CN103843825B - Prevention plant pest Natural Circulation microbial bacterial agent and preparation method thereof - Google Patents
Prevention plant pest Natural Circulation microbial bacterial agent and preparation method thereof Download PDFInfo
- Publication number
- CN103843825B CN103843825B CN201310472045.4A CN201310472045A CN103843825B CN 103843825 B CN103843825 B CN 103843825B CN 201310472045 A CN201310472045 A CN 201310472045A CN 103843825 B CN103843825 B CN 103843825B
- Authority
- CN
- China
- Prior art keywords
- chloride
- bacterial agent
- microbial bacterial
- salt
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 29
- 230000000813 microbial effect Effects 0.000 title claims abstract description 24
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 14
- 230000002265 prevention Effects 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 33
- 150000003839 salts Chemical class 0.000 claims abstract description 11
- 241000190831 Chromatium Species 0.000 claims abstract description 10
- 241000605122 Nitrosomonas Species 0.000 claims abstract description 10
- 241000190967 Rhodospirillum Species 0.000 claims abstract description 10
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 9
- 229910002651 NO3 Inorganic materials 0.000 claims abstract description 9
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000012530 fluid Substances 0.000 claims abstract description 8
- 241001655243 Allochromatium Species 0.000 claims abstract description 5
- 241001655879 Halorhodospira Species 0.000 claims abstract description 5
- 241001353956 Lamprocystis <gastropod> Species 0.000 claims abstract description 5
- 241000605159 Nitrobacter Species 0.000 claims abstract description 5
- 241000589516 Pseudomonas Species 0.000 claims abstract description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 16
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 11
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- 239000012138 yeast extract Substances 0.000 claims description 7
- 235000019270 ammonium chloride Nutrition 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 5
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 claims description 5
- 229940052299 calcium chloride dihydrate Drugs 0.000 claims description 5
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- 239000011592 zinc chloride Substances 0.000 claims description 5
- 235000005074 zinc chloride Nutrition 0.000 claims description 5
- 241000190857 Allochromatium vinosum Species 0.000 claims description 4
- 241000605154 Nitrobacter winogradskyi Species 0.000 claims description 4
- 241000190984 Rhodospirillum rubrum Species 0.000 claims description 4
- 210000003205 muscle Anatomy 0.000 claims description 4
- 239000012531 culture fluid Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000011049 pearl Substances 0.000 claims description 3
- 241000881804 Chromatium okenii Species 0.000 claims description 2
- 241000191005 Halorhodospira halophila Species 0.000 claims description 2
- 241000420933 Lamprobacter modestohalophilus Species 0.000 claims description 2
- 241000520773 Lamprocystis roseopersicina Species 0.000 claims description 2
- 241000605121 Nitrosomonas europaea Species 0.000 claims description 2
- 241000605008 Spirillum Species 0.000 claims description 2
- 239000002775 capsule Substances 0.000 claims description 2
- 229910021591 Copper(I) chloride Inorganic materials 0.000 claims 2
- GFHNAMRJFCEERV-UHFFFAOYSA-L cobalt chloride hexahydrate Chemical compound O.O.O.O.O.O.[Cl-].[Cl-].[Co+2] GFHNAMRJFCEERV-UHFFFAOYSA-L 0.000 claims 2
- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 claims 2
- VLAPMBHFAWRUQP-UHFFFAOYSA-L molybdic acid Chemical compound O[Mo](O)(=O)=O VLAPMBHFAWRUQP-UHFFFAOYSA-L 0.000 claims 2
- NASFKTWZWDYFER-UHFFFAOYSA-N sodium;hydrate Chemical compound O.[Na] NASFKTWZWDYFER-UHFFFAOYSA-N 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 5
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 11
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 6
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 6
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 3
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 3
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 3
- 244000088415 Raphanus sativus Species 0.000 description 3
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 3
- 241000219315 Spinacia Species 0.000 description 3
- 235000009337 Spinacia oleracea Nutrition 0.000 description 3
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000011684 sodium molybdate Substances 0.000 description 3
- 235000015393 sodium molybdate Nutrition 0.000 description 3
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of prevention plant pest Natural Circulation microbial bacterial agent and preparation method, this microbial bacterial agent is by dodging Bacillus (Allochromatium), Lamorocystis (Lamprocystis), addicted to the red spiral Pseudomonas (Halorhodospira) of salt, Chromatium (Chromatium), Nitrosomonas (Nitrosomonas), nitrate bacteria belongs to (Nitrobacter), the zymotic fluid composition of Rhodospirillum (Rhodospirillum) 7 kinds of bacteriums, living bacteria count Fen others Dou≤1.0 × 10 of above 7 kinds of bacteriums in zymotic fluid
8cFU/ml.Microbial bacterial agent of the present invention has good effect to each Plants prevention disease and growth promoting effects.
