CN103858821A - Feeding method and nutrition additives for increasing ratio of sperms X of milk goat - Google Patents

Feeding method and nutrition additives for increasing ratio of sperms X of milk goat Download PDF

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CN103858821A
CN103858821A CN201410113923.8A CN201410113923A CN103858821A CN 103858821 A CN103858821 A CN 103858821A CN 201410113923 A CN201410113923 A CN 201410113923A CN 103858821 A CN103858821 A CN 103858821A
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stage
ratio
folic acid
methionine
sperm
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CN103858821B (en
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华松
周占琴
付明哲
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Northwest A&F University
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Abstract

The invention discloses a feeding method and nutrition additives for increasing the ratio of sperms X of a milk goat. The male milk goat is ablactated in the sixth week, then, the growth process of the male milk goat is divided into the following five growth stages for feeding, the goat is fed with basic rations in the growth stages every day, the basic rations comprise cured hay and mixed beneficiated burden materials, the mixed beneficiated burden materials in the basic rations are taken as the criterion, folic acid, VB12 and methionine are supplemented according to the supplementing proportion, and the supplementing proportion is decreased progressively stepwise from the stage after ablactation to the body maturation stage. According to the method and the nutrition additives, exterior nutritional factors can be directly utilized for increasing the ratio of the sperms X of the milk goat, therefore, the aims of simply, conveniently and rapidly separating the sperms X in sperms is achieved, the separation cost is greatly reduced, more female embryos can be obtained, therefore, the economic benefits of the breeding industry are improved, and meanwhile the sex control technology can be conveniently popularized and applied in the animal husbandry field.

Description

A kind of feeding method and nourishing additive agent that increases milch goat X sperm ratio
Technical field
The invention belongs to animal raising technology field, relate to a kind of feeding method and nourishing additive agent that increases milch goat X sperm ratio.
Background technology
Sex control is a technology that can significantly improve aquaculture economic benefit.Mainly to identify to realize by the separation of XY sperm and early embryo sex at present.Separating effective method about XY sperm is fluidic cell partition method, but this technology separating rate is limited, separation costs costliness, the device-restrictive of specialization its application aborning, can not meet far away and produce the upper wilderness demand to sexing semen; H y antigen partition method is a kind of succinct Gender preselection by comparison, but because the specificity of h y antigen is poor, separating effect can not reach the requirement that people are satisfied all the time.Early embryo sex qualification must adopt Protocols in Molecular Biology, to having relatively high expectations of personnel, and destroys embryo's structure.
Ruminant has direct relation to reproductive performance and trophic factor, for the breeding of male ruminant, will be apparently higher than growing up to the sensitivity of nutrition before sexual maturity, and it will be permanent that this nutrition changes for the histology of testis.Thereby nutrition is to affect reproductive performance by changing the bent size of thin quiet pipe and the generation of sperm on this impact of testis.When buck birth, testis contains a large amount of spermatogoniums, along with further growth is to sexual maturity, when a series of differentiation of spermatogonium process, has a large amount of spermatids be degraded and absorb, and only has part to form production of sperm.
Summary of the invention
The problem that the present invention solves is to provide a kind of feeding method and nourishing additive agent that increases milch goat X sperm ratio, by milch goat rationally feed improve X sperm ratio, thereby easy, obtain more female embryo rapidly.
The present invention is achieved through the following technical solutions:
A feeding method that increases milch goat X sperm ratio, comprises following operation:
Male milch goat, wean in the 6th week, is then divided into its process of growth to following 5 vegetative stages and feeds: within 7th~13 weeks, be after wean, within 14th~19 weeks, be that puberty, 20th~25 weeks are the body maturation phase for sexual maturing period, 26th~30 Zhou Weichu join phase, the 31st week rear;
Feed basic ration every day at each vegetative stage, described basic ration comprises hay and mixed concentrate, and carry out supplementing of folic acid, VB12 and methionine taking the mixed concentrate in basic ration as benchmark in the ratio of supplementing, the ratio of supplementing is successively decreased to body maturation phase stage staged Zi wean after-stage;
By quality ratio, in wean after-stage, the supplementary ratio of folic acid is mixed concentrate 3.0~4.0 ‰, and the ratio of supplementing is successively decreased with 0.5~1.0 ‰ to body maturation phase stage rank from the after-stage that weans;
The magnitude of recruitment of VB12 is 1/60~1/40 of folic acid, and the magnitude of recruitment of methionine is folic acid 1/2~1/3.
