CN107176751B - Emergency disposal method for rapidly reducing water bloom hazard of freshwater fish culture pond - Google Patents
Emergency disposal method for rapidly reducing water bloom hazard of freshwater fish culture pond Download PDFInfo
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- CN107176751B CN107176751B CN201710379741.9A CN201710379741A CN107176751B CN 107176751 B CN107176751 B CN 107176751B CN 201710379741 A CN201710379741 A CN 201710379741A CN 107176751 B CN107176751 B CN 107176751B
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- 229960005080 warfarin Drugs 0.000 description 2
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- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- -1 CaCO)30.1 g Chemical compound 0.000 description 1
- 241001609213 Carassius carassius Species 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/40—Devices for separating or removing fatty or oily substances or similar floating material
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F7/00—Aeration of stretches of water
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Farming Of Fish And Shellfish (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an emergency disposal method for rapidly reducing the hazard of the bloom of a freshwater fish culture pond, which adopts a physical method to drive most of the bloom of blue algae in the freshwater fish culture pond to a downwind port, and forms a similar enclosure area after a purse net; and (3) splashing a biological flocculant to the similar enclosure area, then fishing out the blue algae flocs, then adding a slow-release microcystin degradation catalyst, and finally splashing a fish antidote taking vitamin C as a main component to the culture pond. When the cyanobacterial bloom in the freshwater fish culture pond is treated in an emergency manner, the method can remarkably reduce the biomass of the cyanobacterial bloom in the culture water body in a very short time, effectively catalyze, degrade and remove microcystin in the culture water body, enhance the immunity of cultured fishes, and further reduce the harm of the cyanobacterial bloom to the cultured fishes fundamentally. The method is efficient and good in ecological safety, can greatly reduce the harm of the pond cyanobacterial bloom to the cultured fishes in a short time, does not cause secondary damage to the cultured fishes, and can be applied to emergency treatment of the cyanobacterial bloom in the freshwater culture pond in a large scale.
Description
Technical Field
The invention belongs to the technical field of water body pollution control, and particularly relates to an emergency disposal method for quickly reducing water bloom hazards of a freshwater fish culture pond.
Background
The high-density intensive aquaculture mode promotes the continuous aggravation of the eutrophication degree of the aquaculture water in China, and further causes the frequent occurrence of the cyanobacterial bloom mainly comprising microcystis in the aquaculture pond. The harm of the cyanobacterial bloom to freshwater aquaculture is mainly in the following 4 aspects: 1) the proliferation cycle of the microcystis is short, the stress resistance is strong, absolute advantages can be rapidly occupied in a culture ecological system, and the balance of the culture ecological system is damaged; 2) due to CO in daytime2The concentration is reduced, and the microcystis utilizes HCO3 -The increase in the amount leads to CO3 2-The content is increased to promote the pH value of the water body to be increased rapidly (to 9.5-10), and the DO content is greatly reduced and a large amount of CO is released due to the respiration of the microcystis at night2The pH value of the aquaculture water is rapidly reduced, the DO content is rapidly reduced, and the pH value is greatly fluctuated, so that the survival of animals is seriously threatened; 3) the microcystis bloom is accumulated on the surface layer of the water body, the transparency of the water body is reduced, the photosynthesis of other algae is hindered, the DO content of the lower water body is greatly reduced, and organic matters including decline blue algae at the bottom of the pond are subjected to anaerobic decomposition to generate a large amount of H2S and NH2OH toxic substances which can poison the cultured animals to different degrees; 4) the microcystis can produce primary hepatotoxin-microcystin with stable chemical property, and the toxin has strong toxic action on the growth, development and reproduction of aquatic animals.
