby the stabilized enzyme conformation to be eliminated the metamorphosis of contrary sex factor, thereby improve phytase stability.(3) protein engineering method: promptly obtain the gene of high temperature resistant enzyme, express obtaining thermostable phytases again by the genetic engineering reorganization.(4) chemical modification method: by certain or certain amino acid at chemical reagent specificity modification enzyme activity center, thereby the conformation of stable protein improves its hear resistance.(5) galenic pharmacy method: by adding stabilizing agent to phytase solution, phytase combine with carrier make plain particles, spray, wrap the heat endurance that galenic pharmacy technology such as quilt improves phytase after the phytase particle is carried out liquid.
Therefore, need research to improve the method for phytase heat endurance, the resistance of raising phytase really reduces the phytase loss that enzyme is lived in the feed granulating process effectively.
Summary of the invention
The objective of the invention is provides a kind of high temperature resistance diaphragm-controlled phytase pellets and preparation method thereof at the deficiencies in the prior art.The present invention can tolerate the high temperature (more than 85 ℃) of granulating and pushing and keep higher activity in the feed process, avoid losing efficacy, and keeps enzyme to greatest extent and lives, the effect of performance enzyme.
The objective of the invention is to be achieved through the following technical solutions:
A kind of high temperature resistance diaphragm-controlled phytase pellets, it mainly is made up of phytase ball core and coatings.Phytase ball core is 20～80: 80～20 with being coated on outer field weight proportion with coatings of high temperature resistant effect.Be preferably 50～80: 50～20.
Further, described coatings is made up of three parts, comprises the water-soluble suction section bar bed of material, water-soluble thermostable type material layer and fat-soluble waterproofing type material layer from inside to outside successively.Coatings weight proportion from inside to outside is 20～60: 10～50: 30～60, be preferably 30～50: 10～30: 40～60.
Further, described phytase ball core is to extrude round as a ball making after being mixed by phytase zymotic fluid, auxiliary material, stabilizing agent and antiplastering aid.Wherein, auxiliary material can select for use in starch, cornstarch, maize cob meal, maltodextrin, lactose, white carbon, microcrystalline cellulose, sweet mellow wine, inorganic salts, polyethylene glycol, PVP, PVPP, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, crosslinked carboxymethyl fecula sodium, the lauryl sodium sulfate one or more to make.Stabilizing agent is a kind of or multiple mixing arbitrarily in ammonium sulfate, magnesium sulfate, sodium sulphate, sodium carbonate, potassium chloride, sodium chloride, the calcium chloride, and antiplastering aid can be a kind of or multiple mixing arbitrarily in talcum powder, superfine silica gel powder, the dolomol.The weight proportion of phytase zymotic fluid, auxiliary material, stabilizing agent and antiplastering aid is 1: 1～1.15: 0.1～0.225: 0.1～0.338.
Further, in the described coatings, the water-soluble suction section bar bed of material comprise in sodium chloride, sodium carbonate, potash, magnesium sulfate, ammonium sulfate, zinc sulfate, sodium sulphate, potassium chloride, the calcium chloride any one or multiple; Water-soluble thermostable type material layer is xanthans or Arabic gum; Fat-soluble waterproofing type material layer is one or more in polyacrylic resin, Cellulose Acetate Phthalate, ethyl cellulose, hydroxypropyl methylcellulose, hypromellose phthalate, the polyvinylpyrrolidone.
The preparation method of above-mentioned high temperature resistance diaphragm-controlled phytase pellets may further comprise the steps:
(1) under stirring state, stabilizing agent and antiplastering aid are slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again the auxiliary material mixing of sieving;
(2) softwood of making is placed extrude spheronizator and extrude and round as a ball, make phytase ball core;
(3) dispose three layers of coating solution respectively, promptly under stirring condition, coating material is slowly added in the entry, mix.Get an amount of phytase pellets and place fluid bed, evenly spray, treat to make after three layers of dressing finish micropill fluidisation 20～40 minutes in fluid bed;
(4) drying places the phytase pellets behind the dressing 35～55 ℃ dry 6～12 hours of air dry oven.
