CN103864538A - Production method for moisturizing antibacterial dendrobium candidum culture medium containing nano-material - Google Patents
Production method for moisturizing antibacterial dendrobium candidum culture medium containing nano-material Download PDFInfo
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- CN103864538A CN103864538A CN201410134770.5A CN201410134770A CN103864538A CN 103864538 A CN103864538 A CN 103864538A CN 201410134770 A CN201410134770 A CN 201410134770A CN 103864538 A CN103864538 A CN 103864538A
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- Prior art keywords
- nano material
- nano
- antibacterial
- moisturizing
- culture medium
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- 239000002086 nanomaterial Substances 0.000 title claims abstract description 37
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 17
- 230000003020 moisturizing effect Effects 0.000 title claims abstract description 16
- 238000004519 manufacturing process Methods 0.000 title abstract description 5
- 239000001963 growth medium Substances 0.000 title abstract 4
- 241000026010 Dendrobium candidum Species 0.000 title abstract 3
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 10
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 10
- 239000000725 suspension Substances 0.000 claims abstract description 7
- 239000003610 charcoal Substances 0.000 claims abstract description 6
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 6
- 239000010455 vermiculite Substances 0.000 claims abstract description 6
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 5
- 238000001035 drying Methods 0.000 claims abstract description 4
- 239000011159 matrix material Substances 0.000 claims description 16
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 14
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 12
- 241001523681 Dendrobium Species 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 239000006185 dispersion Substances 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 8
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 claims description 7
- -1 polyoxyethylene Polymers 0.000 claims description 7
- 229920000604 Polyethylene Glycol 200 Polymers 0.000 claims description 6
- 229910010413 TiO 2 Inorganic materials 0.000 claims description 6
- 229910052681 coesite Inorganic materials 0.000 claims description 6
- 229910052906 cristobalite Inorganic materials 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 229920000136 polysorbate Polymers 0.000 claims description 6
- 239000000377 silicon dioxide Substances 0.000 claims description 6
- 235000012239 silicon dioxide Nutrition 0.000 claims description 6
- 229910052682 stishovite Inorganic materials 0.000 claims description 6
- 229910052905 tridymite Inorganic materials 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 229920002565 Polyethylene Glycol 400 Polymers 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- JLFNLZLINWHATN-UHFFFAOYSA-N pentaethylene glycol Chemical compound OCCOCCOCCOCCOCCO JLFNLZLINWHATN-UHFFFAOYSA-N 0.000 claims description 2
- 238000002156 mixing Methods 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 2
- 239000002202 Polyethylene glycol Substances 0.000 abstract 1
- 239000002270 dispersing agent Substances 0.000 abstract 1
- 229920001223 polyethylene glycol Polymers 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a production method for a moisturizing antibacterial dendrobium candidum culture medium containing a nano-material. The production method comprises the following steps: adding 2-2.5g of nanomaterial, 70-100g of polyethylene glycol, 70-100g of polyvinyl alcohol (PVA) and 3-5g of dispersing agent into per liter of water to produce a nano-material suspension, evenly stirring, then putting the mixture in an ultrasonic container, forcibly dispersing by virtue of 50-70W/L of ultrasonic wave with the ultrasonic frequency of 30-50KHz for two to three minutes, immersing and mixing the high-temperature sterilized barks, vermiculite and charcoal with dispersed nano-material suspension at a certain ratio of 1:(3-5), standing and adsorbing for 8-10 hours, fishing out and drying to obtain the moisturizing antibacterial dendrobium candidum culture medium containing the nano-material. The culture medium containing the nano-material has moisturizing antibacterial functions, can prevent mould, and is beneficial to transplantation of endrobium candidum seedlings, and the survival rate can achieve more than 95%.
Description
Technical field
The present invention relates to Herba Dendrobii planting technology field, relate in particular to a kind of making method of the antibacterial officinal dendrobium stem plantation matrix of moisturizing containing nano material.
Background technology
" Chinese pharmacy dictionary " claims Herba Dendrobii " gas-liquid of specially growing lung stomach, gas-liquid is rushed prosperous, kidney water is spontaneous ", says that it is good at nourishing Yin and promoting production of body fluid, all diseases for the treatment of Yin and fluid deficiency.Herba Dendrobii except enriching yin, the function nourishing blood in addition, Qing Dynasty's " property of medicine opinion " says the long-pending essence of Herba Dendrobii energy kidney tonifying, nourishing stomach-YIN, beneficial strength.Iron sheet stone solution contains abundant polysaccharose substance, can promote total balance of the body, effectively build up health, thus effective strengthening immunity.In sum, Herba Dendrobii has huge commercial value, but because its wild resource rareness and artificial growth still has many technical bottlenecks, therefore numerous experts and scholars have launched unremitting research and trial to its artificial growth.
