CN103858821B - A kind of feeding method and nourishing additive agent increasing milch goat X sperm ratio - Google Patents
A kind of feeding method and nourishing additive agent increasing milch goat X sperm ratio Download PDFInfo
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Abstract
The invention discloses a kind of feeding method and the nourishing additive agent that increase milch goat X sperm ratio, by male milch goat wean in the 6th week, then following 5 vegetative stages its process of growth are divided into feed, basic ration is fed every day at each vegetative stage, described basic ration comprises hay and mixed concentrate, and with the mixed concentrate in basic ration for benchmark in supplementary ratio carry out folic acid, VB12 and methionine supplement, supplement ratio from wean after-stage to body maturation phase stage step wise reduction.The present invention can directly utilize external nourishment factor to increase milch goat X sperm ratio, thus reach easy, realize the separation of X sperm in sperm rapidly, considerably reduce separation costs, more female embryo can be obtained, thus contribute to improving aquaculture economic benefit, simultaneously being also conducive to property control techniques in the promotion and application in livestock breeding field.
Description
Technical field
The invention belongs to animal raising technology field, relate to a kind of feeding method and the nourishing additive agent that increase milch goat X sperm ratio.
Background technology
Sexual control is a technology that can significantly improve aquaculture economic benefit.Mainly realized by XY spermatozoa isolation and Early-stage judgment at present.Be fluidic cell partition method about the most effective method of XY spermatozoa isolation, but this technology separation finite rate, separation costs is expensive, and the equipment of specialization limits its application aborning, can not meet the wilderness demand to sexing semen on producing far away; H y antigen partition method is a kind of succinct Gender preselection by comparison, but due to the specificity of h y antigen poor, separating effect can not reach the satisfied requirement of people all the time.Early-stage judgment must adopt Protocols in Molecular Biology, higher to the requirement of personnel, and destroys the structure of embryo.
Ruminant has direct relation to reproductive performance and trophic factor, for the breeding of male ruminant, will apparently higher than growing up to the sensitivity of nutrition before sexual maturity, and this nutrition will be permanent for the histological change of testis.This impact of nutrition on testis is by changing the bent thin size of quiet pipe and the generation of sperm thus affecting reproductive performance.Contain a large amount of spermatogonium in testis during buck birth, along with growing to sexual maturity further, spermatogonium, while a series of differentiation, has a large amount of spermatids be degraded and absorb, and only has part to form production of sperm.
Summary of the invention
The present invention solve problem be to provide a kind of feeding method and the nourishing additive agent that increase milch goat X sperm ratio, by milch goat rationally feed improve X sperm ratio, thus easy, obtain more female embryo rapidly.
The present invention is achieved through the following technical solutions:
Increase a feeding method for milch goat X sperm ratio, comprise following operation:
By male milch goat wean in the 6th week, then its process of growth be divided into following 5 vegetative stages to feed: 7th ~ 13 weeks for after wean, 14th ~ 19 weeks for puberty, 20th ~ 25 weeks be that to join phase, the 31st week rear be the body maturation phase for sexual maturing period, 26th ~ 30 Zhou Weichu;
Basic ration is fed every day at each vegetative stage, described basic ration comprises hay and mixed concentrate, and with the mixed concentrate in basic ration for benchmark in supplementary ratio carry out folic acid, VB12 and methionine supplement, supplement ratio from wean after-stage to body maturation phase stage step wise reduction;
By quality ratio, in wean after-stage, the supplementary ratio of folic acid is 3.0 ~ 4.0 ‰ of mixed concentrate, and ratio of supplementing is successively decreased with 0.5 ~ 1.0 ‰ to body maturation phase stage rank Zi wean after-stage;
The magnitude of recruitment of VB12 is 1/60 ~ 1/40 of folic acid, and the magnitude of recruitment of methionine is 1/2 ~ 1/3 of folic acid.