The invention has the beneficial effects as follows: high temperature resistance diaphragm-controlled phytase pellets flowability, the good dispersion of the present invention preparation can tolerate the damp and hot effect of strong high temperature and keep very high enzymatic activity.Through simulation feed processing experiment, 90 ℃ of enzymes after 2 minutes are lived retention rate more than 90%.Compare with the phytase plain particles, its enzyme retention rate alive has improved 52%.
The specific embodiment
High temperature resistance diaphragm-controlled phytase pellets of the present invention is made by phytase ball core and suitable high temperature resistant coating material.Phytase ball core is 20～80: 80～20 with being coated on outer field weight proportion with coating material of high temperature resistant effect, and preferred weight proportion is 50～80: 50～20.
The coatings of high temperature resistance diaphragm-controlled dose of phytase pellets is made up of three parts, comprises the water-soluble suction section bar bed of material, water-soluble thermostable type material layer and fat-soluble waterproofing type material layer from inside to outside successively.Coatings weight proportion from inside to outside is 20～60: 10～50: 30～60, be preferably 30～50: 10～30: 40～60.
Phytase ball core is to extrude round as a ball making after being mixed by phytase zymotic fluid, auxiliary material, stabilizing agent and antiplastering aid.Wherein, auxiliary material can select for use in starch, cornstarch, maize cob meal, maltodextrin, lactose, white carbon, microcrystalline cellulose, sweet mellow wine, inorganic salts, polyethylene glycol, PVP, PVPP, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, crosslinked carboxymethyl fecula sodium, the lauryl sodium sulfate one or more to make.Stabilizing agent is a kind of or multiple mixing arbitrarily in ammonium sulfate, magnesium sulfate, sodium sulphate, sodium carbonate, potassium chloride, sodium chloride, the calcium chloride, and antiplastering aid can be a kind of or multiple mixing arbitrarily in talcum powder, superfine silica gel powder, the dolomol.
The weight proportion of phytase zymotic fluid, auxiliary material, stabilizing agent and antiplastering aid is 1: 1～1.15: 0.1～0.225: 0.1～0.338.
In the coatings, the water-soluble suction section bar bed of material comprise in sodium chloride, sodium carbonate, potash, magnesium sulfate, ammonium sulfate, zinc sulfate, sodium sulphate, potassium chloride, the calcium chloride any one or multiple; Water-soluble thermostable type material layer is one or more in xanthans, the Arabic gum; Fat-soluble waterproofing type material layer is one or more in polyacrylic resin, Cellulose Acetate Phthalate, ethyl cellulose, hydroxypropyl methylcellulose, hypromellose phthalate, the polyvinylpyrrolidone.
In addition, all can add plasticizer, antiplastering aid, surfactant as one sees fit in the fat-soluble waterproofing type material layer.Plasticizer is one or more in polyethylene glycols, triethyl citrate, dibutyl sebacate, repefral, the triacetyl glycerine; Antiplastering aid is one or more in talcum powder, dolomol, superfine silica gel powder, the glycerin monostearate; Surfactant is a Tween-80.The content of plasticizer is the 0.1%-10% of fat-soluble waterproofing type material layer dry weight.The content of antiplastering aid is the 0.1%-10% of fat-soluble waterproofing type material layer dry weight.Surface-active contents is the 0.1%-10% of fat-soluble waterproofing type material layer dry weight.
The preparation method of high temperature resistance diaphragm-controlled phytase pellets of the present invention,, employing is earlier extruded spheronization and is prepared phytase ball core, uses the bottom spraying type fluid bed again phytase pellets is prepared into coating micro-pill.Comprise:
Step 1: under stirring state, stabilizing agent and antiplastering aid are slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again with the auxiliary material mixing of sieving;
Step 2: the softwood of making placed extrude spheronizator and extrude and round as a ball, make phytase ball core;
Step 3: dispose three layers of coating solution respectively, promptly under stirring condition, coating material is slowly added in the entry, mix.Get an amount of phytase pellets and place fluid bed, evenly spray, treat to make after three layers of dressing finish micropill fluidisation 20～40 minutes in fluid bed;
Step 4: drying places the phytase pellets behind the dressing 35～55 ℃ dry 6～12 hours of air dry oven.