Cultivation matrix is the key of High efficiency and high quality cultivation, the biological nature of Herba Dendrobii requires the existing good water-retentivity of cultivation matrix to have again ventilating air permeability, so must prevent from occurring in planting matrix putrid and deteriorated, generally edible as healthcare products or medicine because of Herba Dendrobii, avoid in principle spraying sterilization and disinfection agricultural chemicals as far as possible.Herba Dendrobii planting matrix adopts the mixing of sandstone, vegetable mould, leaf, bark etc. more at present, and ventilation property and antibacterial ability are not good especially.
Summary of the invention
For the deficiencies in the prior art, the invention reside in the making method that a kind of antibacterial officinal dendrobium stem plantation matrix of moisturizing containing nano material is provided.
For achieving the above object, the present invention is achieved by following technical proposals:
A making method that contains the antibacterial officinal dendrobium stem plantation matrix of moisturizing of nano material, material therefor is bark, vermiculite, charcoal and nano material.
A kind of making method of the antibacterial officinal dendrobium stem plantation matrix of moisturizing containing nano material is as follows: by nano material, polyoxyethylene glycol, polyvinyl alcohol (PVA), dispersion agent adds 2 ~ 2.5g according to every premium on currency, 70 ~ 100g, 70 ~ 100g, 3 ~ 5g above-mentioned materials preparing nano material suspension, stir evenly, put into again ultrasonic container, with ultrasonic frequency 30 ~ 50KHZ, the forced ultrasonic of 50 ~ 70W/L disperses 2 ~ 3 minutes, make the outstanding mixed liquid of nano material, by the bark after high-temperature sterilization, vermiculite, charcoal immerses and mixes according to the volume ratio of 1:3 ~ 5 with the outstanding mixed liquid of nano material of dispersion, standing adsorption 8 ~ 10 hours, after picking up, be drying to obtain.
The each constituent mass proportioning of described nano material suspension.
Described nano material adopts one or more in nano TiO 2, nanometer SiO2, nano-ZnO.
Described polyoxyethylene glycol adopts one or both in PEG200, PEG400.
Described dispersion agent adopts one or both in tween, sapn.
Beneficial effect of the present invention is: this cultivation matrix containing nano material has moisturizing antibacterial, can prevent from going mouldy, and is conducive to the transplanting of Herba Dendrobii seedling, and surviving rate can reach more than 95%.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.
A kind of concrete making step of making method of the antibacterial officinal dendrobium stem plantation matrix of moisturizing containing nano material is as follows:
First, calculate and prepare bark, vermiculite, the charcoal material of suitable quantity, and above-mentioned materials is carried out to high-temperature sterilization, for subsequent use, if conditions permit, bark shifts to an earlier date fermentation maturity half a year, for subsequent use.
Then, calculate and prepare nano material (nano TiO 2, nanometer SiO2, nano-ZnO), polyoxyethylene glycol (PEG200/400), polyvinyl alcohol (PVA), four kinds of medicines of dispersion agent (tween or sapn), by nano material (nano TiO 2, nanometer SiO2, nano-ZnO), polyoxyethylene glycol (PEG200/400), polyvinyl alcohol (PVA), dispersion agent (tween or sapn) adds 2-2.5g according to every premium on currency, 70-100g, 70-100g, 3-5g above-mentioned materials preparing nano material suspension, stir evenly, put into again ultrasonic stirring tank, use ultrasonic frequency 30-50KHZ, the forced ultrasonic of 50-70W/L disperses 2-3 minute, can make nano material suspension.In the present embodiment, nano material is used nano TiO 2, nanometer SiO2, nano-ZnO mixing material, polyoxyethylene glycol adopts PEG200, and dispersion agent uses tween, in actual production, can adopt nano TiO 2 according to factors such as material prices, nanometer SiO2, in nano-ZnO, one or more mix, one or both in PEG200, PEG 400, one or both in tween, sapn.
Finally, the bark after high-temperature sterilization, vermiculite, charcoal are put into mixing pit mixing with the nano material water slurry of dispersion together with the volume ratio of 1:3 ~ 5, standing adsorption 8-10 hour, is drying to obtain after picking up.
While containing the making method plantation Herba Dendrobii of the antibacterial officinal dendrobium stem plantation matrix of moisturizing of nano material, then first simple agitation is layered on the cement pit or other planting containers with discharge structure, then after spray water or nutritive medium, can plant Herba Dendrobii.
That in above-described embodiment and specification sheets, describes just illustrates principle of the present invention and most preferred embodiment; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.
Claims (4)
1. the making method containing the antibacterial officinal dendrobium stem plantation matrix of moisturizing of nano material is as follows: by nano material, polyoxyethylene glycol, polyvinyl alcohol (PVA), dispersion agent adds 2 ~ 2.5g according to every premium on currency, 70 ~ 100g, 70 ~ 100g, 3 ~ 5g above-mentioned materials preparing nano material suspension, stir evenly, put into again ultrasonic container, with ultrasonic frequency 30 ~ 50KHZ, the forced ultrasonic of 50 ~ 70W/L disperses 2 ~ 3 minutes, make the outstanding mixed liquid of nano material, by the bark after high-temperature sterilization, vermiculite, charcoal immerses and mixes according to the volume ratio of 1:3 ~ 5 with the outstanding mixed liquid of nano material of dispersion, standing adsorption 8 ~ 10 hours, after picking up, be drying to obtain.