The method for emergently disposing the cyanobacterial bloom in the culture pond comprises physical methods such as water changing and continuous increase of water power, chemical methods such as splashing of copper ion preparations, antibiotics and allelochemicals algistats, biological methods such as splashing of bacillus and EM composite bacteria, and antidote which takes sodium humate as a main component is splashed to the culture pond after treatment by the methods. However, the emergency disposal method for cyanobacterial bloom in the aquaculture pond has great limitations in practical application, for example, frequent water replacement for freshwater fish aquaculture ponds in resource areas such as north China cannot be completed, and meanwhile, the energy is greatly consumed by continuously increasing the hydrodynamic force of the ponds, so that the aquaculture cost is increased; chemical preparations such as copper ions and antibiotics are splashed, although the algae inhibiting effect is strong, the ecological balance of the aquaculture pond is easily destroyed, and the secondary pollution of the pond is easily caused; although the allelochemical algistat does not cause secondary pollution, the short-time killing of a large amount of microcystis can cause the content of microcystin in the aquaculture water to be greatly improved, and further seriously threatens the survival of the aquaculture animals; the blue algae bloom is treated by biological methods such as bacillus splashing, EM composite bacteria and the like in an emergency mode, so that the blue algae bloom is slow in action and difficult to take effect in a short time, and a good application effect is difficult to obtain. In addition, antidotes taking sodium humate as a main component are splashed into the aquaculture pond after the cyanobacterial bloom is treated in an emergency mode, a small amount of antidotes can adsorb soluble microcystins in aquaculture water, the antidotes have little effect, and meanwhile, the sodium humate cannot improve the immunity of aquaculture fishes, so that the aquaculture fishes die to different degrees after the cyanobacterial bloom is treated in an emergency mode by a chemical method (antibiotics and allelochemicals as algistats) and the like, and the economic benefit of aquaculture is seriously damaged.
Disclosure of Invention
The invention aims to provide an emergency disposal method for rapidly reducing the hazard of the algal bloom in the freshwater fish culture pond, which can be applied to the emergency disposal of the cyanobacteria bloom in the freshwater fish culture pond in a large scale.
In order to solve the technical problems, the invention adopts the technical scheme that: an emergency disposal method for rapidly reducing water bloom hazards of a freshwater fish culture pond comprises the following steps:
(a) opening an impeller type aerator in a freshwater fish culture pond which has burst cyanobacterial bloom for 30-60 minutes to force the cyanobacterial bloom to gather at the edge of the culture pond as much as possible, particularly around a lower tuyere of the culture pond;
(b) feeding a small amount of feed on a feeding table in the middle of the pond, inducing fishes to concentrate to the middle of the pond, feeding an isolation net suitable for cultivating the fishes at a lower air inlet of the pond, slowly intercepting the fishes to the middle of the pond, forcing all the fishes to be free at the lower air inlet, and finally forming a class enclosure area in the area 1/5-1/3 around the lower air inlet of the cultivating pond, so that cyanobacterial bloom in the class enclosure area is obviously gathered and no fishes swim;
(c) uniformly splashing biological flocculating agents to the enclosure area of the aquaculture pond according to the water usage amount of 30-100 ml/cubic meter;
(d) after 25-60 minutes, a large number of blue algae flocculating constituents floating on the surface of the water body appear, and the blue algae flocculating constituents in the enclosure are completely fished out by using a fishing net of 40-80 meshes;
(e) after removing the isolation net like enclosure, adding a slow-release microcystin degradation catalyst into the culture pond according to the using amount of 1-2 per cubic meter of water; the preparation method of the slow-release microcystin degradation catalyst comprises the following steps: mixing fulvic acid, microalgae powder, feed protease powder and carrageenan according to the mass ratio of 7-8: 1.4-1.6: 0.1-0.2, adding a calcium chloride solution with the mass percentage concentration of 0.5-2% according to the mass ratio of 1: 1.0-1.5, stirring, and then pressing by a tablet press to form a sustained release preparation with the diameter of 2-8 cm;
(f) uniformly sprinkling fish antidotes taking vitamin C as a main component to the aquaculture pond.
In the step (c), the bioflocculant is Bacillus mucilaginosus culture solution.