Further, certainly the after-stage that weans, to body maturation phase stage rank, the supplementary ratio of described folic acid, VB12 and methionine is as follows:
After wean: the methionine of VB12 and 1.2~2.0 ‰ of 3.0~4.0 ‰ folic acid, 0.07~0.09 ‰;
Puberty: the methionine of VB12 and 0.8~1.5 ‰ of 2.0~3.0 ‰ folic acid, 0.05~0.06 ‰;
Sexual maturing period: the methionine of VB12 and 0.6~1.2 ‰ of 1.5~2.5 ‰ folic acid, 0.04~0.05 ‰;
Just join the phase: the methionine of VB12 and 0.5~0.8 ‰ of 1.0~2.0 ‰ folic acid, 0.03~0.04 ‰;
The body maturation phase: the methionine of VB12 and 0.3~0.5 ‰ of 0.8~1.2 ‰ folic acid, 0.01~0.02 ‰.
Further, certainly the after-stage that weans, to body maturation phase stage rank, the supplementary ratio of folic acid is successively decreased with 0.5 ‰, and the supplementary ratio of VB12 is successively decreased with 0.01~0.015 ‰, and the supplementary ratio of methionine is successively decreased with 0.2~0.3 ‰.
Mixed concentrate in described basic ration comprises corn, wheat bran, rapeseed dregs, soya-bean cake, fish meal, salt and premix, and supplements bone meal in wean after-stage, stage puberty;
Wean after-stage basic ration comprises the hay of 500g, the mixed concentrate of 200g, and is equipped with the full milk of 300g; From wean after-stage, to stage body maturation phase, the basic ration in per stage increases the hay of 500g, the mixed concentrate of 50g; Start to supplement 0.5 egg from stage puberty, and each stage afterwards increases by 0.5 egg.
In mass fraction, described mixed concentrate is constructed as follows:
After wean: 45% corn, 25% wheat bran, 15% rapeseed dregs, 5% soya-bean cake, 5% fish meal, 1% salt and 1% premix, and be equipped with 3% bone meal;
Puberty: 50% corn, 22% wheat bran, 12% rapeseed dregs, 5% soya-bean cake, 5% fish meal, 1% salt and 2% premix, and be equipped with 3% bone meal;
Sexual maturing period: 55% corn, 20% wheat bran, 10% rapeseed dregs, 7% soya-bean cake, 5% fish meal, 1% salt and 2% premix;
Just join the phase: 55% corn, 20% wheat bran, 10% rapeseed dregs, 7% soya-bean cake, 5% fish meal, 1% salt and 2% premix;
The body maturation phase: 60% corn, 15% wheat bran, 10% rapeseed dregs, 7% soya-bean cake, 5% fish meal, 1% salt and 2% premix.
Describedly just join after phase or body maturation reaching, gather respectively the seminal fluid of male milch goat, first carry out the ratio analysis of X/Y sperm, then separate X sperm and carry out in vitro fertilization.
A kind of nourishing additive agent that increases milch goat X sperm ratio, the 1st section of nourishing additive agent being formed by folic acid, VB12 and methionine, in mass fraction, comprise the methionine of VB12 and 1.2~2.0 ‰ of folic acid, 0.07~0.09 ‰ of 3.0 of 1 section of datum quantity~4.0 ‰, 1 section of datum quantity is 180~200g.
Also comprise the 2nd section of nourishing additive agent; In mass fraction, the 2nd section of described nourishing additive agent comprises the methionine of VB12 and 0.8~1.5 ‰ of folic acid, 0.05~0.06 ‰ of 2.0 of 2 sections of datum quantities~3.0 ‰; 2 sections of datum quantities are 220~250g;
Also comprise the 3rd section of nourishing additive agent; In mass fraction, the 3rd section of described nourishing additive agent comprises the methionine of VB12 and 0.6~1.2 ‰ of folic acid, 0.04~0.05 ‰ of 1.5 of 3 sections of datum quantities~2.5 ‰; 3 sections of datum quantities are 300~350g.
Also comprise the 4th section of nourishing additive agent, the 5th section of nourishing additive agent; In mass fraction:
The 4th section of described nourishing additive agent comprises the methionine of VB12 and 0.5~0.8 ‰ of folic acid, 0.03~0.04 ‰ of 1.0 of 4 sections of datum quantities~2.0 ‰; 4 sections of datum quantities are 450~500g;
The 5th section of described foster agent comprises the folic acid of 0.8~1.2 part of 5 sections of datum quantities, the VB12 of 0.01~0.02 part and the methionine of 0.3~0.5 part; 5 sections of datum quantities are 500~550g.