Further, Zi wean after-stage to body maturation phase stage rank, the supplementary ratio of described folic acid, VB12 and methionine is as follows:
After wean: the methionine of the folic acid of 3.0 ~ 4.0 ‰, VB12 and 1.2 ~ 2.0 ‰ of 0.07 ~ 0.09 ‰;
Puberty: the methionine of the folic acid of 2.0 ~ 3.0 ‰, VB12 and 0.8 ~ 1.5 ‰ of 0.05 ~ 0.06 ‰;
Sexual maturing period: the methionine of the folic acid of 1.5 ~ 2.5 ‰, VB12 and 0.6 ~ 1.2 ‰ of 0.04 ~ 0.05 ‰;
Just join the phase: the methionine of the folic acid of 1.0 ~ 2.0 ‰, VB12 and 0.5 ~ 0.8 ‰ of 0.03 ~ 0.04 ‰;
The body maturation phase: the methionine of the folic acid of 0.8 ~ 1.2 ‰, VB12 and 0.3 ~ 0.5 ‰ of 0.01 ~ 0.02 ‰.
Further, Zi wean after-stage to body maturation phase stage rank, the supplementary ratio of folic acid is successively decreased with 0.5 ‰, and the supplementary ratio of VB12 is successively decreased with 0.01 ~ 0.015 ‰, and the supplementary ratio of methionine is successively decreased with 0.2 ~ 0.3 ‰.
Mixed concentrate in described basic ration comprises corn, wheat bran, rapeseed dregs, soya-bean cake, fish meal, salt and premix, and supplements bone meal in wean after-stage, stage puberty;
Wean after-stage basic ration comprises the hay of 500g, the mixed concentrate of 200g, and is equipped with the full milk of 300g; From wean after-stage to stage body maturation phase, the basic ration in per stage increases the hay of 500g, the mixed concentrate of 50g; Start supplementary 0.5 egg from stage puberty, and each stage afterwards increases by 0.5 egg.
In mass fraction, described mixed concentrate is constructed as follows:
After wean: the corn of 45%, the wheat bran of 25%, 15% rapeseed dregs, 5% soya-bean cake, the fish meal of 5%, the salt of 1% and 1% premix, and be equipped with the bone meal of 3%;
Puberty: the corn of 50%, the wheat bran of 22%, 12% rapeseed dregs, 5% soya-bean cake, the fish meal of 5%, the salt of 1% and 2% premix, and be equipped with the bone meal of 3%;
Sexual maturing period: the corn of 55%, the wheat bran of 20%, 10% rapeseed dregs, 7% soya-bean cake, the fish meal of 5%, the salt of 1% and 2% premix;
Just join the phase: the corn of 55%, the wheat bran of 20%, 10% rapeseed dregs, 7% soya-bean cake, the fish meal of 5%, the salt of 1% and 2% premix;
The body maturation phase: the corn of 60%, the wheat bran of 15%, 10% rapeseed dregs, 7% soya-bean cake, the fish meal of 5%, the salt of 1% and 2% premix.
Described after reaching and just joining phase or body maturation, gather the seminal fluid of male milch goat respectively, first carry out the ratio analysis of X/Y sperm, be then separated X sperm and carry out in vitro fertilization.
A kind of nourishing additive agent increasing milch goat X sperm ratio, the 1st section of nourishing additive agent be made up of folic acid, VB12 and methionine, in mass fraction, comprise the folic acid of 3.0 ~ 4.0 ‰ of 1 section of datum quantity, 0.07 ~ 0.09 ‰ the methionine of VB12 and 1.2 ~ 2.0 ‰, 1 section of datum quantity is 180 ~ 200g.
Also comprise the 2nd section of nourishing additive agent; In mass fraction, the 2nd section of described nourishing additive agent comprises the methionine of the folic acid of 2.0 ~ 3.0 ‰ of 2 sections of datum quantities, VB12 and 0.8 ~ 1.5 ‰ of 0.05 ~ 0.06 ‰; 2 sections of datum quantities are 220 ~ 250g;
Also comprise the 3rd section of nourishing additive agent; In mass fraction, the 3rd section of described nourishing additive agent comprises the methionine of the folic acid of 1.5 ~ 2.5 ‰ of 3 sections of datum quantities, VB12 and 0.6 ~ 1.2 ‰ of 0.04 ~ 0.05 ‰; 3 sections of datum quantities are 300 ~ 350g.