The invention has the beneficial effects that: the method can overcome the limitations of emergency treatment of the blue algae water in the aquaculture pond by the traditional physical method, chemical method and biological method. When the cyanobacterial bloom in the freshwater fish culture pond is treated in an emergency manner, the method can remarkably reduce the biomass of the cyanobacterial bloom in the culture water body in a very short time, effectively catalyze, degrade and remove microcystin in the culture water body, enhance the immunity of cultured fishes, and further reduce the harm of the cyanobacterial bloom to the cultured fishes fundamentally. The method is efficient and good in ecological safety, can greatly reduce the harm of the pond cyanobacterial bloom to the cultured fishes in a short time, does not cause secondary damage to the cultured fishes, and can be applied to emergency treatment of the cyanobacterial bloom in the freshwater culture pond in a large scale.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments below:
the invention discloses an emergency disposal method for rapidly reducing water bloom hazards of a freshwater fish culture pond, which comprises the following steps:
(a) opening an impeller type aerator in a freshwater fish culture pond which has burst cyanobacterial bloom for 30-60 minutes to force the cyanobacterial bloom to gather at the edge of the culture pond as much as possible, particularly around a lower tuyere of the culture pond;
(b) feeding a small amount of feed on a feeding table in the middle of the pond, inducing fishes to concentrate to the middle of the pond, feeding an isolation net suitable for cultivating the fishes at a lower air inlet of the pond, slowly intercepting the fishes to the middle of the pond, forcing all the fishes to be free at the lower air inlet, and finally forming a class enclosure area in the area 1/5-1/3 around the lower air inlet of the cultivating pond, so that cyanobacterial bloom in the class enclosure area is obviously gathered and no fishes swim;
(c) uniformly splashing biological flocculating agents to the enclosure area of the aquaculture pond according to the water usage amount of 30-100 ml/cubic meter;
(d) after 25-60 minutes, a large number of blue algae flocculating constituents floating on the surface of the water body appear, and the blue algae flocculating constituents in the enclosure are completely fished out by using a fishing net of 40-80 meshes;
(e) after removing the isolation net like enclosure, adding a slow-release microcystin degradation catalyst into the culture pond according to the using amount of 1-2 per cubic meter of water; the preparation method of the slow-release microcystin degradation catalyst comprises the following steps: mixing fulvic acid, microalgae powder, feed protease powder and carrageenan according to the mass ratio of 7-8: 1.4-1.6: 0.1-0.2, adding a calcium chloride solution with the mass percentage concentration of 0.5-2% according to the mass ratio of 1: 1.0-1.5, stirring, and then pressing by a tablet press to form a sustained release preparation with the diameter of 2-8 cm;
(f) uniformly sprinkling fish antidotes taking vitamin C as a main component to the aquaculture pond.
In the step (c), the bioflocculant is Bacillus mucilaginosus (Bacillus mucilaginosus) culture solution, and the preparation method of the culture solution can be seen (Hejia dynasty, et al, environmental engineering journal, 2011,5(7): 1507-: 200 ml of nitrogen-containing culture medium (10 g of sucrose, 0.3 g of yeast extract, CaCO) is filled into a 500 ml triangular flask30.5 g, MgSO47H2O1 g, K2HP041g), inoculating bacillus mucilaginosus after sterilization, placing the bacillus mucilaginosus in a constant temperature shaking table at 30 ℃ and 150 r/min for culturing for 5-6 days, and carrying out amplification culture in a sterile nitrogen-free culture medium according to the inoculum size of 10% (5 g of sucrose, CaCO)30.1 g, MgSO4·7H20.5 g of O, FeCl3·6H2O5 mg, K2HPO42g of glass powder and 1g of glass powder) until the culture solution is a uniform viscous liquid, thus obtaining the biological flocculant;
preferably, the slow-release microcystin degradation catalyst in the step (e) is fulvic acid, microalgae powder, feeding protease powder and carrageenan which are uniformly mixed according to the proportion of 8:1.6:0.2:0.2, calcium chloride solution with the mass percentage concentration of 0.5% is added according to the mass ratio of 1:1.2, the mixture is stirred, then the mixture is pressed into a slow-release preparation with the diameter of 6 cm and the height of 3 cm by a tablet press, the specific preparation method comprises the steps of uniformly mixing fulvic acid, spirulina powder, acidic feeding protease powder (American Jianming) and carrageenan according to the proportion of 8:1.6:0.2:0.2 to obtain a mixture A, then manually uniformly mixing the mixture A and the calcium chloride solution with the mass percentage concentration of 0.5% according to the mass ratio of 1:1.2(W/W) to obtain a mixture B with strong viscosity, then placing the mixture B in a stainless steel mold with the diameter of 6 cm and the height of 4 cm, the mixture B in the die can be pressed into the slow-release microcystin degradation catalyst with the diameter of 6 cm and the height of 3 cm by adopting a hydraulic press with the pressure of 20 tons.
The method comprises the steps of firstly starting an aerator in the freshwater fish culture pond which has developed cyanobacteria bloom to force the cyanobacteria bloom to gather at the edge of the culture pond as much as possible, particularly to gather at the periphery of a downwind port of the culture pond, then forcing all cultured fishes to free at the periphery of the downwind port of the pond (forming a class surrounding area without fish swimming) by adopting a mode of feeding, inducing and putting an isolation net in the middle of the pond to intercept, then flocculating the cyanobacteria bloom in the class surrounding area by adopting a biological flocculant, manually fishing out the flocculated cyanobacteria bloom, and finally removing the isolation net and putting a slow-release microcystin toxin degradation catalyst and a fish antidote into the pond in sequence. The method can remarkably reduce the biomass of the blue algae in the culture water body in a very short time when the blue algae bloom in the freshwater fish culture pond is treated in an emergency, effectively catalyze, degrade and remove the microcystin in the culture water body, enhance the immunity of the cultured fish, and further fundamentally reduce the harm of the blue algae bloom to the cultured fish.