Description
Technical field
The invention belongs to environmental technology field, relate to a kind of microbial bacterial agent, be specifically related to a kind of prevention plant pest Natural Circulation microbial bacterial agent and preparation method.
Background technology
Closely for decades, develop rapidly bring severe environmental pollution and ecological disruption with science and technology, social economy.The great potential of microbial degradation pollutant plays a significant role in Control pollution, repairing environment.Sewage (waste water), waste gas and solid waste that human being's production and life activity produce can process with biological method.Now in the art, also lack a kind of for preventing the microbial bacterial agent of plant pest.
Summary of the invention
Object of the present invention is exactly for the deficiencies in the prior art, provides a kind of prevention plant pest Natural Circulation microbial bacterial agent.
The present invention provides a kind of preparation method preventing plant pest Natural Circulation microbial bacterial agent in addition.
To achieve these goals, the present invention proposes following technical scheme:
A kind of prevention plant pest Natural Circulation microbial bacterial agent, this microbial bacterial agent by dodging Bacillus (Allochromatium), Lamorocystis (Lamprocystis), belong to (Nitrobacter) addicted to the red spiral Pseudomonas (Halorhodospira) of salt, Chromatium (Chromatium), Nitrosomonas (Nitrosomonas), nitrate bacteria, the zymotic fluid of Rhodospirillum (Rhodospirillum) 7 kinds of bacteriums forms, the living bacteria count of above 7 kinds of bacteriums in zymotic fluid divides not all≤1.0 × 10
8cFU/ml.
Preferably, described sudden strain of a muscle Bacillus (Allochromatium) is dodge bacillus (Lamprobactermodestohalophilus) addicted to middle salt; Described Lamorocystis (Lamprocystis) is pink sudden strain of a muscle capsule bacterium (Lamprocystisroseopersicina); Described is addicted to the red spirillum of salt (Halorhodospirahalophila) addicted to salt addicted to salt red spiral Pseudomonas (Halorhodospira); Described Chromatium (Chromatium) is Ovshinsky chomophoric bacterium (Chromatiumokenii) or Chromatium vinosum (Chromatiumvinosum); Described Nitrosomonas (Nitrosomonas) is Mtrosomonas hair bacillus (Nitrosomonaseuropaea); Described nitrate bacteria genus (Nitrobacter) is for Vickers nitrate bacteria (Nitrobacterwinogradskyi) or enliven nitrate bacteria (N.agilis); Described Rhodospirillum (Rhodospirillum) is Rhodospirillum rubrum (Rhodospirillumrubrum).
Preferably, the medium that described zymotic fluid is used is add 0.5g potassium dihydrogen phosphate (KH in every 1L water
2pO
4), 0.2g epsom salt (MgSO
47H
2o), 0.4g sodium chloride (NaCI), 1.2g ammonium chloride (NH
4cI), 0.05g CALCIUM CHLORIDE DIHYDRATE (CaCI
22H
2o), 0.3g yeast extract (yeastextract), 1ml ore deposit liquid (containing 25%(v/v in the every 1L of mineralsolution() hydrogen chloride (HCI) 1ml, zinc chloride (ZnCI
2) 70mg, tetrahydrate manganese chloride (MnCI
24H
2o) 100mg, boric acid (H
3bO
3) 60mg, cobalt chloride (CoCI
26H
2o) 200mg, copper chloride (CuCI
2h
2o) 20mg, sodium molybdate (NaMoO
42H
2o) 40mg)) fully dissolve after, the PH of the aqueous solution is adjusted to 7.0, carries out autoclaving process.