Describe the present invention in detail according to embodiment below, it is more obvious that purpose of the present invention and effect will become.
Embodiment 1:
The phytase pellets preparation
Softwood preparation: under stirring state, 5% sodium sulphate is slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again the mixing of sieving of auxiliary material 100g cornstarch, 150g white carbon, standby.
Extrude round as a ball: place extruder to extrude softwood, regulate extruded velocity, treat the long strip type extrudate all fall into spheronizator after beginning round as a ball, regulate suitable round as a ball speed, the round as a ball time is 3-15 minute.
The preparation of phytase coating micro-pill
Water-soluble suction type coatings: 1% polyvinylpyrrolidone aqueous solution 50mL, wherein contain 30% potassium chloride, talcum powder is an amount of.
Water-soluble thermostable type coatings: xanthans 5g, water is configured to solution, and is standby.
Fat-soluble waterproofing type coatings: (Eudragit RL: EudragitRS=1: 4), wherein glycerin monostearate, Tween-80, triethyl citrate are respectively 5%, 2%, 20% of polymer dry weight to Eudragit RL/RS solution 45mL.
Preparation technology: take by weighing phytase pellets 50g, place the bottom spraying type fluidized-bed coating machine, configure water-soluble suction type coatings, stir, dressing, fluidisation is 20 minutes behind the dressing, same operation, go up water-soluble thermostable type coatings successively and fat-soluble waterproofing type coatings temperature of charge is controlled at below 37 ℃, after dressing finishes, place 45 ℃ of drying boxes oven dry 6 hours promptly again.
Testing result: products obtained therefrom roundness, flowability are all good, and 20-30 order particle yield reaches 88.6%, and the residual enzyme work after 100 ℃ of steam heat treatment reaches 87.4%.
Embodiment 2:
The phytase pellets preparation
Softwood preparation: under stirring state, 4% magnesium sulfate is slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again the mixing of sieving of auxiliary material 100g microcrystalline cellulose, 30g white carbon, 70g maltodextrin, standby.
Extrude round as a ball: place extruder to extrude softwood, regulate extruded velocity, treat the long strip type extrudate all fall into spheronizator after beginning round as a ball, regulate suitable round as a ball speed, the round as a ball time is 3-15 minute.
The preparation of phytase coating micro-pill
Water-soluble suction type coatings: 1% polyvinylpyrrolidone aqueous solution 40mL, wherein contain 15% zinc sulfate, superfine silica gel powder is an amount of.
Water-soluble thermostable type coatings: Arabic gum 6g, water is configured to solution, and is standby.
Fat-soluble waterproofing type coatings: ethyl cellulose 20mL, wherein dolomol, Tween-80, dibutyl sebacate are respectively 50%, 0.1%, 10% of polymer dry weight.
Preparation technology: take by weighing phytase pellets 50g, place the bottom spraying type fluidized-bed coating machine, configure water-soluble suction type coatings, stir, dressing, fluidisation is 20 minutes behind the dressing, same operation, go up water-soluble thermostable type coatings successively and fat-soluble waterproofing type coatings temperature of charge is controlled at below 37 ℃, after dressing finishes, place 45 ℃ of drying boxes oven dry 8 hours promptly again.
Testing result: products obtained therefrom roundness, flowability are all good, and 20-30 order particle yield reaches 86.2%, and the residual enzyme work after 100 ℃ of steam heat treatment reaches 82.4%.
Embodiment 3:
The phytase pellets preparation
Softwood preparation: under stirring state, 5% sodium chloride is slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again the mixing of sieving of auxiliary material 100g cornstarch, 50g lactose and 50g microcrystalline cellulose, standby.
Extrude round as a ball: place extruder to extrude softwood, regulate extruded velocity, treat the long strip type extrudate all fall into spheronizator after beginning round as a ball, regulate suitable round as a ball speed, the round as a ball time is 3-15 minute.
The preparation of phytase coating micro-pill
Water-soluble suction type coatings: 1% polyvinylpyrrolidone aqueous solution 50mL, wherein contain 15% ammonium sulfate, talcum powder is an amount of.