Also comprise the 4th section of nourishing additive agent, the 5th section of nourishing additive agent; In mass fraction:
The 4th section of described nourishing additive agent comprises the methionine of the folic acid of 1.0 ~ 2.0 ‰ of 4 sections of datum quantities, VB12 and 0.5 ~ 0.8 ‰ of 0.03 ~ 0.04 ‰; 4 sections of datum quantities are 450 ~ 500g;
The 5th section of described foster agent comprises the methionine of the folic acid of 0.8 ~ 1.2 part of 5 sections of datum quantities, 0.01 ~ 0.02 part VB12 and 0.3 ~ 0.5 part; 5 sections of datum quantities are 500 ~ 550g.
Compared with prior art, the present invention has following useful technique effect:
The feeding method of increase milch goat X sperm ratio provided by the invention and nourishing additive agent, external nourishment factor can be directly utilized to increase milch goat X sperm ratio, thus reach easy, realize the separation of X sperm in sperm rapidly, considerably reduce separation costs, more female embryo can be obtained, thus contribute to improving aquaculture economic benefit, simultaneously being also conducive to property control techniques in the promotion and application in livestock breeding field.
The feeding method of increase milch goat X sperm ratio provided by the invention, after the male milch goat process of growth after wean is divided into wean, puberty, sexual maturity, just joins phase and 5 stages of body maturation.Each stage adds folic acid, methionine and VB12 as nourishing additive agent on the basis of basic ration.Just joining and body maturation stage artificial collecting semen respectively, find just joining stage sperm viability more than 82%, X sperm content is 77.5 ~ 80.0%, and fertilization rate is more than 84.4%, rear female embryo ratio in vitro fertilization is 93.4%, and growth is 56.9% to the ratio of blastaea; After body maturation, sperm viability is more than 84%, and X sperm content is 73.1 ~ 76.1%, and fertilization rate is more than 88.2%, and rear female embryo ratio in vitro fertilization is 93.2%, and growth arrives the ratio of blastaea more than 55.4%.The method male milch goat of feeding can significantly improve X sperm ratio, thus obtains more female embryo.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Mammal spermatogonium is initial in reduction division, and for preventing some enzyme and sex chromosome from having an effect, two sex chromosome high enrichment form gonosome, and protectiveness chromosome is not destroyed, and closes the expression of sex chromosome specific gene.
And transcriptome analysis is carried out to milch goat testicle spermatogonia, first spermatocyte, secondary spermatocyte, result shows that the Y chromosome specific gene of all expressing in the first two stage has Sry, the secondary spermatocyte stage express have Ubely; What X chromosome specific gene was all expressed at spermatogonium and first spermatocyte only has Xist, and can also Ube1x and MHR6A be detected in the secondary spermatocyte stage.
Due to the sperm destiny containing X or Y chromosome be decided by initial meiosis before the formation of i.e. first spermatocyte.In addition, the expression of Sry gene has individual peak value when reduction division, is to determine that spermatocyte normal differentiation becomes the prerequisite of Y type spermoblast, and it must play a role at the time point that initial meiosis one is crucial.And be methylate to be modified to master about the research between nutrition, epigenetic modification and function, can according to epigenetics principle, before and after secondary spermatocyte is formed, the activity of Sry gene is intervened, thus promoting containing the spermatocytal degraded of Y chromosome and absorption, the ratio increased containing X chromosome sperm is feasible.