The preparation method of prevention plant pest Natural Circulation microbial bacterial agent of the present invention, step is as follows:
(1) manufacture of medium
The manufacture method of this culture fluid is as follows, adds 0.5g potassium dihydrogen phosphate (KH in every 1L water
2pO
4), 0.2g epsom salt (MgSO
47H
2o), 0.4g sodium chloride (NaCI), 1.2g ammonium chloride (NH
4cI), 0.05g CALCIUM CHLORIDE DIHYDRATE (CaCI
22H
2o), 0.3g yeast extract (yeastextract), 1ml ore deposit liquid (containing 25%(v/v in the every 1L of mineralsolution() hydrogen chloride (HCI) 1ml, zinc chloride (ZnCI
2) 70mg, tetrahydrate manganese chloride (MnCI
24H
2o) 100mg, boric acid (H
3bO
3) 60mg, cobalt chloride (CoCI
26H
2o) 200mg, copper chloride (CuCI
2h
2o) 20mg, sodium molybdate (NaMoO
42H
2o) 40mg)) fully dissolve after, the PH of the aqueous solution is adjusted to 7.0, carries out autoclaving process.
(2) benchmark is cultivated
The former bacterium of medium 5L above, 7 kinds of equivalent is put into the transparent glass container that 100L water is housed and maintains the temperature of water at 36-40 DEG C, distance water surface 12-18cm 4 36w tri-wavelength bulbs throw light on, and cultivate 6-8 days.
In said method, preferably, in described step (2), the temperature of water is 38 DEG C, and distance water surface 15cm, cultivates 7 days.
In said method, preferably, the former bacterium of putting into described in be 7 kinds of bacterial strains after step (1) described medium culture through detect bacterium colony quantity≤1.0 × 10
8bacterium liquid after CFU/ml, the bacterium liquid 5L altogether of 7 kinds of bacterium pearls.
The purposes of microbial bacterial agent of the present invention and the scope of application
The usage amount of microbial bacterial agent of the present invention and using method
Above-mentioned usage amount has difference significantly according to place difference.Microbial bacterial agent of the present invention has good effect to each Plants prevention disease and growth promoting effects.
Additional aspect of the present invention and advantage will part provide in the following description, and part will become obvious from the following description, or be recognized by practice of the present invention.
Embodiment
Below by embodiment, technical scheme of the present invention is described further, but can not limitation of the present invention be interpreted as.
embodiment 1
For preventing a Natural Circulation microbial bacterial agent for plant pest, quality benchmark:
Through experiment, bacterial strain involved in the present invention is known bacterial strain, and adopts the microbial inoculum of this bacterium pearl all to have identical effects of purification quality.
The preparation method of described prevention plant pest Natural Circulation microbial bacterial agent, step is as follows:
(1) manufacture method of medium
The manufacture method of this culture fluid is as follows, adds 0.5g potassium dihydrogen phosphate (KH in every 1L water
2pO
4), 0.2g epsom salt (MgSO
47H
2o), 0.4g sodium chloride (NaCI), 1.2g ammonium chloride (NH
4cI), 0.05g CALCIUM CHLORIDE DIHYDRATE (CaCI
22H
2o), 0.3g yeast extract, 1ml ore deposit liquid (containing 25%(v/v in the every 1L of mineralsolution() hydrogen chloride (HCI) 1ml, zinc chloride (ZnCI
2) 70mg, tetrahydrate manganese chloride (MnCI
24H
2o) 100mg, boric acid (H
3bO
3) 60mg, cobalt chloride (CoCI
26H
2o) 200mg, copper chloride (CuCI
2h
2o) 20mg, sodium molybdate (NaMoO
42H
2o) 40mg)) fully dissolve after, the PH of the aqueous solution is adjusted to 7.0, carries out autoclaving process.