Water-soluble thermostable type coatings: Arabic gum 1.25g, water is configured to solution, and is standby.
Fat-soluble waterproofing type coatings: hypromellose phthalate solution 8.5mL, wherein superfine silica gel powder, Tween-80, repefral are respectively 20%, 7%, 50% of polymer dry weight.
Preparation technology: take by weighing phytase pellets 50g, place the bottom spraying type fluidized-bed coating machine, configure water-soluble suction type coatings, stir, dressing, fluidisation is 20 minutes behind the dressing, same operation, go up water-soluble thermostable type coatings successively and fat-soluble waterproofing type coatings temperature of charge is controlled at below 37 ℃, after dressing finishes, place 45 ℃ of drying boxes oven dry 7 hours promptly again.
Testing result: products obtained therefrom roundness, flowability are all good, and 20-30 order particle yield reaches 90.5%, and the residual enzyme work after 100 ℃ of steam heat treatment reaches 85%.
Embodiment 4:
The phytase pellets preparation
Softwood preparation: under stirring state, 5% sodium carbonate is slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again the mixing of sieving of auxiliary material 20g starch, 80g microcrystalline cellulose, 100g white carbon, standby.
Extrude round as a ball: place extruder to extrude softwood, regulate extruded velocity, treat the long strip type extrudate all fall into spheronizator after beginning round as a ball, regulate suitable round as a ball speed, the round as a ball time is 3-15 minute.
The preparation of phytase coating micro-pill
Water-soluble suction type coatings: 1% polyvinylpyrrolidone aqueous solution 20mL, wherein contain 20% sodium chloride, talcum powder is an amount of.
Water-soluble thermostable type coatings: xanthans 7.8g, water is configured to solution, and is standby.
Fat-soluble waterproofing type coatings: polyacrylic resin solution 52mL, wherein talcum powder, Tween-80, triethyl citrate are respectively 10%, 5%, 30% of polymer dry weight.
Preparation technology: take by weighing phytase pellets 50g, place the bottom spraying type fluidized-bed coating machine, configure water-soluble suction type coatings, stir, dressing, fluidisation is 20 minutes behind the dressing, same operation, go up water-soluble thermostable type coatings successively and fat-soluble waterproofing type coatings temperature of charge is controlled at below 37 ℃, after dressing finishes, place 45 ℃ of drying boxes oven dry 10 hours promptly again.
Testing result: products obtained therefrom roundness, flowability are all good, and 20-30 order particle yield reaches 83.9%, and the residual enzyme work after 100 ℃ of steam heat treatment reaches 85.8%.
Embodiment 5:
The phytase pellets preparation
Softwood preparation: under stirring state, 5% potassium chloride is slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again the mixing of sieving of auxiliary material 150g microcrystalline cellulose, 100g maize cob meal, standby.
Extrude round as a ball: place extruder to extrude softwood, regulate extruded velocity, treat the long strip type extrudate all fall into spheronizator after beginning round as a ball, regulate suitable round as a ball speed, the round as a ball time is 3-15 minute.
The preparation of phytase coating micro-pill
Water-soluble suction type coatings: 1% polyvinylpyrrolidone aqueous solution 50mL, wherein contain 20% magnesium sulfate, talcum powder is an amount of.
Water-soluble thermostable type coatings: Arabic gum 3g, water is configured to solution, and is standby.
Fat-soluble waterproofing type coatings: (Eudragit RL: EudragitRS=1: 4), wherein glycerin monostearate, Tween-80, triacetyl glycerine are respectively 30%, 6%, 35% of polymer dry weight to Eudragit RL/RS solution 60mL.
Preparation technology: take by weighing phytase pellets 50g, place the bottom spraying type fluidized-bed coating machine, configure water-soluble suction type coatings, stir, dressing, fluidisation is 20 minutes behind the dressing, same operation, go up water-soluble thermostable type coatings successively and fat-soluble waterproofing type coatings temperature of charge is controlled at below 37 ℃, after dressing finishes, place 45 ℃ of drying boxes oven dry 8 hours promptly again.
Testing result: products obtained therefrom roundness, flowability are all good, and 20-30 order particle yield reaches 84.4%, and the residual enzyme work after 100 ℃ of steam heat treatment reaches 86.9%.
