CN104445446A - New technology for river sand modification and algae reduction with combined allelopathic modifier - Google Patents
New technology for river sand modification and algae reduction with combined allelopathic modifier Download PDFInfo
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- CN104445446A CN104445446A CN201310418247.0A CN201310418247A CN104445446A CN 104445446 A CN104445446 A CN 104445446A CN 201310418247 A CN201310418247 A CN 201310418247A CN 104445446 A CN104445446 A CN 104445446A
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- Prior art keywords
- algae
- modifier
- sediment
- river sand
- allelopathic
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
Abstract
Belonging to algae removal technologies, the invention relates to a novel algaecide and a new technology for river sand modification and algae reduction with a combined allelopathic modifier. Specifically, natural sediment is adopted as an algae removal carrier, and a natural organic matter (chitosan) is employed as the modifier, after surface coating modification to natural sediment by a combined allelopathic substance (p-hydroxybenzoic acid), the particle surface carrying charge able to neutralize algal cells in water and modify sediment makes algal cells easier to flocculate and gather, and the modifier in the modified sediment can form a network structure to further capture algal cells, and is eventually removed by sedimentation of flocculent bodies or floccules formed by sediment particles. At the same time, as time goes by, allelochemicals released after chitosan hydrolysis can have inhibiting and killing effects on algal growth, thus achieving the purpose of efficient algae removal.
Description
Technical field
This technology is a novel algae removal technology, specifically using natural river sand as removing algae carrier, the object of modification river sand is reached by the coating function of properties-correcting agent (chitosan) to allelochemical (P-hydroxybenzoic acid) and natural river sand, to utilize natural allelochemical to the suppression of frustule regenerative power, kill activity while frustule ability is removed in silt flocculation improve, realize long-term, efficient, harmless except Cutleriales.
Background technology
At present, both at home and abroad except the method for algae, direct method and the large class of indirect method two usually can be divided into.Directly except algae can be divided into again chemical process, physical method and biological method three major types.Indirect method mainly refers to and reaches by the concentration removed or reduce nutritive salt the method controlling algal grown.
(1) direct method:
1, Physical: machinery, clay are except algae, filtration, shading technology, ultrasonic wave, air floatation alga removing etc.;
2, chemical method: chlorine removes algae, ozone removes algae, potassium permanganate removes algae, dioxide peroxide removes algae, conventional flocculation agent has Tai-Ace S 150, polyacrylamide, ferric sulfate, iron trichloride etc.;
3, biological process: with algae algae, microbial flocculant except algae, biocontrol agent, higher plant restraint algae, fish control algae etc.
In addition, foreign literature reports the algae method using fine sand to come in filtered water as filtration medium, also has slow huskyly to filter for removing the algae in the sewage of pond.
(2) indirect method
While basin countermeasure advances, introduce (comprising in reservoir, river) countermeasure in lake.This countermeasure comprises two aspects, one is desilting, the organic substance be deposited in for a long time in Ha Noi or lake is removed, remove internal contamination source, be implemented into a certain degree in basin countermeasure, this measure is practical, but too early implements, inherently bring and again deposit, efficiency will reduce.Second is exactly the nutritive salt consumed by all means in water, the nutrient concentration in lake water is reduced in a large number, to control the breeding of algae.The engineering measures such as this method comprises balancing reservoir operation scheme, diversion dilution, and aquatic plant purification, optimum algae reproduction etc.On purpose allow a certain optimum algae or aquatic organism amount reproduction in a certain specific region, then the means being equipped with other eliminate the content of nutritive salt in water system by the collection from the algae in river, lake or waterplant.
This research is removing on the basis of algae theory, propose using natural silt as except algae carrier, the coating function of properties-correcting agent (chitosan) to allelochemical (P-hydroxybenzoic acid) and natural river sand reaches the object of modification river sand.This research is through sci-tech novelty-search, and domestic and international pertinent literature according to the retrieval shows, does not also find to remove algae with the silt of surface modification as except algae carrier.
Summary of the invention
At present, conventional algicide mostly is flocculation agent.These materials dosage in the process removing algae is comparatively large, and produces certain negative impact to environment.Therefore, a kind of efficient environmentally friendly algicide of exploitation is significant.
For achieving the above object, the innovation invent the technical problem that will solve mainly comprise microcystic aeruginosa cultivation, the test of river sand grating, organic modifiers choice experiment, allelochemical research experiment, utilize organic modifiers and allelochemical to carry out surface modification experiment and laboratory and outdoor pilot scale enclosure to river sand to fall the research of algae experiment effect.