(2) benchmark is cultivated
Medium 5L above, former bacterium 5L are put into the transparent glass container (aquarium) that 100L water is housed and maintain the temperature of water at about 38 DEG C, the distance water surface is about 15cm 4 36w tri-wavelength bulbs and throws light on, and cultivates about 7 days.
experimental example 1: effect
In the 4-9 month in 2013,6 totally months periods tested with regard to spinach, Chinese cabbage, radish respectively in growing vegetables base, Weifang.Experimental group is by by the method for weekly ground of plant being sprayed to (spinach and Chinese cabbage) or root perfusion (radish) after carry out 100 times of dilutions with water with upper part.Control group does not use this formula.Relative to control group, spinach group and radish group disease obviously reduce, and resistance strengthens, and increases production about 18%(weight); Chinese cabbage group leader gesture is better.
detection reference
(1) get that above-mentioned the said medium of 1L adds that 1-3g agar (agar) stirs, after autoclaving (autoclave), be placed in culture dish (petridish).
(2) in the culture dish solidified, evenly spray the Natural Circulation microbial bacterial agent after 100 times or 1000 times of dilutions.
(3) the top 36w tri-wavelength bulb light of distance culture dish 10cm, maintains the temperature at about 38 DEG C, and cultivating after about 7 days can the colony counts of bacterial detection.
(4) often kind of bacterial clump quantity all>=1.0 × 10
8cFU/ml.
Claims (5)
1. a prevention plant pest Natural Circulation microbial bacterial agent, it is characterized in that, this microbial bacterial agent is by dodging Bacillus (Allochromatium), Lamorocystis (Lamprocystis), forming addicted to the zymotic fluid of the red spiral Pseudomonas (Halorhodospira) of salt, Chromatium (Chromatium), Nitrosomonas (Nitrosomonas), nitrate bacteria genus (Nitrobacter), Rhodospirillum (Rhodospirillum) 7 kinds of bacteriums, and the living bacteria count of above 7 kinds of bacteriums in zymotic fluid distinguishes all>=1.0 × 10
8cFU/ml;
Described zymotic fluid medium used is add 0.5g potassium dihydrogen phosphate (KH in every 1L water
2pO
4), 0.2g epsom salt (MgSO
47H
2o), 0.4g sodium chloride (NaCl), 1.2g ammonium chloride (NH
4cl), 0.05g CALCIUM CHLORIDE DIHYDRATE (CaCl
22H
2o), 0.3g yeast extract, 1ml ore deposit liquid (mineralsolution), containing 25%(v/v in the liquid of every 1L ore deposit) hydrogen chloride (HCl) 1ml, zinc chloride (ZnCl
2) 70mg, tetrahydrate manganese chloride (MnCl
24H
2o) 100mg, boric acid (H
3bO
3) 60mg, cobalt chloride hexahydrate (CoCl
26H
2o) 200mg, a Hydrated copper chloride (CuCl
2h
2o) 20mg, two molybdic acid hydrate sodium (NaMoO
42H
2o) 40mg, after fully dissolving, the PH of the aqueous solution is adjusted to 7.0, carries out autoclaving process.
2. prevention plant pest Natural Circulation microbial bacterial agent according to claim 1, is characterized in that, described sudden strain of a muscle Bacillus (Allochromatium) is dodge bacillus (Lamprobactermodestohalophilus) addicted to middle salt; Described Lamorocystis (Lamprocystis) is pink sudden strain of a muscle capsule bacterium (Lamprocystisroseopersicina); Described is addicted to the red spirillum of salt (Halorhodospirahalophila) addicted to salt addicted to salt red spiral Pseudomonas (Halorhodospira); Described Chromatium (Chromatium) is Ovshinsky chomophoric bacterium (Chromatiumokenii) or Chromatium vinosum (Chromatiumvinosum); Described Nitrosomonas (Nitrosomonas) is Mtrosomonas hair bacillus (Nitrosomonaseuropaea); Described nitrate bacteria genus (Nitrobacter) is for Vickers nitrate bacteria (Nitrobacterwinogradskyi) or enliven nitrate bacteria (N.agilis); Described Rhodospirillum (Rhodospirillum) is Rhodospirillum rubrum (Rhodospirillumrubrum).