Embodiment 6:
The phytase pellets preparation
Softwood preparation: under stirring state, 5% ammonium sulfate is slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again the mixing of sieving of auxiliary material 70g cornstarch, 100g cornstarch, 30g maltodextrin, standby.
Extrude round as a ball: place extruder to extrude softwood, regulate extruded velocity, treat the long strip type extrudate all fall into spheronizator after beginning round as a ball, regulate suitable round as a ball speed, the round as a ball time is 3-15 minute.
The preparation of phytase coating micro-pill
Water-soluble suction type coatings: 1% polyvinylpyrrolidone aqueous solution 100mL, wherein contain 16% sodium carbonate, talcum powder is an amount of.
Water-soluble thermostable type coatings: Arabic gum 8g, water is configured to solution, and is standby.
Fat-soluble waterproofing type coatings: Gonak 54mL, wherein dolomol, Tween-80, triethyl citrate are respectively 25%, 4%, 15% of polymer dry weight.
Preparation technology: take by weighing phytase pellets 50g, place the bottom spraying type fluidized-bed coating machine, configure water-soluble suction type coatings, stir, dressing, fluidisation is 20 minutes behind the dressing, same operation, go up water-soluble thermostable type coatings successively and fat-soluble waterproofing type coatings temperature of charge is controlled at below 37 ℃, after dressing finishes, place 45 ℃ of drying boxes oven dry 7 hours promptly again.
Testing result: products obtained therefrom roundness, flowability are all good, and 20-30 order particle yield reaches 82.5%, and the residual enzyme work after 100 ℃ of steam heat treatment reaches 84.6%.
Embodiment 7:
The phytase pellets preparation
Softwood preparation: under stirring state, 5% calcium chloride is slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again the mixing of sieving of auxiliary material 20g superfine silica gel powder, 80g white carbon and 100g starch, standby.
Extrude round as a ball: place extruder to extrude softwood, regulate extruded velocity, treat the long strip type extrudate all fall into spheronizator after beginning round as a ball, regulate suitable round as a ball speed, the round as a ball time is 3-15 minute.
The preparation of phytase coating micro-pill
Water-soluble suction type coatings: 1% polyvinylpyrrolidone aqueous solution 50mL, wherein contain 24% sodium chloride, talcum powder is an amount of.
Water-soluble thermostable type coatings: xanthans 6g, water is configured to solution, and is standby.
Fat-soluble waterproofing type coatings: polyacrylic resin solution 40mL, wherein glycerin monostearate, Tween-80, polyethylene glycol are respectively 40%, 2.5%, 25% of polymer dry weight.
Preparation technology: take by weighing phytase pellets 50g, place the bottom spraying type fluidized-bed coating machine, configure water-soluble suction type coatings, stir, dressing, fluidisation is 20 minutes behind the dressing, same operation, go up water-soluble thermostable type coatings successively and fat-soluble waterproofing type coatings temperature of charge is controlled at below 37 ℃, after dressing finishes, place 45 ℃ of drying boxes oven dry 6 hours promptly again.
Testing result: products obtained therefrom roundness, flowability are all good, and 20-30 order particle yield reaches 86.6%, and the residual enzyme work after 100 ℃ of steam heat treatment reaches 86.5%.
Embodiment 8:
The phytase pellets preparation
Softwood preparation: under stirring state, 5% sodium sulphate is slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again the mixing of sieving of auxiliary material 80g cornstarch, 120g white carbon, standby.
Extrude round as a ball: place extruder to extrude softwood, regulate extruded velocity, treat the long strip type extrudate all fall into spheronizator after beginning round as a ball, regulate suitable round as a ball speed, the round as a ball time is 3-15 minute.
The preparation of phytase coating micro-pill
Water-soluble suction type coatings: 1% polyvinylpyrrolidone aqueous solution 100mL, wherein contain 37% calcium chloride, talcum powder is an amount of.
Water-soluble thermostable type coatings: xanthans 9.4g, water is configured to solution, and is standby.