In order to the activity from nutrition aspect to Sry gene is intervened, folic acid, methionine and VB12 is adopted to carry out nutrition interpolation:
Folic acid, cobalamin (VB12) and methionine etc. participate in one carbon metabolism, increase the amount of adenosylmethionine, for methylated carrying out provides original methyl donor (-methyl).Adenosylmethionine is the main epigenetic mode of one of regulate gene expression to DNA methylation, and DNA shows as the cytosine methylation on CpG island.Methionine and folic acid are a main carbon donor in methylation procedure, and cobalamin is main co-factor.
Therefore following feeding method and nourishing additive agent is provided.
Increase a feeding method for milch goat X sperm ratio, comprise following operation:
By male milch goat wean in the 6th week, then its process of growth be divided into following 5 vegetative stages to feed: 7th ~ 13 weeks for after wean, 14th ~ 19 weeks for puberty, 20th ~ 25 weeks be that to join phase, the 31st week rear be the body maturation phase for sexual maturing period, 26th ~ 30 Zhou Weichu;
Basic ration is fed every day at each vegetative stage, described basic ration comprises hay and mixed concentrate, and with the mixed concentrate in basic ration for benchmark in supplementary ratio carry out folic acid, VB12 and methionine supplement, supplement ratio from wean after-stage to body maturation phase stage step wise reduction;
By quality ratio, in wean after-stage, the supplementary ratio of folic acid is 3.0 ~ 4.0 ‰ of mixed concentrate, and ratio of supplementing is successively decreased with 0.5 ~ 1.0 ‰ to body maturation phase stage rank Zi wean after-stage;
The magnitude of recruitment of VB12 is 1/60 ~ 1/40 of folic acid, and the magnitude of recruitment of methionine is 1/2 ~ 1/3 of folic acid.
Further the supplementary ratio of folic acid, VB12 and methionine is described as follows:
After wean: the methionine of the folic acid of 3.0 ~ 4.0 ‰, VB12 and 1.2 ~ 2.0 ‰ of 0.07 ~ 0.09 ‰;
Puberty: the methionine of the folic acid of 2.0 ~ 3.0 ‰, VB12 and 0.8 ~ 1.5 ‰ of 0.05 ~ 0.06 ‰;
Sexual maturing period: the methionine of the folic acid of 1.5 ~ 2.5 ‰, VB12 and 0.6 ~ 1.2 ‰ of 0.04 ~ 0.05 ‰;
Just join the phase: the methionine of the folic acid of 1.0 ~ 2.0 ‰, VB12 and 0.5 ~ 0.8 ‰ of 0.03 ~ 0.04 ‰;
The body maturation phase: the methionine of the folic acid of 0.8 ~ 1.2 ‰, VB12 and 0.3 ~ 0.5 ‰ of 0.01 ~ 0.02 ‰.
Concrete, Zi wean after-stage to body maturation phase stage rank, the supplementary ratio of folic acid is successively decreased with 0.5 ‰, and the supplementary ratio of VB12 is successively decreased with 0.01 ~ 0.015 ‰, and the supplementary ratio of methionine is successively decreased with 0.2 ~ 0.3 ‰.
It is pointed out that above-mentioned basic ration can be fed with reference to the feeding method of existing milch goat, as long as carry out nutritional supplementation according to the ratio of given nourishing additive agent and intervene.
Provide concrete basic ration, nourishing additive agent and zoopery below.Wherein basic ration, nourishing additive agent are non-limiting to further instruction of the present invention.
1, laboratory animal
Male milch goat 30, Birth weight is 2.5 ± 0.3kg.Be equally divided into two groups, control group and experimental group.
2, material
Folic acid (food-grade, content more than 99%), cobalamin and VB12 (food-grade, content more than 99%), methionine (feed grade DL-METHIONINE, content more than 98.5%).
3, feeding method
1) milch goat wean in the 6th week, is then divided into wean rear (7th ~ 13 weeks), puberty (14th ~ 19 weeks) sexual maturity (20th ~ 25 weeks), just joins the phase 5 stages of (26th ~ 30 weeks) and body maturation (after the 31st week) by its process of growth.Each stage basic ration formula is in table 1.