1. Application example of emergency disposal method for quickly and effectively reducing water bloom hazard of freshwater fish culture pond in crucian culture pond
The invention is used for emergently disposing the blue algae bloom in the Zhanren Zhuang crucian carp culture pond (11 mu) in Ninghe area of Tianjin in 2016 (7 months), and the microcystis cell density of the pond reaches (8.46 +/-0.23) × 10 before emergency disposal8The content of cell/l and water-soluble microcystin is 6.43 microgram/l (based on the total amount of MC-L R, MC-RR and MC-YR).
(a) Starting an impeller type aerator for 30-60 minutes to force the cyanobacterial bloom to gather at the edge of the culture pond as much as possible, particularly around a downwind port of the culture pond;
(b) feeding feed on a feeding table in the middle of the pond, attracting crucians to concentrate in the middle of the pond, stopping the crucians after an isolation net is put in a lower air inlet of the pond, forcing all the crucians to be free from the lower air inlet, and finally forming a similar enclosure area around the lower air inlet of the aquaculture pond by about the area 1/4 of the pond;
(c) uniformly splashing a biological flocculant, namely a bacillus mucilaginosus culture solution, to the air inlet below the culture pond according to the water dosage of 80 ml/cubic meter, and obtaining a large number of blue algae flocculants floating on the surface of the water after 50 minutes;
(d) manually fishing out the large sheet-shaped blue algae flocculating constituent in the class enclosure by using an 80-mesh fishing net;
(e) after the separation net outside the enclosure-like barrier is removed, a slow-release microcystin degradation catalyst is put into the culture pond according to the using amount of 2 water bodies per cubic meter;
(f) an antidote, namely vitamin C antidotal warfarin (Nanning Xingxin cattle bioengineering Co., Ltd.) is uniformly sprinkled to the culture pond, the sprinkling dose is 400 g/mu.m, and water is added before sprinkling for complete dissolution.
The implementation of the method of the invention, namely the cell density of the microcystis in the aquaculture water is monitored and found to be (1.39 +/-0.47) × 10 after the antidote is sprinkled for 2 hours8Cell/liter, i.e. the algal cell density decreased by 83.6%; the content of the soluble microcystin in the water is 1.41 micrograms/liter, namely the content of the toxin is reduced by 78.1 percent; meanwhile, the phenomenon of fish death does not occur.
2. Application example of emergency disposal method for rapidly and effectively reducing water bloom hazard of freshwater fish culture pond in carp culture pond
The invention is used for emergency treatment of blue algae bloom in the refined Wuzhen crucian culture pond (about 6 mu) in Xiqing district of Tianjin city in 2016 (8 months), and the cell density of the microcystis algae in the pond reaches (1.26 +/-0.30) × 10 before emergency treatment9The content of cell/l and water-soluble microcystin is 8.25 microgram/l (based on the total amount of MC-L R, MC-RR and MC-YR).
(a) Starting an impeller type aerator for 30-60 minutes to force the cyanobacterial bloom to gather at the edge of the carp culture pond as much as possible when the carp culture pond is exposed to severe cyanobacterial bloom;
(b) feeding feed on a feeding table in the middle of the pond, attracting crucians to concentrate in the middle of the pond, stopping the crucians after an isolation net is put at the edge of the pond, forcing all the crucians to free the edge, and finally forming a similar enclosure area by about the area 1/3 of the pond at the edge of the aquaculture pond;
(c) uniformly splashing a bioflocculant, namely a bacillus mucilaginosus culture solution, to the air inlet below the culture pond according to the water dosage of 100 ml/cubic meter, and after 60 minutes, obtaining a large number of blue algae flocculating constituents floating on the surface of the water;
(d) manually fishing out the large sheet-shaped blue algae flocculating constituents at the edge of the pond by using a 60-mesh fishing net;
(e) after the separation net at the edge of the pond is removed, a slow-release microcystin degradation catalyst is put into the aquaculture pond according to the using amount of 2 water bodies per cubic meter;
(f) an antidote, namely vitamin C antidotal warfarin (Nanning Xingxin cattle bioengineering Co., Ltd.) is uniformly sprinkled to the culture pond, the sprinkling dose is 500 g/mu.m, and water is added before sprinkling for complete dissolution.