Fat-soluble waterproofing type coatings: ethyl cellulose solution 32mL, wherein superfine silica gel powder, Tween-80, triethyl citrate are respectively 8%, 3%, 10% of polymer dry weight.
Preparation technology: take by weighing phytase pellets 50g, place the bottom spraying type fluidized-bed coating machine, configure water-soluble suction type coatings, stir, dressing, fluidisation is 20 minutes behind the dressing, same operation, go up water-soluble thermostable type coatings successively and fat-soluble waterproofing type coatings temperature of charge is controlled at below 37 ℃, after dressing finishes, place 45 ℃ of drying boxes oven dry 12 hours promptly again.
Testing result: products obtained therefrom roundness, flowability are all good, and 20-30 order particle yield reaches 83.2%, and the residual enzyme work after 100 ℃ of steam heat treatment reaches 82.7%.
The foregoing description is used for the present invention that explains, rather than limits the invention, and in the protection domain of spirit of the present invention and claim, any modification and change to the present invention makes all fall into protection scope of the present invention.
Claims (7)
1. a high temperature resistance diaphragm-controlled phytase pellets is characterized in that, it mainly is made up of phytase ball core and coatings.Phytase ball core is 20～80: 80～20 with being coated on outer field weight proportion with coatings of high temperature resistant effect.
2. according to the described high temperature resistance diaphragm-controlled phytase pellets of claim 1, it is characterized in that the weight proportion of described phytase ball core and coatings is 50～80: 50～20.
3. according to the described high temperature resistance diaphragm-controlled phytase pellets of claim 1, it is characterized in that described coatings is made up of three parts, comprise the water-soluble suction section bar bed of material, water-soluble thermostable type material layer and fat-soluble waterproofing type material layer from inside to outside successively.Coatings weight proportion from inside to outside is 20～60: 10～50: 30～60.
4. according to the described high temperature resistance diaphragm-controlled phytase pellets of claim 3, it is characterized in that the weight proportion of the described water-soluble suction section bar bed of material, water-soluble thermostable type material layer and fat-soluble waterproofing type material layer is for being 30～50: 10～30: 40～60.
5. according to the described high temperature resistance diaphragm-controlled phytase pellets of claim 1, it is characterized in that described phytase ball core is to extrude round as a ball making after being mixed by phytase zymotic fluid, auxiliary material, stabilizing agent and antiplastering aid.Wherein, auxiliary material can select for use in starch, cornstarch, maize cob meal, maltodextrin, lactose, white carbon, microcrystalline cellulose, sweet mellow wine, inorganic salts, polyethylene glycol, PVP, PVPP, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, crosslinked carboxymethyl fecula sodium, the lauryl sodium sulfate one or more to make.Stabilizing agent is a kind of or multiple mixing arbitrarily in ammonium sulfate, magnesium sulfate, sodium sulphate, sodium carbonate, potassium chloride, sodium chloride, the calcium chloride, and antiplastering aid can be a kind of or multiple mixing arbitrarily in talcum powder, superfine silica gel powder, the dolomol.The weight proportion of phytase zymotic fluid, auxiliary material, stabilizing agent and antiplastering aid is 1: 1～1.15: 0.1～0.225: 0.1～0.338.
6. according to the described high temperature resistance diaphragm-controlled phytase pellets of claim 3, it is characterized in that, in the described coatings, the water-soluble suction section bar bed of material comprise in sodium chloride, sodium carbonate, potash, magnesium sulfate, ammonium sulfate, zinc sulfate, sodium sulphate, potassium chloride, the calcium chloride any one or multiple; Water-soluble thermostable type material layer is xanthans or Arabic gum; Fat-soluble waterproofing type material layer is one or more in polyacrylic resin, Cellulose Acetate Phthalate, ethyl cellulose, hydroxypropyl methylcellulose, hypromellose phthalate, the polyvinylpyrrolidone.
7. the preparation method of the described high temperature resistance diaphragm-controlled phytase pellets of claim 1 is characterized in that, may further comprise the steps:
(1) under stirring state, stabilizing agent and antiplastering aid are slowly added in the phytase zymotic fluid,, phytase zymotic fluid and auxiliary material are mixed make softwood again the auxiliary material mixing of sieving.