2. the making method of a kind of antibacterial officinal dendrobium stem plantation matrix of moisturizing containing nano material according to claim 1, is characterized in that: described nano material adopts one or more in nano TiO 2, nanometer SiO2, nano-ZnO.
3. the making method of a kind of antibacterial officinal dendrobium stem plantation matrix of moisturizing containing nano material according to claim 1, is characterized in that: described polyoxyethylene glycol adopts one or both in PEG200, PEG400.
4. the making method of a kind of antibacterial officinal dendrobium stem plantation matrix of moisturizing containing nano material according to claim 1, is characterized in that: described dispersion agent adopts tween or sapn.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410134770.5A CN103864538B (en) | 2014-04-04 | 2014-04-04 | A kind of making method of the antibacterial dendrobium officinale culture medium of moisturizing containing nano material |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410134770.5A CN103864538B (en) | 2014-04-04 | 2014-04-04 | A kind of making method of the antibacterial dendrobium officinale culture medium of moisturizing containing nano material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103864538A true CN103864538A (en) | 2014-06-18 |
| CN103864538B CN103864538B (en) | 2016-01-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410134770.5A Expired - Fee Related CN103864538B (en) | 2014-04-04 | 2014-04-04 | A kind of making method of the antibacterial dendrobium officinale culture medium of moisturizing containing nano material |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604688A (en) * | 2015-02-05 | 2015-05-13 | 东莞广州中医药大学中医药数理工程研究院 | In-vitro culture method capable of promoting efficient propagation of dendrobium officinale by nano materials having antibacterial effect |
| CN104604659A (en) * | 2015-02-11 | 2015-05-13 | 贵州绿春缘有机农业产业管理有限公司 | Culture media for improving polysaccharide content of dendrobium officinale plants and culture method of dendrobium officinale plants |
| CN110771485A (en) * | 2019-10-22 | 2020-02-11 | 丽水学院 | Dendrobium officinale cultivation method for rooting and promoting germination by tying trees |
| CN117923978A (en) * | 2023-11-16 | 2024-04-26 | 四川中农润泽生物科技有限公司 | Organic fertilizer with function of inhibiting plant diseases and insect pests and preparation method thereof |
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| CN103693674A (en) * | 2013-11-27 | 2014-04-02 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of nanometer zinc oxide composite dispersion liquid |
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- 2014-04-04 CN CN201410134770.5A patent/CN103864538B/en not_active Expired - Fee Related
Patent Citations (9)
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| CN101132858A (en) * | 2004-11-17 | 2008-02-27 | 上游技术革新有限公司 | Multicomponent nanoparticles formed using a dispersing agent |
| CN1961652A (en) * | 2005-11-07 | 2007-05-16 | 中国医学科学院药用植物研究所 | Application of mycorrhizal fungi in large-sclae plantation of dendrobium stem |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604688A (en) * | 2015-02-05 | 2015-05-13 | 东莞广州中医药大学中医药数理工程研究院 | In-vitro culture method capable of promoting efficient propagation of dendrobium officinale by nano materials having antibacterial effect |
| CN104604659A (en) * | 2015-02-11 | 2015-05-13 | 贵州绿春缘有机农业产业管理有限公司 | Culture media for improving polysaccharide content of dendrobium officinale plants and culture method of dendrobium officinale plants |
| CN104604659B (en) * | 2015-02-11 | 2017-10-10 | 贵州绿春缘有机农业产业管理有限公司 | A kind of cultivation matrix and a kind of cultural method of dendrobium candidum plant for being used to improve dendrobium candidum plant polyoses content |
| CN110771485A (en) * | 2019-10-22 | 2020-02-11 | 丽水学院 | Dendrobium officinale cultivation method for rooting and promoting germination by tying trees |
| CN117923978A (en) * | 2023-11-16 | 2024-04-26 | 四川中农润泽生物科技有限公司 | Organic fertilizer with function of inhibiting plant diseases and insect pests and preparation method thereof |
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| Publication number | Publication date |
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| CN103864538B (en) | 2016-01-27 |
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Effective date of registration: 20220228 Address after: No.2, Libin Road, Songshanhu science and Technology Industrial Park, Dongguan, Guangdong 523000 Patentee after: Cai Pingping Address before: 523808 room 305-310, building 8, innovation and Technology Park, Songshanhu high tech Industrial Development Zone, Dongguan City, Guangdong Province Patentee before: GUANGDONG GUOFANG MEDICAL TECHNOLOGY Co.,Ltd. |
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