CN105861395B - It is a kind of it is effective inhibit wawter bloom microcystic aeruginosa composite bacteria agent DH-1 and its application - Google Patents
It is a kind of it is effective inhibit wawter bloom microcystic aeruginosa composite bacteria agent DH-1 and its application Download PDFInfo
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- CN105861395B CN105861395B CN201610421245.0A CN201610421245A CN105861395B CN 105861395 B CN105861395 B CN 105861395B CN 201610421245 A CN201610421245 A CN 201610421245A CN 105861395 B CN105861395 B CN 105861395B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
Abstract
The invention discloses a kind of composite bacteria agent DH-1 for effectively inhibiting wawter bloom microcystic aeruginosa and its applications.The bacterium and the atypical bacterium for wishing Salmonella (Alishewanella) category that goes out that bacterium, the De Wosi Salmonella (Devosia) that its bacterium for containing pseudomonad (Pseudomona) category, the bacterium of protozoa (Shinella) category, hot monad (Thermomonas) belong to belong to.The present invention is by putting into specific functional microorganism-composite bacteria agent DH-1 into water body, play the effect of microorganism aquatic Tiny ecosystem adjusting and " phycomycete interaction ", to reach the microorganism of inhibition (removal) microcystic aeruginosa except algae method, with at low cost, highly-safe and do not destroy the characteristic of water ecological setting, have important practical significance to microcystic aeruginosa wawter bloom water body is administered.
Description
Technical field
The invention belongs to microbe application fields, and in particular to a kind of composite bacteria agent for effectively inhibiting wawter bloom microcystic aeruginosa
DH-1 and its application.
Background technique:
With the development of urbanization and industrial and agricultural production, largely rich in the industrial wastewater of nutriments such as nitrogen (N), phosphorus (P),
Sanitary sewage flows into the slow flow water bodies such as lake, river, is enriched with nutriment in water rapidly, and algae fulminant increases in water, blue
Algae wawter bloom frequently occurs, and causes water body serious eutrophication, brings not to Water quality, water safety and landscape environment etc.
Benefit influences.Microcystic aeruginosa (Microcystis aeruginosa) is most common algae and China cyanobacteria for causing wawter bloom
The main algae of wawter bloom, special physiological ecological feature make it easily become advantage algae in eutrophication water and cause
Cyanobacterial bloom is broken out.Microcystic aeruginosa can release hepatotoxin in cell growth and death process, which is a kind of strong
Therefore carcinogenic promoting agent, the health of the indirect hazard mankind very harmful to aquatile are administered cyanobacterial bloom pollution and are had become urgently
It solves the problems, such as.
The administering method of cyanobacterial bloom mainly has physical method and chemical method at present, these methods have the defects that it is certain, such as
Physical method is at high cost, needs a large amount of manpower and material resources and processing capacity limited.Chemical method be easy to cause secondary pollution, selects toxicity poor
Or small area waters can only be adapted to.Therefore, actively finding novel, efficient, environmentally friendly cyanobacterial bloom control method has very
Important meaning.
Summary of the invention:
The object of the present invention is to provide a kind of composite bacteria agent DH-1 that can effectively inhibit wawter bloom microcystic aeruginosa and its applications.
The composite bacteria agent DH-1 that can effectively inhibit wawter bloom microcystic aeruginosa of the invention, which is characterized in that contain false unit cell
The bacterium of Pseudomonas (Pseudomona sp.), the bacterium of protozoa category (Shinella sp.), hot zygosaccharomyces
The bacterium of (Thermomonas sp.), the bacterium of De Wosi Bordetella (Devosia sp.) and atypical uncommon Bordetella of going out
The bacterium of (Alishewanella sp.).
It is preferred that described can effectively inhibit in the composite bacteria agent DH-1 of wawter bloom microcystic aeruginosa, the bacterium of pseudomonas,
The bacterial population of bacterium, the bacterium of hot zygosaccharomyces, the bacterium of De Wosi Bordetella and atypical uncommon Bordetella of going out that protozoa belongs to
Amount is than being 160:27:8:3:1.