The technical scheme that its technical problem of the innovation invention solution adopts mainly comprises following steps:
First pre-treatment is carried out to river sand, then find out the correlative factor affecting modification river sand, experimental design is carried out to it, chitosan and the P-hydroxybenzoic acid of determining to fall algae best results carry out modification to river sand, finally laboratory and outdoor pilot scale enclosure are carried out to modification river sand and fall the research of algae experiment effect, according to test result, constantly improve preparation parameter and condition, make it fall algae effect and reach best.
The innovation beneficial effect of the invention is, by harmless, that flocculation ability is extremely strong chitosan and Secondary Metabolite Production in Plants (allelochemical) and to algal grown have suppression and kill activity P-hydroxybenzoic acid combination after coating modification is carried out to sand surface, river, efficiently fall in the process of algae can not accumulate in the environment in realization, new environmental pollutants can not be introduced, can not impact water body environment, more environmental protection.
Embodiment:
1st step: the sample pretreatment before first being tested by acquired natural silt, object removes the organic matter in silt.Strong oxidizer hydrogen peroxide and deionized water is adopted repeatedly to clean sample.The H of 30% is constantly added in silt sample
2o
2the stirred in water bath of 50 DEG C, question response fully no longer include bubble emerge and heat release time, stop add H
2o
2, continuation heating in water bath cleaned repeatedly with deionized water after half an hour, outwelled supernatant liquid and filtered, and in 60 DEG C of oven for drying, grinding, crossed 200 orders (74um) sample sieve and sieved rear stand-by.
2nd step: adopt BG-11 substratum, be divided in by 1L substratum in the Erlenmeyer flask of 3 1L, uses sodium hydroxide solution or hydrochloric acid soln to regulate and is adjusted to the pH of substratum in each Erlenmeyer flask to about 7.1.To configure the Erlenmeyer flask tampon beyond the Great Wall of substratum subsequently, moist heat sterilization 30min under the pressure of 1.05kg/cm2, naturally cools to room temperature by the substratum after sterilizing, for subsequent use.Well-grown algae liquid is inoculated in and is cooled in the substratum of room temperature by the inoculum size by 10%, is placed in biochemical cultivation case and cultivates.The operating parameter of biochemical cultivation case is: culture temperature is 25 ± 1 DEG C, intensity of illumination 2000lx, and light dark period is 14h:10h.In the process of cultivating, to Erlenmeyer flask be shaken every day and convert the putting position of Erlenmeyer flask, making the algae in bottle can all receive uniform light.
3rd step: the colorimetric cylinder of 6 100ml is cleaned, dries, be placed on Colorimetric cylinder holder for subsequent use.Be respectively charged into the algae liquid that 100ml is in increased logarithmic phase often propping up in colorimetric cylinder.Get a colorimetric cylinder as blank, in all the other 5 colorimetric cylinders, add 0.05g P-hydroxybenzoic acid, 0.05g linolic acid, 0.05g anthraquinone, 0.05g pyrocatechol and 0.05g gallic acid respectively, shake up rear leaving standstill.Subsequently above-mentioned 6 colorimetric cylinders are put into constant incubator to cultivate 24 hours (culture temperature is (25 ± 1) DEG C, and intensity of illumination is 2000lx, and Light To Dark Ratio is 14h:10h), measure algae density after taking-up under the microscope, calculate alga-killing rate.Often organize experiment and do three Duplicate Samples.
4th step: get a clean 100ml beaker, use distilled water rinse.Respectively to the hydrochloric acid soln, the 0.1g chitosan that add 10ml1% in beaker, stir with glass stick, chitosan is all dissolved in dilute hydrochloric acid.Add 0.05g P-hydroxybenzoic acid respectively again, 1g river sand, stirs, for subsequent use.Get 2 clean 100ml colorimetric cylinders, add the algae liquid that 100ml is in logarithmic phase respectively.A colorimetric cylinder, as blank sample, adds the above-mentioned river sand suspension liquid of 1ml in another colorimetric cylinder, leave standstill.(culture temperature is (25 ± 1) DEG C 2 colorimetric cylinders to be placed on cultivation in constant incubator, intensity of illumination is 2000lx, Light To Dark Ratio is 14h:10h), after 24 hours, 48 hours, 72 hours, measure the algae density of algae liquid in each colorimetric cylinder respectively, calculate alga-killing rate.Often organize experiment and do three Duplicate Samples.
