CN103843825A - Natural circulating microbial agent for preventing plant diseases and insect pests and preparation method thereof - Google Patents
Natural circulating microbial agent for preventing plant diseases and insect pests and preparation method thereof Download PDFInfo
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- CN103843825A CN103843825A CN201310472045.4A CN201310472045A CN103843825A CN 103843825 A CN103843825 A CN 103843825A CN 201310472045 A CN201310472045 A CN 201310472045A CN 103843825 A CN103843825 A CN 103843825A
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- chloride
- chromatium
- bacterium
- rhodospirillum
- salt
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 25
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 15
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 201000010099 disease Diseases 0.000 title abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract description 4
- 241000238631 Hexapoda Species 0.000 title abstract 2
- 241000894006 Bacteria Species 0.000 claims abstract description 32
- 241000190831 Chromatium Species 0.000 claims abstract description 10
- 241000605122 Nitrosomonas Species 0.000 claims abstract description 10
- 241000190967 Rhodospirillum Species 0.000 claims abstract description 10
- 241001655243 Allochromatium Species 0.000 claims abstract description 6
- 241001655879 Halorhodospira Species 0.000 claims abstract description 6
- 241001353956 Lamprocystis <gastropod> Species 0.000 claims abstract description 6
- 241000605159 Nitrobacter Species 0.000 claims abstract description 6
- 230000001580 bacterial effect Effects 0.000 claims description 26
- 239000003795 chemical substances by application Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 241000196324 Embryophyta Species 0.000 claims description 15
- 230000002265 prevention Effects 0.000 claims description 11
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 229910000041 hydrogen chloride Inorganic materials 0.000 claims description 10
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 150000003839 salts Chemical class 0.000 claims description 10
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 229910002651 NO3 Inorganic materials 0.000 claims description 8
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 7
- 239000012138 yeast extract Substances 0.000 claims description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 6
- 235000019270 ammonium chloride Nutrition 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 5
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 claims description 5
- 229940052299 calcium chloride dihydrate Drugs 0.000 claims description 5
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 5
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 claims description 5
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 claims description 5
- 235000010755 mineral Nutrition 0.000 claims description 5
- 239000011707 mineral Substances 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000011684 sodium molybdate Substances 0.000 claims description 5
- 235000015393 sodium molybdate Nutrition 0.000 claims description 5
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 5
- 239000011592 zinc chloride Substances 0.000 claims description 5
- 235000005074 zinc chloride Nutrition 0.000 claims description 5
- 241000190857 Allochromatium vinosum Species 0.000 claims description 4
- 241000605154 Nitrobacter winogradskyi Species 0.000 claims description 4
- 241000589516 Pseudomonas Species 0.000 claims description 4
- 241000190984 Rhodospirillum rubrum Species 0.000 claims description 4
- 239000012531 culture fluid Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000011049 pearl Substances 0.000 claims description 3
- 241000881804 Chromatium okenii Species 0.000 claims description 2
- 241000191005 Halorhodospira halophila Species 0.000 claims description 2
- 241000420933 Lamprobacter modestohalophilus Species 0.000 claims description 2
- 241000520773 Lamprocystis roseopersicina Species 0.000 claims description 2
- 241000605121 Nitrosomonas europaea Species 0.000 claims description 2
- 241000605008 Spirillum Species 0.000 claims description 2
- 239000002775 capsule Substances 0.000 claims description 2
- 210000003205 muscle Anatomy 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 abstract 2
- 230000004151 fermentation Effects 0.000 abstract 2
- 230000006806 disease prevention Effects 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 3
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 3
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 3
- 244000088415 Raphanus sativus Species 0.000 description 3
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 3
- 241000219315 Spinacia Species 0.000 description 3
- 235000009337 Spinacia oleracea Nutrition 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a natural circulating microbial agent for preventing plant diseases and insect pests and a preparation method thereof. The microbial agent is formed by fermentation liquor of seven bacteria including allochromatium (allochromatium), lamprocystis (lamprocystis), halorhodospira (halorhodospira), chromatium (chromatium), nitrosomonas (nitrosomonas), nitrobacter (nitrobacter) and rhodospirillum (rhodospirillum), wherein the valid viable counts of the seven bacteria in the fermentation liquor respectively are greater than or equal to 1.0*10<8>CFU/ml. The microbial agent disclosed by the invention has good action on prevention of diseases on kinds of plants and promoting growth.