It is preferred that the bacterium of the pseudomonas is preferably pseudomonas mendocina (Pseudomonas
Mendocina) ACCC01078 or pseudomonad (Pseudomonas sp.) FIM-20151013001；The protozoa
The bacterium of category is protozoa (Shinella sp.) ACCC19821 or protozoa (Shinella sp.) FIM-
20151013002；The bacterium of the hot zygosaccharomyces is hot monad (Thermomonas sp.) FIM-20151013003；
The bacterium of the De Wosi Bordetella is De Wosi Salmonella (Devosia honganense) ACCC19737 or De Wosi Salmonella
(Devosia sp.) FIM-20151013004, the bacterium of the uncommon Bordetella of going out of the abnormal type are preferably atypical uncommon Salmonella of going out
Uncommon Salmonella (Alishewanella sp.) FIM- that goes out of (Alishewanella fetalis) ACCC10045 or atypical
20151013005。
A second object of the present invention is to provide the preparations for the composite bacteria agent DH-1 that can effectively inhibit wawter bloom microcystic aeruginosa
Method, which is characterized in that bacterium, the bacterium of hot zygosaccharomyces, De Wosishi for belonging to the bacterium of pseudomonas, protozoa
After the bacterium solution mixing of the bacterium of the bacterium of Pseudomonas and atypical uncommon Bordetella of going out, bacteria-removing liquid is gone to leave thallus, thallus is compound bacteria
Agent DH-1.
It is preferred that being bacterium, the bacterium of hot zygosaccharomyces, De Wo for respectively belonging to the bacterium of pseudomonas, protozoa
The bacterium of the bacterium of this Bordetella and atypical uncommon Bordetella of going out is fermented respectively, and being then diluted to cell concentration is 1 × 108
A/ml, then by 1 × 108Bacterium, the bacterium of hot zygosaccharomyces, moral that the bacterium of the pseudomonas of a/ml, protozoa belong to
The bacterium of the bacterium of Butterworth Bordetella and atypical uncommon Bordetella of going out is uniformly mixed according to volume ratio 160:27:8:3:1, is then centrifuged for
Supernatant is removed, leaves thallus, thallus is composite bacteria agent DH-1.
Third object of the present invention is to provide can effectively inhibit the composite bacteria agent DH-1 of wawter bloom microcystic aeruginosa inhibiting
Application in wawter bloom microcystic aeruginosa.
The present invention rationally compounds wawter bloom microcystic aeruginosa function bacterium according to microbial ecology principle and method,
Obtaining has the good composite bacteria agent DH-1 for inhibiting (removal) effect to microcystic aeruginosa, has the value of popularization and application.
It is aquatic micro- to play microorganism by putting into specific functional microorganism-composite bacteria agent DH-1 into water body by the present invention
The effect of Ecological regulation and " phycomycete interaction ", to reach the microorganism of inhibition (removal) microcystic aeruginosa except algae method, have at
This is low, highly-safe and does not destroy the characteristic of water ecological setting, has important show to microcystic aeruginosa wawter bloom water body is administered
Sincere justice.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
Pseudomonad (Pseudomonas sp.) FIM-20151013001, protozoa used in the following embodiment
(Shinella sp.) FIM-20151013002, hot monad (Thermomonas sp.) FIM-20151013003, De Wosi
Salmonella (Devosia sp.) FIM-20151013004 and atypical uncommon Salmonella (Alishewanella sp.) FIM- that goes out
20151013005, both from Fujian Microorganism Inst., are preserved in Fujian Microorganism Inst., are preserved in Fujian Province
The bacterial strain of institute of microbiology be it is for sale, these bacterial strains are also for sale, therefore before the applying date, existing skill
Anyone in art can buy these bacterial strains.And above-mentioned bacterial strains had been proposed on webpage before the applying date
(http://www.fjim.org.cn/kytd/ktz/getNews.jsp? newsid=220), bacterial strain the applicant also holds
Have, and guarantees to provide in 20 years to the public from the applying date.
Embodiment 1:
1, inclined-plane culture
By pseudomonad (Pseudomonas sp.) FIM-20151013001, protozoa (Shinella sp.) FIM-
20151013002, hot monad (Thermomonas sp.) FIM-20151013003, De Wosi Salmonella (Devosia sp.)
FIM-20151013004 and atypical uncommon go out Salmonella (Alishewanella sp.) FIM-20151013005 and P. aeruginosa
Bacterium (Pseudomonas aeruginosa), pseudomonas putida (Pseudomonas putida) and pseudomonas mendocina
(Pseudomonas mendocina) is seeded on nutrient agar, in 28 DEG C of 2~3d of incubator culture.