The implementation of the method of the invention, namely the cell density of the microcystis in the aquaculture water is monitored and found to be (4.33 +/-0.51) × 10 after the antidote is sprinkled for 2 hours8Cell/liter, i.e. the algal cell density decreased by 65.6%; the content of the soluble microcystin in the water is 0.68 micrograms/liter, namely the content of the toxin is reduced by 92.8 percent; meanwhile, the phenomenon of fish death does not occur.
3. Examples of bioflocculants preparation
(a) The Bacillus mucilaginosus is from algal bacteria storage of marine life institute of China university;
(b) preparing a nitrogen culture solution: 10 g of cane sugar, 0.3 g of yeast extract and CaCO30.5 g, MgSO47H2O1 g, K2HP041g of the bacillus mucilaginosus is dissolved in 200 ml of deionized water, placed in a 500 ml triangular flask, sterilized at high temperature, cooled and inoculated with bacillus mucilaginosus;
(c) culturing the Bacillus mucilaginosus culture solution in a constant temperature shaking table at 30 ℃ and 150 rpm for 6 days;
(d) preparing a nitrogen-free culture solution: sucrose 5 g, CaCO30.1 g, MgSO4·7H20.5 g of O, FeCl3·6H2O5 mg, K2HPO42g of glass powder and 1g of glass powder are dissolved in 2000 ml of deionized water, and the mixture is placed in a 5000 ml triangular flask and sterilized at high temperature;
(e) carrying out expanded culture in a sterile nitrogen-free culture medium according to the inoculation amount of 10% until a culture solution is a uniform viscous liquid, thus obtaining the biological flocculant;
4. preparation example of sustained-release microcystin degradation catalyst
(a) Mixing fulvic acid, Spirulina powder, acidic feed protease powder (American standard) and carrageenan at ratio of 8:1.6:0.2:0.2 to obtain mixture A;
(b) manually stirring and uniformly mixing the mixture A and a calcium chloride solution with the mass percentage concentration of 0.5% according to the mass ratio of 1:1.2(W/W) to obtain a mixture B with strong viscosity;
(c) after the mixture B is placed in a stainless steel die with the diameter of 6 cm and the height of 4 cm, the mixture B in the die can be pressed into the slow-release microcystin degradation catalyst with the diameter of 6 cm and the height of 3 cm by adopting a hydraulic press with the pressure of 20 tons.
The above-mentioned embodiments are only for illustrating the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and to carry out the same, and the present invention shall not be limited to the embodiments, i.e. the equivalent changes or modifications made within the spirit of the present invention shall fall within the scope of the present invention.
Claims (2)
1. An emergency disposal method for rapidly reducing water bloom hazards of a freshwater fish culture pond is characterized by comprising the following steps:
(a) opening an impeller type aerator in a freshwater fish culture pond which has burst cyanobacterial bloom for 30-60 minutes to force the cyanobacterial bloom to gather at the edge of the culture pond as much as possible, particularly around a lower tuyere of the culture pond;
(b) feeding a small amount of feed on a feeding table in the middle of the pond, inducing fishes to concentrate to the middle of the pond, feeding an isolation net suitable for cultivating the fishes at a lower air inlet of the pond, slowly intercepting the fishes to the middle of the pond, forcing all the fishes to be free at the lower air inlet, and finally forming a class enclosure area in the area 1/5-1/3 around the lower air inlet of the cultivating pond, so that cyanobacterial bloom in the class enclosure area is obviously gathered and no fishes swim;
(c) uniformly splashing biological flocculating agents to the enclosure area of the aquaculture pond according to the water usage amount of 30-100 ml/cubic meter;
(d) after 25-60 minutes, a large number of blue algae flocculating constituents floating on the surface of the water body appear, and the blue algae flocculating constituents in the enclosure are completely fished out by using a fishing net of 40-80 meshes;
(e) after removing the isolation net like enclosure, adding a slow-release microcystin degradation catalyst into the culture pond according to the using amount of 1-2 per cubic meter of water; the preparation method of the slow-release microcystin degradation catalyst comprises the following steps: mixing fulvic acid, microalgae powder, feed protease powder and carrageenan according to the mass ratio of 7-8: 1.4-1.6: 0.1-0.2, adding a calcium chloride solution with the mass percentage concentration of 0.5-2% according to the mass ratio of 1: 1.0-1.5, stirring, and then pressing into a sustained-release preparation with the diameter of 2-8 cm by using a tablet press;
(f) uniformly sprinkling fish antidotes taking vitamin C as a main component to the aquaculture pond.