(2) softwood of making is placed extrude spheronizator and extrude and round as a ball, make phytase ball core.
(3) dispose three layers of coating solution respectively, promptly under stirring condition, coating material is slowly added in the entry, mix.Get an amount of phytase pellets and place fluid bed, evenly spray, treat to make after three layers of dressing finish micropill fluidisation 20～40 minutes in fluid bed.
(4) drying places the phytase pellets behind the dressing 35～55 ℃ dry 6～12 hours of air dry oven.
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Cited By (8)
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| CN102511665A (en) * | 2011-12-29 | 2012-06-27 | 青岛蔚蓝生物集团有限公司 | Phytase-coated pellets and preparation method thereof |
| CN102599334A (en) * | 2012-02-14 | 2012-07-25 | 广西南宁市圣农科技开发有限公司 | Chinese medicinal herb feed additive with functions of digestion assistance and disease prevention and cure for livestock and poultry and preparation method of Chinese medicinal herb feed additive |
| CN103555706A (en) * | 2013-10-17 | 2014-02-05 | 青岛蔚蓝生物集团有限公司 | Production method of coated enzyme granules |
| CN106173265A (en) * | 2016-07-13 | 2016-12-07 | 湖南晶天科技实业有限公司 | A kind of feed additive enteric coating formic acid and preparation method thereof and a kind of feedstuff |
| CN109527224A (en) * | 2018-12-30 | 2019-03-29 | 珠海天凯生物科技有限公司 | A kind of complex enzyme preparation for feeding |
| CN112656769A (en) * | 2021-01-20 | 2021-04-16 | 天津市中央药业有限公司 | Cefdinir dispersible tablet |
| CN115433380A (en) * | 2022-09-03 | 2022-12-06 | 东莞市零度导热材料有限公司 | Manufacturing process of low-volatility heat conducting fin |
| WO2023083097A1 (en) * | 2021-11-15 | 2023-05-19 | 天津博菲德科技有限公司 | Hot melt coating method for granular enzyme preparation and application thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102511665A (en) * | 2011-12-29 | 2012-06-27 | 青岛蔚蓝生物集团有限公司 | Phytase-coated pellets and preparation method thereof |
| CN102511665B (en) * | 2011-12-29 | 2013-06-05 | 青岛玛斯特生物技术有限公司 | Phytase-coated pellets and preparation method thereof |
| CN102599334A (en) * | 2012-02-14 | 2012-07-25 | 广西南宁市圣农科技开发有限公司 | Chinese medicinal herb feed additive with functions of digestion assistance and disease prevention and cure for livestock and poultry and preparation method of Chinese medicinal herb feed additive |
| CN102599334B (en) * | 2012-02-14 | 2014-03-26 | 广西南宁市圣农科技开发有限公司 | Chinese medicinal herb feed additive with functions of digestion assistance and disease prevention and cure for livestock and poultry and preparation method of Chinese medicinal herb feed additive |
| CN103555706A (en) * | 2013-10-17 | 2014-02-05 | 青岛蔚蓝生物集团有限公司 | Production method of coated enzyme granules |
| CN106173265A (en) * | 2016-07-13 | 2016-12-07 | 湖南晶天科技实业有限公司 | A kind of feed additive enteric coating formic acid and preparation method thereof and a kind of feedstuff |
| CN109527224A (en) * | 2018-12-30 | 2019-03-29 | 珠海天凯生物科技有限公司 | A kind of complex enzyme preparation for feeding |
| CN112656769A (en) * | 2021-01-20 | 2021-04-16 | 天津市中央药业有限公司 | Cefdinir dispersible tablet |
| CN112656769B (en) * | 2021-01-20 | 2022-08-23 | 天津市中央药业有限公司 | Cefdinir dispersible tablet |
| WO2023083097A1 (en) * | 2021-11-15 | 2023-05-19 | 天津博菲德科技有限公司 | Hot melt coating method for granular enzyme preparation and application thereof |
| CN115433380A (en) * | 2022-09-03 | 2022-12-06 | 东莞市零度导热材料有限公司 | Manufacturing process of low-volatility heat conducting fin |
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Application publication date: 20100825 |