Nutrient agar formula: peptone 10g, beef extract 3g, sodium chloride 5g, 15~20g of agar, distilled water
1000mL, after mixing, 121 DEG C of high pressure sterilization 15min.
2, fermented and cultured
The thallus for provoking the above-mentioned bacterial strains on a small amount of slant medium respectively with oese accesses in fermentation medium, in 28
DEG C, 2~3d of shaken cultivation on the shaking table of 150r/min.Bacterium solution is diluted with fermentation medium, makes its concentration 1 × 108
~109A/ml.Fermentative medium formula (g/L): tryptone 10.0, yeast powder 5.0, sodium chloride 10.0, surplus are water, pH
Naturally, after mixing by above-mentioned composition, sterilizing spare.
3, the preparation of composite bacteria agent
By 1 × 108The pseudomonad bacterium solution of a/ml, 1 × 108The protozoa bacterium solution of a/ml, 1 × 108The heat of a/ml
Monad bacterium solution, 1 × 108The De Wosi Salmonella bacterium solution of a/ml and 1 × 108The uncommon Salmonella bacterium solution of going out of the abnormal type of a/ml, according to volume
Volume ratio than 160:27:8:3:1 is uniformly mixed, and 8000r/min is centrifuged 5min, is abandoned supernatant and is obtained thallus, as compound bacteria
This composite bacteria agent is named as composite bacteria agent DH-1 by agent.
2 composite bacteria agent DH-1 of embodiment and pseudomonas strain test the inhibiting effect of microcystic aeruginosa
Certain permanently happy cyanobacterial bloom river water of Foochow is acquired, which is verdigris color Microcystis aeruginosa.River water is in advance through 8
Layer filtered through gauze removes large particulate matter.2L river water is measured, is added in 5L beaker, while 1L BG-11 culture medium is added, stirs
It is even to be mixed.Not add the group of bacterium as blank control group (CK), to add composite bacteria agent DH-1, the verdigris of embodiment 1 respectively
Pseudomonad, pseudomonas putida, pseudomonas mendocina, protozoa (Shinella sp.) FIM-20151013002, heat
Monad (Thermomonas sp.) FIM-20151013003, De Wosi Salmonella (Devosia sp.) FIM-20151013004
Group with atypical uncommon Salmonella (Alishewanella sp.) FIM-20151013005 that goes out is experimental group, is all provided with three Duplicate Samples,
Pseudomonas aeruginosa, pseudomonas putida, pseudomonas mendocina, protozoa (Shinella sp.) FIM-
20151013002, hot monad (Thermomonas sp.) FIM-20151013003, De Wosi Salmonella (Devosia sp.)
The thallus additive amount of FIM-20151013004 and atypical uncommon Salmonella (Alishewanella sp.) FIM-20151013005 that goes out with
The additive amount of composite bacteria agent DH-1 is identical.Important suction due to chlorophyll a (chlorophyll-a, chl-a) as alga cells
Photopigment plays absorption in photosynthesis and transmits the key effect of luminous energy, plays great influence to the growth of algae, is plant
One of important indicator of upgrowth situation, so using the content of chlorophyll a as the evaluation index of algae content.Using acetone extraction
Spectrophotometry (People's Republic of China's water conservancy industry standard (SL 88-2012)) measures Chlorophyll-a Content.
Chlorophyll-a Content in the initial water body of measured in advance.Experimental group adds compound bacteria by 1 ‰ percent by volume respectively
Agent DH-1, pseudomonas aeruginosa, pseudomonas putida, pseudomonas mendocina, protozoa (Shinella sp.) FIM-
20151013002, hot monad (Thermomonas sp.) FIM-20151013003, De Wosi Salmonella (Devosia sp.)
FIM-20151013004 and atypical uncommon Salmonella (Alishewanella sp.) FIM-20151013005 that goes out, bottom is equipped with aeration
Head stops 2h using intermittent aerating, i.e. exposure 2h, and experiment carries out in illumination box, and condition of culture is (25 ± 1) DEG C, Light To Dark Ratio
(the ratio between light application time and no light time) is 12:12, intensity of illumination 2000lux.Timing is observed.Water body is measured after culture 8d
In Chlorophyll-a Content.