3. the prevention plant pest preparation method of Natural Circulation microbial bacterial agent described in any one of claim 1-2, it is characterized in that, step is as follows:
(1) manufacture of medium
The manufacture method of this culture fluid is as follows, adds 0.5g potassium dihydrogen phosphate (KH in every 1L water
2pO
4), 0.2g epsom salt (MgSO
47H
2o), 0.4g sodium chloride (NaCl), 1.2g ammonium chloride (NH
4cl), 0.05g CALCIUM CHLORIDE DIHYDRATE (CaCl
22H
2o), 0.3g yeast extract, 1ml ore deposit liquid (mineralsolution), containing 25%(v/v in the liquid of every 1L ore deposit) hydrogen chloride (HCl) 1ml, zinc chloride (ZnCl
2) 70mg, tetrahydrate manganese chloride (MnCl
24H
2o) 100mg, boric acid (H
3bO
3) 60mg, cobalt chloride hexahydrate (CoCl
26H
2o) 200mg, a Hydrated copper chloride (CuCl
2h
2o) 20mg, two molybdic acid hydrate sodium (NaMoO
42H
2o) 40mg, after fully dissolving, the PH of the aqueous solution is adjusted to 7.0, carries out autoclaving process;
(2) benchmark is cultivated
The former bacterium of medium 5L above, 7 kinds of equivalent is put into the transparent glass container that 100L water is housed and maintains the temperature of water at 36-40 DEG C, distance water surface 12-18cm 4 36w tri-wavelength bulbs throw light on, and cultivate 6-8 days.
4. method according to claim 3, is characterized in that, in described step (2), the temperature of water is 38 DEG C, and distance water surface 15cm, cultivates 7 days.
5. method according to claim 3, is characterized in that, described in the former bacterium of putting into be 7 kinds of bacterial strains after step (1) described medium culture through detecting colony counts>=1.0 × 10
8bacterium liquid after CFU/ml, the bacterium liquid 5L altogether of 7 kinds of bacterium pearls.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310472045.4A CN103843825B (en) | 2013-10-11 | 2013-10-11 | Prevention plant pest Natural Circulation microbial bacterial agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310472045.4A CN103843825B (en) | 2013-10-11 | 2013-10-11 | Prevention plant pest Natural Circulation microbial bacterial agent and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103843825A CN103843825A (en) | 2014-06-11 |
| CN103843825B true CN103843825B (en) | 2016-04-27 |
Family
ID=50852283
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310472045.4A Active CN103843825B (en) | 2013-10-11 | 2013-10-11 | Prevention plant pest Natural Circulation microbial bacterial agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103843825B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108947673A (en) * | 2018-08-14 | 2018-12-07 | 侯希波 | A kind of soil improvement Natural Circulation microbial bacterial agent and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86103258A (en) * | 1985-12-31 | 1987-07-08 | 戈登·李·威廉斯 | Microbial fertilizer and soil improvement agent |
| CN1602403A (en) * | 2001-10-12 | 2005-03-30 | 索拉诺公司 | Solar collector plate, method for safeguarding the operation of a solar collector, and method for manufacturing a solar collector plate |
| CN1733896A (en) * | 2005-07-29 | 2006-02-15 | 齐进军 | Bacteria for improving agricultural production environment and the quality of products and yield, and its preparation method |
| CN102191178A (en) * | 2011-03-25 | 2011-09-21 | 江苏碧程环保设备有限公司 | Compound microbial inoculum and preparation method thereof |
| WO2013148278A1 (en) * | 2012-03-27 | 2013-10-03 | Agrinos AS | Microbial composition comprising liquid fertilizer and processes for agricultural use |
-
2013
- 2013-10-11 CN CN201310472045.4A patent/CN103843825B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86103258A (en) * | 1985-12-31 | 1987-07-08 | 戈登·李·威廉斯 | Microbial fertilizer and soil improvement agent |
| CN1602403A (en) * | 2001-10-12 | 2005-03-30 | 索拉诺公司 | Solar collector plate, method for safeguarding the operation of a solar collector, and method for manufacturing a solar collector plate |