Table 1 each stage basic ration formula table
Described premix is:
The premix fine fodder of weaning period 1%: sulfur acid zinc 0.5mg, manganese sulphate 0.15mg, magnesia 0.4mg, Potassiumiodate 0.01mg, dehydroretinol 00 international unit, calciferol 00 international unit, vitamin e1 0 international unit;
Puberty, sexual maturity, just join the premix fine fodder of phase and body maturation phase 2%: containing cupric sulfate pentahydrate 2.5mg, ferrous sulfate heptahydrate 6mg, white vitriol 12mg, manganese sulphate 10mg, potassium iodide 0.01mg, sodium selenite 0.02mg, cobalt chloride 0.03mg.
2) at each vegetative stage Supplement of folic acid, VB12 and methionine, magnitude of recruitment with the amount of mixed concentrate in basic ration for radix, as shown in table 2.
The addition of each vegetative stage folic acid of table 2., VB12 and methionine
3) collection of seminal fluid
Just join the phase, continuous semen collection 3 times, every other day once; Continuous semen collection 3 times after body maturation, once a day.Join phase or body maturation at the beginning of reaching after, gather the seminal fluid of male milch goat respectively, first carry out the ratio analysis of X/Y sperm, be then separated X sperm and carry out in vitro fertilization.To sperm viability, X and y sperm content and fertility testing result as shown in table 3.
Table 3. manually gathers fresh essence, the parameters such as sperm viability, Y/X sperm content and fertilization rate
Note: N only numbers for testing sheep, and C is control group sheep numbering; Fertilization rate refer in vitro fertilization after grow in incubator and account for total embryo's percentage to the embryo of 2-cell stage; X/Y sperm adopts flow cytometer to be separated; After fertilization embryo adopts nest-type PRC to carry out sex-screening.
Result show the male milch goat process of growth after by wean be divided into wean after, puberty, sexual maturity, just join phase and 5 stages of body maturation.Each stage adds folic acid, methionine and VB12 on the basis of basic ration.Just joining and body maturation stage artificial collecting semen respectively, find just joining stage sperm viability more than 82%, X sperm content is 77.5 ~ 80.0%, and fertilization rate is more than 84.4%, rear female embryo ratio in vitro fertilization is 93.4%, and growth is 56.9% to the ratio of blastaea; After body maturation, sperm viability is more than 84%, and X sperm content is 73.1 ~ 76.1%, and fertilization rate is more than 88.2%, and rear female embryo ratio in vitro fertilization is 93.2%, and growth arrives the ratio of blastaea more than 55.4%.The method male milch goat of feeding can significantly improve X sperm ratio, thus obtains more female embryo.
According to above-mentioned nourishing additive agent interpolation and feed, propose the following nourishing additive agent increasing milch goat X sperm ratio, comprise 1st ~ 5 sections of nourishing additive agents, vegetative stages corresponding different respectively, in mass fraction, is specially:
1st section of nourishing additive agent, comprise the folic acid of 3.0 ~ 4.0 ‰ of 1 section of datum quantity, 0.07 ~ 0.09 ‰ the methionine of VB12 and 1.2 ~ 2.0 ‰, 1 section of datum quantity is 180 ~ 200g.
2nd section of nourishing additive agent, comprise the folic acid of 2.0 ~ 3.0 ‰ of 2 sections of datum quantities, 0.05 ~ 0.06 ‰ the methionine of VB12 and 0.8 ~ 1.5 ‰; 2 sections of datum quantities are 220 ~ 250g;
Comprise the 3rd section of nourishing additive agent, comprise the folic acid of 1.5 ~ 2.5 ‰ of 3 sections of datum quantities, 0.04 ~ 0.05 ‰ the methionine of VB12 and 0.6 ~ 1.2 ‰; 3 sections of datum quantities are 300 ~ 350g.