Accompanying drawing illustrates:
Fig. 1: the river sand sample of collection
Fig. 2: the microcystic aeruginosa reaching logarithmic phase
Fig. 3: to the growth effect of algae during P-hydroxybenzoic acid independent role
Fig. 4: the growth effect to algae after chitosan combination allelopathic substance modification river sand
Fig. 5: electron micrograph when river sand is unmodified
Fig. 6: the electron micrograph that river sand is modified
Fig. 7: outdoor drum lab scale testing ground algae pollution situation
Fig. 8: the design sketch after algae falls in outdoor drum lab scale test
Fig. 9: algae effect contrast figure falls in outdoor pilot scale Enclosure experiments
Figure 10: water transparency design sketch after algae falls in outdoor pilot scale Enclosure experiments.
Claims (1)
1. one kind is combined the new technology that algae falls in allelopathic modifier modification river sand, it is characterized in that, with natural silt for removing algae carrier, take chitosan as properties-correcting agent, combination allelochemical (P-hydroxybenzoic acid) carries out surface coating modification to natural silt, changes the algicide of silt surface properties.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310418247.0A CN104445446A (en) | 2013-09-15 | 2013-09-15 | New technology for river sand modification and algae reduction with combined allelopathic modifier |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310418247.0A CN104445446A (en) | 2013-09-15 | 2013-09-15 | New technology for river sand modification and algae reduction with combined allelopathic modifier |
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| CN104445446A true CN104445446A (en) | 2015-03-25 |
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| CN201310418247.0A Pending CN104445446A (en) | 2013-09-15 | 2013-09-15 | New technology for river sand modification and algae reduction with combined allelopathic modifier |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107176751A (en) * | 2017-05-25 | 2017-09-19 | 天津农学院 | A kind of emergence treating method of quick reduction Fish-water Fish Farming pond wawter bloom harm |
| CN107892367A (en) * | 2017-11-16 | 2018-04-10 | 安徽师范大学 | Magnetic efficient algae removal agent and its preparation method and application |
| CN108558026A (en) * | 2018-04-20 | 2018-09-21 | 裴泽民 | A kind of preparation method of algicide |
Citations (4)
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|---|---|---|---|---|
| CN102139942A (en) * | 2010-02-02 | 2011-08-03 | 中国科学院生态环境研究中心 | Method for high-efficiency flocculation of algal blooms by modified clay, soil or sand |
| CN102334487A (en) * | 2010-07-23 | 2012-02-01 | 北京林业大学 | Vegetable tannin algae inhibitor and method for inhibiting overgrowth of algae in eutrophicated fresh water area |
| CN102963940A (en) * | 2012-11-23 | 2013-03-13 | 中国科学院水生生物研究所 | Addition method for enhancing algae removal effect of allelochemicals |
| CN203095654U (en) * | 2013-03-19 | 2013-07-31 | 重庆大学 | Multifunctional mobile algae inhibition and removal platform suitable for river channel type water bodies |
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2013
- 2013-09-15 CN CN201310418247.0A patent/CN104445446A/en active Pending
Patent Citations (4)
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| CN102139942A (en) * | 2010-02-02 | 2011-08-03 | 中国科学院生态环境研究中心 | Method for high-efficiency flocculation of algal blooms by modified clay, soil or sand |
| CN102334487A (en) * | 2010-07-23 | 2012-02-01 | 北京林业大学 | Vegetable tannin algae inhibitor and method for inhibiting overgrowth of algae in eutrophicated fresh water area |
| CN102963940A (en) * | 2012-11-23 | 2013-03-13 | 中国科学院水生生物研究所 | Addition method for enhancing algae removal effect of allelochemicals |
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Non-Patent Citations (1)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107176751A (en) * | 2017-05-25 | 2017-09-19 | 天津农学院 | A kind of emergence treating method of quick reduction Fish-water Fish Farming pond wawter bloom harm |
| CN107176751B (en) * | 2017-05-25 | 2020-07-24 | 天津农学院 | Emergency disposal method for rapidly reducing water bloom hazard of freshwater fish culture pond |
| CN107892367A (en) * | 2017-11-16 | 2018-04-10 | 安徽师范大学 | Magnetic efficient algae removal agent and its preparation method and application |
| CN108558026A (en) * | 2018-04-20 | 2018-09-21 | 裴泽民 | A kind of preparation method of algicide |
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Application publication date: 20150325 |