| CN1733896A (en) * | 2005-07-29 | 2006-02-15 | 齐进军 | Bacteria for improving agricultural production environment and the quality of products and yield, and its preparation method |
| CN102191178A (en) * | 2011-03-25 | 2011-09-21 | 江苏碧程环保设备有限公司 | Compound microbial inoculum and preparation method thereof |
| WO2013148278A1 (en) * | 2012-03-27 | 2013-10-03 | Agrinos AS | Microbial composition comprising liquid fertilizer and processes for agricultural use |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103843825A (en) | 2014-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Padisak et al. | Phytoplankton in extreme environments: importance and consequences of habitat permanency | |
| CN103224886B (en) | Composite microbe agent for aquatic product culture and application thereof | |
| Matsudo et al. | Repeated fed-batch cultivation of Arthrospira (Spirulina) platensis using urea as nitrogen source | |
| Rahim | Application of seaweed Gracilaria verrucosa tissue culture using different doses of vermicompost fertilizer | |
| Rodrigues et al. | Influence of ammonium sulphate feeding time on fed-batch Arthrospira (Spirulina) platensis cultivation and biomass composition with and without pH control | |
| CN104059855B (en) | Composite fungicide for treating heavy metal pollution of soil and preparation method of composite fungicide | |
| CN108913680A (en) | A kind of microorganism formulation and its application for cultivation water environment ammonia nitrogen degradation | |
| CN101759491A (en) | Bacillus subtilis suspended seed coating agent, preparation method and application thereof | |
| CN102745796A (en) | Bioremediation treatment process for surface water with water blooms | |
| CN105316237B (en) | A kind of chlorella efficiently cultivates the composition of culture solution | |
| JP2013256488A (en) | Plant disease controlling agent and method for controlling plant disease using the same | |
| CN103843825B (en) | Prevention plant pest Natural Circulation microbial bacterial agent and preparation method thereof | |
| CN106010969A (en) | Large-scale cultivation method of flagellate ochromonas sp swallowing microcystis | |
| CN106430363A (en) | Water purifying agent for pond | |
| CN105132332B (en) | One strain of gluconacetobacter and its application as plant growth-promoting bacteria | |
| CN105385642A (en) | Brevundimonas and application thereof | |
| CN105861395B (en) | It is a kind of it is effective inhibit wawter bloom microcystic aeruginosa composite bacteria agent DH-1 and its application | |
| CN105217799A (en) | A kind of industrial fermentation method of molten algae streptomycete active substance | |
| CN107473405A (en) | A kind of method and device that blue-green alga bloom is controlled using lactic acid bacteria | |
| TWI464260B (en) | Novel isolated purple non-sulfur photosynthetic bacteria and use and screen method thereof | |
| CN103849579B (en) | Purify water with Natural Circulation microbial bacterial agent and preparation method thereof | |
| CN102352319A (en) | Culture method for accumulating organic iodine in Dunaliella salina | |
| CN102626111B (en) | Preparation method of immune transpiration inhibitor based on closed stomatal induction chlorella vulgaris | |
| CN107493876B (en) | The method and application of PAEs pollution in plant are reduced using endophyte of plant | |
| CN103849578B (en) | Purify air and remove stench Natural Circulation microbial bacterial agent and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: Hou Xibo Inventor before: Hou Xibo Inventor before: Li Jun |
|
| COR | Change of bibliographic data | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210804 Address after: 262734 No. 345, Chenming Road, Shouguang City, Weifang City, Shandong Province Patentee after: Hou Xibo Address before: 26273 Dongcheng Industrial Park, Shouguang City, Weifang City, Shandong Province Patentee before: SHANDONG XINKAI BIOTECH Co.,Ltd. |
|
| TR01 | Transfer of patent right |