2. The emergency treatment method for rapidly reducing the water bloom hazard in the freshwater fish culture pond according to claim 1, wherein in the step (c), the bioflocculant is a Bacillus mucilaginosus culture solution.
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102879B (en) * | 2018-01-23 | 2019-07-16 | 河海大学 | A kind of separator of Microcystis aeruginosa morphologic species and the separation method of Microcystis aeruginosa morphologic species |
| CN114762492B (en) * | 2022-03-25 | 2024-02-02 | 句容市容河水产养殖有限公司 | Ecological environment regulation and control method for shrimp and crab culture pond |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4894161A (en) * | 1982-07-29 | 1990-01-16 | Solmat Systems, Ltd. | Process for clarifying a liquid using a polymeric substance |
| CN1887742A (en) * | 2005-06-30 | 2007-01-03 | 宝山钢铁股份有限公司 | Controllable fast reservior blue-green alga bloom eliminating method |
| CN102531245A (en) * | 2010-12-22 | 2012-07-04 | 淮海工学院 | Method for treating water bloom blue-green algae harmlessly |
| WO2012102958A2 (en) * | 2011-01-25 | 2012-08-02 | Agrium Advanced Technologies | Controlled release copper sulfate for phytoplankton control |
| CN102774951A (en) * | 2012-08-13 | 2012-11-14 | 江门汇海饲料厂有限公司 | Water body detoxication anti-stress agent for aquaculture and preparation method and application thereof |
| CN102910743A (en) * | 2012-10-25 | 2013-02-06 | 中国水产科学研究院南海水产研究所 | Method for microcystis algae bloom treatment in earth pond cultivation process |
| CN104445446A (en) * | 2013-09-15 | 2015-03-25 | 重庆工商大学 | New technology for river sand modification and algae reduction with combined allelopathic modifier |
| CN106630193A (en) * | 2017-01-05 | 2017-05-10 | 北京清水生态环境工程股份有限公司 | Biological comprehensive prevention and control method for controlling cyanobacterial bloom by microbial preparation |
-
2017
- 2017-05-25 CN CN201710379741.9A patent/CN107176751B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4894161A (en) * | 1982-07-29 | 1990-01-16 | Solmat Systems, Ltd. | Process for clarifying a liquid using a polymeric substance |
| CN1887742A (en) * | 2005-06-30 | 2007-01-03 | 宝山钢铁股份有限公司 | Controllable fast reservior blue-green alga bloom eliminating method |
| CN102531245A (en) * | 2010-12-22 | 2012-07-04 | 淮海工学院 | Method for treating water bloom blue-green algae harmlessly |
| WO2012102958A2 (en) * | 2011-01-25 | 2012-08-02 | Agrium Advanced Technologies | Controlled release copper sulfate for phytoplankton control |
| CN102774951A (en) * | 2012-08-13 | 2012-11-14 | 江门汇海饲料厂有限公司 | Water body detoxication anti-stress agent for aquaculture and preparation method and application thereof |
| CN102910743A (en) * | 2012-10-25 | 2013-02-06 | 中国水产科学研究院南海水产研究所 | Method for microcystis algae bloom treatment in earth pond cultivation process |
| CN104445446A (en) * | 2013-09-15 | 2015-03-25 | 重庆工商大学 | New technology for river sand modification and algae reduction with combined allelopathic modifier |
| CN106630193A (en) * | 2017-01-05 | 2017-05-10 | 北京清水生态环境工程股份有限公司 | Biological comprehensive prevention and control method for controlling cyanobacterial bloom by microbial preparation |
Non-Patent Citations (1)
| Title |
|---|
| 胶质芽孢杆菌、黑曲霉及与沸石联合对模拟废水中Fe3+处理研究;郝建朝等;《环境工程学报》;20110731;第5卷(第7期);全文 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2817333C1 (en) * | 2022-02-15 | 2024-04-15 | федеральное государственное бюджетное образовательное учреждение высшего образования "Пензенский государственный аграрный университет" | Method to prevent water "blooming" with the help of representative of aboriginal fish fauna-young roach-using artificial floating spawning grounds-islands in central russia |
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