Compared with prior art, the present invention has following useful technique effect:
Feeding method and the nourishing additive agent of increase milch goat X sperm ratio provided by the invention, can directly utilize extraneous trophic factor to increase milch goat X sperm ratio, thereby the separation that reach easy, realizes rapidly X sperm in sperm, significantly reduce separation costs, can obtain more female embryo, thereby contribute to improve aquaculture economic benefit, simultaneously being also conducive to property control techniques in the promotion and application in livestock breeding field.
The feeding method of increase provided by the invention milch goat X sperm ratio, is divided into the male milch goat process of growth after wean after wean, puberty, sexual maturity, just joins 5 stages of phase and body maturation.Each stage is added folic acid, methionine and VB12 as nourishing additive agent on the basis of basic ration.Just joining and body maturation stage artificial collecting semen respectively, find just joining stage sperm viability more than 82%, X sperm content is 77.5~80.0%, and fertilization rate is more than 84.4%, rear female embryo ratio in vitro fertilization is 93.4%, and growing is 56.9% to the ratio of blastaea; After body maturation, sperm viability is more than 84%, and X sperm content is 73.1~76.1%, and fertilization rate is more than 88.2%, and rear female embryo ratio in vitro fertilization is 93.2%, grows the ratio that arrives blastaea more than 55.4%.The method male milch goat of feeding can significantly improve X sperm ratio, thereby obtains more female embryo.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Mammal spermatogonium is initial in reduction division, for preventing that some enzyme and sex chromosome from having an effect, and two concentrated gonosomes that form of sex chromosome height, protectiveness chromosome is not destroyed, and has closed the expression of sex chromosome specific gene.
And milch goat testicle spermatogonia, first spermatocyte, secondary spermatocyte are transcribed to group analysis, result shows that the Y chromosome specific gene of all expressing in the first two stage has Sry, at the Ubely that has of secondary spermatocyte stage expression; What X chromosome specific gene was all expressed at spermatogonium and first spermatocyte only has Xist, and Ube1x and MHR6A can also be detected in the secondary spermatocyte stage.
Because the sperm destiny that contains X or Y chromosome is decided by that before initial meiosis be the formation of first spermatocyte.In addition, the expression of Sry gene has individual peak value in the time of reduction division, is to determine that spermatocyte normal differentiation becomes the prerequisite of Y type spermoblast, and it must play a role at crucial time point of initial meiosis.And be to methylate to be modified to master about the research between nutrition, epigenetic modification and function, can be according to epigenetics principle, before and after secondary spermatocyte forms, the activity of Sry gene is intervened, thereby promote that, containing the spermatocytal degraded of Y chromosome and absorption, it is feasible increasing containing the ratio of X chromosome sperm.
In order from nutrition aspect, the activity of Sry gene to be intervened, adopt folic acid, methionine and VB12 to carry out nutrition interpolation:
Folic acid, cobalamin (VB12) and methionine etc. participate in one carbon metabolism, increase the amount of adenosylmethionine, are the methylated original methyl donor (methyl) that provides.Adenosylmethionine is the main epigenetic mode of one of regulate gene expression to DNA methylation, shows as the cytosine methylation on CpG island on DNA.Methionine and folic acid are a main carbon donor in the process of methylating, and cobalamin is main co-factor.
Therefore provide following feeding method and nourishing additive agent.
A feeding method that increases milch goat X sperm ratio, comprises following operation:
Male milch goat, wean in the 6th week, is then divided into its process of growth to following 5 vegetative stages and feeds: within 7th~13 weeks, be after wean, within 14th~19 weeks, be that puberty, 20th~25 weeks are the body maturation phase for sexual maturing period, 26th~30 Zhou Weichu join phase, the 31st week rear;
Feed basic ration every day at each vegetative stage, described basic ration comprises hay and mixed concentrate, and carry out supplementing of folic acid, VB12 and methionine taking the mixed concentrate in basic ration as benchmark in the ratio of supplementing, the ratio of supplementing is successively decreased to body maturation phase stage staged Zi wean after-stage;
By quality ratio, in wean after-stage, the supplementary ratio of folic acid is mixed concentrate 3.0~4.0 ‰, and the ratio of supplementing is successively decreased with 0.5~1.0 ‰ to body maturation phase stage rank from the after-stage that weans;
The magnitude of recruitment of VB12 is 1/60~1/40 of folic acid, and the magnitude of recruitment of methionine is folic acid 1/2~1/3.