The experimental results showed that (table 1), after cultivating 8d, blank control group (CK) and addition composite bacteria agent DH-1, verdigris are false
Monad, pseudomonas putida, pseudomonas mendocina, protozoa (Shinella sp.) FIM-20151013002, heat are single
Born of the same parents bacterium (Thermomonas sp.) FIM-20151013003, De Wosi Salmonella (Devosia sp.) FIM-20151013004 and
Chlorophyll a average content in the processing group water body of uncommon Salmonella (Alishewanella sp.) FIM-20151013005 that goes out of abnormal type
Respectively 1.349 μ g/L, 0.21 μ g/L, 0.60 μ g/L, 0.69 μ g/L, 0.63 μ g/L, 1.01 μ g/L, 0.98 μ g/L, 1.07 μ g/
L and 0.95 μ g/L.Compared with the 0.29 μ g/L of Chlorophyll-a Content in initial water body, the leaf in blank control group (CK) water body is green
Plain a content has significant rising, illustrates that the microcystic aeruginosa after 8d is cultivated in blank control group mushrooms out breeding, content
It significantly improves.Compared with blank control group (CK), it is added to pseudomonas pseudomonas aeruginosa, pseudomonas putida, Men Duo
Chlorophyll-a Content in the processing group water body of Sa pseudomonad has rising by a small margin, illustrates pseudomonas P. aeruginosa
Bacterium, pseudomonas putida and pseudomonas mendocina have certain inhibitory effect to microcystic aeruginosa, and add protozoa
(Shinella sp.) FIM-20151013002, hot monad (Thermomonas sp.) FIM-20151013003, De Wosi
Salmonella (Devosia sp.) FIM-20151013004 and atypical uncommon Salmonella (Alishewanella sp.) FIM- that goes out
Chlorophyll-a Content in 20151013005 processing group water body has certain rising, illustrates protozoa (Shinella sp.)
FIM-20151013002, hot monad (Thermomonas sp.) FIM-20151013003, De Wosi Salmonella (Devosia
Sp.) FIM-20151013004 and atypical wish go out Salmonella (Alishewanella sp.) FIM-20151013005 to verdigris micro-capsule
Algae does not have significant inhibitory effect, and adds the processing group and blank control group (CK) and addition verdigris vacation of composite bacteria agent DH-1
Monad, pseudomonas putida are compared with the processing group of pseudomonas mendocina, and the Chlorophyll-a Content variation in water body is unknown
It is aobvious, illustrate that composite bacteria agent DH-1 has strong inhibiting effect to wawter bloom microcystic aeruginosa, composite bacteria agent DH-1 is than single vacation
Pseudomonas species strain has the effect of more preferably inhibiting wawter bloom microcystic aeruginosa.
Suppression result of 1 experimental bacteria of table to microcystic aeruginosa
BG11 nutrient media components: NaNO31.5g, K2HPO40.04g, MgSO4·7H2O 0.075g, CaCl2·7H2O
0.036g, Na2CO30.02g, citric acid 0.006g, ironic citrate 0.006g, trace element solution A5 1ml, ampicillin
(final concentration) 50 μ g/mL, water are settled to 1000mL.Trace element solution A5 component: H3BO42.86g MnCl2·
4H2O1.81g, ZnSO40.222g, Na2MoO40.39g, CuSO4·5H2O 0.079g, Co (NO3)2·6H2O 49.4g, water
It is settled to 1000ml.
The present invention can also be belonged to other bacteriums of pseudomonad (Pseudomona) category, protozoa (Shinella)
Other bacteriums, hot monad (Thermomonas) belong to other bacteriums, De Wosi Salmonella (Devosia) belong to other bacteriums and
Other bacteriums of uncommon Salmonella (Alishewanella) category that goes out of abnormal type, such as by the pseudomonad (Pseudomonas in embodiment 1
Sp.) FIM-20151013001 replaces with pseudomonas mendocina (Pseudomonas mendocina) ACCC01078, Shen Shi
Bacillus (Shinella sp.) FIM-20151013002 replaces with protozoa (Shinella sp.) ACCC19821, De Wosi
Salmonella (Devosia sp.) FIM-20151013004 replaces with De Wosi Salmonella (Devosia honganense)
ACCC19737, uncommon Salmonella (Alishewanella sp.) FIM-20151013005 that goes out of abnormal type replace with atypical uncommon Salmonella of going out
(Alishewanella fetalis) ACCC10045, is then prepared composite bacteria agent DH-1 according to the method for embodiment 1
(2), then carry out inhibiting the experiment of wawter bloom microcystic aeruginosa according to the method described above, experimental result shows composite bacteria agent DH-1 (2)
There is strong inhibiting effect to wawter bloom microcystic aeruginosa, composite bacteria agent DH-1 (2) has than single pseudomonas strain
More preferably inhibit the effect of wawter bloom microcystic aeruginosa.The pseudomonas mendocina (Pseudomonas mendocina)
ACCC01078, protozoa (Shinella sp.) ACCC19821, De Wosi Salmonella (Devosia honganense)
ACCC19737, atypical uncommon Salmonella (Alishewanella fetalis) ACCC10045 that goes out are preserved in Chinese agriculture microorganism
Culture presevation administrative center can buy therefrom.