4th section of nourishing additive agent, comprise the folic acid of 1.0 ~ 2.0 ‰ of 4 sections of datum quantities, 0.03 ~ 0.04 ‰ the methionine of VB12 and 0.5 ~ 0.8 ‰; 4 sections of datum quantities are 450 ~ 500g;
5th section of foster agent, comprises the folic acid of 0.8 ~ 1.2 part of 5 sections of datum quantities, the methionine of VB12 and 0.3 ~ 0.5 part of 0.01 ~ 0.02 part; 5 sections of datum quantities are 500 ~ 550g.
Claims (9)
1. increase a feeding method for milch goat X sperm ratio, it is characterized in that, comprise following operation:
By male milch goat wean in the 6th week, then its process of growth be divided into following 5 vegetative stages to feed: 7th ~ 13 weeks for after wean, 14th ~ 19 weeks for puberty, 20th ~ 25 weeks be that to join phase, the 31st week rear be the body maturation phase for sexual maturing period, 26th ~ 30 Zhou Weichu;
Basic ration is fed every day at each vegetative stage, described basic ration comprises hay and mixed concentrate, and with the mixed concentrate in basic ration for benchmark in supplementary ratio carry out folic acid, VB12 and methionine supplement, supplement ratio from wean after-stage to body maturation phase stage step wise reduction;
By quality ratio, in wean after-stage, the supplementary ratio of folic acid is 3.0 ~ 4.0 ‰ of mixed concentrate, and ratio of supplementing is successively decreased with 0.5 ~ 1.0 ‰ to body maturation phase stage rank Zi wean after-stage;
The magnitude of recruitment of VB12 is 1/60 ~ 1/40 of folic acid, and the magnitude of recruitment of methionine is 1/2 ~ 1/3 of folic acid.
2. the feeding method increasing milch goat X sperm ratio as claimed in claim 1, is characterized in that, Zi wean after-stage to body maturation phase stage rank, the supplementary ratio of described folic acid, VB12 and methionine is as follows:
After wean: the methionine of the folic acid of 3.0 ~ 4.0 ‰, VB12 and 1.2 ~ 2.0 ‰ of 0.07 ~ 0.09 ‰;
Puberty: the methionine of the folic acid of 2.0 ~ 3.0 ‰, VB12 and 0.8 ~ 1.5 ‰ of 0.05 ~ 0.06 ‰;
Sexual maturing period: the methionine of the folic acid of 1.5 ~ 2.5 ‰, VB12 and 0.6 ~ 1.2 ‰ of 0.04 ~ 0.05 ‰;
Just join the phase: the methionine of the folic acid of 1.0 ~ 2.0 ‰, VB12 and 0.5 ~ 0.8 ‰ of 0.03 ~ 0.04 ‰;
The body maturation phase: the methionine of the folic acid of 0.8 ~ 1.2 ‰, VB12 and 0.3 ~ 0.5 ‰ of 0.01 ~ 0.02 ‰.
3. the feeding method increasing milch goat X sperm ratio as claimed in claim 1 or 2, it is characterized in that, from wean after-stage to body maturation phase stage rank, the supplementary ratio of folic acid is successively decreased with 0.5 ‰, the supplementary ratio of VB12 is successively decreased with 0.01 ~ 0.015 ‰, and the supplementary ratio of methionine is successively decreased with 0.2 ~ 0.3 ‰.
4. the feeding method increasing milch goat X sperm ratio as claimed in claim 1 or 2, it is characterized in that, mixed concentrate in basic ration comprises corn, wheat bran, rapeseed dregs, soya-bean cake, fish meal, salt and premix, and supplements bone meal in wean after-stage, stage puberty;
Wean after-stage basic ration comprises the hay of 500g, the mixed concentrate of 200g, and is equipped with the full milk of 300g; From wean after-stage to stage body maturation phase, the basic ration in per stage increases the hay of 500g, the mixed concentrate of 50g; Start supplementary 0.5 egg from stage puberty, and each stage afterwards increases by 0.5 egg.