Further the supplementary ratio of folic acid, VB12 and methionine is described as follows:
After wean: the methionine of VB12 and 1.2~2.0 ‰ of 3.0~4.0 ‰ folic acid, 0.07~0.09 ‰;
Puberty: the methionine of VB12 and 0.8~1.5 ‰ of 2.0~3.0 ‰ folic acid, 0.05~0.06 ‰;
Sexual maturing period: the methionine of VB12 and 0.6~1.2 ‰ of 1.5~2.5 ‰ folic acid, 0.04~0.05 ‰;
Just join the phase: the methionine of VB12 and 0.5~0.8 ‰ of 1.0~2.0 ‰ folic acid, 0.03~0.04 ‰;
The body maturation phase: the methionine of VB12 and 0.3~0.5 ‰ of 0.8~1.2 ‰ folic acid, 0.01~0.02 ‰.
Concrete, certainly the after-stage that weans, to body maturation phase stage rank, the supplementary ratio of folic acid is successively decreased with 0.5 ‰, and the supplementary ratio of VB12 is successively decreased with 0.01~0.015 ‰, and the supplementary ratio of methionine is successively decreased with 0.2~0.3 ‰.
It is pointed out that above-mentioned basic ration can feed with reference to the feeding method of existing milch goat, as long as carry out nutritional supplementation and intervene according to the ratio of given nourishing additive agent.
Provide concrete basic ration, nourishing additive agent and zoopery below.Wherein basic ration, nourishing additive agent are to further instruction of the present invention and non-limiting.
1, laboratory animal
30 of male milch goats, birth is heavily 2.5 ± 0.3kg.Be equally divided into two groups, control group and experimental group.
2, material
Folic acid (food stage, content is more than 99%), cobalamin are VB12 (food stage, content is more than 99%), methionine (feed grade DL-METHIONINE, content is more than 98.5%).
3, feeding method
1) milch goat wean in the 6th week, is then divided into its process of growth wean rear (7th~13 weeks), puberty (14th~19 weeks) sexual maturity (20th~25 weeks), just joins the phase 5 stages of (26th~30 weeks) and body maturation (after the 31st week).Each stage basic ration formula is in table 1.
Basic ration formula table of each stage of table 1
Figure BDA0000482011520000071
Described premix is:
The premix fine fodder of weaning period 1%: sulfur acid zinc 0.5mg, manganese sulphate 0.15mg, magnesia 0.4mg, Potassiumiodate 0.01mg, dehydroretinol 00 international unit, calciferol 00 international unit, vitamin e1 0 international unit;
Puberty, sexual maturity, just join the premix fine fodder of phase and body maturation phase 2%: containing cupric sulfate pentahydrate 2.5mg, ferrous sulfate heptahydrate 6mg, white vitriol 12mg, manganese sulphate 10mg, potassium iodide 0.01mg, sodium selenite 0.02mg, cobalt chloride 0.03mg.
2), at each vegetative stage Supplement of folic acid, VB12 and methionine, magnitude of recruitment is taking the amount of mixed concentrate in basic ration as radix, as shown in table 2.
The addition of the each vegetative stage folic acid of table 2., VB12 and methionine
Figure BDA0000482011520000081
3) collection of seminal fluid
Just join the phase, semen collection 3 times continuously, every other day once; Continuous semen collection 3 times after body maturation, once a day.At the beginning of reaching, join after phase or body maturation, gather respectively the seminal fluid of male milch goat, first carry out the ratio analysis of X/Y sperm, then separate X sperm and carry out in vitro fertilization.To sperm viability, X and y sperm content and fertility testing result are as shown in table 3.
Artificial fresh essence, the parameters such as sperm viability, Y/X sperm content and fertilization rate of gathering of table 3.
Figure BDA0000482011520000091
Note: N only numbers for experiment sheep, and C is control group sheep numbering; Fertilization rate refer in vitro fertilization after in incubator grow account for total embryo's percentage to the embryo of 2-cell stage; X/Y sperm adopts flow cytometer to separate; After fertilization embryo adopts nest-type PRC to carry out sex-screening.