Description
Technical field
The invention belongs to environmental technology field, relate to a kind of microbial bacterial agent, be specifically related to a kind of prevention plant pest Natural Circulation microbial bacterial agent and preparation method.
Background technology
Closely for decades, develop rapidly and brought severe environmental pollution and ecological disruption with science and technology, social economy.The great potential of microbial degradation pollutant plays a significant role in control pollution, repairing environment.Sewage (waste water), waste gas and solid waste that human being's production and life activity produce can be processed with biological method., in technology, also lack a kind of for preventing the microbial bacterial agent of plant pest now.
Summary of the invention
Object of the present invention is exactly for the deficiencies in the prior art, and a kind of prevention plant pest Natural Circulation microbial bacterial agent is provided.
The present invention provides a kind of preparation method who prevents plant pest Natural Circulation microbial bacterial agent in addition.
To achieve these goals, the present invention proposes following technical scheme:
A kind of prevention plant pest Natural Circulation microbial bacterial agent, this microbial bacterial agent by dodging Bacillus (Allochromatium), Lamorocystis (Lamprocystis), have a liking for the red spiral Pseudomonas of salt (Halorhodospira), zymotic fluid that Chromatium (Chromatium), Nitrosomonas (Nitrosomonas), nitrate bacteria belong to (Nitrobacter), 7 kinds of bacteriums of Rhodospirillum (Rhodospirillum) form, the living bacteria count of above 7 kinds of bacteriums in zymotic fluid distinguishes all≤1.0 × 10
8cFU/ml.
Preferably, described sudden strain of a muscle Bacillus (Allochromatium) dodges bacillus (Lamprobacter modestohalophilus) for having a liking for middle salt; Described Lamorocystis (Lamprocystis) is the pink capsule bacterium (Lamprocystis roseopersicina) that dodges; Describedly have a liking for the red spiral Pseudomonas of salt (Halorhodospira) and have a liking for the red spirillum of salt (Halorhodospira halophila) for having a liking for salt; Described Chromatium (Chromatium) is Ovshinsky chomophoric bacterium (Chromatium okenii) or Chromatium vinosum (Chromatium vinosum); Described Nitrosomonas (Nitrosomonas) is European nitrosomonus (Nitrosomonas europaea); Described nitrate bacteria belongs to (Nitrobacter) for Vickers nitrate bacteria (Nitrobacter winogradskyi) or enlivens nitrate bacteria (N. agilis); Described Rhodospirillum (Rhodospirillum) is Rhodospirillum rubrum (Rhodospirillum rubrum).
Preferably, described zymotic fluid medium used is in every 1L water, to add 0.5g potassium dihydrogen phosphate (KH
2pO
4), 0.2g epsom salt (MgSO
47H
2o), 0.4g sodium chloride (NaCI), 1.2g ammonium chloride (NH
4cI), 0.05g CALCIUM CHLORIDE DIHYDRATE (CaCI
22H
2o), 0.3g yeast extract (yeast extract), 1ml ore deposit liquid (containing 25%(v/v in the every 1L of mineral solution() hydrogen chloride (HCI) 1ml, zinc chloride (ZnCI
2) 70mg, tetrahydrate manganese chloride (MnCI
24H
2o) 100mg, boric acid (H
3bO
3) 60mg, cobalt chloride (CoCI
26H
2o) 200mg, copper chloride (CuCI
2h
2o) 20mg, sodium molybdate (NaMoO
42H
2o) 40mg)) fully dissolve after, the PH of the aqueous solution is adjusted to 7.0, carries out autoclaving processing.