Claims (3)
1. a kind of composite bacteria agent DH-1 that can effectively inhibit wawter bloom microcystic aeruginosa, which is characterized in that contain pseudomonas
The bacterium of (Pseudomona sp.), the bacterium of protozoa category (Shinella sp.), hot zygosaccharomyces (Thermomonas
Sp. the bacterium of bacterium, De Wosi Bordetella (Devosia sp.)) and atypical wish go out Bordetella (Alishewanella sp.)
Bacterium；The bacterium of the pseudomonas is pseudomonad (Pseudomonas sp.) FIM-20151013001；It is described
Protozoa belong to bacterium be protozoa (Shinella sp.) FIM-20151013002；The hot zygosaccharomyces
Bacterium is hot monad (Thermomonas sp.) FIM-20151013003；The bacterium of the De Wosi Bordetella is fertile for moral
This Salmonella (Devosia sp.) FIM-20151013004, the bacterium of the uncommon Bordetella of going out of the abnormal type are atypical uncommon Salmonella of going out
(Alishewanella sp.)FIM-20151013005；The composite bacteria agent that wawter bloom microcystic aeruginosa can effectively be inhibited
In DH-1, the bacterium of pseudomonas, the bacterium that protozoa belongs to, the bacterium of hot zygosaccharomyces, De Wosi Bordetella bacterium
Bacterial number ratio with atypical uncommon Bordetella of going out is 160:27:8:3:1.
2. a kind of preparation method of the composite bacteria agent DH-1 described in claim 1 that can effectively inhibit wawter bloom microcystic aeruginosa,
Be characterized in that, respectively by the bacterium of pseudomonas, protozoa belong to bacterium, hot zygosaccharomyces bacterium, De Wosi Salmonella
The bacterium of the bacterium of category and atypical uncommon Bordetella of going out is fermented respectively, and being then diluted to cell concentration is 1 × 108A/ml, then
By 1 × 108The bacterium of the pseudomonas of a/ml, the bacterium that protozoa belongs to, hot zygosaccharomyces bacterium, De Wosi Salmonella
The bacterium of the bacterium of category and atypical uncommon Bordetella of going out is uniformly mixed according to volume ratio 160:27:8:3:1, is then centrifuged in removal
Clearly, thallus is left, thallus is composite bacteria agent DH-1.
3. the composite bacteria agent DH-1 described in claim 1 that can effectively inhibit wawter bloom microcystic aeruginosa is inhibiting wawter bloom verdigris micro-capsule
Application in algae.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102719383A (en) * | 2012-06-29 | 2012-10-10 | 武汉工业学院 | Bacillus amyloliquefaciens, inoculant and application thereof |
| CN103131637A (en) * | 2013-02-25 | 2013-06-05 | 黑龙江八一农垦大学 | Bacteria compsite inoculum used in lignin decomposition |
| CN105176868A (en) * | 2015-09-08 | 2015-12-23 | 福建省微生物研究所 | Complex bactericide for efficiently purifying sewage anaerobic outlet water of pig farm and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102719383A (en) * | 2012-06-29 | 2012-10-10 | 武汉工业学院 | Bacillus amyloliquefaciens, inoculant and application thereof |
| CN103131637A (en) * | 2013-02-25 | 2013-06-05 | 黑龙江八一农垦大学 | Bacteria compsite inoculum used in lignin decomposition |
| CN105176868A (en) * | 2015-09-08 | 2015-12-23 | 福建省微生物研究所 | Complex bactericide for efficiently purifying sewage anaerobic outlet water of pig farm and preparation method and application thereof |
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