Result shows the male milch goat process of growth after wean to be divided into after wean, puberty, sexual maturity, just to join 5 stages of phase and body maturation.Each stage is added folic acid, methionine and VB12 on the basis of basic ration.Just joining and body maturation stage artificial collecting semen respectively, find just joining stage sperm viability more than 82%, X sperm content is 77.5~80.0%, and fertilization rate is more than 84.4%, rear female embryo ratio in vitro fertilization is 93.4%, and growing is 56.9% to the ratio of blastaea; After body maturation, sperm viability is more than 84%, and X sperm content is 73.1~76.1%, and fertilization rate is more than 88.2%, and rear female embryo ratio in vitro fertilization is 93.2%, grows the ratio that arrives blastaea more than 55.4%.The method male milch goat of feeding can significantly improve X sperm ratio, thereby obtains more female embryo.
According to the interpolation of above-mentioned nourishing additive agent with feed, the following nourishing additive agent that increases milch goat X sperm ratio is proposed, comprise 1st~5 sections of nourishing additive agents, corresponding different vegetative stage respectively, in mass fraction, is specially:
The 1st section of nourishing additive agent, comprises the methionine of VB12 and 1.2~2.0 ‰ of folic acid, 0.07~0.09 ‰ of 3.0 of 1 section of datum quantity~4.0 ‰, and 1 section of datum quantity is 180~200g.
The 2nd section of nourishing additive agent, comprises the methionine of VB12 and 0.8~1.5 ‰ of folic acid, 0.05~0.06 ‰ of 2.0 of 2 sections of datum quantities~3.0 ‰; 2 sections of datum quantities are 220~250g;
Comprise the 3rd section of nourishing additive agent, comprise the methionine of VB12 and 0.6~1.2 ‰ of folic acid, 0.04~0.05 ‰ of 1.5 of 3 sections of datum quantities~2.5 ‰; 3 sections of datum quantities are 300~350g.
The 4th section of nourishing additive agent, comprises the methionine of VB12 and 0.5~0.8 ‰ of folic acid, 0.03~0.04 ‰ of 1.0 of 4 sections of datum quantities~2.0 ‰; 4 sections of datum quantities are 450~500g;
The 5th section of foster agent, comprises the folic acid of 0.8~1.2 part of 5 sections of datum quantities, the VB12 of 0.01~0.02 part and the methionine of 0.3~0.5 part; 5 sections of datum quantities are 500~550g.

Claims (10)

1. a feeding method that increases milch goat X sperm ratio, is characterized in that, comprises following operation:
Male milch goat, wean in the 6th week, is then divided into its process of growth to following 5 vegetative stages and feeds: within 7th~13 weeks, be after wean, within 14th~19 weeks, be that puberty, 20th~25 weeks are the body maturation phase for sexual maturing period, 26th~30 Zhou Weichu join phase, the 31st week rear;
Feed basic ration every day at each vegetative stage, described basic ration comprises hay and mixed concentrate, and carry out supplementing of folic acid, VB12 and methionine taking the mixed concentrate in basic ration as benchmark in the ratio of supplementing, the ratio of supplementing is successively decreased to body maturation phase stage staged Zi wean after-stage;
By quality ratio, in wean after-stage, the supplementary ratio of folic acid is mixed concentrate 3.0~4.0 ‰, and the ratio of supplementing is successively decreased with 0.5~1.0 ‰ to body maturation phase stage rank from the after-stage that weans;
The magnitude of recruitment of VB12 is 1/60~1/40 of folic acid, and the magnitude of recruitment of methionine is folic acid 1/2~1/3.
2. the feeding method of increase milch goat X sperm ratio as claimed in claim 1, is characterized in that, certainly the after-stage that weans, to body maturation phase stage rank, the supplementary ratio of described folic acid, VB12 and methionine is as follows:
After wean: the methionine of VB12 and 1.2~2.0 ‰ of 3.0~4.0 ‰ folic acid, 0.07~0.09 ‰;
Puberty: the methionine of VB12 and 0.8~1.5 ‰ of 2.0~3.0 ‰ folic acid, 0.05~0.06 ‰;
Sexual maturing period: the methionine of VB12 and 0.6~1.2 ‰ of 1.5~2.5 ‰ folic acid, 0.04~0.05 ‰;
Just join the phase: the methionine of VB12 and 0.5~0.8 ‰ of 1.0~2.0 ‰ folic acid, 0.03~0.04 ‰;
The body maturation phase: the methionine of VB12 and 0.3~0.5 ‰ of 0.8~1.2 ‰ folic acid, 0.01~0.02 ‰.