The preparation method of Natural Circulation microbial bacterial agent for prevention plant pest of the present invention, step is as follows:
(1) manufacture of medium
The manufacture method of this culture fluid is as follows, adds 0.5g potassium dihydrogen phosphate (KH in every 1L water
2pO
4), 0.2g epsom salt (MgSO
47H
2o), 0.4g sodium chloride (NaCI), 1.2g ammonium chloride (NH
4cI), 0.05g CALCIUM CHLORIDE DIHYDRATE (CaCI
22H
2o), 0.3g yeast extract (yeast extract), 1ml ore deposit liquid (containing 25%(v/v in the every 1L of mineral solution() hydrogen chloride (HCI) 1ml, zinc chloride (ZnCI
2) 70mg, tetrahydrate manganese chloride (MnCI
24H
2o) 100mg, boric acid (H
3bO
3) 60mg, cobalt chloride (CoCI
26H
2o) 200mg, copper chloride (CuCI
2h
2o) 20mg, sodium molybdate (NaMoO
42H
2o) 40mg)) fully dissolve after, the PH of the aqueous solution is adjusted to 7.0, carries out autoclaving processing.
(2) cultivate benchmark
The former bacterium of medium 5L above, 7 kinds of equivalent is put into and temperature that the transparent glass container of 100L water maintains water is housed at 36-40 ℃, throw light on 4 36w three-wavelength bulbs apart from water surface 12-18cm, cultivate 6-8 days.
In said method, preferred, in described step (2), the temperature of water is 38 ℃, apart from water surface 15cm, cultivates 7 days.
In said method, preferred, described in the former bacterium of putting into be 7 kinds of bacterial strains after the described medium culture of step (1) through detecting bacterium colony quantity≤1.0 × 10
8bacterium liquid after CFU/ml, the bacterium liquid of 7 kinds of bacterium pearls is 5L altogether.
The purposes of microbial bacterial agent of the present invention and the scope of application
The usage amount of microbial bacterial agent of the present invention and using method
Above-mentioned usage amount has difference significantly according to place difference.Microbial bacterial agent of the present invention is to each Plants prevention disease and promote growth to have good effect.
Additional aspect of the present invention and advantage in the following description part provide, and part will become obviously from the following description, or recognize by practice of the present invention.
Embodiment
Below by embodiment, technical scheme of the present invention is described further, but can not be interpreted as limitation of the present invention.
embodiment 1
For preventing a Natural Circulation microbial bacterial agent for plant pest, quality benchmark:
Through experiment, bacterial strain involved in the present invention is known bacterial strain, and adopts the microbial inoculum of this bacterium pearl all to have identical effects of purification quality.
The preparation method of Natural Circulation microbial bacterial agent for described prevention plant pest, step is as follows:
(1) manufacture method of medium
The manufacture method of this culture fluid is as follows, adds 0.5g potassium dihydrogen phosphate (KH in every 1L water
2pO
4), 0.2g epsom salt (MgSO
47H
2o), 0.4g sodium chloride (NaCI), 1.2g ammonium chloride (NH
4cI), 0.05g CALCIUM CHLORIDE DIHYDRATE (CaCI
22H
2o), 0.3g yeast extract, 1ml ore deposit liquid (containing 25%(v/v in the every 1L of mineral solution() hydrogen chloride (HCI) 1ml, zinc chloride (ZnCI
2) 70mg, tetrahydrate manganese chloride (MnCI
24H
2o) 100mg, boric acid (H
3bO
3) 60mg, cobalt chloride (CoCI
26H
2o) 200mg, copper chloride (CuCI
2h
2o) 20mg, sodium molybdate (NaMoO
42H
2o) 40mg)) fully dissolve after, the PH of the aqueous solution is adjusted to 7.0, carries out autoclaving processing.
(2) cultivate benchmark
Medium 5L above, former bacterium 5L are put into and temperature that the transparent glass container (aquarium) of 100L water maintains water be housed at approximately 38 ℃, throw light on 4 36w three-wavelength bulbs apart from the about 15cm of the water surface, cultivate approximately 7 days.
experimental example 1: effect
Test with regard to spinach, Chinese cabbage, radish respectively in growing vegetables base, Weifang during 6 totally months in the 4-9 month in 2013.Experimental group is by carrying out 100 times of dilutions with water later weekly to the above method of partly spraying (spinach and Chinese cabbage) or root perfusion (radish) in the ground of plant.Control group is not used this formula.With respect to control group, spinach group and radish group disease obviously reduce, and resistance strengthens, and increases production about 18%(weight); Chinese cabbage group leader gesture is better.
detection reference
(1) get that medium that 1L is above-mentioned said adds that 1-3g agar (agar) stirs, after autoclaving (autoclave), be placed in culture dish (petridish).