5. the feeding method increasing milch goat X sperm ratio as claimed in claim 1, it is characterized in that, in mass fraction, described mixed concentrate is constructed as follows:
After wean: the corn of 45%, the wheat bran of 25%, 15% rapeseed dregs, 5% soya-bean cake, the fish meal of 5%, the salt of 1% and 1% premix, and be equipped with the bone meal of 3%;
Puberty: the corn of 50%, the wheat bran of 22%, 12% rapeseed dregs, 5% soya-bean cake, the fish meal of 5%, the salt of 1% and 2% premix, and be equipped with the bone meal of 3%;
Sexual maturing period: the corn of 55%, the wheat bran of 20%, 10% rapeseed dregs, 7% soya-bean cake, the fish meal of 5%, the salt of 1% and 2% premix;
Just join the phase: the corn of 55%, the wheat bran of 20%, 10% rapeseed dregs, 7% soya-bean cake, the fish meal of 5%, the salt of 1% and 2% premix;
The body maturation phase: the corn of 60%, the wheat bran of 15%, 10% rapeseed dregs, 7% soya-bean cake, the fish meal of 5%, the salt of 1% and 2% premix.
6. the feeding method increasing milch goat X sperm ratio as claimed in claim 1, is characterized in that, after joining phase or body maturation, gather the seminal fluid of male milch goat respectively, first carry out the ratio analysis of X/Y sperm at the beginning of reaching, be then separated X sperm and carry out in vitro fertilization.
7. one kind increases the nourishing additive agent of milch goat X sperm ratio, it is characterized in that, the 1st section of nourishing additive agent be made up of folic acid, VB12 and methionine, in mass fraction, comprise the folic acid of 3.0 ~ 4.0 ‰ of 1 section of datum quantity, 0.07 ~ 0.09 ‰ the methionine of VB12 and 1.2 ~ 2.0 ‰, 1 section of datum quantity is 180 ~ 200g; Described 1st section refers to latter 7th ~ 13 weeks of milch goat birth;
Also comprise the 2nd section of nourishing additive agent; In mass fraction, the 2nd section of described nourishing additive agent comprises the methionine of the folic acid of 2.0 ~ 3.0 ‰ of 2 sections of datum quantities, VB12 and 0.8 ~ 1.5 ‰ of 0.05 ~ 0.06 ‰; 2 sections of datum quantities are 220 ~ 250g, and described 2nd section refers to latter 14th ~ 19 weeks of milch goat birth.
8. the nourishing additive agent increasing milch goat X sperm ratio as claimed in claim 7, is characterized in that, also comprise the 3rd section of nourishing additive agent; In mass fraction, the 3rd section of described nourishing additive agent comprises the methionine of the folic acid of 1.5 ~ 2.5 ‰ of 3 sections of datum quantities, VB12 and 0.6 ~ 1.2 ‰ of 0.04 ~ 0.05 ‰; 3 sections of datum quantities are 300 ~ 350g, and described 3rd section refers to latter 20th ~ 25 weeks of milch goat birth.
9. the nourishing additive agent increasing milch goat X sperm ratio as claimed in claim 8, is characterized in that, also comprise the 4th section of nourishing additive agent, the 5th section of nourishing additive agent; In mass fraction:
The 4th section of described nourishing additive agent comprises the methionine of the folic acid of 1.0 ~ 2.0 ‰ of 4 sections of datum quantities, VB12 and 0.5 ~ 0.8 ‰ of 0.03 ~ 0.04 ‰; Described 4th section refers to latter 26th ~ 30 weeks of milch goat birth; 4 sections of datum quantities are 450 ~ 500g;
The 5th section of described foster agent comprises the methionine of the folic acid of 0.8 ~ 1.2 part of 5 sections of datum quantities, 0.01 ~ 0.02 part VB12 and 0.3 ~ 0.5 part; Described 5th section refers to that milch goat birth is rear after 31st week; 5 sections of datum quantities are 500 ~ 550g.
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