3. the feeding method of increase milch goat X sperm ratio as claimed in claim 1 or 2, it is characterized in that, from wean after-stage to body maturation phase stage rank, the supplementary ratio of folic acid is successively decreased with 0.5 ‰, the supplementary ratio of VB12 is successively decreased with 0.01~0.015 ‰, and the supplementary ratio of methionine is successively decreased with 0.2~0.3 ‰.
4. the feeding method of increase milch goat X sperm ratio as claimed in claim 1 or 2, it is characterized in that, mixed concentrate in basic ration comprises corn, wheat bran, rapeseed dregs, soya-bean cake, fish meal, salt and premix, and supplements bone meal in wean after-stage, stage puberty;
Wean after-stage basic ration comprises the hay of 500g, the mixed concentrate of 200g, and is equipped with the full milk of 300g; From wean after-stage, to stage body maturation phase, the basic ration in per stage increases the hay of 500g, the mixed concentrate of 50g; Start to supplement 0.5 egg from stage puberty, and each stage afterwards increases by 0.5 egg.
5. the feeding method of the increase milch goat X sperm ratio as described in claim 1 or 4, is characterized in that, in mass fraction, described mixed concentrate is constructed as follows:
After wean: 45% corn, 25% wheat bran, 15% rapeseed dregs, 5% soya-bean cake, 5% fish meal, 1% salt and 1% premix, and be equipped with 3% bone meal;
Puberty: 50% corn, 22% wheat bran, 12% rapeseed dregs, 5% soya-bean cake, 5% fish meal, 1% salt and 2% premix, and be equipped with 3% bone meal;
Sexual maturing period: 55% corn, 20% wheat bran, 10% rapeseed dregs, 7% soya-bean cake, 5% fish meal, 1% salt and 2% premix;
Just join the phase: 55% corn, 20% wheat bran, 10% rapeseed dregs, 7% soya-bean cake, 5% fish meal, 1% salt and 2% premix;
The body maturation phase: 60% corn, 15% wheat bran, 10% rapeseed dregs, 7% soya-bean cake, 5% fish meal, 1% salt and 2% premix.
6. the feeding method of increase milch goat X sperm ratio as claimed in claim 1, is characterized in that, at the beginning of reaching, joins after phase or body maturation, gathers respectively the seminal fluid of male milch goat, first carries out the ratio analysis of X/Y sperm, then separates X sperm and carries out in vitro fertilization.
7. one kind increases the nourishing additive agent of milch goat X sperm ratio, it is characterized in that, the 1st section of nourishing additive agent being formed by folic acid, VB12 and methionine, in mass fraction, comprise the methionine of VB12 and 1.2~2.0 ‰ of folic acid, 0.07~0.09 ‰ of 3.0 of 1 section of datum quantity~4.0 ‰, 1 section of datum quantity is 180~200g.
8. the nourishing additive agent of increase milch goat X sperm ratio as claimed in claim 7, is characterized in that, also comprises the 2nd section of nourishing additive agent; In mass fraction, the 2nd section of described nourishing additive agent comprises the methionine of VB12 and 0.8~1.5 ‰ of folic acid, 0.05~0.06 ‰ of 2.0 of 2 sections of datum quantities~3.0 ‰; 2 sections of datum quantities are 220~250g.
9. the nourishing additive agent of increase milch goat X sperm ratio as claimed in claim 8, is characterized in that, also comprises the 3rd section of nourishing additive agent; In mass fraction, the 3rd section of described nourishing additive agent comprises the methionine of VB12 and 0.6~1.2 ‰ of folic acid, 0.04~0.05 ‰ of 1.5 of 3 sections of datum quantities~2.5 ‰; 3 sections of datum quantities are 300~350g.
10. the nourishing additive agent of increase milch goat X sperm ratio as claimed in claim 9, is characterized in that, also comprises the 4th section of nourishing additive agent, the 5th section of nourishing additive agent; In mass fraction:
The 4th section of described nourishing additive agent comprises the methionine of VB12 and 0.5~0.8 ‰ of folic acid, 0.03~0.04 ‰ of 1.0 of 4 sections of datum quantities~2.0 ‰; 4 sections of datum quantities are 450~500g;
The 5th section of described foster agent comprises the folic acid of 0.8~1.2 part of 5 sections of datum quantities, the VB12 of 0.01~0.02 part and the methionine of 0.3~0.5 part; 5 sections of datum quantities are 500~550g.
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