(2) the Natural Circulation microbial bacterial agent after 100 times of even sprinklings or 1000 times of dilutions in the culture dish solidifying.
(3) apart from 36w three-wavelength bulb illumination for the top of culture dish 10cm, maintain the temperature at approximately 38 ℃, cultivate after approximately 7 days can bacterial detection colony counts.
(4) every kind of bacterial clump quantity all>=1.0 × 10
8cFU/ml.
Claims (6)
1. a prevention plant pest Natural Circulation microbial bacterial agent, it is characterized in that, this microbial bacterial agent by dodging Bacillus (Allochromatium), Lamorocystis (Lamprocystis), have a liking for the red spiral Pseudomonas of salt (Halorhodospira), zymotic fluid that Chromatium (Chromatium), Nitrosomonas (Nitrosomonas), nitrate bacteria belong to (Nitrobacter), 7 kinds of bacteriums of Rhodospirillum (Rhodospirillum) form, the living bacteria count of above 7 kinds of bacteriums in zymotic fluid distinguishes all≤1.0 × 10
8cFU/ml.
2. prevention plant pest Natural Circulation microbial bacterial agent according to claim 1, is characterized in that, described sudden strain of a muscle Bacillus (Allochromatium) dodges bacillus (Lamprobacter modestohalophilus) for having a liking for middle salt; Described Lamorocystis (Lamprocystis) is the pink capsule bacterium (Lamprocystis roseopersicina) that dodges; Describedly have a liking for the red spiral Pseudomonas of salt (Halorhodospira) and have a liking for the red spirillum of salt (Halorhodospira halophila) for having a liking for salt; Described Chromatium (Chromatium) is Ovshinsky chomophoric bacterium (Chromatium okenii) or Chromatium vinosum (Chromatium vinosum); Described Nitrosomonas (Nitrosomonas) is European nitrosomonus (Nitrosomonas europaea); Described nitrate bacteria belongs to (Nitrobacter) for Vickers nitrate bacteria (Nitrobacter winogradskyi) or enlivens nitrate bacteria (N. agilis); Described Rhodospirillum (Rhodospirillum) is Rhodospirillum rubrum (Rhodospirillum rubrum).
3. according to claim 1 or described prevention plant pest Natural Circulation microbial bacterial agent, it is characterized in that, described zymotic fluid medium used is in every 1L water, to add 0.5g potassium dihydrogen phosphate (KH
2pO
4), 0.2g epsom salt (MgSO
47H
2o), 0.4g sodium chloride (NaCI), 1.2g ammonium chloride (NH
4cI), 0.05g CALCIUM CHLORIDE DIHYDRATE (CaCI
22H
2o), 0.3g yeast extract, 1ml ore deposit liquid (containing 25%(v/v in the every 1L of mineral solution() hydrogen chloride (HCI) 1ml, zinc chloride (ZnCI
2) 70mg, tetrahydrate manganese chloride (MnCI
24H
2o) 100mg, boric acid (H
3bO
3) 60mg, cobalt chloride (CoCI
26H
2o) 200mg, copper chloride (CuCI
2h
2o) 20mg, sodium molybdate (NaMoO
42H
2o) 40mg)) fully dissolve after, the PH of the aqueous solution is adjusted to 7.0, carries out autoclaving processing.
4. the preparation method of Natural Circulation microbial bacterial agent for the prevention plant pest described in claim 1-3 any one, is characterized in that, step is as follows:
(1) manufacture of medium
The manufacture method of this culture fluid is as follows, adds 0.5g potassium dihydrogen phosphate (KH in every 1L water
2pO
4), 0.2g epsom salt (MgSO
47H
2o), 0.4g sodium chloride (NaCI), 1.2g ammonium chloride (NH
4cI), 0.05g CALCIUM CHLORIDE DIHYDRATE (CaCI
22H
2o), 0.3g yeast extract, 1ml ore deposit liquid (containing 25%(v/v in the every 1L of mineral solution() hydrogen chloride (HCI) 1ml, zinc chloride (ZnCI
2) 70mg, tetrahydrate manganese chloride (MnCI
24H
2o) 100mg, boric acid (H
3bO
3) 60mg, cobalt chloride (CoCI
26H
2o) 200mg, copper chloride (CuCI
2h
2o) 20mg, sodium molybdate (NaMoO
42H
2o) 40mg)) fully dissolve after, the PH of the aqueous solution is adjusted to 7.0, carries out autoclaving processing;
(2) cultivate benchmark
The former bacterium of medium 5L above, 7 kinds of equivalent is put into and temperature that the transparent glass container of 100L water maintains water is housed at 36-40 ℃, throw light on 4 36w three-wavelength bulbs apart from water surface 12-18cm, cultivate 6-8 days.
5. method according to claim 4, is characterized in that, in described step (2), the temperature of water is 38 ℃, apart from water surface 15cm, cultivates 7 days.
6. method according to claim 4, is characterized in that, described in the former bacterium of putting into be 7 kinds of bacterial strains after the described medium culture of step (1) through detecting bacterium colony quantity≤1.0 × 10
8bacterium liquid after CFU/ml, the bacterium liquid of 7 kinds of bacterium pearls is 5L altogether.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310472045.4A CN103843825B (en) | 2013-10-11 | 2013-10-11 | Prevention plant pest Natural Circulation microbial bacterial agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310472045.4A CN103843825B (en) | 2013-10-11 | 2013-10-11 | Prevention plant pest Natural Circulation microbial bacterial agent and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103843825A true CN103843825A (en) | 2014-06-11 |
| CN103843825B CN103843825B (en) | 2016-04-27 |
Family
ID=50852283
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310472045.4A Active CN103843825B (en) | 2013-10-11 | 2013-10-11 | Prevention plant pest Natural Circulation microbial bacterial agent and preparation method thereof |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108947673A (en) * | 2018-08-14 | 2018-12-07 | 侯希波 | A kind of soil improvement Natural Circulation microbial bacterial agent and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86103258A (en) * | 1985-12-31 | 1987-07-08 | 戈登·李·威廉斯 | Microbial fertilizer and soil improvement agent |
| CN1602403A (en) * | 2001-10-12 | 2005-03-30 | 索拉诺公司 | Solar collector plate, method for safeguarding the operation of a solar collector, and method for manufacturing a solar collector plate |
| CN1733896A (en) * | 2005-07-29 | 2006-02-15 | 齐进军 | Bacteria for improving agricultural production environment and the quality of products and yield, and its preparation method |
| CN102191178A (en) * | 2011-03-25 | 2011-09-21 | 江苏碧程环保设备有限公司 | Compound microbial inoculum and preparation method thereof |
| WO2013148278A1 (en) * | 2012-03-27 | 2013-10-03 | Agrinos AS | Microbial composition comprising liquid fertilizer and processes for agricultural use |
-
2013
- 2013-10-11 CN CN201310472045.4A patent/CN103843825B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86103258A (en) * | 1985-12-31 | 1987-07-08 | 戈登·李·威廉斯 | Microbial fertilizer and soil improvement agent |
| CN1602403A (en) * | 2001-10-12 | 2005-03-30 | 索拉诺公司 | Solar collector plate, method for safeguarding the operation of a solar collector, and method for manufacturing a solar collector plate |
| CN1733896A (en) * | 2005-07-29 | 2006-02-15 | 齐进军 | Bacteria for improving agricultural production environment and the quality of products and yield, and its preparation method |
| CN102191178A (en) * | 2011-03-25 | 2011-09-21 | 江苏碧程环保设备有限公司 | Compound microbial inoculum and preparation method thereof |
| WO2013148278A1 (en) * | 2012-03-27 | 2013-10-03 | Agrinos AS | Microbial composition comprising liquid fertilizer and processes for agricultural use |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108947673A (en) * | 2018-08-14 | 2018-12-07 | 侯希波 | A kind of soil improvement Natural Circulation microbial bacterial agent and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103843825B (en) | 2016-04-27 |
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